AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614810 MtrunA17_Chr4g0036991 68.103 116 33 1 201 312 493 608 2.29e-42 155
Msa0614810 MtrunA17_Chr4g0036991 50.000 146 42 5 36 156 89 228 8.31e-29 116
Msa0614810 MtrunA17_Chr4g0036991 59.259 81 29 2 161 239 321 399 4.07e-20 91.3
Msa0614810 MtrunA17_Chr1g0158701 70.909 55 16 0 187 241 22 76 8.00e-20 85.1
Msa0614810 MtrunA17_Chr1g0157811 58.571 70 27 1 233 300 414 483 1.41e-18 86.7
Msa0614810 MtrunA17_Chr1g0157811 65.455 55 19 0 187 241 233 287 2.15e-16 80.1
Msa0614810 MtrunA17_Chr1g0160701 64.407 59 21 0 187 245 378 436 1.76e-18 86.3
Msa0614810 MtrunA17_Chr1g0160701 45.714 70 36 1 233 300 560 629 8.56e-12 66.2
Msa0614810 MtrunA17_Chr1g0158191 55.263 76 32 1 227 300 599 674 1.79e-18 86.3
Msa0614810 MtrunA17_Chr1g0158191 60.000 55 22 0 187 241 424 478 2.47e-14 73.9
Msa0614810 MtrunA17_Chr1g0157951 58.571 70 27 1 233 300 395 464 1.95e-18 85.9
Msa0614810 MtrunA17_Chr1g0157951 65.455 55 19 0 187 241 214 268 5.78e-16 78.6
Msa0614810 MtrunA17_Chr1g0157791 58.571 70 27 1 233 300 601 670 2.34e-18 86.3
Msa0614810 MtrunA17_Chr1g0157791 65.455 55 19 0 187 241 420 474 3.26e-16 79.7
Msa0614810 MtrunA17_Chr1g0158761 57.143 70 28 1 233 300 549 618 4.00e-18 85.5
Msa0614810 MtrunA17_Chr1g0158761 69.091 55 17 0 187 241 368 422 2.75e-17 82.8
Msa0614810 MtrunA17_Chr1g0158151 55.714 70 29 1 233 300 600 669 1.30e-17 84.0
Msa0614810 MtrunA17_Chr1g0158151 65.455 55 19 0 187 241 419 473 3.19e-16 79.7
Msa0614810 MtrunA17_Chr1g0160531 62.069 58 22 0 187 244 157 214 1.57e-17 83.2
Msa0614810 MtrunA17_Chr1g0160531 40.506 79 38 2 233 302 338 416 1.68e-11 65.1
Msa0614810 MtrunA17_Chr1g0157911 51.807 83 38 1 158 238 401 483 2.02e-17 83.2
Msa0614810 MtrunA17_Chr1g0157911 47.887 71 34 1 233 300 613 683 1.29e-12 68.6
Msa0614810 MtrunA17_Chr1g0158411 65.574 61 20 1 187 247 438 497 2.73e-17 82.8
Msa0614810 MtrunA17_Chr1g0158411 40.000 100 53 3 233 325 619 718 5.22e-14 72.8
Msa0614810 MtrunA17_Chr1g0160661 61.017 59 23 0 187 245 385 443 4.07e-17 82.0
Msa0614810 MtrunA17_Chr1g0158091 65.455 55 19 0 187 241 105 159 7.01e-17 80.9
Msa0614810 MtrunA17_Chr1g0158091 54.286 70 30 1 233 300 286 355 8.14e-13 68.6
Msa0614810 MtrunA17_Chr6g0467711 71.154 52 15 0 187 238 434 485 8.21e-17 81.3
Msa0614810 MtrunA17_Chr6g0467711 50.000 70 33 1 233 300 615 684 2.68e-14 73.9
Msa0614810 MtrunA17_Chr1g0158751 65.455 55 19 0 187 241 129 183 1.00e-16 80.5
Msa0614810 MtrunA17_Chr1g0158751 39.175 97 53 2 233 323 310 406 2.59e-13 70.5
Msa0614810 MtrunA17_Chr3g0088321 40.541 111 64 1 128 238 29 137 1.06e-16 80.1
Msa0614810 MtrunA17_Chr3g0088321 48.571 70 34 1 233 300 267 336 6.25e-14 72.0
Msa0614810 MtrunA17_Chr1g0158131 65.455 55 19 0 187 241 237 291 1.58e-16 80.1
Msa0614810 MtrunA17_Chr1g0160711 60.345 58 23 0 187 244 361 418 1.95e-16 80.1
Msa0614810 MtrunA17_Chr1g0158571 49.412 85 40 2 187 269 246 329 2.04e-16 80.1
Msa0614810 MtrunA17_Chr1g0158571 47.887 71 34 2 233 300 427 497 1.69e-12 68.2
Msa0614810 MtrunA17_Chr1g0160761 60.345 58 23 0 187 244 368 425 3.16e-16 79.7
Msa0614810 MtrunA17_Chr1g0160721 60.345 58 23 0 187 244 384 441 3.42e-16 79.7
Msa0614810 MtrunA17_Chr1g0160721 44.286 70 37 1 233 300 566 635 1.96e-12 68.2
Msa0614810 MtrunA17_Chr1g0160561 48.235 85 40 2 163 244 353 436 3.64e-16 79.3
Msa0614810 MtrunA17_Chr1g0160561 43.038 79 36 3 233 302 561 639 1.76e-11 65.1
Msa0614810 MtrunA17_Chr1g0158441 61.905 63 23 1 187 249 62 123 4.91e-16 78.2
Msa0614810 MtrunA17_Chr1g0158441 38.542 96 54 2 233 323 243 338 1.16e-12 68.2
Msa0614810 MtrunA17_Chr1g0158631 59.375 64 25 1 187 250 417 479 5.46e-16 79.0
Msa0614810 MtrunA17_Chr1g0158631 28.899 218 119 8 97 300 473 668 2.59e-12 67.8
Msa0614810 MtrunA17_Chr1g0158541 64.407 59 19 1 190 248 432 488 6.47e-16 78.6
Msa0614810 MtrunA17_Chr1g0158541 52.113 71 31 1 233 300 610 680 7.57e-16 78.6
Msa0614810 MtrunA17_Chr7g0232421 60.000 55 22 0 187 241 96 150 9.19e-16 77.4
Msa0614810 MtrunA17_Chr1g0158301 67.308 52 17 0 190 241 430 481 9.53e-16 78.2
Msa0614810 MtrunA17_Chr1g0158301 50.704 71 32 1 233 300 608 678 4.16e-15 76.3
Msa0614810 MtrunA17_Chr1g0157991 52.857 70 31 1 233 300 608 677 1.28e-15 77.8
Msa0614810 MtrunA17_Chr1g0157991 60.000 55 22 0 187 241 427 481 6.90e-15 75.5
Msa0614810 MtrunA17_Chr1g0158661 63.636 55 20 0 187 241 403 457 1.67e-15 77.4
Msa0614810 MtrunA17_Chr1g0158661 43.662 71 37 1 233 300 584 654 9.08e-11 63.2
Msa0614810 MtrunA17_Chr1g0158041 63.636 55 20 0 187 241 424 478 1.67e-15 77.4
Msa0614810 MtrunA17_Chr1g0158041 51.429 70 32 1 233 300 605 674 7.91e-15 75.5
Msa0614810 MtrunA17_Chr1g0158341 54.286 70 30 1 233 300 243 312 1.79e-15 76.3
Msa0614810 MtrunA17_Chr1g0158341 60.000 60 23 1 187 246 62 120 4.08e-14 72.4
Msa0614810 MtrunA17_Chr1g0209411 58.182 55 23 0 187 241 327 381 2.16e-15 76.6
Msa0614810 MtrunA17_Chr7g0232441 60.000 55 22 0 187 241 356 410 2.31e-15 77.0
Msa0614810 MtrunA17_Chr1g0209471 59.259 54 22 0 188 241 97 150 2.79e-15 75.9
Msa0614810 MtrunA17_Chr1g0160681 59.259 54 22 0 185 238 96 149 3.03e-15 75.9
Msa0614810 MtrunA17_Chr6g0484041 46.512 86 43 1 187 269 356 441 3.78e-15 76.3
Msa0614810 MtrunA17_Chr6g0484021 45.349 86 44 1 187 269 340 425 6.15e-15 75.5
Msa0614810 MtrunA17_Chr6g0483981 45.349 86 44 1 187 269 301 386 9.51e-15 75.1
Msa0614810 MtrunA17_Chr1g0158141 55.172 58 26 0 243 300 7 64 1.17e-14 69.3
Msa0614810 MtrunA17_Chr1g0160771 61.538 52 20 0 187 238 334 385 1.21e-14 74.7
Msa0614810 MtrunA17_Chr1g0209481 58.182 55 23 0 187 241 241 295 1.69e-14 73.9
Msa0614810 MtrunA17_Chr1g0160581 59.615 52 21 0 187 238 338 389 2.12e-14 73.9
Msa0614810 MtrunA17_Chr6g0483851 45.349 86 44 1 187 269 444 529 2.49e-14 73.9
Msa0614810 MtrunA17_Chr6g0484071 58.333 60 25 0 187 246 92 151 2.49e-14 73.2
Msa0614810 MtrunA17_Chr1g0157861 65.306 49 17 0 190 238 471 519 2.63e-14 73.9
Msa0614810 MtrunA17_Chr1g0157881 63.265 49 18 0 190 238 502 550 3.26e-14 73.6
Msa0614810 MtrunA17_Chr1g0157881 49.296 71 33 2 233 300 680 750 5.79e-12 66.6
Msa0614810 MtrunA17_Chr6g0483821 58.333 60 25 0 187 246 393 452 3.82e-14 73.2
Msa0614810 MtrunA17_Chr1g0157931 66.667 48 16 0 191 238 310 357 4.07e-14 73.2
Msa0614810 MtrunA17_Chr1g0157931 49.296 71 33 2 233 300 487 557 4.16e-12 67.0
Msa0614810 MtrunA17_Chr1g0209401 56.364 55 24 0 187 241 87 141 9.16e-14 71.2
Msa0614810 MtrunA17_Chr1g0160631 37.755 98 59 1 233 328 97 194 1.85e-13 68.2
Msa0614810 MtrunA17_Chr6g0483791 45.349 86 44 1 187 269 403 488 1.93e-13 71.2
Msa0614810 MtrunA17_Chr6g0483791 42.466 73 39 1 231 300 583 655 9.20e-11 63.2
Msa0614810 MtrunA17_Chr1g0160691 47.143 70 35 1 233 300 18 87 3.00e-13 65.9
Msa0614810 MtrunA17_Chr7g0251001 37.374 99 58 2 143 239 48 144 3.99e-13 69.7
Msa0614810 MtrunA17_Chr1g0160731 55.769 52 23 0 187 238 330 381 4.06e-13 70.1
Msa0614810 MtrunA17_Chr6g0483811 43.023 86 46 1 187 269 92 177 4.26e-13 69.3
Msa0614810 MtrunA17_Chr6g0483811 42.466 73 39 1 231 300 272 344 5.24e-11 63.2
Msa0614810 MtrunA17_Chr8g0391231 45.122 82 43 1 167 248 350 429 5.83e-13 69.7
Msa0614810 MtrunA17_Chr7g0250741 55.769 52 23 0 188 239 568 619 8.58e-13 69.3
Msa0614810 MtrunA17_Chr7g0251061 43.836 73 39 1 167 239 363 433 9.07e-13 69.3
Msa0614810 MtrunA17_Chr1g0158481 39.000 100 54 3 233 325 140 239 1.16e-12 67.0
Msa0614810 MtrunA17_Chr6g0467641 57.143 49 21 0 190 238 229 277 2.42e-12 67.8
Msa0614810 MtrunA17_Chr6g0484091 47.458 59 31 0 187 245 36 94 3.21e-12 62.0
Msa0614810 MtrunA17_Chr1g0158391 41.379 87 47 2 154 238 257 341 3.48e-12 67.4
Msa0614810 MtrunA17_Chr6g0467701 57.143 49 21 0 190 238 70 118 4.46e-12 66.2
Msa0614810 MtrunA17_Chr1g0158351 51.923 52 25 0 187 238 5 56 5.58e-12 64.3
Msa0614810 MtrunA17_Chr1g0212821 43.662 71 37 1 233 300 299 369 6.30e-12 66.2
Msa0614810 MtrunA17_Chr1g0212821 48.333 60 30 1 188 246 116 175 6.20e-11 63.2
Msa0614810 MtrunA17_Chr1g0158321 55.102 49 22 0 190 238 70 118 6.30e-12 65.9
Msa0614810 MtrunA17_Chr7g0251051 39.080 87 49 2 187 269 346 432 6.56e-12 66.6
Msa0614810 MtrunA17_Chr7g0251101 41.096 73 41 1 167 239 374 444 7.60e-12 66.2
Msa0614810 MtrunA17_Chr7g0251101 40.845 71 39 1 233 300 576 646 1.84e-11 65.1
Msa0614810 MtrunA17_Chr3g0088341 41.379 87 47 2 154 238 278 362 7.88e-12 66.2
Msa0614810 MtrunA17_Chr6g0467681 57.143 49 21 0 190 238 300 348 8.26e-12 66.2
Msa0614810 MtrunA17_Chr7g0251071 52.830 53 25 0 187 239 386 438 9.59e-12 65.9
Msa0614810 MtrunA17_Chr1g0157901 53.846 52 24 0 187 238 5 56 9.95e-12 64.7
Msa0614810 MtrunA17_Chr1g0158641 58.824 51 19 1 233 281 42 92 1.14e-11 60.5
Msa0614810 MtrunA17_Chr4g0022331 41.379 87 47 2 188 270 566 652 1.19e-11 65.9
Msa0614810 MtrunA17_Chr1g0158311 62.222 45 17 0 187 231 321 365 1.80e-11 65.1
Msa0614810 MtrunA17_Chr1g0158531 43.210 81 40 2 161 238 297 374 1.96e-11 65.1
Msa0614810 MtrunA17_Chr1g0158511 51.923 52 25 0 187 238 311 362 2.03e-11 65.1
Msa0614810 MtrunA17_Chr1g0157941 57.143 49 21 0 190 238 295 343 2.39e-11 64.7
Msa0614810 MtrunA17_Chr6g0484051 41.429 70 39 1 233 300 565 634 2.53e-11 64.7
Msa0614810 MtrunA17_Chr6g0484051 49.153 59 30 0 187 245 382 440 3.11e-11 64.3
Msa0614810 MtrunA17_Chr7g0251081 40.845 71 39 1 233 300 326 396 2.65e-11 64.3
Msa0614810 MtrunA17_Chr1g0158771 45.714 70 36 1 233 300 504 573 2.72e-11 64.7
Msa0614810 MtrunA17_Chr1g0209501 46.667 60 32 0 188 247 343 402 3.29e-11 64.3
Msa0614810 MtrunA17_Chr6g0467661 55.102 49 22 0 190 238 300 348 3.70e-11 63.9
Msa0614810 MtrunA17_Chr1g0157871 57.143 49 21 0 190 238 297 345 3.90e-11 63.9
Msa0614810 MtrunA17_Chr1g0158471 55.102 49 22 0 190 238 314 362 3.92e-11 63.9
Msa0614810 MtrunA17_Chr7g0253471 42.857 70 38 1 233 300 99 168 3.97e-11 61.2
Msa0614810 MtrunA17_Chr4g0022301 38.028 71 41 1 233 300 57 127 5.51e-11 59.7
Msa0614810 MtrunA17_Chr1g0175451 48.276 58 30 0 187 244 362 419 5.68e-11 63.5
Msa0614810 MtrunA17_Chr6g0484101 39.437 71 40 1 233 300 24 94 8.23e-11 58.5
Msa0614810 MtrunA17_Chr1g0158611 50.000 52 26 0 187 238 84 135 9.62e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614810 AT5G38280.1 45.833 72 35 1 233 300 547 618 4.96e-14 73.6
Msa0614810 AT5G38280.1 35.714 84 54 0 187 270 365 448 2.73e-12 68.2
Msa0614810 AT5G38280.2 45.833 72 35 1 233 300 547 618 5.08e-14 73.6
Msa0614810 AT5G38280.2 35.714 84 54 0 187 270 365 448 3.41e-12 67.8
Msa0614810 AT5G39030.1 41.304 92 45 2 233 315 713 804 1.11e-13 72.4
Msa0614810 AT5G39030.2 41.304 92 45 2 233 315 707 798 1.28e-13 72.4
Msa0614810 AT5G38240.1 38.947 95 45 2 233 323 544 629 8.34e-13 69.7
Msa0614810 AT5G38250.1 38.462 104 48 3 230 326 517 611 8.49e-13 69.7
Msa0614810 AT5G38240.2 38.947 95 45 2 233 323 526 611 8.52e-13 69.7
Msa0614810 AT5G38240.3 38.947 95 45 2 233 323 480 565 9.62e-13 69.3
Msa0614810 AT1G67000.1 44.444 63 35 0 189 251 592 654 6.02e-12 67.0
Msa0614810 AT1G67000.1 34.821 112 59 2 233 332 773 882 1.98e-11 65.5
Msa0614810 AT4G29050.1 34.091 88 58 0 185 272 377 464 1.27e-11 66.2
Msa0614810 AT4G29050.2 34.091 88 58 0 185 272 391 478 1.32e-11 65.9
Msa0614810 AT1G70130.1 32.955 88 59 0 185 272 367 454 1.85e-11 65.5
Msa0614810 AT1G66910.2 47.541 61 32 0 187 247 378 438 2.45e-11 65.1
Msa0614810 AT1G66980.1 44.262 61 34 0 187 247 838 898 2.68e-11 65.5
Msa0614810 AT1G66980.2 44.262 61 34 0 187 247 867 927 2.69e-11 65.5
Msa0614810 AT1G66980.4 44.262 61 34 0 187 247 821 881 2.74e-11 65.1
Msa0614810 AT1G70250.1 51.923 52 25 0 187 238 493 544 3.05e-11 65.1
Msa0614810 AT1G66980.3 44.262 61 34 0 187 247 865 925 3.08e-11 65.1
Msa0614810 AT1G66910.1 47.541 61 32 0 187 247 381 441 3.19e-11 64.7
Msa0614810 AT1G66920.2 48.276 58 30 0 190 247 344 401 9.26e-11 63.5

Find 74 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAGATTACTTTGCTAAA+TGG 0.212590 4_2:+63944709 Msa0614810:intron
GTCAATGTGGTGCAATTAAT+TGG 0.230216 4_2:+63944559 Msa0614810:CDS
ATTCATCGAGGGCCGGTCAT+TGG 0.272372 4_2:-63944841 None:intergenic
ACCTAATCATGAAGAATATA+TGG 0.312558 4_2:+63943594 Msa0614810:CDS
TGGATCAGGTGGAAGGTATT+GGG 0.315917 4_2:-63944898 None:intergenic
ACCATATATTCTTCATGATT+AGG 0.344191 4_2:-63943595 None:intergenic
TTCATCGAGGGCCGGTCATT+GGG 0.350816 4_2:-63944840 None:intergenic
TGAAGTCTTGTCCATCGGTT+CGG 0.370019 4_2:-63944501 None:intergenic
GAAGTCTTGTCCATCGGTTC+GGG 0.377519 4_2:-63944500 None:intergenic
GTGGATCAGGTGGAAGGTAT+TGG 0.388395 4_2:-63944899 None:intergenic
AGAAAAGTGACTGCTGCATC+GGG 0.420562 4_2:-63943984 None:intergenic
AGAGAATGGAAATTGATGAT+AGG 0.442030 4_2:-63943544 None:intergenic
ATGGATTTATGATCAATTTG+AGG 0.446936 4_2:+63944728 Msa0614810:CDS
ATATTCTTCATGATTAGGTT+CGG 0.448191 4_2:-63943590 None:intergenic
TGATGATGAGGAGGAGGAGG+AGG 0.451275 4_2:-63943473 None:intergenic
ATCAACGAAGTTGCAACCAT+TGG 0.461884 4_2:+63944523 Msa0614810:CDS
ATGATAGGACAGTTATTCTC+AGG 0.487448 4_2:-63943529 None:intergenic
GAAGTGATAGAAATGCTTGA+AGG 0.493798 4_2:+63944864 Msa0614810:CDS
CTGTCTTGCTCACAGCGTAA+TGG 0.495003 4_2:-63943901 None:intergenic
GTTAACTGAGATTGTGGATC+AGG 0.495405 4_2:-63944912 None:intergenic
ACAGAGACAAAGACGGAGAC+TGG 0.497209 4_2:+63943920 Msa0614810:CDS
TATATTGTCGCGACTGCTGA+CGG 0.499007 4_2:+63943721 Msa0614810:CDS
GTTCACAGTGAGTACGTGAA+TGG 0.508012 4_2:+63943859 Msa0614810:CDS
TGCCATGTTAACTGAGATTG+TGG 0.513788 4_2:-63944918 None:intergenic
TTCATGATTAGGTTCGGTAA+AGG 0.515056 4_2:-63943584 None:intergenic
TACAAATGAAGCCCAATGAC+CGG 0.518243 4_2:+63944829 Msa0614810:CDS
AAGAAAAGTGACTGCTGCAT+CGG 0.519844 4_2:-63943985 None:intergenic
TCAAAATGGCTATGTCGTGC+GGG 0.520488 4_2:+63943209 None:intergenic
TGAATGGAATTGAGTTTCGT+TGG 0.522029 4_2:+63943875 Msa0614810:CDS
GTTGATGAAGTCTTGTCCAT+CGG 0.526164 4_2:-63944506 None:intergenic
CGCTAATCATTGATGAGTGT+TGG 0.536570 4_2:-63944977 None:intergenic
ATGAACTCGTAGACGAGAGC+TGG 0.537554 4_2:-63944604 None:intergenic
TAACTCCGGTGATTATGTAA+TGG 0.543618 4_2:-63943504 None:intergenic
TGATGATGATGAGGAGGAGG+AGG 0.545572 4_2:-63943476 None:intergenic
TTTCGCCGCCTTTGTAACAC+TGG 0.549294 4_2:+63943243 Msa0614810:CDS
GTCATGCAGAATAGAGATGA+TGG 0.554727 4_2:+63943768 Msa0614810:CDS
GACATTAATTCTGAGAGTTG+TGG 0.555244 4_2:+63943661 Msa0614810:CDS
GACGGAGACTGGTATGGCGA+AGG 0.559547 4_2:+63943931 Msa0614810:CDS
ATCAAAATGGCTATGTCGTG+CGG 0.568132 4_2:+63943208 None:intergenic
GACATGACGAATTCTTCCAA+TGG 0.572994 4_2:-63944539 None:intergenic
GGATCAGGTGGAAGGTATTG+GGG 0.573681 4_2:-63944897 None:intergenic
ATTAATTCTGAGAGTTGTGG+TGG 0.575431 4_2:+63943664 Msa0614810:CDS
GACAAAGACGGAGACTGGTA+TGG 0.578629 4_2:+63943925 Msa0614810:CDS
TGGTGGAAGAATTGACGTGA+AGG 0.580282 4_2:+63943681 Msa0614810:CDS
CACACATACCAGTGTTACAA+AGG 0.585510 4_2:-63943251 None:intergenic
TCATTCCATTACATAATCAC+CGG 0.586235 4_2:+63943499 Msa0614810:CDS
TCTATCACTTCATTCATCGA+GGG 0.589618 4_2:-63944852 None:intergenic
TCACTTCATTCATCGAGGGC+CGG 0.589948 4_2:-63944848 None:intergenic
TGGCAAAGGATGTCAACAGA+TGG 0.590410 4_2:+63944936 Msa0614810:CDS
TTGACCAAGTCCCGAACCGA+TGG 0.597161 4_2:+63944490 Msa0614810:intron
CTGCATCGGGGAGAGTGCAG+CGG 0.597190 4_2:-63943971 None:intergenic
GAGATTGTGGATCAGGTGGA+AGG 0.605232 4_2:-63944905 None:intergenic
TTCTATCACTTCATTCATCG+AGG 0.606497 4_2:-63944853 None:intergenic
ATCCACAATCTCAGTTAACA+TGG 0.618011 4_2:+63944916 Msa0614810:CDS
GTCTACGAGTTCATGCCCAA+TGG 0.618308 4_2:+63944613 Msa0614810:CDS
CAAAATGGCTATGTCGTGCG+GGG 0.621169 4_2:+63943210 None:intergenic
AACTGAGATTGTGGATCAGG+TGG 0.621802 4_2:-63944909 None:intergenic
TGCAAACAATGTAACAGAGG+AGG 0.622960 4_2:+63944761 Msa0614810:CDS
ACGGAGACTGGTATGGCGAA+GGG 0.623518 4_2:+63943932 Msa0614810:CDS
CTCATCAATGATTAGCGGAG+TGG 0.627380 4_2:+63944983 Msa0614810:CDS
CAACACTCATCAATGATTAG+CGG 0.633054 4_2:+63944978 Msa0614810:CDS
GAGCAAGACAGAGACAAAGA+CGG 0.635443 4_2:+63943913 Msa0614810:CDS
AAAATGGCTATGTCGTGCGG+GGG 0.643949 4_2:+63943211 None:intergenic
TCGGGGAGAGTGCAGCGGAG+TGG 0.652591 4_2:-63943966 None:intergenic
AATCTCAGTTAACATGGCAA+AGG 0.652700 4_2:+63944922 Msa0614810:CDS
TAATCATGAAGAATATATGG+TGG 0.658942 4_2:+63943597 Msa0614810:CDS
AAATGGCTATGTCGTGCGGG+GGG 0.661728 4_2:+63943212 None:intergenic
AGATGCAAACAATGTAACAG+AGG 0.665956 4_2:+63944758 Msa0614810:CDS
GGTGGAAGAATTGACGTGAA+GGG 0.680896 4_2:+63943682 Msa0614810:CDS
GGAGGAATTAGAAGCTACAA+CGG 0.692315 4_2:-63943455 None:intergenic
GTTATTCTCAGGACTAACTC+CGG 0.693362 4_2:-63943518 None:intergenic
ACATACCAGTGTTACAAAGG+CGG 0.694014 4_2:-63943248 None:intergenic
GAAAAGTGACTGCTGCATCG+GGG 0.726762 4_2:-63943983 None:intergenic
AAGAATTCGTCATGTCAATG+TGG 0.774637 4_2:+63944546 Msa0614810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTGTTTTTATTTACTTTTC+CGG + chr4_2:63943270-63943289 Msa0614810:intron 20.0%
!!! AAACATTTCAAGTATAAGTT+GGG + chr4_2:63944400-63944419 Msa0614810:intron 20.0%
!!! TGTGTTTTTATTTACTTTTC+CGG + chr4_2:63943270-63943289 Msa0614810:intron 20.0%
!!! AAACATTTCAAGTATAAGTT+GGG + chr4_2:63944400-63944419 Msa0614810:intron 20.0%
! AACAAATCTTCATACTCATT+TGG + chr4_2:63943343-63943362 Msa0614810:intron 25.0%
! AATATGCAAAAACAACAACT+CGG + chr4_2:63943396-63943415 Msa0614810:intron 25.0%
! ATATTCTTCATGATTAGGTT+CGG - chr4_2:63943593-63943612 None:intergenic 25.0%
! ACCTAATCATGAAGAATATA+TGG + chr4_2:63943594-63943613 Msa0614810:CDS 25.0%
! ACCATATATTCTTCATGATT+AGG - chr4_2:63943598-63943617 None:intergenic 25.0%
! TAATCATGAAGAATATATGG+TGG + chr4_2:63943597-63943616 Msa0614810:CDS 25.0%
! AAAAAACTGGTAGATCATTA+CGG + chr4_2:63943631-63943650 Msa0614810:CDS 25.0%
! AAAAAGAATGCCTAAACTAT+GGG - chr4_2:63944082-63944101 None:intergenic 25.0%
! AAAAAAGAATGCCTAAACTA+TGG - chr4_2:63944083-63944102 None:intergenic 25.0%
! GCATTTATCTATATATGACA+CGG + chr4_2:63944303-63944322 Msa0614810:intron 25.0%
!! GCATGAAATTATTGTTACTT+TGG - chr4_2:63944342-63944361 None:intergenic 25.0%
! CAACTTATACTTGAAATGTT+TGG - chr4_2:63944401-63944420 None:intergenic 25.0%
!! CAAACATTTCAAGTATAAGT+TGG + chr4_2:63944399-63944418 Msa0614810:intron 25.0%
! ATGGATTTATGATCAATTTG+AGG + chr4_2:63944728-63944747 Msa0614810:CDS 25.0%
! AACAAATCTTCATACTCATT+TGG + chr4_2:63943343-63943362 Msa0614810:intron 25.0%
! AATATGCAAAAACAACAACT+CGG + chr4_2:63943396-63943415 Msa0614810:intron 25.0%
! ATATTCTTCATGATTAGGTT+CGG - chr4_2:63943593-63943612 None:intergenic 25.0%
! ACCTAATCATGAAGAATATA+TGG + chr4_2:63943594-63943613 Msa0614810:CDS 25.0%
! ACCATATATTCTTCATGATT+AGG - chr4_2:63943598-63943617 None:intergenic 25.0%
! TAATCATGAAGAATATATGG+TGG + chr4_2:63943597-63943616 Msa0614810:CDS 25.0%
! AAAAAACTGGTAGATCATTA+CGG + chr4_2:63943631-63943650 Msa0614810:CDS 25.0%
! AAAAAGAATGCCTAAACTAT+GGG - chr4_2:63944082-63944101 None:intergenic 25.0%
! AAAAAAGAATGCCTAAACTA+TGG - chr4_2:63944083-63944102 None:intergenic 25.0%
! GCATTTATCTATATATGACA+CGG + chr4_2:63944303-63944322 Msa0614810:intron 25.0%
!! GCATGAAATTATTGTTACTT+TGG - chr4_2:63944342-63944361 None:intergenic 25.0%
! CAACTTATACTTGAAATGTT+TGG - chr4_2:63944401-63944420 None:intergenic 25.0%
!! CAAACATTTCAAGTATAAGT+TGG + chr4_2:63944399-63944418 Msa0614810:intron 25.0%
! ATGGATTTATGATCAATTTG+AGG + chr4_2:63944728-63944747 Msa0614810:CDS 25.0%
AAGGTTGTTCTACTAAAATG+GGG - chr4_2:63943309-63943328 None:intergenic 30.0%
CAAGGTTGTTCTACTAAAAT+GGG - chr4_2:63943310-63943329 None:intergenic 30.0%
ACAAGGTTGTTCTACTAAAA+TGG - chr4_2:63943311-63943330 None:intergenic 30.0%
!! ATTTTAGTAGAACAACCTTG+TGG + chr4_2:63943310-63943329 Msa0614810:intron 30.0%
TTGTTGAATATGATTCCACA+AGG - chr4_2:63943328-63943347 None:intergenic 30.0%
TCGGTAATATACTTCAACTA+TGG + chr4_2:63943415-63943434 Msa0614810:intron 30.0%
TCATTCCATTACATAATCAC+CGG + chr4_2:63943499-63943518 Msa0614810:CDS 30.0%
! AGAGAATGGAAATTGATGAT+AGG - chr4_2:63943547-63943566 None:intergenic 30.0%
AAAAACTGGTAGATCATTAC+GGG + chr4_2:63943632-63943651 Msa0614810:CDS 30.0%
TATGTTGGAAAATGTGTTCT+TGG + chr4_2:63944235-63944254 Msa0614810:intron 30.0%
ATGTTGGAAAATGTGTTCTT+GGG + chr4_2:63944236-63944255 Msa0614810:intron 30.0%
TTGCTGTCTTGATATGTAAA+TGG + chr4_2:63944272-63944291 Msa0614810:intron 30.0%
TGTTTATCAAGAGAACCATT+GGG - chr4_2:63944631-63944650 None:intergenic 30.0%
CACAAGATTACTTTGCTAAA+TGG + chr4_2:63944709-63944728 Msa0614810:intron 30.0%
AAGGTTGTTCTACTAAAATG+GGG - chr4_2:63943309-63943328 None:intergenic 30.0%
CAAGGTTGTTCTACTAAAAT+GGG - chr4_2:63943310-63943329 None:intergenic 30.0%
ACAAGGTTGTTCTACTAAAA+TGG - chr4_2:63943311-63943330 None:intergenic 30.0%
!! ATTTTAGTAGAACAACCTTG+TGG + chr4_2:63943310-63943329 Msa0614810:intron 30.0%
TTGTTGAATATGATTCCACA+AGG - chr4_2:63943328-63943347 None:intergenic 30.0%
TCGGTAATATACTTCAACTA+TGG + chr4_2:63943415-63943434 Msa0614810:intron 30.0%
TCATTCCATTACATAATCAC+CGG + chr4_2:63943499-63943518 Msa0614810:CDS 30.0%
! AGAGAATGGAAATTGATGAT+AGG - chr4_2:63943547-63943566 None:intergenic 30.0%
AAAAACTGGTAGATCATTAC+GGG + chr4_2:63943632-63943651 Msa0614810:CDS 30.0%
TATGTTGGAAAATGTGTTCT+TGG + chr4_2:63944235-63944254 Msa0614810:intron 30.0%
ATGTTGGAAAATGTGTTCTT+GGG + chr4_2:63944236-63944255 Msa0614810:intron 30.0%
TTGCTGTCTTGATATGTAAA+TGG + chr4_2:63944272-63944291 Msa0614810:intron 30.0%
TGTTTATCAAGAGAACCATT+GGG - chr4_2:63944631-63944650 None:intergenic 30.0%
CACAAGATTACTTTGCTAAA+TGG + chr4_2:63944709-63944728 Msa0614810:intron 30.0%
ATCTTCATACTCATTTGGAC+AGG + chr4_2:63943348-63943367 Msa0614810:intron 35.0%
TCTTCATACTCATTTGGACA+GGG + chr4_2:63943349-63943368 Msa0614810:intron 35.0%
! ATGGAATGATGATGATGATG+AGG - chr4_2:63943488-63943507 None:intergenic 35.0%
TAACTCCGGTGATTATGTAA+TGG - chr4_2:63943507-63943526 None:intergenic 35.0%
ATGATAGGACAGTTATTCTC+AGG - chr4_2:63943532-63943551 None:intergenic 35.0%
! TTCATGATTAGGTTCGGTAA+AGG - chr4_2:63943587-63943606 None:intergenic 35.0%
! GACATTAATTCTGAGAGTTG+TGG + chr4_2:63943661-63943680 Msa0614810:CDS 35.0%
! ATTAATTCTGAGAGTTGTGG+TGG + chr4_2:63943664-63943683 Msa0614810:CDS 35.0%
! CAGAATAGAGATGATGGTAA+TGG + chr4_2:63943774-63943793 Msa0614810:intron 35.0%
TGAATGGAATTGAGTTTCGT+TGG + chr4_2:63943875-63943894 Msa0614810:CDS 35.0%
GTAAGTCAGTTGTAAAACAG+TGG - chr4_2:63944116-63944135 None:intergenic 35.0%
! ATTTCTTTTCAGTGATCCCA+TGG + chr4_2:63944212-63944231 Msa0614810:intron 35.0%
! CACATTTTCCAACATACCAT+GGG - chr4_2:63944231-63944250 None:intergenic 35.0%
! ACACATTTTCCAACATACCA+TGG - chr4_2:63944232-63944251 None:intergenic 35.0%
ATCAAGACAGCAATCACAAA+GGG - chr4_2:63944265-63944284 None:intergenic 35.0%
TATCAAGACAGCAATCACAA+AGG - chr4_2:63944266-63944285 None:intergenic 35.0%
!! TGAGAGTAAGAGTATCTGAT+GGG - chr4_2:63944364-63944383 None:intergenic 35.0%
!! TTGAGAGTAAGAGTATCTGA+TGG - chr4_2:63944365-63944384 None:intergenic 35.0%
! TTCAAGTATAAGTTGGGTGA+AGG + chr4_2:63944406-63944425 Msa0614810:intron 35.0%
AAGAATTCGTCATGTCAATG+TGG + chr4_2:63944546-63944565 Msa0614810:CDS 35.0%
GTCAATGTGGTGCAATTAAT+TGG + chr4_2:63944559-63944578 Msa0614810:CDS 35.0%
GTGTTTATCAAGAGAACCAT+TGG - chr4_2:63944632-63944651 None:intergenic 35.0%
! TAAACACACGTTTTCACAAG+AGG + chr4_2:63944645-63944664 Msa0614810:intron 35.0%
AGATGCAAACAATGTAACAG+AGG + chr4_2:63944758-63944777 Msa0614810:CDS 35.0%
TCTATCACTTCATTCATCGA+GGG - chr4_2:63944855-63944874 None:intergenic 35.0%
TTCTATCACTTCATTCATCG+AGG - chr4_2:63944856-63944875 None:intergenic 35.0%
GAAGTGATAGAAATGCTTGA+AGG + chr4_2:63944864-63944883 Msa0614810:CDS 35.0%
ATCCACAATCTCAGTTAACA+TGG + chr4_2:63944916-63944935 Msa0614810:CDS 35.0%
AATCTCAGTTAACATGGCAA+AGG + chr4_2:63944922-63944941 Msa0614810:CDS 35.0%
CAACACTCATCAATGATTAG+CGG + chr4_2:63944978-63944997 Msa0614810:CDS 35.0%
ATCTTCATACTCATTTGGAC+AGG + chr4_2:63943348-63943367 Msa0614810:intron 35.0%
TCTTCATACTCATTTGGACA+GGG + chr4_2:63943349-63943368 Msa0614810:intron 35.0%
! ATGGAATGATGATGATGATG+AGG - chr4_2:63943488-63943507 None:intergenic 35.0%
TAACTCCGGTGATTATGTAA+TGG - chr4_2:63943507-63943526 None:intergenic 35.0%
ATGATAGGACAGTTATTCTC+AGG - chr4_2:63943532-63943551 None:intergenic 35.0%
! TTCATGATTAGGTTCGGTAA+AGG - chr4_2:63943587-63943606 None:intergenic 35.0%
! GACATTAATTCTGAGAGTTG+TGG + chr4_2:63943661-63943680 Msa0614810:CDS 35.0%
! ATTAATTCTGAGAGTTGTGG+TGG + chr4_2:63943664-63943683 Msa0614810:CDS 35.0%
! CAGAATAGAGATGATGGTAA+TGG + chr4_2:63943774-63943793 Msa0614810:intron 35.0%
TGAATGGAATTGAGTTTCGT+TGG + chr4_2:63943875-63943894 Msa0614810:CDS 35.0%
GTAAGTCAGTTGTAAAACAG+TGG - chr4_2:63944116-63944135 None:intergenic 35.0%
! ATTTCTTTTCAGTGATCCCA+TGG + chr4_2:63944212-63944231 Msa0614810:intron 35.0%
! CACATTTTCCAACATACCAT+GGG - chr4_2:63944231-63944250 None:intergenic 35.0%
! ACACATTTTCCAACATACCA+TGG - chr4_2:63944232-63944251 None:intergenic 35.0%
ATCAAGACAGCAATCACAAA+GGG - chr4_2:63944265-63944284 None:intergenic 35.0%
TATCAAGACAGCAATCACAA+AGG - chr4_2:63944266-63944285 None:intergenic 35.0%
!! TGAGAGTAAGAGTATCTGAT+GGG - chr4_2:63944364-63944383 None:intergenic 35.0%
!! TTGAGAGTAAGAGTATCTGA+TGG - chr4_2:63944365-63944384 None:intergenic 35.0%
! TTCAAGTATAAGTTGGGTGA+AGG + chr4_2:63944406-63944425 Msa0614810:intron 35.0%
AAGAATTCGTCATGTCAATG+TGG + chr4_2:63944546-63944565 Msa0614810:CDS 35.0%
GTCAATGTGGTGCAATTAAT+TGG + chr4_2:63944559-63944578 Msa0614810:CDS 35.0%
GTGTTTATCAAGAGAACCAT+TGG - chr4_2:63944632-63944651 None:intergenic 35.0%
! TAAACACACGTTTTCACAAG+AGG + chr4_2:63944645-63944664 Msa0614810:intron 35.0%
AGATGCAAACAATGTAACAG+AGG + chr4_2:63944758-63944777 Msa0614810:CDS 35.0%
TCTATCACTTCATTCATCGA+GGG - chr4_2:63944855-63944874 None:intergenic 35.0%
TTCTATCACTTCATTCATCG+AGG - chr4_2:63944856-63944875 None:intergenic 35.0%
GAAGTGATAGAAATGCTTGA+AGG + chr4_2:63944864-63944883 Msa0614810:CDS 35.0%
ATCCACAATCTCAGTTAACA+TGG + chr4_2:63944916-63944935 Msa0614810:CDS 35.0%
AATCTCAGTTAACATGGCAA+AGG + chr4_2:63944922-63944941 Msa0614810:CDS 35.0%
CAACACTCATCAATGATTAG+CGG + chr4_2:63944978-63944997 Msa0614810:CDS 35.0%
ACATACCAGTGTTACAAAGG+CGG - chr4_2:63943251-63943270 None:intergenic 40.0%
CACACATACCAGTGTTACAA+AGG - chr4_2:63943254-63943273 None:intergenic 40.0%
CTTCATACTCATTTGGACAG+GGG + chr4_2:63943350-63943369 Msa0614810:intron 40.0%
GGAGGAATTAGAAGCTACAA+CGG - chr4_2:63943458-63943477 None:intergenic 40.0%
! GAATGATGATGATGATGAGG+AGG - chr4_2:63943485-63943504 None:intergenic 40.0%
GTTATTCTCAGGACTAACTC+CGG - chr4_2:63943521-63943540 None:intergenic 40.0%
! CGTTTTCGTATGGAAGAGAA+TGG - chr4_2:63943561-63943580 None:intergenic 40.0%
! GTAAAGGAGACGTTTTCGTA+TGG - chr4_2:63943571-63943590 None:intergenic 40.0%
GGTTGTGAGATGCAAAAAAC+TGG + chr4_2:63943618-63943637 Msa0614810:CDS 40.0%
GTCATGCAGAATAGAGATGA+TGG + chr4_2:63943768-63943787 Msa0614810:CDS 40.0%
CTTTCCATATTTCCACCTAC+TGG - chr4_2:63943800-63943819 None:intergenic 40.0%
CAGTAGGTGGAAATATGGAA+AGG + chr4_2:63943798-63943817 Msa0614810:intron 40.0%
AAGCAAGTGAGAATATCGCA+AGG + chr4_2:63943837-63943856 Msa0614810:intron 40.0%
AAGAAAAGTGACTGCTGCAT+CGG - chr4_2:63943988-63944007 None:intergenic 40.0%
!!! GCAGTCACTTTTCTTGCTAT+CGG + chr4_2:63943993-63944012 Msa0614810:CDS 40.0%
AATGCCTAAACTATGGGTGA+GGG - chr4_2:63944076-63944095 None:intergenic 40.0%
! AAGTATAAGTTGGGTGAAGG+AGG + chr4_2:63944409-63944428 Msa0614810:intron 40.0%
! GTTGATGAAGTCTTGTCCAT+CGG - chr4_2:63944509-63944528 None:intergenic 40.0%
ATCAACGAAGTTGCAACCAT+TGG + chr4_2:63944523-63944542 Msa0614810:CDS 40.0%
GACATGACGAATTCTTCCAA+TGG - chr4_2:63944542-63944561 None:intergenic 40.0%
TGCAAACAATGTAACAGAGG+AGG + chr4_2:63944761-63944780 Msa0614810:CDS 40.0%
TACAAATGAAGCCCAATGAC+CGG + chr4_2:63944829-63944848 Msa0614810:CDS 40.0%
GTTAACTGAGATTGTGGATC+AGG - chr4_2:63944915-63944934 None:intergenic 40.0%
TGCCATGTTAACTGAGATTG+TGG - chr4_2:63944921-63944940 None:intergenic 40.0%
CGCTAATCATTGATGAGTGT+TGG - chr4_2:63944980-63944999 None:intergenic 40.0%
ACATACCAGTGTTACAAAGG+CGG - chr4_2:63943251-63943270 None:intergenic 40.0%
CACACATACCAGTGTTACAA+AGG - chr4_2:63943254-63943273 None:intergenic 40.0%
CTTCATACTCATTTGGACAG+GGG + chr4_2:63943350-63943369 Msa0614810:intron 40.0%
GGAGGAATTAGAAGCTACAA+CGG - chr4_2:63943458-63943477 None:intergenic 40.0%
! GAATGATGATGATGATGAGG+AGG - chr4_2:63943485-63943504 None:intergenic 40.0%
GTTATTCTCAGGACTAACTC+CGG - chr4_2:63943521-63943540 None:intergenic 40.0%
! CGTTTTCGTATGGAAGAGAA+TGG - chr4_2:63943561-63943580 None:intergenic 40.0%
! GTAAAGGAGACGTTTTCGTA+TGG - chr4_2:63943571-63943590 None:intergenic 40.0%
GGTTGTGAGATGCAAAAAAC+TGG + chr4_2:63943618-63943637 Msa0614810:CDS 40.0%
GTCATGCAGAATAGAGATGA+TGG + chr4_2:63943768-63943787 Msa0614810:CDS 40.0%
CTTTCCATATTTCCACCTAC+TGG - chr4_2:63943800-63943819 None:intergenic 40.0%
CAGTAGGTGGAAATATGGAA+AGG + chr4_2:63943798-63943817 Msa0614810:intron 40.0%
AAGCAAGTGAGAATATCGCA+AGG + chr4_2:63943837-63943856 Msa0614810:intron 40.0%
AAGAAAAGTGACTGCTGCAT+CGG - chr4_2:63943988-63944007 None:intergenic 40.0%
!!! GCAGTCACTTTTCTTGCTAT+CGG + chr4_2:63943993-63944012 Msa0614810:CDS 40.0%
AATGCCTAAACTATGGGTGA+GGG - chr4_2:63944076-63944095 None:intergenic 40.0%
! AAGTATAAGTTGGGTGAAGG+AGG + chr4_2:63944409-63944428 Msa0614810:intron 40.0%
! GTTGATGAAGTCTTGTCCAT+CGG - chr4_2:63944509-63944528 None:intergenic 40.0%
ATCAACGAAGTTGCAACCAT+TGG + chr4_2:63944523-63944542 Msa0614810:CDS 40.0%
GACATGACGAATTCTTCCAA+TGG - chr4_2:63944542-63944561 None:intergenic 40.0%
TGCAAACAATGTAACAGAGG+AGG + chr4_2:63944761-63944780 Msa0614810:CDS 40.0%
TACAAATGAAGCCCAATGAC+CGG + chr4_2:63944829-63944848 Msa0614810:CDS 40.0%
GTTAACTGAGATTGTGGATC+AGG - chr4_2:63944915-63944934 None:intergenic 40.0%
TGCCATGTTAACTGAGATTG+TGG - chr4_2:63944921-63944940 None:intergenic 40.0%
CGCTAATCATTGATGAGTGT+TGG - chr4_2:63944980-63944999 None:intergenic 40.0%
! TGATGATGATGATGAGGAGG+AGG - chr4_2:63943482-63943501 None:intergenic 45.0%
TGGTGGAAGAATTGACGTGA+AGG + chr4_2:63943681-63943700 Msa0614810:CDS 45.0%
GGTGGAAGAATTGACGTGAA+GGG + chr4_2:63943682-63943701 Msa0614810:CDS 45.0%
TATATTGTCGCGACTGCTGA+CGG + chr4_2:63943721-63943740 Msa0614810:CDS 45.0%
!! AGATGATGGTAATGGCCAGT+AGG + chr4_2:63943782-63943801 Msa0614810:intron 45.0%
ATGGCCAGTAGGTGGAAATA+TGG + chr4_2:63943793-63943812 Msa0614810:intron 45.0%
GTTCACAGTGAGTACGTGAA+TGG + chr4_2:63943859-63943878 Msa0614810:CDS 45.0%
GAGCAAGACAGAGACAAAGA+CGG + chr4_2:63943913-63943932 Msa0614810:CDS 45.0%
AGAAAAGTGACTGCTGCATC+GGG - chr4_2:63943987-63944006 None:intergenic 45.0%
TACTCCCTCACCCATAGTTT+AGG + chr4_2:63944069-63944088 Msa0614810:intron 45.0%
GAATGCCTAAACTATGGGTG+AGG - chr4_2:63944077-63944096 None:intergenic 45.0%
TCAGTGATCCCATGGTATGT+TGG + chr4_2:63944220-63944239 Msa0614810:intron 45.0%
! TTTACGAAGGAGAGCTCTGA+AGG + chr4_2:63944443-63944462 Msa0614810:intron 45.0%
! TGAAGTCTTGTCCATCGGTT+CGG - chr4_2:63944504-63944523 None:intergenic 45.0%
! CACACGTTTTCACAAGAGGA+AGG + chr4_2:63944649-63944668 Msa0614810:intron 45.0%
!! TGGATCAGGTGGAAGGTATT+GGG - chr4_2:63944901-63944920 None:intergenic 45.0%
AACTGAGATTGTGGATCAGG+TGG - chr4_2:63944912-63944931 None:intergenic 45.0%
TGGCAAAGGATGTCAACAGA+TGG + chr4_2:63944936-63944955 Msa0614810:CDS 45.0%
CTCATCAATGATTAGCGGAG+TGG + chr4_2:63944983-63945002 Msa0614810:CDS 45.0%
! TGATGATGATGATGAGGAGG+AGG - chr4_2:63943482-63943501 None:intergenic 45.0%
TGGTGGAAGAATTGACGTGA+AGG + chr4_2:63943681-63943700 Msa0614810:CDS 45.0%
GGTGGAAGAATTGACGTGAA+GGG + chr4_2:63943682-63943701 Msa0614810:CDS 45.0%
TATATTGTCGCGACTGCTGA+CGG + chr4_2:63943721-63943740 Msa0614810:CDS 45.0%
!! AGATGATGGTAATGGCCAGT+AGG + chr4_2:63943782-63943801 Msa0614810:intron 45.0%
ATGGCCAGTAGGTGGAAATA+TGG + chr4_2:63943793-63943812 Msa0614810:intron 45.0%
GTTCACAGTGAGTACGTGAA+TGG + chr4_2:63943859-63943878 Msa0614810:CDS 45.0%
GAGCAAGACAGAGACAAAGA+CGG + chr4_2:63943913-63943932 Msa0614810:CDS 45.0%
AGAAAAGTGACTGCTGCATC+GGG - chr4_2:63943987-63944006 None:intergenic 45.0%
TACTCCCTCACCCATAGTTT+AGG + chr4_2:63944069-63944088 Msa0614810:intron 45.0%
GAATGCCTAAACTATGGGTG+AGG - chr4_2:63944077-63944096 None:intergenic 45.0%
TCAGTGATCCCATGGTATGT+TGG + chr4_2:63944220-63944239 Msa0614810:intron 45.0%
! TTTACGAAGGAGAGCTCTGA+AGG + chr4_2:63944443-63944462 Msa0614810:intron 45.0%
! TGAAGTCTTGTCCATCGGTT+CGG - chr4_2:63944504-63944523 None:intergenic 45.0%
! CACACGTTTTCACAAGAGGA+AGG + chr4_2:63944649-63944668 Msa0614810:intron 45.0%
!! TGGATCAGGTGGAAGGTATT+GGG - chr4_2:63944901-63944920 None:intergenic 45.0%
AACTGAGATTGTGGATCAGG+TGG - chr4_2:63944912-63944931 None:intergenic 45.0%
TGGCAAAGGATGTCAACAGA+TGG + chr4_2:63944936-63944955 Msa0614810:CDS 45.0%
CTCATCAATGATTAGCGGAG+TGG + chr4_2:63944983-63945002 Msa0614810:CDS 45.0%
TTTCGCCGCCTTTGTAACAC+TGG + chr4_2:63943243-63943262 Msa0614810:CDS 50.0%
ATGGGGCATAAAACACGTGC+CGG - chr4_2:63943292-63943311 None:intergenic 50.0%
! TGATGATGATGAGGAGGAGG+AGG - chr4_2:63943479-63943498 None:intergenic 50.0%
!! TGATGGTAATGGCCAGTAGG+TGG + chr4_2:63943785-63943804 Msa0614810:intron 50.0%
CTGTCTTGCTCACAGCGTAA+TGG - chr4_2:63943904-63943923 None:intergenic 50.0%
ACAGAGACAAAGACGGAGAC+TGG + chr4_2:63943920-63943939 Msa0614810:CDS 50.0%
GACAAAGACGGAGACTGGTA+TGG + chr4_2:63943925-63943944 Msa0614810:CDS 50.0%
GAAAAGTGACTGCTGCATCG+GGG - chr4_2:63943986-63944005 None:intergenic 50.0%
CTATGGGTGAGGGAGTAACT+TGG - chr4_2:63944066-63944085 None:intergenic 50.0%
AAGTTGGGTGAAGGAGGCTA+CGG + chr4_2:63944415-63944434 Msa0614810:intron 50.0%
GGCTACGGTTCTGTTTACGA+AGG + chr4_2:63944430-63944449 Msa0614810:intron 50.0%
!! GAAGTCTTGTCCATCGGTTC+GGG - chr4_2:63944503-63944522 None:intergenic 50.0%
ATGAACTCGTAGACGAGAGC+TGG - chr4_2:63944607-63944626 None:intergenic 50.0%
GTCTACGAGTTCATGCCCAA+TGG + chr4_2:63944613-63944632 Msa0614810:CDS 50.0%
TCACTTCATTCATCGAGGGC+CGG - chr4_2:63944851-63944870 None:intergenic 50.0%
!! GGATCAGGTGGAAGGTATTG+GGG - chr4_2:63944900-63944919 None:intergenic 50.0%
! GTGGATCAGGTGGAAGGTAT+TGG - chr4_2:63944902-63944921 None:intergenic 50.0%
GAGATTGTGGATCAGGTGGA+AGG - chr4_2:63944908-63944927 None:intergenic 50.0%
TTTCGCCGCCTTTGTAACAC+TGG + chr4_2:63943243-63943262 Msa0614810:CDS 50.0%
ATGGGGCATAAAACACGTGC+CGG - chr4_2:63943292-63943311 None:intergenic 50.0%
! TGATGATGATGAGGAGGAGG+AGG - chr4_2:63943479-63943498 None:intergenic 50.0%
!! TGATGGTAATGGCCAGTAGG+TGG + chr4_2:63943785-63943804 Msa0614810:intron 50.0%
CTGTCTTGCTCACAGCGTAA+TGG - chr4_2:63943904-63943923 None:intergenic 50.0%
ACAGAGACAAAGACGGAGAC+TGG + chr4_2:63943920-63943939 Msa0614810:CDS 50.0%
GACAAAGACGGAGACTGGTA+TGG + chr4_2:63943925-63943944 Msa0614810:CDS 50.0%
GAAAAGTGACTGCTGCATCG+GGG - chr4_2:63943986-63944005 None:intergenic 50.0%
CTATGGGTGAGGGAGTAACT+TGG - chr4_2:63944066-63944085 None:intergenic 50.0%
AAGTTGGGTGAAGGAGGCTA+CGG + chr4_2:63944415-63944434 Msa0614810:intron 50.0%
GGCTACGGTTCTGTTTACGA+AGG + chr4_2:63944430-63944449 Msa0614810:intron 50.0%
!! GAAGTCTTGTCCATCGGTTC+GGG - chr4_2:63944503-63944522 None:intergenic 50.0%
ATGAACTCGTAGACGAGAGC+TGG - chr4_2:63944607-63944626 None:intergenic 50.0%
GTCTACGAGTTCATGCCCAA+TGG + chr4_2:63944613-63944632 Msa0614810:CDS 50.0%
TCACTTCATTCATCGAGGGC+CGG - chr4_2:63944851-63944870 None:intergenic 50.0%
!! GGATCAGGTGGAAGGTATTG+GGG - chr4_2:63944900-63944919 None:intergenic 50.0%
! GTGGATCAGGTGGAAGGTAT+TGG - chr4_2:63944902-63944921 None:intergenic 50.0%
GAGATTGTGGATCAGGTGGA+AGG - chr4_2:63944908-63944927 None:intergenic 50.0%
! TGATGATGAGGAGGAGGAGG+AGG - chr4_2:63943476-63943495 None:intergenic 55.0%
ACGGAGACTGGTATGGCGAA+GGG + chr4_2:63943932-63943951 Msa0614810:CDS 55.0%
TTGACCAAGTCCCGAACCGA+TGG + chr4_2:63944490-63944509 Msa0614810:intron 55.0%
!! TTGTCCATCGGTTCGGGACT+TGG - chr4_2:63944497-63944516 None:intergenic 55.0%
TTCATCGAGGGCCGGTCATT+GGG - chr4_2:63944843-63944862 None:intergenic 55.0%
ATTCATCGAGGGCCGGTCAT+TGG - chr4_2:63944844-63944863 None:intergenic 55.0%
! TGATGATGAGGAGGAGGAGG+AGG - chr4_2:63943476-63943495 None:intergenic 55.0%
ACGGAGACTGGTATGGCGAA+GGG + chr4_2:63943932-63943951 Msa0614810:CDS 55.0%
TTGACCAAGTCCCGAACCGA+TGG + chr4_2:63944490-63944509 Msa0614810:intron 55.0%
!! TTGTCCATCGGTTCGGGACT+TGG - chr4_2:63944497-63944516 None:intergenic 55.0%
TTCATCGAGGGCCGGTCATT+GGG - chr4_2:63944843-63944862 None:intergenic 55.0%
ATTCATCGAGGGCCGGTCAT+TGG - chr4_2:63944844-63944863 None:intergenic 55.0%
GACGGAGACTGGTATGGCGA+AGG + chr4_2:63943931-63943950 Msa0614810:CDS 60.0%
GACGGAGACTGGTATGGCGA+AGG + chr4_2:63943931-63943950 Msa0614810:CDS 60.0%
! CTGCATCGGGGAGAGTGCAG+CGG - chr4_2:63943974-63943993 None:intergenic 65.0%
! CTGCATCGGGGAGAGTGCAG+CGG - chr4_2:63943974-63943993 None:intergenic 65.0%
! TCGGGGAGAGTGCAGCGGAG+TGG - chr4_2:63943969-63943988 None:intergenic 70.0%
! TCGGGGAGAGTGCAGCGGAG+TGG - chr4_2:63943969-63943988 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 63943214 63945022 63943214 ID=Msa0614810;Name=Msa0614810
chr4_2 mRNA 63943214 63945022 63943214 ID=Msa0614810-mRNA-1;Parent=Msa0614810;Name=Msa0614810-mRNA-1;_AED=0.24;_eAED=0.25;_QI=0|0|0|1|1|1|5|0|333
chr4_2 exon 63943214 63943268 63943214 ID=Msa0614810-mRNA-1:exon:17910;Parent=Msa0614810-mRNA-1
chr4_2 exon 63943452 63943789 63943452 ID=Msa0614810-mRNA-1:exon:17911;Parent=Msa0614810-mRNA-1
chr4_2 exon 63943859 63944023 63943859 ID=Msa0614810-mRNA-1:exon:17912;Parent=Msa0614810-mRNA-1
chr4_2 exon 63944499 63944634 63944499 ID=Msa0614810-mRNA-1:exon:17913;Parent=Msa0614810-mRNA-1
chr4_2 exon 63944715 63945022 63944715 ID=Msa0614810-mRNA-1:exon:17914;Parent=Msa0614810-mRNA-1
chr4_2 CDS 63943214 63943268 63943214 ID=Msa0614810-mRNA-1:cds;Parent=Msa0614810-mRNA-1
chr4_2 CDS 63943452 63943789 63943452 ID=Msa0614810-mRNA-1:cds;Parent=Msa0614810-mRNA-1
chr4_2 CDS 63943859 63944023 63943859 ID=Msa0614810-mRNA-1:cds;Parent=Msa0614810-mRNA-1
chr4_2 CDS 63944499 63944634 63944499 ID=Msa0614810-mRNA-1:cds;Parent=Msa0614810-mRNA-1
chr4_2 CDS 63944715 63945022 63944715 ID=Msa0614810-mRNA-1:cds;Parent=Msa0614810-mRNA-1
Gene Sequence

>Msa0614810

ATGGCTATGTCGTGCGGGGGGCTAATCATTTTCGCCGCCTTTGTAACACTGGTATACACCGTTGTAGCTTCTAATTCCTCCTCCTCCTCCTCATCATCATCATCATTCCATTACATAATCACCGGAGTTAGTCCTGAGAATAACTGTCCTATCATCAATTTCCATTCTCTTCCATACGAAAACGTCTCCTTTACCGAACCTAATCATGAAGAATATATGGTGGTTGTGAGATGCAAAAAACTGGTAGATCATTACGGGTATATAGACATTAATTCTGAGAGTTGTGGTGGAAGAATTGACGTGAAGGGAAAGCAATGTTATTCATATATTGTCGCGACTGCTGACGGAAAATTTGTGTCAGACATAGAAGAGTCATGCAGAATAGAGATGATGGTTCACAGTGAGTACGTGAATGGAATTGAGTTTCGTTGGCGACCATTACGCTGTGAGCAAGACAGAGACAAAGACGGAGACTGGTATGGCGAAGGGAACAATAATTACCACTCCGCTGCACTCTCCCCGATGCAGCAGTCACTTTTCTTGCTATCGGATCACTTCTCCCGAACCGATGGACAAGACTTCATCAACGAAGTTGCAACCATTGGAAGAATTCGTCATGTCAATGTGGTGCAATTAATTGGATTTTGTGCTGAAAGAACAAAGCCAGCTCTCGTCTACGAGTTCATGCCCAATGATTACTTTGCTAAATGGATTTATGATCAATTTGAGGAAACAATAGATGCAAACAATGTAACAGAGGAGGAAAAGAATATTGCAATGAAGATGATCGTCATTGCAGCGAAATGCATACAAATGAAGCCCAATGACCGGCCCTCGATGAATGAAGTGATAGAAATGCTTGAAGGAGATGAAGTGCCCCAATACCTTCCACCTGATCCACAATCTCAGTTAACATGGCAAAGGATGTCAACAGATGGAAATGAGTTTAATAACAACCAACACTCATCAATGATTAGCGGAGTGGAAGTGCAATCAAAATGA
Protein sequence

>Msa0614810

MAMSCGGLIIFAAFVTLVYTVVASNSSSSSSSSSSFHYIITGVSPENNCPIINFHSLPYENVSFTEPNHEEYMVVVRCKKLVDHYGYIDINSESCGGRIDVKGKQCYSYIVATADGKFVSDIEESCRIEMMVHSEYVNGIEFRWRPLRCEQDRDKDGDWYGEGNNNYHSAALSPMQQSLFLLSDHFSRTDGQDFINEVATIGRIRHVNVVQLIGFCAERTKPALVYEFMPNDYFAKWIYDQFEETIDANNVTEEEKNIAMKMIVIAAKCIQMKPNDRPSMNEVIEMLEGDEVPQYLPPDPQSQLTWQRMSTDGNEFNNNQHSSMISGVEVQSK*