AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0607250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607250 MtrunA17_Chr4g0038851 95.763 472 20 0 4 475 1 472 0.0 916
Msa0607250 MtrunA17_Chr5g0396491 74.948 479 112 6 4 475 4 481 0.0 732
Msa0607250 MtrunA17_Chr3g0085681 49.663 445 214 4 30 474 4 438 2.36e-160 461
Msa0607250 MtrunA17_Chr3g0085361 50.704 355 164 5 120 474 9 352 1.05e-125 369
Msa0607250 MtrunA17_Chr7g0224271 33.556 450 217 6 40 472 289 673 1.20e-73 244
Msa0607250 MtrunA17_Chr5g0447691 31.323 431 230 6 48 472 174 544 3.95e-66 222
Msa0607250 MtrunA17_Chr1g0159461 29.837 429 235 8 50 472 224 592 9.46e-56 195
Msa0607250 MtrunA17_Chr3g0125811 29.138 429 238 8 50 472 202 570 5.29e-54 190
Msa0607250 MtrunA17_Chr3g0101141 27.928 444 253 8 35 472 160 542 2.89e-52 184
Msa0607250 MtrunA17_Chr8g0357601 29.730 444 231 12 44 472 175 552 1.68e-50 180
Msa0607250 MtrunA17_Chr4g0057891 29.025 441 237 11 45 472 143 520 1.81e-49 177
Msa0607250 MtrunA17_Chr4g0031721 28.205 429 239 7 51 471 316 683 1.97e-49 179
Msa0607250 MtrunA17_Chr2g0306641 30.455 440 231 15 42 473 116 488 5.34e-48 172
Msa0607250 MtrunA17_Chr2g0290241 28.037 428 241 9 52 472 58 425 3.74e-47 168
Msa0607250 MtrunA17_Chr4g0031701 26.269 453 265 7 24 467 229 621 1.60e-46 171
Msa0607250 MtrunA17_Chr7g0238891 26.869 428 248 5 51 472 372 740 4.15e-46 171
Msa0607250 MtrunA17_Chr4g0031731 26.049 453 266 7 24 467 229 621 8.28e-45 166
Msa0607250 MtrunA17_Chr4g0031711 26.957 460 262 11 23 471 336 732 1.06e-43 164
Msa0607250 MtrunA17_Chr2g0328391 25.935 428 252 5 51 471 299 668 1.23e-43 163
Msa0607250 MtrunA17_Chr2g0328411 26.147 436 236 8 51 471 289 653 1.36e-40 154
Msa0607250 MtrunA17_Chr2g0328431 26.230 427 247 7 51 471 289 653 6.07e-40 152
Msa0607250 MtrunA17_Chr4g0057291 35.349 215 135 1 44 254 285 499 3.52e-38 147
Msa0607250 MtrunA17_Chr4g0057291 31.373 153 98 5 325 471 510 661 1.69e-18 88.6
Msa0607250 MtrunA17_Chr1g0200401 27.948 458 230 14 45 473 74 460 6.73e-38 144
Msa0607250 MtrunA17_Chr2g0294471 29.083 447 236 15 42 472 195 576 1.44e-37 145
Msa0607250 MtrunA17_Chr7g0245601 40.314 191 111 2 55 244 442 630 2.96e-37 145
Msa0607250 MtrunA17_Chr7g0245601 36.913 149 89 3 327 472 646 792 4.97e-20 93.6
Msa0607250 MtrunA17_Chr1g0183641 27.689 437 239 12 43 474 115 479 4.89e-37 142
Msa0607250 MtrunA17_Chr2g0294461 27.716 451 240 14 42 472 201 585 3.18e-36 141
Msa0607250 MtrunA17_Chr2g0328401 26.984 378 208 6 51 422 290 605 4.90e-36 141
Msa0607250 MtrunA17_Chr3g0110971 34.685 222 131 4 39 255 207 419 1.56e-34 136
Msa0607250 MtrunA17_Chr3g0110971 36.196 163 91 2 323 472 425 587 7.21e-28 117
Msa0607250 MtrunA17_Chr8g0376381 33.482 224 130 5 39 255 157 368 1.41e-31 127
Msa0607250 MtrunA17_Chr8g0376381 36.601 153 90 3 323 472 374 522 5.57e-28 116
Msa0607250 MtrunA17_Chr3g0114841 25.926 459 249 14 38 471 107 499 2.77e-31 126
Msa0607250 MtrunA17_Chr2g0319091 33.778 225 139 3 48 266 209 429 1.20e-30 125
Msa0607250 MtrunA17_Chr2g0319091 40.940 149 87 1 323 471 431 578 6.83e-29 119
Msa0607250 MtrunA17_Chr5g0445331 31.556 225 145 4 35 251 146 369 8.08e-30 122
Msa0607250 MtrunA17_Chr5g0445331 38.298 141 86 1 332 472 393 532 1.34e-25 109
Msa0607250 MtrunA17_Chr2g0328341 28.807 243 163 4 26 266 251 485 1.33e-29 122
Msa0607250 MtrunA17_Chr2g0328361 24.312 436 242 10 40 471 263 614 2.57e-29 121
Msa0607250 MtrunA17_Chr4g0031741 31.111 225 142 4 48 266 281 498 3.11e-29 121
Msa0607250 MtrunA17_Chr4g0031741 31.333 150 102 1 323 471 500 649 1.13e-15 79.7
Msa0607250 MtrunA17_Chr7g0270031 25.567 485 278 13 4 472 137 554 6.57e-29 119
Msa0607250 MtrunA17_Chr2g0328381 27.511 229 160 3 40 266 264 488 9.99e-29 119
Msa0607250 MtrunA17_Chr4g0038761 32.124 193 128 2 55 244 88 280 4.32e-26 110
Msa0607250 MtrunA17_Chr4g0038761 39.597 149 84 4 326 472 298 442 7.59e-20 92.0
Msa0607250 MtrunA17_Chr2g0328371 25.000 292 158 7 134 422 13 246 8.33e-26 105
Msa0607250 MtrunA17_Chr4g0053901 24.390 492 292 11 14 471 22 467 9.04e-26 109
Msa0607250 MtrunA17_Chr5g0401421 24.536 485 283 14 22 471 9 445 2.01e-25 108
Msa0607250 MtrunA17_Chr1g0207361 63.366 101 35 2 276 375 64 163 2.03e-25 102
Msa0607250 MtrunA17_Chr1g0207361 72.222 54 15 0 85 138 1 54 7.59e-22 92.4
Msa0607250 MtrunA17_Chr5g0424131 31.169 231 142 4 37 250 107 337 6.13e-25 107
Msa0607250 MtrunA17_Chr5g0424131 27.333 150 106 3 324 471 350 498 1.46e-11 66.6
Msa0607250 MtrunA17_Chr5g0401131 31.651 218 139 4 45 253 108 324 1.39e-24 106
Msa0607250 MtrunA17_Chr4g0076091 31.163 215 140 3 46 253 170 383 2.34e-24 106
Msa0607250 MtrunA17_Chr4g0076091 34.228 149 97 1 323 471 395 542 1.59e-21 97.4
Msa0607250 MtrunA17_Chr2g0329591 31.019 216 137 5 42 247 65 278 5.26e-22 98.2
Msa0607250 MtrunA17_Chr1g0194621 33.728 169 102 5 310 472 354 518 1.76e-21 97.4
Msa0607250 MtrunA17_Chr1g0194621 25.000 248 165 8 42 269 137 383 6.11e-12 67.8
Msa0607250 MtrunA17_Chr4g0039661 28.700 223 149 3 48 263 190 409 5.61e-20 92.8
Msa0607250 MtrunA17_Chr4g0039661 29.508 183 116 6 298 472 378 555 3.73e-13 71.6
Msa0607250 MtrunA17_Chr4g0012071 25.293 427 241 11 52 472 197 551 6.78e-20 92.4
Msa0607250 MtrunA17_Chr6g0468561 26.331 357 191 11 127 472 367 662 1.47e-19 91.7
Msa0607250 MtrunA17_Chr7g0236851 33.333 150 95 3 323 471 511 656 2.68e-19 90.9
Msa0607250 MtrunA17_Chr7g0230731 32.941 170 106 3 22 191 349 510 1.38e-14 76.3
Msa0607250 MtrunA17_Chr7g0230771 32.941 170 106 3 22 191 352 513 1.68e-14 76.3
Msa0607250 MtrunA17_Chr8g0383981 29.126 206 132 8 48 248 136 332 1.42e-13 72.8
Msa0607250 MtrunA17_Chr3g0087711 51.724 58 28 0 202 259 1 58 7.41e-13 63.2
Msa0607250 MtrunA17_Chr2g0323111 26.846 149 107 2 323 471 159 305 8.25e-13 69.3
Msa0607250 MtrunA17_Chr8g0344101 22.414 464 250 14 45 475 217 603 4.89e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0607250 AT1G50420.1 62.810 484 169 5 1 475 1 482 0.0 613
Msa0607250 AT1G66350.1 31.808 437 222 8 39 472 143 506 1.95e-67 225
Msa0607250 AT5G48150.1 30.679 427 231 5 52 472 123 490 5.79e-66 220
Msa0607250 AT5G48150.4 30.679 427 231 5 52 472 123 490 5.79e-66 220
Msa0607250 AT5G48150.2 30.679 427 231 5 52 472 123 490 5.79e-66 220
Msa0607250 AT5G48150.3 30.679 427 231 5 52 472 123 490 5.79e-66 220
Msa0607250 AT2G04890.1 32.938 422 222 5 51 472 53 413 2.17e-65 216
Msa0607250 AT5G17490.1 30.464 453 231 11 34 472 134 516 2.28e-59 204
Msa0607250 AT1G50600.3 29.322 457 251 10 27 472 131 526 1.80e-58 201
Msa0607250 AT1G50600.1 29.386 456 250 10 28 472 203 597 4.66e-58 202
Msa0607250 AT1G50600.2 29.322 457 251 10 27 472 206 601 5.05e-58 202
Msa0607250 AT1G21450.1 30.769 429 232 7 50 472 224 593 7.11e-56 196
Msa0607250 AT1G14920.1 30.112 445 225 12 39 472 160 529 9.52e-53 186
Msa0607250 AT2G01570.1 28.764 445 231 13 39 472 212 581 1.58e-47 173
Msa0607250 AT5G41920.1 30.023 433 227 11 48 474 40 402 9.24e-46 164
Msa0607250 AT1G07530.1 27.727 440 228 10 51 471 396 764 1.75e-43 164
Msa0607250 AT3G54220.1 42.180 211 120 1 36 244 278 488 5.92e-41 155
Msa0607250 AT3G54220.1 35.616 146 92 2 329 474 509 652 7.10e-20 93.2
Msa0607250 AT2G29060.2 26.959 434 247 10 48 471 281 654 2.06e-39 151
Msa0607250 AT2G29060.1 26.959 434 247 10 48 471 318 691 2.96e-39 151
Msa0607250 AT5G66770.1 28.899 436 231 12 48 472 217 584 6.62e-39 149
Msa0607250 AT1G07520.2 25.056 443 264 7 40 471 143 528 1.33e-38 147
Msa0607250 AT1G07520.4 25.056 443 264 7 40 471 143 528 1.33e-38 147
Msa0607250 AT1G07520.3 25.056 443 264 7 40 471 308 693 5.41e-38 147
Msa0607250 AT1G07520.1 25.056 443 264 7 40 471 307 692 6.70e-38 147
Msa0607250 AT5G59450.1 25.576 434 255 7 48 472 224 598 4.14e-37 144
Msa0607250 AT2G29065.1 25.342 438 248 10 51 471 252 627 2.49e-36 142
Msa0607250 AT3G46600.2 25.926 432 254 6 50 475 3 374 4.56e-36 137
Msa0607250 AT2G37650.1 24.594 431 258 6 48 472 344 713 1.43e-35 140
Msa0607250 AT2G37650.2 24.594 431 258 6 48 472 332 701 1.48e-35 140
Msa0607250 AT3G46600.3 25.926 432 254 6 50 475 179 550 8.61e-35 137
Msa0607250 AT3G46600.1 25.843 445 263 7 37 475 199 582 2.47e-34 136
Msa0607250 AT1G63100.2 32.218 239 157 4 43 277 269 506 2.20e-31 128
Msa0607250 AT1G63100.2 33.125 160 94 2 325 471 494 653 1.05e-20 95.9
Msa0607250 AT1G63100.1 32.218 239 157 4 43 277 244 481 2.91e-31 127
Msa0607250 AT1G63100.1 33.125 160 94 2 325 471 469 628 7.48e-21 96.3
Msa0607250 AT3G03450.1 31.839 223 143 5 39 255 171 390 2.84e-30 124
Msa0607250 AT3G03450.1 38.854 157 94 2 317 472 390 545 1.72e-28 119
Msa0607250 AT5G52510.1 26.376 436 246 13 50 472 267 640 3.17e-30 124
Msa0607250 AT1G55580.1 38.255 149 91 1 325 472 297 445 6.39e-28 115
Msa0607250 AT1G55580.1 27.843 255 143 8 47 261 40 293 5.63e-13 71.2
Msa0607250 AT4G37650.1 31.984 247 138 7 24 253 131 364 3.20e-24 105
Msa0607250 AT3G50650.1 26.157 432 235 13 56 472 180 542 4.88e-24 105
Msa0607250 AT4G17230.1 34.000 150 98 1 323 472 377 525 7.43e-23 102
Msa0607250 AT4G17230.1 29.245 212 143 2 46 251 153 363 1.68e-22 100
Msa0607250 AT4G17230.2 34.000 150 98 1 323 472 377 525 7.43e-23 102
Msa0607250 AT4G17230.2 29.245 212 143 2 46 251 153 363 1.68e-22 100
Msa0607250 AT4G08250.1 30.116 259 153 9 6 250 72 316 2.81e-21 97.1
Msa0607250 AT3G49950.1 22.343 461 275 14 29 471 12 407 2.91e-16 81.3
Msa0607250 AT4G00150.1 26.667 195 134 5 283 474 368 556 2.42e-11 66.2

Find 128 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4_2:+54022473 Msa0607250:CDS
CCGAATATGCTTCGGTTTAC+TGG 0.158109 4_2:-54022193 None:intergenic
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4_2:+54022619 Msa0607250:CDS
TTCTTCCTCAGTCATGATTT+TGG 0.198283 4_2:+54021019 Msa0607250:exon
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4_2:-54022105 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4_2:+54022435 Msa0607250:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4_2:+54022006 Msa0607250:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4_2:-54021396 None:intergenic
CAGAACAAAGTAACTCATTA+AGG 0.282442 4_2:+54020891 Msa0607250:five_prime_UTR
AGATTTGATTTAGCTGGATT+TGG 0.318965 4_2:+54022595 Msa0607250:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4_2:+54022749 Msa0607250:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4_2:-54022469 None:intergenic
AGAGTGTGAGTTTGAACTTT+TGG 0.331382 4_2:-54020845 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4_2:+54021416 Msa0607250:CDS
CCAGTAAACCGAAGCATATT+CGG 0.339215 4_2:+54022193 Msa0607250:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4_2:+54021763 Msa0607250:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4_2:+54021410 Msa0607250:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4_2:-54022625 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4_2:-54021662 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4_2:+54022474 Msa0607250:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4_2:-54022000 None:intergenic
TTTGGACGAGCACTTGAGTT+TGG 0.364682 4_2:+54022791 Msa0607250:three_prime_UTR
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4_2:+54022216 Msa0607250:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4_2:+54021439 Msa0607250:CDS
GAGTTACTTTGTTCTGTTGT+TGG 0.378375 4_2:-54020884 None:intergenic
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4_2:+54022688 Msa0607250:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4_2:-54022251 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4_2:-54022468 None:intergenic
ACAAGTATGCAATTCCTTGA+TGG 0.395999 4_2:-54020996 None:intergenic
CAAATCCAAGAGGCAAGCTT+TGG 0.398712 4_2:+54020929 Msa0607250:five_prime_UTR
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4_2:-54021890 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4_2:+54022043 Msa0607250:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4_2:-54021871 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4_2:+54021624 Msa0607250:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4_2:-54022033 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4_2:+54021432 Msa0607250:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4_2:-54021670 None:intergenic
TTCAATTTCAAAACCCATCA+AGG 0.420097 4_2:+54020982 Msa0607250:five_prime_UTR
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4_2:-54022280 None:intergenic
CGCTGCATAGTATCGCCATC+AGG 0.429354 4_2:-54021587 None:intergenic
TGGTAATATGCTGGCAAGAT+AGG 0.431510 4_2:+54022716 Msa0607250:CDS
AGTAAGACGATGTGATTGTT+TGG 0.435895 4_2:+54022773 Msa0607250:exon
TTTATGAGATTTGATTTAGC+TGG 0.436460 4_2:+54022589 Msa0607250:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4_2:-54022093 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4_2:+54022493 Msa0607250:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4_2:+54022655 Msa0607250:CDS
CAAGTATGCAATTCCTTGAT+GGG 0.447226 4_2:-54020995 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4_2:+54021460 Msa0607250:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4_2:-54022113 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4_2:+54022370 Msa0607250:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4_2:-54022349 None:intergenic
AAATTCCAAAGCTTGCCTCT+TGG 0.452365 4_2:-54020934 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4_2:+54022558 Msa0607250:CDS
TCCATGCTTGCTTCCCCTGA+TGG 0.459836 4_2:+54021572 Msa0607250:CDS
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4_2:-54022104 None:intergenic
GCAAATTGCCGAATATGCTT+CGG 0.476281 4_2:-54022201 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4_2:-54021520 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4_2:-54021496 None:intergenic
ATATGCTTCGGTTTACTGGA+TGG 0.504223 4_2:-54022189 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4_2:-54021749 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4_2:+54021644 Msa0607250:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4_2:-54022161 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4_2:-54021748 None:intergenic
TTACTTTGTTCTGTTGTTGG+TGG 0.520167 4_2:-54020881 None:intergenic
TCAGGCTATTATTGAAGCAA+TGG 0.520201 4_2:+54021781 Msa0607250:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4_2:-54021411 None:intergenic
GCCATCAGGGGAAGCAAGCA+TGG 0.523131 4_2:-54021573 None:intergenic
CAAGAACATTATTGCATGTG+AGG 0.529680 4_2:+54022525 Msa0607250:CDS
TCGAATGGAATTCACTTACA+AGG 0.530422 4_2:+54022163 Msa0607250:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4_2:+54022044 Msa0607250:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4_2:+54022566 Msa0607250:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4_2:-54022259 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4_2:+54022540 Msa0607250:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4_2:-54021627 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4_2:+54022444 Msa0607250:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4_2:-54022062 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4_2:+54022261 Msa0607250:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4_2:+54021459 Msa0607250:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4_2:-54021449 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4_2:+54022678 Msa0607250:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4_2:+54021351 Msa0607250:intron
CCTCCACATTTGAGAATCAC+GGG 0.549738 4_2:+54021899 Msa0607250:CDS
CCCTCCACATTTGAGAATCA+CGG 0.550882 4_2:+54021898 Msa0607250:CDS
CCAAGCTGATATTGAAAACA+AGG 0.551538 4_2:-54022740 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4_2:-54021443 None:intergenic
AGAAGTGAAATCCACTGAGC+AGG 0.552331 4_2:-54021848 None:intergenic
CAATCAGTCTATGAGCTACC+TGG 0.553683 4_2:-54021949 None:intergenic
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4_2:+54021931 Msa0607250:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4_2:-54021448 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4_2:-54022408 None:intergenic
GCTATTATTGAAGCAATGGA+AGG 0.565201 4_2:+54021785 Msa0607250:CDS
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4_2:+54022477 Msa0607250:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4_2:+54022526 Msa0607250:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4_2:+54021350 Msa0607250:intron
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4_2:-54021422 None:intergenic
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4_2:+54022339 Msa0607250:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4_2:-54021995 None:intergenic
ATGGCTGTGTGGTAATATGC+TGG 0.583884 4_2:+54022707 Msa0607250:CDS
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4_2:+54022663 Msa0607250:CDS
GGGCGTTCATCAGAAGAAAG+AGG 0.588411 4_2:+54021919 Msa0607250:CDS
GCTGCATAGTATCGCCATCA+GGG 0.588825 4_2:-54021586 None:intergenic
TACAAAATACTTTCATACAA+AGG 0.591122 4_2:-54022935 None:intergenic
CAACTCTACTCGAGTGTCGA+TGG 0.592320 4_2:+54021682 Msa0607250:CDS
ACTTGAGTTTGGCAAAATTG+CGG 0.593940 4_2:+54022802 Msa0607250:three_prime_UTR
TATTATTGAAGCAATGGAAG+GGG 0.594602 4_2:+54021787 Msa0607250:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4_2:+54022638 Msa0607250:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4_2:+54021639 Msa0607250:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4_2:+54022317 Msa0607250:CDS
CATCACTCTTCAAATCCAAG+AGG 0.605474 4_2:+54020919 Msa0607250:five_prime_UTR
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4_2:+54021454 Msa0607250:CDS
CCCGTGATTCTCAAATGTGG+AGG 0.607231 4_2:-54021899 None:intergenic
CTATTATTGAAGCAATGGAA+GGG 0.615371 4_2:+54021786 Msa0607250:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4_2:+54022333 Msa0607250:CDS
TGAGGCATATAGAATGAAGG+AGG 0.622680 4_2:+54022681 Msa0607250:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4_2:+54022545 Msa0607250:CDS
CCGTGATTCTCAAATGTGGA+GGG 0.629069 4_2:-54021898 None:intergenic
TGAGTTTGGCAAAATTGCGG+TGG 0.634786 4_2:+54022805 Msa0607250:three_prime_UTR
ACGCCCGTGATTCTCAAATG+TGG 0.636143 4_2:-54021902 None:intergenic
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4_2:+54021837 Msa0607250:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4_2:-54021419 None:intergenic
CTGCATAGTATCGCCATCAG+GGG 0.660723 4_2:-54021585 None:intergenic
CAGCGAGATTGACAAAATCA+CGG 0.671324 4_2:-54022832 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4_2:+54022045 Msa0607250:CDS
ACATACCAAAATCATGACTG+AGG 0.689238 4_2:-54021024 None:intergenic
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4_2:+54021461 Msa0607250:CDS
GAAGGAGGAAAATGGCTGTG+TGG 0.719265 4_2:+54022696 Msa0607250:CDS
CTGATGGCGATACTATGCAG+CGG 0.726536 4_2:+54021588 Msa0607250:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4_2:+54022048 Msa0607250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAGAAAAAAAAAATAA+AGG + chr4_2:54022892-54022911 Msa0607250:three_prime_UTR 10.0%
!! CAATAAGAAAAAAAAAATAA+AGG + chr4_2:54022892-54022911 Msa0607250:three_prime_UTR 10.0%
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_2:54022886-54022905 None:intergenic 15.0%
!!! TTTTTTTCTTATTGTCATTA+TGG - chr4_2:54022886-54022905 None:intergenic 15.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_2:54021329-54021348 Msa0607250:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_2:54022938-54022957 None:intergenic 20.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_2:54021329-54021348 Msa0607250:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_2:54022938-54022957 None:intergenic 20.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_2:54021089-54021108 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_2:54022444-54022463 Msa0607250:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_2:54022589-54022608 Msa0607250:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_2:54022628-54022647 None:intergenic 25.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_2:54021089-54021108 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_2:54022444-54022463 Msa0607250:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_2:54022589-54022608 Msa0607250:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_2:54022628-54022647 None:intergenic 25.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_2:54020891-54020910 Msa0607250:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_2:54020982-54021001 Msa0607250:five_prime_UTR 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_2:54021051-54021070 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_2:54021097-54021116 Msa0607250:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_2:54021238-54021257 Msa0607250:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_2:54021751-54021770 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_2:54021752-54021771 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_2:54021786-54021805 Msa0607250:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_2:54021787-54021806 Msa0607250:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_2:54022131-54022150 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_2:54022148-54022167 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_2:54022148-54022167 Msa0607250:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_2:54022216-54022235 Msa0607250:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_2:54022283-54022302 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_2:54022370-54022389 Msa0607250:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_2:54022384-54022403 Msa0607250:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_2:54022474-54022493 Msa0607250:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_2:54022526-54022545 Msa0607250:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_2:54022595-54022614 Msa0607250:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_2:54022619-54022638 Msa0607250:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_2:54022688-54022707 Msa0607250:CDS 30.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_2:54020891-54020910 Msa0607250:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_2:54020982-54021001 Msa0607250:five_prime_UTR 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_2:54021051-54021070 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_2:54021097-54021116 Msa0607250:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_2:54021238-54021257 Msa0607250:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_2:54021751-54021770 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_2:54021752-54021771 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_2:54021786-54021805 Msa0607250:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_2:54021787-54021806 Msa0607250:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_2:54022131-54022150 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_2:54022148-54022167 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_2:54022148-54022167 Msa0607250:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_2:54022216-54022235 Msa0607250:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_2:54022283-54022302 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_2:54022370-54022389 Msa0607250:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_2:54022384-54022403 Msa0607250:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_2:54022474-54022493 Msa0607250:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_2:54022526-54022545 Msa0607250:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_2:54022595-54022614 Msa0607250:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_2:54022619-54022638 Msa0607250:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_2:54022688-54022707 Msa0607250:CDS 30.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_2:54020848-54020867 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_2:54020884-54020903 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_2:54020887-54020906 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_2:54020941-54020960 Msa0607250:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_2:54020998-54021017 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_2:54020999-54021018 None:intergenic 35.0%
! TTCTTCCTCAGTCATGATTT+TGG + chr4_2:54021019-54021038 Msa0607250:exon 35.0%
ACATACCAAAATCATGACTG+AGG - chr4_2:54021027-54021046 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_2:54021101-54021120 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_2:54021451-54021470 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_2:54021639-54021658 Msa0607250:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_2:54021763-54021782 Msa0607250:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_2:54021781-54021800 Msa0607250:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_2:54021785-54021804 Msa0607250:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_2:54021998-54022017 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_2:54022013-54022032 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_2:54022036-54022055 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_2:54022043-54022062 Msa0607250:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_2:54022044-54022063 Msa0607250:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_2:54022164-54022183 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_2:54022163-54022182 Msa0607250:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_2:54022317-54022336 Msa0607250:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_2:54022333-54022352 Msa0607250:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_2:54022435-54022454 Msa0607250:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_2:54022471-54022490 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_2:54022472-54022491 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_2:54022473-54022492 Msa0607250:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_2:54022525-54022544 Msa0607250:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_2:54022540-54022559 Msa0607250:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_2:54022545-54022564 Msa0607250:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_2:54022678-54022697 Msa0607250:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_2:54022743-54022762 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_2:54022740-54022759 Msa0607250:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_2:54022773-54022792 Msa0607250:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_2:54022802-54022821 Msa0607250:three_prime_UTR 35.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_2:54020848-54020867 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_2:54020884-54020903 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_2:54020887-54020906 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_2:54020941-54020960 Msa0607250:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_2:54020998-54021017 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_2:54020999-54021018 None:intergenic 35.0%
! TTCTTCCTCAGTCATGATTT+TGG + chr4_2:54021019-54021038 Msa0607250:exon 35.0%
ACATACCAAAATCATGACTG+AGG - chr4_2:54021027-54021046 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_2:54021101-54021120 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_2:54021451-54021470 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_2:54021639-54021658 Msa0607250:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_2:54021763-54021782 Msa0607250:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_2:54021781-54021800 Msa0607250:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_2:54021785-54021804 Msa0607250:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_2:54021998-54022017 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_2:54022013-54022032 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_2:54022036-54022055 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_2:54022043-54022062 Msa0607250:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_2:54022044-54022063 Msa0607250:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_2:54022164-54022183 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_2:54022163-54022182 Msa0607250:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_2:54022317-54022336 Msa0607250:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_2:54022333-54022352 Msa0607250:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_2:54022435-54022454 Msa0607250:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_2:54022471-54022490 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_2:54022472-54022491 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_2:54022473-54022492 Msa0607250:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_2:54022525-54022544 Msa0607250:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_2:54022540-54022559 Msa0607250:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_2:54022545-54022564 Msa0607250:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_2:54022678-54022697 Msa0607250:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_2:54022743-54022762 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_2:54022740-54022759 Msa0607250:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_2:54022773-54022792 Msa0607250:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_2:54022802-54022821 Msa0607250:three_prime_UTR 35.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_2:54020919-54020938 Msa0607250:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_2:54020937-54020956 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_2:54021208-54021227 Msa0607250:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_2:54021351-54021370 Msa0607250:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_2:54021399-54021418 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_2:54021452-54021471 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_2:54021454-54021473 Msa0607250:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_2:54021459-54021478 Msa0607250:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_2:54021460-54021479 Msa0607250:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_2:54021461-54021480 Msa0607250:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_2:54021499-54021518 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_2:54021523-54021542 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_2:54021630-54021649 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_2:54022003-54022022 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_2:54022006-54022025 Msa0607250:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_2:54022045-54022064 Msa0607250:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_2:54022096-54022115 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_2:54022095-54022114 Msa0607250:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_2:54022096-54022115 Msa0607250:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_2:54022107-54022126 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_2:54022108-54022127 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_2:54022192-54022211 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_2:54022193-54022212 Msa0607250:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_2:54022204-54022223 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_2:54022254-54022273 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_2:54022261-54022280 Msa0607250:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_2:54022339-54022358 Msa0607250:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_2:54022352-54022371 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_2:54022493-54022512 Msa0607250:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_2:54022498-54022517 Msa0607250:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_2:54022558-54022577 Msa0607250:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_2:54022638-54022657 Msa0607250:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_2:54022655-54022674 Msa0607250:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_2:54022681-54022700 Msa0607250:CDS 40.0%
TGGTAATATGCTGGCAAGAT+AGG + chr4_2:54022716-54022735 Msa0607250:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_2:54022749-54022768 Msa0607250:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_2:54022835-54022854 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_2:54022867-54022886 None:intergenic 40.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_2:54020919-54020938 Msa0607250:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_2:54020937-54020956 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_2:54021208-54021227 Msa0607250:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_2:54021351-54021370 Msa0607250:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_2:54021399-54021418 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_2:54021452-54021471 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_2:54021454-54021473 Msa0607250:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_2:54021459-54021478 Msa0607250:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_2:54021460-54021479 Msa0607250:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_2:54021461-54021480 Msa0607250:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_2:54021499-54021518 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_2:54021523-54021542 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_2:54021630-54021649 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_2:54022003-54022022 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_2:54022006-54022025 Msa0607250:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_2:54022045-54022064 Msa0607250:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_2:54022096-54022115 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_2:54022095-54022114 Msa0607250:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_2:54022096-54022115 Msa0607250:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_2:54022107-54022126 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_2:54022108-54022127 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_2:54022192-54022211 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_2:54022193-54022212 Msa0607250:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_2:54022204-54022223 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_2:54022254-54022273 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_2:54022261-54022280 Msa0607250:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_2:54022339-54022358 Msa0607250:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_2:54022352-54022371 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_2:54022493-54022512 Msa0607250:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_2:54022498-54022517 Msa0607250:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_2:54022558-54022577 Msa0607250:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_2:54022638-54022657 Msa0607250:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_2:54022655-54022674 Msa0607250:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_2:54022681-54022700 Msa0607250:CDS 40.0%
TGGTAATATGCTGGCAAGAT+AGG + chr4_2:54022716-54022735 Msa0607250:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_2:54022749-54022768 Msa0607250:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_2:54022835-54022854 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_2:54022867-54022886 None:intergenic 40.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_2:54020929-54020948 Msa0607250:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_2:54021350-54021369 Msa0607250:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_2:54021624-54021643 Msa0607250:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_2:54021644-54021663 Msa0607250:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_2:54021665-54021684 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_2:54021673-54021692 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_2:54021851-54021870 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_2:54021874-54021893 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_2:54021875-54021894 Msa0607250:CDS 45.0%
CCGTGATTCTCAAATGTGGA+GGG - chr4_2:54021901-54021920 None:intergenic 45.0%
CCCTCCACATTTGAGAATCA+CGG + chr4_2:54021898-54021917 Msa0607250:CDS 45.0%
CCTCCACATTTGAGAATCAC+GGG + chr4_2:54021899-54021918 Msa0607250:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_2:54021952-54021971 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_2:54022116-54022135 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_2:54022196-54022215 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_2:54022262-54022281 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_2:54022389-54022408 Msa0607250:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_2:54022411-54022430 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_2:54022477-54022496 Msa0607250:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_2:54022566-54022585 Msa0607250:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_2:54022663-54022682 Msa0607250:CDS 45.0%
ATGGCTGTGTGGTAATATGC+TGG + chr4_2:54022707-54022726 Msa0607250:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_2:54022791-54022810 Msa0607250:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_2:54022805-54022824 Msa0607250:three_prime_UTR 45.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_2:54020929-54020948 Msa0607250:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_2:54021350-54021369 Msa0607250:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_2:54021624-54021643 Msa0607250:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_2:54021644-54021663 Msa0607250:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_2:54021665-54021684 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_2:54021673-54021692 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_2:54021851-54021870 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_2:54021874-54021893 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_2:54021875-54021894 Msa0607250:CDS 45.0%
CCGTGATTCTCAAATGTGGA+GGG - chr4_2:54021901-54021920 None:intergenic 45.0%
CCCTCCACATTTGAGAATCA+CGG + chr4_2:54021898-54021917 Msa0607250:CDS 45.0%
CCTCCACATTTGAGAATCAC+GGG + chr4_2:54021899-54021918 Msa0607250:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_2:54021952-54021971 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_2:54022116-54022135 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_2:54022196-54022215 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_2:54022262-54022281 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_2:54022389-54022408 Msa0607250:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_2:54022411-54022430 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_2:54022477-54022496 Msa0607250:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_2:54022566-54022585 Msa0607250:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_2:54022663-54022682 Msa0607250:CDS 45.0%
ATGGCTGTGTGGTAATATGC+TGG + chr4_2:54022707-54022726 Msa0607250:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_2:54022791-54022810 Msa0607250:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_2:54022805-54022824 Msa0607250:three_prime_UTR 45.0%
TCCATGATGCCACCTTCATC+TGG + chr4_2:54021410-54021429 Msa0607250:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_2:54021416-54021435 Msa0607250:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_2:54021422-54021441 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_2:54021425-54021444 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_2:54021439-54021458 Msa0607250:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_2:54021446-54021465 None:intergenic 50.0%
CTGCATAGTATCGCCATCAG+GGG - chr4_2:54021588-54021607 None:intergenic 50.0%
GCTGCATAGTATCGCCATCA+GGG - chr4_2:54021589-54021608 None:intergenic 50.0%
! CTGATGGCGATACTATGCAG+CGG + chr4_2:54021588-54021607 Msa0607250:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_2:54021682-54021701 Msa0607250:CDS 50.0%
CCCGTGATTCTCAAATGTGG+AGG - chr4_2:54021902-54021921 None:intergenic 50.0%
ACGCCCGTGATTCTCAAATG+TGG - chr4_2:54021905-54021924 None:intergenic 50.0%
GGGCGTTCATCAGAAGAAAG+AGG + chr4_2:54021919-54021938 Msa0607250:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_2:54021931-54021950 Msa0607250:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_2:54022048-54022067 Msa0607250:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_2:54022065-54022084 None:intergenic 50.0%
GAAGGAGGAAAATGGCTGTG+TGG + chr4_2:54022696-54022715 Msa0607250:CDS 50.0%
TCCATGATGCCACCTTCATC+TGG + chr4_2:54021410-54021429 Msa0607250:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_2:54021416-54021435 Msa0607250:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_2:54021422-54021441 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_2:54021425-54021444 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_2:54021439-54021458 Msa0607250:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_2:54021446-54021465 None:intergenic 50.0%
CTGCATAGTATCGCCATCAG+GGG - chr4_2:54021588-54021607 None:intergenic 50.0%
GCTGCATAGTATCGCCATCA+GGG - chr4_2:54021589-54021608 None:intergenic 50.0%
! CTGATGGCGATACTATGCAG+CGG + chr4_2:54021588-54021607 Msa0607250:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_2:54021682-54021701 Msa0607250:CDS 50.0%
CCCGTGATTCTCAAATGTGG+AGG - chr4_2:54021902-54021921 None:intergenic 50.0%
ACGCCCGTGATTCTCAAATG+TGG - chr4_2:54021905-54021924 None:intergenic 50.0%
GGGCGTTCATCAGAAGAAAG+AGG + chr4_2:54021919-54021938 Msa0607250:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_2:54021931-54021950 Msa0607250:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_2:54022048-54022067 Msa0607250:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_2:54022065-54022084 None:intergenic 50.0%
GAAGGAGGAAAATGGCTGTG+TGG + chr4_2:54022696-54022715 Msa0607250:CDS 50.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_2:54021414-54021433 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_2:54021432-54021451 Msa0607250:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+TGG + chr4_2:54021572-54021591 Msa0607250:CDS 55.0%
CGCTGCATAGTATCGCCATC+AGG - chr4_2:54021590-54021609 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_2:54021837-54021856 Msa0607250:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_2:54021893-54021912 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_2:54021414-54021433 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_2:54021432-54021451 Msa0607250:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+TGG + chr4_2:54021572-54021591 Msa0607250:CDS 55.0%
CGCTGCATAGTATCGCCATC+AGG - chr4_2:54021590-54021609 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_2:54021837-54021856 Msa0607250:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_2:54021893-54021912 None:intergenic 55.0%
GCCATCAGGGGAAGCAAGCA+TGG - chr4_2:54021576-54021595 None:intergenic 60.0%
GCCATCAGGGGAAGCAAGCA+TGG - chr4_2:54021576-54021595 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 54020837 54023042 54020837 ID=Msa0607250;Name=Msa0607250
chr4_2 mRNA 54020837 54023042 54020837 ID=Msa0607250-mRNA-1;Parent=Msa0607250;Name=Msa0607250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=195|0|0.5|1|0|0.5|2|265|475
chr4_2 exon 54020837 54021040 54020837 ID=Msa0607250-mRNA-1:exon:13442;Parent=Msa0607250-mRNA-1
chr4_2 exon 54021359 54023042 54021359 ID=Msa0607250-mRNA-1:exon:13443;Parent=Msa0607250-mRNA-1
chr4_2 five_prime_UTR 54020837 54021031 54020837 ID=Msa0607250-mRNA-1:five_prime_utr;Parent=Msa0607250-mRNA-1
chr4_2 CDS 54021032 54021040 54021032 ID=Msa0607250-mRNA-1:cds;Parent=Msa0607250-mRNA-1
chr4_2 CDS 54021359 54022777 54021359 ID=Msa0607250-mRNA-1:cds;Parent=Msa0607250-mRNA-1
chr4_2 three_prime_UTR 54022778 54023042 54022778 ID=Msa0607250-mRNA-1:three_prime_utr;Parent=Msa0607250-mRNA-1
Gene Sequence

>Msa0607250

ATGATTTTGATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGATGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACGGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGGTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>Msa0607250

MILMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVVICWQDRSLFSISAWRSRK*