AlfalfaGEDB | Msa0644830

M. sativa cultivar zhongmu-4 / Msa0644830

KEGG: K07904|K07905|K07976
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005829 GO:0005886 GO:0005911 GO:0008150 GO:0009504 GO:0009506 GO:0009987 GO:0012505 GO:0016020 GO:0030054 GO:0031410 GO:0031982 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071554 GO:0071840 GO:0071944 GO:0097708
PFAM: PF00071.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0644830	XP_013456161.1	100.000	215	0	0	1	215	1	215	1.70e-157	444

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0644830	sp\|Q40193\|RB11C_LOTJA	95.833	216	8	1	1	215	1	216	4.21e-153	426

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0644830	I3S111	100.000	215	0	0	1	215	1	215	8.13e-158	444

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0137390	Msa0644830	0.818798	1.623992e-52	-8.615850e-47
Msa0182520	Msa0644830	0.846417	2.201099e-59	-8.615850e-47
Msa0347660	Msa0644830	0.800794	1.219236e-48	-8.615850e-47
Msa0442260	Msa0644830	0.805573	1.250874e-49	-8.615850e-47
Msa0467570	Msa0644830	0.823447	1.378547e-53	-8.615850e-47
Msa0514460	Msa0644830	0.806109	9.648629e-50	-8.615850e-47
Msa0603530	Msa0644830	0.847739	9.555896e-60	-8.615850e-47
Msa0644830	Msa0684340	0.914570	1.642045e-84	-8.615850e-47
Msa0644830	Msa1352590	0.823012	1.741285e-53	-8.615850e-47
Msa0644830	Msa1373070	0.827032	1.956528e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0644830	MtrunA17_Chr4g0032201	100.000	215	0	0	1	215	1	215	1.56e-161	444
Msa0644830	MtrunA17_Chr3g0112681	84.651	215	32	1	1	214	1	215	2.68e-134	375
Msa0644830	MtrunA17_Chr2g0276941	75.000	212	51	2	6	215	9	220	6.43e-117	332
Msa0644830	MtrunA17_Chr2g0315551	68.692	214	66	1	3	215	4	217	1.15e-109	313
Msa0644830	MtrunA17_Chr2g0283131	68.519	216	63	3	2	214	3	216	4.55e-106	304
Msa0644830	MtrunA17_Chr4g0046261	67.593	216	65	3	2	214	3	216	5.40e-105	301
Msa0644830	MtrunA17_Chr2g0319951	69.626	214	63	2	3	214	4	217	1.03e-104	301
Msa0644830	MtrunA17_Chr4g0040781	67.593	216	65	3	2	214	3	216	1.27e-104	300
Msa0644830	MtrunA17_Chr2g0325091	67.136	213	66	3	5	215	4	214	5.15e-101	291
Msa0644830	MtrunA17_Chr8g0381841	65.566	212	69	1	6	213	11	222	2.72e-97	282

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0644830	AT1G07410.1	90.233	215	20	1	1	215	1	214	1.83e-146	406
Msa0644830	AT3G46830.1	86.175	217	28	1	1	215	1	217	1.64e-136	381
Msa0644830	AT3G46830.2	86.175	217	28	1	1	215	1	217	1.64e-136	381
Msa0644830	AT5G59150.1	85.714	217	29	1	1	215	1	217	3.11e-136	380
Msa0644830	AT1G09630.1	74.537	216	54	1	1	215	1	216	5.66e-122	345
Msa0644830	AT4G18800.1	70.283	212	61	2	3	214	4	213	1.51e-109	313
Msa0644830	AT1G16920.1	69.626	214	63	2	2	214	3	215	8.75e-108	309
Msa0644830	AT5G45750.1	69.159	214	62	3	3	214	4	215	5.39e-107	306
Msa0644830	AT5G60860.1	68.981	216	62	3	2	214	3	216	7.16e-106	304
Msa0644830	AT1G06400.1	67.130	216	65	3	2	214	3	215	8.85e-104	298

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Find 96 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTTTGACAATGTCAAC+CGG	0.533316	4_3:-49200188	Msa0644830:CDS
AAAGAAGAAGAAGAGGTAGA+AGG	0.435766	4_3:+49201681	None:intergenic
AAATCTCTGTCAATATGGTC+TGG	0.463166	4_3:+49199983	None:intergenic
AAATGGCCAATTGGAGAAAA+CGG	0.492482	4_3:+49201731	None:intergenic
AACATAGTGATCATGATGGC+AGG	0.549455	4_3:-49200132	Msa0644830:CDS
AAGATCTATGGCGCATAGGG+TGG	0.554795	4_3:-49201407	Msa0644830:exon
AAGTACCTGAAGGGTTCTGG+TGG	0.684017	4_3:+49201252	None:intergenic
AATAGGCACTGGTAATTGCA+CGG	0.632997	4_3:+49200259	None:intergenic
AATCAATCCAATTCTTCTCA+CGG	0.593636	4_3:-49201570	Msa0644830:intron
AATGAGAATCTGACGTTTCA+GGG	0.426836	4_3:-49199563	Msa0644830:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGAAGAATTGGATTGATT+TGG	+	chr4_3:49199825-49199844	None:intergenic	30.0%
AATCAATCCAATTCTTCTCA+CGG	-	chr4_3:49199825-49199844	Msa0644830:three_prime_UTR	30.0%
ATCTAATAACTGAATCGGAA+AGG	+	chr4_3:49199862-49199881	None:intergenic	30.0%
TTGGAGTCTAAATCTACTAT+TGG	-	chr4_3:49200109-49200128	Msa0644830:CDS	30.0%
AGAAATCAACAGAGAGAAAA+TGG	+	chr4_3:49200169-49200188	None:intergenic	30.0%
CCAATAAAATTACATTCACC+TGG	+	chr4_3:49200613-49200632	None:intergenic	30.0%
TTGTTTGAACTGATGGTATA+AGG	-	chr4_3:49200658-49200677	Msa0644830:intron	30.0%
AAGGTGGAATCAAATTTAGT+TGG	+	chr4_3:49200734-49200753	None:intergenic	30.0%
TGATATATGGTGTAATGAAG+TGG	+	chr4_3:49200821-49200840	None:intergenic	30.0%
GGTACATTGTAATACAAACA+AGG	+	chr4_3:49201031-49201050	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	49199440	49201977	49199440	ID=Msa0644830;Name=Msa0644830
chr4_3	mRNA	49199440	49201977	49199440	ID=Msa0644830-mRNA-1;Parent=Msa0644830;Name=Msa0644830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=436\|1\|1\|1\|0.5\|0.33\|3\|415\|215
chr4_3	exon	49199440	49200337	49199440	ID=Msa0644830-mRNA-1:exon:11738;Parent=Msa0644830-mRNA-1
chr4_3	exon	49201258	49201451	49201258	ID=Msa0644830-mRNA-1:exon:11737;Parent=Msa0644830-mRNA-1
chr4_3	exon	49201571	49201977	49201571	ID=Msa0644830-mRNA-1:exon:11736;Parent=Msa0644830-mRNA-1
chr4_3	five_prime_UTR	49201571	49201977	49201571	ID=Msa0644830-mRNA-1:five_prime_utr;Parent=Msa0644830-mRNA-1
chr4_3	five_prime_UTR	49201423	49201451	49201423	ID=Msa0644830-mRNA-1:five_prime_utr;Parent=Msa0644830-mRNA-1
chr4_3	CDS	49201258	49201422	49201258	ID=Msa0644830-mRNA-1:cds;Parent=Msa0644830-mRNA-1
chr4_3	CDS	49199855	49200337	49199855	ID=Msa0644830-mRNA-1:cds;Parent=Msa0644830-mRNA-1
chr4_3	three_prime_UTR	49199440	49199854	49199440	ID=Msa0644830-mRNA-1:three_prime_utr;Parent=Msa0644830-mRNA-1

Gene Sequence

>Msa0644830

ATGGCGCATAGGGTGGATCATGAGTATGATTACTTGTTCAAGATCGTTTTGATCGGAGATTCTGGTGTTGGTAAATCCAACATTCTCTCTAGGTTTACAAGAAATGAGTTTTGTTTGGAGTCTAAATCTACTATTGGTGTTGAATTCGCCACCAGAACCCTTCAGGTAGAGGGAAAGACTGTAAAGGCACAGATTTGGGACACAGCCGGTCAGGAGCGGTACCGTGCAATTACCAGTGCCTATTACAGAGGAGCTGTTGGTGCTCTTCTTGTATATGACATTACTAAGAGGCAAACCTTTGACAATGTCAACCGGTGGCTGCGTGAATTAAGGGACCATGCGGATTCTAACATAGTGATCATGATGGCAGGAAATAAGTCTGATTTGAACCATCTTAGAGCTGTTTCGGAGGATGACGGTCAAGCATTGGCAGAGAAGGAAGGTCTCTCTTTTCTCGAGACATCTGCATTGGAAGCAACCAACATTGAGAAGGCATTCCAGACCATATTGACAGAGATTTATCATATTGTAAGCAAAAAAGCACTTGCAGCTCAGGAAGCAGCTGGTACCTCACTTCCTGGCCAAGGAACCACCATCAATGTTGCAGATTCATCTGCCAATACAAAGAGAGGCTGCTGTTCGACTTAA

Protein sequence

>Msa0644830

MAHRVDHEYDYLFKIVLIGDSGVGKSNILSRFTRNEFCLESKSTIGVEFATRTLQVEGKTVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKRQTFDNVNRWLRELRDHADSNIVIMMAGNKSDLNHLRAVSEDDGQALAEKEGLSFLETSALEATNIEKAFQTILTEIYHIVSKKALAAQEAAGTSLPGQGTTINVADSSANTKRGCCST*