AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0592150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592150 MtrunA17_Chr4g0010271 86.000 150 0 1 1 129 70 219 3.42e-88 255
Msa0592150 MtrunA17_Chr1g0169861 64.000 150 33 1 1 129 120 269 1.55e-67 204
Msa0592150 MtrunA17_Chr2g0296021 61.745 149 36 1 2 129 71 219 1.79e-62 190
Msa0592150 MtrunA17_Chr7g0237561 72.000 150 21 1 1 129 70 219 6.23e-60 184
Msa0592150 MtrunA17_Chr4g0043521 67.200 125 20 1 2 105 73 197 8.96e-56 173
Msa0592150 MtrunA17_Chr4g0010211 94.118 85 5 0 1 85 9 93 2.13e-54 166
Msa0592150 MtrunA17_Chr7g0237521 65.873 126 22 1 1 105 70 195 2.08e-53 167
Msa0592150 MtrunA17_Chr2g0291651 63.200 125 25 1 2 105 75 199 8.16e-53 166
Msa0592150 MtrunA17_Chr1g0169851 56.716 134 22 1 1 98 21 154 2.34e-48 152
Msa0592150 MtrunA17_Chr1g0210521 42.857 168 56 2 2 129 71 238 4.72e-44 144
Msa0592150 MtrunA17_Chr5g0441741 33.562 146 74 3 2 126 69 212 2.06e-21 85.1
Msa0592150 MtrunA17_Chr4g0026121 29.730 148 81 4 2 128 69 214 4.94e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592150 AT1G04760.2 66.667 150 29 1 1 129 70 219 1.38e-63 193
Msa0592150 AT1G04760.1 66.667 150 29 1 1 129 70 219 1.38e-63 193
Msa0592150 AT1G04750.1 71.333 150 22 1 1 129 70 219 6.94e-61 186
Msa0592150 AT2G32670.1 70.000 150 24 1 1 129 135 284 1.86e-60 187
Msa0592150 AT2G33120.1 70.667 150 23 1 1 129 70 219 5.56e-60 184
Msa0592150 AT2G33120.2 70.667 150 23 1 1 129 78 227 6.87e-60 184
Msa0592150 AT1G04750.2 70.922 141 20 1 10 129 1 141 8.03e-56 171
Msa0592150 AT1G04750.4 70.922 141 20 1 10 129 1 141 8.03e-56 171
Msa0592150 AT1G04750.3 70.922 141 20 1 10 129 1 141 8.03e-56 171
Msa0592150 AT2G33110.1 62.329 146 38 1 1 129 70 215 1.51e-47 152
Msa0592150 AT3G54300.3 41.667 168 58 2 2 129 71 238 6.54e-42 139
Msa0592150 AT3G54300.2 41.667 168 58 2 2 129 71 238 6.54e-42 139
Msa0592150 AT3G54300.1 41.667 168 58 2 2 129 71 238 6.54e-42 139
Msa0592150 AT2G33110.2 69.512 82 25 0 1 82 70 151 2.26e-38 128
Msa0592150 AT4G15780.1 65.263 95 33 0 2 96 72 166 1.34e-37 126
Msa0592150 AT5G11150.1 36.000 150 69 6 2 128 70 215 6.70e-23 89.7
Msa0592150 AT5G11150.2 35.811 148 68 6 2 126 70 213 7.81e-23 89.4
Msa0592150 AT5G22360.1 33.784 148 75 4 2 128 70 215 2.94e-22 87.8
Msa0592150 AT4G32150.1 32.192 146 76 3 2 126 69 212 3.00e-19 80.1
Msa0592150 AT2G33120.3 63.043 92 13 1 59 129 46 137 9.57e-18 74.3
Msa0592150 AT3G24890.2 42.553 94 27 1 44 110 14 107 5.45e-17 72.0
Msa0592150 AT2G25340.1 30.612 147 77 4 2 126 69 212 1.09e-14 67.8

Find 21 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTCTTAACAAAGAATT+TGG 0.110351 4_2:-17287582 Msa0592150:CDS
GCTCAAGTTTCTGAAGTTAA+AGG 0.200850 4_2:-17287401 Msa0592150:CDS
TGCTGCAGGCACAAGATTTC+AGG 0.224795 4_2:-17286631 Msa0592150:intron
ACCACATCTTTCTCCTCAAT+TGG 0.292197 4_2:+17286597 None:intergenic
TGCATTTGCTCCTTCAACTT+TGG 0.326828 4_2:+17287473 None:intergenic
ATACGTTCAAGAAAAGCTAT+TGG 0.353729 4_2:+17287660 None:intergenic
GCAAGTTTGCTAACTTCCTC+TGG 0.381827 4_2:+17287434 None:intergenic
TGAAGTTAAAGGAGTTATGA+TGG 0.406422 4_2:-17287390 Msa0592150:CDS
CCACATCTTTCTCCTCAATT+GGG 0.430467 4_2:+17286598 None:intergenic
GGCACAAGATTTCAGGCAGC+AGG 0.435566 4_2:-17286624 Msa0592150:CDS
ATTGTTCTTTCTGTGTGCCA+TGG 0.487473 4_2:-17286515 Msa0592150:CDS
AAAGTCTTAACAAAGAATTT+GGG 0.491739 4_2:-17287581 Msa0592150:intron
ATGCATTAGACCAAAGTTGA+AGG 0.514320 4_2:-17287483 Msa0592150:intron
TTTAATAAAAGATATGGTGG+AGG 0.542291 4_2:-17287627 Msa0592150:CDS
GGCAGCAGGGAACCCAATTG+AGG 0.564168 4_2:-17286610 Msa0592150:CDS
ATGGTTAACAACTGAAGCCA+TGG 0.597756 4_2:+17286498 None:intergenic
GCACAAGATTTCAGGCAGCA+GGG 0.625497 4_2:-17286623 Msa0592150:CDS
CCCAATTGAGGAGAAAGATG+TGG 0.650037 4_2:-17286598 Msa0592150:CDS
ATATTGTGTGGAACATCCAG+AGG 0.668552 4_2:-17287450 Msa0592150:CDS
GGAGCAAATGCAATATTGTG+TGG 0.673674 4_2:-17287462 Msa0592150:CDS
TATGATGGAAAATATCGACA+AGG 0.735916 4_2:-17287375 Msa0592150:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATGATTTTAATAAAAGATA+TGG - chr4_2:17286556-17286575 Msa0592150:CDS 15.0%
!! ATTAACATGTAAATATATGT+TGG + chr4_2:17286664-17286683 None:intergenic 15.0%
!! TTATATATGTAGATTGAATT+AGG - chr4_2:17287109-17287128 Msa0592150:intron 15.0%
!!! GATGATTTTAATAAAAGATA+TGG - chr4_2:17286556-17286575 Msa0592150:CDS 15.0%
!! ATTAACATGTAAATATATGT+TGG + chr4_2:17286664-17286683 None:intergenic 15.0%
!! TTATATATGTAGATTGAATT+AGG - chr4_2:17287109-17287128 Msa0592150:intron 15.0%
!!! GATTTTAATAAAAGATATGG+TGG - chr4_2:17286559-17286578 Msa0592150:CDS 20.0%
!! AAAAGTCTTAACAAAGAATT+TGG - chr4_2:17286607-17286626 Msa0592150:CDS 20.0%
!! AAAGTCTTAACAAAGAATTT+GGG - chr4_2:17286608-17286627 Msa0592150:CDS 20.0%
!! ATCTACATATATAAAACTGT+TGG + chr4_2:17287103-17287122 None:intergenic 20.0%
!!! CATCTAAAACTATAAAACAT+TGG + chr4_2:17287313-17287332 None:intergenic 20.0%
!!! CAATGTTTTATAGTTTTAGA+TGG - chr4_2:17287311-17287330 Msa0592150:intron 20.0%
!! GTTGTAGTTATTTATTATCT+AGG - chr4_2:17287457-17287476 Msa0592150:CDS 20.0%
!! TCCATTAAATTCTGAATTAT+TGG - chr4_2:17287510-17287529 Msa0592150:intron 20.0%
!! TCCAATAATTCAGAATTTAA+TGG + chr4_2:17287514-17287533 None:intergenic 20.0%
!!! GATTTTAATAAAAGATATGG+TGG - chr4_2:17286559-17286578 Msa0592150:CDS 20.0%
!! AAAAGTCTTAACAAAGAATT+TGG - chr4_2:17286607-17286626 Msa0592150:CDS 20.0%
!! AAAGTCTTAACAAAGAATTT+GGG - chr4_2:17286608-17286627 Msa0592150:CDS 20.0%
!! ATCTACATATATAAAACTGT+TGG + chr4_2:17287103-17287122 None:intergenic 20.0%
!!! CATCTAAAACTATAAAACAT+TGG + chr4_2:17287313-17287332 None:intergenic 20.0%
!!! CAATGTTTTATAGTTTTAGA+TGG - chr4_2:17287311-17287330 Msa0592150:intron 20.0%
!! GTTGTAGTTATTTATTATCT+AGG - chr4_2:17287457-17287476 Msa0592150:CDS 20.0%
!! TCCATTAAATTCTGAATTAT+TGG - chr4_2:17287510-17287529 Msa0592150:intron 20.0%
!! TCCAATAATTCAGAATTTAA+TGG + chr4_2:17287514-17287533 None:intergenic 20.0%
! TTTAATAAAAGATATGGTGG+AGG - chr4_2:17286562-17286581 Msa0592150:CDS 25.0%
!! CATATATAAAACTGTTGGTT+TGG + chr4_2:17287098-17287117 None:intergenic 25.0%
! ATGCATATAGTATTTAGATC+TGG - chr4_2:17287184-17287203 Msa0592150:intron 25.0%
! AAAACTAGATGTGAGAATTT+GGG + chr4_2:17287390-17287409 None:intergenic 25.0%
! TATTGGAATGTCAATTGAAA+TGG - chr4_2:17287527-17287546 Msa0592150:intron 25.0%
! TTTAATAAAAGATATGGTGG+AGG - chr4_2:17286562-17286581 Msa0592150:CDS 25.0%
!! CATATATAAAACTGTTGGTT+TGG + chr4_2:17287098-17287117 None:intergenic 25.0%
! ATGCATATAGTATTTAGATC+TGG - chr4_2:17287184-17287203 Msa0592150:intron 25.0%
! AAAACTAGATGTGAGAATTT+GGG + chr4_2:17287390-17287409 None:intergenic 25.0%
! TATTGGAATGTCAATTGAAA+TGG - chr4_2:17287527-17287546 Msa0592150:intron 25.0%
ATACGTTCAAGAAAAGCTAT+TGG + chr4_2:17286532-17286551 None:intergenic 30.0%
! AGCTTTTCTTGAACGTATTA+AGG - chr4_2:17286534-17286553 Msa0592150:CDS 30.0%
TGAAGTTAAAGGAGTTATGA+TGG - chr4_2:17286799-17286818 Msa0592150:intron 30.0%
! TATGATGGAAAATATCGACA+AGG - chr4_2:17286814-17286833 Msa0592150:intron 30.0%
! ATTTTCTTGGACAATCATAG+AGG + chr4_2:17286875-17286894 None:intergenic 30.0%
ATTGAATTAGGAGATGATTC+TGG - chr4_2:17287121-17287140 Msa0592150:intron 30.0%
!!! TATAGTTTTAGATGGAGTTG+TGG - chr4_2:17287319-17287338 Msa0592150:intron 30.0%
GAAAACTAGATGTGAGAATT+TGG + chr4_2:17287391-17287410 None:intergenic 30.0%
GAATGTCAATTGAAATGGTA+TGG - chr4_2:17287532-17287551 Msa0592150:intron 30.0%
ATACGTTCAAGAAAAGCTAT+TGG + chr4_2:17286532-17286551 None:intergenic 30.0%
! AGCTTTTCTTGAACGTATTA+AGG - chr4_2:17286534-17286553 Msa0592150:CDS 30.0%
TGAAGTTAAAGGAGTTATGA+TGG - chr4_2:17286799-17286818 Msa0592150:intron 30.0%
! TATGATGGAAAATATCGACA+AGG - chr4_2:17286814-17286833 Msa0592150:intron 30.0%
! ATTTTCTTGGACAATCATAG+AGG + chr4_2:17286875-17286894 None:intergenic 30.0%
ATTGAATTAGGAGATGATTC+TGG - chr4_2:17287121-17287140 Msa0592150:intron 30.0%
!!! TATAGTTTTAGATGGAGTTG+TGG - chr4_2:17287319-17287338 Msa0592150:intron 30.0%
GAAAACTAGATGTGAGAATT+TGG + chr4_2:17287391-17287410 None:intergenic 30.0%
GAATGTCAATTGAAATGGTA+TGG - chr4_2:17287532-17287551 Msa0592150:intron 30.0%
ATGCATTAGACCAAAGTTGA+AGG - chr4_2:17286706-17286725 Msa0592150:intron 35.0%
!! GCTCAAGTTTCTGAAGTTAA+AGG - chr4_2:17286788-17286807 Msa0592150:intron 35.0%
!!! AAGTGTCAGTGCTATTTTCT+TGG + chr4_2:17286888-17286907 None:intergenic 35.0%
CTTCACATTGAAGAACTGTT+CGG - chr4_2:17286905-17286924 Msa0592150:intron 35.0%
! TTATGTGTCCGTGTATGTTT+TGG - chr4_2:17286954-17286973 Msa0592150:intron 35.0%
! ATGAAGCACCAAAACATACA+CGG + chr4_2:17286965-17286984 None:intergenic 35.0%
! ATTGTCTCTAGAGAAGATTG+AGG - chr4_2:17286994-17287013 Msa0592150:intron 35.0%
! TCTAGAGAAGATTGAGGTTT+TGG - chr4_2:17287000-17287019 Msa0592150:intron 35.0%
ACTCGAATGATGTCTTTGTT+TGG - chr4_2:17287213-17287232 Msa0592150:intron 35.0%
! TTGTTTGGTTTAAGTGTGAG+TGG - chr4_2:17287228-17287247 Msa0592150:intron 35.0%
ATGCATTAGACCAAAGTTGA+AGG - chr4_2:17286706-17286725 Msa0592150:intron 35.0%
!! GCTCAAGTTTCTGAAGTTAA+AGG - chr4_2:17286788-17286807 Msa0592150:intron 35.0%
!!! AAGTGTCAGTGCTATTTTCT+TGG + chr4_2:17286888-17286907 None:intergenic 35.0%
CTTCACATTGAAGAACTGTT+CGG - chr4_2:17286905-17286924 Msa0592150:intron 35.0%
! TTATGTGTCCGTGTATGTTT+TGG - chr4_2:17286954-17286973 Msa0592150:intron 35.0%
! ATGAAGCACCAAAACATACA+CGG + chr4_2:17286965-17286984 None:intergenic 35.0%
! ATTGTCTCTAGAGAAGATTG+AGG - chr4_2:17286994-17287013 Msa0592150:intron 35.0%
! TCTAGAGAAGATTGAGGTTT+TGG - chr4_2:17287000-17287019 Msa0592150:intron 35.0%
ACTCGAATGATGTCTTTGTT+TGG - chr4_2:17287213-17287232 Msa0592150:intron 35.0%
! TTGTTTGGTTTAAGTGTGAG+TGG - chr4_2:17287228-17287247 Msa0592150:intron 35.0%
TGCATTTGCTCCTTCAACTT+TGG + chr4_2:17286719-17286738 None:intergenic 40.0%
GGAGCAAATGCAATATTGTG+TGG - chr4_2:17286727-17286746 Msa0592150:intron 40.0%
ATATTGTGTGGAACATCCAG+AGG - chr4_2:17286739-17286758 Msa0592150:intron 40.0%
GTTCGGTATCTACATGTCAT+TGG - chr4_2:17286922-17286941 Msa0592150:intron 40.0%
!! AGAGAAGATTGAGGTTTTGG+TGG - chr4_2:17287003-17287022 Msa0592150:intron 40.0%
GTTGTGGTGTCTAAATCTCT+TGG - chr4_2:17287335-17287354 Msa0592150:intron 40.0%
TTGATGCCTTAGAGATCACT+GGG - chr4_2:17287414-17287433 Msa0592150:CDS 40.0%
CCACATCTTTCTCCTCAATT+GGG + chr4_2:17287594-17287613 None:intergenic 40.0%
ACCACATCTTTCTCCTCAAT+TGG + chr4_2:17287595-17287614 None:intergenic 40.0%
ATTGTTCTTTCTGTGTGCCA+TGG - chr4_2:17287674-17287693 Msa0592150:CDS 40.0%
TGCATTTGCTCCTTCAACTT+TGG + chr4_2:17286719-17286738 None:intergenic 40.0%
GGAGCAAATGCAATATTGTG+TGG - chr4_2:17286727-17286746 Msa0592150:intron 40.0%
ATATTGTGTGGAACATCCAG+AGG - chr4_2:17286739-17286758 Msa0592150:intron 40.0%
GTTCGGTATCTACATGTCAT+TGG - chr4_2:17286922-17286941 Msa0592150:intron 40.0%
!! AGAGAAGATTGAGGTTTTGG+TGG - chr4_2:17287003-17287022 Msa0592150:intron 40.0%
GTTGTGGTGTCTAAATCTCT+TGG - chr4_2:17287335-17287354 Msa0592150:intron 40.0%
TTGATGCCTTAGAGATCACT+GGG - chr4_2:17287414-17287433 Msa0592150:CDS 40.0%
CCACATCTTTCTCCTCAATT+GGG + chr4_2:17287594-17287613 None:intergenic 40.0%
ACCACATCTTTCTCCTCAAT+TGG + chr4_2:17287595-17287614 None:intergenic 40.0%
ATTGTTCTTTCTGTGTGCCA+TGG - chr4_2:17287674-17287693 Msa0592150:CDS 40.0%
!! GCAAGTTTGCTAACTTCCTC+TGG + chr4_2:17286758-17286777 None:intergenic 45.0%
AGAATCTAACCTGAGAGCGA+AGG + chr4_2:17287042-17287061 None:intergenic 45.0%
GTTGATGCCTTAGAGATCAC+TGG - chr4_2:17287413-17287432 Msa0592150:CDS 45.0%
TCGAATCCCAGTGATCTCTA+AGG + chr4_2:17287423-17287442 None:intergenic 45.0%
! AAATGGTATGGCTTTGCTGC+AGG - chr4_2:17287544-17287563 Msa0592150:intron 45.0%
CCCAATTGAGGAGAAAGATG+TGG - chr4_2:17287591-17287610 Msa0592150:CDS 45.0%
!! GCAAGTTTGCTAACTTCCTC+TGG + chr4_2:17286758-17286777 None:intergenic 45.0%
AGAATCTAACCTGAGAGCGA+AGG + chr4_2:17287042-17287061 None:intergenic 45.0%
GTTGATGCCTTAGAGATCAC+TGG - chr4_2:17287413-17287432 Msa0592150:CDS 45.0%
TCGAATCCCAGTGATCTCTA+AGG + chr4_2:17287423-17287442 None:intergenic 45.0%
! AAATGGTATGGCTTTGCTGC+AGG - chr4_2:17287544-17287563 Msa0592150:intron 45.0%
CCCAATTGAGGAGAAAGATG+TGG - chr4_2:17287591-17287610 Msa0592150:CDS 45.0%
AACTGAGAACCTTCGCTCTC+AGG - chr4_2:17287030-17287049 Msa0592150:intron 50.0%
TGCTGCAGGCACAAGATTTC+AGG - chr4_2:17287558-17287577 Msa0592150:intron 50.0%
GCACAAGATTTCAGGCAGCA+GGG - chr4_2:17287566-17287585 Msa0592150:intron 50.0%
AACTGAGAACCTTCGCTCTC+AGG - chr4_2:17287030-17287049 Msa0592150:intron 50.0%
TGCTGCAGGCACAAGATTTC+AGG - chr4_2:17287558-17287577 Msa0592150:intron 50.0%
GCACAAGATTTCAGGCAGCA+GGG - chr4_2:17287566-17287585 Msa0592150:intron 50.0%
TGGGAGCAACTCGATGATCC+AGG + chr4_2:17287371-17287390 None:intergenic 55.0%
GGCACAAGATTTCAGGCAGC+AGG - chr4_2:17287565-17287584 Msa0592150:intron 55.0%
TGGGAGCAACTCGATGATCC+AGG + chr4_2:17287371-17287390 None:intergenic 55.0%
GGCACAAGATTTCAGGCAGC+AGG - chr4_2:17287565-17287584 Msa0592150:intron 55.0%
TCTCTTGGTCGTCGACGTCC+TGG - chr4_2:17287350-17287369 Msa0592150:intron 60.0%
GGCAGCAGGGAACCCAATTG+AGG - chr4_2:17287579-17287598 Msa0592150:intron 60.0%
TCTCTTGGTCGTCGACGTCC+TGG - chr4_2:17287350-17287369 Msa0592150:intron 60.0%
GGCAGCAGGGAACCCAATTG+AGG - chr4_2:17287579-17287598 Msa0592150:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 17286505 17287706 17286505 ID=Msa0592150;Name=Msa0592150
chr4_2 mRNA 17286505 17287706 17286505 ID=Msa0592150-mRNA-1;Parent=Msa0592150;Name=Msa0592150-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|0.33|3|0|129
chr4_2 exon 17287582 17287706 17287582 ID=Msa0592150-mRNA-1:exon:5019;Parent=Msa0592150-mRNA-1
chr4_2 exon 17287376 17287496 17287376 ID=Msa0592150-mRNA-1:exon:5018;Parent=Msa0592150-mRNA-1
chr4_2 exon 17286505 17286645 17286505 ID=Msa0592150-mRNA-1:exon:5017;Parent=Msa0592150-mRNA-1
chr4_2 CDS 17287582 17287706 17287582 ID=Msa0592150-mRNA-1:cds;Parent=Msa0592150-mRNA-1
chr4_2 CDS 17287376 17287496 17287376 ID=Msa0592150-mRNA-1:cds;Parent=Msa0592150-mRNA-1
chr4_2 CDS 17286505 17286645 17286505 ID=Msa0592150-mRNA-1:cds;Parent=Msa0592150-mRNA-1
Gene Sequence

>Msa0592150

GTGGAGTCTGTTGGTAGACAAATTCCAATAGCTTTTCTTGAACGTATTAAGGATGATTTTAATAAAAGATATGGTGGAGGAAGAGCTACTACAGCAACTGCTAAAAGTCTTAACAAAGAATTTGGACCAAAGTTGAAGGAGCAAATGCAATATTGTGTGGAACATCCAGAGGAAGTTAGCAAACTTGCTAAAGTGAAAGCTCAAGTTTCTGAAGTTAAAGGAGTTATGATGGAAAATATCGACAAGGCACAAGATTTCAGGCAGCAGGGAACCCAATTGAGGAGAAAGATGTGGTATCAAAACATGAAGATCAAACTCATAGTTCTTGCTATAATAATTGCATTGATTCTCATTATTGTTCTTTCTGTGTGCCATGGCTTCAGTTGT

Protein sequence

>Msa0592150

VESVGRQIPIAFLERIKDDFNKRYGGGRATTATAKSLNKEFGPKLKEQMQYCVEHPEEVSKLAKVKAQVSEVKGVMMENIDKAQDFRQQGTQLRRKMWYQNMKIKLIVLAIIIALILIIVLSVCHGFSC