AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0647410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647410 MtrunA17_Chr4g0038851 95.975 472 19 0 4 475 1 472 0.0 917
Msa0647410 MtrunA17_Chr5g0396491 74.739 479 113 6 4 475 4 481 0.0 731
Msa0647410 MtrunA17_Chr3g0085681 49.888 445 213 4 30 474 4 438 7.35e-161 462
Msa0647410 MtrunA17_Chr3g0085361 50.986 355 163 5 120 474 9 352 3.34e-126 370
Msa0647410 MtrunA17_Chr7g0224271 33.778 450 216 6 40 472 289 673 4.16e-74 246
Msa0647410 MtrunA17_Chr5g0447691 31.323 431 230 6 48 472 174 544 3.52e-66 222
Msa0647410 MtrunA17_Chr1g0159461 29.837 429 235 8 50 472 224 592 8.20e-56 195
Msa0647410 MtrunA17_Chr3g0125811 29.138 429 238 8 50 472 202 570 4.44e-54 190
Msa0647410 MtrunA17_Chr3g0101141 27.928 444 253 8 35 472 160 542 2.33e-52 185
Msa0647410 MtrunA17_Chr8g0357601 29.955 444 230 12 44 472 175 552 6.94e-51 181
Msa0647410 MtrunA17_Chr4g0031721 28.438 429 238 7 51 471 316 683 8.54e-50 180
Msa0647410 MtrunA17_Chr4g0057891 28.798 441 238 11 45 472 143 520 5.29e-49 175
Msa0647410 MtrunA17_Chr2g0306641 30.455 440 231 15 42 473 116 488 9.28e-48 172
Msa0647410 MtrunA17_Chr4g0031701 26.490 453 264 7 24 467 229 621 4.41e-47 172
Msa0647410 MtrunA17_Chr2g0290241 28.037 428 241 9 52 472 58 425 4.69e-47 168
Msa0647410 MtrunA17_Chr7g0238891 27.103 428 247 5 51 472 372 740 1.66e-46 172
Msa0647410 MtrunA17_Chr4g0031731 26.269 453 265 7 24 467 229 621 3.46e-45 167
Msa0647410 MtrunA17_Chr2g0328391 26.168 428 251 5 51 471 299 668 3.67e-44 164
Msa0647410 MtrunA17_Chr4g0031711 27.174 460 261 11 23 471 336 732 4.35e-44 165
Msa0647410 MtrunA17_Chr2g0328411 26.606 436 234 8 51 471 289 653 5.84e-41 155
Msa0647410 MtrunA17_Chr2g0328431 26.464 427 246 7 51 471 289 653 2.05e-40 154
Msa0647410 MtrunA17_Chr4g0057291 35.349 215 135 1 44 254 285 499 3.22e-38 147
Msa0647410 MtrunA17_Chr4g0057291 31.373 153 98 5 325 471 510 661 2.66e-18 87.8
Msa0647410 MtrunA17_Chr2g0328381 25.172 437 262 8 40 471 264 640 3.81e-38 147
Msa0647410 MtrunA17_Chr2g0294471 29.306 447 235 15 42 472 195 576 6.59e-38 145
Msa0647410 MtrunA17_Chr1g0200401 27.948 458 230 14 45 473 74 460 1.14e-37 143
Msa0647410 MtrunA17_Chr7g0245601 40.314 191 111 2 55 244 442 630 3.17e-37 145
Msa0647410 MtrunA17_Chr7g0245601 36.913 149 89 3 327 472 646 792 5.24e-20 93.6
Msa0647410 MtrunA17_Chr1g0183641 27.689 437 239 12 43 474 115 479 5.57e-37 142
Msa0647410 MtrunA17_Chr2g0294461 27.938 451 239 14 42 472 201 585 9.94e-37 142
Msa0647410 MtrunA17_Chr2g0328401 26.984 378 208 6 51 422 290 605 4.99e-36 141
Msa0647410 MtrunA17_Chr3g0110971 34.685 222 131 4 39 255 207 419 1.47e-34 136
Msa0647410 MtrunA17_Chr3g0110971 36.196 163 91 2 323 472 425 587 4.70e-28 117
Msa0647410 MtrunA17_Chr8g0376381 33.482 224 130 5 39 255 157 368 1.22e-31 127
Msa0647410 MtrunA17_Chr8g0376381 36.601 153 90 3 323 472 374 522 3.35e-28 117
Msa0647410 MtrunA17_Chr3g0114841 25.708 459 250 14 38 471 107 499 7.45e-31 125
Msa0647410 MtrunA17_Chr2g0319091 33.778 225 139 3 48 266 209 429 1.25e-30 125
Msa0647410 MtrunA17_Chr2g0319091 41.611 149 86 1 323 471 431 578 2.46e-29 121
Msa0647410 MtrunA17_Chr5g0445331 31.556 225 145 3 35 251 146 369 7.93e-30 122
Msa0647410 MtrunA17_Chr5g0445331 38.298 141 86 1 332 472 393 532 1.24e-25 109
Msa0647410 MtrunA17_Chr2g0328361 24.541 436 241 10 40 471 263 614 8.86e-30 122
Msa0647410 MtrunA17_Chr2g0328341 28.352 261 171 5 26 278 251 503 1.44e-29 122
Msa0647410 MtrunA17_Chr4g0031741 31.111 225 142 4 48 266 281 498 3.06e-29 121
Msa0647410 MtrunA17_Chr4g0031741 32.000 150 101 1 323 471 500 649 4.18e-16 81.3
Msa0647410 MtrunA17_Chr7g0270031 25.567 485 278 13 4 472 137 554 7.56e-29 119
Msa0647410 MtrunA17_Chr4g0038761 32.124 193 128 2 55 244 88 280 3.93e-26 110
Msa0647410 MtrunA17_Chr4g0038761 39.597 149 84 4 326 472 298 442 8.02e-20 91.7
Msa0647410 MtrunA17_Chr2g0328371 25.000 292 158 7 134 422 13 246 8.50e-26 105
Msa0647410 MtrunA17_Chr1g0207361 63.366 101 35 2 276 375 64 163 2.07e-25 102
Msa0647410 MtrunA17_Chr1g0207361 72.222 54 15 0 85 138 1 54 7.67e-22 92.4
Msa0647410 MtrunA17_Chr4g0053901 24.187 492 293 11 14 471 22 467 2.75e-25 108
Msa0647410 MtrunA17_Chr5g0401421 24.330 485 284 14 22 471 9 445 4.87e-25 107
Msa0647410 MtrunA17_Chr5g0424131 31.169 231 142 4 37 250 107 337 6.60e-25 107
Msa0647410 MtrunA17_Chr5g0424131 26.667 150 107 3 324 471 350 498 3.80e-11 65.1
Msa0647410 MtrunA17_Chr5g0401131 31.651 218 139 4 45 253 108 324 1.69e-24 106
Msa0647410 MtrunA17_Chr4g0076091 31.163 215 140 3 46 253 170 383 2.23e-24 106
Msa0647410 MtrunA17_Chr4g0076091 34.228 149 97 1 323 471 395 542 1.48e-21 97.8
Msa0647410 MtrunA17_Chr2g0329591 31.019 216 137 5 42 247 65 278 5.16e-22 98.6
Msa0647410 MtrunA17_Chr1g0194621 33.728 169 102 5 310 472 354 518 2.40e-21 97.1
Msa0647410 MtrunA17_Chr1g0194621 25.000 248 165 8 42 269 137 383 5.65e-12 67.8
Msa0647410 MtrunA17_Chr4g0039661 28.700 223 149 3 48 263 190 409 6.84e-20 92.4
Msa0647410 MtrunA17_Chr4g0039661 30.055 183 115 6 298 472 378 555 1.75e-13 72.8
Msa0647410 MtrunA17_Chr6g0468561 26.331 357 191 11 127 472 367 662 1.43e-19 92.0
Msa0647410 MtrunA17_Chr4g0012071 25.059 427 242 11 52 472 197 551 1.68e-19 91.3
Msa0647410 MtrunA17_Chr7g0236851 33.333 150 95 3 323 471 511 656 2.94e-19 90.9
Msa0647410 MtrunA17_Chr7g0230731 32.941 170 106 3 22 191 349 510 1.42e-14 76.3
Msa0647410 MtrunA17_Chr7g0230771 32.941 170 106 3 22 191 352 513 1.62e-14 76.3
Msa0647410 MtrunA17_Chr8g0383981 29.126 206 132 8 48 248 136 332 1.42e-13 72.8
Msa0647410 MtrunA17_Chr3g0087711 51.724 58 28 0 202 259 1 58 8.09e-13 63.2
Msa0647410 MtrunA17_Chr2g0323111 26.174 149 108 2 323 471 159 305 2.17e-12 68.2
Msa0647410 MtrunA17_Chr8g0344101 22.414 464 250 14 45 475 217 603 6.48e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0647410 AT1G50420.1 62.603 484 170 5 1 475 1 482 0.0 612
Msa0647410 AT1G66350.1 32.037 437 221 8 39 472 143 506 5.51e-68 226
Msa0647410 AT5G48150.1 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0647410 AT5G48150.4 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0647410 AT5G48150.2 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0647410 AT5G48150.3 30.679 427 231 5 52 472 123 490 5.85e-66 220
Msa0647410 AT2G04890.1 32.938 422 222 5 51 472 53 413 2.62e-65 216
Msa0647410 AT5G17490.1 30.905 453 229 12 34 472 134 516 1.30e-59 204
Msa0647410 AT1G50600.3 29.322 457 251 10 27 472 131 526 1.90e-58 201
Msa0647410 AT1G50600.1 29.386 456 250 10 28 472 203 597 4.25e-58 202
Msa0647410 AT1G50600.2 29.322 457 251 10 27 472 206 601 4.79e-58 202
Msa0647410 AT1G21450.1 30.769 429 232 7 50 472 224 593 6.82e-56 196
Msa0647410 AT1G14920.1 30.112 445 225 12 39 472 160 529 5.52e-53 187
Msa0647410 AT2G01570.1 28.764 445 231 13 39 472 212 581 1.11e-47 173
Msa0647410 AT5G41920.1 30.023 433 227 11 48 474 40 402 9.05e-46 164
Msa0647410 AT1G07530.1 27.955 440 227 10 51 471 396 764 5.73e-44 165
Msa0647410 AT3G54220.1 42.180 211 120 1 36 244 278 488 5.17e-41 155
Msa0647410 AT3G54220.1 35.616 146 92 2 329 474 509 652 7.16e-20 93.2
Msa0647410 AT2G29060.1 26.267 434 250 8 48 471 318 691 6.17e-40 153
Msa0647410 AT2G29060.2 27.189 434 246 10 48 471 281 654 7.30e-40 152
Msa0647410 AT1G07520.2 25.282 443 263 7 40 471 143 528 4.61e-39 149
Msa0647410 AT1G07520.4 25.282 443 263 7 40 471 143 528 4.61e-39 149
Msa0647410 AT5G66770.1 28.899 436 231 12 48 472 217 584 8.05e-39 149
Msa0647410 AT1G07520.3 25.282 443 263 7 40 471 308 693 2.22e-38 149
Msa0647410 AT1G07520.1 25.282 443 263 7 40 471 307 692 2.55e-38 148
Msa0647410 AT5G59450.1 25.806 434 254 7 48 472 224 598 1.56e-37 145
Msa0647410 AT2G29065.1 25.571 438 247 10 51 471 252 627 9.41e-37 143
Msa0647410 AT3G46600.2 25.926 432 254 6 50 475 3 374 2.70e-36 138
Msa0647410 AT2G37650.2 24.826 431 257 6 48 472 332 701 5.39e-36 142
Msa0647410 AT2G37650.1 24.826 431 257 6 48 472 344 713 5.99e-36 141
Msa0647410 AT3G46600.3 25.926 432 254 6 50 475 179 550 6.07e-35 137
Msa0647410 AT3G46600.1 25.843 445 263 7 37 475 199 582 1.57e-34 136
Msa0647410 AT1G63100.2 32.218 239 157 4 43 277 269 506 2.10e-31 128
Msa0647410 AT1G63100.2 33.125 160 94 2 325 471 494 653 1.50e-20 95.5
Msa0647410 AT1G63100.1 32.218 239 157 4 43 277 244 481 2.91e-31 127
Msa0647410 AT1G63100.1 33.125 160 94 2 325 471 469 628 1.03e-20 95.9
Msa0647410 AT3G03450.1 31.839 223 143 5 39 255 171 390 2.63e-30 124
Msa0647410 AT3G03450.1 38.854 157 94 2 317 472 390 545 1.07e-28 119
Msa0647410 AT5G52510.1 26.376 436 246 13 50 472 267 640 3.05e-30 124
Msa0647410 AT1G55580.1 38.255 149 91 1 325 472 297 445 4.39e-28 116
Msa0647410 AT1G55580.1 27.843 255 143 8 47 261 40 293 5.29e-13 71.2
Msa0647410 AT4G37650.1 31.984 247 138 7 24 253 131 364 3.06e-24 106
Msa0647410 AT3G50650.1 26.157 432 235 13 56 472 180 542 5.40e-24 105
Msa0647410 AT4G17230.1 34.000 150 98 1 323 472 377 525 6.54e-23 102
Msa0647410 AT4G17230.1 29.245 212 143 2 46 251 153 363 1.62e-22 100
Msa0647410 AT4G17230.2 34.000 150 98 1 323 472 377 525 6.54e-23 102
Msa0647410 AT4G17230.2 29.245 212 143 2 46 251 153 363 1.62e-22 100
Msa0647410 AT4G08250.1 30.116 259 153 9 6 250 72 316 2.81e-21 97.1
Msa0647410 AT3G49950.1 22.126 461 276 14 29 471 12 407 7.07e-16 79.7
Msa0647410 AT4G00150.1 27.179 195 133 5 283 474 368 556 9.47e-12 67.8

Find 127 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4_3:+53510506 Msa0647410:CDS
CCGAATATGCTTCGGTTTAC+TGG 0.158109 4_3:-53510226 None:intergenic
TTCTTCCTTAGTCATGATTT+TGG 0.181091 4_3:+53509052 Msa0647410:exon
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4_3:+53510652 Msa0647410:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4_3:-53510138 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4_3:+53510468 Msa0647410:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4_3:+53510039 Msa0647410:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4_3:-53509429 None:intergenic
CAGAACAAAGTAACTCATTA+AGG 0.282442 4_3:+53508924 Msa0647410:five_prime_UTR
AGATTTGATTTAGCTGGATT+TGG 0.318965 4_3:+53510628 Msa0647410:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4_3:+53510782 Msa0647410:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4_3:-53510502 None:intergenic
AGAGTGTGAGTTTGAACTTT+TGG 0.331382 4_3:-53508878 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4_3:+53509449 Msa0647410:CDS
CCAGTAAACCGAAGCATATT+CGG 0.339215 4_3:+53510226 Msa0647410:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4_3:+53509796 Msa0647410:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4_3:+53509443 Msa0647410:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4_3:-53510658 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4_3:-53509695 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4_3:+53510507 Msa0647410:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4_3:-53510033 None:intergenic
TTTGGACGAGCACTTGAGTT+TGG 0.364682 4_3:+53510824 Msa0647410:three_prime_UTR
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4_3:+53510249 Msa0647410:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4_3:+53509472 Msa0647410:CDS
GAGTTACTTTGTTCTGTTGT+TGG 0.378375 4_3:-53508917 None:intergenic
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4_3:+53510721 Msa0647410:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4_3:-53510284 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4_3:-53510501 None:intergenic
ACAAGTATGCAATTCCTTGA+TGG 0.395999 4_3:-53509029 None:intergenic
CAAATCCAAGAGGCAAGCTT+TGG 0.398712 4_3:+53508962 Msa0647410:five_prime_UTR
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4_3:-53509923 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4_3:+53510076 Msa0647410:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4_3:-53509904 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4_3:+53509657 Msa0647410:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4_3:-53510066 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4_3:+53509465 Msa0647410:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4_3:-53509703 None:intergenic
TTCAATTTCAAAACCCATCA+AGG 0.420097 4_3:+53509015 Msa0647410:five_prime_UTR
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4_3:-53510313 None:intergenic
CGCTGCATAGTATCGCCATC+AGG 0.429354 4_3:-53509620 None:intergenic
AGTAAGACGATGTGATTGTT+TGG 0.435895 4_3:+53510806 Msa0647410:exon
TTTATGAGATTTGATTTAGC+TGG 0.436460 4_3:+53510622 Msa0647410:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4_3:-53510126 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4_3:+53510526 Msa0647410:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4_3:+53510688 Msa0647410:CDS
CAAGTATGCAATTCCTTGAT+GGG 0.447226 4_3:-53509028 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4_3:+53509493 Msa0647410:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4_3:-53510146 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4_3:+53510403 Msa0647410:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4_3:+53510749 Msa0647410:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4_3:-53510382 None:intergenic
AAATTCCAAAGCTTGCCTCT+TGG 0.452365 4_3:-53508967 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4_3:+53510591 Msa0647410:CDS
TCCATGCTTGCTTCCCCTGA+TGG 0.459836 4_3:+53509605 Msa0647410:CDS
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4_3:-53510137 None:intergenic
GCAAATTGCCGAATATGCTT+CGG 0.476281 4_3:-53510234 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4_3:-53509553 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4_3:-53509529 None:intergenic
ATATGCTTCGGTTTACTGGA+TGG 0.504223 4_3:-53510222 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4_3:-53509782 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4_3:+53509677 Msa0647410:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4_3:-53510194 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4_3:-53509781 None:intergenic
TTACTTTGTTCTGTTGTTGG+TGG 0.520167 4_3:-53508914 None:intergenic
TCAGGCTATTATTGAAGCAA+TGG 0.520201 4_3:+53509814 Msa0647410:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4_3:-53509444 None:intergenic
GCCATCAGGGGAAGCAAGCA+TGG 0.523131 4_3:-53509606 None:intergenic
ACATACCAAAATCATGACTA+AGG 0.527098 4_3:-53509057 None:intergenic
CAAGAACATTATTGCATGTG+AGG 0.529680 4_3:+53510558 Msa0647410:CDS
TCGAATGGAATTCACTTACA+AGG 0.530422 4_3:+53510196 Msa0647410:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4_3:+53510077 Msa0647410:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4_3:+53510599 Msa0647410:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4_3:-53510292 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4_3:+53510573 Msa0647410:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4_3:-53509660 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4_3:+53510477 Msa0647410:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4_3:-53510095 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4_3:+53510294 Msa0647410:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4_3:+53509492 Msa0647410:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4_3:-53509482 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4_3:+53510711 Msa0647410:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4_3:+53509384 Msa0647410:intron
CCAAGCTGATATTGAAAACA+AGG 0.551538 4_3:-53510773 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4_3:-53509476 None:intergenic
AGAAGTGAAATCCACTGAGC+AGG 0.552331 4_3:-53509881 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4_3:+53510740 Msa0647410:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4_3:-53509982 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4_3:+53509932 Msa0647410:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4_3:+53509964 Msa0647410:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4_3:-53509481 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4_3:-53509951 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4_3:-53510441 None:intergenic
GCTATTATTGAAGCAATGGA+AGG 0.565201 4_3:+53509818 Msa0647410:CDS
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4_3:+53510510 Msa0647410:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4_3:+53510559 Msa0647410:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4_3:+53509383 Msa0647410:intron
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4_3:-53509455 None:intergenic
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4_3:+53510372 Msa0647410:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4_3:-53510028 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4_3:+53510696 Msa0647410:CDS
GCTGCATAGTATCGCCATCA+GGG 0.588825 4_3:-53509619 None:intergenic
TACAAAATACTTTCATACAA+AGG 0.591122 4_3:-53510968 None:intergenic
CAACTCTACTCGAGTGTCGA+TGG 0.592320 4_3:+53509715 Msa0647410:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4_3:+53509952 Msa0647410:CDS
ACTTGAGTTTGGCAAAATTG+CGG 0.593940 4_3:+53510835 Msa0647410:three_prime_UTR
TATTATTGAAGCAATGGAAG+GGG 0.594602 4_3:+53509820 Msa0647410:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4_3:+53510671 Msa0647410:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4_3:+53509672 Msa0647410:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4_3:+53510350 Msa0647410:CDS
CATCACTCTTCAAATCCAAG+AGG 0.605474 4_3:+53508952 Msa0647410:five_prime_UTR
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4_3:+53509487 Msa0647410:CDS
CTATTATTGAAGCAATGGAA+GGG 0.615371 4_3:+53509819 Msa0647410:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4_3:+53510366 Msa0647410:CDS
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4_3:-53509931 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4_3:+53510714 Msa0647410:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4_3:+53510578 Msa0647410:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4_3:-53509935 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4_3:-53509932 None:intergenic
TGAGTTTGGCAAAATTGCGG+TGG 0.634786 4_3:+53510838 Msa0647410:three_prime_UTR
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4_3:+53509870 Msa0647410:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4_3:-53509452 None:intergenic
CTGCATAGTATCGCCATCAG+GGG 0.660723 4_3:-53509618 None:intergenic
CAGCGAGATTGACAAAATCA+CGG 0.671324 4_3:-53510865 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4_3:+53510078 Msa0647410:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4_3:+53509494 Msa0647410:CDS
CTGATGGCGATACTATGCAG+CGG 0.726536 4_3:+53509621 Msa0647410:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4_3:+53510081 Msa0647410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAGAAAAAATAAATAA+AGG + chr4_3:53510925-53510944 Msa0647410:three_prime_UTR 10.0%
!! CAATAAGAAAAAATAAATAA+AGG + chr4_3:53510925-53510944 Msa0647410:three_prime_UTR 10.0%
!!! ATTTTTTCTTATTGTCATTA+TGG - chr4_3:53510919-53510938 None:intergenic 15.0%
!!! ATTTTTTCTTATTGTCATTA+TGG - chr4_3:53510919-53510938 None:intergenic 15.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_3:53509362-53509381 Msa0647410:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_3:53510971-53510990 None:intergenic 20.0%
!!! TCATTTCATTTTATGTTACA+TGG + chr4_3:53509362-53509381 Msa0647410:intron 20.0%
!! TACAAAATACTTTCATACAA+AGG - chr4_3:53510971-53510990 None:intergenic 20.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_3:53509122-53509141 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_3:53510477-53510496 Msa0647410:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_3:53510622-53510641 Msa0647410:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_3:53510661-53510680 None:intergenic 25.0%
!! AAAAGTTATGGACTAAAACA+TGG - chr4_3:53509122-53509141 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG + chr4_3:53510477-53510496 Msa0647410:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG + chr4_3:53510622-53510641 Msa0647410:CDS 25.0%
! AACTTACCAAAATAACTTAG+AGG - chr4_3:53510661-53510680 None:intergenic 25.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_3:53508924-53508943 Msa0647410:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_3:53509015-53509034 Msa0647410:five_prime_UTR 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_3:53509052-53509071 Msa0647410:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_3:53509060-53509079 None:intergenic 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_3:53509084-53509103 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_3:53509130-53509149 Msa0647410:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_3:53509271-53509290 Msa0647410:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_3:53509784-53509803 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_3:53509785-53509804 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_3:53509819-53509838 Msa0647410:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_3:53509820-53509839 Msa0647410:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_3:53510164-53510183 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_3:53510181-53510200 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_3:53510181-53510200 Msa0647410:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_3:53510249-53510268 Msa0647410:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_3:53510316-53510335 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_3:53510403-53510422 Msa0647410:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_3:53510417-53510436 Msa0647410:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_3:53510507-53510526 Msa0647410:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_3:53510559-53510578 Msa0647410:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_3:53510628-53510647 Msa0647410:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_3:53510652-53510671 Msa0647410:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_3:53510721-53510740 Msa0647410:CDS 30.0%
CAGAACAAAGTAACTCATTA+AGG + chr4_3:53508924-53508943 Msa0647410:five_prime_UTR 30.0%
TTCAATTTCAAAACCCATCA+AGG + chr4_3:53509015-53509034 Msa0647410:five_prime_UTR 30.0%
! TTCTTCCTTAGTCATGATTT+TGG + chr4_3:53509052-53509071 Msa0647410:exon 30.0%
ACATACCAAAATCATGACTA+AGG - chr4_3:53509060-53509079 None:intergenic 30.0%
ACAACTTACAAGAAGAAAAG+AGG - chr4_3:53509084-53509103 None:intergenic 30.0%
!!! TCCATAACTTTTTCATGAGT+CGG + chr4_3:53509130-53509149 Msa0647410:intron 30.0%
ATGAGTCTACTTGAGAATAT+TGG + chr4_3:53509271-53509290 Msa0647410:intron 30.0%
!! CAAATGCTACTTTCATGAAA+GGG - chr4_3:53509784-53509803 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG - chr4_3:53509785-53509804 None:intergenic 30.0%
CTATTATTGAAGCAATGGAA+GGG + chr4_3:53509819-53509838 Msa0647410:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG + chr4_3:53509820-53509839 Msa0647410:CDS 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG - chr4_3:53510164-53510183 None:intergenic 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG - chr4_3:53510181-53510200 None:intergenic 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG + chr4_3:53510181-53510200 Msa0647410:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG + chr4_3:53510249-53510268 Msa0647410:CDS 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG - chr4_3:53510316-53510335 None:intergenic 30.0%
GAACAAGACTCTAATCATAA+TGG + chr4_3:53510403-53510422 Msa0647410:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG + chr4_3:53510417-53510436 Msa0647410:CDS 30.0%
AAACTCATTCGAGAGATTTA+GGG + chr4_3:53510507-53510526 Msa0647410:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG + chr4_3:53510559-53510578 Msa0647410:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG + chr4_3:53510628-53510647 Msa0647410:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG + chr4_3:53510652-53510671 Msa0647410:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG + chr4_3:53510721-53510740 Msa0647410:CDS 30.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_3:53508881-53508900 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_3:53508917-53508936 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_3:53508920-53508939 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_3:53508974-53508993 Msa0647410:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_3:53509031-53509050 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_3:53509032-53509051 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_3:53509134-53509153 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_3:53509484-53509503 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_3:53509672-53509691 Msa0647410:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_3:53509796-53509815 Msa0647410:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_3:53509814-53509833 Msa0647410:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_3:53509818-53509837 Msa0647410:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_3:53510031-53510050 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_3:53510046-53510065 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_3:53510069-53510088 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_3:53510076-53510095 Msa0647410:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_3:53510077-53510096 Msa0647410:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_3:53510197-53510216 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_3:53510196-53510215 Msa0647410:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_3:53510350-53510369 Msa0647410:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_3:53510366-53510385 Msa0647410:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_3:53510468-53510487 Msa0647410:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_3:53510504-53510523 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_3:53510505-53510524 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_3:53510506-53510525 Msa0647410:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_3:53510558-53510577 Msa0647410:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_3:53510573-53510592 Msa0647410:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_3:53510578-53510597 Msa0647410:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_3:53510711-53510730 Msa0647410:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_3:53510776-53510795 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_3:53510773-53510792 Msa0647410:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_3:53510806-53510825 Msa0647410:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_3:53510835-53510854 Msa0647410:three_prime_UTR 35.0%
! AGAGTGTGAGTTTGAACTTT+TGG - chr4_3:53508881-53508900 None:intergenic 35.0%
TTACTTTGTTCTGTTGTTGG+TGG - chr4_3:53508917-53508936 None:intergenic 35.0%
! GAGTTACTTTGTTCTGTTGT+TGG - chr4_3:53508920-53508939 None:intergenic 35.0%
!!! GCAAGCTTTGGAATTTTCTT+TGG + chr4_3:53508974-53508993 Msa0647410:five_prime_UTR 35.0%
CAAGTATGCAATTCCTTGAT+GGG - chr4_3:53509031-53509050 None:intergenic 35.0%
ACAAGTATGCAATTCCTTGA+TGG - chr4_3:53509032-53509051 None:intergenic 35.0%
! ACCGACTCATGAAAAAGTTA+TGG - chr4_3:53509134-53509153 None:intergenic 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG - chr4_3:53509484-53509503 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG + chr4_3:53509672-53509691 Msa0647410:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG + chr4_3:53509796-53509815 Msa0647410:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG + chr4_3:53509814-53509833 Msa0647410:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG + chr4_3:53509818-53509837 Msa0647410:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG - chr4_3:53510031-53510050 None:intergenic 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG - chr4_3:53510046-53510065 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG - chr4_3:53510069-53510088 None:intergenic 35.0%
GAGAAACTTCGTGTTAAAAC+CGG + chr4_3:53510076-53510095 Msa0647410:CDS 35.0%
AGAAACTTCGTGTTAAAACC+GGG + chr4_3:53510077-53510096 Msa0647410:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG - chr4_3:53510197-53510216 None:intergenic 35.0%
TCGAATGGAATTCACTTACA+AGG + chr4_3:53510196-53510215 Msa0647410:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG + chr4_3:53510350-53510369 Msa0647410:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG + chr4_3:53510366-53510385 Msa0647410:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG + chr4_3:53510468-53510487 Msa0647410:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG - chr4_3:53510504-53510523 None:intergenic 35.0%
AATCTCTCGAATGAGTTTCT+TGG - chr4_3:53510505-53510524 None:intergenic 35.0%
GAAACTCATTCGAGAGATTT+AGG + chr4_3:53510506-53510525 Msa0647410:CDS 35.0%
CAAGAACATTATTGCATGTG+AGG + chr4_3:53510558-53510577 Msa0647410:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG + chr4_3:53510573-53510592 Msa0647410:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG + chr4_3:53510578-53510597 Msa0647410:CDS 35.0%
TTGTGAGGCATATAGAATGA+AGG + chr4_3:53510711-53510730 Msa0647410:CDS 35.0%
CCAAGCTGATATTGAAAACA+AGG - chr4_3:53510776-53510795 None:intergenic 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG + chr4_3:53510773-53510792 Msa0647410:CDS 35.0%
AGTAAGACGATGTGATTGTT+TGG + chr4_3:53510806-53510825 Msa0647410:exon 35.0%
ACTTGAGTTTGGCAAAATTG+CGG + chr4_3:53510835-53510854 Msa0647410:three_prime_UTR 35.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_3:53508952-53508971 Msa0647410:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_3:53508970-53508989 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_3:53509241-53509260 Msa0647410:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_3:53509384-53509403 Msa0647410:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_3:53509432-53509451 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_3:53509485-53509504 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_3:53509487-53509506 Msa0647410:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_3:53509492-53509511 Msa0647410:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_3:53509493-53509512 Msa0647410:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_3:53509494-53509513 Msa0647410:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_3:53509532-53509551 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_3:53509556-53509575 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_3:53509663-53509682 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_3:53510036-53510055 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_3:53510039-53510058 Msa0647410:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_3:53510078-53510097 Msa0647410:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_3:53510129-53510148 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_3:53510128-53510147 Msa0647410:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_3:53510129-53510148 Msa0647410:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_3:53510140-53510159 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_3:53510141-53510160 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_3:53510225-53510244 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_3:53510226-53510245 Msa0647410:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_3:53510237-53510256 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_3:53510287-53510306 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_3:53510294-53510313 Msa0647410:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_3:53510372-53510391 Msa0647410:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_3:53510385-53510404 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_3:53510526-53510545 Msa0647410:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_3:53510531-53510550 Msa0647410:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_3:53510591-53510610 Msa0647410:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_3:53510671-53510690 Msa0647410:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_3:53510688-53510707 Msa0647410:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_3:53510714-53510733 Msa0647410:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_3:53510749-53510768 Msa0647410:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_3:53510782-53510801 Msa0647410:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_3:53510868-53510887 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_3:53510900-53510919 None:intergenic 40.0%
CATCACTCTTCAAATCCAAG+AGG + chr4_3:53508952-53508971 Msa0647410:five_prime_UTR 40.0%
AAATTCCAAAGCTTGCCTCT+TGG - chr4_3:53508970-53508989 None:intergenic 40.0%
TTCTGCTTTGCTTTGCTTGT+TGG + chr4_3:53509241-53509260 Msa0647410:intron 40.0%
GTACAGCTATGTTGCAAGAA+GGG + chr4_3:53509384-53509403 Msa0647410:intron 40.0%
CATCATGGAGAAGAGCTTTA+AGG - chr4_3:53509432-53509451 None:intergenic 40.0%
GATTTCAGTTCCTTAAGCCA+AGG - chr4_3:53509485-53509504 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG + chr4_3:53509487-53509506 Msa0647410:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG + chr4_3:53509492-53509511 Msa0647410:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG + chr4_3:53509493-53509512 Msa0647410:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG + chr4_3:53509494-53509513 Msa0647410:CDS 40.0%
TTGCACAAGTGAGCAACAAA+TGG - chr4_3:53509532-53509551 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG - chr4_3:53509556-53509575 None:intergenic 40.0%
TATCCTATCAGCAAGTGCTT+CGG - chr4_3:53509663-53509682 None:intergenic 40.0%
TGCTGACAACAGGATTGAAT+TGG - chr4_3:53510036-53510055 None:intergenic 40.0%
CAATCCTGTTGTCAGCAAAT+TGG + chr4_3:53510039-53510058 Msa0647410:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG + chr4_3:53510078-53510097 Msa0647410:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG - chr4_3:53510129-53510148 None:intergenic 40.0%
! AATTGCATACCCTTTTAGCC+TGG + chr4_3:53510128-53510147 Msa0647410:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG + chr4_3:53510129-53510148 Msa0647410:CDS 40.0%
TTCATCATCCCAGGCTAAAA+GGG - chr4_3:53510140-53510159 None:intergenic 40.0%
TTTCATCATCCCAGGCTAAA+AGG - chr4_3:53510141-53510160 None:intergenic 40.0%
ATATGCTTCGGTTTACTGGA+TGG - chr4_3:53510225-53510244 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG + chr4_3:53510226-53510245 Msa0647410:CDS 40.0%
GCAAATTGCCGAATATGCTT+CGG - chr4_3:53510237-53510256 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG - chr4_3:53510287-53510306 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG + chr4_3:53510294-53510313 Msa0647410:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG + chr4_3:53510372-53510391 Msa0647410:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG - chr4_3:53510385-53510404 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG + chr4_3:53510526-53510545 Msa0647410:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG + chr4_3:53510531-53510550 Msa0647410:CDS 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG + chr4_3:53510591-53510610 Msa0647410:CDS 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG + chr4_3:53510671-53510690 Msa0647410:CDS 40.0%
AAGAGGTTCATGCAGAGTTA+TGG + chr4_3:53510688-53510707 Msa0647410:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG + chr4_3:53510714-53510733 Msa0647410:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG + chr4_3:53510749-53510768 Msa0647410:CDS 40.0%
CAATATCAGCTTGGAGATCT+AGG + chr4_3:53510782-53510801 Msa0647410:CDS 40.0%
CAGCGAGATTGACAAAATCA+CGG - chr4_3:53510868-53510887 None:intergenic 40.0%
! ATGGCTTATGATTTTGCGCA+TGG - chr4_3:53510900-53510919 None:intergenic 40.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_3:53508962-53508981 Msa0647410:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_3:53509383-53509402 Msa0647410:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_3:53509657-53509676 Msa0647410:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_3:53509677-53509696 Msa0647410:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_3:53509698-53509717 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_3:53509706-53509725 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_3:53509884-53509903 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_3:53509907-53509926 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_3:53509908-53509927 Msa0647410:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_3:53509934-53509953 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_3:53509932-53509951 Msa0647410:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_3:53509954-53509973 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_3:53509985-53510004 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_3:53510149-53510168 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_3:53510229-53510248 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_3:53510295-53510314 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_3:53510422-53510441 Msa0647410:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_3:53510444-53510463 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_3:53510510-53510529 Msa0647410:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_3:53510599-53510618 Msa0647410:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_3:53510696-53510715 Msa0647410:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_3:53510740-53510759 Msa0647410:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_3:53510824-53510843 Msa0647410:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_3:53510838-53510857 Msa0647410:three_prime_UTR 45.0%
CAAATCCAAGAGGCAAGCTT+TGG + chr4_3:53508962-53508981 Msa0647410:five_prime_UTR 45.0%
GGTACAGCTATGTTGCAAGA+AGG + chr4_3:53509383-53509402 Msa0647410:intron 45.0%
! TTACCGAAGCACTTGCTGAT+AGG + chr4_3:53509657-53509676 Msa0647410:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG + chr4_3:53509677-53509696 Msa0647410:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG - chr4_3:53509698-53509717 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG - chr4_3:53509706-53509725 None:intergenic 45.0%
AGAAGTGAAATCCACTGAGC+AGG - chr4_3:53509884-53509903 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG - chr4_3:53509907-53509926 None:intergenic 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG + chr4_3:53509908-53509927 Msa0647410:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG - chr4_3:53509934-53509953 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG + chr4_3:53509932-53509951 Msa0647410:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG - chr4_3:53509954-53509973 None:intergenic 45.0%
CAATCAGTCTATGAGCTACC+TGG - chr4_3:53509985-53510004 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG - chr4_3:53510149-53510168 None:intergenic 45.0%
CCGAATATGCTTCGGTTTAC+TGG - chr4_3:53510229-53510248 None:intergenic 45.0%
! GGTGATGATGAACTTGAGTC+AGG - chr4_3:53510295-53510314 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG + chr4_3:53510422-53510441 Msa0647410:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG - chr4_3:53510444-53510463 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG + chr4_3:53510510-53510529 Msa0647410:CDS 45.0%
GGCATGAAAAGCTGGATAAG+TGG + chr4_3:53510599-53510618 Msa0647410:CDS 45.0%
CATGCAGAGTTATGGTTGTG+AGG + chr4_3:53510696-53510715 Msa0647410:CDS 45.0%
ATGGCTGTGTGCTAATATGC+TGG + chr4_3:53510740-53510759 Msa0647410:CDS 45.0%
TTTGGACGAGCACTTGAGTT+TGG + chr4_3:53510824-53510843 Msa0647410:three_prime_UTR 45.0%
TGAGTTTGGCAAAATTGCGG+TGG + chr4_3:53510838-53510857 Msa0647410:three_prime_UTR 45.0%
TCCATGATGCCACCTTCATC+TGG + chr4_3:53509443-53509462 Msa0647410:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_3:53509449-53509468 Msa0647410:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_3:53509455-53509474 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_3:53509458-53509477 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_3:53509472-53509491 Msa0647410:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_3:53509479-53509498 None:intergenic 50.0%
CTGCATAGTATCGCCATCAG+GGG - chr4_3:53509621-53509640 None:intergenic 50.0%
GCTGCATAGTATCGCCATCA+GGG - chr4_3:53509622-53509641 None:intergenic 50.0%
! CTGATGGCGATACTATGCAG+CGG + chr4_3:53509621-53509640 Msa0647410:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_3:53509715-53509734 Msa0647410:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_3:53509935-53509954 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_3:53509938-53509957 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_3:53509952-53509971 Msa0647410:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_3:53509964-53509983 Msa0647410:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_3:53510081-53510100 Msa0647410:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_3:53510098-53510117 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG + chr4_3:53509443-53509462 Msa0647410:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG + chr4_3:53509449-53509468 Msa0647410:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG - chr4_3:53509455-53509474 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG - chr4_3:53509458-53509477 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG + chr4_3:53509472-53509491 Msa0647410:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG - chr4_3:53509479-53509498 None:intergenic 50.0%
CTGCATAGTATCGCCATCAG+GGG - chr4_3:53509621-53509640 None:intergenic 50.0%
GCTGCATAGTATCGCCATCA+GGG - chr4_3:53509622-53509641 None:intergenic 50.0%
! CTGATGGCGATACTATGCAG+CGG + chr4_3:53509621-53509640 Msa0647410:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG + chr4_3:53509715-53509734 Msa0647410:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG - chr4_3:53509935-53509954 None:intergenic 50.0%
ACGCCGGTGATTCTCAAATG+TGG - chr4_3:53509938-53509957 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG + chr4_3:53509952-53509971 Msa0647410:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG + chr4_3:53509964-53509983 Msa0647410:CDS 50.0%
ACTTCGTGTTAAAACCGGGG+AGG + chr4_3:53510081-53510100 Msa0647410:CDS 50.0%
ACTAATTGCTAGTGCCTCCC+CGG - chr4_3:53510098-53510117 None:intergenic 50.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_3:53509447-53509466 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_3:53509465-53509484 Msa0647410:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+TGG + chr4_3:53509605-53509624 Msa0647410:CDS 55.0%
CGCTGCATAGTATCGCCATC+AGG - chr4_3:53509623-53509642 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_3:53509870-53509889 Msa0647410:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_3:53509926-53509945 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG - chr4_3:53509447-53509466 None:intergenic 55.0%
! GCTTTGGATCTCCACACCCT+TGG + chr4_3:53509465-53509484 Msa0647410:CDS 55.0%
TCCATGCTTGCTTCCCCTGA+TGG + chr4_3:53509605-53509624 Msa0647410:CDS 55.0%
CGCTGCATAGTATCGCCATC+AGG - chr4_3:53509623-53509642 None:intergenic 55.0%
ATGCTGCTGAACCTGCTCAG+TGG + chr4_3:53509870-53509889 Msa0647410:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG - chr4_3:53509926-53509945 None:intergenic 55.0%
GCCATCAGGGGAAGCAAGCA+TGG - chr4_3:53509609-53509628 None:intergenic 60.0%
GCCATCAGGGGAAGCAAGCA+TGG - chr4_3:53509609-53509628 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 53508870 53511075 53508870 ID=Msa0647410;Name=Msa0647410
chr4_3 mRNA 53508870 53511075 53508870 ID=Msa0647410-mRNA-1;Parent=Msa0647410;Name=Msa0647410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=195|0|0.5|1|0|0.5|2|265|475
chr4_3 exon 53508870 53509073 53508870 ID=Msa0647410-mRNA-1:exon:13370;Parent=Msa0647410-mRNA-1
chr4_3 exon 53509392 53511075 53509392 ID=Msa0647410-mRNA-1:exon:13371;Parent=Msa0647410-mRNA-1
chr4_3 five_prime_UTR 53508870 53509064 53508870 ID=Msa0647410-mRNA-1:five_prime_utr;Parent=Msa0647410-mRNA-1
chr4_3 CDS 53509065 53509073 53509065 ID=Msa0647410-mRNA-1:cds;Parent=Msa0647410-mRNA-1
chr4_3 CDS 53509392 53510810 53509392 ID=Msa0647410-mRNA-1:cds;Parent=Msa0647410-mRNA-1
chr4_3 three_prime_UTR 53510811 53511075 53510811 ID=Msa0647410-mRNA-1:three_prime_utr;Parent=Msa0647410-mRNA-1
Gene Sequence

>Msa0647410

ATGATTTTGATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGATGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>Msa0647410

MILMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK*