AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0697270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697270 MtrunA17_Chr4g0049901 90.196 153 13 2 1 152 88 239 2.51e-90 262
Msa0697270 MtrunA17_Chr5g0412461 41.212 165 81 3 1 150 62 225 4.14e-29 106
Msa0697270 MtrunA17_Chr5g0412271 42.169 166 79 3 1 150 62 226 5.14e-28 103
Msa0697270 MtrunA17_Chr5g0427401 33.939 165 91 3 1 150 62 223 2.32e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697270 AT2G22540.3 35.393 178 86 3 1 150 62 238 1.58e-21 87.4
Msa0697270 AT2G22540.1 35.393 178 86 3 1 150 62 238 1.58e-21 87.4
Msa0697270 AT2G22540.2 35.393 178 86 3 1 150 57 233 1.75e-21 87.0
Msa0697270 AT4G24540.1 40.252 159 82 6 1 149 62 217 4.98e-21 85.5

Find 44 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCATTGGAATCTGTTATT+AGG 0.132741 4_4:-67215195 Msa0697270:CDS
TGGGGAACAAACCTTTATTT+TGG 0.154101 4_4:+67216365 None:intergenic
TAAGAAGCGCCTCTAGTTTC+TGG 0.185828 4_4:+67216409 None:intergenic
TATACGATATGGTATGTTTA+TGG 0.191406 4_4:-67214633 Msa0697270:three_prime_UTR
TATCATTTACCTTTCTCTTA+AGG 0.249055 4_4:+67216188 None:intergenic
GACAGTATTGCTTCCTTTCT+AGG 0.249592 4_4:-67214830 Msa0697270:three_prime_UTR
TTCAGCAAATCACAGATTAT+TGG 0.255559 4_4:-67216836 Msa0697270:CDS
ATCATTGGAATCTGTTATTA+GGG 0.269501 4_4:-67215194 Msa0697270:CDS
ATCATTTACCTTTCTCTTAA+GGG 0.271180 4_4:+67216189 None:intergenic
GTCTAGTGTTTCCAAAATAA+AGG 0.316806 4_4:-67216376 Msa0697270:intron
GTCGCCATCGTCCATTTATA+AGG 0.317311 4_4:+67215218 None:intergenic
AGTGACACTTCTCTCAAGTT+GGG 0.338517 4_4:-67215139 Msa0697270:CDS
CCAAGTTGATGTATACGATA+TGG 0.348378 4_4:-67214644 Msa0697270:three_prime_UTR
TAGGTACCAGACCTTATAAA+TGG 0.357027 4_4:-67215229 Msa0697270:CDS
CTCTCCTTCTCACAGTCCTC+TGG 0.374200 4_4:+67214853 None:intergenic
TCACTGCCATCGTCGTCTTC+AGG 0.376609 4_4:+67215157 None:intergenic
TGGACGATGGCGACAATCAT+TGG 0.392563 4_4:-67215209 Msa0697270:CDS
TCCATCTACTGATCAGCTTC+AGG 0.394954 4_4:-67216809 Msa0697270:intron
TTACCACATGTTTCAATCTT+CGG 0.404641 4_4:+67215350 None:intergenic
GGTTAAGGAAAATGAGGCCT+TGG 0.425253 4_4:-67214747 Msa0697270:three_prime_UTR
CCATATCGTATACATCAACT+TGG 0.431123 4_4:+67214644 None:intergenic
TAAAGTAATAGATATGGTTA+AGG 0.446785 4_4:-67214762 Msa0697270:three_prime_UTR
GTAATGTAAAGTAATAGATA+TGG 0.448884 4_4:-67214768 Msa0697270:three_prime_UTR
CAAGTAATTGAAAGGCGTAA+TGG 0.461408 4_4:-67216862 Msa0697270:CDS
CAGTGACACTTCTCTCAAGT+TGG 0.471667 4_4:-67215140 Msa0697270:CDS
CATCGTCCATTTATAAGGTC+TGG 0.478047 4_4:+67215223 None:intergenic
CTTCAACACAGGCAATTGAA+TGG 0.479094 4_4:-67216465 Msa0697270:intron
GGTTGATCATCGACCTAGAA+AGG 0.492292 4_4:+67214817 None:intergenic
ACCTGAAGCTGATCAGTAGA+TGG 0.520990 4_4:+67216808 None:intergenic
CATCGACACCCTTAAGAGAA+AGG 0.526872 4_4:-67216197 Msa0697270:intron
CAGAGGACTGTGAGAAGGAG+AGG 0.553386 4_4:-67214852 Msa0697270:three_prime_UTR
AGATATGGTTAAGGAAAATG+AGG 0.554813 4_4:-67214753 Msa0697270:three_prime_UTR
ATAAATAAAATTTCATCCAG+AGG 0.569103 4_4:-67214869 Msa0697270:exon
GTATGGAACAAGTAATTGAA+AGG 0.588861 4_4:-67216870 Msa0697270:CDS
GTGACACTTCTCTCAAGTTG+GGG 0.597855 4_4:-67215138 Msa0697270:intron
TCATCCAGAGGACTGTGAGA+AGG 0.602265 4_4:-67214857 Msa0697270:three_prime_UTR
GAAGCTGATCAGTAGATGGA+TGG 0.615480 4_4:+67216812 None:intergenic
ATATATGTAAACACTTTCTG+TGG 0.627647 4_4:+67214669 None:intergenic
CATATCGTATACATCAACTT+GGG 0.647453 4_4:+67214645 None:intergenic
GAACTACAGTTATACAACCA+AGG 0.666830 4_4:+67214730 None:intergenic
AAGATAAATCTCGTGAACTG+AGG 0.669525 4_4:-67216574 Msa0697270:intron
AAACCGAAGATTGAAACATG+TGG 0.671175 4_4:-67215353 Msa0697270:intron
AGAAGAACTCCAGAAACTAG+AGG 0.688411 4_4:-67216418 Msa0697270:CDS
CAGACCTTATAAATGGACGA+TGG 0.740618 4_4:-67215222 Msa0697270:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTACTTTTTTTTTTTATAC+TGG + chr4_4:67216721-67216740 None:intergenic 10.0%
!!! ATTACTTTTTTTTTTTATAC+TGG + chr4_4:67216721-67216740 None:intergenic 10.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_4:67215375-67215394 Msa0697270:CDS 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_4:67215442-67215461 Msa0697270:intron 15.0%
!! ATGAATTCTATGTTAAATAA+AGG - chr4_4:67215832-67215851 Msa0697270:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_4:67215863-67215882 Msa0697270:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_4:67215864-67215883 Msa0697270:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_4:67215952-67215971 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_4:67215953-67215972 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_4:67216711-67216730 None:intergenic 15.0%
!! TTTGTTGGTATAATTTATAA+AGG - chr4_4:67215375-67215394 Msa0697270:CDS 15.0%
!!! ATTTTTAGACAAAAAAGTTA+AGG - chr4_4:67215442-67215461 Msa0697270:intron 15.0%
!! ATGAATTCTATGTTAAATAA+AGG - chr4_4:67215832-67215851 Msa0697270:intron 15.0%
!!! ATTTTGAATGTTTAAGATTT+AGG - chr4_4:67215863-67215882 Msa0697270:intron 15.0%
!!! TTTTGAATGTTTAAGATTTA+GGG - chr4_4:67215864-67215883 Msa0697270:intron 15.0%
!! ATTCTAAATGTCTATTTATT+GGG + chr4_4:67215952-67215971 None:intergenic 15.0%
!! TATTCTAAATGTCTATTTAT+TGG + chr4_4:67215953-67215972 None:intergenic 15.0%
!!! TTTTTTATACTGGATTTAAT+TGG + chr4_4:67216711-67216730 None:intergenic 15.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_4:67215351-67215370 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_4:67215360-67215379 Msa0697270:CDS 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_4:67216030-67216049 Msa0697270:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_4:67216203-67216222 Msa0697270:CDS 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_4:67216204-67216223 Msa0697270:CDS 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_4:67216457-67216476 Msa0697270:CDS 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_4:67216635-67216654 Msa0697270:intron 20.0%
!! GTAATGTAAAGTAATAGATA+TGG - chr4_4:67216736-67216755 Msa0697270:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_4:67216742-67216761 Msa0697270:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_4:67216803-67216822 Msa0697270:intron 20.0%
!!! TGATTTTTAAAAAGTATGAG+AGG + chr4_4:67215351-67215370 None:intergenic 20.0%
!! TTTAAAAATCACATCTTTGT+TGG - chr4_4:67215360-67215379 Msa0697270:CDS 20.0%
!!! TCATTCAATTTTATTTGACA+CGG - chr4_4:67216030-67216049 Msa0697270:intron 20.0%
!! TACAATTTGCCATATATATA+TGG - chr4_4:67216203-67216222 Msa0697270:CDS 20.0%
!! ACAATTTGCCATATATATAT+GGG - chr4_4:67216204-67216223 Msa0697270:CDS 20.0%
!!! ATCTCTCAAAAATTTAAGTT+GGG - chr4_4:67216457-67216476 Msa0697270:CDS 20.0%
!! ATAAATAAAATTTCATCCAG+AGG - chr4_4:67216635-67216654 Msa0697270:intron 20.0%
!! GTAATGTAAAGTAATAGATA+TGG - chr4_4:67216736-67216755 Msa0697270:intron 20.0%
!! TAAAGTAATAGATATGGTTA+AGG - chr4_4:67216742-67216761 Msa0697270:intron 20.0%
!!! CAATTTGTTCTATTTTCTTT+TGG - chr4_4:67216803-67216822 Msa0697270:intron 20.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_4:67214774-67214793 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_4:67214775-67214794 None:intergenic 25.0%
!!! TTAATTTGGTAGTGACAAAA+AGG - chr4_4:67214834-67214853 Msa0697270:three_prime_UTR 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_4:67214850-67214869 Msa0697270:three_prime_UTR 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_4:67214865-67214884 Msa0697270:three_prime_UTR 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_4:67215199-67215218 Msa0697270:CDS 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_4:67215265-67215284 Msa0697270:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_4:67215318-67215337 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_4:67215319-67215338 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_4:67215344-67215363 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_4:67215380-67215399 Msa0697270:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_4:67215609-67215628 None:intergenic 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_4:67216215-67216234 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_4:67216256-67216275 Msa0697270:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_4:67216309-67216328 Msa0697270:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_4:67216310-67216329 Msa0697270:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_4:67216404-67216423 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_4:67216416-67216435 Msa0697270:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_4:67216456-67216475 Msa0697270:CDS 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_4:67216838-67216857 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_4:67216871-67216890 Msa0697270:CDS 25.0%
! TAAAATTCCAGAATCAAAAG+GGG + chr4_4:67214774-67214793 None:intergenic 25.0%
! CTAAAATTCCAGAATCAAAA+GGG + chr4_4:67214775-67214794 None:intergenic 25.0%
!!! TTAATTTGGTAGTGACAAAA+AGG - chr4_4:67214834-67214853 Msa0697270:three_prime_UTR 25.0%
!!! AAAAAGGAATGCAACTTTTT+TGG - chr4_4:67214850-67214869 Msa0697270:three_prime_UTR 25.0%
!!! TTTTTTGGACCTTTATTTAC+AGG - chr4_4:67214865-67214884 Msa0697270:three_prime_UTR 25.0%
! AATACTGAAATCTTCTCATT+TGG - chr4_4:67215199-67215218 Msa0697270:CDS 25.0%
! ATAAGATATACTGATGTTTC+AGG - chr4_4:67215265-67215284 Msa0697270:intron 25.0%
! ATCATTTACCTTTCTCTTAA+GGG + chr4_4:67215318-67215337 None:intergenic 25.0%
! TATCATTTACCTTTCTCTTA+AGG + chr4_4:67215319-67215338 None:intergenic 25.0%
!!! TAAAAAGTATGAGAGGTTTT+TGG + chr4_4:67215344-67215363 None:intergenic 25.0%
! TGGTATAATTTATAAAGGTC+TGG - chr4_4:67215380-67215399 Msa0697270:intron 25.0%
! AGAAACAAAAGAAGTTAACA+TGG + chr4_4:67215609-67215628 None:intergenic 25.0%
! TTTATCGACCCATATATATA+TGG + chr4_4:67216215-67216234 None:intergenic 25.0%
!! ATGGAATACATGTGAATTTT+AGG - chr4_4:67216256-67216275 Msa0697270:intron 25.0%
! AATCATTGGAATCTGTTATT+AGG - chr4_4:67216309-67216328 Msa0697270:intron 25.0%
! ATCATTGGAATCTGTTATTA+GGG - chr4_4:67216310-67216329 Msa0697270:intron 25.0%
! AAGCATTACATGATAGTTAA+AGG + chr4_4:67216404-67216423 None:intergenic 25.0%
!! TAATGCTTACTTGTTAATGA+AGG - chr4_4:67216416-67216435 Msa0697270:CDS 25.0%
! CATCTCTCAAAAATTTAAGT+TGG - chr4_4:67216456-67216475 Msa0697270:CDS 25.0%
! ATATATGTAAACACTTTCTG+TGG + chr4_4:67216838-67216857 None:intergenic 25.0%
! TATACGATATGGTATGTTTA+TGG - chr4_4:67216871-67216890 Msa0697270:CDS 25.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_4:67214617-67214636 Msa0697270:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_4:67214634-67214653 Msa0697270:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_4:67214668-67214687 Msa0697270:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_4:67214776-67214795 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_4:67214820-67214839 Msa0697270:three_prime_UTR 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_4:67214961-67214980 Msa0697270:intron 30.0%
GTAGATATATGCACCTAATT+AGG + chr4_4:67214977-67214996 None:intergenic 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_4:67215128-67215147 Msa0697270:intron 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_4:67215253-67215272 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_4:67215558-67215577 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_4:67215593-67215612 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_4:67215638-67215657 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_4:67215639-67215658 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_4:67215769-67215788 Msa0697270:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_4:67215867-67215886 Msa0697270:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_4:67215960-67215979 Msa0697270:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_4:67215963-67215982 Msa0697270:intron 30.0%
TCTGATTATATTTACCACAC+AGG - chr4_4:67216109-67216128 Msa0697270:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_4:67216157-67216176 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_4:67216550-67216569 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_4:67216751-67216770 Msa0697270:intron 30.0%
CATATCGTATACATCAACTT+GGG + chr4_4:67216862-67216881 None:intergenic 30.0%
!! ATCATTGCTTGTTTTCAGTA+TGG - chr4_4:67214617-67214636 Msa0697270:three_prime_UTR 30.0%
GTATGGAACAAGTAATTGAA+AGG - chr4_4:67214634-67214653 Msa0697270:three_prime_UTR 30.0%
TTCAGCAAATCACAGATTAT+TGG - chr4_4:67214668-67214687 Msa0697270:three_prime_UTR 30.0%
GCTAAAATTCCAGAATCAAA+AGG + chr4_4:67214776-67214795 None:intergenic 30.0%
GATTGTGGTTGACATTAATT+TGG - chr4_4:67214820-67214839 Msa0697270:three_prime_UTR 30.0%
! GAGTACTTTTAGTCCTAATT+AGG - chr4_4:67214961-67214980 Msa0697270:intron 30.0%
GTAGATATATGCACCTAATT+AGG + chr4_4:67214977-67214996 None:intergenic 30.0%
GTCTAGTGTTTCCAAAATAA+AGG - chr4_4:67215128-67215147 Msa0697270:intron 30.0%
TATCTTATATGTCAGAAGCA+TGG + chr4_4:67215253-67215272 None:intergenic 30.0%
! GATATTTTTCCTCCTTATCA+GGG + chr4_4:67215558-67215577 None:intergenic 30.0%
AACATGGAAAACTCCAAAAT+TGG + chr4_4:67215593-67215612 None:intergenic 30.0%
ATTGTTAGATAAGACAGCAT+GGG + chr4_4:67215638-67215657 None:intergenic 30.0%
TATTGTTAGATAAGACAGCA+TGG + chr4_4:67215639-67215658 None:intergenic 30.0%
AGTGAGTGTAATTGACTAAT+TGG - chr4_4:67215769-67215788 Msa0697270:intron 30.0%
! TGAATGTTTAAGATTTAGGG+TGG - chr4_4:67215867-67215886 Msa0697270:intron 30.0%
! GACATTTAGAATAAATGCAG+TGG - chr4_4:67215960-67215979 Msa0697270:intron 30.0%
! ATTTAGAATAAATGCAGTGG+TGG - chr4_4:67215963-67215982 Msa0697270:intron 30.0%
TCTGATTATATTTACCACAC+AGG - chr4_4:67216109-67216128 Msa0697270:intron 30.0%
TTACCACATGTTTCAATCTT+CGG + chr4_4:67216157-67216176 None:intergenic 30.0%
AGAAACAGAAAAACTTTCTG+AGG + chr4_4:67216550-67216569 None:intergenic 30.0%
AGATATGGTTAAGGAAAATG+AGG - chr4_4:67216751-67216770 Msa0697270:intron 30.0%
CATATCGTATACATCAACTT+GGG + chr4_4:67216862-67216881 None:intergenic 30.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_4:67214642-67214661 Msa0697270:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_4:67214764-67214783 Msa0697270:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_4:67214805-67214824 Msa0697270:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_4:67214930-67214949 Msa0697270:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_4:67215142-67215161 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_4:67215160-67215179 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_4:67215161-67215180 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_4:67215162-67215181 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_4:67215207-67215226 Msa0697270:CDS 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_4:67215249-67215268 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_4:67215514-67215533 Msa0697270:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_4:67215559-67215578 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_4:67215580-67215599 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_4:67215577-67215596 Msa0697270:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_4:67215656-67215675 Msa0697270:intron 35.0%
CATACTTGATCCAAACACAA+TGG + chr4_4:67215700-67215719 None:intergenic 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_4:67215889-67215908 Msa0697270:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_4:67215919-67215938 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_4:67216088-67216107 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_4:67216115-67216134 Msa0697270:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_4:67216151-67216170 Msa0697270:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_4:67216237-67216256 Msa0697270:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_4:67216275-67216294 Msa0697270:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_4:67216616-67216635 None:intergenic 35.0%
GAACTACAGTTATACAACCA+AGG + chr4_4:67216777-67216796 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_4:67216863-67216882 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_4:67216860-67216879 Msa0697270:CDS 35.0%
! CAAGTAATTGAAAGGCGTAA+TGG - chr4_4:67214642-67214661 Msa0697270:three_prime_UTR 35.0%
!! ATGAAAACCCCTTTTGATTC+TGG - chr4_4:67214764-67214783 Msa0697270:three_prime_UTR 35.0%
CTAGTTATTCTGCATGATTG+TGG - chr4_4:67214805-67214824 Msa0697270:three_prime_UTR 35.0%
AAGATAAATCTCGTGAACTG+AGG - chr4_4:67214930-67214949 Msa0697270:intron 35.0%
!! TGGGGAACAAACCTTTATTT+TGG + chr4_4:67215142-67215161 None:intergenic 35.0%
ATTAGTGCACGATTAATCTG+GGG + chr4_4:67215160-67215179 None:intergenic 35.0%
GATTAGTGCACGATTAATCT+GGG + chr4_4:67215161-67215180 None:intergenic 35.0%
AGATTAGTGCACGATTAATC+TGG + chr4_4:67215162-67215181 None:intergenic 35.0%
AATCTTCTCATTTGGTTGTG+TGG - chr4_4:67215207-67215226 Msa0697270:CDS 35.0%
! TTATATGTCAGAAGCATGGA+TGG + chr4_4:67215249-67215268 None:intergenic 35.0%
ATACTGTTCCAATGTGATGT+TGG - chr4_4:67215514-67215533 Msa0697270:intron 35.0%
! GGATATTTTTCCTCCTTATC+AGG + chr4_4:67215559-67215578 None:intergenic 35.0%
CCAAAATTGGAGAATAACAC+TGG + chr4_4:67215580-67215599 None:intergenic 35.0%
!! CCAGTGTTATTCTCCAATTT+TGG - chr4_4:67215577-67215596 Msa0697270:intron 35.0%
ATAAGAGCCCTAATTCAACT+TGG - chr4_4:67215656-67215675 Msa0697270:intron 35.0%
CATACTTGATCCAAACACAA+TGG + chr4_4:67215700-67215719 None:intergenic 35.0%
GATGCATTGTCTTGATCTTT+CGG - chr4_4:67215889-67215908 Msa0697270:intron 35.0%
! CAACAAAGTGCTAGTGATTA+CGG + chr4_4:67215919-67215938 None:intergenic 35.0%
!! ACATTGTAGCAAGAGTGTTT+AGG + chr4_4:67216088-67216107 None:intergenic 35.0%
TATATTTACCACACAGGAAG+TGG - chr4_4:67216115-67216134 Msa0697270:intron 35.0%
AAACCGAAGATTGAAACATG+TGG - chr4_4:67216151-67216170 Msa0697270:intron 35.0%
CGCAGCAGCATAATAATATA+TGG - chr4_4:67216237-67216256 Msa0697270:intron 35.0%
TAGGTACCAGACCTTATAAA+TGG - chr4_4:67216275-67216294 Msa0697270:intron 35.0%
TTTAAGAAATGGTAACCTGC+AGG + chr4_4:67216616-67216635 None:intergenic 35.0%
GAACTACAGTTATACAACCA+AGG + chr4_4:67216777-67216796 None:intergenic 35.0%
CCATATCGTATACATCAACT+TGG + chr4_4:67216863-67216882 None:intergenic 35.0%
!! CCAAGTTGATGTATACGATA+TGG - chr4_4:67216860-67216879 Msa0697270:CDS 35.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_4:67214877-67214896 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_4:67215028-67215047 Msa0697270:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_4:67215041-67215060 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_4:67215039-67215058 Msa0697270:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_4:67215086-67215105 Msa0697270:intron 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_4:67215525-67215544 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_4:67215522-67215541 Msa0697270:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_4:67215666-67215685 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_4:67215667-67215686 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_4:67216284-67216303 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_4:67216282-67216301 Msa0697270:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_4:67216365-67216384 Msa0697270:intron 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_4:67216674-67216693 Msa0697270:intron 40.0%
CGCTCTCAACCTGTAAATAA+AGG + chr4_4:67214877-67214896 None:intergenic 40.0%
AGTAATTCCACCTTCAACAC+AGG - chr4_4:67215028-67215047 Msa0697270:intron 40.0%
CATTCAATTGCCTGTGTTGA+AGG + chr4_4:67215041-67215060 None:intergenic 40.0%
CTTCAACACAGGCAATTGAA+TGG - chr4_4:67215039-67215058 Msa0697270:intron 40.0%
AGAAGAACTCCAGAAACTAG+AGG - chr4_4:67215086-67215105 Msa0697270:intron 40.0%
CCACTTAACCAACATCACAT+TGG + chr4_4:67215525-67215544 None:intergenic 40.0%
! CCAATGTGATGTTGGTTAAG+TGG - chr4_4:67215522-67215541 Msa0697270:intron 40.0%
! TCCCTCACCAAGTTGAATTA+GGG + chr4_4:67215666-67215685 None:intergenic 40.0%
! TTCCCTCACCAAGTTGAATT+AGG + chr4_4:67215667-67215686 None:intergenic 40.0%
CATCGTCCATTTATAAGGTC+TGG + chr4_4:67216284-67216303 None:intergenic 40.0%
CAGACCTTATAAATGGACGA+TGG - chr4_4:67216282-67216301 Msa0697270:intron 40.0%
AGTGACACTTCTCTCAAGTT+GGG - chr4_4:67216365-67216384 Msa0697270:intron 40.0%
GACAGTATTGCTTCCTTTCT+AGG - chr4_4:67216674-67216693 Msa0697270:intron 40.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_4:67214695-67214714 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_4:67214695-67214714 Msa0697270:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_4:67214699-67214718 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_4:67215038-67215057 None:intergenic 45.0%
!! TAAGAAGCGCCTCTAGTTTC+TGG + chr4_4:67215098-67215117 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_4:67215245-67215264 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_4:67215307-67215326 Msa0697270:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_4:67215543-67215562 Msa0697270:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_4:67215546-67215565 Msa0697270:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_4:67215661-67215680 Msa0697270:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_4:67215662-67215681 Msa0697270:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_4:67215670-67215689 Msa0697270:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_4:67215671-67215690 Msa0697270:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_4:67215672-67215691 Msa0697270:intron 45.0%
GTCGCCATCGTCCATTTATA+AGG + chr4_4:67216289-67216308 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_4:67216341-67216360 Msa0697270:intron 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_4:67216364-67216383 Msa0697270:intron 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_4:67216366-67216385 Msa0697270:intron 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_4:67216690-67216709 None:intergenic 45.0%
GGTTAAGGAAAATGAGGCCT+TGG - chr4_4:67216757-67216776 Msa0697270:intron 45.0%
GAAGCTGATCAGTAGATGGA+TGG + chr4_4:67214695-67214714 None:intergenic 45.0%
TCCATCTACTGATCAGCTTC+AGG - chr4_4:67214695-67214714 Msa0697270:three_prime_UTR 45.0%
ACCTGAAGCTGATCAGTAGA+TGG + chr4_4:67214699-67214718 None:intergenic 45.0%
TCAATTGCCTGTGTTGAAGG+TGG + chr4_4:67215038-67215057 None:intergenic 45.0%
!! TAAGAAGCGCCTCTAGTTTC+TGG + chr4_4:67215098-67215117 None:intergenic 45.0%
! ATGTCAGAAGCATGGATGGA+TGG + chr4_4:67215245-67215264 None:intergenic 45.0%
CATCGACACCCTTAAGAGAA+AGG - chr4_4:67215307-67215326 Msa0697270:intron 45.0%
GGAAGCAATTTGCCCTGATA+AGG - chr4_4:67215543-67215562 Msa0697270:intron 45.0%
AGCAATTTGCCCTGATAAGG+AGG - chr4_4:67215546-67215565 Msa0697270:intron 45.0%
! AGCCCTAATTCAACTTGGTG+AGG - chr4_4:67215661-67215680 Msa0697270:intron 45.0%
! GCCCTAATTCAACTTGGTGA+GGG - chr4_4:67215662-67215681 Msa0697270:intron 45.0%
! TCAACTTGGTGAGGGAATGT+AGG - chr4_4:67215670-67215689 Msa0697270:intron 45.0%
! CAACTTGGTGAGGGAATGTA+GGG - chr4_4:67215671-67215690 Msa0697270:intron 45.0%
! AACTTGGTGAGGGAATGTAG+GGG - chr4_4:67215672-67215691 Msa0697270:intron 45.0%
GTCGCCATCGTCCATTTATA+AGG + chr4_4:67216289-67216308 None:intergenic 45.0%
! TTTTCTCCTGAAGACGACGA+TGG - chr4_4:67216341-67216360 Msa0697270:intron 45.0%
CAGTGACACTTCTCTCAAGT+TGG - chr4_4:67216364-67216383 Msa0697270:intron 45.0%
! GTGACACTTCTCTCAAGTTG+GGG - chr4_4:67216366-67216385 Msa0697270:intron 45.0%
GGTTGATCATCGACCTAGAA+AGG + chr4_4:67216690-67216709 None:intergenic 45.0%
GGTTAAGGAAAATGAGGCCT+TGG - chr4_4:67216757-67216776 Msa0697270:intron 45.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_4:67216627-67216646 None:intergenic 5.0%
!!! AATTTTATTTATTTAAGAAA+TGG + chr4_4:67216627-67216646 None:intergenic 5.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_4:67214938-67214957 Msa0697270:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_4:67214939-67214958 Msa0697270:intron 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_4:67215241-67215260 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_4:67215673-67215692 Msa0697270:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_4:67215687-67215706 Msa0697270:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_4:67216126-67216145 None:intergenic 50.0%
TGGACGATGGCGACAATCAT+TGG - chr4_4:67216295-67216314 Msa0697270:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_4:67216598-67216617 Msa0697270:CDS 50.0%
TCATCCAGAGGACTGTGAGA+AGG - chr4_4:67216647-67216666 Msa0697270:intron 50.0%
TCTCGTGAACTGAGGTACAG+TGG - chr4_4:67214938-67214957 Msa0697270:intron 50.0%
CTCGTGAACTGAGGTACAGT+GGG - chr4_4:67214939-67214958 Msa0697270:intron 50.0%
! CAGAAGCATGGATGGATGGA+TGG + chr4_4:67215241-67215260 None:intergenic 50.0%
! ACTTGGTGAGGGAATGTAGG+GGG - chr4_4:67215673-67215692 Msa0697270:intron 50.0%
TGTAGGGGGACCATTGTGTT+TGG - chr4_4:67215687-67215706 Msa0697270:intron 50.0%
GATCAGTTCCACTTCCTGTG+TGG + chr4_4:67216126-67216145 None:intergenic 50.0%
TGGACGATGGCGACAATCAT+TGG - chr4_4:67216295-67216314 Msa0697270:intron 50.0%
TGAGTTCATCTGTGTCCTGC+AGG - chr4_4:67216598-67216617 Msa0697270:CDS 50.0%
TCATCCAGAGGACTGTGAGA+AGG - chr4_4:67216647-67216666 Msa0697270:intron 50.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_4:67216350-67216369 None:intergenic 55.0%
CTCTCCTTCTCACAGTCCTC+TGG + chr4_4:67216654-67216673 None:intergenic 55.0%
CAGAGGACTGTGAGAAGGAG+AGG - chr4_4:67216652-67216671 Msa0697270:intron 55.0%
TCACTGCCATCGTCGTCTTC+AGG + chr4_4:67216350-67216369 None:intergenic 55.0%
CTCTCCTTCTCACAGTCCTC+TGG + chr4_4:67216654-67216673 None:intergenic 55.0%
CAGAGGACTGTGAGAAGGAG+AGG - chr4_4:67216652-67216671 Msa0697270:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 67214600 67216926 67214600 ID=Msa0697270;Name=Msa0697270
chr4_4 mRNA 67214600 67216926 67214600 ID=Msa0697270-mRNA-1;Parent=Msa0697270;Name=Msa0697270-mRNA-1;_AED=0.07;_eAED=0.07;_QI=36|1|1|1|0.83|0.71|7|288|152
chr4_4 exon 67214600 67214906 67214600 ID=Msa0697270-mRNA-1:exon:19756;Parent=Msa0697270-mRNA-1
chr4_4 exon 67215139 67215248 67215139 ID=Msa0697270-mRNA-1:exon:19755;Parent=Msa0697270-mRNA-1
chr4_4 exon 67215354 67215395 67215354 ID=Msa0697270-mRNA-1:exon:19754;Parent=Msa0697270-mRNA-1
chr4_4 exon 67216198 67216239 67216198 ID=Msa0697270-mRNA-1:exon:19753;Parent=Msa0697270-mRNA-1
chr4_4 exon 67216377 67216476 67216377 ID=Msa0697270-mRNA-1:exon:19752;Parent=Msa0697270-mRNA-1
chr4_4 exon 67216575 67216639 67216575 ID=Msa0697270-mRNA-1:exon:19751;Parent=Msa0697270-mRNA-1
chr4_4 exon 67216810 67216926 67216810 ID=Msa0697270-mRNA-1:exon:19750;Parent=Msa0697270-mRNA-1
chr4_4 five_prime_UTR 67216891 67216926 67216891 ID=Msa0697270-mRNA-1:five_prime_utr;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67216810 67216890 67216810 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67216575 67216639 67216575 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67216377 67216476 67216377 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67216198 67216239 67216198 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67215354 67215395 67215354 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67215139 67215248 67215139 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 CDS 67214888 67214906 67214888 ID=Msa0697270-mRNA-1:cds;Parent=Msa0697270-mRNA-1
chr4_4 three_prime_UTR 67214600 67214887 67214600 ID=Msa0697270-mRNA-1:three_prime_utr;Parent=Msa0697270-mRNA-1
Gene Sequence

>Msa0697270

ATGGAACAAGTAATTGAAAGGCGTAATGGATATTCAGCAAATCACAGATTATTGGACCATCCATCTACTGATCAGCTTCAGGTTGAGAGCGACTCTACTTGCGACACACTGCGCAAGAAATTAGAAGATAAATCTCGTGAACTGAGGCAATTGAATGGAGAAGATCTGCAAGAATTGACAGTAGAAGAACTCCAGAAACTAGAGGCGCTTCTTAAAAGAAGTTTGTCTAGTGTTTCCAAAATAAAGGATGAAATGTTTATGCGAGACATCGACACCCTTAAGAGAAAGGAAGTGGAACTGATCGAAGAAAACCGAAGATTGAAACATGTGGTACCAGACCTTATAAATGGACGATGGCGACAATCATTGGAATCTGTTATTAGGGATTCATCTTTTTCTCCTGAAGACGACGATGGCAGTGACACTTCTCTCAAGTTGGGGTTACCATTTCTTAAATAA
Protein sequence

>Msa0697270

MEQVIERRNGYSANHRLLDHPSTDQLQVESDSTCDTLRKKLEDKSRELRQLNGEDLQELTVEELQKLEALLKRSLSSVSKIKDEMFMRDIDTLKRKEVELIEENRRLKHVVPDLINGRWRQSLESVIRDSSFSPEDDDGSDTSLKLGLPFLK*