AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0616740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616740 MtrunA17_Chr8g0376391 97.122 139 4 0 2 140 49 187 7.94e-97 276
Msa0616740 MtrunA17_Chr1g0160831 67.883 137 38 5 1 132 1 136 2.01e-56 172
Msa0616740 MtrunA17_Chr3g0110941 45.926 135 68 3 1 133 4 135 5.98e-36 120
Msa0616740 MtrunA17_Chr3g0110951 46.154 130 67 2 4 133 9 135 2.75e-33 114
Msa0616740 MtrunA17_Chr5g0434801 58.065 62 26 0 17 78 41 102 1.44e-20 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616740 AT1G70780.1 59.286 140 43 5 1 133 2 134 1.18e-46 148
Msa0616740 AT1G23150.1 57.664 137 50 5 1 132 1 134 4.60e-39 129
Msa0616740 AT5G37730.1 50.588 85 41 1 16 100 16 99 5.58e-25 94.4

Find 80 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACAACAATGAATGAAAT+TGG 0.133236 4_2:+66794307 None:intergenic
CTAGAGAACTTCTTTCTTCT+TGG 0.187706 4_2:-66793170 Msa0616740:exon
AGAAAACAAAATTGTGGATT+TGG 0.224296 4_2:+66794099 None:intergenic
CAAAATTGTGGATTTGGTTT+GGG 0.228667 4_2:+66794105 None:intergenic
GTTGATCAACATCAATGTTT+TGG 0.229459 4_2:-66793929 Msa0616740:CDS
TGAATTGAATTGAATTGTTC+AGG 0.235482 4_2:+66794134 None:intergenic
GACTTGGCAGCTTAGGGTTC+GGG 0.235855 4_2:+66794454 None:intergenic
TGTTACCACAAAACACATTA+TGG 0.251371 4_2:+66793098 None:intergenic
TTCTTGGAAGACTTGCTATT+TGG 0.254854 4_2:-66793154 Msa0616740:three_prime_UTR
AGAGAGAGACTTGGCAGCTT+AGG 0.281694 4_2:+66794447 None:intergenic
ACAAAATTGTGGATTTGGTT+TGG 0.297859 4_2:+66794104 None:intergenic
CGCTGGACCGATTCGATTTG+TGG 0.324818 4_2:-66793902 Msa0616740:CDS
GCCATTCCGTTGGTTGTCTC+TGG 0.349243 4_2:+66793289 None:intergenic
GGAATGGGAGCTTGAAGAAT+TGG 0.358621 4_2:-66793231 Msa0616740:CDS
TCACAACAATGAATGAAATT+GGG 0.365390 4_2:+66794308 None:intergenic
GTAACATCCTTTCCTAAGAT+AGG 0.370173 4_2:+66793808 None:intergenic
CATTGGCGCGCCGCAGGAAT+GGG 0.374626 4_2:-66793246 Msa0616740:CDS
AAAGCGATTGCAGAGGAAAC+TGG 0.376465 4_2:+66794210 None:intergenic
GAGGGAAGACTCCCTATCTT+AGG 0.387479 4_2:-66793820 Msa0616740:CDS
AGACTTGGCAGCTTAGGGTT+CGG 0.393808 4_2:+66794453 None:intergenic
TTCTCGCATCCTTAGCGCTC+TGG 0.400064 4_2:+66793956 None:intergenic
GAGAGAGACTTGGCAGCTTA+GGG 0.410520 4_2:+66794448 None:intergenic
CATGAAATCGGAATCGCTTA+GGG 0.411941 4_2:+66794275 None:intergenic
GTAGTACCAACCTTGTTTGC+AGG 0.418312 4_2:+66794176 None:intergenic
GCATTGGCGCGCCGCAGGAA+TGG 0.422874 4_2:-66793247 Msa0616740:CDS
ATTTCATTCATTGTTGTGAA+TGG 0.429725 4_2:-66794304 Msa0616740:five_prime_UTR
GCATGAAATCGGAATCGCTT+AGG 0.431676 4_2:+66794274 None:intergenic
AAGGGAGAAGCGCATGAAAT+CGG 0.432309 4_2:+66794263 None:intergenic
CGGTCGCATTGGCGCGCCGC+AGG 0.449258 4_2:-66793252 Msa0616740:CDS
ATCCGCATTGGCCATTCCGT+TGG 0.457689 4_2:+66793279 None:intergenic
AAGGGTTGGTTTGGTTTGTG+AGG 0.476822 4_2:+66794385 None:intergenic
TGCGGATGGAGCGGTCGCAT+TGG 0.499791 4_2:-66793263 Msa0616740:CDS
AGGGTTGGTTTGGTTTGTGA+GGG 0.503828 4_2:+66794386 None:intergenic
TGAAATAAAATTTGAAGTTG+TGG 0.504169 4_2:-66792932 Msa0616740:three_prime_UTR
CTGAGTCCACTGGACACCAT+TGG 0.504639 4_2:-66793352 Msa0616740:CDS
GAATTGAATTGAATTGTTCA+GGG 0.506123 4_2:+66794135 None:intergenic
AGCTCCTCCGTGTGATCCAA+TGG 0.509536 4_2:+66793336 None:intergenic
ACTTGGCAGCTTAGGGTTCG+GGG 0.512087 4_2:+66794455 None:intergenic
AGGGGTCGACGCCGAGGCAG+CGG 0.515092 4_2:+66794154 None:intergenic
AAAATTGTGGATTTGGTTTG+GGG 0.517286 4_2:+66794106 None:intergenic
GCTTTCGCTTCCTGCAAACA+AGG 0.520829 4_2:-66794186 Msa0616740:five_prime_UTR
CACAACAATGAATGAAATTG+GGG 0.526160 4_2:+66794309 None:intergenic
TAACATCCTTTCCTAAGATA+GGG 0.527802 4_2:+66793809 None:intergenic
AAGAAAAGAAAACAAAATTG+TGG 0.528970 4_2:+66794093 None:intergenic
TCGCTTCCTGCAAACAAGGT+TGG 0.531724 4_2:-66794182 Msa0616740:five_prime_UTR
TGTCAACACGTTGGATCTGA+TGG 0.540465 4_2:-66793769 Msa0616740:intron
TGCGACCGCTCCATCCGCAT+TGG 0.541505 4_2:+66793267 None:intergenic
AACGGAATGGCCAATGCGGA+TGG 0.542673 4_2:-66793277 Msa0616740:CDS
GAGTCTTCCCTCACGAGCAT+AGG 0.565718 4_2:+66793831 None:intergenic
CGAGGGAGACCTTGTTGCCA+CGG 0.566086 4_2:-66793875 Msa0616740:CDS
GGGTTGGTTTGGTTTGTGAG+GGG 0.567652 4_2:+66794387 None:intergenic
TCGCTAACCACAAATCGAAT+CGG 0.569838 4_2:+66793895 None:intergenic
AATGAAATTGGGGATTATTG+TGG 0.575237 4_2:+66794319 None:intergenic
GGTTGGTACTACCGCTGCCT+CGG 0.576849 4_2:-66794165 Msa0616740:five_prime_UTR
GCCAGAGACAACCAACGGAA+TGG 0.577142 4_2:-66793290 Msa0616740:CDS
AATTGAATTGAATTGTTCAG+GGG 0.584878 4_2:+66794136 None:intergenic
CTGGACACCATTGGATCACA+CGG 0.585651 4_2:-66793343 Msa0616740:CDS
ATTTATAGATGTTGAAACGA+GGG 0.586457 4_2:+66792856 None:intergenic
GAAAATTTGAACAATACGCA+AGG 0.589020 4_2:+66794047 None:intergenic
AAGACTCCCTATCTTAGGAA+AGG 0.603187 4_2:-66793815 Msa0616740:CDS
CTCAAATCCTATGCTCGTGA+GGG 0.605690 4_2:-66793838 Msa0616740:CDS
TCTCAAATCCTATGCTCGTG+AGG 0.606550 4_2:-66793839 Msa0616740:CDS
GCTCTTTACTGTCAACACGT+TGG 0.610089 4_2:-66793778 Msa0616740:CDS
GAAGAAGAACCAGAGCGCTA+AGG 0.610725 4_2:-66793965 Msa0616740:CDS
ACATAAACTGAATTTAAATG+TGG 0.612068 4_2:+66793066 None:intergenic
ATTCGATTTGTGGTTAGCGA+GGG 0.615327 4_2:-66793892 Msa0616740:CDS
CTTGGCAGCTTAGGGTTCGG+GGG 0.617465 4_2:+66794456 None:intergenic
TTGTTCAGGGGTCGACGCCG+AGG 0.618773 4_2:+66794148 None:intergenic
GATTCGATTTGTGGTTAGCG+AGG 0.620699 4_2:-66793893 Msa0616740:CDS
TATCAATAACCGTGGCAACA+AGG 0.624010 4_2:+66793866 None:intergenic
AATGGCCAATGCGGATGGAG+CGG 0.628022 4_2:-66793272 Msa0616740:CDS
AAATTGTGGATTTGGTTTGG+GGG 0.633219 4_2:+66794107 None:intergenic
AATTTATAGATGTTGAAACG+AGG 0.654840 4_2:+66792855 None:intergenic
AACCAACGGAATGGCCAATG+CGG 0.659288 4_2:-66793281 Msa0616740:CDS
TGTGATCCAATGGTGTCCAG+TGG 0.669646 4_2:+66793346 None:intergenic
GAAAGCAAAAGCGATTGCAG+AGG 0.674182 4_2:+66794203 None:intergenic
TCTTCAAGCTCCCATTCCTG+CGG 0.678116 4_2:+66793236 None:intergenic
GAGAGCAGTATCAATAACCG+TGG 0.708477 4_2:+66793858 None:intergenic
AAGAAGCCAGAGACAACCAA+CGG 0.709491 4_2:-66793295 Msa0616740:CDS
GACACCATTGGATCACACGG+AGG 0.754267 4_2:-66793340 Msa0616740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTTTCTTTTTCAATT+TGG - chr4_2:66793177-66793196 Msa0616740:exon 15.0%
!! AAAATCTATTTATGTTTATG+AGG - chr4_2:66793753-66793772 Msa0616740:intron 15.0%
!! TTACATTATGATGTTAAATT+GGG - chr4_2:66793815-66793834 Msa0616740:CDS 15.0%
!!! TTTTCTTTTCTTTTTCAATT+TGG - chr4_2:66793177-66793196 Msa0616740:exon 15.0%
!! AAAATCTATTTATGTTTATG+AGG - chr4_2:66793753-66793772 Msa0616740:intron 15.0%
!! TTACATTATGATGTTAAATT+GGG - chr4_2:66793815-66793834 Msa0616740:CDS 15.0%
!! AGAGATGAAGAAAAAAATAA+GGG + chr4_2:66792895-66792914 None:intergenic 20.0%
!! AAGAAAAGAAAACAAAATTG+TGG + chr4_2:66793169-66793188 None:intergenic 20.0%
!!! CAATTTTCATTTTGTTGAAT+TGG - chr4_2:66793548-66793567 Msa0616740:intron 20.0%
!! AAAAATACGTTTGAATTCAA+TGG + chr4_2:66793708-66793727 None:intergenic 20.0%
!!! TTCAAACGTATTTTTAGTAA+TGG - chr4_2:66793713-66793732 Msa0616740:intron 20.0%
!! CTTACATTATGATGTTAAAT+TGG - chr4_2:66793814-66793833 Msa0616740:CDS 20.0%
!! TAATCAAAACTTTGCATAAA+AGG + chr4_2:66793859-66793878 None:intergenic 20.0%
!! ACATAAACTGAATTTAAATG+TGG + chr4_2:66794196-66794215 None:intergenic 20.0%
!!! TGAAATAAAATTTGAAGTTG+TGG - chr4_2:66794327-66794346 Msa0616740:five_prime_UTR 20.0%
!! AGAGATGAAGAAAAAAATAA+GGG + chr4_2:66792895-66792914 None:intergenic 20.0%
!! AAGAAAAGAAAACAAAATTG+TGG + chr4_2:66793169-66793188 None:intergenic 20.0%
!!! CAATTTTCATTTTGTTGAAT+TGG - chr4_2:66793548-66793567 Msa0616740:intron 20.0%
!! AAAAATACGTTTGAATTCAA+TGG + chr4_2:66793708-66793727 None:intergenic 20.0%
!!! TTCAAACGTATTTTTAGTAA+TGG - chr4_2:66793713-66793732 Msa0616740:intron 20.0%
!! CTTACATTATGATGTTAAAT+TGG - chr4_2:66793814-66793833 Msa0616740:CDS 20.0%
!! TAATCAAAACTTTGCATAAA+AGG + chr4_2:66793859-66793878 None:intergenic 20.0%
!! ACATAAACTGAATTTAAATG+TGG + chr4_2:66794196-66794215 None:intergenic 20.0%
!!! TGAAATAAAATTTGAAGTTG+TGG - chr4_2:66794327-66794346 Msa0616740:five_prime_UTR 20.0%
! ATGAAGAAAAAAATAAGGGT+TGG + chr4_2:66792891-66792910 None:intergenic 25.0%
! GAGAGATGAAGAAAAAAATA+AGG + chr4_2:66792896-66792915 None:intergenic 25.0%
! TCACAACAATGAATGAAATT+GGG + chr4_2:66792954-66792973 None:intergenic 25.0%
! TTCACAACAATGAATGAAAT+TGG + chr4_2:66792955-66792974 None:intergenic 25.0%
! ATTTCATTCATTGTTGTGAA+TGG - chr4_2:66792955-66792974 Msa0616740:three_prime_UTR 25.0%
! AATTGAATTGAATTGTTCAG+GGG + chr4_2:66793126-66793145 None:intergenic 25.0%
! GAATTGAATTGAATTGTTCA+GGG + chr4_2:66793127-66793146 None:intergenic 25.0%
! TGAATTGAATTGAATTGTTC+AGG + chr4_2:66793128-66793147 None:intergenic 25.0%
! AGAAAACAAAATTGTGGATT+TGG + chr4_2:66793163-66793182 None:intergenic 25.0%
!! TTGATCAACATCAATGTTTT+GGG - chr4_2:66793331-66793350 Msa0616740:CDS 25.0%
!!! TGTTGTTTTCATTGTTGAAT+TGG - chr4_2:66793575-66793594 Msa0616740:intron 25.0%
!!! GTTGTTTTCATTGTTGAATT+GGG - chr4_2:66793576-66793595 Msa0616740:intron 25.0%
! TTATGAGGAATTTCATGTTT+CGG - chr4_2:66793768-66793787 Msa0616740:intron 25.0%
! ATTATGATGTTAAATTGGGT+TGG - chr4_2:66793819-66793838 Msa0616740:CDS 25.0%
!!! GATTAGTGTCTTTTATTTTG+TGG - chr4_2:66793874-66793893 Msa0616740:CDS 25.0%
! ATTTATAGATGTTGAAACGA+GGG + chr4_2:66794406-66794425 None:intergenic 25.0%
! AATTTATAGATGTTGAAACG+AGG + chr4_2:66794407-66794426 None:intergenic 25.0%
! ATGAAGAAAAAAATAAGGGT+TGG + chr4_2:66792891-66792910 None:intergenic 25.0%
! GAGAGATGAAGAAAAAAATA+AGG + chr4_2:66792896-66792915 None:intergenic 25.0%
! TCACAACAATGAATGAAATT+GGG + chr4_2:66792954-66792973 None:intergenic 25.0%
! TTCACAACAATGAATGAAAT+TGG + chr4_2:66792955-66792974 None:intergenic 25.0%
! ATTTCATTCATTGTTGTGAA+TGG - chr4_2:66792955-66792974 Msa0616740:three_prime_UTR 25.0%
! AATTGAATTGAATTGTTCAG+GGG + chr4_2:66793126-66793145 None:intergenic 25.0%
! GAATTGAATTGAATTGTTCA+GGG + chr4_2:66793127-66793146 None:intergenic 25.0%
! TGAATTGAATTGAATTGTTC+AGG + chr4_2:66793128-66793147 None:intergenic 25.0%
! AGAAAACAAAATTGTGGATT+TGG + chr4_2:66793163-66793182 None:intergenic 25.0%
!! TTGATCAACATCAATGTTTT+GGG - chr4_2:66793331-66793350 Msa0616740:CDS 25.0%
!!! TGTTGTTTTCATTGTTGAAT+TGG - chr4_2:66793575-66793594 Msa0616740:intron 25.0%
!!! GTTGTTTTCATTGTTGAATT+GGG - chr4_2:66793576-66793595 Msa0616740:intron 25.0%
! TTATGAGGAATTTCATGTTT+CGG - chr4_2:66793768-66793787 Msa0616740:intron 25.0%
! ATTATGATGTTAAATTGGGT+TGG - chr4_2:66793819-66793838 Msa0616740:CDS 25.0%
!!! GATTAGTGTCTTTTATTTTG+TGG - chr4_2:66793874-66793893 Msa0616740:CDS 25.0%
! ATTTATAGATGTTGAAACGA+GGG + chr4_2:66794406-66794425 None:intergenic 25.0%
! AATTTATAGATGTTGAAACG+AGG + chr4_2:66794407-66794426 None:intergenic 25.0%
!! GAAAAAAATAAGGGTTGGTT+TGG + chr4_2:66792886-66792905 None:intergenic 30.0%
AATGAAATTGGGGATTATTG+TGG + chr4_2:66792943-66792962 None:intergenic 30.0%
CACAACAATGAATGAAATTG+GGG + chr4_2:66792953-66792972 None:intergenic 30.0%
!! AAAATTGTGGATTTGGTTTG+GGG + chr4_2:66793156-66793175 None:intergenic 30.0%
! CAAAATTGTGGATTTGGTTT+GGG + chr4_2:66793157-66793176 None:intergenic 30.0%
! ACAAAATTGTGGATTTGGTT+TGG + chr4_2:66793158-66793177 None:intergenic 30.0%
GAAAATTTGAACAATACGCA+AGG + chr4_2:66793215-66793234 None:intergenic 30.0%
! TGCGTATTGTTCAAATTTTC+CGG - chr4_2:66793215-66793234 Msa0616740:CDS 30.0%
! GTTGATCAACATCAATGTTT+TGG - chr4_2:66793330-66793349 Msa0616740:CDS 30.0%
TAACATCCTTTCCTAAGATA+GGG + chr4_2:66793453-66793472 None:intergenic 30.0%
! TTTTCATTGTTGAATTGGGT+AGG - chr4_2:66793580-66793599 Msa0616740:intron 30.0%
GAATTGGGTAGGAAAAAATT+TGG - chr4_2:66793591-66793610 Msa0616740:intron 30.0%
! TTTATAACCACACTTGCTTT+TGG - chr4_2:66793642-66793661 Msa0616740:intron 30.0%
!!! GCTTTTGGTTTTGTTTGTTA+TGG - chr4_2:66793657-66793676 Msa0616740:intron 30.0%
TATGAGGAATTTCATGTTTC+GGG - chr4_2:66793769-66793788 Msa0616740:intron 30.0%
! TAGTACCATAATGTGTTTTG+TGG - chr4_2:66794156-66794175 Msa0616740:five_prime_UTR 30.0%
TGTTACCACAAAACACATTA+TGG + chr4_2:66794164-66794183 None:intergenic 30.0%
!! GAAAAAAATAAGGGTTGGTT+TGG + chr4_2:66792886-66792905 None:intergenic 30.0%
AATGAAATTGGGGATTATTG+TGG + chr4_2:66792943-66792962 None:intergenic 30.0%
CACAACAATGAATGAAATTG+GGG + chr4_2:66792953-66792972 None:intergenic 30.0%
!! AAAATTGTGGATTTGGTTTG+GGG + chr4_2:66793156-66793175 None:intergenic 30.0%
! CAAAATTGTGGATTTGGTTT+GGG + chr4_2:66793157-66793176 None:intergenic 30.0%
! ACAAAATTGTGGATTTGGTT+TGG + chr4_2:66793158-66793177 None:intergenic 30.0%
GAAAATTTGAACAATACGCA+AGG + chr4_2:66793215-66793234 None:intergenic 30.0%
! TGCGTATTGTTCAAATTTTC+CGG - chr4_2:66793215-66793234 Msa0616740:CDS 30.0%
! GTTGATCAACATCAATGTTT+TGG - chr4_2:66793330-66793349 Msa0616740:CDS 30.0%
TAACATCCTTTCCTAAGATA+GGG + chr4_2:66793453-66793472 None:intergenic 30.0%
! TTTTCATTGTTGAATTGGGT+AGG - chr4_2:66793580-66793599 Msa0616740:intron 30.0%
GAATTGGGTAGGAAAAAATT+TGG - chr4_2:66793591-66793610 Msa0616740:intron 30.0%
! TTTATAACCACACTTGCTTT+TGG - chr4_2:66793642-66793661 Msa0616740:intron 30.0%
!!! GCTTTTGGTTTTGTTTGTTA+TGG - chr4_2:66793657-66793676 Msa0616740:intron 30.0%
TATGAGGAATTTCATGTTTC+GGG - chr4_2:66793769-66793788 Msa0616740:intron 30.0%
! TAGTACCATAATGTGTTTTG+TGG - chr4_2:66794156-66794175 Msa0616740:five_prime_UTR 30.0%
TGTTACCACAAAACACATTA+TGG + chr4_2:66794164-66794183 None:intergenic 30.0%
! CTTCTCCCTTTTCAAAACAA+GGG - chr4_2:66793009-66793028 Msa0616740:three_prime_UTR 35.0%
!! AAATTGTGGATTTGGTTTGG+GGG + chr4_2:66793155-66793174 None:intergenic 35.0%
AACCAAAAAAACCTTCGATC+CGG + chr4_2:66793237-66793256 None:intergenic 35.0%
GTAACATCCTTTCCTAAGAT+AGG + chr4_2:66793454-66793473 None:intergenic 35.0%
AACAAAACCAAAAGCAAGTG+TGG + chr4_2:66793652-66793671 None:intergenic 35.0%
ATGAGGAATTTCATGTTTCG+GGG - chr4_2:66793770-66793789 Msa0616740:CDS 35.0%
CTAGAGAACTTCTTTCTTCT+TGG - chr4_2:66794089-66794108 Msa0616740:five_prime_UTR 35.0%
!! TTCTTGGAAGACTTGCTATT+TGG - chr4_2:66794105-66794124 Msa0616740:five_prime_UTR 35.0%
! CTTCTCCCTTTTCAAAACAA+GGG - chr4_2:66793009-66793028 Msa0616740:three_prime_UTR 35.0%
!! AAATTGTGGATTTGGTTTGG+GGG + chr4_2:66793155-66793174 None:intergenic 35.0%
AACCAAAAAAACCTTCGATC+CGG + chr4_2:66793237-66793256 None:intergenic 35.0%
GTAACATCCTTTCCTAAGAT+AGG + chr4_2:66793454-66793473 None:intergenic 35.0%
AACAAAACCAAAAGCAAGTG+TGG + chr4_2:66793652-66793671 None:intergenic 35.0%
ATGAGGAATTTCATGTTTCG+GGG - chr4_2:66793770-66793789 Msa0616740:CDS 35.0%
CTAGAGAACTTCTTTCTTCT+TGG - chr4_2:66794089-66794108 Msa0616740:five_prime_UTR 35.0%
!! TTCTTGGAAGACTTGCTATT+TGG - chr4_2:66794105-66794124 Msa0616740:five_prime_UTR 35.0%
! TTTCTTTTTCTCTCTCGCAG+TGG - chr4_2:66792917-66792936 Msa0616740:three_prime_UTR 40.0%
CATGAAATCGGAATCGCTTA+GGG + chr4_2:66792987-66793006 None:intergenic 40.0%
! GCTTCTCCCTTTTCAAAACA+AGG - chr4_2:66793008-66793027 Msa0616740:three_prime_UTR 40.0%
! GTTCAAATTTTCCGGATCGA+AGG - chr4_2:66793223-66793242 Msa0616740:CDS 40.0%
!!! TTCCGGATCGAAGGTTTTTT+TGG - chr4_2:66793232-66793251 Msa0616740:CDS 40.0%
TCGCTAACCACAAATCGAAT+CGG + chr4_2:66793367-66793386 None:intergenic 40.0%
! ATTCGATTTGTGGTTAGCGA+GGG - chr4_2:66793367-66793386 Msa0616740:intron 40.0%
TATCAATAACCGTGGCAACA+AGG + chr4_2:66793396-66793415 None:intergenic 40.0%
AAGACTCCCTATCTTAGGAA+AGG - chr4_2:66793444-66793463 Msa0616740:intron 40.0%
! TTTCTTTTTCTCTCTCGCAG+TGG - chr4_2:66792917-66792936 Msa0616740:three_prime_UTR 40.0%
CATGAAATCGGAATCGCTTA+GGG + chr4_2:66792987-66793006 None:intergenic 40.0%
! GCTTCTCCCTTTTCAAAACA+AGG - chr4_2:66793008-66793027 Msa0616740:three_prime_UTR 40.0%
! GTTCAAATTTTCCGGATCGA+AGG - chr4_2:66793223-66793242 Msa0616740:CDS 40.0%
!!! TTCCGGATCGAAGGTTTTTT+TGG - chr4_2:66793232-66793251 Msa0616740:CDS 40.0%
TCGCTAACCACAAATCGAAT+CGG + chr4_2:66793367-66793386 None:intergenic 40.0%
! ATTCGATTTGTGGTTAGCGA+GGG - chr4_2:66793367-66793386 Msa0616740:intron 40.0%
TATCAATAACCGTGGCAACA+AGG + chr4_2:66793396-66793415 None:intergenic 40.0%
AAGACTCCCTATCTTAGGAA+AGG - chr4_2:66793444-66793463 Msa0616740:intron 40.0%
!! AGGGTTGGTTTGGTTTGTGA+GGG + chr4_2:66792876-66792895 None:intergenic 45.0%
!! AAGGGTTGGTTTGGTTTGTG+AGG + chr4_2:66792877-66792896 None:intergenic 45.0%
GCATGAAATCGGAATCGCTT+AGG + chr4_2:66792988-66793007 None:intergenic 45.0%
! AAGGGAGAAGCGCATGAAAT+CGG + chr4_2:66792999-66793018 None:intergenic 45.0%
!!! GCTCGCCCTTGTTTTGAAAA+GGG + chr4_2:66793017-66793036 None:intergenic 45.0%
AAAGCGATTGCAGAGGAAAC+TGG + chr4_2:66793052-66793071 None:intergenic 45.0%
GAAAGCAAAAGCGATTGCAG+AGG + chr4_2:66793059-66793078 None:intergenic 45.0%
! GTAGTACCAACCTTGTTTGC+AGG + chr4_2:66793086-66793105 None:intergenic 45.0%
!!! ATCAATGTTTTGGGAAGCGC+TGG - chr4_2:66793340-66793359 Msa0616740:CDS 45.0%
! GATTCGATTTGTGGTTAGCG+AGG - chr4_2:66793366-66793385 Msa0616740:intron 45.0%
GAGAGCAGTATCAATAACCG+TGG + chr4_2:66793404-66793423 None:intergenic 45.0%
TCTCAAATCCTATGCTCGTG+AGG - chr4_2:66793420-66793439 Msa0616740:intron 45.0%
CTCAAATCCTATGCTCGTGA+GGG - chr4_2:66793421-66793440 Msa0616740:intron 45.0%
GCTCTTTACTGTCAACACGT+TGG - chr4_2:66793481-66793500 Msa0616740:intron 45.0%
TGTCAACACGTTGGATCTGA+TGG - chr4_2:66793490-66793509 Msa0616740:intron 45.0%
AAGAAGCCAGAGACAACCAA+CGG - chr4_2:66793964-66793983 Msa0616740:CDS 45.0%
GGAATGGGAGCTTGAAGAAT+TGG - chr4_2:66794028-66794047 Msa0616740:five_prime_UTR 45.0%
!! AGGGTTGGTTTGGTTTGTGA+GGG + chr4_2:66792876-66792895 None:intergenic 45.0%
!! AAGGGTTGGTTTGGTTTGTG+AGG + chr4_2:66792877-66792896 None:intergenic 45.0%
GCATGAAATCGGAATCGCTT+AGG + chr4_2:66792988-66793007 None:intergenic 45.0%
! AAGGGAGAAGCGCATGAAAT+CGG + chr4_2:66792999-66793018 None:intergenic 45.0%
!!! GCTCGCCCTTGTTTTGAAAA+GGG + chr4_2:66793017-66793036 None:intergenic 45.0%
AAAGCGATTGCAGAGGAAAC+TGG + chr4_2:66793052-66793071 None:intergenic 45.0%
GAAAGCAAAAGCGATTGCAG+AGG + chr4_2:66793059-66793078 None:intergenic 45.0%
! GTAGTACCAACCTTGTTTGC+AGG + chr4_2:66793086-66793105 None:intergenic 45.0%
!!! ATCAATGTTTTGGGAAGCGC+TGG - chr4_2:66793340-66793359 Msa0616740:CDS 45.0%
! GATTCGATTTGTGGTTAGCG+AGG - chr4_2:66793366-66793385 Msa0616740:intron 45.0%
GAGAGCAGTATCAATAACCG+TGG + chr4_2:66793404-66793423 None:intergenic 45.0%
TCTCAAATCCTATGCTCGTG+AGG - chr4_2:66793420-66793439 Msa0616740:intron 45.0%
CTCAAATCCTATGCTCGTGA+GGG - chr4_2:66793421-66793440 Msa0616740:intron 45.0%
GCTCTTTACTGTCAACACGT+TGG - chr4_2:66793481-66793500 Msa0616740:intron 45.0%
TGTCAACACGTTGGATCTGA+TGG - chr4_2:66793490-66793509 Msa0616740:intron 45.0%
AAGAAGCCAGAGACAACCAA+CGG - chr4_2:66793964-66793983 Msa0616740:CDS 45.0%
GGAATGGGAGCTTGAAGAAT+TGG - chr4_2:66794028-66794047 Msa0616740:five_prime_UTR 45.0%
AGACTTGGCAGCTTAGGGTT+CGG + chr4_2:66792809-66792828 None:intergenic 50.0%
GAGAGAGACTTGGCAGCTTA+GGG + chr4_2:66792814-66792833 None:intergenic 50.0%
AGAGAGAGACTTGGCAGCTT+AGG + chr4_2:66792815-66792834 None:intergenic 50.0%
TAGCGAGAGAGAGAGAGACT+TGG + chr4_2:66792824-66792843 None:intergenic 50.0%
!! GGGTTGGTTTGGTTTGTGAG+GGG + chr4_2:66792875-66792894 None:intergenic 50.0%
!!! CGCTCGCCCTTGTTTTGAAA+AGG + chr4_2:66793018-66793037 None:intergenic 50.0%
GCTTTCGCTTCCTGCAAACA+AGG - chr4_2:66793073-66793092 Msa0616740:three_prime_UTR 50.0%
TCGCTTCCTGCAAACAAGGT+TGG - chr4_2:66793077-66793096 Msa0616740:three_prime_UTR 50.0%
! GAAGAAGAACCAGAGCGCTA+AGG - chr4_2:66793294-66793313 Msa0616740:CDS 50.0%
GAGGGAAGACTCCCTATCTT+AGG - chr4_2:66793439-66793458 Msa0616740:intron 50.0%
!!! TTTTGCAGCTCTGAGTCCAC+TGG - chr4_2:66793897-66793916 Msa0616740:CDS 50.0%
TGTGATCCAATGGTGTCCAG+TGG + chr4_2:66793916-66793935 None:intergenic 50.0%
CTGGACACCATTGGATCACA+CGG - chr4_2:66793916-66793935 Msa0616740:CDS 50.0%
AACCAACGGAATGGCCAATG+CGG - chr4_2:66793978-66793997 Msa0616740:CDS 50.0%
TCTTCAAGCTCCCATTCCTG+CGG + chr4_2:66794026-66794045 None:intergenic 50.0%
AGACTTGGCAGCTTAGGGTT+CGG + chr4_2:66792809-66792828 None:intergenic 50.0%
GAGAGAGACTTGGCAGCTTA+GGG + chr4_2:66792814-66792833 None:intergenic 50.0%
AGAGAGAGACTTGGCAGCTT+AGG + chr4_2:66792815-66792834 None:intergenic 50.0%
TAGCGAGAGAGAGAGAGACT+TGG + chr4_2:66792824-66792843 None:intergenic 50.0%
!! GGGTTGGTTTGGTTTGTGAG+GGG + chr4_2:66792875-66792894 None:intergenic 50.0%
!!! CGCTCGCCCTTGTTTTGAAA+AGG + chr4_2:66793018-66793037 None:intergenic 50.0%
GCTTTCGCTTCCTGCAAACA+AGG - chr4_2:66793073-66793092 Msa0616740:three_prime_UTR 50.0%
TCGCTTCCTGCAAACAAGGT+TGG - chr4_2:66793077-66793096 Msa0616740:three_prime_UTR 50.0%
! GAAGAAGAACCAGAGCGCTA+AGG - chr4_2:66793294-66793313 Msa0616740:CDS 50.0%
GAGGGAAGACTCCCTATCTT+AGG - chr4_2:66793439-66793458 Msa0616740:intron 50.0%
!!! TTTTGCAGCTCTGAGTCCAC+TGG - chr4_2:66793897-66793916 Msa0616740:CDS 50.0%
TGTGATCCAATGGTGTCCAG+TGG + chr4_2:66793916-66793935 None:intergenic 50.0%
CTGGACACCATTGGATCACA+CGG - chr4_2:66793916-66793935 Msa0616740:CDS 50.0%
AACCAACGGAATGGCCAATG+CGG - chr4_2:66793978-66793997 Msa0616740:CDS 50.0%
TCTTCAAGCTCCCATTCCTG+CGG + chr4_2:66794026-66794045 None:intergenic 50.0%
ACTTGGCAGCTTAGGGTTCG+GGG + chr4_2:66792807-66792826 None:intergenic 55.0%
GACTTGGCAGCTTAGGGTTC+GGG + chr4_2:66792808-66792827 None:intergenic 55.0%
TTCTCGCATCCTTAGCGCTC+TGG + chr4_2:66793306-66793325 None:intergenic 55.0%
!! CGCTGGACCGATTCGATTTG+TGG - chr4_2:66793357-66793376 Msa0616740:CDS 55.0%
GAGTCTTCCCTCACGAGCAT+AGG + chr4_2:66793431-66793450 None:intergenic 55.0%
CTGAGTCCACTGGACACCAT+TGG - chr4_2:66793907-66793926 Msa0616740:CDS 55.0%
GACACCATTGGATCACACGG+AGG - chr4_2:66793919-66793938 Msa0616740:CDS 55.0%
AGCTCCTCCGTGTGATCCAA+TGG + chr4_2:66793926-66793945 None:intergenic 55.0%
GCCAGAGACAACCAACGGAA+TGG - chr4_2:66793969-66793988 Msa0616740:CDS 55.0%
! GCCATTCCGTTGGTTGTCTC+TGG + chr4_2:66793973-66793992 None:intergenic 55.0%
! ATCCGCATTGGCCATTCCGT+TGG + chr4_2:66793983-66794002 None:intergenic 55.0%
AACGGAATGGCCAATGCGGA+TGG - chr4_2:66793982-66794001 Msa0616740:CDS 55.0%
AATGGCCAATGCGGATGGAG+CGG - chr4_2:66793987-66794006 Msa0616740:exon 55.0%
ACTTGGCAGCTTAGGGTTCG+GGG + chr4_2:66792807-66792826 None:intergenic 55.0%
GACTTGGCAGCTTAGGGTTC+GGG + chr4_2:66792808-66792827 None:intergenic 55.0%
TTCTCGCATCCTTAGCGCTC+TGG + chr4_2:66793306-66793325 None:intergenic 55.0%
!! CGCTGGACCGATTCGATTTG+TGG - chr4_2:66793357-66793376 Msa0616740:CDS 55.0%
GAGTCTTCCCTCACGAGCAT+AGG + chr4_2:66793431-66793450 None:intergenic 55.0%
CTGAGTCCACTGGACACCAT+TGG - chr4_2:66793907-66793926 Msa0616740:CDS 55.0%
GACACCATTGGATCACACGG+AGG - chr4_2:66793919-66793938 Msa0616740:CDS 55.0%
AGCTCCTCCGTGTGATCCAA+TGG + chr4_2:66793926-66793945 None:intergenic 55.0%
GCCAGAGACAACCAACGGAA+TGG - chr4_2:66793969-66793988 Msa0616740:CDS 55.0%
! GCCATTCCGTTGGTTGTCTC+TGG + chr4_2:66793973-66793992 None:intergenic 55.0%
! ATCCGCATTGGCCATTCCGT+TGG + chr4_2:66793983-66794002 None:intergenic 55.0%
AACGGAATGGCCAATGCGGA+TGG - chr4_2:66793982-66794001 Msa0616740:CDS 55.0%
AATGGCCAATGCGGATGGAG+CGG - chr4_2:66793987-66794006 Msa0616740:exon 55.0%
! GGTTGGTACTACCGCTGCCT+CGG - chr4_2:66793094-66793113 Msa0616740:three_prime_UTR 60.0%
CGAGGGAGACCTTGTTGCCA+CGG - chr4_2:66793384-66793403 Msa0616740:intron 60.0%
! GGTTGGTACTACCGCTGCCT+CGG - chr4_2:66793094-66793113 Msa0616740:three_prime_UTR 60.0%
CGAGGGAGACCTTGTTGCCA+CGG - chr4_2:66793384-66793403 Msa0616740:intron 60.0%
TTGTTCAGGGGTCGACGCCG+AGG + chr4_2:66793114-66793133 None:intergenic 65.0%
TGCGACCGCTCCATCCGCAT+TGG + chr4_2:66793995-66794014 None:intergenic 65.0%
TGCGGATGGAGCGGTCGCAT+TGG - chr4_2:66793996-66794015 Msa0616740:exon 65.0%
CATTGGCGCGCCGCAGGAAT+GGG - chr4_2:66794013-66794032 Msa0616740:five_prime_UTR 65.0%
TTGTTCAGGGGTCGACGCCG+AGG + chr4_2:66793114-66793133 None:intergenic 65.0%
TGCGACCGCTCCATCCGCAT+TGG + chr4_2:66793995-66794014 None:intergenic 65.0%
TGCGGATGGAGCGGTCGCAT+TGG - chr4_2:66793996-66794015 Msa0616740:exon 65.0%
CATTGGCGCGCCGCAGGAAT+GGG - chr4_2:66794013-66794032 Msa0616740:five_prime_UTR 65.0%
! GCATTGGCGCGCCGCAGGAA+TGG - chr4_2:66794012-66794031 Msa0616740:five_prime_UTR 70.0%
! GCATTGGCGCGCCGCAGGAA+TGG - chr4_2:66794012-66794031 Msa0616740:five_prime_UTR 70.0%
AGGGGTCGACGCCGAGGCAG+CGG + chr4_2:66793108-66793127 None:intergenic 75.0%
AGGGGTCGACGCCGAGGCAG+CGG + chr4_2:66793108-66793127 None:intergenic 75.0%
!! CGGTCGCATTGGCGCGCCGC+AGG - chr4_2:66794007-66794026 Msa0616740:five_prime_UTR 80.0%
!! CGGTCGCATTGGCGCGCCGC+AGG - chr4_2:66794007-66794026 Msa0616740:five_prime_UTR 80.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 66792804 66794477 66792804 ID=Msa0616740;Name=Msa0616740
chr4_2 mRNA 66792804 66794477 66792804 ID=Msa0616740-mRNA-1;Parent=Msa0616740;Name=Msa0616740-mRNA-1;_AED=0.01;_eAED=0.01;_QI=473|1|1|1|1|1|2|385|140
chr4_2 exon 66792804 66793376 66792804 ID=Msa0616740-mRNA-1:exon:19049;Parent=Msa0616740-mRNA-1
chr4_2 exon 66793770 66794477 66793770 ID=Msa0616740-mRNA-1:exon:19048;Parent=Msa0616740-mRNA-1
chr4_2 five_prime_UTR 66794005 66794477 66794005 ID=Msa0616740-mRNA-1:five_prime_utr;Parent=Msa0616740-mRNA-1
chr4_2 CDS 66793770 66794004 66793770 ID=Msa0616740-mRNA-1:cds;Parent=Msa0616740-mRNA-1
chr4_2 CDS 66793189 66793376 66793189 ID=Msa0616740-mRNA-1:cds;Parent=Msa0616740-mRNA-1
chr4_2 three_prime_UTR 66792804 66793188 66792804 ID=Msa0616740-mRNA-1:three_prime_utr;Parent=Msa0616740-mRNA-1
Gene Sequence

>Msa0616740

ATGTTGTTGAGTAAGCAGAAGAAGAACCAGAGCGCTAAGGATGCGAGAAAGTTGTTGATCAACATCAATGTTTTGGGAAGCGCTGGACCGATTCGATTTGTGGTTAGCGAGGGAGACCTTGTTGCCACGGTTATTGATACTGCTCTCAAATCCTATGCTCGTGAGGGAAGACTCCCTATCTTAGGAAAGGATGTTACGAGTTTTGCTCTTTACTGTCAACACGTTGGATCTGATGCTCTGAGTCCACTGGACACCATTGGATCACACGGAGGAGCTAGAAACTTCATGCTATGCAAGAAGCCAGAGACAACCAACGGAATGGCCAATGCGGATGGAGCGGTCGCATTGGCGCGCCGCAGGAATGGGAGCTTGAAGAATTGGTTCAACAAATCTCTAAGTTCAGTTTATGTTCTCATTAGCTAG
Protein sequence

>Msa0616740

MLLSKQKKNQSAKDARKLLININVLGSAGPIRFVVSEGDLVATVIDTALKSYAREGRLPILGKDVTSFALYCQHVGSDALSPLDTIGSHGGARNFMLCKKPETTNGMANADGAVALARRRNGSLKNWFNKSLSSVYVLIS*