AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605310 MtrunA17_Chr4g0034511 97.849 372 8 0 1 372 1 372 0.0 752
Msa0605310 MtrunA17_Chr1g0154581 61.853 367 139 1 1 366 1 367 5.86e-170 479
Msa0605310 MtrunA17_Chr1g0173711 38.095 231 140 3 98 326 84 313 1.96e-48 168
Msa0605310 MtrunA17_Chr7g0258001 33.065 248 163 2 96 340 82 329 9.06e-46 161
Msa0605310 MtrunA17_Chr2g0328811 37.864 206 125 2 96 299 82 286 7.34e-45 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605310 AT3G18850.1 63.202 356 129 1 18 371 12 367 5.59e-176 494
Msa0605310 AT3G18850.4 63.202 356 129 1 18 371 12 367 5.59e-176 494
Msa0605310 AT3G18850.2 63.202 356 129 1 18 371 12 367 5.59e-176 494
Msa0605310 AT3G18850.3 63.202 356 129 1 18 371 12 367 5.59e-176 494
Msa0605310 AT3G18850.5 63.303 327 118 1 47 371 18 344 1.31e-161 457
Msa0605310 AT1G75020.2 60.446 359 139 2 14 371 13 369 1.67e-157 447
Msa0605310 AT1G75020.1 60.446 359 139 2 14 371 13 369 1.67e-157 447
Msa0605310 AT3G18850.6 56.818 220 93 1 154 371 17 236 9.39e-94 280
Msa0605310 AT3G57650.1 36.820 239 147 2 97 331 82 320 4.94e-49 170
Msa0605310 AT1G51260.1 30.696 316 207 7 28 337 12 321 6.21e-41 148

Find 100 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTCGACTGGATGTATTTA+TGG 0.192495 4_2:-51144129 Msa0605310:CDS
TCTTTCTTGGACAATGTATT+TGG 0.227806 4_2:-51143030 Msa0605310:CDS
AGAAAGTGAGGTGTAGATTT+TGG 0.233514 4_2:+51146103 None:intergenic
GATGGATAGATTCCGATTTA+AGG 0.239062 4_2:-51142920 Msa0605310:CDS
GTCTGTAGTCGTCGCTTATT+TGG 0.253949 4_2:-51142734 Msa0605310:CDS
ATACCGCTGCCCGTCTTTCT+TGG 0.257600 4_2:-51143043 Msa0605310:CDS
TACGTAAAATCAGTGCCTTC+AGG 0.315013 4_2:+51143902 None:intergenic
AAGGGGTTTGGTGTGTCTAT+TGG 0.322223 4_2:-51144383 Msa0605310:CDS
ATTCGGCCACAACCCATTCT+TGG 0.333164 4_2:-51142691 Msa0605310:CDS
CTTGTTTCTGATAGCATATA+TGG 0.341553 4_2:-51144473 Msa0605310:exon
TGAAATTTCTACGTGGTTGA+TGG 0.356721 4_2:-51142938 Msa0605310:CDS
AAACCCCTTAGAAATCTAAA+AGG 0.358797 4_2:+51144397 None:intergenic
GCTTCCCACTTCCTTTCCAC+TGG 0.362237 4_2:+51143998 None:intergenic
TTTGGTGTGTCTATTGGTGC+TGG 0.382243 4_2:-51144377 Msa0605310:CDS
GGATGGGAAAGCGGCAGATA+TGG 0.384803 4_2:+51142983 None:intergenic
CGGCTACATATTTCAACATT+CGG 0.392577 4_2:-51142708 Msa0605310:CDS
ACAAGAGTTTGATGAGAGAT+TGG 0.394543 4_2:+51146007 None:intergenic
AGTCGTCGCTTATTTGGTTC+CGG 0.394861 4_2:-51142728 Msa0605310:CDS
AGCAAGCCAGAGAGGATCTT+GGG 0.400056 4_2:+51143931 None:intergenic
TTTAAAATGCCCTTCACAGC+AGG 0.401277 4_2:+51142832 None:intergenic
TTGGTACTTGGCTAACTTTG+TGG 0.403348 4_2:-51144252 Msa0605310:CDS
TTTGTAACATTGGTGTTCTT+CGG 0.407274 4_2:-51144343 Msa0605310:CDS
TTGATCTCAATGTTTGTTTG+TGG 0.410312 4_2:-51145982 Msa0605310:five_prime_UTR
ATGAAACATTCTCCAACATC+AGG 0.411393 4_2:+51142539 None:intergenic
AAAGCAACTTGTCCTTAAAT+CGG 0.413361 4_2:+51142908 None:intergenic
GAAATTTCATCTTCAGATGT+TGG 0.414236 4_2:+51142952 None:intergenic
AAATTTCATCTTCAGATGTT+GGG 0.418091 4_2:+51142953 None:intergenic
CTTCCCACTTCCTTTCCACT+GGG 0.434035 4_2:+51143999 None:intergenic
AAGACTAAAGTTGTGTTCTC+TGG 0.436607 4_2:-51144205 Msa0605310:CDS
GCCTCGCAACTCTTGCAAAC+AGG 0.445731 4_2:+51143414 None:intergenic
ACGCGTGTAACTTCAAAACT+TGG 0.456940 4_2:-51144442 Msa0605310:CDS
AGTCGACTGGATGTATTTAT+GGG 0.457387 4_2:-51144128 Msa0605310:CDS
TTCATCAGCATTTGTAACAT+TGG 0.466713 4_2:-51144353 Msa0605310:CDS
TAGCAAGCCAGAGAGGATCT+TGG 0.476050 4_2:+51143930 None:intergenic
ACAAGCCTGGATTGACAGAA+TGG 0.486210 4_2:+51144520 None:intergenic
ATGATCCCCAAGATCCTCTC+TGG 0.492077 4_2:-51143937 Msa0605310:CDS
AAGATAGAATTAAGAAAGTG+AGG 0.497353 4_2:+51146091 None:intergenic
ACGTAAAATCAGTGCCTTCA+GGG 0.505273 4_2:+51143903 None:intergenic
ATATTCCATTCTGTCAATCC+AGG 0.506999 4_2:-51144525 Msa0605310:five_prime_UTR
TCAGTTTACGATGTTACCAT+TGG 0.507137 4_2:-51143072 Msa0605310:intron
GAGGGACCTCCCTGCTGTGA+AGG 0.507648 4_2:-51142842 Msa0605310:CDS
CAAACAGGTACAAAAGCCCT+TGG 0.513559 4_2:+51143429 None:intergenic
TCATCCCAGTGGAAAGGAAG+TGG 0.514537 4_2:-51144003 Msa0605310:CDS
GCACCTAGGGGAAAGTAGAC+TGG 0.517923 4_2:+51144635 None:intergenic
ATACAATATATTAACAAGCC+TGG 0.520410 4_2:+51144507 None:intergenic
CTTGGACAATGTATTTGGAG+TGG 0.522752 4_2:-51143025 Msa0605310:CDS
ATGGGACCTTGCGTTGCGGA+AGG 0.532796 4_2:-51144110 Msa0605310:CDS
AGATGTTGGGATACAGTGGA+TGG 0.536671 4_2:+51142966 None:intergenic
CATCCAGTCTACTTTCCCCT+AGG 0.538508 4_2:-51144638 Msa0605310:five_prime_UTR
TGTTTCCTTAGCCCTGATGT+TGG 0.542747 4_2:-51142551 Msa0605310:three_prime_UTR
GATACGATAGATCGCACCTA+GGG 0.544055 4_2:+51144622 None:intergenic
ACATCCAGTCGACTTCAGTT+CGG 0.547137 4_2:+51144137 None:intergenic
AGGGCTAAGGAAACAATCTT+CGG 0.547167 4_2:+51142559 None:intergenic
GTGCAACTTTGAAACAGTTG+TGG 0.550821 4_2:-51146186 Msa0605310:five_prime_UTR
AGGGACCTCCCTGCTGTGAA+GGG 0.552077 4_2:-51142841 Msa0605310:CDS
GTGACAGTGATTATATTGAC+TGG 0.553389 4_2:-51142805 Msa0605310:CDS
ATATTGTTCCTTCGAGAGAA+CGG 0.553559 4_2:-51144180 Msa0605310:CDS
TGTCCAAGAAAGACGGGCAG+CGG 0.556324 4_2:+51143040 None:intergenic
ATCACCGAACTGAAGTCGAC+TGG 0.565240 4_2:-51144141 Msa0605310:CDS
TGGGACCTTGCGTTGCGGAA+GGG 0.565717 4_2:-51144109 Msa0605310:CDS
ATACATTGTCCAAGAAAGAC+GGG 0.565950 4_2:+51143034 None:intergenic
TTGCGAGGCTCTCTAAATGC+AGG 0.568276 4_2:-51143400 Msa0605310:intron
TGCATCCCTTCCGCAACGCA+AGG 0.570372 4_2:+51144104 None:intergenic
ATTCTCCAACATCAGGGCTA+AGG 0.574486 4_2:+51142546 None:intergenic
AGCACAATCCGTTCTCTCGA+AGG 0.580059 4_2:+51144172 None:intergenic
AATACATTGTCCAAGAAAGA+CGG 0.589227 4_2:+51143033 None:intergenic
CGAATGTTGAAATATGTAGC+CGG 0.590553 4_2:+51142709 None:intergenic
CAACTTTGAAACAGTTGTGG+AGG 0.591892 4_2:-51146183 Msa0605310:five_prime_UTR
ATGTACAAGGACAAAGCCAC+TGG 0.592436 4_2:-51144599 Msa0605310:five_prime_UTR
AATGTTACAAGCTTGATCAA+AGG 0.596369 4_2:-51145952 Msa0605310:intron
TCTGCATATCACTCTGCCAG+TGG 0.604292 4_2:+51144583 None:intergenic
CTTCAGATGTTGGGATACAG+TGG 0.606141 4_2:+51142962 None:intergenic
ACCTGTTTGCAAGAGTTGCG+AGG 0.606957 4_2:-51143415 Msa0605310:CDS
TTAAAATGCCCTTCACAGCA+GGG 0.607039 4_2:+51142833 None:intergenic
TAGAGTTCATCCCAGTGGAA+AGG 0.609644 4_2:-51144009 Msa0605310:CDS
GCAGATATGGATATGAACTT+CGG 0.613202 4_2:+51142996 None:intergenic
CATCCCAGTGGAAAGGAAGT+GGG 0.613686 4_2:-51144002 Msa0605310:CDS
GGATACAGTGGATGGGAAAG+CGG 0.620018 4_2:+51142974 None:intergenic
ATATGGATATGAACTTCGGA+AGG 0.623808 4_2:+51143000 None:intergenic
GATCTATCGTATCATGTACA+AGG 0.624221 4_2:-51144612 Msa0605310:five_prime_UTR
GCGTTGCGGAAGGGATGCAT+TGG 0.626115 4_2:-51144100 Msa0605310:CDS
TCCTGATCAAGCAAAAGAGA+GGG 0.626456 4_2:-51142860 Msa0605310:CDS
GGGAAAATAGCAAGCCAGAG+AGG 0.628084 4_2:+51143923 None:intergenic
TTCCTGATCAAGCAAAAGAG+AGG 0.628899 4_2:-51142861 Msa0605310:CDS
GCAGCGGTATTTGTAGCCAA+TGG 0.634421 4_2:+51143056 None:intergenic
AACTTTGAAACAGTTGTGGA+GGG 0.636078 4_2:-51146182 Msa0605310:five_prime_UTR
GTTTCTGATAGCATATATGG+CGG 0.637213 4_2:-51144470 Msa0605310:exon
AAAACTCCAAGAATGGGTTG+TGG 0.641853 4_2:+51142685 None:intergenic
GATGTTGGGATACAGTGGAT+GGG 0.645947 4_2:+51142967 None:intergenic
TGATACGATAGATCGCACCT+AGG 0.651079 4_2:+51144621 None:intergenic
TGAAACATTCTCCAACATCA+GGG 0.655228 4_2:+51142540 None:intergenic
GCAAGCCAGAGAGGATCTTG+GGG 0.667193 4_2:+51143932 None:intergenic
ATTTATGGGACCTTGCGTTG+CGG 0.679165 4_2:-51144114 Msa0605310:CDS
GATATGCAGAACAAACTCCA+AGG 0.686790 4_2:-51144570 Msa0605310:five_prime_UTR
GTAAAGTTTAAACCAGACAG+AGG 0.706270 4_2:+51142759 None:intergenic
CATACTAGAGTTCATCCCAG+TGG 0.720097 4_2:-51144014 Msa0605310:CDS
AAATGCCCTTCACAGCAGGG+AGG 0.725667 4_2:+51142836 None:intergenic
ATACGATAGATCGCACCTAG+GGG 0.736068 4_2:+51144623 None:intergenic
CTGAAGATGAAATTTCTACG+TGG 0.745759 4_2:-51142945 Msa0605310:CDS
ATTCACTATTCATCAAAACG+AGG 0.752063 4_2:-51146059 Msa0605310:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTTATTTTTTCTTCT+GGG + chr4_2:51143734-51143753 None:intergenic 15.0%
!!! ATCATTTTTATTTTTTCTTC+TGG + chr4_2:51143735-51143754 None:intergenic 15.0%
!!! TTGATTTTTTCAAATAGAAA+GGG + chr4_2:51144491-51144510 None:intergenic 15.0%
!! AATGAAAATCTATTAAAAAG+TGG + chr4_2:51144843-51144862 None:intergenic 15.0%
!!! TTCCATTTTTTTTCCTTATA+TGG - chr4_2:51142964-51142983 Msa0605310:CDS 20.0%
!! TACCATATAAGGAAAAAAAA+TGG + chr4_2:51142969-51142988 None:intergenic 20.0%
!! CTATCATTGCTTATAATAAA+GGG + chr4_2:51143890-51143909 None:intergenic 20.0%
!! GAATATCTTCTATGAAAAAT+TGG + chr4_2:51144178-51144197 None:intergenic 20.0%
!!! GTTGATTTTTTCAAATAGAA+AGG + chr4_2:51144492-51144511 None:intergenic 20.0%
!! CTTAAAAAATTGTCTGAATT+TGG + chr4_2:51144893-51144912 None:intergenic 20.0%
!!! AGTAAAACATTTTTCAGAAT+CGG + chr4_2:51145260-51145279 None:intergenic 20.0%
!!! CTTTACAATTTTCAATTTCA+AGG - chr4_2:51145828-51145847 Msa0605310:intron 20.0%
! AAGATAGAATTAAGAAAGTG+AGG + chr4_2:51142629-51142648 None:intergenic 25.0%
!! TTTTCCCTTTTTTTCTTTCT+GGG + chr4_2:51142995-51143014 None:intergenic 25.0%
!! TTTTTCCCTTTTTTTCTTTC+TGG + chr4_2:51142996-51143015 None:intergenic 25.0%
! TCAGATTTCTATGTATTGTT+AGG - chr4_2:51143098-51143117 Msa0605310:intron 25.0%
!! ATTCAATCCTTTTACTTTCT+CGG - chr4_2:51143329-51143348 Msa0605310:intron 25.0%
! TAATAATCCGAGAAAGTAAA+AGG + chr4_2:51143339-51143358 None:intergenic 25.0%
! TTACTTTCTCGGATTATTAT+TGG - chr4_2:51143340-51143359 Msa0605310:intron 25.0%
! TACTTTGTATGTAATTCAGT+TGG - chr4_2:51143395-51143414 Msa0605310:intron 25.0%
! AAGATAATTCTACCATTTAC+TGG + chr4_2:51143563-51143582 None:intergenic 25.0%
! TGTTACCTCAAAATTCAAAA+GGG + chr4_2:51143628-51143647 None:intergenic 25.0%
! TTGTTACCTCAAAATTCAAA+AGG + chr4_2:51143629-51143648 None:intergenic 25.0%
!! TTTTGCGATATTATGTATCA+TGG + chr4_2:51143794-51143813 None:intergenic 25.0%
! ACATAATATCGCAAAAATGT+TGG - chr4_2:51143798-51143817 Msa0605310:intron 25.0%
! GCTATCATTGCTTATAATAA+AGG + chr4_2:51143891-51143910 None:intergenic 25.0%
! ATACAATATATTAACAAGCC+TGG + chr4_2:51144213-51144232 None:intergenic 25.0%
! CACAATTCAAATAGTTAATG+AGG - chr4_2:51144920-51144939 Msa0605310:intron 25.0%
! TACAAGAAGAAAATGATAGA+TGG + chr4_2:51145187-51145206 None:intergenic 25.0%
! TTCCATGTATATGCAATTAT+TGG - chr4_2:51145361-51145380 Msa0605310:intron 25.0%
! AACCAATAATTGCATATACA+TGG + chr4_2:51145366-51145385 None:intergenic 25.0%
! AATGATATCAGATTGATAGA+AGG - chr4_2:51145556-51145575 Msa0605310:intron 25.0%
! AGATTGATAGAAGGTTAAAA+AGG - chr4_2:51145565-51145584 Msa0605310:intron 25.0%
! AAATTTCATCTTCAGATGTT+GGG + chr4_2:51145767-51145786 None:intergenic 25.0%
! ATCTTAAAAACTCCAAGAAT+GGG + chr4_2:51146041-51146060 None:intergenic 25.0%
ATTCACTATTCATCAAAACG+AGG - chr4_2:51142658-51142677 Msa0605310:exon 30.0%
! GATTGGAAAATGAGCTTTTA+AGG + chr4_2:51142696-51142715 None:intergenic 30.0%
TTGATCTCAATGTTTGTTTG+TGG - chr4_2:51142735-51142754 Msa0605310:CDS 30.0%
AATGTTACAAGCTTGATCAA+AGG - chr4_2:51142765-51142784 Msa0605310:CDS 30.0%
TTGCTTGATGAATGCTTTAT+TGG - chr4_2:51142829-51142848 Msa0605310:CDS 30.0%
! TGCTTGATGAATGCTTTATT+GGG - chr4_2:51142830-51142849 Msa0605310:CDS 30.0%
!!! TCAAACCTTTTTTTTGTCTG+TGG - chr4_2:51142880-51142899 Msa0605310:CDS 30.0%
CTTTACCACAGACAAAAAAA+AGG + chr4_2:51142888-51142907 None:intergenic 30.0%
! GGAAGTAACTATGATGTTTT+TGG - chr4_2:51142926-51142945 Msa0605310:CDS 30.0%
!!! AACTATGATGTTTTTGGAAC+TGG - chr4_2:51142932-51142951 Msa0605310:CDS 30.0%
!!! ACTATGATGTTTTTGGAACT+GGG - chr4_2:51142933-51142952 Msa0605310:CDS 30.0%
TAAACCCCAGAAAGAAAAAA+AGG - chr4_2:51142987-51143006 Msa0605310:CDS 30.0%
AAACCCCAGAAAGAAAAAAA+GGG - chr4_2:51142988-51143007 Msa0605310:CDS 30.0%
!!! TTTCCCTTTTTTTCTTTCTG+GGG + chr4_2:51142994-51143013 None:intergenic 30.0%
! ACCTTTTCATTTGCATAATG+TGG - chr4_2:51143022-51143041 Msa0605310:CDS 30.0%
ACCACATTATGCAAATGAAA+AGG + chr4_2:51143026-51143045 None:intergenic 30.0%
! TAGACTTATGTGATGTCTTT+TGG - chr4_2:51143154-51143173 Msa0605310:intron 30.0%
! TTATGTGATGTCTTTTGGTA+TGG - chr4_2:51143159-51143178 Msa0605310:intron 30.0%
TGGATCACTTGAAAAATGAT+AGG - chr4_2:51143179-51143198 Msa0605310:intron 30.0%
GGATCACTTGAAAAATGATA+GGG - chr4_2:51143180-51143199 Msa0605310:intron 30.0%
!! GGATTATTATTGGTGATGTT+TGG - chr4_2:51143350-51143369 Msa0605310:intron 30.0%
TTCTAGTCTATCATGTCTTT+AGG - chr4_2:51143432-51143451 Msa0605310:CDS 30.0%
TAAAAGTTCACCTCACTTAA+TGG - chr4_2:51143458-51143477 Msa0605310:CDS 30.0%
! TATTGTTGTTGCATGTCTTT+AGG - chr4_2:51143501-51143520 Msa0605310:CDS 30.0%
GTTCCTAATAATCCAGTAAA+TGG - chr4_2:51143548-51143567 Msa0605310:intron 30.0%
CTACCATTTACTGGATTATT+AGG + chr4_2:51143554-51143573 None:intergenic 30.0%
! GAAATCCCTTTTGAATTTTG+AGG - chr4_2:51143620-51143639 Msa0605310:intron 30.0%
TGTCAAAGTTGTAGTGAAAT+TGG + chr4_2:51143654-51143673 None:intergenic 30.0%
ACCATTCAATTGTTTGACAT+TGG + chr4_2:51143706-51143725 None:intergenic 30.0%
CATACATAATAGGAGAAGAA+GGG + chr4_2:51143962-51143981 None:intergenic 30.0%
TTCTCCTATTATGTATGCTT+AGG - chr4_2:51143965-51143984 Msa0605310:CDS 30.0%
AAAGCCTAAGCATACATAAT+AGG + chr4_2:51143972-51143991 None:intergenic 30.0%
AAATGTAATGAAGGACTGAA+TGG + chr4_2:51144047-51144066 None:intergenic 30.0%
! AGTCCTTCATTACATTTTTC+AGG - chr4_2:51144050-51144069 Msa0605310:CDS 30.0%
! GTCCTTCATTACATTTTTCA+GGG - chr4_2:51144051-51144070 Msa0605310:CDS 30.0%
!! CTTGTTTCTGATAGCATATA+TGG - chr4_2:51144244-51144263 Msa0605310:CDS 30.0%
! TAGCTCCTTTTAGATTTCTA+AGG - chr4_2:51144315-51144334 Msa0605310:CDS 30.0%
! AGCTCCTTTTAGATTTCTAA+GGG - chr4_2:51144316-51144335 Msa0605310:CDS 30.0%
AAACCCCTTAGAAATCTAAA+AGG + chr4_2:51144323-51144342 None:intergenic 30.0%
!! TTTTAGATTTCTAAGGGGTT+TGG - chr4_2:51144322-51144341 Msa0605310:CDS 30.0%
TTCATCAGCATTTGTAACAT+TGG - chr4_2:51144364-51144383 Msa0605310:CDS 30.0%
! TTTGTAACATTGGTGTTCTT+CGG - chr4_2:51144374-51144393 Msa0605310:CDS 30.0%
!!! AAAACAACGTCGTTTTTCTT+TGG - chr4_2:51144446-51144465 Msa0605310:CDS 30.0%
! TTGATGAGACTTCCAATTTT+TGG - chr4_2:51144668-51144687 Msa0605310:intron 30.0%
! GGAATCATATGATTTTGTCA+CGG - chr4_2:51145017-51145036 Msa0605310:intron 30.0%
ATAATCCTTGGTTGAGTATA+AGG + chr4_2:51145422-51145441 None:intergenic 30.0%
AATACATTGTCCAAGAAAGA+CGG + chr4_2:51145687-51145706 None:intergenic 30.0%
TCTTTCTTGGACAATGTATT+TGG - chr4_2:51145687-51145706 Msa0605310:intron 30.0%
GAAATTTCATCTTCAGATGT+TGG + chr4_2:51145768-51145787 None:intergenic 30.0%
AAAGCAACTTGTCCTTAAAT+CGG + chr4_2:51145812-51145831 None:intergenic 30.0%
CATCTTAAAAACTCCAAGAA+TGG + chr4_2:51146042-51146061 None:intergenic 30.0%
!! AACTTTGAAACAGTTGTGGA+GGG - chr4_2:51142535-51142554 Msa0605310:three_prime_UTR 35.0%
! AGAAAGTGAGGTGTAGATTT+TGG + chr4_2:51142617-51142636 None:intergenic 35.0%
ACAAGAGTTTGATGAGAGAT+TGG + chr4_2:51142713-51142732 None:intergenic 35.0%
! GCTTGATGAATGCTTTATTG+GGG - chr4_2:51142831-51142850 Msa0605310:CDS 35.0%
TCATAGTTACTTCCAAAGCT+CGG + chr4_2:51142920-51142939 None:intergenic 35.0%
!!! CTATGATGTTTTTGGAACTG+GGG - chr4_2:51142934-51142953 Msa0605310:CDS 35.0%
!! TTTCTGGGGTTTACCATATA+AGG + chr4_2:51142980-51142999 None:intergenic 35.0%
! TTTTTCTGTGTGATCACACA+TGG - chr4_2:51143073-51143092 Msa0605310:intron 35.0%
TTCAGATCGAAGACATATCT+AGG - chr4_2:51143270-51143289 Msa0605310:intron 35.0%
!! ATCTAGGTTTTGACATGTGT+CGG - chr4_2:51143286-51143305 Msa0605310:intron 35.0%
AGACATGATAGACTAGAATG+TGG + chr4_2:51143430-51143449 None:intergenic 35.0%
TTCACCTCACTTAATGGAAA+AGG - chr4_2:51143464-51143483 Msa0605310:CDS 35.0%
! AAGTCCTTTTCCATTAAGTG+AGG + chr4_2:51143471-51143490 None:intergenic 35.0%
CACTTAATGGAAAAGGACTT+TGG - chr4_2:51143471-51143490 Msa0605310:CDS 35.0%
TTAATGGAAAAGGACTTTGG+TGG - chr4_2:51143474-51143493 Msa0605310:CDS 35.0%
! TGCTGTTTGATTCTTACTAC+TGG - chr4_2:51143598-51143617 Msa0605310:intron 35.0%
GCCAATGTCAAACAATTGAA+TGG - chr4_2:51143702-51143721 Msa0605310:intron 35.0%
GCGATATTATGTATCATGGA+AGG + chr4_2:51143790-51143809 None:intergenic 35.0%
CAACATCATTGAACTCCAAA+AGG + chr4_2:51143865-51143884 None:intergenic 35.0%
GCATACATAATAGGAGAAGA+AGG + chr4_2:51143963-51143982 None:intergenic 35.0%
CACCCTGAAAAATGTAATGA+AGG + chr4_2:51144056-51144075 None:intergenic 35.0%
GATCTATCGTATCATGTACA+AGG - chr4_2:51144105-51144124 Msa0605310:CDS 35.0%
! ATGAAAAATTGGTCTAGCCT+TGG + chr4_2:51144167-51144186 None:intergenic 35.0%
ATATTCCATTCTGTCAATCC+AGG - chr4_2:51144192-51144211 Msa0605310:CDS 35.0%
GTTTCTGATAGCATATATGG+CGG - chr4_2:51144247-51144266 Msa0605310:CDS 35.0%
! GCTCCTTTTAGATTTCTAAG+GGG - chr4_2:51144317-51144336 Msa0605310:CDS 35.0%
!! TTTTTAGCATCAGTTACAGC+AGG - chr4_2:51144423-51144442 Msa0605310:CDS 35.0%
AAGACTAAAGTTGTGTTCTC+TGG - chr4_2:51144512-51144531 Msa0605310:five_prime_UTR 35.0%
ATATTGTTCCTTCGAGAGAA+CGG - chr4_2:51144537-51144556 Msa0605310:five_prime_UTR 35.0%
AAGTCGACTGGATGTATTTA+TGG - chr4_2:51144588-51144607 Msa0605310:five_prime_UTR 35.0%
AGTCGACTGGATGTATTTAT+GGG - chr4_2:51144589-51144608 Msa0605310:five_prime_UTR 35.0%
!!! TGAGACTTCCAATTTTTGGT+TGG - chr4_2:51144672-51144691 Msa0605310:intron 35.0%
!!! GAGACTTCCAATTTTTGGTT+GGG - chr4_2:51144673-51144692 Msa0605310:intron 35.0%
TGAAATGCCCAACCAAAAAT+TGG + chr4_2:51144683-51144702 None:intergenic 35.0%
GTAGTCAGGTTTCTTGTATT+TGG - chr4_2:51144996-51145015 Msa0605310:intron 35.0%
AAAGTCACCATTCGAAGTAT+GGG + chr4_2:51145077-51145096 None:intergenic 35.0%
CCTTCGAAGTCAAAAATATG+CGG - chr4_2:51145220-51145239 Msa0605310:intron 35.0%
TTAGACCTTATACTCAACCA+AGG - chr4_2:51145414-51145433 Msa0605310:intron 35.0%
ATGCAATGATGCATAATCCT+TGG + chr4_2:51145434-51145453 None:intergenic 35.0%
TCAGTTTACGATGTTACCAT+TGG - chr4_2:51145645-51145664 Msa0605310:intron 35.0%
ATACATTGTCCAAGAAAGAC+GGG + chr4_2:51145686-51145705 None:intergenic 35.0%
ATATGGATATGAACTTCGGA+AGG + chr4_2:51145720-51145739 None:intergenic 35.0%
GCAGATATGGATATGAACTT+CGG + chr4_2:51145724-51145743 None:intergenic 35.0%
CTGAAGATGAAATTTCTACG+TGG - chr4_2:51145772-51145791 Msa0605310:intron 35.0%
TGAAATTTCTACGTGGTTGA+TGG - chr4_2:51145779-51145798 Msa0605310:intron 35.0%
GATGGATAGATTCCGATTTA+AGG - chr4_2:51145797-51145816 Msa0605310:intron 35.0%
GTGACAGTGATTATATTGAC+TGG - chr4_2:51145912-51145931 Msa0605310:intron 35.0%
GTAAAGTTTAAACCAGACAG+AGG + chr4_2:51145961-51145980 None:intergenic 35.0%
CGAATGTTGAAATATGTAGC+CGG + chr4_2:51146011-51146030 None:intergenic 35.0%
CGGCTACATATTTCAACATT+CGG - chr4_2:51146009-51146028 Msa0605310:five_prime_UTR 35.0%
TGAAACATTCTCCAACATCA+GGG + chr4_2:51146180-51146199 None:intergenic 35.0%
ATGAAACATTCTCCAACATC+AGG + chr4_2:51146181-51146200 None:intergenic 35.0%
!! GTGCAACTTTGAAACAGTTG+TGG - chr4_2:51142531-51142550 Msa0605310:three_prime_UTR 40.0%
!! CAACTTTGAAACAGTTGTGG+AGG - chr4_2:51142534-51142553 Msa0605310:three_prime_UTR 40.0%
! AAGTGAGGTGTAGATTTTGG+TGG + chr4_2:51142614-51142633 None:intergenic 40.0%
AAGACTAAAGATCCGAGCTT+TGG - chr4_2:51142905-51142924 Msa0605310:CDS 40.0%
! ATGTCTTCGATCTGAAGTGT+CGG + chr4_2:51143266-51143285 None:intergenic 40.0%
!! TGTTGCATGTCTTTAGGATG+TGG - chr4_2:51143507-51143526 Msa0605310:CDS 40.0%
! TACTCACTCTTCGATCCTTT+TGG - chr4_2:51143847-51143866 Msa0605310:intron 40.0%
GATATGCAGAACAAACTCCA+AGG - chr4_2:51144147-51144166 Msa0605310:CDS 40.0%
ACGCGTGTAACTTCAAAACT+TGG - chr4_2:51144275-51144294 Msa0605310:CDS 40.0%
!!! CGTCGTTTTTCTTTGGTACT+TGG - chr4_2:51144453-51144472 Msa0605310:CDS 40.0%
TTGGTACTTGGCTAACTTTG+TGG - chr4_2:51144465-51144484 Msa0605310:exon 40.0%
ACGTAAAATCAGTGCCTTCA+GGG + chr4_2:51144817-51144836 None:intergenic 40.0%
TACGTAAAATCAGTGCCTTC+AGG + chr4_2:51144818-51144837 None:intergenic 40.0%
CAAAGTCACCATTCGAAGTA+TGG + chr4_2:51145078-51145097 None:intergenic 40.0%
GATGGCTAAGAATCAGCAAA+AGG + chr4_2:51145169-51145188 None:intergenic 40.0%
!!! CCGCATATTTTTGACTTCGA+AGG + chr4_2:51145223-51145242 None:intergenic 40.0%
CAAAAATATGCGGCTGAACA+CGG - chr4_2:51145230-51145249 Msa0605310:intron 40.0%
AAAAATATGCGGCTGAACAC+GGG - chr4_2:51145231-51145250 Msa0605310:intron 40.0%
! TGTTTTACTGCCGAAAACCA+AGG - chr4_2:51145271-51145290 Msa0605310:intron 40.0%
! GTTTTACTGCCGAAAACCAA+GGG - chr4_2:51145272-51145291 Msa0605310:intron 40.0%
!! GTACAAAAGCCCTTGGTTTT+CGG + chr4_2:51145284-51145303 None:intergenic 40.0%
CTTGGACAATGTATTTGGAG+TGG - chr4_2:51145692-51145711 Msa0605310:intron 40.0%
! CTTTTGCTTGATCAGGAAAC+TGG + chr4_2:51145854-51145873 None:intergenic 40.0%
TTCCTGATCAAGCAAAAGAG+AGG - chr4_2:51145856-51145875 Msa0605310:intron 40.0%
TCCTGATCAAGCAAAAGAGA+GGG - chr4_2:51145857-51145876 Msa0605310:intron 40.0%
TTAAAATGCCCTTCACAGCA+GGG + chr4_2:51145887-51145906 None:intergenic 40.0%
! TTTAAAATGCCCTTCACAGC+AGG + chr4_2:51145888-51145907 None:intergenic 40.0%
!!! ACTTCACTTTTTCCTCTGTC+TGG - chr4_2:51145946-51145965 Msa0605310:intron 40.0%
!! AAAACTCCAAGAATGGGTTG+TGG + chr4_2:51146035-51146054 None:intergenic 40.0%
AGGGCTAAGGAAACAATCTT+CGG + chr4_2:51146161-51146180 None:intergenic 40.0%
GGAAGGCTATTGCAATTCAG+AGG + chr4_2:51143773-51143792 None:intergenic 45.0%
ATACGATAGATCGCACCTAG+GGG + chr4_2:51144097-51144116 None:intergenic 45.0%
GATACGATAGATCGCACCTA+GGG + chr4_2:51144098-51144117 None:intergenic 45.0%
TGATACGATAGATCGCACCT+AGG + chr4_2:51144099-51144118 None:intergenic 45.0%
ATGTACAAGGACAAAGCCAC+TGG - chr4_2:51144118-51144137 Msa0605310:CDS 45.0%
ACAAGCCTGGATTGACAGAA+TGG + chr4_2:51144200-51144219 None:intergenic 45.0%
!! AAGGGGTTTGGTGTGTCTAT+TGG - chr4_2:51144334-51144353 Msa0605310:CDS 45.0%
! TTTGGTGTGTCTATTGGTGC+TGG - chr4_2:51144340-51144359 Msa0605310:CDS 45.0%
! ACATCCAGTCGACTTCAGTT+CGG + chr4_2:51144583-51144602 None:intergenic 45.0%
ATTTATGGGACCTTGCGTTG+CGG - chr4_2:51144603-51144622 Msa0605310:five_prime_UTR 45.0%
CATACTAGAGTTCATCCCAG+TGG - chr4_2:51144703-51144722 Msa0605310:intron 45.0%
TAGAGTTCATCCCAGTGGAA+AGG - chr4_2:51144708-51144727 Msa0605310:intron 45.0%
!!! TGGCTTGCTATTTTCCCTGA+AGG - chr4_2:51144800-51144819 Msa0605310:intron 45.0%
GCTTATGATCATCGTGATGC+TGG - chr4_2:51144969-51144988 Msa0605310:intron 45.0%
GCTATCACCCATACTTCGAA+TGG - chr4_2:51145067-51145086 Msa0605310:intron 45.0%
CAAACAGGTACAAAAGCCCT+TGG + chr4_2:51145291-51145310 None:intergenic 45.0%
GATGTTGGGATACAGTGGAT+GGG + chr4_2:51145753-51145772 None:intergenic 45.0%
AGATGTTGGGATACAGTGGA+TGG + chr4_2:51145754-51145773 None:intergenic 45.0%
CTTCAGATGTTGGGATACAG+TGG + chr4_2:51145758-51145777 None:intergenic 45.0%
! TCCCTCTCTTTTGCTTGATC+AGG + chr4_2:51145861-51145880 None:intergenic 45.0%
GTCTGTAGTCGTCGCTTATT+TGG - chr4_2:51145983-51146002 Msa0605310:five_prime_UTR 45.0%
! AGTCGTCGCTTATTTGGTTC+CGG - chr4_2:51145989-51146008 Msa0605310:five_prime_UTR 45.0%
TGTTTCCTTAGCCCTGATGT+TGG - chr4_2:51146166-51146185 Msa0605310:five_prime_UTR 45.0%
ATTCTCCAACATCAGGGCTA+AGG + chr4_2:51146174-51146193 None:intergenic 45.0%
TCGGACTGTCGGTACTACAT+AGG + chr4_2:51143247-51143266 None:intergenic 50.0%
!! GATCTGAAGTGTCGGACTGT+CGG + chr4_2:51143258-51143277 None:intergenic 50.0%
CATCCAGTCTACTTTCCCCT+AGG - chr4_2:51144079-51144098 Msa0605310:CDS 50.0%
TCTGCATATCACTCTGCCAG+TGG + chr4_2:51144137-51144156 None:intergenic 50.0%
AGCACAATCCGTTCTCTCGA+AGG + chr4_2:51144548-51144567 None:intergenic 50.0%
ATCACCGAACTGAAGTCGAC+TGG - chr4_2:51144576-51144595 Msa0605310:five_prime_UTR 50.0%
TCATCCCAGTGGAAAGGAAG+TGG - chr4_2:51144714-51144733 Msa0605310:intron 50.0%
CATCCCAGTGGAAAGGAAGT+GGG - chr4_2:51144715-51144734 Msa0605310:intron 50.0%
CTTCCCACTTCCTTTCCACT+GGG + chr4_2:51144721-51144740 None:intergenic 50.0%
ATGATCCCCAAGATCCTCTC+TGG - chr4_2:51144780-51144799 Msa0605310:intron 50.0%
AGCAAGCCAGAGAGGATCTT+GGG + chr4_2:51144789-51144808 None:intergenic 50.0%
TAGCAAGCCAGAGAGGATCT+TGG + chr4_2:51144790-51144809 None:intergenic 50.0%
GGGAAAATAGCAAGCCAGAG+AGG + chr4_2:51144797-51144816 None:intergenic 50.0%
GTGATGCTGGAGAAGTAGTC+AGG - chr4_2:51144982-51145001 Msa0605310:intron 50.0%
ACCTGTTTGCAAGAGTTGCG+AGG - chr4_2:51145302-51145321 Msa0605310:intron 50.0%
TTGCGAGGCTCTCTAAATGC+AGG - chr4_2:51145317-51145336 Msa0605310:intron 50.0%
GAGGCTCTCTAAATGCAGGT+TGG - chr4_2:51145321-51145340 Msa0605310:intron 50.0%
GCAGCGGTATTTGTAGCCAA+TGG + chr4_2:51145664-51145683 None:intergenic 50.0%
GGATACAGTGGATGGGAAAG+CGG + chr4_2:51145746-51145765 None:intergenic 50.0%
ATTCGGCCACAACCCATTCT+TGG - chr4_2:51146026-51146045 Msa0605310:five_prime_UTR 50.0%
GCACCTAGGGGAAAGTAGAC+TGG + chr4_2:51144085-51144104 None:intergenic 55.0%
GCTTCCCACTTCCTTTCCAC+TGG + chr4_2:51144722-51144741 None:intergenic 55.0%
GCAAGCCAGAGAGGATCTTG+GGG + chr4_2:51144788-51144807 None:intergenic 55.0%
GCCTCGCAACTCTTGCAAAC+AGG + chr4_2:51145306-51145325 None:intergenic 55.0%
ATACCGCTGCCCGTCTTTCT+TGG - chr4_2:51145674-51145693 Msa0605310:intron 55.0%
TGTCCAAGAAAGACGGGCAG+CGG + chr4_2:51145680-51145699 None:intergenic 55.0%
GGATGGGAAAGCGGCAGATA+TGG + chr4_2:51145737-51145756 None:intergenic 55.0%
AAATGCCCTTCACAGCAGGG+AGG + chr4_2:51145884-51145903 None:intergenic 55.0%
ATGGGACCTTGCGTTGCGGA+AGG - chr4_2:51144607-51144626 Msa0605310:five_prime_UTR 60.0%
TGGGACCTTGCGTTGCGGAA+GGG - chr4_2:51144608-51144627 Msa0605310:five_prime_UTR 60.0%
TGCATCCCTTCCGCAACGCA+AGG + chr4_2:51144616-51144635 None:intergenic 60.0%
GCGTTGCGGAAGGGATGCAT+TGG - chr4_2:51144617-51144636 Msa0605310:five_prime_UTR 60.0%
AGGGACCTCCCTGCTGTGAA+GGG - chr4_2:51145876-51145895 Msa0605310:intron 60.0%
GAGGGACCTCCCTGCTGTGA+AGG - chr4_2:51145875-51145894 Msa0605310:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51142522 51146217 51142522 ID=Msa0605310;Name=Msa0605310
chr4_2 mRNA 51142522 51146217 51142522 ID=Msa0605310-mRNA-1;Parent=Msa0605310;Name=Msa0605310-mRNA-1;_AED=0.05;_eAED=0.05;_QI=456|1|1|1|0.66|0.5|4|153|372
chr4_2 exon 51142522 51143090 51142522 ID=Msa0605310-mRNA-1:exon:12220;Parent=Msa0605310-mRNA-1
chr4_2 exon 51143401 51143531 51143401 ID=Msa0605310-mRNA-1:exon:12219;Parent=Msa0605310-mRNA-1
chr4_2 exon 51143905 51144667 51143905 ID=Msa0605310-mRNA-1:exon:12218;Parent=Msa0605310-mRNA-1
chr4_2 exon 51145953 51146217 51145953 ID=Msa0605310-mRNA-1:exon:12217;Parent=Msa0605310-mRNA-1
chr4_2 five_prime_UTR 51145953 51146217 51145953 ID=Msa0605310-mRNA-1:five_prime_utr;Parent=Msa0605310-mRNA-1
chr4_2 five_prime_UTR 51144477 51144667 51144477 ID=Msa0605310-mRNA-1:five_prime_utr;Parent=Msa0605310-mRNA-1
chr4_2 CDS 51143905 51144476 51143905 ID=Msa0605310-mRNA-1:cds;Parent=Msa0605310-mRNA-1
chr4_2 CDS 51143401 51143531 51143401 ID=Msa0605310-mRNA-1:cds;Parent=Msa0605310-mRNA-1
chr4_2 CDS 51142675 51143090 51142675 ID=Msa0605310-mRNA-1:cds;Parent=Msa0605310-mRNA-1
chr4_2 three_prime_UTR 51142522 51142674 51142522 ID=Msa0605310-mRNA-1:three_prime_utr;Parent=Msa0605310-mRNA-1
Gene Sequence

>Msa0605310

ATGGCGGTTCTTACGCGTGTAACTTCAAAACTTGGAGCAAAGTATCGTGCTCTAGCTCCTTTTAGATTTCTAAGGGGTTTGGTGTGTCTATTGGTGCTGGTTTCATCAGCATTTGTAACATTGGTGTTCTTCGGTTTTATTAGTTCTGTTATAATGCGATTTTTTAGCATCAGTTACAGCAGGAAAACAACGTCGTTTTTCTTTGGTACTTGGCTAACTTTGTGGCCCTTTCTATTTGAAAAAATCAACAAGACTAAAGTTGTGTTCTCTGGAGATATTGTTCCTTCGAGAGAACGGATTGTGCTGATCGCAAATCACCGAACTGAAGTCGACTGGATGTATTTATGGGACCTTGCGTTGCGGAAGGGATGCATTGGATACATTAAATATGTACTTAAGAGCAGCTTGATGAGACTTCCAATTTTTGGTTGGGCATTTCACATACTAGAGTTCATCCCAGTGGAAAGGAAGTGGGAAGCTGATGAGTCAAATATGCGTCGAATGCTTTCTACATTCAATGATCCCCAAGATCCTCTCTGGCTTGCTATTTTCCCTGAAGGCACTGATTTTACTGAGCAGAAGTGCCTTCGAAGTCAAAAATATGCGGCTGAACACGGGTTACCGATTCTGAAAAATGTTTTACTGCCGAAAACCAAGGGCTTTTGTACCTGTTTGCAAGAGTTGCGAGGCTCTCTAAATGCAGTTTACGATGTTACCATTGGCTACAAATACCGCTGCCCGTCTTTCTTGGACAATGTATTTGGAGTGGATCCTTCCGAAGTTCATATCCATATCTGCCGCTTTCCCATCCACTGTATCCCAACATCTGAAGATGAAATTTCTACGTGGTTGATGGATAGATTCCGATTTAAGGACAAGTTGCTTTACAATTTTCAATTTCAAGGCCAGTTTCCTGATCAAGCAAAAGAGAGGGACCTCCCTGCTGTGAAGGGCATTTTAAATTGTGTGACAGTGATTATATTGACTGGCTTATGCATGTACTTCACTTTTTCCTCTGTCTGGTTTAAACTTTACGTGTCTGTAGTCGTCGCTTATTTGGTTCCGGCTACATATTTCAACATTCGGCCACAACCCATTCTTGGAGTTTTTAAGATGTGA
Protein sequence

>Msa0605310

MAVLTRVTSKLGAKYRALAPFRFLRGLVCLLVLVSSAFVTLVFFGFISSVIMRFFSISYSRKTTSFFFGTWLTLWPFLFEKINKTKVVFSGDIVPSRERIVLIANHRTEVDWMYLWDLALRKGCIGYIKYVLKSSLMRLPIFGWAFHILEFIPVERKWEADESNMRRMLSTFNDPQDPLWLAIFPEGTDFTEQKCLRSQKYAAEHGLPILKNVLLPKTKGFCTCLQELRGSLNAVYDVTIGYKYRCPSFLDNVFGVDPSEVHIHICRFPIHCIPTSEDEISTWLMDRFRFKDKLLYNFQFQGQFPDQAKERDLPAVKGILNCVTVIILTGLCMYFTFSSVWFKLYVSVVVAYLVPATYFNIRPQPILGVFKM*