AlfalfaGEDB | Msa0613350

M. sativa cultivar zhongmu-4 / Msa0613350

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0613350	XP_003608268.1	80.716	363	39	6	1	337	1	358	0.0	549

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0613350	sp\|Q9LDR8\|MY102_ARATH	53.719	363	128	11	1	337	1	349	9.05e-111	329

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0613350	G7JSV1	80.716	363	39	6	1	337	1	358	0.0	549

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TFs/TRs:

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Gene ID	Type	Classification
Msa0613350	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0613350	Msa0653900	0.872948	2.147068e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0613350	MtrunA17_Chr4g0048511	80.716	363	39	6	1	337	1	358	0.0	549
Msa0613350	MtrunA17_Chr2g0281071	54.213	356	133	11	1	337	1	345	1.04e-100	300
Msa0613350	MtrunA17_Chr2g0323411	47.383	363	128	14	1	337	1	326	5.72e-89	270
Msa0613350	MtrunA17_Chr4g0039971	55.981	209	82	4	1	208	2	201	1.25e-76	239
Msa0613350	MtrunA17_Chr3g0115821	74.627	134	33	1	1	134	1	133	2.08e-76	238
Msa0613350	MtrunA17_Chr7g0217741	74.627	134	33	1	1	134	1	133	4.44e-75	233
Msa0613350	MtrunA17_Chr6g0453471	69.737	152	45	1	1	152	1	151	5.47e-75	235
Msa0613350	MtrunA17_Chr4g0042671	75.758	132	31	1	1	132	1	131	4.32e-74	234
Msa0613350	MtrunA17_Chr5g0410961	69.281	153	43	2	1	150	1	152	7.63e-73	229
Msa0613350	MtrunA17_Chr5g0395281	54.146	205	73	5	1	186	1	203	1.81e-68	216

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0613350	AT4G21440.1	53.719	363	128	11	1	337	1	349	9.20e-112	329
Msa0613350	AT4G05100.1	50.139	359	119	8	1	337	1	321	4.05e-97	291
Msa0613350	AT5G54230.1	58.621	232	87	6	13	239	12	239	1.67e-82	253
Msa0613350	AT4G28110.1	83.846	130	20	1	1	130	1	129	1.52e-81	249
Msa0613350	AT5G16770.4	73.469	147	38	1	1	147	1	146	1.02e-78	244
Msa0613350	AT5G16770.3	73.469	147	38	1	1	147	1	146	1.02e-78	244
Msa0613350	AT5G16770.1	73.469	147	38	1	1	147	1	146	2.18e-78	243
Msa0613350	AT5G16770.2	73.469	147	38	1	1	147	1	146	2.18e-78	243
Msa0613350	AT3G02940.2	77.206	136	30	1	1	136	1	135	8.51e-77	238
Msa0613350	AT3G02940.1	77.206	136	30	1	1	136	1	135	2.25e-76	238

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Find 61 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTATAGCACATACTACTA+TGG	0.303884	4_2:+62180952	Msa0613350:exon
AAGAATAATGGCCTCAAAAG+AGG	0.510219	4_2:+62180995	Msa0613350:CDS
AAGAGGACCATGGACGCAAG+AGG	0.629926	4_2:+62181012	Msa0613350:CDS
AAGATAGGTCCAAAAGATCA+AGG	0.542013	4_2:-62181675	None:intergenic
AATCGTCCTCTCTTTATATC+CGG	0.517026	4_2:-62181284	None:intergenic
AATGCAAATCATTTAATTGT+TGG	0.407951	4_2:-62182005	None:intergenic
AATTGTTGGAAGCCAGATTC+AGG	0.314520	4_2:-62181991	None:intergenic
ACAATGAAATCAAGAATTAT+TGG	0.309694	4_2:+62181590	Msa0613350:CDS
ACACCTAAGCTATATGAGAT+AGG	0.463774	4_2:-62182261	None:intergenic
ACTATAGCACATACTACTAT+GGG	0.559030	4_2:+62180953	Msa0613350:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATATGATATGTCTGAAAAG+AGG	-	chr4_2:62180855-62180874	None:intergenic	30.0%
AACTATAGCACATACTACTA+TGG	+	chr4_2:62180952-62180971	Msa0613350:exon	30.0%
ACTATAGCACATACTACTAT+GGG	+	chr4_2:62180953-62180972	Msa0613350:exon	30.0%
ATTATTGGAACACTCACATT+AGG	+	chr4_2:62181605-62181624	Msa0613350:CDS	30.0%
TAGGAAAAGACTGTTAAGAA+TGG	+	chr4_2:62181624-62181643	Msa0613350:CDS	30.0%
AGGAAAAGACTGTTAAGAAT+GGG	+	chr4_2:62181625-62181644	Msa0613350:CDS	30.0%
TATTCGTCAAATTCGAATTC+TGG	-	chr4_2:62182179-62182198	None:intergenic	30.0%
TCGAATTTGACGAATATCAA+TGG	+	chr4_2:62182183-62182202	Msa0613350:CDS	30.0%
TTTCCTATCTCATATAGCTT+AGG	+	chr4_2:62182258-62182277	Msa0613350:CDS	30.0%
GATATGATATGTCTGAAAAG+AGG	-	chr4_2:62180855-62180874	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	62180842	62182299	62180842	ID=Msa0613350;Name=Msa0613350
chr4_2	mRNA	62180842	62182299	62180842	ID=Msa0613350-mRNA-1;Parent=Msa0613350;Name=Msa0613350-mRNA-1;_AED=0.07;_eAED=0.07;_QI=129\|1\|0.66\|1\|1\|1\|3\|0\|337
chr4_2	exon	62180842	62181106	62180842	ID=Msa0613350-mRNA-1:exon:17011;Parent=Msa0613350-mRNA-1
chr4_2	exon	62181231	62181360	62181231	ID=Msa0613350-mRNA-1:exon:17012;Parent=Msa0613350-mRNA-1
chr4_2	exon	62181552	62182299	62181552	ID=Msa0613350-mRNA-1:exon:17013;Parent=Msa0613350-mRNA-1
chr4_2	five_prime_UTR	62180842	62180970	62180842	ID=Msa0613350-mRNA-1:five_prime_utr;Parent=Msa0613350-mRNA-1
chr4_2	CDS	62180971	62181106	62180971	ID=Msa0613350-mRNA-1:cds;Parent=Msa0613350-mRNA-1
chr4_2	CDS	62181231	62181360	62181231	ID=Msa0613350-mRNA-1:cds;Parent=Msa0613350-mRNA-1
chr4_2	CDS	62181552	62182299	62181552	ID=Msa0613350-mRNA-1:cds;Parent=Msa0613350-mRNA-1

Gene Sequence

>Msa0613350

ATGGGAAGAGCACCTTGTTGTGAAAAGAATAATGGCCTCAAAAGAGGACCATGGACGCAAGAGGAAGACCAAAAACTTATAGCTTATATTCAAAAACATGGTTATGGCAACTGGAGATTACTCCCAAAGAATGCTGGATTACAAAGATGTGGAAAGAGTTGTCGTCTCCGTTGGACAAACTATCTCCGACCGGATATAAAGAGAGGACGATTCTCTTTTGAAGAAGAAGAAACAATAATTCATCTGCATAGCATTCTTGGCAACAAGTGGTCTTCAATTGCTTCTAGGTTACCAGGAAGAACAGACAATGAAATCAAGAATTATTGGAACACTCACATTAGGAAAAGACTGTTAAGAATGGGAATTGATCCTGTGACACATAATCCACGCCTTGATCTTTTGGACCTATCTTCTATCATAAATTCATCTCTCTATGCCTCTACCTCATCACAAATGAACATCCAAAGGCTAATTGGTACACAATCAATAGTGAACACAGAGATTCTAAAGTTGGCTTCATCACTCTTCTCTTCTCAAAATGTACAAGAGAATTATCAAATTGATCAAAACCAACAAATTAGCTCACACTTGGATCAAGAAGCTTGCAACATGTTGTTGAATCCACCTTGTGGTCATAATAATTCAATGCCTTTCACTCAAACACACTCAGTTGAATCCAATGAGAATCTATACTCGTCATTTTTACCTGAATCTGGCTTCCAACAATTAAATGATTTGCATTGCAATGGAATTGCTTCAAGTAATGTAACAGAGGATTTTGTTCATCAATTACCATGCTATAACTACTCAAGTTCTGATTATCATGCAAATGATTTAATTATGGAGCCTCACATATCAGAAACTTCAACCTTTCATTGCAATAACAACAACCAGAATTCGAATTTGACGAATATCAATGGAAGCAGTAATGAAGATGAGACAGAGAGCTATGTTAGCAGCAACATATTTGAATTTCCTATCTCATATAGCTTAGGTGTGAATGAGTTCATGTAA

Protein sequence

>Msa0613350

MGRAPCCEKNNGLKRGPWTQEEDQKLIAYIQKHGYGNWRLLPKNAGLQRCGKSCRLRWTNYLRPDIKRGRFSFEEEETIIHLHSILGNKWSSIASRLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHNPRLDLLDLSSIINSSLYASTSSQMNIQRLIGTQSIVNTEILKLASSLFSSQNVQENYQIDQNQQISSHLDQEACNMLLNPPCGHNNSMPFTQTHSVESNENLYSSFLPESGFQQLNDLHCNGIASSNVTEDFVHQLPCYNYSSSDYHANDLIMEPHISETSTFHCNNNNQNSNLTNINGSSNEDETESYVSSNIFEFPISYSLGVNEFM*