AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0603530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603530 MtrunA17_Chr4g0032201 99.535 215 1 0 1 215 1 215 5.84e-161 443
Msa0603530 MtrunA17_Chr3g0112681 84.186 215 33 1 1 214 1 215 1.55e-133 374
Msa0603530 MtrunA17_Chr2g0276941 75.472 212 50 2 6 215 9 220 1.18e-117 333
Msa0603530 MtrunA17_Chr2g0315551 69.159 214 65 1 3 215 4 217 2.60e-110 315
Msa0603530 MtrunA17_Chr2g0283131 68.519 216 63 3 2 214 3 216 1.33e-105 303
Msa0603530 MtrunA17_Chr4g0046261 68.056 216 64 3 2 214 3 216 1.44e-105 303
Msa0603530 MtrunA17_Chr4g0040781 68.056 216 64 3 2 214 3 216 3.13e-105 302
Msa0603530 MtrunA17_Chr2g0319951 69.626 214 63 2 3 214 4 217 1.06e-104 301
Msa0603530 MtrunA17_Chr2g0325091 67.606 213 65 3 5 215 4 214 1.03e-101 293
Msa0603530 MtrunA17_Chr8g0381841 66.038 212 68 1 6 213 11 222 6.77e-98 284
Msa0603530 MtrunA17_Chr3g0123821 63.679 212 73 1 6 213 10 221 2.47e-96 280
Msa0603530 MtrunA17_Chr1g0174281 62.617 214 74 3 6 213 9 222 5.60e-93 271
Msa0603530 MtrunA17_Chr7g0231131 62.319 207 75 2 10 213 13 219 6.04e-91 266
Msa0603530 MtrunA17_Chr1g0189361 58.962 212 80 2 10 214 12 223 2.40e-88 259
Msa0603530 MtrunA17_Chr8g0346421 61.458 192 74 0 3 194 17 208 1.13e-85 253
Msa0603530 MtrunA17_Chr7g0214241 55.556 207 89 1 11 214 14 220 6.50e-84 248
Msa0603530 MtrunA17_Chr7g0234201 56.373 204 89 0 11 214 12 215 2.20e-81 241
Msa0603530 MtrunA17_Chr8g0351861 53.636 220 92 3 5 214 8 227 5.95e-81 241
Msa0603530 MtrunA17_Chr6g0482721 54.630 216 95 3 1 214 1 215 6.14e-81 240
Msa0603530 MtrunA17_Chr7g0275991 57.500 200 85 0 2 201 10 209 1.02e-80 240
Msa0603530 MtrunA17_Chr8g0392931 54.028 211 95 2 6 214 8 218 1.41e-74 224
Msa0603530 MtrunA17_Chr4g0055651 46.919 211 103 2 5 215 1 202 8.78e-67 204
Msa0603530 MtrunA17_Chr8g0335471 47.393 211 103 2 5 215 1 203 1.74e-66 203
Msa0603530 MtrunA17_Chr7g0250531 46.919 211 103 2 5 215 1 202 1.90e-66 203
Msa0603530 MtrunA17_Chr2g0290891 45.755 212 106 3 9 214 3 211 3.46e-61 190
Msa0603530 MtrunA17_Chr6g0458081 54.430 158 72 0 9 166 3 160 2.27e-60 188
Msa0603530 MtrunA17_Chr1g0212521 45.755 212 101 5 8 213 11 214 5.89e-60 187
Msa0603530 MtrunA17_Chr1g0182321 44.495 218 107 5 4 215 7 216 2.15e-59 186
Msa0603530 MtrunA17_Chr8g0349711 46.190 210 105 2 11 214 5 212 4.02e-59 185
Msa0603530 MtrunA17_Chr1g0182301 44.037 218 108 5 4 215 7 216 7.50e-59 184
Msa0603530 MtrunA17_Chr4g0030911 44.131 213 102 3 8 213 11 213 1.56e-58 183
Msa0603530 MtrunA17_Chr7g0263071 45.327 214 103 5 8 215 11 216 4.42e-58 182
Msa0603530 MtrunA17_Chr2g0276931 46.316 190 101 1 7 195 4 193 1.84e-57 180
Msa0603530 MtrunA17_Chr8g0349681 44.928 207 110 2 9 214 381 584 1.32e-54 183
Msa0603530 MtrunA17_Chr8g0349681 52.201 159 76 0 11 169 12 170 1.38e-50 172
Msa0603530 MtrunA17_Chr8g0349681 50.000 166 83 0 9 174 172 337 1.04e-49 170
Msa0603530 MtrunA17_Chr2g0327911 47.059 170 88 2 6 173 9 178 1.76e-53 171
Msa0603530 MtrunA17_Chr3g0109721 42.781 187 99 2 14 197 12 193 2.28e-49 159
Msa0603530 MtrunA17_Chr5g0433561 42.781 187 99 2 14 197 12 193 4.24e-49 159
Msa0603530 MtrunA17_Chr1g0199331 48.408 157 80 1 14 169 33 189 3.85e-48 156
Msa0603530 MtrunA17_Chr8g0384311 37.799 209 120 5 7 213 8 208 1.64e-47 155
Msa0603530 MtrunA17_Chr7g0265001 43.269 208 110 4 9 214 13 214 2.91e-47 154
Msa0603530 MtrunA17_Chr8g0382221 36.408 206 118 3 13 214 10 206 1.83e-45 150
Msa0603530 MtrunA17_Chr3g0123971 41.401 157 92 0 13 169 10 166 2.33e-45 149
Msa0603530 MtrunA17_Chr8g0390561 36.453 203 112 3 14 214 11 198 3.38e-45 149
Msa0603530 MtrunA17_Chr1g0159481 38.278 209 120 5 8 214 13 214 4.41e-45 149
Msa0603530 MtrunA17_Chr1g0180291 45.349 172 91 3 9 178 11 181 6.39e-45 149
Msa0603530 MtrunA17_Chr5g0400131 36.893 206 117 2 13 214 10 206 6.92e-45 148
Msa0603530 MtrunA17_Chr4g0034871 38.462 208 110 5 12 212 8 204 3.13e-43 144
Msa0603530 MtrunA17_Chr4g0055081 39.490 157 95 0 13 169 10 166 4.53e-43 144
Msa0603530 MtrunA17_Chr3g0130271 37.788 217 122 8 1 213 1 208 5.58e-43 143
Msa0603530 MtrunA17_Chr1g0197331 43.353 173 95 3 9 178 12 184 3.03e-42 142
Msa0603530 MtrunA17_Chr2g0331691 40.000 165 92 3 12 169 10 174 3.58e-38 131
Msa0603530 MtrunA17_Chr4g0004661 35.885 209 116 5 12 213 64 261 8.32e-38 132
Msa0603530 MtrunA17_Chr1g0201121 34.615 208 119 5 12 212 8 205 7.00e-36 125
Msa0603530 MtrunA17_Chr8g0383261 36.471 170 101 2 7 169 7 176 7.79e-36 125
Msa0603530 MtrunA17_Chr6g0466301 37.576 165 96 3 12 169 8 172 1.18e-35 124
Msa0603530 MtrunA17_Chr6g0465491 37.576 165 96 3 12 169 8 172 1.18e-35 124
Msa0603530 MtrunA17_Chr3g0138121 31.667 180 115 3 13 187 14 190 4.98e-25 97.8
Msa0603530 MtrunA17_Chr3g0138101 31.667 180 115 3 13 187 14 190 5.71e-25 97.4
Msa0603530 MtrunA17_Chr3g0138111 31.667 180 115 3 13 187 14 190 5.77e-25 97.4
Msa0603530 MtrunA17_Chr6g0466161 34.211 152 89 4 27 169 5 154 2.04e-24 95.9
Msa0603530 MtrunA17_Chr6g0465471 34.211 152 89 4 27 169 5 154 2.25e-24 95.9
Msa0603530 MtrunA17_Chr6g0486041 29.293 198 123 6 12 194 6 201 1.78e-20 85.5
Msa0603530 MtrunA17_Chr4g0046211 33.103 145 87 4 12 155 6 141 2.99e-20 83.2
Msa0603530 MtrunA17_Chr2g0325101 31.737 167 100 5 12 166 6 170 6.22e-20 83.6
Msa0603530 MtrunA17_Chr4g0037021 31.757 148 91 4 12 158 6 144 6.08e-19 81.3
Msa0603530 MtrunA17_Chr3g0117931 30.539 167 102 5 12 166 8 172 1.12e-18 80.9
Msa0603530 MtrunA17_Chr8g0371501 32.609 138 83 4 12 148 6 134 4.06e-18 79.0
Msa0603530 MtrunA17_Chr6g0466431 35.922 103 57 1 12 114 8 101 8.95e-18 75.5
Msa0603530 MtrunA17_Chr6g0465611 35.922 103 57 1 12 114 8 101 8.95e-18 75.5
Msa0603530 MtrunA17_Chr5g0405791 31.884 138 84 4 12 148 6 134 6.05e-17 75.9
Msa0603530 MtrunA17_Chr1g0178291 30.909 165 104 4 9 173 428 582 4.23e-16 76.3
Msa0603530 MtrunA17_Chr5g0443631 28.824 170 113 2 13 176 105 272 7.83e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0603530 AT1G07410.1 90.233 215 20 1 1 215 1 214 1.71e-146 406
Msa0603530 AT3G46830.1 86.175 217 28 1 1 215 1 217 1.56e-136 381
Msa0603530 AT3G46830.2 86.175 217 28 1 1 215 1 217 1.56e-136 381
Msa0603530 AT5G59150.1 85.714 217 29 1 1 215 1 217 2.44e-136 381
Msa0603530 AT1G09630.1 74.537 216 54 1 1 215 1 216 8.97e-122 344
Msa0603530 AT4G18800.1 70.283 212 61 2 3 214 4 213 1.57e-109 313
Msa0603530 AT1G16920.1 70.093 214 62 2 2 214 3 215 1.71e-108 310
Msa0603530 AT5G45750.1 68.545 213 65 2 3 214 4 215 5.22e-107 306
Msa0603530 AT5G60860.1 69.444 216 61 3 2 214 3 216 2.12e-106 305
Msa0603530 AT1G06400.1 67.290 214 68 2 2 214 3 215 3.49e-104 300
Msa0603530 AT1G28550.1 65.278 216 71 2 2 214 3 217 3.79e-102 295
Msa0603530 AT3G15060.1 66.359 217 68 3 2 215 3 217 4.67e-102 294
Msa0603530 AT2G33870.1 65.278 216 71 2 3 215 4 218 4.88e-102 294
Msa0603530 AT4G18430.1 64.352 216 72 3 2 214 3 216 2.44e-101 292
Msa0603530 AT5G47960.1 64.019 214 73 2 6 215 9 222 2.79e-97 282
Msa0603530 AT5G59150.2 82.927 164 26 1 54 215 3 166 1.06e-95 276
Msa0603530 AT3G12160.1 63.507 211 74 2 6 213 9 219 2.71e-94 275
Msa0603530 AT5G65270.1 61.395 215 77 2 7 215 12 226 5.46e-93 271
Msa0603530 AT4G39990.1 59.535 215 72 3 10 215 15 223 2.69e-90 265
Msa0603530 AT5G47520.1 57.944 214 86 2 6 215 8 221 1.09e-87 258
Msa0603530 AT3G07410.1 56.019 216 93 1 1 214 1 216 4.18e-83 246
Msa0603530 AT1G05810.1 55.769 208 90 1 10 215 10 217 1.38e-82 245
Msa0603530 AT1G05810.2 55.769 208 90 1 10 215 56 263 3.66e-82 246
Msa0603530 AT1G73640.1 56.522 207 85 1 6 207 7 213 4.49e-82 244
Msa0603530 AT2G31680.1 55.238 210 89 2 10 215 10 218 3.28e-81 241
Msa0603530 AT2G43130.1 62.428 173 65 0 10 182 10 182 2.59e-79 236
Msa0603530 AT1G18200.1 52.489 221 92 2 6 213 7 227 1.14e-78 235
Msa0603530 AT1G01200.1 57.789 199 79 3 10 205 26 222 9.91e-76 228
Msa0603530 AT1G02130.1 46.919 211 104 2 5 215 1 203 3.69e-67 205
Msa0603530 AT5G47200.1 46.919 211 103 2 5 215 1 202 1.59e-66 204
Msa0603530 AT4G17530.1 46.445 211 104 2 5 215 1 202 7.91e-66 202
Msa0603530 AT4G35860.1 48.325 209 103 3 9 213 3 210 8.15e-64 197
Msa0603530 AT3G11730.1 46.411 209 106 2 5 213 1 203 5.51e-62 192
Msa0603530 AT4G17170.1 45.972 211 105 3 9 213 3 210 3.16e-61 191
Msa0603530 AT3G53610.2 45.327 214 103 4 8 215 11 216 6.12e-60 187
Msa0603530 AT3G53610.3 45.327 214 103 4 8 215 11 216 6.12e-60 187
Msa0603530 AT3G53610.1 45.327 214 103 4 8 215 11 216 6.12e-60 187
Msa0603530 AT5G59840.1 44.550 211 109 3 8 215 11 216 1.14e-58 184
Msa0603530 AT5G03520.1 45.283 212 111 3 4 214 7 214 1.33e-58 184
Msa0603530 AT3G09900.1 44.340 212 115 3 4 214 7 216 2.31e-58 183
Msa0603530 AT3G46060.2 44.498 209 112 2 8 215 11 216 3.35e-58 183
Msa0603530 AT3G46060.3 44.498 209 112 2 8 215 11 216 3.35e-58 183
Msa0603530 AT3G46060.1 44.498 209 112 2 8 215 11 216 3.35e-58 183
Msa0603530 AT4G17160.1 44.390 205 111 2 9 213 3 204 7.40e-58 182
Msa0603530 AT3G54840.1 49.682 157 78 1 14 169 35 191 8.90e-50 161
Msa0603530 AT3G54840.3 49.359 156 78 1 15 169 2 157 1.65e-49 159
Msa0603530 AT4G19640.1 39.303 201 110 2 14 214 12 200 2.77e-49 160
Msa0603530 AT3G54840.2 49.677 155 77 1 14 167 35 189 3.35e-49 159
Msa0603530 AT5G45130.1 40.594 202 106 4 14 214 12 200 7.02e-48 156
Msa0603530 AT1G43890.3 43.548 186 99 4 7 190 8 189 4.18e-47 154
Msa0603530 AT1G43890.1 43.548 186 99 4 7 190 8 189 4.18e-47 154
Msa0603530 AT1G43890.2 43.548 186 99 4 7 190 8 189 4.18e-47 154
Msa0603530 AT5G03530.2 42.788 208 110 5 9 214 10 210 8.60e-47 154
Msa0603530 AT5G03530.1 42.788 208 110 5 9 214 10 210 8.60e-47 154
Msa0603530 AT5G45130.2 40.500 200 105 4 16 214 22 208 1.06e-46 153
Msa0603530 AT4G35860.2 46.914 162 81 3 56 213 4 164 2.78e-45 148
Msa0603530 AT4G39890.1 41.772 158 91 1 13 169 10 167 1.84e-44 148
Msa0603530 AT5G03520.2 43.195 169 91 3 47 214 40 204 6.51e-44 146
Msa0603530 AT5G10260.1 34.951 206 121 2 13 214 10 206 7.98e-43 143
Msa0603530 AT2G44610.1 34.783 207 121 2 13 214 10 207 2.73e-42 142
Msa0603530 AT2G22290.1 38.854 157 96 0 13 169 10 166 6.71e-42 141
Msa0603530 AT3G18820.1 37.500 208 112 5 12 212 8 204 5.42e-41 139
Msa0603530 AT1G52280.1 36.058 208 115 5 12 212 8 204 6.09e-40 136
Msa0603530 AT5G64990.1 38.854 157 96 0 13 169 8 164 2.40e-39 134
Msa0603530 AT4G19640.2 44.615 130 72 0 14 143 12 141 1.30e-38 130
Msa0603530 AT4G19640.3 38.650 163 98 2 14 176 12 172 1.68e-38 132
Msa0603530 AT1G49300.2 36.058 208 115 5 12 212 8 204 1.27e-37 130
Msa0603530 AT1G49300.1 36.058 208 115 5 12 212 8 204 1.27e-37 130
Msa0603530 AT3G09910.3 42.442 172 96 3 9 178 10 180 2.83e-37 129
Msa0603530 AT3G09910.1 42.442 172 96 3 9 178 10 180 2.83e-37 129
Msa0603530 AT3G09910.2 42.442 172 96 3 9 178 10 180 2.83e-37 129
Msa0603530 AT3G16100.1 34.135 208 119 5 12 212 8 204 5.73e-37 128
Msa0603530 AT5G64990.2 37.195 164 96 1 13 169 8 171 1.49e-36 127
Msa0603530 AT3G09910.5 42.515 167 93 3 9 173 10 175 6.61e-36 125
Msa0603530 AT3G09910.4 42.515 167 93 3 9 173 10 175 6.61e-36 125
Msa0603530 AT1G22740.1 37.576 165 96 3 12 169 8 172 1.05e-35 125
Msa0603530 AT2G21880.1 36.970 165 97 3 12 169 9 173 5.30e-35 123
Msa0603530 AT4G09720.1 37.576 165 96 3 12 169 8 172 7.82e-35 123
Msa0603530 AT4G09720.4 36.471 170 96 3 12 169 8 177 7.48e-34 120
Msa0603530 AT5G46025.1 52.830 106 49 1 74 178 6 111 2.41e-33 116
Msa0603530 AT4G09720.3 35.227 176 96 3 12 169 8 183 7.45e-33 118
Msa0603530 AT5G39620.1 35.976 164 98 3 13 169 6 169 6.46e-30 110
Msa0603530 AT4G08190.1 78.125 64 11 1 103 166 67 127 3.08e-28 103
Msa0603530 AT2G21880.4 35.135 148 89 3 29 169 1 148 1.53e-26 101
Msa0603530 AT2G21880.3 35.135 148 89 3 29 169 1 148 1.53e-26 101
Msa0603530 AT5G55080.1 34.416 154 98 3 13 166 14 164 2.05e-26 101
Msa0603530 AT2G21880.2 32.121 165 97 4 12 169 9 165 3.23e-25 98.2
Msa0603530 AT5G20010.1 30.500 200 121 4 13 207 14 200 4.75e-25 98.2
Msa0603530 AT5G20020.1 31.667 180 115 3 13 187 14 190 7.56e-25 97.8
Msa0603530 AT5G55190.1 31.844 179 114 3 13 186 14 189 8.14e-25 97.4
Msa0603530 AT4G09720.6 37.681 138 79 3 39 169 1 138 1.20e-24 95.9
Msa0603530 AT4G09720.5 37.681 138 79 3 39 169 1 138 1.20e-24 95.9
Msa0603530 AT4G09720.2 37.681 138 79 3 39 169 1 138 1.20e-24 95.9
Msa0603530 AT2G44690.1 33.333 180 106 6 12 179 18 195 2.29e-24 96.3
Msa0603530 AT4G28950.1 28.571 182 118 5 12 183 6 185 2.38e-20 85.5
Msa0603530 AT4G35020.1 33.793 145 86 4 12 155 6 141 2.85e-20 85.1
Msa0603530 AT4G35020.2 33.793 145 86 4 12 155 6 141 2.85e-20 85.1
Msa0603530 AT4G35020.3 33.793 145 86 4 12 155 6 141 2.85e-20 85.1
Msa0603530 AT3G48040.1 29.208 202 125 6 12 201 8 203 5.23e-20 84.7
Msa0603530 AT3G48040.2 34.483 145 85 4 12 155 8 143 1.21e-19 82.8
Msa0603530 AT5G62880.2 33.793 145 86 4 12 155 8 143 3.60e-19 82.0
Msa0603530 AT5G45970.1 30.682 176 94 5 12 168 6 172 4.03e-19 82.0
Msa0603530 AT5G62880.1 29.282 181 100 5 12 173 8 179 4.14e-19 82.4
Msa0603530 AT1G75840.1 33.333 138 82 4 12 148 6 134 2.68e-18 79.7
Msa0603530 AT1G20090.1 32.609 138 83 4 12 148 5 133 4.75e-18 79.0
Msa0603530 AT3G51300.1 30.909 165 100 6 14 166 8 170 1.13e-17 78.2
Msa0603530 AT4G35950.1 32.609 138 83 4 12 148 6 134 1.49e-17 77.8
Msa0603530 AT2G17800.2 32.609 138 83 4 12 148 6 134 1.62e-17 77.8
Msa0603530 AT2G17800.1 32.609 138 83 4 12 148 6 134 1.62e-17 77.8
Msa0603530 AT4G35020.4 35.088 114 71 3 14 126 8 119 1.03e-16 74.3
Msa0603530 AT4G35020.5 35.088 114 71 3 14 126 8 119 1.03e-16 74.3

Find 93 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGGTGGCTGCGTGAATTA+AGG 0.194045 4_2:-48120827 Msa0603530:CDS
ACTAGGGTTGCTAGTTTCTT+TGG 0.249793 4_2:-48120325 Msa0603530:three_prime_UTR
TAATGAGAATCTGACGTTTC+AGG 0.250516 4_2:-48120221 Msa0603530:three_prime_UTR
GCAGCTGGTGCCTCACTTCC+TGG 0.264579 4_2:-48120579 Msa0603530:CDS
GAAGTTACCGTGAGAAGAAT+TGG 0.327345 4_2:+48122228 None:intergenic
GACTGTAAAGGCACAGATTT+GGG 0.346030 4_2:-48120961 Msa0603530:CDS
GAACCATCTTAGAGCTGTTT+CGG 0.347178 4_2:-48120751 Msa0603530:CDS
ACCAACAGCTCCTCTGTAAT+AGG 0.370535 4_2:+48120899 None:intergenic
TTGGAGTCTAAATCTACTAT+TGG 0.376344 4_2:-48121951 Msa0603530:CDS
ATGATAAATCTCTGTCAATA+TGG 0.378805 4_2:+48120635 None:intergenic
TGGAAGTACCTGAAGGGTTC+TGG 0.388708 4_2:+48121914 None:intergenic
TGAGAAGAATTGGATTGATT+TGG 0.389781 4_2:+48122238 None:intergenic
CCGGTGGCTGCGTGAATTAA+GGG 0.390640 4_2:-48120826 Msa0603530:CDS
GTAAGATTAAAATCTGCTTT+CGG 0.391275 4_2:-48120190 Msa0603530:three_prime_UTR
ATTCGCCACCAGAACCCTTC+AGG 0.400128 4_2:-48121922 Msa0603530:intron
GATATTTGATGATATAGCTT+TGG 0.405771 4_2:-48120245 Msa0603530:three_prime_UTR
AGACTGTAAAGGCACAGATT+TGG 0.408612 4_2:-48120962 Msa0603530:CDS
GAATGTACGGTCATCCCCTT+TGG 0.409242 4_2:-48120121 Msa0603530:three_prime_UTR
GAGTGTGATTTGAAACGGTT+CGG 0.411082 4_2:+48122302 None:intergenic
TGTTTATCTCTCACCCATAA+AGG 0.415378 4_2:+48120405 None:intergenic
CAACATTGATGGTGGTTCCT+TGG 0.417789 4_2:+48120556 None:intergenic
ATCGGAGAGGAGAAGATCTA+TGG 0.418807 4_2:-48122084 Msa0603530:exon
CTTGCAGCTCAGGAAGCAGC+TGG 0.422553 4_2:-48120594 Msa0603530:CDS
AATGAGAATCTGACGTTTCA+GGG 0.426836 4_2:-48120220 Msa0603530:three_prime_UTR
AGTGTGATTTGAAACGGTTC+GGG 0.431406 4_2:+48122303 None:intergenic
ACAGCAGCCTCTCTTTGTAT+TGG 0.436355 4_2:+48120521 None:intergenic
GTAAATCCAACATTCTCTCT+AGG 0.462112 4_2:-48121995 Msa0603530:CDS
AAATCTCTGTCAATATGGTC+TGG 0.463166 4_2:+48120640 None:intergenic
CCCTTAATTCACGCAGCCAC+CGG 0.470934 4_2:+48120826 None:intergenic
ATTGTGAAAGATTCAAAACT+AGG 0.470944 4_2:-48120342 Msa0603530:three_prime_UTR
GATTTGGGACACAGCCGGTC+AGG 0.472434 4_2:-48120946 Msa0603530:CDS
TGAGAGAGTGTGATTTGAAA+CGG 0.475401 4_2:+48122297 None:intergenic
TATATATATTCCATTCCAAA+GGG 0.479940 4_2:+48120106 None:intergenic
CTGGAATGCCTTCTCAATGT+TGG 0.480200 4_2:+48120659 None:intergenic
TTGATCGGAGATTCTGGTGT+TGG 0.486858 4_2:-48122017 Msa0603530:CDS
AAAGGGGATGACCGTACATT+CGG 0.489488 4_2:+48120123 None:intergenic
AAATGGCCAATTGGAGAAAA+CGG 0.492482 4_2:+48122394 None:intergenic
GAGAAGATCTATGGCGCATA+GGG 0.496739 4_2:-48122075 Msa0603530:exon
TTGATGGTGGTTCCTTGGCC+AGG 0.497262 4_2:+48120561 None:intergenic
TTGTTTATTAAATTGTTACA+AGG 0.501338 4_2:-48120164 Msa0603530:three_prime_UTR
CTGTGTATGCAGCTGGATGG+TGG 0.503123 4_2:+48122583 None:intergenic
TTGTATATGACATTACTAAG+AGG 0.510206 4_2:-48120869 Msa0603530:CDS
CCACCGGTTGACATTGTCAA+AGG 0.511005 4_2:+48120842 None:intergenic
CGGTCAAGCATTGGCAGAGA+AGG 0.513673 4_2:-48120721 Msa0603530:CDS
CCTCCGAAACAGCTCTAAGA+TGG 0.521686 4_2:+48120748 None:intergenic
GGTTCCTTGGCCAGGAAGTG+AGG 0.525283 4_2:+48120569 None:intergenic
GGAGGATGACGGTCAAGCAT+TGG 0.525571 4_2:-48120730 Msa0603530:CDS
AGATGAATCTGCAACATTGA+TGG 0.526989 4_2:+48120545 None:intergenic
AAGGTGACTGTGTATGCAGC+TGG 0.529939 4_2:+48122576 None:intergenic
AAACCTTTGACAATGTCAAC+CGG 0.533316 4_2:-48120845 Msa0603530:CDS
GCCTATTACAGAGGAGCTGT+TGG 0.537767 4_2:-48120900 Msa0603530:CDS
AGCTCCTCTGTAATAGGCAC+TGG 0.538985 4_2:+48120905 None:intergenic
TACGGTCATCCCCTTTGGAA+TGG 0.541172 4_2:-48120116 Msa0603530:three_prime_UTR
TGTAAACCTAGAGAGAATGT+TGG 0.541295 4_2:+48121989 None:intergenic
TCTGATAGGTTGTTAGAGAT+CGG 0.541774 4_2:-48122102 Msa0603530:intron
ATATATATATTCCATTCCAA+AGG 0.542582 4_2:+48120105 None:intergenic
AACATAGTGATCATGATGGC+AGG 0.549455 4_2:-48120789 Msa0603530:CDS
AAAGAAGAAGAGGTAGAAGG+TGG 0.550026 4_2:+48122346 None:intergenic
GAGTATAGGACAGAAGATGA+AGG 0.551618 4_2:+48122557 None:intergenic
GGTGCCTCACTTCCTGGCCA+AGG 0.553261 4_2:-48120573 Msa0603530:CDS
AAGATCTATGGCGCATAGGG+TGG 0.554795 4_2:-48122072 Msa0603530:exon
ACGGTGTCGTATTAGAGTAT+AGG 0.558144 4_2:+48122543 None:intergenic
GGAGAAGATCTATGGCGCAT+AGG 0.558633 4_2:-48122076 Msa0603530:exon
TTGTGAAAGATTCAAAACTA+GGG 0.562735 4_2:-48120341 Msa0603530:three_prime_UTR
TGTGTATGCAGCTGGATGGT+GGG 0.563495 4_2:+48122584 None:intergenic
CAAAAGAAACTATCACATCA+AGG 0.566272 4_2:+48120293 None:intergenic
TGCACGGTACCGCTCCTGAC+CGG 0.575514 4_2:+48120932 None:intergenic
GCACAGATTTGGGACACAGC+CGG 0.589077 4_2:-48120951 Msa0603530:CDS
AATCAATCCAATTCTTCTCA+CGG 0.593636 4_2:-48122235 Msa0603530:intron
TGTCTTCCTCCACAGGTAGA+GGG 0.596147 4_2:-48120987 Msa0603530:intron
AGAGCTGTTTCGGAGGATGA+CGG 0.603075 4_2:-48120741 Msa0603530:CDS
TCATCTGCCAATACAAAGAG+AGG 0.610381 4_2:-48120528 Msa0603530:CDS
ATTACCAGTGCCTATTACAG+AGG 0.615988 4_2:-48120909 Msa0603530:CDS
CCATCTTAGAGCTGTTTCGG+AGG 0.619143 4_2:-48120748 Msa0603530:CDS
AGAAAATGGAAGTACCTGAA+GGG 0.622103 4_2:+48121908 None:intergenic
CAAGCATTGGCAGAGAAGGA+AGG 0.624298 4_2:-48120717 Msa0603530:CDS
GGAAGCAACCAACATTGAGA+AGG 0.627086 4_2:-48120667 Msa0603530:CDS
AATAGGCACTGGTAATTGCA+CGG 0.632997 4_2:+48120916 None:intergenic
GGTAGAGGGAAAGACTGTAA+AGG 0.636965 4_2:-48120973 Msa0603530:CDS
GGGACACAGCCGGTCAGGAG+CGG 0.638848 4_2:-48120941 Msa0603530:CDS
TTCTAACATAGTGATCATGA+TGG 0.639950 4_2:-48120793 Msa0603530:CDS
TACTTTGTTGAGTTGCGATG+AGG 0.647535 4_2:-48120368 Msa0603530:three_prime_UTR
TCACTATGTTAGAATCCGCA+TGG 0.651563 4_2:+48120802 None:intergenic
TAGGTTGTTAGAGATCGGAG+AGG 0.655671 4_2:-48122097 Msa0603530:five_prime_UTR
TGACTGTGTATGCAGCTGGA+TGG 0.661307 4_2:+48122580 None:intergenic
TGGTGGGTGAATGAAGTACA+AGG 0.665993 4_2:+48122600 None:intergenic
CCTTTGACAATGTCAACCGG+TGG 0.673657 4_2:-48120842 Msa0603530:CDS
AAGTACCTGAAGGGTTCTGG+TGG 0.684017 4_2:+48121917 None:intergenic
GCGTGAATTAAGGGACCATG+CGG 0.710292 4_2:-48120817 Msa0603530:CDS
TGAATCTGCAACATTGATGG+TGG 0.711567 4_2:+48120548 None:intergenic
GAGTGAGAAAGCACTTACAA+CGG 0.716201 4_2:+48122427 None:intergenic
ATATATATTCCATTCCAAAG+GGG 0.718919 4_2:+48120107 None:intergenic
GAGAATCTGACGTTTCAGGG+TGG 0.741050 4_2:-48120217 Msa0603530:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTGTTTTTTTTTTTATAT+TGG + chr4_2:48120195-48120214 None:intergenic 10.0%
!! AAAAAAATACTTAAATAACT+TGG + chr4_2:48120915-48120934 None:intergenic 10.0%
!!! AAGTATTTTTTTTTCATTTT+TGG - chr4_2:48120923-48120942 Msa0603530:CDS 10.0%
!!! TTTTTTTATATTGGAGAAAA+CGG + chr4_2:48120186-48120205 None:intergenic 15.0%
!!! GTTCGTAATATTTTTTAAAA+CGG + chr4_2:48121539-48121558 None:intergenic 15.0%
!! TTGTTTATTAAATTGTTACA+AGG - chr4_2:48122543-48122562 Msa0603530:five_prime_UTR 15.0%
!!! GAATGGAATATATATATTTT+TGG - chr4_2:48122608-48122627 Msa0603530:five_prime_UTR 15.0%
!! CTTAAATAACTTGGAAAATT+AGG + chr4_2:48120906-48120925 None:intergenic 20.0%
!!! TCTTTTTATTCTTCCTTTAT+GGG - chr4_2:48122289-48122308 Msa0603530:five_prime_UTR 20.0%
!! TATATATATTCCATTCCAAA+GGG + chr4_2:48122604-48122623 None:intergenic 20.0%
!! ATATATATATTCCATTCCAA+AGG + chr4_2:48122605-48122624 None:intergenic 20.0%
!!! ACCTCTTCTTCTTTTTTTTA+CGG - chr4_2:48120370-48120389 Msa0603530:three_prime_UTR 25.0%
! AACAGATCTAATAACTGAAT+CGG + chr4_2:48120514-48120533 None:intergenic 25.0%
!!! AGATCTGTTTCTTCATTTTT+TGG - chr4_2:48120525-48120544 Msa0603530:CDS 25.0%
!! ATCTGAATGAATTTTGTGAT+TGG - chr4_2:48120581-48120600 Msa0603530:CDS 25.0%
!!! TACAAGAAATGAGTTTTGTT+TGG - chr4_2:48120737-48120756 Msa0603530:CDS 25.0%
! GGAAAATTAGGATTATTACA+AGG + chr4_2:48120894-48120913 None:intergenic 25.0%
!! TTGTTTGAATTGATGGTATA+AGG - chr4_2:48121304-48121323 Msa0603530:intron 25.0%
!!! GTTTTAAGTTATAACAACAG+AGG + chr4_2:48121569-48121588 None:intergenic 25.0%
!! AAATTTTCTTTTGCTTGTGT+TGG - chr4_2:48121619-48121638 Msa0603530:intron 25.0%
! GTAACATTGTAATACAAACA+AGG + chr4_2:48121685-48121704 None:intergenic 25.0%
! TTGTATTACAATGTTACCAA+TGG - chr4_2:48121688-48121707 Msa0603530:intron 25.0%
! TTGTATATGACATTACTAAG+AGG - chr4_2:48121838-48121857 Msa0603530:intron 25.0%
! ATGATAAATCTCTGTCAATA+TGG + chr4_2:48122075-48122094 None:intergenic 25.0%
! AAGGAAGAATAAAAAGAGAA+AGG + chr4_2:48122286-48122305 None:intergenic 25.0%
!! CTCTTTTTATTCTTCCTTTA+TGG - chr4_2:48122288-48122307 Msa0603530:five_prime_UTR 25.0%
! ATTGTGAAAGATTCAAAACT+AGG - chr4_2:48122365-48122384 Msa0603530:five_prime_UTR 25.0%
! TTGTGAAAGATTCAAAACTA+GGG - chr4_2:48122366-48122385 Msa0603530:five_prime_UTR 25.0%
!! CTTTTGAATCGATGTAATAT+TGG - chr4_2:48122431-48122450 Msa0603530:five_prime_UTR 25.0%
!! GATATTTGATGATATAGCTT+TGG - chr4_2:48122462-48122481 Msa0603530:five_prime_UTR 25.0%
! GTAAGATTAAAATCTGCTTT+CGG - chr4_2:48122517-48122536 Msa0603530:five_prime_UTR 25.0%
! ATATATATTCCATTCCAAAG+GGG + chr4_2:48122603-48122622 None:intergenic 25.0%
! ATATTTCCGTTTTCTCCAAT+TGG - chr4_2:48120307-48120326 Msa0603530:three_prime_UTR 30.0%
!!! TGGAGTTTTTTTTTCAGAGA+GGG + chr4_2:48120344-48120363 None:intergenic 30.0%
AAAAAAGAAGAAGAGGTAGA+AGG + chr4_2:48120367-48120386 None:intergenic 30.0%
ACCGTAAAAAAAAGAAGAAG+AGG + chr4_2:48120374-48120393 None:intergenic 30.0%
!! ATTTGGTGAATTTTCTTCAG+TGG + chr4_2:48120455-48120474 None:intergenic 30.0%
TGAGAAGAATTGGATTGATT+TGG + chr4_2:48120472-48120491 None:intergenic 30.0%
AATCAATCCAATTCTTCTCA+CGG - chr4_2:48120472-48120491 Msa0603530:three_prime_UTR 30.0%
ATCTAATAACTGAATCGGAA+AGG + chr4_2:48120509-48120528 None:intergenic 30.0%
! ATTTTGTGATTGGATCTGAT+AGG - chr4_2:48120591-48120610 Msa0603530:CDS 30.0%
!! TTGTTCAAGATCGTTTTGAT+CGG - chr4_2:48120675-48120694 Msa0603530:CDS 30.0%
TTGGAGTCTAAATCTACTAT+TGG - chr4_2:48120756-48120775 Msa0603530:CDS 30.0%
AGAAATCAACAGAGAGAAAA+TGG + chr4_2:48120816-48120835 None:intergenic 30.0%
TACAAATACACGCATTCAAA+GGG + chr4_2:48120847-48120866 None:intergenic 30.0%
TTACAAATACACGCATTCAA+AGG + chr4_2:48120848-48120867 None:intergenic 30.0%
GGATTATTACAAGGATCAAT+TGG + chr4_2:48120885-48120904 None:intergenic 30.0%
! CGAGGTGAATGTAATTTTAT+TGG - chr4_2:48121256-48121275 Msa0603530:intron 30.0%
! TTTGAATTGATGGTATAAGG+TGG - chr4_2:48121307-48121326 Msa0603530:intron 30.0%
TGATATATGGTGTAATGAAG+TGG + chr4_2:48121467-48121486 None:intergenic 30.0%
TTCTAACATAGTGATCATGA+TGG - chr4_2:48121914-48121933 Msa0603530:intron 30.0%
AAAGGAGCAAAAACAGAATT+GGG + chr4_2:48122268-48122287 None:intergenic 30.0%
CAAAAGAAACTATCACATCA+AGG + chr4_2:48122417-48122436 None:intergenic 30.0%
! AGATATTTTCACCGAATGTA+CGG - chr4_2:48122573-48122592 Msa0603530:five_prime_UTR 30.0%
AAATGGCCAATTGGAGAAAA+CGG + chr4_2:48120316-48120335 None:intergenic 35.0%
!!! GAGTTTTTTTTTCAGAGAGG+GGG + chr4_2:48120342-48120361 None:intergenic 35.0%
!!! GGAGTTTTTTTTTCAGAGAG+GGG + chr4_2:48120343-48120362 None:intergenic 35.0%
!!! GTGGAGTTTTTTTTTCAGAG+AGG + chr4_2:48120345-48120364 None:intergenic 35.0%
! TGAGAGAGTGTGATTTGAAA+CGG + chr4_2:48120413-48120432 None:intergenic 35.0%
!!! CGATTTTGACTGTGTGAATT+TGG - chr4_2:48120558-48120577 Msa0603530:CDS 35.0%
TCTGATAGGTTGTTAGAGAT+CGG - chr4_2:48120605-48120624 Msa0603530:CDS 35.0%
GTAAATCCAACATTCTCTCT+AGG - chr4_2:48120712-48120731 Msa0603530:CDS 35.0%
TGTAAACCTAGAGAGAATGT+TGG + chr4_2:48120721-48120740 None:intergenic 35.0%
!! AGAAAATGGAAGTACCTGAA+GGG + chr4_2:48120802-48120821 None:intergenic 35.0%
!! GGTTCTGAATTCAGCATATT+TGG + chr4_2:48121155-48121174 None:intergenic 35.0%
AAGTTTCTGCTAGTACCTTT+CGG - chr4_2:48121232-48121251 Msa0603530:intron 35.0%
! TGAAGGCTTGTTTGAATTGA+TGG - chr4_2:48121297-48121316 Msa0603530:intron 35.0%
! ATTGATGGTATAAGGTGGAA+TGG - chr4_2:48121312-48121331 Msa0603530:intron 35.0%
CTACATTGATCTTAGCCAAA+AGG + chr4_2:48121411-48121430 None:intergenic 35.0%
TATGGTGTAATGAAGTGGAA+TGG + chr4_2:48121462-48121481 None:intergenic 35.0%
ACCATATATCACCATTCCAA+AGG - chr4_2:48121476-48121495 Msa0603530:intron 35.0%
!! ACCTTTGGAATGGTGATATA+TGG + chr4_2:48121480-48121499 None:intergenic 35.0%
AAACCTTTGACAATGTCAAC+CGG - chr4_2:48121862-48121881 Msa0603530:intron 35.0%
! TTTTCTCGAAACATCTGCAT+TGG - chr4_2:48122019-48122038 Msa0603530:CDS 35.0%
AAATCTCTGTCAATATGGTC+TGG + chr4_2:48122070-48122089 None:intergenic 35.0%
AGATGAATCTGCAACATTGA+TGG + chr4_2:48122165-48122184 None:intergenic 35.0%
AAGGAGCAAAAACAGAATTG+GGG + chr4_2:48122267-48122286 None:intergenic 35.0%
GAAAGGAGCAAAAACAGAAT+TGG + chr4_2:48122269-48122288 None:intergenic 35.0%
TGTTTATCTCTCACCCATAA+AGG + chr4_2:48122305-48122324 None:intergenic 35.0%
TAATGAGAATCTGACGTTTC+AGG - chr4_2:48122486-48122505 Msa0603530:five_prime_UTR 35.0%
AATGAGAATCTGACGTTTCA+GGG - chr4_2:48122487-48122506 Msa0603530:five_prime_UTR 35.0%
GAGTATAGGACAGAAGATGA+AGG + chr4_2:48120153-48120172 None:intergenic 40.0%
!! ACGGTGTCGTATTAGAGTAT+AGG + chr4_2:48120167-48120186 None:intergenic 40.0%
!! GAGTGAGAAAGCACTTACAA+CGG + chr4_2:48120283-48120302 None:intergenic 40.0%
!!! TTTTTTTTTCAGAGAGGGGG+TGG + chr4_2:48120339-48120358 None:intergenic 40.0%
AAAGAAGAAGAGGTAGAAGG+TGG + chr4_2:48120364-48120383 None:intergenic 40.0%
! AGTGTGATTTGAAACGGTTC+GGG + chr4_2:48120407-48120426 None:intergenic 40.0%
! GAGTGTGATTTGAAACGGTT+CGG + chr4_2:48120408-48120427 None:intergenic 40.0%
GAAGTTACCGTGAGAAGAAT+TGG + chr4_2:48120482-48120501 None:intergenic 40.0%
!! ATCGTTTTGATCGGAGATTC+TGG - chr4_2:48120684-48120703 Msa0603530:CDS 40.0%
!! GAGAAAATGGAAGTACCTGA+AGG + chr4_2:48120803-48120822 None:intergenic 40.0%
AATTCAGAACCCCAATGGTT+TGG - chr4_2:48121164-48121183 Msa0603530:intron 40.0%
TTCACTCAACCAAACCATTG+GGG + chr4_2:48121176-48121195 None:intergenic 40.0%
CTTCACTCAACCAAACCATT+GGG + chr4_2:48121177-48121196 None:intergenic 40.0%
ACTTCACTCAACCAAACCAT+TGG + chr4_2:48121178-48121197 None:intergenic 40.0%
GTACTAGCAGAAACTTGAAC+CGG + chr4_2:48121228-48121247 None:intergenic 40.0%
AGTTTCTGCTAGTACCTTTC+GGG - chr4_2:48121233-48121252 Msa0603530:intron 40.0%
TCAAACAAGCCTTCAAGCTT+CGG + chr4_2:48121292-48121311 None:intergenic 40.0%
AAGGTGGAATCGAATTCAGT+TGG + chr4_2:48121380-48121399 None:intergenic 40.0%
! GAATTCGATTCCACCTTTTC+CGG - chr4_2:48121383-48121402 Msa0603530:intron 40.0%
! AATTCGATTCCACCTTTTCC+GGG - chr4_2:48121384-48121403 Msa0603530:intron 40.0%
GAAGACAGAGAAACAACCAT+TGG + chr4_2:48121707-48121726 None:intergenic 40.0%
! AGACTGTAAAGGCACAGATT+TGG - chr4_2:48121745-48121764 Msa0603530:intron 40.0%
! GACTGTAAAGGCACAGATTT+GGG - chr4_2:48121746-48121765 Msa0603530:intron 40.0%
!! AATAGGCACTGGTAATTGCA+CGG + chr4_2:48121794-48121813 None:intergenic 40.0%
ATTACCAGTGCCTATTACAG+AGG - chr4_2:48121798-48121817 Msa0603530:intron 40.0%
TCACTATGTTAGAATCCGCA+TGG + chr4_2:48121908-48121927 None:intergenic 40.0%
AACATAGTGATCATGATGGC+AGG - chr4_2:48121918-48121937 Msa0603530:intron 40.0%
GAACCATCTTAGAGCTGTTT+CGG - chr4_2:48121956-48121975 Msa0603530:CDS 40.0%
TGAATCTGCAACATTGATGG+TGG + chr4_2:48122162-48122181 None:intergenic 40.0%
TCATCTGCCAATACAAAGAG+AGG - chr4_2:48122179-48122198 Msa0603530:intron 40.0%
AGGAGCAAAAACAGAATTGG+GGG + chr4_2:48122266-48122285 None:intergenic 40.0%
! TACTTTGTTGAGTTGCGATG+AGG - chr4_2:48122339-48122358 Msa0603530:five_prime_UTR 40.0%
! ACTAGGGTTGCTAGTTTCTT+TGG - chr4_2:48122382-48122401 Msa0603530:five_prime_UTR 40.0%
! TGGTGGGTGAATGAAGTACA+AGG + chr4_2:48120110-48120129 None:intergenic 45.0%
TAGGTTGTTAGAGATCGGAG+AGG - chr4_2:48120610-48120629 Msa0603530:CDS 45.0%
ATCGGAGAGGAGAAGATCTA+TGG - chr4_2:48120623-48120642 Msa0603530:CDS 45.0%
GAGAAGATCTATGGCGCATA+GGG - chr4_2:48120632-48120651 Msa0603530:CDS 45.0%
TTGATCGGAGATTCTGGTGT+TGG - chr4_2:48120690-48120709 Msa0603530:CDS 45.0%
TGCTGAATTCAGAACCCCAA+TGG - chr4_2:48121159-48121178 Msa0603530:intron 45.0%
AAACACTCACCGAAGCTTGA+AGG - chr4_2:48121280-48121299 Msa0603530:intron 45.0%
! ATTCGATTCCACCTTTTCCG+GGG - chr4_2:48121385-48121404 Msa0603530:intron 45.0%
!! ACCATTCCAAAGGTGTTCCT+TGG - chr4_2:48121486-48121505 Msa0603530:intron 45.0%
! TCCAAGGAACACCTTTGGAA+TGG + chr4_2:48121490-48121509 None:intergenic 45.0%
AGTGATGAGATGTCACTCCA+AGG + chr4_2:48121506-48121525 None:intergenic 45.0%
GGTAGAGGGAAAGACTGTAA+AGG - chr4_2:48121734-48121753 Msa0603530:intron 45.0%
ACCAACAGCTCCTCTGTAAT+AGG + chr4_2:48121811-48121830 None:intergenic 45.0%
GGAAGCAACCAACATTGAGA+AGG - chr4_2:48122040-48122059 Msa0603530:CDS 45.0%
CTGGAATGCCTTCTCAATGT+TGG + chr4_2:48122051-48122070 None:intergenic 45.0%
!! CAAAAAAGCACTTGCAGCTC+AGG - chr4_2:48122103-48122122 Msa0603530:intron 45.0%
!! CAACATTGATGGTGGTTCCT+TGG + chr4_2:48122154-48122173 None:intergenic 45.0%
ACAGCAGCCTCTCTTTGTAT+TGG + chr4_2:48122189-48122208 None:intergenic 45.0%
GGAGCAAAAACAGAATTGGG+GGG + chr4_2:48122265-48122284 None:intergenic 45.0%
AAAGGGGATGACCGTACATT+CGG + chr4_2:48122587-48122606 None:intergenic 45.0%
TGTGTATGCAGCTGGATGGT+GGG + chr4_2:48120126-48120145 None:intergenic 50.0%
TGACTGTGTATGCAGCTGGA+TGG + chr4_2:48120130-48120149 None:intergenic 50.0%
AAGGTGACTGTGTATGCAGC+TGG + chr4_2:48120134-48120153 None:intergenic 50.0%
TTTCAGAGAGGGGGTGGAAA+TGG + chr4_2:48120333-48120352 None:intergenic 50.0%
GGAGAAGATCTATGGCGCAT+AGG - chr4_2:48120631-48120650 Msa0603530:CDS 50.0%
AAGATCTATGGCGCATAGGG+TGG - chr4_2:48120635-48120654 Msa0603530:CDS 50.0%
! AAGTACCTGAAGGGTTCTGG+TGG + chr4_2:48120793-48120812 None:intergenic 50.0%
!! TGGAAGTACCTGAAGGGTTC+TGG + chr4_2:48120796-48120815 None:intergenic 50.0%
GTTTGTATGCACCATGTCGC+CGG - chr4_2:48121206-48121225 Msa0603530:intron 50.0%
AGAAACTTGAACCGGCGACA+TGG + chr4_2:48121220-48121239 None:intergenic 50.0%
TTACATTCACCTCGCCCGAA+AGG + chr4_2:48121250-48121269 None:intergenic 50.0%
TGATCTTAGCCAAAAGGCCC+CGG + chr4_2:48121405-48121424 None:intergenic 50.0%
GTCACTCCAAGGAACACCTT+TGG + chr4_2:48121495-48121514 None:intergenic 50.0%
GTTTCTCTGTCTTCCTCCAC+AGG - chr4_2:48121713-48121732 Msa0603530:intron 50.0%
TGTCTTCCTCCACAGGTAGA+GGG - chr4_2:48121720-48121739 Msa0603530:intron 50.0%
ACAGTCTTTCCCTCTACCTG+TGG + chr4_2:48121732-48121751 None:intergenic 50.0%
AGCTCCTCTGTAATAGGCAC+TGG + chr4_2:48121805-48121824 None:intergenic 50.0%
GCCTATTACAGAGGAGCTGT+TGG - chr4_2:48121807-48121826 Msa0603530:intron 50.0%
! CCACCGGTTGACATTGTCAA+AGG + chr4_2:48121868-48121887 None:intergenic 50.0%
CCTTTGACAATGTCAACCGG+TGG - chr4_2:48121865-48121884 Msa0603530:intron 50.0%
GCGTGAATTAAGGGACCATG+CGG - chr4_2:48121890-48121909 Msa0603530:intron 50.0%
!! CCTCCGAAACAGCTCTAAGA+TGG + chr4_2:48121962-48121981 None:intergenic 50.0%
! CCATCTTAGAGCTGTTTCGG+AGG - chr4_2:48121959-48121978 Msa0603530:CDS 50.0%
! AGAGCTGTTTCGGAGGATGA+CGG - chr4_2:48121966-48121985 Msa0603530:CDS 50.0%
!! CAAGCATTGGCAGAGAAGGA+AGG - chr4_2:48121990-48122009 Msa0603530:CDS 50.0%
GAGAATCTGACGTTTCAGGG+TGG - chr4_2:48122490-48122509 Msa0603530:five_prime_UTR 50.0%
GAATGTACGGTCATCCCCTT+TGG - chr4_2:48122586-48122605 Msa0603530:five_prime_UTR 50.0%
! TACGGTCATCCCCTTTGGAA+TGG - chr4_2:48122591-48122610 Msa0603530:five_prime_UTR 50.0%
CTGTGTATGCAGCTGGATGG+TGG + chr4_2:48120127-48120146 None:intergenic 55.0%
AGGGGGTGGAAATGGCCAAT+TGG + chr4_2:48120325-48120344 None:intergenic 55.0%
ATTCGCCACCAGAACCCTTC+AGG - chr4_2:48120785-48120804 Msa0603530:CDS 55.0%
TAGCCAAAAGGCCCCGGAAA+AGG + chr4_2:48121399-48121418 None:intergenic 55.0%
CTGTCTTCCTCCACAGGTAG+AGG - chr4_2:48121719-48121738 Msa0603530:intron 55.0%
GTCTTTCCCTCTACCTGTGG+AGG + chr4_2:48121729-48121748 None:intergenic 55.0%
! GCACAGATTTGGGACACAGC+CGG - chr4_2:48121756-48121775 Msa0603530:intron 55.0%
ACCGGTGGCTGCGTGAATTA+AGG - chr4_2:48121880-48121899 Msa0603530:intron 55.0%
CCCTTAATTCACGCAGCCAC+CGG + chr4_2:48121884-48121903 None:intergenic 55.0%
CCGGTGGCTGCGTGAATTAA+GGG - chr4_2:48121881-48121900 Msa0603530:intron 55.0%
! GGAGGATGACGGTCAAGCAT+TGG - chr4_2:48121977-48121996 Msa0603530:CDS 55.0%
!! CGGTCAAGCATTGGCAGAGA+AGG - chr4_2:48121986-48122005 Msa0603530:CDS 55.0%
!! TTGATGGTGGTTCCTTGGCC+AGG + chr4_2:48122149-48122168 None:intergenic 55.0%
CTGCTAGTACCTTTCGGGCG+AGG - chr4_2:48121238-48121257 Msa0603530:intron 60.0%
CCAAAAGGCCCCGGAAAAGG+TGG + chr4_2:48121396-48121415 None:intergenic 60.0%
! CCACCTTTTCCGGGGCCTTT+TGG - chr4_2:48121393-48121412 Msa0603530:intron 60.0%
! GATTTGGGACACAGCCGGTC+AGG - chr4_2:48121761-48121780 Msa0603530:intron 60.0%
CTTGCAGCTCAGGAAGCAGC+TGG - chr4_2:48122113-48122132 Msa0603530:intron 60.0%
GGTTCCTTGGCCAGGAAGTG+AGG + chr4_2:48122141-48122160 None:intergenic 60.0%
TGCACGGTACCGCTCCTGAC+CGG + chr4_2:48121778-48121797 None:intergenic 65.0%
GCAGCTGGTGCCTCACTTCC+TGG - chr4_2:48122128-48122147 Msa0603530:intron 65.0%
GGTGCCTCACTTCCTGGCCA+AGG - chr4_2:48122134-48122153 Msa0603530:intron 65.0%
GGGACACAGCCGGTCAGGAG+CGG - chr4_2:48121766-48121785 Msa0603530:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 48120094 48122635 48120094 ID=Msa0603530;Name=Msa0603530
chr4_2 mRNA 48120094 48122635 48120094 ID=Msa0603530-mRNA-1;Parent=Msa0603530;Name=Msa0603530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=429|1|1|1|0.5|0.33|3|418|215
chr4_2 exon 48120094 48120994 48120094 ID=Msa0603530-mRNA-1:exon:11085;Parent=Msa0603530-mRNA-1
chr4_2 exon 48121923 48122116 48121923 ID=Msa0603530-mRNA-1:exon:11084;Parent=Msa0603530-mRNA-1
chr4_2 exon 48122236 48122635 48122236 ID=Msa0603530-mRNA-1:exon:11083;Parent=Msa0603530-mRNA-1
chr4_2 five_prime_UTR 48122236 48122635 48122236 ID=Msa0603530-mRNA-1:five_prime_utr;Parent=Msa0603530-mRNA-1
chr4_2 five_prime_UTR 48122088 48122116 48122088 ID=Msa0603530-mRNA-1:five_prime_utr;Parent=Msa0603530-mRNA-1
chr4_2 CDS 48121923 48122087 48121923 ID=Msa0603530-mRNA-1:cds;Parent=Msa0603530-mRNA-1
chr4_2 CDS 48120512 48120994 48120512 ID=Msa0603530-mRNA-1:cds;Parent=Msa0603530-mRNA-1
chr4_2 three_prime_UTR 48120094 48120511 48120094 ID=Msa0603530-mRNA-1:three_prime_utr;Parent=Msa0603530-mRNA-1
Gene Sequence

>Msa0603530

ATGGCGCATAGGGTGGATCATGAGTATGATTACTTGTTCAAGATCGTTTTGATCGGAGATTCTGGTGTTGGTAAATCCAACATTCTCTCTAGGTTTACAAGAAATGAGTTTTGTTTGGAGTCTAAATCTACTATTGGTGTTGAATTCGCCACCAGAACCCTTCAGGTAGAGGGAAAGACTGTAAAGGCACAGATTTGGGACACAGCCGGTCAGGAGCGGTACCGTGCAATTACCAGTGCCTATTACAGAGGAGCTGTTGGTGCTCTTCTTGTATATGACATTACTAAGAGGCAAACCTTTGACAATGTCAACCGGTGGCTGCGTGAATTAAGGGACCATGCGGATTCTAACATAGTGATCATGATGGCAGGAAATAAGTCTGATTTGAACCATCTTAGAGCTGTTTCGGAGGATGACGGTCAAGCATTGGCAGAGAAGGAAGGTCTCTCTTTTCTCGAAACATCTGCATTGGAAGCAACCAACATTGAGAAGGCATTCCAGACCATATTGACAGAGATTTATCATATTGTAAGCAAAAAAGCACTTGCAGCTCAGGAAGCAGCTGGTGCCTCACTTCCTGGCCAAGGAACCACCATCAATGTTGCAGATTCATCTGCCAATACAAAGAGAGGCTGCTGTTCGACTTAA
Protein sequence

>Msa0603530

MAHRVDHEYDYLFKIVLIGDSGVGKSNILSRFTRNEFCLESKSTIGVEFATRTLQVEGKTVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKRQTFDNVNRWLRELRDHADSNIVIMMAGNKSDLNHLRAVSEDDGQALAEKEGLSFLETSALEATNIEKAFQTILTEIYHIVSKKALAAQEAAGASLPGQGTTINVADSSANTKRGCCST*