AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0616650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAGATGTTGATTGAAAT+TGG 0.232139 4_2:-66585386 None:intergenic
GCTGATTATTGGTGCTGTTC+TGG 0.274728 4_2:+66590131 Msa0616650:CDS
GTGTGCTATATGCTGATTAT+TGG 0.276432 4_2:+66590120 Msa0616650:exon
AAGGAGAGGAGCACGGAAAC+TGG 0.341508 4_2:+66585513 Msa0616650:five_prime_UTR
GTTTCCGTGCTCCTCTCCTT+TGG 0.345017 4_2:-66585510 None:intergenic
GAAGTGGCGAATGCTGGATT+CGG 0.369146 4_2:-66585364 None:intergenic
TACCTCTTCCTTCTCAGCTT+CGG 0.371599 4_2:-66585595 None:intergenic
TGCACAGCAGTTGGTGTGTT+GGG 0.380180 4_2:+66590266 Msa0616650:CDS
AAGTGGCGAATGCTGGATTC+GGG 0.404934 4_2:-66585363 None:intergenic
GGAGATAAGAGTGGTTGCGG+TGG 0.408593 4_2:+66590082 Msa0616650:five_prime_UTR
CTGGGCGTGTGTGTTGCTGC+TGG 0.421015 4_2:+66590212 Msa0616650:CDS
GATAAGAGTGGTTGCGGTGG+GGG 0.425767 4_2:+66590085 Msa0616650:five_prime_UTR
TGTGTGTTGCTGCTGGTGCT+TGG 0.431722 4_2:+66590219 Msa0616650:CDS
TTCGTTCCATCAGTTCGAAA+TGG 0.443647 4_2:-66585446 None:intergenic
GTCATGTTGCTGCAGCCTGC+AGG 0.444143 4_2:+66590168 Msa0616650:CDS
GAGATAAGAGTGGTTGCGGT+GGG 0.449712 4_2:+66590083 Msa0616650:five_prime_UTR
CGCAAGCCATTTCGAACTGA+TGG 0.458753 4_2:+66585440 Msa0616650:five_prime_UTR
AAATTGGAAGTGGCGAATGC+TGG 0.474286 4_2:-66585370 None:intergenic
TCTCCTTTGGATCGAACAAA+AGG 0.474957 4_2:-66585497 None:intergenic
TTGCACAGCAGTTGGTGTGT+TGG 0.496637 4_2:+66590265 Msa0616650:CDS
GATTATTGGTGCTGTTCTGG+TGG 0.500980 4_2:+66590134 Msa0616650:CDS
GAGCAGATCCGAAGCTGAGA+AGG 0.503469 4_2:+66585587 Msa0616650:five_prime_UTR
GCAGTTGGTTGCACAGCAGT+TGG 0.506350 4_2:+66590257 Msa0616650:CDS
GAGGGAGATAAGAGTGGTTG+CGG 0.506947 4_2:+66590079 Msa0616650:five_prime_UTR
GTTGCTGCAGCCTGCAGGAA+TGG 0.508627 4_2:+66590173 Msa0616650:CDS
CAGTTCAGTACCTCAGCAGT+TGG 0.511945 4_2:+66590242 Msa0616650:CDS
GGTGCTGTTCTGGTGGATTG+TGG 0.552935 4_2:+66590141 Msa0616650:CDS
CAGTAAAACACCATTCCTGC+AGG 0.574412 4_2:-66590183 None:intergenic
ATGTTGATTGAAATTGGAAG+TGG 0.577999 4_2:-66585380 None:intergenic
ATCCGAAGCTGAGAAGGAAG+AGG 0.578030 4_2:+66585593 Msa0616650:five_prime_UTR
GAGGAGCACGGAAACTGGAT+CGG 0.581402 4_2:+66585518 Msa0616650:five_prime_UTR
AGATAAGAGTGGTTGCGGTG+GGG 0.584561 4_2:+66590084 Msa0616650:five_prime_UTR
CGGTGTCTAAGTAGGGAGGG+AGG 0.584662 4_2:+66590060 Msa0616650:intron
GGTGTCTAAGTAGGGAGGGA+GGG 0.592620 4_2:+66590061 Msa0616650:intron
GGGAGGGAGGGAGATAAGAG+TGG 0.594193 4_2:+66590073 Msa0616650:intron
CCTTTGGATCGAACAAAAGG+AGG 0.610982 4_2:-66585494 None:intergenic
CGATCCAAAGGAGAGGAGCA+CGG 0.618840 4_2:+66585506 Msa0616650:five_prime_UTR
TGCTGTGCAACCAACTGCTG+AGG 0.674136 4_2:-66590252 None:intergenic
GGAACGAATCGATCTCAGCA+AGG 0.681192 4_2:+66585461 Msa0616650:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTTAATAAATTTAA+TGG + chr4_2:66588847-66588866 Msa0616650:intron 0.0%
!! AAAATAAAATAAAAAAGTCA+TGG - chr4_2:66586260-66586279 None:intergenic 10.0%
!! CAAAAAAAAAAAAAATTTAG+AGG - chr4_2:66587971-66587990 None:intergenic 10.0%
!! TATTTAAAAGACTATAAATA+GGG + chr4_2:66588918-66588937 Msa0616650:intron 10.0%
!!! AAAAAAAGTGATTTTTATTA+CGG - chr4_2:66589062-66589081 None:intergenic 10.0%
!!! TTTAAATAATTTAGTTTGTT+TGG + chr4_2:66589083-66589102 Msa0616650:intron 10.0%
!!! AAAAAATAGATTTTTTTTAG+AGG - chr4_2:66589178-66589197 None:intergenic 10.0%
!! TTTAAAAAATTTGTAACAAA+CGG + chr4_2:66589263-66589282 Msa0616650:intron 10.0%
!!! CTTATCTTAATAAAATTTTA+AGG - chr4_2:66589562-66589581 None:intergenic 10.0%
!!! ATTATTTTGATTAAAATTGT+AGG + chr4_2:66589787-66589806 Msa0616650:intron 10.0%
!!! AAAGTGATGTAATTTATTTT+GGG + chr4_2:66586561-66586580 Msa0616650:intron 15.0%
!!! AAAAGATTTTTCTAAACTAA+CGG - chr4_2:66586720-66586739 None:intergenic 15.0%
!! GAATTTGATTAAGAAAATAA+TGG - chr4_2:66586819-66586838 None:intergenic 15.0%
!!! CTTACAAAAAATATTTTTGT+GGG - chr4_2:66586982-66587001 None:intergenic 15.0%
!!! TCTTACAAAAAATATTTTTG+TGG - chr4_2:66586983-66587002 None:intergenic 15.0%
!! ATAAAAATAAAGTTAGAAAG+AGG + chr4_2:66587147-66587166 Msa0616650:intron 15.0%
!!! TAAAAAAGTGATTTTTACTA+CGG - chr4_2:66587856-66587875 None:intergenic 15.0%
!!! AATTTAATGGATTTATTTGA+TGG + chr4_2:66588860-66588879 Msa0616650:intron 15.0%
!! CTATTTAAAAGACTATAAAT+AGG + chr4_2:66588917-66588936 Msa0616650:intron 15.0%
!!! TATAAATAGGGATTTAATTT+AGG + chr4_2:66588930-66588949 Msa0616650:intron 15.0%
!!! ATAAATAGGGATTTAATTTA+GGG + chr4_2:66588931-66588950 Msa0616650:intron 15.0%
!!! AAAACGAATTTTAAAATAGA+GGG - chr4_2:66589371-66589390 None:intergenic 15.0%
!!! AAAAACGAATTTTAAAATAG+AGG - chr4_2:66589372-66589391 None:intergenic 15.0%
!!! ATAAAACTTCATTTTTTTCA+AGG - chr4_2:66589416-66589435 None:intergenic 15.0%
!! ATTATAATATGTTAATCTCA+AGG - chr4_2:66589505-66589524 None:intergenic 15.0%
!! TTTATTACAATATATGAAAG+TGG - chr4_2:66589832-66589851 None:intergenic 15.0%
!!! TTATAGTTAACTTTGTTTAA+GGG + chr4_2:66589941-66589960 Msa0616650:intron 15.0%
!!! ATATAGATGTTGATTGAAAT+TGG - chr4_2:66585389-66585408 None:intergenic 20.0%
!!! TATATTTTCGAAAATCAACA+GGG + chr4_2:66585404-66585423 Msa0616650:five_prime_UTR 20.0%
!!! TTGAAGAAAATGAATTTTGT+TGG + chr4_2:66585732-66585751 Msa0616650:intron 20.0%
!! AATTAGATAAAATGCATGAT+AGG - chr4_2:66586065-66586084 None:intergenic 20.0%
!!! ATTAAAGTATTCAAAAAGCT+TGG - chr4_2:66586235-66586254 None:intergenic 20.0%
!! AAAGCAAACAAAATATAATG+AGG - chr4_2:66586459-66586478 None:intergenic 20.0%
!! TATACACACTAAATATCATA+GGG - chr4_2:66586535-66586554 None:intergenic 20.0%
!! TTATACACACTAAATATCAT+AGG - chr4_2:66586536-66586555 None:intergenic 20.0%
!! GATATTTAGTGTGTATAATT+AGG + chr4_2:66586538-66586557 Msa0616650:intron 20.0%
!!! GAAAGTGATGTAATTTATTT+TGG + chr4_2:66586560-66586579 Msa0616650:intron 20.0%
!!! AAAGATTTTTCTAAACTAAC+GGG - chr4_2:66586719-66586738 None:intergenic 20.0%
!!! TTGTTTTTCTTAATAAACCT+TGG + chr4_2:66586772-66586791 Msa0616650:intron 20.0%
!!! AAAATAATGGAAAAATCTCT+TGG - chr4_2:66586806-66586825 None:intergenic 20.0%
!!! TTCATTTTATTTGCGAAATT+TGG - chr4_2:66586868-66586887 None:intergenic 20.0%
!!! TTTTGAAAATGATTTTGAGA+AGG - chr4_2:66586938-66586957 None:intergenic 20.0%
!!! AAAAAATATTTTTGTGGGTT+AGG - chr4_2:66586977-66586996 None:intergenic 20.0%
!!! TTTTATTTGATCGATTTGAA+AGG - chr4_2:66587060-66587079 None:intergenic 20.0%
!! TAAAAGAAGTTATAACAATC+AGG + chr4_2:66588122-66588141 Msa0616650:intron 20.0%
!! AACAATGCATAAAATATGTA+AGG - chr4_2:66588386-66588405 None:intergenic 20.0%
!!! TCTTTTAAATAGACTTTCAT+AGG - chr4_2:66588909-66588928 None:intergenic 20.0%
!!! TTATTTTAATCCGTAACAAA+CGG - chr4_2:66588958-66588977 None:intergenic 20.0%
!! CTAAAAAAAGCTATAACAAA+CGG + chr4_2:66589336-66589355 Msa0616650:intron 20.0%
!! TAAAAAAAGCTATAACAAAC+GGG + chr4_2:66589337-66589356 Msa0616650:intron 20.0%
!! TCAAGGAAAATGTTATATAA+AGG - chr4_2:66589399-66589418 None:intergenic 20.0%
!! TATTAAGATAAGAAGAGTTA+AGG + chr4_2:66589570-66589589 Msa0616650:intron 20.0%
!!! CTTATAGTTAACTTTGTTTA+AGG + chr4_2:66589940-66589959 Msa0616650:intron 20.0%
!! CTATATTTTCGAAAATCAAC+AGG + chr4_2:66585403-66585422 Msa0616650:five_prime_UTR 25.0%
! ACACAAACATTAAGATGAAT+GGG - chr4_2:66585642-66585661 None:intergenic 25.0%
!! AAGAAAATGAATTTTGTTGG+TGG + chr4_2:66585735-66585754 Msa0616650:intron 25.0%
!!! ACACATTCATGTGTTTTTTT+TGG + chr4_2:66586023-66586042 Msa0616650:intron 25.0%
!!! TATTACCTTTTAGTTCATGT+AGG + chr4_2:66586119-66586138 Msa0616650:intron 25.0%
!! GATTTATATCCTGCATTTTT+GGG + chr4_2:66586152-66586171 Msa0616650:intron 25.0%
! TATTATGGAGTAAAGATGTA+TGG - chr4_2:66586315-66586334 None:intergenic 25.0%
! CAAATCAACACAAGATATTA+TGG - chr4_2:66586330-66586349 None:intergenic 25.0%
!!! CATAATATCTTGTGTTGATT+TGG + chr4_2:66586328-66586347 Msa0616650:intron 25.0%
!!! CAAAATTTTAAAGCAATGCA+TGG - chr4_2:66586376-66586395 None:intergenic 25.0%
!!! CATGCATTGCTTTAAAATTT+TGG + chr4_2:66586374-66586393 Msa0616650:intron 25.0%
! ACTTAAATGAATCACATACT+AGG - chr4_2:66586427-66586446 None:intergenic 25.0%
! GTGTAAAGCAATGAATAAAA+TGG - chr4_2:66586664-66586683 None:intergenic 25.0%
! AGTTCAATTAGAGTTCATTT+AGG - chr4_2:66586841-66586860 None:intergenic 25.0%
!!! TTTTTTGTAAGAGTGAACTA+CGG + chr4_2:66586991-66587010 Msa0616650:intron 25.0%
! TAAAAGTTGAAGTGAAAGAA+TGG - chr4_2:66587112-66587131 None:intergenic 25.0%
!! GTTTCTATGATTATTAGTCT+AGG + chr4_2:66587424-66587443 Msa0616650:intron 25.0%
!! ATTTTTCTAATGTTGCAGTT+TGG + chr4_2:66587466-66587485 Msa0616650:intron 25.0%
!! ATACATGTTTTCTTATGAGT+TGG - chr4_2:66587722-66587741 None:intergenic 25.0%
! CTACAAAATTTGTGCTAAAA+TGG + chr4_2:66588147-66588166 Msa0616650:intron 25.0%
! CAAACAAACAAAATCCAAAA+CGG - chr4_2:66588232-66588251 None:intergenic 25.0%
! GCAAAAGTAGAAGTTTAAAT+TGG - chr4_2:66588320-66588339 None:intergenic 25.0%
!!! TTTGCCTTTGCAATTTTTTT+TGG + chr4_2:66588335-66588354 Msa0616650:intron 25.0%
!!! CATTTTTGGTTTCTTTTACT+TGG - chr4_2:66588449-66588468 None:intergenic 25.0%
! ATTGCATATTATGTGCATAT+GGG - chr4_2:66588569-66588588 None:intergenic 25.0%
! TATTGCATATTATGTGCATA+TGG - chr4_2:66588570-66588589 None:intergenic 25.0%
! GAGTGATAATATTTGTATCT+CGG - chr4_2:66588617-66588636 None:intergenic 25.0%
! AAATAAAAAATTCCTCCGTA+TGG - chr4_2:66588720-66588739 None:intergenic 25.0%
!!! AAAATTTTGGTGAGTTGTAA+GGG + chr4_2:66588776-66588795 Msa0616650:intron 25.0%
!!! CGTTTGTTATAGCTTTTTTT+AGG - chr4_2:66589338-66589357 None:intergenic 25.0%
! GTTTCATGTTCAATTATTCT+TGG - chr4_2:66589483-66589502 None:intergenic 25.0%
! TTGTTATATTGTTGACATCT+TGG - chr4_2:66589871-66589890 None:intergenic 25.0%
! ATGTTGATTGAAATTGGAAG+TGG - chr4_2:66585383-66585402 None:intergenic 30.0%
ACAAACATTAAGATGAATGG+GGG - chr4_2:66585640-66585659 None:intergenic 30.0%
CACAAACATTAAGATGAATG+GGG - chr4_2:66585641-66585660 None:intergenic 30.0%
CACACAAACATTAAGATGAA+TGG - chr4_2:66585643-66585662 None:intergenic 30.0%
CATTCATCTTAATGTTTGTG+TGG + chr4_2:66585641-66585660 Msa0616650:intron 30.0%
!! GTGGTATTCTTTTTGAAAGT+TGG + chr4_2:66585660-66585679 Msa0616650:intron 30.0%
AACTAGCAAAACATAGAACA+GGG - chr4_2:66585951-66585970 None:intergenic 30.0%
AAACTAGCAAAACATAGAAC+AGG - chr4_2:66585952-66585971 None:intergenic 30.0%
AAAAGCCTACATGAACTAAA+AGG - chr4_2:66586127-66586146 None:intergenic 30.0%
! GGATTTATATCCTGCATTTT+TGG + chr4_2:66586151-66586170 Msa0616650:intron 30.0%
TTGGAACAAAGTTGCAAAAT+AGG - chr4_2:66586216-66586235 None:intergenic 30.0%
!! GGCTTTAAATAAATTGCAAG+AGG - chr4_2:66586514-66586533 None:intergenic 30.0%
ATGGAAAAATCTCTTGGATT+TGG - chr4_2:66586800-66586819 None:intergenic 30.0%
TTTCATTCTTCTATACCTTG+AGG + chr4_2:66586903-66586922 Msa0616650:intron 30.0%
! AGAGATTGAAAAACCCTTTT+TGG - chr4_2:66587240-66587259 None:intergenic 30.0%
! AATGTGTAGACCAAGATTTT+CGG - chr4_2:66587336-66587355 None:intergenic 30.0%
GCAAAAAATCGTATTTGGTT+CGG - chr4_2:66587574-66587593 None:intergenic 30.0%
!!! GAACCAAATACGATTTTTTG+CGG + chr4_2:66587573-66587592 Msa0616650:intron 30.0%
AAACCGCAAAAAATCGTATT+TGG - chr4_2:66587579-66587598 None:intergenic 30.0%
!!! AATACGATTTTTTGCGGTTT+TGG + chr4_2:66587579-66587598 Msa0616650:intron 30.0%
!!! TTGGTTTGCGGTTTTTATAT+TGG + chr4_2:66587613-66587632 Msa0616650:intron 30.0%
GTAGAATTGTGCGTAAAAAT+GGG - chr4_2:66587671-66587690 None:intergenic 30.0%
TTTAGAGGAGAGAGAAAATA+TGG - chr4_2:66587956-66587975 None:intergenic 30.0%
TTCTCTGATTGTTTCGATAA+TGG + chr4_2:66588272-66588291 Msa0616650:intron 30.0%
ACCACCAAAAAAAATTGCAA+AGG - chr4_2:66588342-66588361 None:intergenic 30.0%
!!! TTTGCAATTTTTTTTGGTGG+TGG + chr4_2:66588341-66588360 Msa0616650:intron 30.0%
TGCATAAAATATGTAAGGTC+CGG - chr4_2:66588381-66588400 None:intergenic 30.0%
!!! TTTTTTTGTAAGTAGCCTAG+TGG + chr4_2:66588486-66588505 Msa0616650:intron 30.0%
ACAATATAATTGTGGAGAAG+TGG + chr4_2:66588520-66588539 Msa0616650:intron 30.0%
CAATATAATTGTGGAGAAGT+GGG + chr4_2:66588521-66588540 Msa0616650:intron 30.0%
AATATAATTGTGGAGAAGTG+GGG + chr4_2:66588522-66588541 Msa0616650:intron 30.0%
ATTATCACTCTATCAAAGCA+TGG + chr4_2:66588627-66588646 Msa0616650:intron 30.0%
TCCTCTGTATTTGAATGTTT+GGG - chr4_2:66588696-66588715 None:intergenic 30.0%
ATCCTCTGTATTTGAATGTT+TGG - chr4_2:66588697-66588716 None:intergenic 30.0%
!!! TGTCACAAGTTTTTGATTTG+TGG + chr4_2:66588739-66588758 Msa0616650:intron 30.0%
! AAGCATCTTCTGCAAAATTT+TGG + chr4_2:66588763-66588782 Msa0616650:intron 30.0%
!! CAAAATTTTGGTGAGTTGTA+AGG + chr4_2:66588775-66588794 Msa0616650:intron 30.0%
ACTCAATTAAATATCTCCCT+CGG - chr4_2:66588886-66588905 None:intergenic 30.0%
TTTAGAGGAGAGAGAAAATA+TGG - chr4_2:66587956-66587975 None:intergenic 30.0%
!! TTCTTGGTGTCAATTTGAAT+GGG - chr4_2:66589467-66589486 None:intergenic 30.0%
!! ATTCTTGGTGTCAATTTGAA+TGG - chr4_2:66589468-66589487 None:intergenic 30.0%
TATTGTTGACATCTTGGAAA+AGG - chr4_2:66589865-66589884 None:intergenic 30.0%
AAGATGAATGGGGGAAAAAA+AGG - chr4_2:66585631-66585650 None:intergenic 35.0%
ATAGAGAGAGAGAAGAGAAT+TGG + chr4_2:66585697-66585716 Msa0616650:intron 35.0%
!! TGCTTTTGTATTTGCTGAAC+CGG + chr4_2:66585787-66585806 Msa0616650:intron 35.0%
AAGTAGACAGCAAGCTAAAT+GGG - chr4_2:66585886-66585905 None:intergenic 35.0%
AAAGTAGACAGCAAGCTAAA+TGG - chr4_2:66585887-66585906 None:intergenic 35.0%
ACTAGCAAAACATAGAACAG+GGG - chr4_2:66585950-66585969 None:intergenic 35.0%
! AGTTCATGTAGGCTTTTGAA+AGG + chr4_2:66586130-66586149 Msa0616650:intron 35.0%
TCTTAGTGTGTATCTAGGAA+GGG + chr4_2:66586588-66586607 Msa0616650:intron 35.0%
AGAAGAATGAAATGAGAGTG+AGG - chr4_2:66586895-66586914 None:intergenic 35.0%
!!! GTGGTTTTGATCCCTTTTAA+GGG + chr4_2:66587013-66587032 Msa0616650:intron 35.0%
AGTTGAAGTGAAAGAATGGA+GGG - chr4_2:66587108-66587127 None:intergenic 35.0%
AAGTTGAAGTGAAAGAATGG+AGG - chr4_2:66587109-66587128 None:intergenic 35.0%
AAGTTAGAAAGAGGTTCTCA+CGG + chr4_2:66587156-66587175 Msa0616650:intron 35.0%
! TGAAAAACCCTTTTTGGATC+CGG - chr4_2:66587234-66587253 None:intergenic 35.0%
!!! ACTTGCTCGGTTTTATTCTT+GGG - chr4_2:66587272-66587291 None:intergenic 35.0%
! ATGTGTAGACCAAGATTTTC+GGG - chr4_2:66587335-66587354 None:intergenic 35.0%
TCTAATGTTGCAGTTTGGTT+TGG + chr4_2:66587471-66587490 Msa0616650:intron 35.0%
!!! GATTTTTTGCGGTTTTGGTT+TGG + chr4_2:66587584-66587603 Msa0616650:intron 35.0%
!! TAAAAATGGGTTGATGCTAG+GGG - chr4_2:66587658-66587677 None:intergenic 35.0%
!! GTAAAAATGGGTTGATGCTA+GGG - chr4_2:66587659-66587678 None:intergenic 35.0%
GGTAGAATTGTGCGTAAAAA+TGG - chr4_2:66587672-66587691 None:intergenic 35.0%
! TATGCAGTGATTCTCTTTTC+TGG + chr4_2:66588194-66588213 Msa0616650:intron 35.0%
! ATGCAGTGATTCTCTTTTCT+GGG + chr4_2:66588195-66588214 Msa0616650:intron 35.0%
!!! GCCTTTGCAATTTTTTTTGG+TGG + chr4_2:66588338-66588357 Msa0616650:intron 35.0%
! TTGATAGATCGTCGCATTTT+TGG - chr4_2:66588463-66588482 None:intergenic 35.0%
GAGCTCACACAATATAATTG+TGG + chr4_2:66588512-66588531 Msa0616650:intron 35.0%
ATATTATGTGCATATGGGTC+GGG - chr4_2:66588564-66588583 None:intergenic 35.0%
CATATTATGTGCATATGGGT+CGG - chr4_2:66588565-66588584 None:intergenic 35.0%
CTAGTTAGCATAAGTGACAT+TGG - chr4_2:66588658-66588677 None:intergenic 35.0%
TCCCAAACATTCAAATACAG+AGG + chr4_2:66588692-66588711 Msa0616650:intron 35.0%
TCAAATACAGAGGATCCATA+CGG + chr4_2:66588702-66588721 Msa0616650:intron 35.0%
TGAGTTGTAAGGGATTTAGA+TGG + chr4_2:66588786-66588805 Msa0616650:intron 35.0%
GGGATTTAGATGGAAAAACA+TGG + chr4_2:66588796-66588815 Msa0616650:intron 35.0%
TTAGATGGAAAAACATGGAC+AGG + chr4_2:66588801-66588820 Msa0616650:intron 35.0%
! AATTTAGGGTCCGTTTGTTA+CGG + chr4_2:66588945-66588964 Msa0616650:intron 35.0%
ATTGAAAGTGAAAGTTGTCG+TGG + chr4_2:66589971-66589990 Msa0616650:intron 35.0%
! CTTCTTTTATGAGTGGAGTT+GGG + chr4_2:66590029-66590048 Msa0616650:intron 35.0%
! GTGTGCTATATGCTGATTAT+TGG + chr4_2:66590120-66590139 Msa0616650:exon 35.0%
TTCGTTCCATCAGTTCGAAA+TGG - chr4_2:66585449-66585468 None:intergenic 40.0%
TCTCCTTTGGATCGAACAAA+AGG - chr4_2:66585500-66585519 None:intergenic 40.0%
! TTTTGTTCGATCCAAAGGAG+AGG + chr4_2:66585499-66585518 Msa0616650:five_prime_UTR 40.0%
AGAGAGAAGAGAATTGGTGT+TGG + chr4_2:66585703-66585722 Msa0616650:intron 40.0%
!!! AATGAATTTTGTTGGTGGCG+TGG + chr4_2:66585740-66585759 Msa0616650:intron 40.0%
!!! ATGAATTTTGTTGGTGGCGT+GGG + chr4_2:66585741-66585760 Msa0616650:intron 40.0%
! TTGTATTTGCTGAACCGGTT+CGG + chr4_2:66585792-66585811 Msa0616650:intron 40.0%
GGAGTAAAGAAAAGCTGAAC+CGG - chr4_2:66585840-66585859 None:intergenic 40.0%
TAGCAAAACATAGAACAGGG+GGG - chr4_2:66585948-66585967 None:intergenic 40.0%
CTAGCAAAACATAGAACAGG+GGG - chr4_2:66585949-66585968 None:intergenic 40.0%
TTGAACACACCCAAAAATGC+AGG - chr4_2:66586164-66586183 None:intergenic 40.0%
GCTTCTCTTAGTGTGTATCT+AGG + chr4_2:66586583-66586602 Msa0616650:intron 40.0%
CTCTTAGTGTGTATCTAGGA+AGG + chr4_2:66586587-66586606 Msa0616650:intron 40.0%
! TGAAGTGTCAGTGTTTGTGT+CGG - chr4_2:66586641-66586660 None:intergenic 40.0%
ATCTCTTGGATTTGGATCCA+AGG - chr4_2:66586792-66586811 None:intergenic 40.0%
TTTGTAAGAGTGAACTACGG+TGG + chr4_2:66586994-66587013 Msa0616650:intron 40.0%
!!! GGTGGTTTTGATCCCTTTTA+AGG + chr4_2:66587012-66587031 Msa0616650:intron 40.0%
! AAAAACCCTTTTTGGATCCG+GGG - chr4_2:66587232-66587251 None:intergenic 40.0%
! GAAAAACCCTTTTTGGATCC+GGG - chr4_2:66587233-66587252 None:intergenic 40.0%
!!! CTCGGTTTTATTCTTGGGAA+GGG - chr4_2:66587267-66587286 None:intergenic 40.0%
!!! GACTTGCTCGGTTTTATTCT+TGG - chr4_2:66587273-66587292 None:intergenic 40.0%
AGAATAAAACCGAGCAAGTC+CGG + chr4_2:66587273-66587292 Msa0616650:intron 40.0%
GACTTGAACCTTCAATCTAC+CGG - chr4_2:66587295-66587314 None:intergenic 40.0%
GAAGGTTCAAGTCCAATCAA+TGG + chr4_2:66587302-66587321 Msa0616650:intron 40.0%
!! ATGTTGCAGTTTGGTTTGGT+TGG + chr4_2:66587475-66587494 Msa0616650:intron 40.0%
!! TGTTGCAGTTTGGTTTGGTT+GGG + chr4_2:66587476-66587495 Msa0616650:intron 40.0%
!!! CATTTTCTAGCTCAACCGTA+CGG - chr4_2:66587539-66587558 None:intergenic 40.0%
!!! TTTGCGGTTTTGGTTTGGAT+TGG + chr4_2:66587589-66587608 Msa0616650:intron 40.0%
!!! GGTTTTGGTTTGGATTGGTT+TGG + chr4_2:66587594-66587613 Msa0616650:intron 40.0%
!! GTTTGGATTGGTTTGGTTTG+CGG + chr4_2:66587601-66587620 Msa0616650:intron 40.0%
!! CGTAAAAATGGGTTGATGCT+AGG - chr4_2:66587660-66587679 None:intergenic 40.0%
!!! GGGTTTTATTTTCGCCGTTT+TGG + chr4_2:66588215-66588234 Msa0616650:intron 40.0%
TATTATGTGCATATGGGTCG+GGG - chr4_2:66588563-66588582 None:intergenic 40.0%
! TGAGTTTAGCTCAGTTGGTA+GGG - chr4_2:66588594-66588613 None:intergenic 40.0%
! ATGGATTTATTTGATGGCCG+AGG + chr4_2:66588866-66588885 Msa0616650:intron 40.0%
! TGGATTTATTTGATGGCCGA+GGG + chr4_2:66588867-66588886 Msa0616650:intron 40.0%
! CACTCATAAAAGAAGCACAC+TGG - chr4_2:66590024-66590043 None:intergenic 40.0%
! CAGTGTGCTTCTTTTATGAG+TGG + chr4_2:66590022-66590041 Msa0616650:intron 40.0%
! GCTTCTTTTATGAGTGGAGT+TGG + chr4_2:66590028-66590047 Msa0616650:intron 40.0%
!!! TGGTGTTTTACTGTTTCTGC+TGG + chr4_2:66590193-66590212 Msa0616650:CDS 40.0%
!!! GGTGTTTTACTGTTTCTGCT+GGG + chr4_2:66590194-66590213 Msa0616650:CDS 40.0%
AAATTGGAAGTGGCGAATGC+TGG - chr4_2:66585373-66585392 None:intergenic 45.0%
CCTTTGGATCGAACAAAAGG+AGG - chr4_2:66585497-66585516 None:intergenic 45.0%
! CCTCCTTTTGTTCGATCCAA+AGG + chr4_2:66585494-66585513 Msa0616650:five_prime_UTR 45.0%
TACCTCTTCCTTCTCAGCTT+CGG - chr4_2:66585598-66585617 None:intergenic 45.0%
GTGGGAACAGAGGAATGAAA+CGG + chr4_2:66585759-66585778 Msa0616650:intron 45.0%
TGGGAACAGAGGAATGAAAC+GGG + chr4_2:66585760-66585779 Msa0616650:intron 45.0%
! AAGAAAAGCTGAACCGGTTC+AGG - chr4_2:66585834-66585853 None:intergenic 45.0%
AAGGAGTAAAACTGTGGGCT+TGG - chr4_2:66585861-66585880 None:intergenic 45.0%
! AAGCCCACAGTTTTACTCCT+TGG + chr4_2:66585860-66585879 Msa0616650:intron 45.0%
!! GATCCCTTTTAAGGGTACGT+AGG + chr4_2:66587021-66587040 Msa0616650:intron 45.0%
CTGCCTACGTACCCTTAAAA+GGG - chr4_2:66587027-66587046 None:intergenic 45.0%
TCTGCCTACGTACCCTTAAA+AGG - chr4_2:66587028-66587047 None:intergenic 45.0%
!! TTTTAAGGGTACGTAGGCAG+AGG + chr4_2:66587027-66587046 Msa0616650:intron 45.0%
AGAATGGAGGGAGAAGAAGT+AGG - chr4_2:66587096-66587115 None:intergenic 45.0%
!! TTACGATTCGGGAAGGTGTT+AGG - chr4_2:66587204-66587223 None:intergenic 45.0%
! AAAACCCTTTTTGGATCCGG+GGG - chr4_2:66587231-66587250 None:intergenic 45.0%
!!! GCTCGGTTTTATTCTTGGGA+AGG - chr4_2:66587268-66587287 None:intergenic 45.0%
! TTCAATCTACCGGACTTGCT+CGG - chr4_2:66587285-66587304 None:intergenic 45.0%
!! CAGTTTGGTTTGGTTGGGTT+CGG + chr4_2:66587481-66587500 Msa0616650:intron 45.0%
!! GTACGGTTTGGTTTGGTTTG+CGG - chr4_2:66587522-66587541 None:intergenic 45.0%
GCAAACCAAACCAAACCGTA+CGG + chr4_2:66587521-66587540 Msa0616650:intron 45.0%
TGCAAGTGGAGAAGACACAA+GGG - chr4_2:66587693-66587712 None:intergenic 45.0%
ATGCAAGTGGAGAAGACACA+AGG - chr4_2:66587694-66587713 None:intergenic 45.0%
! TGAGTTGGAGAGAATGCAAG+TGG - chr4_2:66587707-66587726 None:intergenic 45.0%
AAGGTCCGGAGTTCAAACAT+CGG - chr4_2:66588367-66588386 None:intergenic 45.0%
TTGTGTGAGCTCTATCCACT+AGG - chr4_2:66588504-66588523 None:intergenic 45.0%
! GTGAGTTTAGCTCAGTTGGT+AGG - chr4_2:66588595-66588614 None:intergenic 45.0%
AATACAGAGGATCCATACGG+AGG + chr4_2:66588705-66588724 Msa0616650:intron 45.0%
! GTCTCAGAGTTAGTCTAGTG+AGG - chr4_2:66589540-66589559 None:intergenic 45.0%
CACACACACTAGTGTTCGAA+CGG + chr4_2:66589679-66589698 Msa0616650:intron 45.0%
ACACACACTAGTGTTCGAAC+GGG + chr4_2:66589680-66589699 Msa0616650:intron 45.0%
GAAAGTGAAAGTTGTCGTGG+AGG + chr4_2:66589974-66589993 Msa0616650:intron 45.0%
!! ACCCTATTCGGTGTCTAAGT+AGG + chr4_2:66590052-66590071 Msa0616650:intron 45.0%
CCCTACTTAGACACCGAATA+GGG - chr4_2:66590056-66590075 None:intergenic 45.0%
!! CCCTATTCGGTGTCTAAGTA+GGG + chr4_2:66590053-66590072 Msa0616650:intron 45.0%
TCCCTACTTAGACACCGAAT+AGG - chr4_2:66590057-66590076 None:intergenic 45.0%
!! TATTCGGTGTCTAAGTAGGG+AGG + chr4_2:66590056-66590075 Msa0616650:intron 45.0%
!! ATTCGGTGTCTAAGTAGGGA+GGG + chr4_2:66590057-66590076 Msa0616650:intron 45.0%
!! GCTGATTATTGGTGCTGTTC+TGG + chr4_2:66590131-66590150 Msa0616650:CDS 45.0%
!! GATTATTGGTGCTGTTCTGG+TGG + chr4_2:66590134-66590153 Msa0616650:CDS 45.0%
CAGTAAAACACCATTCCTGC+AGG - chr4_2:66590186-66590205 None:intergenic 45.0%
!!! TAATATTCTTATTTAAAATA+AGG + chr4_2:66587741-66587760 Msa0616650:intron 5.0%
!!! AATATTCTTATTTAAAATAA+GGG + chr4_2:66587742-66587761 Msa0616650:intron 5.0%
AAGTGGCGAATGCTGGATTC+GGG - chr4_2:66585366-66585385 None:intergenic 50.0%
GAAGTGGCGAATGCTGGATT+CGG - chr4_2:66585367-66585386 None:intergenic 50.0%
CGCAAGCCATTTCGAACTGA+TGG + chr4_2:66585440-66585459 Msa0616650:five_prime_UTR 50.0%
GGAACGAATCGATCTCAGCA+AGG + chr4_2:66585461-66585480 Msa0616650:five_prime_UTR 50.0%
ATCCGAAGCTGAGAAGGAAG+AGG + chr4_2:66585593-66585612 Msa0616650:five_prime_UTR 50.0%
GGGCCAAGGAGTAAAACTGT+GGG - chr4_2:66585866-66585885 None:intergenic 50.0%
TGGGCCAAGGAGTAAAACTG+TGG - chr4_2:66585867-66585886 None:intergenic 50.0%
ACAGCAAGCTAAATGGGCCA+AGG - chr4_2:66585880-66585899 None:intergenic 50.0%
! TTACACCCTGTGTTGCTGCT+AGG + chr4_2:66585920-66585939 Msa0616650:intron 50.0%
GAGCAAGTCCGGTAGATTGA+AGG + chr4_2:66587284-66587303 Msa0616650:intron 50.0%
TCGGGTGTCAAGCCATTGAT+TGG - chr4_2:66587317-66587336 None:intergenic 50.0%
GGCTTGACACCCGAAAATCT+TGG + chr4_2:66587323-66587342 Msa0616650:intron 50.0%
!! TTGGTTTGGTTGGGTTCGGT+TGG + chr4_2:66587485-66587504 Msa0616650:intron 50.0%
!! CTCAACCGTACGGTTTGGTT+TGG - chr4_2:66587529-66587548 None:intergenic 50.0%
! TCTAGCTCAACCGTACGGTT+TGG - chr4_2:66587534-66587553 None:intergenic 50.0%
! CTCGGTGAGTTTAGCTCAGT+TGG - chr4_2:66588599-66588618 None:intergenic 50.0%
AGTGAAAGTTGTCGTGGAGG+TGG + chr4_2:66589977-66589996 Msa0616650:intron 50.0%
AGTGGAGTTGGGACCCTATT+CGG + chr4_2:66590040-66590059 Msa0616650:intron 50.0%
GAGGGAGATAAGAGTGGTTG+CGG + chr4_2:66590079-66590098 Msa0616650:five_prime_UTR 50.0%
GAGATAAGAGTGGTTGCGGT+GGG + chr4_2:66590083-66590102 Msa0616650:five_prime_UTR 50.0%
AGATAAGAGTGGTTGCGGTG+GGG + chr4_2:66590084-66590103 Msa0616650:five_prime_UTR 50.0%
CAGTTCAGTACCTCAGCAGT+TGG + chr4_2:66590242-66590261 Msa0616650:CDS 50.0%
! TTGCACAGCAGTTGGTGTGT+TGG + chr4_2:66590265-66590284 Msa0616650:CDS 50.0%
! TGCACAGCAGTTGGTGTGTT+GGG + chr4_2:66590266-66590285 Msa0616650:CDS 50.0%
CGATCCAAAGGAGAGGAGCA+CGG + chr4_2:66585506-66585525 Msa0616650:five_prime_UTR 55.0%
GTTTCCGTGCTCCTCTCCTT+TGG - chr4_2:66585513-66585532 None:intergenic 55.0%
! AAGGAGAGGAGCACGGAAAC+TGG + chr4_2:66585513-66585532 Msa0616650:five_prime_UTR 55.0%
! GAGGAGCACGGAAACTGGAT+CGG + chr4_2:66585518-66585537 Msa0616650:five_prime_UTR 55.0%
GAGCAGATCCGAAGCTGAGA+AGG + chr4_2:66585587-66585606 Msa0616650:five_prime_UTR 55.0%
! AGCTGAACCGGTTCAGGAAC+CGG - chr4_2:66585828-66585847 None:intergenic 55.0%
AGAAGGCTTGCGAGACCTCA+AGG - chr4_2:66586921-66586940 None:intergenic 55.0%
GGTGGCCGATGTTTGAACTC+CGG + chr4_2:66588359-66588378 Msa0616650:intron 55.0%
! TTGTGGAGAAGTGGGGTGTC+TGG + chr4_2:66588529-66588548 Msa0616650:intron 55.0%
! TGTGGAGAAGTGGGGTGTCT+GGG + chr4_2:66588530-66588549 Msa0616650:intron 55.0%
!! CGTGGAGGTGGCTTTTGAGT+AGG + chr4_2:66589989-66590008 Msa0616650:intron 55.0%
GGTGTCTAAGTAGGGAGGGA+GGG + chr4_2:66590061-66590080 Msa0616650:intron 55.0%
GGAGATAAGAGTGGTTGCGG+TGG + chr4_2:66590082-66590101 Msa0616650:five_prime_UTR 55.0%
GATAAGAGTGGTTGCGGTGG+GGG + chr4_2:66590085-66590104 Msa0616650:five_prime_UTR 55.0%
!! GGTGCTGTTCTGGTGGATTG+TGG + chr4_2:66590141-66590160 Msa0616650:CDS 55.0%
! TGTGTGTTGCTGCTGGTGCT+TGG + chr4_2:66590219-66590238 Msa0616650:CDS 55.0%
TGCTGTGCAACCAACTGCTG+AGG - chr4_2:66590255-66590274 None:intergenic 55.0%
GCAGTTGGTTGCACAGCAGT+TGG + chr4_2:66590257-66590276 Msa0616650:CDS 55.0%
!! TGTTGGTGGCGTGGGAACAG+AGG + chr4_2:66585749-66585768 Msa0616650:intron 60.0%
AACCGACCCGGTTCCTGAAC+CGG + chr4_2:66585818-66585837 Msa0616650:intron 60.0%
! AACCGGTTCAGGAACCGGGT+CGG - chr4_2:66585823-66585842 None:intergenic 60.0%
! GCTGAACCGGTTCAGGAACC+GGG - chr4_2:66585827-66585846 None:intergenic 60.0%
GGGTGCCTAGCAGCAACACA+GGG - chr4_2:66585928-66585947 None:intergenic 60.0%
!! ACGGAGTACCGTGAACGACG+CGG + chr4_2:66587175-66587194 Msa0616650:intron 60.0%
TTAGGCACCCGCGTCGTTCA+CGG - chr4_2:66587186-66587205 None:intergenic 60.0%
! GGGTCGGTTACGATTCGGGA+AGG - chr4_2:66587211-66587230 None:intergenic 60.0%
TCCGGGGGTCGGTTACGATT+CGG - chr4_2:66587216-66587235 None:intergenic 60.0%
ACCGACCCCCGGATCCAAAA+AGG + chr4_2:66587223-66587242 Msa0616650:intron 60.0%
! CCCTTTTTGGATCCGGGGGT+CGG - chr4_2:66587227-66587246 None:intergenic 60.0%
CCGACCCCCGGATCCAAAAA+GGG + chr4_2:66587224-66587243 Msa0616650:intron 60.0%
! GTGGAGAAGTGGGGTGTCTG+GGG + chr4_2:66588531-66588550 Msa0616650:intron 60.0%
CGGTGTCTAAGTAGGGAGGG+AGG + chr4_2:66590060-66590079 Msa0616650:intron 60.0%
GGGAGGGAGGGAGATAAGAG+TGG + chr4_2:66590073-66590092 Msa0616650:intron 60.0%
GTCATGTTGCTGCAGCCTGC+AGG + chr4_2:66590168-66590187 Msa0616650:CDS 60.0%
GTTGCTGCAGCCTGCAGGAA+TGG + chr4_2:66590173-66590192 Msa0616650:CDS 60.0%
! GGGGTGCCTAGCAGCAACAC+AGG - chr4_2:66585929-66585948 None:intergenic 65.0%
!! CGGAGTACCGTGAACGACGC+GGG + chr4_2:66587176-66587195 Msa0616650:intron 65.0%
CCGGGGGTCGGTTACGATTC+GGG - chr4_2:66587215-66587234 None:intergenic 65.0%
CCCGAATCGTAACCGACCCC+CGG + chr4_2:66587212-66587231 Msa0616650:intron 65.0%
!! GCGGTGGGGGACTGCTGTTT+TGG + chr4_2:66590098-66590117 Msa0616650:five_prime_UTR 65.0%
! CTGGGCGTGTGTGTTGCTGC+TGG + chr4_2:66590212-66590231 Msa0616650:CDS 65.0%
!! CCGGGTCGGTTCGACCGAAC+CGG - chr4_2:66585809-66585828 None:intergenic 70.0%
!! CCGGTTCGGTCGAACCGACC+CGG + chr4_2:66585806-66585825 Msa0616650:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 66585361 66590299 66585361 ID=Msa0616650;Name=Msa0616650
chr4_2 mRNA 66585361 66590299 66585361 ID=Msa0616650-mRNA-1;Parent=Msa0616650;Name=Msa0616650-mRNA-1;_AED=0.44;_eAED=0.44;_QI=309|0|0.5|0.5|1|0.5|2|0|57
chr4_2 exon 66585361 66585614 66585361 ID=Msa0616650-mRNA-1:exon:18968;Parent=Msa0616650-mRNA-1
chr4_2 exon 66590074 66590299 66590074 ID=Msa0616650-mRNA-1:exon:18969;Parent=Msa0616650-mRNA-1
chr4_2 five_prime_UTR 66585361 66585614 66585361 ID=Msa0616650-mRNA-1:five_prime_utr;Parent=Msa0616650-mRNA-1
chr4_2 five_prime_UTR 66590074 66590128 66590074 ID=Msa0616650-mRNA-1:five_prime_utr;Parent=Msa0616650-mRNA-1
chr4_2 CDS 66590129 66590299 66590129 ID=Msa0616650-mRNA-1:cds;Parent=Msa0616650-mRNA-1
Gene Sequence

>Msa0616650

ATGCTGATTATTGGTGCTGTTCTGGTGGATTGTGGTTCTGTCATGTTGCTGCAGCCTGCAGGAATGGTGTTTTACTGTTTCTGCTGGGCGTGTGTGTTGCTGCTGGTGCTTGGCAGTTCAGTACCTCAGCAGTTGGTTGCACAGCAGTTGGTGTGTTGGGTCTTCGATTTT
Protein sequence

>Msa0616650

MLIIGAVLVDCGSVMLLQPAGMVFYCFCWACVLLLVLGSSVPQQLVAQQLVCWVFDF