AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614100 MtrunA17_Chr4g0049901 90.850 153 12 2 1 152 88 239 1.53e-90 263
Msa0614100 MtrunA17_Chr5g0412461 41.818 165 80 3 1 150 62 225 4.35e-30 108
Msa0614100 MtrunA17_Chr5g0412271 42.771 166 78 3 1 150 62 226 4.37e-29 106
Msa0614100 MtrunA17_Chr5g0427401 34.545 165 90 4 1 150 62 223 2.27e-16 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614100 AT2G22540.2 36.517 178 84 4 1 150 57 233 2.90e-22 89.0
Msa0614100 AT2G22540.3 36.517 178 84 4 1 150 62 238 3.39e-22 89.0
Msa0614100 AT2G22540.1 36.517 178 84 4 1 150 62 238 3.39e-22 89.0
Msa0614100 AT4G24540.1 39.623 159 83 5 1 149 62 217 1.93e-20 84.0

Find 47 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGGAACAAACCTTTATTT+TGG 0.154101 4_2:+63006288 None:intergenic
AATCATTGGAATCTGTTATT+AGG 0.154695 4_2:-63005120 Msa0614100:CDS
TAAGAAGCACCTCTAGTTTC+TGG 0.187200 4_2:+63006332 None:intergenic
TATACGATATGGTATGTTTA+TGG 0.191406 4_2:-63004568 Msa0614100:three_prime_UTR
TATCATTTACCTTTCTCTTA+AGG 0.249055 4_2:+63006116 None:intergenic
GACAGTATTGCTTCCTTTCT+AGG 0.249592 4_2:-63004769 Msa0614100:three_prime_UTR
TTCAGCAAATCACAGATTAT+TGG 0.255559 4_2:-63006759 Msa0614100:CDS
ATCATTGGAATCTGTTATTA+GGG 0.269501 4_2:-63005119 Msa0614100:CDS
ATCATTTACCTTTCTCTTAA+GGG 0.271180 4_2:+63006117 None:intergenic
GTCCCCATCGTCCATTTATA+AGG 0.311501 4_2:+63005143 None:intergenic
GTCTAGTGTTTCCAAAATAA+AGG 0.316806 4_2:-63006299 Msa0614100:intron
AGTGACACTTCTCTCAAGTT+GGG 0.338517 4_2:-63005064 Msa0614100:CDS
CCAAGTTGATGTATACGATA+TGG 0.348378 4_2:-63004579 Msa0614100:three_prime_UTR
TAGGTACCAGACCTTATAAA+TGG 0.357027 4_2:-63005154 Msa0614100:CDS
CTCTCCTTCTCACAGTCCTC+TGG 0.374200 4_2:+63004792 None:intergenic
TCACTGCCATCGTCGTCTTC+AGG 0.376609 4_2:+63005082 None:intergenic
TCCATCTACTGATCAGCTTC+AGG 0.394954 4_2:-63006732 Msa0614100:intron
GTTAAGGAAAATGAGGCCTT+TGG 0.396182 4_2:-63004682 Msa0614100:three_prime_UTR
TTACCACATGTTTCAATCTT+CGG 0.404641 4_2:+63005275 None:intergenic
TGGACGATGGGGACAATCAT+TGG 0.410576 4_2:-63005134 Msa0614100:CDS
CCATATCGTATACATCAACT+TGG 0.431123 4_2:+63004579 None:intergenic
TAAATGTAAAGTAATAGATA+TGG 0.441708 4_2:-63004704 Msa0614100:three_prime_UTR
TAAAGTAATAGATATGGTTA+AGG 0.446785 4_2:-63004698 Msa0614100:three_prime_UTR
CAAGTAATTGAAAGGCGTAA+TGG 0.461408 4_2:-63006785 Msa0614100:CDS
CAGTGACACTTCTCTCAAGT+TGG 0.471667 4_2:-63005065 Msa0614100:CDS
CATCGTCCATTTATAAGGTC+TGG 0.478047 4_2:+63005148 None:intergenic
CTTCAACACAGGCAATTGAA+TGG 0.479094 4_2:-63006388 Msa0614100:intron
GGTTGATCATCGACCTAGAA+AGG 0.492292 4_2:+63004756 None:intergenic
ACCTGAAGCTGATCAGTAGA+TGG 0.520990 4_2:+63006731 None:intergenic
CATCGACACCCTTAAGAGAA+AGG 0.526872 4_2:-63006125 Msa0614100:intron
CAGAGGACTGTGAGAAGGAG+AGG 0.553386 4_2:-63004791 Msa0614100:three_prime_UTR
AGATATGGTTAAGGAAAATG+AGG 0.554813 4_2:-63004689 Msa0614100:three_prime_UTR
AGACCTTATAAATGGACGAT+GGG 0.561354 4_2:-63005146 Msa0614100:CDS
ATAAATAAAATTTCATCCAG+AGG 0.569103 4_2:-63004808 Msa0614100:exon
AACTACAGTTATACAACCAA+AGG 0.587592 4_2:+63004666 None:intergenic
GTATGGAACAAGTAATTGAA+AGG 0.588861 4_2:-63006793 Msa0614100:CDS
GTGACACTTCTCTCAAGTTG+GGG 0.597855 4_2:-63005063 Msa0614100:intron
TAGTTATACAGTAATATACA+TGG 0.602066 4_2:-63006850 Msa0614100:five_prime_UTR
TCATCCAGAGGACTGTGAGA+AGG 0.602265 4_2:-63004796 Msa0614100:three_prime_UTR
GAAGCTGATCAGTAGATGGA+TGG 0.615480 4_2:+63006735 None:intergenic
ATATATGTAAACACTTTCTG+TGG 0.627647 4_2:+63004604 None:intergenic
CATATCGTATACATCAACTT+GGG 0.647453 4_2:+63004580 None:intergenic
CAGACCTTATAAATGGACGA+TGG 0.661139 4_2:-63005147 Msa0614100:CDS
AAGATAAATCTCGTGAACTG+AGG 0.669525 4_2:-63006497 Msa0614100:intron
AAACCGAAGATTGAAACATG+TGG 0.671175 4_2:-63005278 Msa0614100:intron
AGAAGAACTCCAGAAACTAG+AGG 0.675750 4_2:-63006341 Msa0614100:CDS
GACCTTATAAATGGACGATG+GGG 0.689950 4_2:-63005145 Msa0614100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATGTTAAATATAAAATAA+AGG - chr4_2:63005765-63005784 Msa0614100:intron 10.0%
!!! TACATTTTTTTTTTTTATAC+TGG + chr4_2:63006666-63006685 None:intergenic 10.0%
!! CTATGTTAAATATAAAATAA+AGG - chr4_2:63005765-63005784 Msa0614100:intron 10.0%
!!! TACATTTTTTTTTTTTATAC+TGG + chr4_2:63006666-63006685 None:intergenic 10.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_2:63005331-63005350 Msa0614100:intron 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_2:63005398-63005417 Msa0614100:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_2:63005822-63005841 Msa0614100:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_2:63005823-63005842 Msa0614100:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_2:63005911-63005930 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_2:63005912-63005931 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_2:63006656-63006675 None:intergenic 15.0%
!! TAAATGTAAAGTAATAGATA+TGG - chr4_2:63006684-63006703 Msa0614100:intron 15.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_2:63005331-63005350 Msa0614100:intron 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_2:63005398-63005417 Msa0614100:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_2:63005822-63005841 Msa0614100:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_2:63005823-63005842 Msa0614100:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_2:63005911-63005930 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_2:63005912-63005931 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_2:63006656-63006675 None:intergenic 15.0%
!! TAAATGTAAAGTAATAGATA+TGG - chr4_2:63006684-63006703 Msa0614100:intron 15.0%
!! TAGTTATACAGTAATATACA+TGG - chr4_2:63004538-63004557 Msa0614100:three_prime_UTR 20.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_2:63005307-63005326 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_2:63005316-63005335 Msa0614100:intron 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_2:63005989-63006008 Msa0614100:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_2:63006162-63006181 Msa0614100:intron 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_2:63006163-63006182 Msa0614100:intron 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_2:63006416-63006435 Msa0614100:intron 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_2:63006580-63006599 Msa0614100:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_2:63006690-63006709 Msa0614100:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_2:63006752-63006771 Msa0614100:CDS 20.0%
!! TAGTTATACAGTAATATACA+TGG - chr4_2:63004538-63004557 Msa0614100:three_prime_UTR 20.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_2:63005307-63005326 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_2:63005316-63005335 Msa0614100:intron 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_2:63005989-63006008 Msa0614100:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_2:63006162-63006181 Msa0614100:intron 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_2:63006163-63006182 Msa0614100:intron 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_2:63006416-63006435 Msa0614100:intron 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_2:63006580-63006599 Msa0614100:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_2:63006690-63006709 Msa0614100:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_2:63006752-63006771 Msa0614100:CDS 20.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_2:63004735-63004754 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_2:63004736-63004755 None:intergenic 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_2:63004811-63004830 Msa0614100:exon 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_2:63004826-63004845 Msa0614100:exon 25.0%
! GTATATATATGCACCTAATT+AGG + chr4_2:63004938-63004957 None:intergenic 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_2:63005155-63005174 Msa0614100:intron 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_2:63005221-63005240 Msa0614100:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_2:63005274-63005293 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_2:63005275-63005294 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_2:63005300-63005319 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_2:63005336-63005355 Msa0614100:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_2:63005565-63005584 None:intergenic 25.0%
! TTTGATTATATTTACCACAC+AGG - chr4_2:63006068-63006087 Msa0614100:intron 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_2:63006174-63006193 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_2:63006215-63006234 Msa0614100:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_2:63006268-63006287 Msa0614100:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_2:63006269-63006288 Msa0614100:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_2:63006363-63006382 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_2:63006375-63006394 Msa0614100:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_2:63006415-63006434 Msa0614100:intron 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_2:63006787-63006806 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_2:63006820-63006839 Msa0614100:five_prime_UTR 25.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_2:63004735-63004754 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_2:63004736-63004755 None:intergenic 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_2:63004811-63004830 Msa0614100:exon 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_2:63004826-63004845 Msa0614100:exon 25.0%
! GTATATATATGCACCTAATT+AGG + chr4_2:63004938-63004957 None:intergenic 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_2:63005155-63005174 Msa0614100:intron 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_2:63005221-63005240 Msa0614100:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_2:63005274-63005293 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_2:63005275-63005294 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_2:63005300-63005319 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_2:63005336-63005355 Msa0614100:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_2:63005565-63005584 None:intergenic 25.0%
! TTTGATTATATTTACCACAC+AGG - chr4_2:63006068-63006087 Msa0614100:intron 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_2:63006174-63006193 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_2:63006215-63006234 Msa0614100:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_2:63006268-63006287 Msa0614100:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_2:63006269-63006288 Msa0614100:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_2:63006363-63006382 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_2:63006375-63006394 Msa0614100:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_2:63006415-63006434 Msa0614100:intron 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_2:63006787-63006806 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_2:63006820-63006839 Msa0614100:five_prime_UTR 25.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_2:63004578-63004597 Msa0614100:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_2:63004595-63004614 Msa0614100:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_2:63004629-63004648 Msa0614100:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_2:63004737-63004756 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_2:63004781-63004800 Msa0614100:three_prime_UTR 30.0%
! TTAATTTGGTAGCCACAAAA+AGG - chr4_2:63004795-63004814 Msa0614100:three_prime_UTR 30.0%
! AAAAGTTGCATTCCTTTTTG+TGG + chr4_2:63004810-63004829 None:intergenic 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_2:63004922-63004941 Msa0614100:intron 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_2:63005089-63005108 Msa0614100:CDS 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_2:63005209-63005228 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_2:63005514-63005533 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_2:63005549-63005568 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_2:63005594-63005613 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_2:63005595-63005614 None:intergenic 30.0%
TATACTTGATCCAAACACAA+TGG + chr4_2:63005656-63005675 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_2:63005721-63005740 Msa0614100:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_2:63005826-63005845 Msa0614100:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_2:63005919-63005938 Msa0614100:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_2:63005922-63005941 Msa0614100:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_2:63006116-63006135 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_2:63006495-63006514 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_2:63006699-63006718 Msa0614100:intron 30.0%
AACTACAGTTATACAACCAA+AGG + chr4_2:63006725-63006744 None:intergenic 30.0%
CATATCGTATACATCAACTT+GGG + chr4_2:63006811-63006830 None:intergenic 30.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_2:63004578-63004597 Msa0614100:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_2:63004595-63004614 Msa0614100:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_2:63004629-63004648 Msa0614100:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_2:63004737-63004756 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_2:63004781-63004800 Msa0614100:three_prime_UTR 30.0%
! TTAATTTGGTAGCCACAAAA+AGG - chr4_2:63004795-63004814 Msa0614100:three_prime_UTR 30.0%
! AAAAGTTGCATTCCTTTTTG+TGG + chr4_2:63004810-63004829 None:intergenic 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_2:63004922-63004941 Msa0614100:intron 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_2:63005089-63005108 Msa0614100:CDS 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_2:63005209-63005228 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_2:63005514-63005533 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_2:63005549-63005568 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_2:63005594-63005613 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_2:63005595-63005614 None:intergenic 30.0%
TATACTTGATCCAAACACAA+TGG + chr4_2:63005656-63005675 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_2:63005721-63005740 Msa0614100:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_2:63005826-63005845 Msa0614100:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_2:63005919-63005938 Msa0614100:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_2:63005922-63005941 Msa0614100:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_2:63006116-63006135 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_2:63006495-63006514 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_2:63006699-63006718 Msa0614100:intron 30.0%
AACTACAGTTATACAACCAA+AGG + chr4_2:63006725-63006744 None:intergenic 30.0%
CATATCGTATACATCAACTT+GGG + chr4_2:63006811-63006830 None:intergenic 30.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_2:63004603-63004622 Msa0614100:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_2:63004725-63004744 Msa0614100:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_2:63004766-63004785 Msa0614100:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_2:63004891-63004910 Msa0614100:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_2:63005103-63005122 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_2:63005121-63005140 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_2:63005122-63005141 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_2:63005123-63005142 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_2:63005163-63005182 Msa0614100:intron 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_2:63005205-63005224 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_2:63005470-63005489 Msa0614100:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_2:63005515-63005534 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_2:63005536-63005555 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_2:63005533-63005552 Msa0614100:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_2:63005612-63005631 Msa0614100:intron 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_2:63005848-63005867 Msa0614100:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_2:63005878-63005897 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_2:63006047-63006066 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_2:63006074-63006093 Msa0614100:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_2:63006110-63006129 Msa0614100:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_2:63006196-63006215 Msa0614100:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_2:63006234-63006253 Msa0614100:intron 35.0%
AGACCTTATAAATGGACGAT+GGG - chr4_2:63006242-63006261 Msa0614100:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_2:63006561-63006580 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_2:63006812-63006831 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_2:63006809-63006828 Msa0614100:exon 35.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_2:63004603-63004622 Msa0614100:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_2:63004725-63004744 Msa0614100:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_2:63004766-63004785 Msa0614100:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_2:63004891-63004910 Msa0614100:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_2:63005103-63005122 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_2:63005121-63005140 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_2:63005122-63005141 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_2:63005123-63005142 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_2:63005163-63005182 Msa0614100:intron 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_2:63005205-63005224 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_2:63005470-63005489 Msa0614100:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_2:63005515-63005534 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_2:63005536-63005555 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_2:63005533-63005552 Msa0614100:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_2:63005612-63005631 Msa0614100:intron 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_2:63005848-63005867 Msa0614100:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_2:63005878-63005897 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_2:63006047-63006066 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_2:63006074-63006093 Msa0614100:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_2:63006110-63006129 Msa0614100:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_2:63006196-63006215 Msa0614100:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_2:63006234-63006253 Msa0614100:intron 35.0%
AGACCTTATAAATGGACGAT+GGG - chr4_2:63006242-63006261 Msa0614100:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_2:63006561-63006580 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_2:63006812-63006831 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_2:63006809-63006828 Msa0614100:exon 35.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_2:63004838-63004857 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_2:63004989-63005008 Msa0614100:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_2:63005002-63005021 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_2:63005000-63005019 Msa0614100:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_2:63005047-63005066 Msa0614100:intron 40.0%
!! TAAGAAGCACCTCTAGTTTC+TGG + chr4_2:63005059-63005078 None:intergenic 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_2:63005481-63005500 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_2:63005478-63005497 Msa0614100:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_2:63005622-63005641 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_2:63005623-63005642 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_2:63006243-63006262 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_2:63006241-63006260 Msa0614100:intron 40.0%
GACCTTATAAATGGACGATG+GGG - chr4_2:63006243-63006262 Msa0614100:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_2:63006324-63006343 Msa0614100:CDS 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_2:63006619-63006638 Msa0614100:intron 40.0%
GTTAAGGAAAATGAGGCCTT+TGG - chr4_2:63006706-63006725 Msa0614100:intron 40.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_2:63004838-63004857 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_2:63004989-63005008 Msa0614100:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_2:63005002-63005021 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_2:63005000-63005019 Msa0614100:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_2:63005047-63005066 Msa0614100:intron 40.0%
!! TAAGAAGCACCTCTAGTTTC+TGG + chr4_2:63005059-63005078 None:intergenic 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_2:63005481-63005500 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_2:63005478-63005497 Msa0614100:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_2:63005622-63005641 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_2:63005623-63005642 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_2:63006243-63006262 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_2:63006241-63006260 Msa0614100:intron 40.0%
GACCTTATAAATGGACGATG+GGG - chr4_2:63006243-63006262 Msa0614100:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_2:63006324-63006343 Msa0614100:CDS 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_2:63006619-63006638 Msa0614100:intron 40.0%
GTTAAGGAAAATGAGGCCTT+TGG - chr4_2:63006706-63006725 Msa0614100:intron 40.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_2:63004656-63004675 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_2:63004656-63004675 Msa0614100:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_2:63004660-63004679 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_2:63004999-63005018 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_2:63005201-63005220 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_2:63005263-63005282 Msa0614100:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_2:63005499-63005518 Msa0614100:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_2:63005502-63005521 Msa0614100:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_2:63005617-63005636 Msa0614100:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_2:63005618-63005637 Msa0614100:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_2:63005626-63005645 Msa0614100:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_2:63005627-63005646 Msa0614100:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_2:63005628-63005647 Msa0614100:intron 45.0%
GTCCCCATCGTCCATTTATA+AGG + chr4_2:63006248-63006267 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_2:63006300-63006319 Msa0614100:CDS 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_2:63006323-63006342 Msa0614100:CDS 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_2:63006325-63006344 Msa0614100:CDS 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_2:63006635-63006654 None:intergenic 45.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_2:63004656-63004675 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_2:63004656-63004675 Msa0614100:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_2:63004660-63004679 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_2:63004999-63005018 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_2:63005201-63005220 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_2:63005263-63005282 Msa0614100:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_2:63005499-63005518 Msa0614100:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_2:63005502-63005521 Msa0614100:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_2:63005617-63005636 Msa0614100:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_2:63005618-63005637 Msa0614100:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_2:63005626-63005645 Msa0614100:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_2:63005627-63005646 Msa0614100:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_2:63005628-63005647 Msa0614100:intron 45.0%
GTCCCCATCGTCCATTTATA+AGG + chr4_2:63006248-63006267 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_2:63006300-63006319 Msa0614100:CDS 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_2:63006323-63006342 Msa0614100:CDS 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_2:63006325-63006344 Msa0614100:CDS 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_2:63006635-63006654 None:intergenic 45.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_2:63006572-63006591 None:intergenic 5.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_2:63006572-63006591 None:intergenic 5.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_2:63004899-63004918 Msa0614100:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_2:63004900-63004919 Msa0614100:intron 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_2:63005197-63005216 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_2:63005629-63005648 Msa0614100:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_2:63005643-63005662 Msa0614100:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_2:63006085-63006104 None:intergenic 50.0%
TGGACGATGGGGACAATCAT+TGG - chr4_2:63006254-63006273 Msa0614100:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_2:63006543-63006562 Msa0614100:CDS 50.0%
TCATCCAGAGGACTGTGAGA+AGG - chr4_2:63006592-63006611 Msa0614100:intron 50.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_2:63004899-63004918 Msa0614100:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_2:63004900-63004919 Msa0614100:intron 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_2:63005197-63005216 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_2:63005629-63005648 Msa0614100:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_2:63005643-63005662 Msa0614100:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_2:63006085-63006104 None:intergenic 50.0%
TGGACGATGGGGACAATCAT+TGG - chr4_2:63006254-63006273 Msa0614100:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_2:63006543-63006562 Msa0614100:CDS 50.0%
TCATCCAGAGGACTGTGAGA+AGG - chr4_2:63006592-63006611 Msa0614100:intron 50.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_2:63006309-63006328 None:intergenic 55.0%
CTCTCCTTCTCACAGTCCTC+TGG + chr4_2:63006599-63006618 None:intergenic 55.0%
CAGAGGACTGTGAGAAGGAG+AGG - chr4_2:63006597-63006616 Msa0614100:intron 55.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_2:63006309-63006328 None:intergenic 55.0%
CTCTCCTTCTCACAGTCCTC+TGG + chr4_2:63006599-63006618 None:intergenic 55.0%
CAGAGGACTGTGAGAAGGAG+AGG - chr4_2:63006597-63006616 Msa0614100:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 63004535 63006875 63004535 ID=Msa0614100;Name=Msa0614100
chr4_2 mRNA 63004535 63006875 63004535 ID=Msa0614100-mRNA-1;Parent=Msa0614100;Name=Msa0614100-mRNA-1;_AED=0.17;_eAED=0.17;_QI=62|1|1|1|0.83|0.71|7|292|152
chr4_2 exon 63004535 63004845 63004535 ID=Msa0614100-mRNA-1:exon:17522;Parent=Msa0614100-mRNA-1
chr4_2 exon 63005064 63005173 63005064 ID=Msa0614100-mRNA-1:exon:17521;Parent=Msa0614100-mRNA-1
chr4_2 exon 63005279 63005320 63005279 ID=Msa0614100-mRNA-1:exon:17520;Parent=Msa0614100-mRNA-1
chr4_2 exon 63006126 63006167 63006126 ID=Msa0614100-mRNA-1:exon:17519;Parent=Msa0614100-mRNA-1
chr4_2 exon 63006300 63006399 63006300 ID=Msa0614100-mRNA-1:exon:17518;Parent=Msa0614100-mRNA-1
chr4_2 exon 63006498 63006562 63006498 ID=Msa0614100-mRNA-1:exon:17517;Parent=Msa0614100-mRNA-1
chr4_2 exon 63006733 63006875 63006733 ID=Msa0614100-mRNA-1:exon:17516;Parent=Msa0614100-mRNA-1
chr4_2 five_prime_UTR 63006814 63006875 63006814 ID=Msa0614100-mRNA-1:five_prime_utr;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63006733 63006813 63006733 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63006498 63006562 63006498 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63006300 63006399 63006300 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63006126 63006167 63006126 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63005279 63005320 63005279 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63005064 63005173 63005064 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 CDS 63004827 63004845 63004827 ID=Msa0614100-mRNA-1:cds;Parent=Msa0614100-mRNA-1
chr4_2 three_prime_UTR 63004535 63004826 63004535 ID=Msa0614100-mRNA-1:three_prime_utr;Parent=Msa0614100-mRNA-1
Gene Sequence

>Msa0614100

ATGGAACAAGTAATTGAAAGGCGTAATGGATATTCAGCAAATCACAGATTATTGGACCATCCATCTACTGATCAGCTTCAGGTTGAGAGCGACTCTACTTGCGACACACTGCGCAAGAAATTAGAAGATAAATCTCGTGAACTGAGGCAATTGAATGGAGAAGATCTGCAAGAATTGACAGTAGAAGAACTCCAGAAACTAGAGGTGCTTCTTAAAAGAAGTTTGTCTAGTGTTTCCAAAATAAAGGATGAAATGTTTATGCGAGACATCGACACCCTTAAGAGAAAGGAAGTGGAACTGATCGAAGAAAACCGAAGATTGAAACATGTGGTACCAGACCTTATAAATGGACGATGGGGACAATCATTGGAATCTGTTATTAGGGATTCATCTTTTTCTCCTGAAGACGACGATGGCAGTGACACTTCTCTCAAGTTGGGGTTACCATTTCTTAAATAA
Protein sequence

>Msa0614100

MEQVIERRNGYSANHRLLDHPSTDQLQVESDSTCDTLRKKLEDKSRELRQLNGEDLQELTVEELQKLEVLLKRSLSSVSKIKDEMFMRDIDTLKRKEVELIEENRRLKHVVPDLINGRWGQSLESVIRDSSFSPEDDDGSDTSLKLGLPFLK*