AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0616140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616140 MtrunA17_Chr8g0375251 96.907 679 21 0 1 679 93 771 0.0 1368
Msa0616140 MtrunA17_Chr4g0063461 57.851 121 34 1 25 145 7 110 8.52e-43 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616140 AT2G02160.1 39.936 626 285 27 3 596 5 571 2.43e-104 332

Find 217 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAGTCCTTCTTTCTATT+TGG 0.122370 4_2:-65840758 None:intergenic
ATTTGGGCTGATCCCTTTCT+AGG 0.173898 4_2:-65840741 None:intergenic
GAGAGAGCCGCCACAGAAAT+TGG 0.190492 4_2:-65838463 None:intergenic
GATCCATCGATCTCTTCTTC+TGG 0.192710 4_2:+65838526 Msa0616140:five_prime_UTR
GAATGTGATGATGGGCTTTA+AGG 0.209581 4_2:-65838376 None:intergenic
AGAATTTCACTAAGAGGTTT+TGG 0.245906 4_2:-65841332 None:intergenic
TATTAACTCTCTAACGTTAC+AGG 0.259234 4_2:-65841954 None:intergenic
TATCATGGGGAAGATGAATT+TGG 0.267482 4_2:+65840555 Msa0616140:CDS
GCTTGTTTACTACCATTATA+AGG 0.280011 4_2:-65840021 None:intergenic
TTTCTCAGAGAGTCGTCTCC+TGG 0.281678 4_2:+65840489 Msa0616140:CDS
CCTCTTCTCAGATTGAGATT+TGG 0.282214 4_2:+65838504 Msa0616140:five_prime_UTR
AGTCTTGCTGAACTTAAGTA+TGG 0.284200 4_2:+65841215 Msa0616140:CDS
ATGAGTCCTTCTTTCTATTT+GGG 0.297001 4_2:-65840757 None:intergenic
TTCTCCCTTGACATGCAAAA+AGG 0.299512 4_2:+65838668 Msa0616140:CDS
TCCTCTTTCCAAATGATTGT+TGG 0.310747 4_2:-65841259 None:intergenic
ATCCATCGATCTCTTCTTCT+GGG 0.318828 4_2:+65838527 Msa0616140:five_prime_UTR
TTGGAAAGAGGAAAAGCTTC+AGG 0.319306 4_2:+65841270 Msa0616140:CDS
ATCGATCTCTTCTTCTGGGT+TGG 0.324812 4_2:+65838531 Msa0616140:five_prime_UTR
CAGCCTCTTGACGGGTTGTT+AGG 0.337201 4_2:+65839916 Msa0616140:intron
CAACACCTGCAACATTTGCT+TGG 0.342566 4_2:-65840242 None:intergenic
TTAAGCATGCAGGCCTCAAC+TGG 0.343280 4_2:+65841814 Msa0616140:three_prime_UTR
CCAAAAGGTTTCTTAAAACT+TGG 0.344005 4_2:-65840183 None:intergenic
TCGATCTCTTCTTCTGGGTT+GGG 0.344716 4_2:+65838532 Msa0616140:five_prime_UTR
AAGAACAACACCAATCTTCT+TGG 0.345542 4_2:-65841693 None:intergenic
ACCCATGCACCGCCTTATAA+TGG 0.352929 4_2:+65840009 Msa0616140:CDS
GGAAGAAGAGTCCAAGATTC+AGG 0.353435 4_2:+65841463 Msa0616140:CDS
CCGGCAACTGCTGCTGCTGC+TGG 0.354940 4_2:+65839946 Msa0616140:CDS
GCCGTTTCCCTTCCTGCGCT+TGG 0.358186 4_2:-65840835 None:intergenic
ATCAGTGGCCGGTTGTCATC+TGG 0.358804 4_2:+65841068 Msa0616140:CDS
TCGTATTCATTCAAAGAGTC+TGG 0.359110 4_2:-65840606 None:intergenic
AGAAATTCTGATTACTATCA+TGG 0.361394 4_2:+65840540 Msa0616140:CDS
AGAGTAAGAGAATGTGATGA+TGG 0.369394 4_2:-65838385 None:intergenic
TTACTGCCATTGGTAGGGTT+TGG 0.386685 4_2:-65840111 None:intergenic
AATAATGACCAACAATCATT+TGG 0.389220 4_2:+65841251 Msa0616140:CDS
GCCTCATTATCTAACCCTTC+AGG 0.391125 4_2:-65840342 None:intergenic
CGTCATTGTCCTTACCACTA+TGG 0.395917 4_2:-65840464 None:intergenic
ATCTCTCATTTGAAGGCAAC+TGG 0.399075 4_2:-65840920 None:intergenic
TCCTTCAGAATTTCACTAAG+AGG 0.399426 4_2:-65841338 None:intergenic
ATCCTAGAGATTGTTGGTAT+TGG 0.401206 4_2:+65839718 Msa0616140:CDS
ATCATATGAGTCATAACCTT+GGG 0.402504 4_2:-65840691 None:intergenic
TCCATCAGCAGCCTGAATCT+TGG 0.402576 4_2:-65841474 None:intergenic
CTATGGAAATTGTGATCATC+TGG 0.404436 4_2:-65840447 None:intergenic
AAGTTTCTCCTCTCGTCATC+TGG 0.405913 4_2:-65841146 None:intergenic
CGATGAAGCTGCCGAAGATC+AGG 0.408429 4_2:+65841550 Msa0616140:CDS
AAATCAAGAGCAAGAATATA+TGG 0.412298 4_2:+65841646 Msa0616140:CDS
GTACATCATCTTCATATTGT+TGG 0.423888 4_2:-65841298 None:intergenic
TTTCTAGGTTCCCAACCATA+TGG 0.426114 4_2:-65840726 None:intergenic
TTGACGGATCTCTCATTTGA+AGG 0.431022 4_2:-65840927 None:intergenic
TAAAACAAGTAAGCGTGGTT+TGG 0.434567 4_2:-65838353 None:intergenic
AACTTGGATTCTCAGTTCCT+TGG 0.435011 4_2:-65840167 None:intergenic
AGGATCCAGGATAGATTAAG+AGG 0.436261 4_2:+65841116 Msa0616140:CDS
ACTTCCCAAGCAAATGTTGC+AGG 0.436467 4_2:+65840237 Msa0616140:CDS
ATTGGTTGGCGGTGGAGAAC+TGG 0.437841 4_2:-65840382 None:intergenic
ACCTTCATACTCCTGATCTT+CGG 0.437886 4_2:-65841561 None:intergenic
GAAGATGATGTACCATTTGA+AGG 0.438650 4_2:+65841308 Msa0616140:CDS
GAATCTGAAGTTGTGGCTGT+TGG 0.444668 4_2:-65838572 None:intergenic
ACCTTCCTCTTAATCTATCC+TGG 0.445108 4_2:-65841121 None:intergenic
AACACCTGCAACATTTGCTT+GGG 0.445165 4_2:-65840241 None:intergenic
AGGTGACTGTCACCACCATT+TGG 0.450256 4_2:-65840981 None:intergenic
TGGATTCTCAGTTCCTTGGT+TGG 0.451049 4_2:-65840163 None:intergenic
GAAATTCTGATTACTATCAT+GGG 0.451701 4_2:+65840541 Msa0616140:CDS
GTCCCTCTCTAGGTGACTGC+TGG 0.452700 4_2:-65841030 None:intergenic
ACACGATTAGACCGGGCATT+AGG 0.453044 4_2:-65840408 None:intergenic
GATTGTTGGTATTGGTTGAA+TGG 0.456384 4_2:+65839726 Msa0616140:CDS
ATCTGTGGCGTGGGGACAGA+TGG 0.457178 4_2:-65839970 None:intergenic
TAAGTTAAAGAGACAATATA+AGG 0.459796 4_2:-65841776 None:intergenic
CGGATGACGGAATGAACACT+TGG 0.466296 4_2:-65839763 None:intergenic
CTCAAGATGAAGCCTTGAAA+AGG 0.468804 4_2:+65838619 Msa0616140:CDS
AATATATGGAAGATGAAGAT+GGG 0.468862 4_2:+65841660 Msa0616140:CDS
AGAGAGCCGCCACAGAAATT+GGG 0.468937 4_2:-65838462 None:intergenic
AATCTGAAGTTGTGGCTGTT+GGG 0.469633 4_2:-65838571 None:intergenic
TGACTCTAGCATACTCACTA+TGG 0.470886 4_2:-65839695 None:intergenic
AGTTCTCCACCGCCAACCAA+TGG 0.471253 4_2:+65840384 Msa0616140:CDS
ATATGGTTGGGAACCTAGAA+AGG 0.473339 4_2:+65840728 Msa0616140:CDS
TCTGAGACCTCAACACTGTC+AGG 0.480290 4_2:-65840795 None:intergenic
GCATTAGGCCCATTGGTTGG+CGG 0.480720 4_2:-65840393 None:intergenic
AGAATAAATCTTCCACCAAA+TGG 0.481461 4_2:+65840966 Msa0616140:CDS
AATGAAGGAATTACCGAAAA+CGG 0.483376 4_2:+65841422 Msa0616140:CDS
GATGACTTTGCCAAGAAGAT+TGG 0.484215 4_2:+65841683 Msa0616140:CDS
GCCTCATGAGGGAAGGATCC+AGG 0.484648 4_2:+65841103 Msa0616140:CDS
TACCATTATAAGGCGGTGCA+TGG 0.485504 4_2:-65840011 None:intergenic
CAACCAATGGGCCTAATGCC+CGG 0.485721 4_2:+65840397 Msa0616140:CDS
GTTCTCCACCGCCAACCAAT+GGG 0.486313 4_2:+65840385 Msa0616140:CDS
TTAGGCCCATTGGTTGGCGG+TGG 0.486496 4_2:-65840390 None:intergenic
TTCTCAGAGAGTCGTCTCCT+GGG 0.488365 4_2:+65840490 Msa0616140:CDS
GAAATTCTGAAGGAGAAGAA+AGG 0.491627 4_2:+65841347 Msa0616140:CDS
TCCAAGCGCAGGAAGGGAAA+CGG 0.492405 4_2:+65840834 Msa0616140:CDS
GAATATATGGAAGATGAAGA+TGG 0.492589 4_2:+65841659 Msa0616140:CDS
GCCTCGGAAACGATTATTGA+CGG 0.493252 4_2:-65840943 None:intergenic
CAAGTGTTCATTCCGTCATC+CGG 0.493411 4_2:+65839764 Msa0616140:CDS
TTTACATGCAGCCTCTTGAC+GGG 0.494749 4_2:+65839908 Msa0616140:intron
GTCATCATGTTTGCTACTGT+GGG 0.496484 4_2:-65841393 None:intergenic
TGGTAGGGTTTGGTCCATGC+AGG 0.497954 4_2:-65840101 None:intergenic
TCCAAGATTCAGGCTGCTGA+TGG 0.498699 4_2:+65841473 Msa0616140:CDS
GAAGCCTTCCAGATGACAAC+CGG 0.499100 4_2:-65841076 None:intergenic
CTGGGTTGGGTTGTTCGCCA+TGG 0.501508 4_2:+65838545 Msa0616140:exon
TTATCTAACCCTTCAGGTGG+TGG 0.501541 4_2:-65840336 None:intergenic
AAGGCTTCATCTTGAGGAGA+AGG 0.502963 4_2:-65838612 None:intergenic
TCATCTTGAGGAGAAGGTGT+TGG 0.504643 4_2:-65838606 None:intergenic
TGGAAAGAGGAAAAGCTTCA+GGG 0.504669 4_2:+65841271 Msa0616140:CDS
CTGAAGGAGAAGAAAGGAGT+TGG 0.505461 4_2:+65841353 Msa0616140:CDS
GTATGAGTATGAACAAGTTG+AGG 0.507239 4_2:+65841619 Msa0616140:CDS
GAGAAGAGGAGAAGAAGTAG+AGG 0.508002 4_2:-65838490 None:intergenic
TCTTTGAATGAATACGATGT+AGG 0.510886 4_2:+65840612 Msa0616140:CDS
AAAGGAGTTGGAGCTGGTGC+AGG 0.510993 4_2:+65841365 Msa0616140:CDS
CATGATGACAATAAGAATGA+AGG 0.513449 4_2:+65841407 Msa0616140:CDS
GAGTAAGAGAATGTGATGAT+GGG 0.516605 4_2:-65838384 None:intergenic
CATGCAAGAGCCATATGGTT+GGG 0.517627 4_2:+65840716 Msa0616140:CDS
AGACCGGGCATTAGGCCCAT+TGG 0.518925 4_2:-65840400 None:intergenic
ACCAACAATCATTTGGAAAG+AGG 0.519008 4_2:+65841258 Msa0616140:CDS
TGTCATCATGTTTGCTACTG+TGG 0.520700 4_2:-65841394 None:intergenic
CTTTGAATGAATACGATGTA+GGG 0.521877 4_2:+65840613 Msa0616140:CDS
CGGAACATTGTCAGAGAGCA+AGG 0.523049 4_2:+65841442 Msa0616140:CDS
GAGTCAATCCTAGAGATTGT+TGG 0.524919 4_2:+65839712 Msa0616140:CDS
TATGAGTATGAACAAGTTGA+GGG 0.525313 4_2:+65841620 Msa0616140:CDS
GTTGCCGCAATCTGTGGCGT+GGG 0.527866 4_2:-65839979 None:intergenic
GATCACATGCAAGAGCCATA+TGG 0.529220 4_2:+65840711 Msa0616140:CDS
ACCTGAAGGGTTAGATAATG+AGG 0.533549 4_2:+65840341 Msa0616140:CDS
GCAGCAGCAGCAGTTGCCGG+TGG 0.534503 4_2:-65839943 None:intergenic
GTGGAAGATTTATTCTGCCT+CGG 0.535587 4_2:-65840959 None:intergenic
GCTTCCATCATATCAACTAA+AGG 0.541333 4_2:-65842004 None:intergenic
GAGAAGAAAGGAGTTGGAGC+TGG 0.544404 4_2:+65841359 Msa0616140:CDS
CTCTGTCCAAGTCCCTCTCT+AGG 0.544981 4_2:-65841040 None:intergenic
CGTTTATCCAAGCGCAGGAA+GGG 0.547030 4_2:+65840828 Msa0616140:CDS
ACCATTATAAGGCGGTGCAT+GGG 0.548023 4_2:-65840010 None:intergenic
GATGATCACAATTTCCATAG+TGG 0.554285 4_2:+65840450 Msa0616140:CDS
TGGCAGTAAAATAGCTACAC+AGG 0.556312 4_2:+65840125 Msa0616140:CDS
ACCTCTTAGTGAAATTCTGA+AGG 0.557787 4_2:+65841337 Msa0616140:CDS
AGCAAGAAAGTAGACACAAT+CGG 0.559051 4_2:-65838646 None:intergenic
GATCGAATGAGGACCGAAGT+GGG 0.559665 4_2:+65841177 Msa0616140:CDS
TCCAGGATAGATTAAGAGGA+AGG 0.562641 4_2:+65841120 Msa0616140:CDS
CGGGCATTAGGCCCATTGGT+TGG 0.564557 4_2:-65840396 None:intergenic
CTCTGTCTAAGTCCCTCTCT+AGG 0.564616 4_2:-65841001 None:intergenic
TCCTGGATCCTTCCCTCATG+AGG 0.568928 4_2:-65841104 None:intergenic
GAACCAGCAGTCACCTAGAG+AGG 0.569215 4_2:+65841027 Msa0616140:CDS
TGTCCCCACGCCACAGATTG+CGG 0.569632 4_2:+65839975 Msa0616140:CDS
TGACTGTCACCACCATTTGG+TGG 0.569677 4_2:-65840978 None:intergenic
CGTATTCATTCAAAGAGTCT+GGG 0.569875 4_2:-65840605 None:intergenic
GACAGATGTGCCTTCCTGCA+TGG 0.570126 4_2:+65840087 Msa0616140:CDS
CCAAATCTCAATCTGAGAAG+AGG 0.573248 4_2:-65838504 None:intergenic
TTGAAAGAAAATGCAAGGCA+CGG 0.577753 4_2:-65840043 None:intergenic
CTTCAGCAGTCGATCGAATG+AGG 0.577765 4_2:+65841166 Msa0616140:CDS
TGTTGCCGCAATCTGTGGCG+TGG 0.581148 4_2:-65839980 None:intergenic
GACAGATATTACCCCAGTTG+AGG 0.582522 4_2:-65841827 None:intergenic
GCAGATGTTGCCGCAATCTG+TGG 0.582954 4_2:-65839985 None:intergenic
TCGTTTATCCAAGCGCAGGA+AGG 0.583309 4_2:+65840827 Msa0616140:CDS
GATCAGCCCAAATAGAAAGA+AGG 0.583430 4_2:+65840751 Msa0616140:CDS
GATGAATTTGGTAAAACAAG+AGG 0.583764 4_2:+65840567 Msa0616140:CDS
CATGTACCGCCACCACCTGA+AGG 0.584923 4_2:+65840327 Msa0616140:CDS
TCACAATTTCCATAGTGGTA+AGG 0.586818 4_2:+65840455 Msa0616140:CDS
TTGTGGCTGTTGGGCAACCA+TGG 0.587839 4_2:-65838562 None:intergenic
TCGTAGTCCTGACAGTGTTG+AGG 0.587874 4_2:+65840788 Msa0616140:CDS
GTTGATGCACACGATTAGAC+CGG 0.589529 4_2:-65840416 None:intergenic
TAAGCATGCAGGCCTCAACT+GGG 0.590264 4_2:+65841815 Msa0616140:three_prime_UTR
TTCAATCGCCTCATGAGGGA+AGG 0.590666 4_2:+65841096 Msa0616140:CDS
GATCATATGAGTCATAACCT+TGG 0.590754 4_2:-65840692 None:intergenic
CAGTCACCTAGAGAGGGACT+TGG 0.591478 4_2:+65841034 Msa0616140:CDS
TCTAACCCTTCAGGTGGTGG+CGG 0.591953 4_2:-65840333 None:intergenic
ACATGCAAGAGCCATATGGT+TGG 0.592563 4_2:+65840715 Msa0616140:CDS
AAGGCTTCAATCGCCTCATG+AGG 0.592807 4_2:+65841091 Msa0616140:CDS
ATCTGTCGTCGCTCATGATG+AGG 0.592954 4_2:+65840863 Msa0616140:CDS
AACCAATACCAACAATCTCT+AGG 0.593370 4_2:-65839720 None:intergenic
CGATCGAATGAGGACCGAAG+TGG 0.593485 4_2:+65841176 Msa0616140:CDS
GACATCGTTTATCCAAGCGC+AGG 0.594290 4_2:+65840823 Msa0616140:CDS
GTCTTGCTGAACTTAAGTAT+GGG 0.596178 4_2:+65841216 Msa0616140:CDS
GATCAGGAGTATGAAGGTGA+AGG 0.597456 4_2:+65841566 Msa0616140:CDS
GCCGAAGATCAGGAGTATGA+AGG 0.599647 4_2:+65841560 Msa0616140:CDS
TCTCTACCCAATTTCTGTGG+CGG 0.599837 4_2:+65838456 Msa0616140:five_prime_UTR
CACGGTCAATCATCTGAGGA+AGG 0.602447 4_2:+65841518 Msa0616140:CDS
TAAAGAAACTCACCGGATGA+CGG 0.603837 4_2:-65839776 None:intergenic
CTAGAGAGGGACTTGGACAG+AGG 0.604960 4_2:+65841041 Msa0616140:CDS
CGGGTTGTTAGGAACACCAC+CGG 0.605352 4_2:+65839927 Msa0616140:CDS
TGAATATGAGCAAGGTGATG+AGG 0.606447 4_2:+65841595 Msa0616140:CDS
GTTCCTAACAACCCGTCAAG+AGG 0.607813 4_2:-65839919 None:intergenic
ATCATGTTTGCTACTGTGGG+AGG 0.610033 4_2:-65841390 None:intergenic
GATAGATTTCGTGTGCCCCA+AGG 0.610131 4_2:+65840675 Msa0616140:CDS
TATGGTTGGGAACCTAGAAA+GGG 0.612471 4_2:+65840729 Msa0616140:CDS
ACAAGAGGTCAAGATGAAAG+AGG 0.612476 4_2:+65840582 Msa0616140:CDS
ATATATGGAAGATGAAGATG+GGG 0.612975 4_2:+65841661 Msa0616140:CDS
CTCTCTCTACCCAATTTCTG+TGG 0.614772 4_2:+65838453 Msa0616140:five_prime_UTR
GACAATACTGATGTAACTCA+CGG 0.620169 4_2:+65841500 Msa0616140:CDS
TTGCTGAACTTAAGTATGGG+AGG 0.621411 4_2:+65841219 Msa0616140:CDS
GTGGTGACAGTCACCTAGAG+AGG 0.623462 4_2:+65840988 Msa0616140:CDS
ACAGAGGAAGAATCAGTGGC+CGG 0.626828 4_2:+65841057 Msa0616140:CDS
TTGTGATCATCTGGCAAACG+AGG 0.626895 4_2:-65840438 None:intergenic
TCCGTCAATAATCGTTTCCG+AGG 0.626973 4_2:+65840942 Msa0616140:CDS
TTGGCGCAGAGAACCCACTT+CGG 0.629325 4_2:-65841190 None:intergenic
GGCAGTAAAATAGCTACACA+GGG 0.630874 4_2:+65840126 Msa0616140:CDS
AGGGTTTGGTCCATGCAGGA+AGG 0.631683 4_2:-65840097 None:intergenic
AACCCAGAAGAAGAGATCGA+TGG 0.631981 4_2:-65838529 None:intergenic
ATGTACCGCCACCACCTGAA+GGG 0.632419 4_2:+65840328 Msa0616140:CDS
AACCAGCAGTCACCTAGAGA+GGG 0.636906 4_2:+65841028 Msa0616140:CDS
TAAGGTAAAACAAGTAAGCG+TGG 0.639199 4_2:-65838358 None:intergenic
GAAGGTTGCCAGATGACGAG+AGG 0.639411 4_2:+65841138 Msa0616140:CDS
CTAGAGAGGGACTTAGACAG+AGG 0.639929 4_2:+65841002 Msa0616140:CDS
GGATGACGGAATGAACACTT+GGG 0.640470 4_2:-65839762 None:intergenic
AGGCTTCAATCGCCTCATGA+GGG 0.642774 4_2:+65841092 Msa0616140:CDS
AAATTCTGATTACTATCATG+GGG 0.645440 4_2:+65840542 Msa0616140:CDS
TTGCCGCAATCTGTGGCGTG+GGG 0.646254 4_2:-65839978 None:intergenic
GTGGCCGGTTGTCATCTGGA+AGG 0.646666 4_2:+65841072 Msa0616140:CDS
CATGGACCAAACCCTACCAA+TGG 0.648418 4_2:+65840105 Msa0616140:CDS
ATAAATCTTCCACCAAATGG+TGG 0.648459 4_2:+65840969 Msa0616140:CDS
TTGGACAGAGGAAGAATCAG+TGG 0.649407 4_2:+65841053 Msa0616140:CDS
GGAGACTATGAATATGAGCA+AGG 0.650461 4_2:+65841587 Msa0616140:CDS
TTGATGCACACGATTAGACC+GGG 0.654044 4_2:-65840415 None:intergenic
TGCGCTTGGATAAACGATGT+CGG 0.656360 4_2:-65840821 None:intergenic
AACTCACGGTCAATCATCTG+AGG 0.657007 4_2:+65841514 Msa0616140:CDS
TCAATGACAATGACCAACCA+AGG 0.662551 4_2:+65840150 Msa0616140:CDS
TGAGTCTGAATCTGAAGTTG+TGG 0.677604 4_2:-65838579 None:intergenic
TGGTGACAGTCACCTAGAGA+GGG 0.684816 4_2:+65840989 Msa0616140:CDS
TGTTTACTACCATTATAAGG+CGG 0.686484 4_2:-65840018 None:intergenic
TCATTATCTAACCCTTCAGG+TGG 0.688573 4_2:-65840339 None:intergenic
AAGCATGCAGGCCTCAACTG+GGG 0.704027 4_2:+65841816 Msa0616140:three_prime_UTR
TCATATGAGTCATAACCTTG+GGG 0.704228 4_2:-65840690 None:intergenic
CTCTAGCATACTCACTATGG+CGG 0.707134 4_2:-65839692 None:intergenic
GCTACAAGAACATCAAACCC+AGG 0.714968 4_2:-65840507 None:intergenic
GCAGTAAAATAGCTACACAG+GGG 0.802739 4_2:+65840127 Msa0616140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTAATAATAATA+GGG - chr4_2:65839321-65839340 None:intergenic 0.0%
!! ATAATTGATAAAGAAAAAAA+GGG - chr4_2:65838725-65838744 None:intergenic 10.0%
!! TATAATTGATAAAGAAAAAA+AGG - chr4_2:65838726-65838745 None:intergenic 10.0%
!!! TTTATCAATTATATCTTTTA+CGG + chr4_2:65838733-65838752 Msa0616140:intron 10.0%
!!! AAATTTAATTTTGATTAATG+GGG + chr4_2:65838815-65838834 Msa0616140:intron 10.0%
!! CATAACTCAATTAAAATAAA+AGG - chr4_2:65839820-65839839 None:intergenic 15.0%
!!! TTGTTCTTTCATAATTTTTA+TGG + chr4_2:65841708-65841727 Msa0616140:exon 15.0%
!!! TCTACAATTCAACAATTTTA+GGG - chr4_2:65838877-65838896 None:intergenic 20.0%
!!! ATCTACAATTCAACAATTTT+AGG - chr4_2:65838878-65838897 None:intergenic 20.0%
!! AATACTCTAAACTTAATGAA+AGG - chr4_2:65839230-65839249 None:intergenic 20.0%
!!! AGTTTAGAGTATTATACATA+AGG + chr4_2:65839237-65839256 Msa0616140:intron 20.0%
!!! GTTTAGAGTATTATACATAA+GGG + chr4_2:65839238-65839257 Msa0616140:intron 20.0%
!! CATCTGTTATTAAGAATATA+AGG + chr4_2:65839570-65839589 Msa0616140:intron 20.0%
!!! AACTTAAACATATTTTTCTG+TGG - chr4_2:65840300-65840319 None:intergenic 20.0%
!! ACAGAAAAATATGTTTAAGT+TGG + chr4_2:65840299-65840318 Msa0616140:CDS 20.0%
!! TAAGTTAAAGAGACAATATA+AGG - chr4_2:65841779-65841798 None:intergenic 20.0%
!!! TGATATTTTTATGTAAGAAG+TGG + chr4_2:65841898-65841917 Msa0616140:three_prime_UTR 20.0%
!!! GATATTTTTATGTAAGAAGT+GGG + chr4_2:65841899-65841918 Msa0616140:three_prime_UTR 20.0%
!!! ATATTTTTATGTAAGAAGTG+GGG + chr4_2:65841900-65841919 Msa0616140:three_prime_UTR 20.0%
!!! AAAAAGGGCTTTTTAGAAAA+AGG - chr4_2:65838710-65838729 None:intergenic 25.0%
!!! TTTCAGATTCGAATTAGTTT+TGG + chr4_2:65838756-65838775 Msa0616140:intron 25.0%
!!! TAATTTTGATTAATGGGGTT+TGG + chr4_2:65838820-65838839 Msa0616140:intron 25.0%
! ACAAAAAAATCTTCATGGAT+GGG - chr4_2:65838903-65838922 None:intergenic 25.0%
! AACAAAAAAATCTTCATGGA+TGG - chr4_2:65838904-65838923 None:intergenic 25.0%
! GTCTAACAAAAAAATCTTCA+TGG - chr4_2:65838908-65838927 None:intergenic 25.0%
!!! GTTAGACTGTGAATTTTTTA+TGG + chr4_2:65838921-65838940 Msa0616140:intron 25.0%
!!! TGATCATCTTGTTATTTGAT+TGG + chr4_2:65838971-65838990 Msa0616140:intron 25.0%
! AATAAGCTATTTATCCAAAC+AGG + chr4_2:65839294-65839313 Msa0616140:intron 25.0%
! ATAAGCTATTTATCCAAACA+GGG + chr4_2:65839295-65839314 Msa0616140:intron 25.0%
! AGTATTTGAGTCTTGATTAT+AGG + chr4_2:65839353-65839372 Msa0616140:intron 25.0%
! TTCTCTTCTTTATTGAAACA+GGG + chr4_2:65839654-65839673 Msa0616140:intron 25.0%
! AGAAATTCTGATTACTATCA+TGG + chr4_2:65840540-65840559 Msa0616140:CDS 25.0%
! GAAATTCTGATTACTATCAT+GGG + chr4_2:65840541-65840560 Msa0616140:CDS 25.0%
! AAATTCTGATTACTATCATG+GGG + chr4_2:65840542-65840561 Msa0616140:CDS 25.0%
! AATAATGACCAACAATCATT+TGG + chr4_2:65841251-65841270 Msa0616140:CDS 25.0%
! AAATCAAGAGCAAGAATATA+TGG + chr4_2:65841646-65841665 Msa0616140:CDS 25.0%
! AATATATGGAAGATGAAGAT+GGG + chr4_2:65841660-65841679 Msa0616140:CDS 25.0%
!!! TTTTCCTTTAGTTGATATGA+TGG + chr4_2:65842000-65842019 Msa0616140:three_prime_UTR 25.0%
!! TTTTTATGGATTGCGTTTCT+TGG + chr4_2:65838935-65838954 Msa0616140:intron 30.0%
AAAGGAATGATCAAAAAGAG+AGG - chr4_2:65839017-65839036 None:intergenic 30.0%
! TCCTTTTGTATTTAACAGAG+TGG + chr4_2:65839031-65839050 Msa0616140:intron 30.0%
! CCTTTTGTATTTAACAGAGT+GGG + chr4_2:65839032-65839051 Msa0616140:intron 30.0%
AGTTAACAAAAACAACTTCC+AGG - chr4_2:65839412-65839431 None:intergenic 30.0%
!!! ACATGCTGCTTTTATTTTTG+TGG + chr4_2:65839499-65839518 Msa0616140:intron 30.0%
GTTCTCTTCTTTATTGAAAC+AGG + chr4_2:65839653-65839672 Msa0616140:intron 30.0%
AGTCAAAAGATAGGACAAAA+TGG - chr4_2:65839855-65839874 None:intergenic 30.0%
ATACAGACAAGTCAAAAGAT+AGG - chr4_2:65839864-65839883 None:intergenic 30.0%
!!! TATCTTTTGACTTGTCTGTA+TGG + chr4_2:65839863-65839882 Msa0616140:intron 30.0%
TGTTTACTACCATTATAAGG+CGG - chr4_2:65840021-65840040 None:intergenic 30.0%
GCTTGTTTACTACCATTATA+AGG - chr4_2:65840024-65840043 None:intergenic 30.0%
! CCTTGCATTTTCTTTCAAAA+GGG + chr4_2:65840048-65840067 Msa0616140:CDS 30.0%
! CTTGCATTTTCTTTCAAAAG+GGG + chr4_2:65840049-65840068 Msa0616140:CDS 30.0%
CCAAAAGGTTTCTTAAAACT+TGG - chr4_2:65840186-65840205 None:intergenic 30.0%
!!! CCAAGTTTTAAGAAACCTTT+TGG + chr4_2:65840183-65840202 Msa0616140:CDS 30.0%
!!! AGTTTTAAGAAACCTTTTGG+CGG + chr4_2:65840186-65840205 Msa0616140:CDS 30.0%
TTGAAAAACATGCACAAGAA+AGG + chr4_2:65840211-65840230 Msa0616140:CDS 30.0%
GATGAATTTGGTAAAACAAG+AGG + chr4_2:65840567-65840586 Msa0616140:CDS 30.0%
TCTTTGAATGAATACGATGT+AGG + chr4_2:65840612-65840631 Msa0616140:CDS 30.0%
CTTTGAATGAATACGATGTA+GGG + chr4_2:65840613-65840632 Msa0616140:CDS 30.0%
ATCATATGAGTCATAACCTT+GGG - chr4_2:65840694-65840713 None:intergenic 30.0%
ATGAGTCCTTCTTTCTATTT+GGG - chr4_2:65840760-65840779 None:intergenic 30.0%
AGAATAAATCTTCCACCAAA+TGG + chr4_2:65840966-65840985 Msa0616140:CDS 30.0%
! TTAAGTTCAGCAAGACTTTT+TGG - chr4_2:65841212-65841231 None:intergenic 30.0%
GTACATCATCTTCATATTGT+TGG - chr4_2:65841301-65841320 None:intergenic 30.0%
! AGAATTTCACTAAGAGGTTT+TGG - chr4_2:65841335-65841354 None:intergenic 30.0%
CATGATGACAATAAGAATGA+AGG + chr4_2:65841407-65841426 Msa0616140:CDS 30.0%
AATGAAGGAATTACCGAAAA+CGG + chr4_2:65841422-65841441 Msa0616140:CDS 30.0%
! TATGAGTATGAACAAGTTGA+GGG + chr4_2:65841620-65841639 Msa0616140:CDS 30.0%
GAATATATGGAAGATGAAGA+TGG + chr4_2:65841659-65841678 Msa0616140:CDS 30.0%
ATATATGGAAGATGAAGATG+GGG + chr4_2:65841661-65841680 Msa0616140:CDS 30.0%
TATTAACTCTCTAACGTTAC+AGG - chr4_2:65841957-65841976 None:intergenic 30.0%
TAAAACAAGTAAGCGTGGTT+TGG - chr4_2:65838356-65838375 None:intergenic 35.0%
TAAGGTAAAACAAGTAAGCG+TGG - chr4_2:65838361-65838380 None:intergenic 35.0%
GAGTAAGAGAATGTGATGAT+GGG - chr4_2:65838387-65838406 None:intergenic 35.0%
AGAGTAAGAGAATGTGATGA+TGG - chr4_2:65838388-65838407 None:intergenic 35.0%
AGCAAGAAAGTAGACACAAT+CGG - chr4_2:65838649-65838668 None:intergenic 35.0%
! AGAACCTTTTTGCATGTCAA+GGG - chr4_2:65838675-65838694 None:intergenic 35.0%
! AAGAACCTTTTTGCATGTCA+AGG - chr4_2:65838676-65838695 None:intergenic 35.0%
CCCACTCTGTTAAATACAAA+AGG - chr4_2:65839035-65839054 None:intergenic 35.0%
GTGAAGGAAATTAGGATGAA+CGG + chr4_2:65839054-65839073 Msa0616140:intron 35.0%
TGAAGGAAATTAGGATGAAC+GGG + chr4_2:65839055-65839074 Msa0616140:intron 35.0%
GAACCCTAATTGTGATTGTT+AGG + chr4_2:65839084-65839103 Msa0616140:intron 35.0%
GGTCCTAACAATCACAATTA+GGG - chr4_2:65839090-65839109 None:intergenic 35.0%
TGGTCCTAACAATCACAATT+AGG - chr4_2:65839091-65839110 None:intergenic 35.0%
CATGTGTATACTGTAGTGAA+TGG + chr4_2:65839204-65839223 Msa0616140:intron 35.0%
AATAATAATAGGGCCCTGTT+TGG - chr4_2:65839311-65839330 None:intergenic 35.0%
CAAGAAAACACAACTGCTAA+AGG - chr4_2:65839383-65839402 None:intergenic 35.0%
! TGTGTTTTCTTGAGTAATCC+TGG + chr4_2:65839391-65839410 Msa0616140:intron 35.0%
ATCCTAGAGATTGTTGGTAT+TGG + chr4_2:65839718-65839737 Msa0616140:CDS 35.0%
AACCAATACCAACAATCTCT+AGG - chr4_2:65839723-65839742 None:intergenic 35.0%
!! GATTGTTGGTATTGGTTGAA+TGG + chr4_2:65839726-65839745 Msa0616140:CDS 35.0%
TTGAAAGAAAATGCAAGGCA+CGG - chr4_2:65840046-65840065 None:intergenic 35.0%
! GCCTTGCATTTTCTTTCAAA+AGG + chr4_2:65840047-65840066 Msa0616140:CDS 35.0%
!! CCCTTTTGAAAGAAAATGCA+AGG - chr4_2:65840051-65840070 None:intergenic 35.0%
! CAAAAGGGGTTTTGCTTAAA+AGG + chr4_2:65840063-65840082 Msa0616140:CDS 35.0%
! CTATTTTACTGCCATTGGTA+GGG - chr4_2:65840119-65840138 None:intergenic 35.0%
!! TGTAGCTATTTTACTGCCAT+TGG - chr4_2:65840124-65840143 None:intergenic 35.0%
CTATGGAAATTGTGATCATC+TGG - chr4_2:65840450-65840469 None:intergenic 35.0%
GATGATCACAATTTCCATAG+TGG + chr4_2:65840450-65840469 Msa0616140:CDS 35.0%
TCACAATTTCCATAGTGGTA+AGG + chr4_2:65840455-65840474 Msa0616140:CDS 35.0%
TATCATGGGGAAGATGAATT+TGG + chr4_2:65840555-65840574 Msa0616140:CDS 35.0%
CGTATTCATTCAAAGAGTCT+GGG - chr4_2:65840608-65840627 None:intergenic 35.0%
TCGTATTCATTCAAAGAGTC+TGG - chr4_2:65840609-65840628 None:intergenic 35.0%
TCATATGAGTCATAACCTTG+GGG - chr4_2:65840693-65840712 None:intergenic 35.0%
GATCATATGAGTCATAACCT+TGG - chr4_2:65840695-65840714 None:intergenic 35.0%
GATGAGTCCTTCTTTCTATT+TGG - chr4_2:65840761-65840780 None:intergenic 35.0%
!!! AGCAAAGTCATCGATTTTCT+CGG + chr4_2:65840886-65840905 Msa0616140:CDS 35.0%
ATAAATCTTCCACCAAATGG+TGG + chr4_2:65840969-65840988 Msa0616140:CDS 35.0%
AGTCTTGCTGAACTTAAGTA+TGG + chr4_2:65841215-65841234 Msa0616140:CDS 35.0%
GTCTTGCTGAACTTAAGTAT+GGG + chr4_2:65841216-65841235 Msa0616140:CDS 35.0%
ACCAACAATCATTTGGAAAG+AGG + chr4_2:65841258-65841277 Msa0616140:CDS 35.0%
TCCTCTTTCCAAATGATTGT+TGG - chr4_2:65841262-65841281 None:intergenic 35.0%
GAAGATGATGTACCATTTGA+AGG + chr4_2:65841308-65841327 Msa0616140:CDS 35.0%
ACCTCTTAGTGAAATTCTGA+AGG + chr4_2:65841337-65841356 Msa0616140:CDS 35.0%
TCCTTCAGAATTTCACTAAG+AGG - chr4_2:65841341-65841360 None:intergenic 35.0%
! GAAATTCTGAAGGAGAAGAA+AGG + chr4_2:65841347-65841366 Msa0616140:CDS 35.0%
GACAATACTGATGTAACTCA+CGG + chr4_2:65841500-65841519 Msa0616140:CDS 35.0%
GTATGAGTATGAACAAGTTG+AGG + chr4_2:65841619-65841638 Msa0616140:CDS 35.0%
AAGAACAACACCAATCTTCT+TGG - chr4_2:65841696-65841715 None:intergenic 35.0%
!!! TTTTTTCTGCTTAAGCATGC+AGG + chr4_2:65841804-65841823 Msa0616140:three_prime_UTR 35.0%
GCTTCCATCATATCAACTAA+AGG - chr4_2:65842007-65842026 None:intergenic 35.0%
!! GAATGTGATGATGGGCTTTA+AGG - chr4_2:65838379-65838398 None:intergenic 40.0%
CCAAATCTCAATCTGAGAAG+AGG - chr4_2:65838507-65838526 None:intergenic 40.0%
CCTCTTCTCAGATTGAGATT+TGG + chr4_2:65838504-65838523 Msa0616140:five_prime_UTR 40.0%
ATCCATCGATCTCTTCTTCT+GGG + chr4_2:65838527-65838546 Msa0616140:five_prime_UTR 40.0%
! AATCTGAAGTTGTGGCTGTT+GGG - chr4_2:65838574-65838593 None:intergenic 40.0%
! TGAGTCTGAATCTGAAGTTG+TGG - chr4_2:65838582-65838601 None:intergenic 40.0%
!! CTTTTCAAGGCTTCATCTTG+AGG - chr4_2:65838621-65838640 None:intergenic 40.0%
CTCAAGATGAAGCCTTGAAA+AGG + chr4_2:65838619-65838638 Msa0616140:CDS 40.0%
!! ACAATCGGTGTTCCTTTTCA+AGG - chr4_2:65838634-65838653 None:intergenic 40.0%
TTCTCCCTTGACATGCAAAA+AGG + chr4_2:65838668-65838687 Msa0616140:CDS 40.0%
GTATTTAACAGAGTGGGTGA+AGG + chr4_2:65839038-65839057 Msa0616140:intron 40.0%
GAAGGAAATTAGGATGAACG+GGG + chr4_2:65839056-65839075 Msa0616140:intron 40.0%
! GACATCAGCTTACACTTTTG+TGG - chr4_2:65839159-65839178 None:intergenic 40.0%
GCTGATGTCAATTGAGCATT+TGG + chr4_2:65839170-65839189 Msa0616140:intron 40.0%
TGACTCTAGCATACTCACTA+TGG - chr4_2:65839698-65839717 None:intergenic 40.0%
GAGTCAATCCTAGAGATTGT+TGG + chr4_2:65839712-65839731 Msa0616140:CDS 40.0%
TAAAGAAACTCACCGGATGA+CGG - chr4_2:65839779-65839798 None:intergenic 40.0%
! TTTTACATGCAGCCTCTTGA+CGG + chr4_2:65839907-65839926 Msa0616140:intron 40.0%
!! GCTATTTTACTGCCATTGGT+AGG - chr4_2:65840120-65840139 None:intergenic 40.0%
TGGCAGTAAAATAGCTACAC+AGG + chr4_2:65840125-65840144 Msa0616140:CDS 40.0%
GGCAGTAAAATAGCTACACA+GGG + chr4_2:65840126-65840145 Msa0616140:CDS 40.0%
GCAGTAAAATAGCTACACAG+GGG + chr4_2:65840127-65840146 Msa0616140:CDS 40.0%
TCAATGACAATGACCAACCA+AGG + chr4_2:65840150-65840169 Msa0616140:CDS 40.0%
AACTTGGATTCTCAGTTCCT+TGG - chr4_2:65840170-65840189 None:intergenic 40.0%
!! TGTTTTTCAATGCCGCCAAA+AGG - chr4_2:65840201-65840220 None:intergenic 40.0%
AACACCTGCAACATTTGCTT+GGG - chr4_2:65840244-65840263 None:intergenic 40.0%
!! GCAGTCTCAAATTTTTGGAC+AGG - chr4_2:65840276-65840295 None:intergenic 40.0%
! GTGGAGCAGTCTCAAATTTT+TGG - chr4_2:65840281-65840300 None:intergenic 40.0%
TCATTATCTAACCCTTCAGG+TGG - chr4_2:65840342-65840361 None:intergenic 40.0%
ACCTGAAGGGTTAGATAATG+AGG + chr4_2:65840341-65840360 Msa0616140:CDS 40.0%
ACAAGAGGTCAAGATGAAAG+AGG + chr4_2:65840582-65840601 Msa0616140:CDS 40.0%
TTTCTAGGTTCCCAACCATA+TGG - chr4_2:65840729-65840748 None:intergenic 40.0%
ATATGGTTGGGAACCTAGAA+AGG + chr4_2:65840728-65840747 Msa0616140:CDS 40.0%
TATGGTTGGGAACCTAGAAA+GGG + chr4_2:65840729-65840748 Msa0616140:CDS 40.0%
GATCAGCCCAAATAGAAAGA+AGG + chr4_2:65840751-65840770 Msa0616140:CDS 40.0%
!!! AAGTCATCGATTTTCTCGGA+AGG + chr4_2:65840890-65840909 Msa0616140:CDS 40.0%
ATCTCTCATTTGAAGGCAAC+TGG - chr4_2:65840923-65840942 None:intergenic 40.0%
! TTGACGGATCTCTCATTTGA+AGG - chr4_2:65840930-65840949 None:intergenic 40.0%
GTGGAAGATTTATTCTGCCT+CGG - chr4_2:65840962-65840981 None:intergenic 40.0%
AGGATCCAGGATAGATTAAG+AGG + chr4_2:65841116-65841135 Msa0616140:CDS 40.0%
TCCAGGATAGATTAAGAGGA+AGG + chr4_2:65841120-65841139 Msa0616140:CDS 40.0%
ACCTTCCTCTTAATCTATCC+TGG - chr4_2:65841124-65841143 None:intergenic 40.0%
TTGCTGAACTTAAGTATGGG+AGG + chr4_2:65841219-65841238 Msa0616140:CDS 40.0%
TTGGAAAGAGGAAAAGCTTC+AGG + chr4_2:65841270-65841289 Msa0616140:CDS 40.0%
TGGAAAGAGGAAAAGCTTCA+GGG + chr4_2:65841271-65841290 Msa0616140:CDS 40.0%
!!! AGAGGTTTTGGACCTTCAAA+TGG - chr4_2:65841323-65841342 None:intergenic 40.0%
! GTCATCATGTTTGCTACTGT+GGG - chr4_2:65841396-65841415 None:intergenic 40.0%
! TGTCATCATGTTTGCTACTG+TGG - chr4_2:65841397-65841416 None:intergenic 40.0%
! CTCTGACAATGTTCCGTTTT+CGG - chr4_2:65841438-65841457 None:intergenic 40.0%
ACCTTCATACTCCTGATCTT+CGG - chr4_2:65841564-65841583 None:intergenic 40.0%
GGAGACTATGAATATGAGCA+AGG + chr4_2:65841587-65841606 Msa0616140:CDS 40.0%
TGAATATGAGCAAGGTGATG+AGG + chr4_2:65841595-65841614 Msa0616140:CDS 40.0%
GATGACTTTGCCAAGAAGAT+TGG + chr4_2:65841683-65841702 Msa0616140:CDS 40.0%
CTCTCTCTACCCAATTTCTG+TGG + chr4_2:65838453-65838472 Msa0616140:five_prime_UTR 45.0%
TCTCTACCCAATTTCTGTGG+CGG + chr4_2:65838456-65838475 Msa0616140:five_prime_UTR 45.0%
GAGAAGAGGAGAAGAAGTAG+AGG - chr4_2:65838493-65838512 None:intergenic 45.0%
GATCCATCGATCTCTTCTTC+TGG + chr4_2:65838526-65838545 Msa0616140:five_prime_UTR 45.0%
AACCCAGAAGAAGAGATCGA+TGG - chr4_2:65838532-65838551 None:intergenic 45.0%
ATCGATCTCTTCTTCTGGGT+TGG + chr4_2:65838531-65838550 Msa0616140:five_prime_UTR 45.0%
TCGATCTCTTCTTCTGGGTT+GGG + chr4_2:65838532-65838551 Msa0616140:five_prime_UTR 45.0%
! GAATCTGAAGTTGTGGCTGT+TGG - chr4_2:65838575-65838594 None:intergenic 45.0%
! TCATCTTGAGGAGAAGGTGT+TGG - chr4_2:65838609-65838628 None:intergenic 45.0%
AAGGCTTCATCTTGAGGAGA+AGG - chr4_2:65838615-65838634 None:intergenic 45.0%
CAGAGTGGGTGAAGGAAATT+AGG + chr4_2:65839046-65839065 Msa0616140:intron 45.0%
TTGTGGCTGTGCAAATCATG+TGG + chr4_2:65839516-65839535 Msa0616140:intron 45.0%
CTCTAGCATACTCACTATGG+CGG - chr4_2:65839695-65839714 None:intergenic 45.0%
!! GGATGACGGAATGAACACTT+GGG - chr4_2:65839765-65839784 None:intergenic 45.0%
CAAGTGTTCATTCCGTCATC+CGG + chr4_2:65839764-65839783 Msa0616140:CDS 45.0%
! GTGCTGGTAAAGAAACTCAC+CGG - chr4_2:65839786-65839805 None:intergenic 45.0%
AAAGGACAGAAAACTCGTGC+TGG - chr4_2:65839802-65839821 None:intergenic 45.0%
TTTACATGCAGCCTCTTGAC+GGG + chr4_2:65839908-65839927 Msa0616140:intron 45.0%
ACCATTATAAGGCGGTGCAT+GGG - chr4_2:65840013-65840032 None:intergenic 45.0%
TACCATTATAAGGCGGTGCA+TGG - chr4_2:65840014-65840033 None:intergenic 45.0%
TTACTGCCATTGGTAGGGTT+TGG - chr4_2:65840114-65840133 None:intergenic 45.0%
TGGATTCTCAGTTCCTTGGT+TGG - chr4_2:65840166-65840185 None:intergenic 45.0%
ACTTCCCAAGCAAATGTTGC+AGG + chr4_2:65840237-65840256 Msa0616140:CDS 45.0%
CAACACCTGCAACATTTGCT+TGG - chr4_2:65840245-65840264 None:intergenic 45.0%
TTATCTAACCCTTCAGGTGG+TGG - chr4_2:65840339-65840358 None:intergenic 45.0%
GCCTCATTATCTAACCCTTC+AGG - chr4_2:65840345-65840364 None:intergenic 45.0%
TTGATGCACACGATTAGACC+GGG - chr4_2:65840418-65840437 None:intergenic 45.0%
GTTGATGCACACGATTAGAC+CGG - chr4_2:65840419-65840438 None:intergenic 45.0%
TTGTGATCATCTGGCAAACG+AGG - chr4_2:65840441-65840460 None:intergenic 45.0%
CGTCATTGTCCTTACCACTA+TGG - chr4_2:65840467-65840486 None:intergenic 45.0%
GCTACAAGAACATCAAACCC+AGG - chr4_2:65840510-65840529 None:intergenic 45.0%
GATCACATGCAAGAGCCATA+TGG + chr4_2:65840711-65840730 Msa0616140:CDS 45.0%
ACATGCAAGAGCCATATGGT+TGG + chr4_2:65840715-65840734 Msa0616140:CDS 45.0%
CATGCAAGAGCCATATGGTT+GGG + chr4_2:65840716-65840735 Msa0616140:CDS 45.0%
ATTTGGGCTGATCCCTTTCT+AGG - chr4_2:65840744-65840763 None:intergenic 45.0%
TGCGCTTGGATAAACGATGT+CGG - chr4_2:65840824-65840843 None:intergenic 45.0%
TCCGTCAATAATCGTTTCCG+AGG + chr4_2:65840942-65840961 Msa0616140:CDS 45.0%
GCCTCGGAAACGATTATTGA+CGG - chr4_2:65840946-65840965 None:intergenic 45.0%
TTGGACAGAGGAAGAATCAG+TGG + chr4_2:65841053-65841072 Msa0616140:CDS 45.0%
AAGTTTCTCCTCTCGTCATC+TGG - chr4_2:65841149-65841168 None:intergenic 45.0%
CTGAAGGAGAAGAAAGGAGT+TGG + chr4_2:65841353-65841372 Msa0616140:CDS 45.0%
! ATCATGTTTGCTACTGTGGG+AGG - chr4_2:65841393-65841412 None:intergenic 45.0%
GGAAGAAGAGTCCAAGATTC+AGG + chr4_2:65841463-65841482 Msa0616140:CDS 45.0%
AACTCACGGTCAATCATCTG+AGG + chr4_2:65841514-65841533 Msa0616140:CDS 45.0%
! GATCAGGAGTATGAAGGTGA+AGG + chr4_2:65841566-65841585 Msa0616140:CDS 45.0%
GACAGATATTACCCCAGTTG+AGG - chr4_2:65841830-65841849 None:intergenic 45.0%
!!! AAAAATTTAATTTTGATTAA+TGG + chr4_2:65838813-65838832 Msa0616140:intron 5.0%
!!! AAAATTTAATTTTGATTAAT+GGG + chr4_2:65838814-65838833 Msa0616140:intron 5.0%
!! TAATAAAATAACAAAAATTA+TGG - chr4_2:65839111-65839130 None:intergenic 5.0%
!!! CTTTTTATTTTAATAATAAT+AGG - chr4_2:65839322-65839341 None:intergenic 5.0%
TGGGTAGAGAGAGAGAGTAG+AGG - chr4_2:65838446-65838465 None:intergenic 50.0%
AGAGAGCCGCCACAGAAATT+GGG - chr4_2:65838465-65838484 None:intergenic 50.0%
!! CGGATGACGGAATGAACACT+TGG - chr4_2:65839766-65839785 None:intergenic 50.0%
GTTCCTAACAACCCGTCAAG+AGG - chr4_2:65839922-65839941 None:intergenic 50.0%
ACCCATGCACCGCCTTATAA+TGG + chr4_2:65840009-65840028 Msa0616140:CDS 50.0%
CATGGACCAAACCCTACCAA+TGG + chr4_2:65840105-65840124 Msa0616140:CDS 50.0%
ACACGATTAGACCGGGCATT+AGG - chr4_2:65840411-65840430 None:intergenic 50.0%
TTTCTCAGAGAGTCGTCTCC+TGG + chr4_2:65840489-65840508 Msa0616140:CDS 50.0%
TTCTCAGAGAGTCGTCTCCT+GGG + chr4_2:65840490-65840509 Msa0616140:CDS 50.0%
GATAGATTTCGTGTGCCCCA+AGG + chr4_2:65840675-65840694 Msa0616140:CDS 50.0%
TCGTAGTCCTGACAGTGTTG+AGG + chr4_2:65840788-65840807 Msa0616140:CDS 50.0%
! TCTGAGACCTCAACACTGTC+AGG - chr4_2:65840798-65840817 None:intergenic 50.0%
GACATCGTTTATCCAAGCGC+AGG + chr4_2:65840823-65840842 Msa0616140:CDS 50.0%
TCGTTTATCCAAGCGCAGGA+AGG + chr4_2:65840827-65840846 Msa0616140:CDS 50.0%
CGTTTATCCAAGCGCAGGAA+GGG + chr4_2:65840828-65840847 Msa0616140:CDS 50.0%
ATCTGTCGTCGCTCATGATG+AGG + chr4_2:65840863-65840882 Msa0616140:CDS 50.0%
TGACTGTCACCACCATTTGG+TGG - chr4_2:65840981-65841000 None:intergenic 50.0%
AGGTGACTGTCACCACCATT+TGG - chr4_2:65840984-65841003 None:intergenic 50.0%
TGGTGACAGTCACCTAGAGA+GGG + chr4_2:65840989-65841008 Msa0616140:CDS 50.0%
CTCTGTCTAAGTCCCTCTCT+AGG - chr4_2:65841004-65841023 None:intergenic 50.0%
CTAGAGAGGGACTTAGACAG+AGG + chr4_2:65841002-65841021 Msa0616140:CDS 50.0%
AACCAGCAGTCACCTAGAGA+GGG + chr4_2:65841028-65841047 Msa0616140:CDS 50.0%
ACAGAGGAAGAATCAGTGGC+CGG + chr4_2:65841057-65841076 Msa0616140:CDS 50.0%
GAAGCCTTCCAGATGACAAC+CGG - chr4_2:65841079-65841098 None:intergenic 50.0%
AAGGCTTCAATCGCCTCATG+AGG + chr4_2:65841091-65841110 Msa0616140:CDS 50.0%
AGGCTTCAATCGCCTCATGA+GGG + chr4_2:65841092-65841111 Msa0616140:CDS 50.0%
TTCAATCGCCTCATGAGGGA+AGG + chr4_2:65841096-65841115 Msa0616140:CDS 50.0%
CTTCAGCAGTCGATCGAATG+AGG + chr4_2:65841166-65841185 Msa0616140:CDS 50.0%
GATCGAATGAGGACCGAAGT+GGG + chr4_2:65841177-65841196 Msa0616140:CDS 50.0%
GAGAAGAAAGGAGTTGGAGC+TGG + chr4_2:65841359-65841378 Msa0616140:CDS 50.0%
CGGAACATTGTCAGAGAGCA+AGG + chr4_2:65841442-65841461 Msa0616140:CDS 50.0%
TCCAAGATTCAGGCTGCTGA+TGG + chr4_2:65841473-65841492 Msa0616140:CDS 50.0%
TCCATCAGCAGCCTGAATCT+TGG - chr4_2:65841477-65841496 None:intergenic 50.0%
CACGGTCAATCATCTGAGGA+AGG + chr4_2:65841518-65841537 Msa0616140:CDS 50.0%
! GCCGAAGATCAGGAGTATGA+AGG + chr4_2:65841560-65841579 Msa0616140:CDS 50.0%
TTAAGCATGCAGGCCTCAAC+TGG + chr4_2:65841814-65841833 Msa0616140:three_prime_UTR 50.0%
TAAGCATGCAGGCCTCAACT+GGG + chr4_2:65841815-65841834 Msa0616140:three_prime_UTR 50.0%
GAGAGAGCCGCCACAGAAAT+TGG - chr4_2:65838466-65838485 None:intergenic 55.0%
TTGTGGCTGTTGGGCAACCA+TGG - chr4_2:65838565-65838584 None:intergenic 55.0%
!! CAGCCTCTTGACGGGTTGTT+AGG + chr4_2:65839916-65839935 Msa0616140:intron 55.0%
CGGGTTGTTAGGAACACCAC+CGG + chr4_2:65839927-65839946 Msa0616140:CDS 55.0%
GCAGATGTTGCCGCAATCTG+TGG - chr4_2:65839988-65840007 None:intergenic 55.0%
GACAGATGTGCCTTCCTGCA+TGG + chr4_2:65840087-65840106 Msa0616140:CDS 55.0%
! AGGGTTTGGTCCATGCAGGA+AGG - chr4_2:65840100-65840119 None:intergenic 55.0%
! TGGTAGGGTTTGGTCCATGC+AGG - chr4_2:65840104-65840123 None:intergenic 55.0%
ATGTACCGCCACCACCTGAA+GGG + chr4_2:65840328-65840347 Msa0616140:CDS 55.0%
! TCTAACCCTTCAGGTGGTGG+CGG - chr4_2:65840336-65840355 None:intergenic 55.0%
ATTGGTTGGCGGTGGAGAAC+TGG - chr4_2:65840385-65840404 None:intergenic 55.0%
AGTTCTCCACCGCCAACCAA+TGG + chr4_2:65840384-65840403 Msa0616140:CDS 55.0%
GTTCTCCACCGCCAACCAAT+GGG + chr4_2:65840385-65840404 Msa0616140:CDS 55.0%
! GCATTAGGCCCATTGGTTGG+CGG - chr4_2:65840396-65840415 None:intergenic 55.0%
CAACCAATGGGCCTAATGCC+CGG + chr4_2:65840397-65840416 Msa0616140:CDS 55.0%
TCCAAGCGCAGGAAGGGAAA+CGG + chr4_2:65840834-65840853 Msa0616140:CDS 55.0%
GTGGTGACAGTCACCTAGAG+AGG + chr4_2:65840988-65841007 Msa0616140:CDS 55.0%
GAACCAGCAGTCACCTAGAG+AGG + chr4_2:65841027-65841046 Msa0616140:CDS 55.0%
CAGTCACCTAGAGAGGGACT+TGG + chr4_2:65840995-65841014 Msa0616140:CDS 55.0%
CTCTGTCCAAGTCCCTCTCT+AGG - chr4_2:65841043-65841062 None:intergenic 55.0%
CTAGAGAGGGACTTGGACAG+AGG + chr4_2:65841041-65841060 Msa0616140:CDS 55.0%
ATCAGTGGCCGGTTGTCATC+TGG + chr4_2:65841068-65841087 Msa0616140:CDS 55.0%
TCCTGGATCCTTCCCTCATG+AGG - chr4_2:65841107-65841126 None:intergenic 55.0%
GAAGGTTGCCAGATGACGAG+AGG + chr4_2:65841138-65841157 Msa0616140:CDS 55.0%
CGATCGAATGAGGACCGAAG+TGG + chr4_2:65841176-65841195 Msa0616140:CDS 55.0%
TTGGCGCAGAGAACCCACTT+CGG - chr4_2:65841193-65841212 None:intergenic 55.0%
AAAGGAGTTGGAGCTGGTGC+AGG + chr4_2:65841365-65841384 Msa0616140:CDS 55.0%
CGATGAAGCTGCCGAAGATC+AGG + chr4_2:65841550-65841569 Msa0616140:CDS 55.0%
AAGCATGCAGGCCTCAACTG+GGG + chr4_2:65841816-65841835 Msa0616140:three_prime_UTR 55.0%
! CTGGGTTGGGTTGTTCGCCA+TGG + chr4_2:65838545-65838564 Msa0616140:exon 60.0%
ATCTGTGGCGTGGGGACAGA+TGG - chr4_2:65839973-65839992 None:intergenic 60.0%
TGTCCCCACGCCACAGATTG+CGG + chr4_2:65839975-65839994 Msa0616140:CDS 60.0%
TTGCCGCAATCTGTGGCGTG+GGG - chr4_2:65839981-65840000 None:intergenic 60.0%
GTTGCCGCAATCTGTGGCGT+GGG - chr4_2:65839982-65840001 None:intergenic 60.0%
TGTTGCCGCAATCTGTGGCG+TGG - chr4_2:65839983-65840002 None:intergenic 60.0%
CATGTACCGCCACCACCTGA+AGG + chr4_2:65840327-65840346 Msa0616140:CDS 60.0%
! TTAGGCCCATTGGTTGGCGG+TGG - chr4_2:65840393-65840412 None:intergenic 60.0%
CGGGCATTAGGCCCATTGGT+TGG - chr4_2:65840399-65840418 None:intergenic 60.0%
AGACCGGGCATTAGGCCCAT+TGG - chr4_2:65840403-65840422 None:intergenic 60.0%
GTCCCTCTCTAGGTGACTGC+TGG - chr4_2:65841033-65841052 None:intergenic 60.0%
GTGGCCGGTTGTCATCTGGA+AGG + chr4_2:65841072-65841091 Msa0616140:CDS 60.0%
GCCTCATGAGGGAAGGATCC+AGG + chr4_2:65841103-65841122 Msa0616140:CDS 60.0%
CCAGCAGCAGCAGCAGTTGC+CGG - chr4_2:65839949-65839968 None:intergenic 65.0%
GCCGTTTCCCTTCCTGCGCT+TGG - chr4_2:65840838-65840857 None:intergenic 65.0%
GCAGCAGCAGCAGTTGCCGG+TGG - chr4_2:65839946-65839965 None:intergenic 70.0%
CCGGCAACTGCTGCTGCTGC+TGG + chr4_2:65839946-65839965 Msa0616140:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 65838291 65842045 65838291 ID=Msa0616140;Name=Msa0616140
chr4_2 mRNA 65838291 65842045 65838291 ID=Msa0616140-mRNA-1;Parent=Msa0616140;Name=Msa0616140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=273|1|1|1|1|1|3|324|679
chr4_2 exon 65838291 65838689 65838291 ID=Msa0616140-mRNA-1:exon:18669;Parent=Msa0616140-mRNA-1
chr4_2 exon 65839675 65839785 65839675 ID=Msa0616140-mRNA-1:exon:18670;Parent=Msa0616140-mRNA-1
chr4_2 exon 65839919 65842045 65839919 ID=Msa0616140-mRNA-1:exon:18671;Parent=Msa0616140-mRNA-1
chr4_2 five_prime_UTR 65838291 65838563 65838291 ID=Msa0616140-mRNA-1:five_prime_utr;Parent=Msa0616140-mRNA-1
chr4_2 CDS 65838564 65838689 65838564 ID=Msa0616140-mRNA-1:cds;Parent=Msa0616140-mRNA-1
chr4_2 CDS 65839675 65839785 65839675 ID=Msa0616140-mRNA-1:cds;Parent=Msa0616140-mRNA-1
chr4_2 CDS 65839919 65841721 65839919 ID=Msa0616140-mRNA-1:cds;Parent=Msa0616140-mRNA-1
chr4_2 three_prime_UTR 65841722 65842045 65841722 ID=Msa0616140-mRNA-1:three_prime_utr;Parent=Msa0616140-mRNA-1
Gene Sequence

>Msa0616140

ATGGTTGCCCAACAGCCACAACTTCAGATTCAGACTCAGCAACCAACACCTTCTCCTCAAGATGAAGCCTTGAAAAGGAACACCGATTGTGTCTACTTTCTTGCTTCTCCCTTGACATGCAAAAAGGGAAACGAATGTGAGTACCGCCATAGTGAGTATGCTAGAGTCAATCCTAGAGATTGTTGGTATTGGTTGAATGGTAACTGCTTGAATCCCAAGTGTTCATTCCGTCATCCGCCTCTTGACGGGTTGTTAGGAACACCACCGGCAACTGCTGCTGCTGCTGGACCATCTGTCCCCACGCCACAGATTGCGGCAACATCTGCAACCCATGCACCGCCTTATAATGGTAGTAAACAAGCCGTGCCTTGCATTTTCTTTCAAAAGGGGTTTTGCTTAAAAGGTGACAGATGTGCCTTCCTGCATGGACCAAACCCTACCAATGGCAGTAAAATAGCTACACAGGGGTCAATGACAATGACCAACCAAGGAACTGAGAATCCAAGTTTTAAGAAACCTTTTGGCGGCATTGAAAAACATGCACAAGAAAGGAAAACTTCCCAAGCAAATGTTGCAGGTGTTGAAGCTAAACCTGTCCAAAAATTTGAGACTGCTCCACAGAAAAATATGTTTAAGTTGGTGAAGCATGTACCGCCACCACCTGAAGGGTTAGATAATGAGGCTTCGCGATTTAAGACAACCAGTTCTCCACCGCCAACCAATGGGCCTAATGCCCGGTCTAATCGTGTGCATCAACCTCGTTTGCCAGATGATCACAATTTCCATAGTGGTAAGGACAATGACGAGTTTCTCAGAGAGTCGTCTCCTGGGTTTGATGTTCTTGTAGCTGACGAACTTAGAAATTCTGATTACTATCATGGGGAAGATGAATTTGGTAAAACAAGAGGTCAAGATGAAAGAGGCCCAGACTCTTTGAATGAATACGATGTAGGGCATTCTGCTGATTATAGTTTAGCTGCTGATATTGATCGAGATAGATTTCGTGTGCCCCAAGGTTATGACTCATATGATCACATGCAAGAGCCATATGGTTGGGAACCTAGAAAGGGATCAGCCCAAATAGAAAGAAGGACTCATCACAGATCTCGTAGTCCTGACAGTGTTGAGGTCTCAGATCTCCGACATCGTTTATCCAAGCGCAGGAAGGGAAACGGCTTAAAATCTGTCGTCGCTCATGATGAGGAGCAAAGTCATCGATTTTCTCGGAAGGATTCACACCAGTTGCCTTCAAATGAGAGATCCGTCAATAATCGTTTCCGAGGCAGAATAAATCTTCCACCAAATGGTGGTGACAGTCACCTAGAGAGGGACTTAGACAGAGGAAGAACCAGCAGTCACCTAGAGAGGGACTTGGACAGAGGAAGAATCAGTGGCCGGTTGTCATCTGGAAGGCTTCAATCGCCTCATGAGGGAAGGATCCAGGATAGATTAAGAGGAAGGTTGCCAGATGACGAGAGGAGAAACTTCAGCAGTCGATCGAATGAGGACCGAAGTGGGTTCTCTGCGCCAAAAAGTCTTGCTGAACTTAAGTATGGGAGGAACACTGAGAATAATGACCAACAATCATTTGGAAAGAGGAAAAGCTTCAGGGATCACCAACAATATGAAGATGATGTACCATTTGAAGGTCCAAAACCTCTTAGTGAAATTCTGAAGGAGAAGAAAGGAGTTGGAGCTGGTGCAGGCTCCTCCCACAGTAGCAAACATGATGACAATAAGAATGAAGGAATTACCGAAAACGGAACATTGTCAGAGAGCAAGGAAGAAGAGTCCAAGATTCAGGCTGCTGATGGAGTTGACAATACTGATGTAACTCACGGTCAATCATCTGAGGAAGGAATGATATACGATGAAGCTGCCGAAGATCAGGAGTATGAAGGTGAAGGAGACTATGAATATGAGCAAGGTGATGAGGAGTATGAGTATGAACAAGTTGAGGGTGAAAATCAAGAGCAAGAATATATGGAAGATGAAGATGGGGATGACTTTGCCAAGAAGATTGGTGTTGTTCTTTCATAA
Protein sequence

>Msa0616140

MVAQQPQLQIQTQQPTPSPQDEALKRNTDCVYFLASPLTCKKGNECEYRHSEYARVNPRDCWYWLNGNCLNPKCSFRHPPLDGLLGTPPATAAAAGPSVPTPQIAATSATHAPPYNGSKQAVPCIFFQKGFCLKGDRCAFLHGPNPTNGSKIATQGSMTMTNQGTENPSFKKPFGGIEKHAQERKTSQANVAGVEAKPVQKFETAPQKNMFKLVKHVPPPPEGLDNEASRFKTTSSPPPTNGPNARSNRVHQPRLPDDHNFHSGKDNDEFLRESSPGFDVLVADELRNSDYYHGEDEFGKTRGQDERGPDSLNEYDVGHSADYSLAADIDRDRFRVPQGYDSYDHMQEPYGWEPRKGSAQIERRTHHRSRSPDSVEVSDLRHRLSKRRKGNGLKSVVAHDEEQSHRFSRKDSHQLPSNERSVNNRFRGRINLPPNGGDSHLERDLDRGRTSSHLERDLDRGRISGRLSSGRLQSPHEGRIQDRLRGRLPDDERRNFSSRSNEDRSGFSAPKSLAELKYGRNTENNDQQSFGKRKSFRDHQQYEDDVPFEGPKPLSEILKEKKGVGAGAGSSHSSKHDDNKNEGITENGTLSESKEEESKIQAADGVDNTDVTHGQSSEEGMIYDEAAEDQEYEGEGDYEYEQGDEEYEYEQVEGENQEQEYMEDEDGDDFAKKIGVVLS*