AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0616810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616810 MtrunA17_Chr8g0376391 97.122 139 4 0 2 140 49 187 7.94e-97 276
Msa0616810 MtrunA17_Chr1g0160831 67.883 137 38 5 1 132 1 136 2.01e-56 172
Msa0616810 MtrunA17_Chr3g0110941 45.926 135 68 3 1 133 4 135 5.98e-36 120
Msa0616810 MtrunA17_Chr3g0110951 46.154 130 67 2 4 133 9 135 2.75e-33 114
Msa0616810 MtrunA17_Chr5g0434801 58.065 62 26 0 17 78 41 102 1.44e-20 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0616810 AT1G70780.1 59.286 140 43 5 1 133 2 134 1.18e-46 148
Msa0616810 AT1G23150.1 57.664 137 50 5 1 132 1 134 4.60e-39 129
Msa0616810 AT5G37730.1 50.588 85 41 1 16 100 16 99 5.58e-25 94.4

Find 79 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACAACAATGAATGAAAT+TGG 0.133236 4_2:-67003926 None:intergenic
CTAGAGAACTTCTTTCTTCT+TGG 0.187706 4_2:+67005184 Msa0616810:exon
AGAAAACAAAATTGTGGATT+TGG 0.224296 4_2:-67004134 None:intergenic
CAAAATTGTGGATTTGGTTT+GGG 0.228667 4_2:-67004128 None:intergenic
GTTGATCAACATCAATGTTT+TGG 0.229459 4_2:+67004304 Msa0616810:CDS
TGAATTGAATTGAATTGTTC+AGG 0.235482 4_2:-67004099 None:intergenic
GACTTGGCAGCTTAGGGTTC+GGG 0.235855 4_2:-67003779 None:intergenic
TGTTACCACAAAACACATTA+TGG 0.251371 4_2:-67005256 None:intergenic
TTCTTGGAAGACTTGCTATT+TGG 0.254854 4_2:+67005200 Msa0616810:three_prime_UTR
AGAGAGAGACTTGGCAGCTT+AGG 0.281694 4_2:-67003786 None:intergenic
ACAAAATTGTGGATTTGGTT+TGG 0.297859 4_2:-67004129 None:intergenic
CGCTGGACCGATTCGATTTG+TGG 0.324818 4_2:+67004331 Msa0616810:CDS
GCCATTCCGTTGGTTGTCTC+TGG 0.349243 4_2:-67005065 None:intergenic
GGAATGGGAGCTTGAAGAAT+TGG 0.358621 4_2:+67005123 Msa0616810:CDS
TCACAACAATGAATGAAATT+GGG 0.365390 4_2:-67003925 None:intergenic
AAAGCGATTGCAGAGGAAAC+TGG 0.376465 4_2:-67004023 None:intergenic
CATTGGCTCGCCGCAGGAAT+GGG 0.380492 4_2:+67005108 Msa0616810:CDS
AGACTTGGCAGCTTAGGGTT+CGG 0.393808 4_2:-67003780 None:intergenic
GAGGGAAGACTTCCTATCTT+AGG 0.398437 4_2:+67004413 Msa0616810:CDS
TTCTCGCATCCTTAGCGCTC+TGG 0.400064 4_2:-67004277 None:intergenic
GAGAGAGACTTGGCAGCTTA+GGG 0.410520 4_2:-67003785 None:intergenic
CATGAAATCGGAATCGCTTA+GGG 0.411941 4_2:-67003958 None:intergenic
GTAGTACCAACCTTGTTTGC+AGG 0.418312 4_2:-67004057 None:intergenic
GCATTGGCTCGCCGCAGGAA+TGG 0.419805 4_2:+67005107 Msa0616810:CDS
ATTTCATTCATTGTTGTGAA+TGG 0.429725 4_2:+67003929 Msa0616810:five_prime_UTR
GCATGAAATCGGAATCGCTT+AGG 0.431676 4_2:-67003959 None:intergenic
AAGGGAGAAGCGCATGAAAT+CGG 0.432309 4_2:-67003970 None:intergenic
ATCCGCATTGGCCATTCCGT+TGG 0.457689 4_2:-67005075 None:intergenic
GTAACATCCTTTCCTAAGAT+AGG 0.464705 4_2:-67004425 None:intergenic
AAGGGTTGGTTTGGTTTGTG+AGG 0.476822 4_2:-67003848 None:intergenic
CGGTCGCATTGGCTCGCCGC+AGG 0.490804 4_2:+67005102 Msa0616810:CDS
AGGGTTGGTTTGGTTTGTGA+GGG 0.503828 4_2:-67003847 None:intergenic
TGAAATAAAATTTGAAGTTG+TGG 0.504169 4_2:+67005422 Msa0616810:three_prime_UTR
CTGAGTCCACTGGACACCAT+TGG 0.504639 4_2:+67005002 Msa0616810:CDS
GAATTGAATTGAATTGTTCA+GGG 0.506123 4_2:-67004098 None:intergenic
AGCTCCTCCGTGTGATCCAA+TGG 0.509536 4_2:-67005018 None:intergenic
TGCGGATGGAGCGGTCGCAT+TGG 0.509786 4_2:+67005091 Msa0616810:CDS
ACTTGGCAGCTTAGGGTTCG+GGG 0.512087 4_2:-67003778 None:intergenic
AGGGGTCGACGCCGAGGCAG+CGG 0.515092 4_2:-67004079 None:intergenic
AAAATTGTGGATTTGGTTTG+GGG 0.517286 4_2:-67004127 None:intergenic
GCTTTCGCTTCCTGCAAACA+AGG 0.520829 4_2:+67004047 Msa0616810:five_prime_UTR
CACAACAATGAATGAAATTG+GGG 0.526160 4_2:-67003924 None:intergenic
AAGAAAAGAAAACAAAATTG+TGG 0.528970 4_2:-67004140 None:intergenic
TCGCTTCCTGCAAACAAGGT+TGG 0.531724 4_2:+67004051 Msa0616810:five_prime_UTR
TGTCAACACGTTGGATCTGA+TGG 0.540465 4_2:+67004464 Msa0616810:CDS
TGCGACCGCTCCATCCGCAT+TGG 0.541505 4_2:-67005087 None:intergenic
AACGGAATGGCCAATGCGGA+TGG 0.542673 4_2:+67005077 Msa0616810:CDS
AAGTCTTCCCTCACGAGCAT+AGG 0.565718 4_2:-67004402 None:intergenic
CGAGGGAGACCTTGTTGCCA+CGG 0.566086 4_2:+67004358 Msa0616810:CDS
GGGTTGGTTTGGTTTGTGAG+GGG 0.567652 4_2:-67003846 None:intergenic
TCGCTAACCACAAATCGAAT+CGG 0.569838 4_2:-67004338 None:intergenic
AATGAAATTGGGGATTATTG+TGG 0.575237 4_2:-67003914 None:intergenic
GGTTGGTACTACCGCTGCCT+CGG 0.576849 4_2:+67004068 Msa0616810:five_prime_UTR
GCCAGAGACAACCAACGGAA+TGG 0.577142 4_2:+67005064 Msa0616810:CDS
AATTGAATTGAATTGTTCAG+GGG 0.584878 4_2:-67004097 None:intergenic
CTGGACACCATTGGATCACA+CGG 0.585651 4_2:+67005011 Msa0616810:CDS
ATTTATAGATGTTGAAACGA+GGG 0.586457 4_2:-67005498 None:intergenic
GAAAATTTGAACAATACGCA+AGG 0.589020 4_2:-67004186 None:intergenic
AAGACTTCCTATCTTAGGAA+AGG 0.603187 4_2:+67004418 Msa0616810:CDS
CTCAAATCCTATGCTCGTGA+GGG 0.605690 4_2:+67004395 Msa0616810:CDS
TCTCAAATCCTATGCTCGTG+AGG 0.606550 4_2:+67004394 Msa0616810:CDS
GCTCTTTACTGTCAACACGT+TGG 0.610089 4_2:+67004455 Msa0616810:CDS
GAAGAAGAACCAGAGCGCTA+AGG 0.610725 4_2:+67004268 Msa0616810:CDS
ACATAAACTGAATTTAAATG+TGG 0.612068 4_2:-67005288 None:intergenic
ATTCGATTTGTGGTTAGCGA+GGG 0.615327 4_2:+67004341 Msa0616810:CDS
CTTGGCAGCTTAGGGTTCGG+GGG 0.617465 4_2:-67003777 None:intergenic
TTGTTCAGGGGTCGACGCCG+AGG 0.618773 4_2:-67004085 None:intergenic
GATTCGATTTGTGGTTAGCG+AGG 0.620699 4_2:+67004340 Msa0616810:CDS
TATCAATAACCGTGGCAACA+AGG 0.624010 4_2:-67004367 None:intergenic
AATGGCCAATGCGGATGGAG+CGG 0.628022 4_2:+67005082 Msa0616810:CDS
AAATTGTGGATTTGGTTTGG+GGG 0.633219 4_2:-67004126 None:intergenic
AATTTATAGATGTTGAAACG+AGG 0.654840 4_2:-67005499 None:intergenic
AACCAACGGAATGGCCAATG+CGG 0.659288 4_2:+67005073 Msa0616810:CDS
TGTGATCCAATGGTGTCCAG+TGG 0.669646 4_2:-67005008 None:intergenic
GAAAGCAAAAGCGATTGCAG+AGG 0.674182 4_2:-67004030 None:intergenic
TCTTCAAGCTCCCATTCCTG+CGG 0.695043 4_2:-67005118 None:intergenic
GAGAGCAGTATCAATAACCG+TGG 0.708477 4_2:-67004375 None:intergenic
AAGAAGCCAGAGACAACCAA+CGG 0.709491 4_2:+67005059 Msa0616810:CDS
GACACCATTGGATCACACGG+AGG 0.754267 4_2:+67005014 Msa0616810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTTTCTTTTTCAATT+TGG + chr4_2:67004151-67004170 Msa0616810:five_prime_UTR 15.0%
!! AAAATCTATTTATGTTTATG+AGG + chr4_2:67004848-67004867 Msa0616810:intron 15.0%
!! TTACATTATGATGTTAAATT+GGG + chr4_2:67004910-67004929 Msa0616810:intron 15.0%
!!! TTTTCTTTTCTTTTTCAATT+TGG + chr4_2:67004151-67004170 Msa0616810:five_prime_UTR 15.0%
!! AAAATCTATTTATGTTTATG+AGG + chr4_2:67004848-67004867 Msa0616810:intron 15.0%
!! TTACATTATGATGTTAAATT+GGG + chr4_2:67004910-67004929 Msa0616810:intron 15.0%
!! AGAGATGAAGAAAAAAATAA+GGG - chr4_2:67003869-67003888 None:intergenic 20.0%
!! AAGAAAAGAAAACAAAATTG+TGG - chr4_2:67004143-67004162 None:intergenic 20.0%
!!! CAATTTTCATTTTGTTGAAT+TGG + chr4_2:67004522-67004541 Msa0616810:intron 20.0%
!! ATAATATTGTAACCTCAATT+AGG + chr4_2:67004601-67004620 Msa0616810:intron 20.0%
!! AAAAATACGTTTGAATTCAA+TGG - chr4_2:67004803-67004822 None:intergenic 20.0%
!!! TTCAAACGTATTTTTAGTAA+TGG + chr4_2:67004808-67004827 Msa0616810:intron 20.0%
!! TTATGAGGAATTTCATATTT+CGG + chr4_2:67004863-67004882 Msa0616810:intron 20.0%
!! CTTACATTATGATGTTAAAT+TGG + chr4_2:67004909-67004928 Msa0616810:intron 20.0%
!! TAATCAAAACTTTGCATAAA+AGG - chr4_2:67004954-67004973 None:intergenic 20.0%
!! ACATAAACTGAATTTAAATG+TGG - chr4_2:67005291-67005310 None:intergenic 20.0%
!!! TGAAATAAAATTTGAAGTTG+TGG + chr4_2:67005422-67005441 Msa0616810:three_prime_UTR 20.0%
!! AGAGATGAAGAAAAAAATAA+GGG - chr4_2:67003869-67003888 None:intergenic 20.0%
!! AAGAAAAGAAAACAAAATTG+TGG - chr4_2:67004143-67004162 None:intergenic 20.0%
!!! CAATTTTCATTTTGTTGAAT+TGG + chr4_2:67004522-67004541 Msa0616810:intron 20.0%
!! ATAATATTGTAACCTCAATT+AGG + chr4_2:67004601-67004620 Msa0616810:intron 20.0%
!! AAAAATACGTTTGAATTCAA+TGG - chr4_2:67004803-67004822 None:intergenic 20.0%
!!! TTCAAACGTATTTTTAGTAA+TGG + chr4_2:67004808-67004827 Msa0616810:intron 20.0%
!! TTATGAGGAATTTCATATTT+CGG + chr4_2:67004863-67004882 Msa0616810:intron 20.0%
!! CTTACATTATGATGTTAAAT+TGG + chr4_2:67004909-67004928 Msa0616810:intron 20.0%
!! TAATCAAAACTTTGCATAAA+AGG - chr4_2:67004954-67004973 None:intergenic 20.0%
!! ACATAAACTGAATTTAAATG+TGG - chr4_2:67005291-67005310 None:intergenic 20.0%
!!! TGAAATAAAATTTGAAGTTG+TGG + chr4_2:67005422-67005441 Msa0616810:three_prime_UTR 20.0%
! ATGAAGAAAAAAATAAGGGT+TGG - chr4_2:67003865-67003884 None:intergenic 25.0%
! GAGAGATGAAGAAAAAAATA+AGG - chr4_2:67003870-67003889 None:intergenic 25.0%
! TCACAACAATGAATGAAATT+GGG - chr4_2:67003928-67003947 None:intergenic 25.0%
! TTCACAACAATGAATGAAAT+TGG - chr4_2:67003929-67003948 None:intergenic 25.0%
! ATTTCATTCATTGTTGTGAA+TGG + chr4_2:67003929-67003948 Msa0616810:five_prime_UTR 25.0%
! AATTGAATTGAATTGTTCAG+GGG - chr4_2:67004100-67004119 None:intergenic 25.0%
! GAATTGAATTGAATTGTTCA+GGG - chr4_2:67004101-67004120 None:intergenic 25.0%
! TGAATTGAATTGAATTGTTC+AGG - chr4_2:67004102-67004121 None:intergenic 25.0%
! AGAAAACAAAATTGTGGATT+TGG - chr4_2:67004137-67004156 None:intergenic 25.0%
!! TTGATCAACATCAATGTTTT+GGG + chr4_2:67004305-67004324 Msa0616810:CDS 25.0%
!!! GTTGTTTTCATTGTTGAATT+TGG + chr4_2:67004550-67004569 Msa0616810:intron 25.0%
! TTCAATAACGAAAACATCAT+GGG - chr4_2:67004669-67004688 None:intergenic 25.0%
! ATTCAATAACGAAAACATCA+TGG - chr4_2:67004670-67004689 None:intergenic 25.0%
!! TGATGTTTTCGTTATTGAAT+TGG + chr4_2:67004670-67004689 Msa0616810:intron 25.0%
!! GATGTTTTCGTTATTGAATT+GGG + chr4_2:67004671-67004690 Msa0616810:intron 25.0%
! TATGAGGAATTTCATATTTC+GGG + chr4_2:67004864-67004883 Msa0616810:intron 25.0%
! ATTATGATGTTAAATTGGGT+TGG + chr4_2:67004914-67004933 Msa0616810:intron 25.0%
!!! GATTAGTGTCTTTTATTTTG+TGG + chr4_2:67004969-67004988 Msa0616810:intron 25.0%
! ATTTATAGATGTTGAAACGA+GGG - chr4_2:67005501-67005520 None:intergenic 25.0%
! AATTTATAGATGTTGAAACG+AGG - chr4_2:67005502-67005521 None:intergenic 25.0%
! ATGAAGAAAAAAATAAGGGT+TGG - chr4_2:67003865-67003884 None:intergenic 25.0%
! GAGAGATGAAGAAAAAAATA+AGG - chr4_2:67003870-67003889 None:intergenic 25.0%
! TCACAACAATGAATGAAATT+GGG - chr4_2:67003928-67003947 None:intergenic 25.0%
! TTCACAACAATGAATGAAAT+TGG - chr4_2:67003929-67003948 None:intergenic 25.0%
! ATTTCATTCATTGTTGTGAA+TGG + chr4_2:67003929-67003948 Msa0616810:five_prime_UTR 25.0%
! AATTGAATTGAATTGTTCAG+GGG - chr4_2:67004100-67004119 None:intergenic 25.0%
! GAATTGAATTGAATTGTTCA+GGG - chr4_2:67004101-67004120 None:intergenic 25.0%
! TGAATTGAATTGAATTGTTC+AGG - chr4_2:67004102-67004121 None:intergenic 25.0%
! AGAAAACAAAATTGTGGATT+TGG - chr4_2:67004137-67004156 None:intergenic 25.0%
!! TTGATCAACATCAATGTTTT+GGG + chr4_2:67004305-67004324 Msa0616810:CDS 25.0%
!!! GTTGTTTTCATTGTTGAATT+TGG + chr4_2:67004550-67004569 Msa0616810:intron 25.0%
! TTCAATAACGAAAACATCAT+GGG - chr4_2:67004669-67004688 None:intergenic 25.0%
! ATTCAATAACGAAAACATCA+TGG - chr4_2:67004670-67004689 None:intergenic 25.0%
!! TGATGTTTTCGTTATTGAAT+TGG + chr4_2:67004670-67004689 Msa0616810:intron 25.0%
!! GATGTTTTCGTTATTGAATT+GGG + chr4_2:67004671-67004690 Msa0616810:intron 25.0%
! TATGAGGAATTTCATATTTC+GGG + chr4_2:67004864-67004883 Msa0616810:intron 25.0%
! ATTATGATGTTAAATTGGGT+TGG + chr4_2:67004914-67004933 Msa0616810:intron 25.0%
!!! GATTAGTGTCTTTTATTTTG+TGG + chr4_2:67004969-67004988 Msa0616810:intron 25.0%
! ATTTATAGATGTTGAAACGA+GGG - chr4_2:67005501-67005520 None:intergenic 25.0%
! AATTTATAGATGTTGAAACG+AGG - chr4_2:67005502-67005521 None:intergenic 25.0%
!! GAAAAAAATAAGGGTTGGTT+TGG - chr4_2:67003860-67003879 None:intergenic 30.0%
AATGAAATTGGGGATTATTG+TGG - chr4_2:67003917-67003936 None:intergenic 30.0%
CACAACAATGAATGAAATTG+GGG - chr4_2:67003927-67003946 None:intergenic 30.0%
!! AAAATTGTGGATTTGGTTTG+GGG - chr4_2:67004130-67004149 None:intergenic 30.0%
! CAAAATTGTGGATTTGGTTT+GGG - chr4_2:67004131-67004150 None:intergenic 30.0%
! ACAAAATTGTGGATTTGGTT+TGG - chr4_2:67004132-67004151 None:intergenic 30.0%
GAAAATTTGAACAATACGCA+AGG - chr4_2:67004189-67004208 None:intergenic 30.0%
! TGCGTATTGTTCAAATTTTC+CGG + chr4_2:67004189-67004208 Msa0616810:five_prime_UTR 30.0%
! GTTGATCAACATCAATGTTT+TGG + chr4_2:67004304-67004323 Msa0616810:CDS 30.0%
ATTGTAACCTCAATTAGGTT+TGG + chr4_2:67004606-67004625 Msa0616810:intron 30.0%
ACAAAAACCAAACCTAATTG+AGG - chr4_2:67004616-67004635 None:intergenic 30.0%
! TTTTCGTTATTGAATTGGGT+AGG + chr4_2:67004675-67004694 Msa0616810:intron 30.0%
GAATTGGGTAGGAAAAAATT+TGG + chr4_2:67004686-67004705 Msa0616810:intron 30.0%
! TTTATAACCACACTTGCTTT+TGG + chr4_2:67004737-67004756 Msa0616810:intron 30.0%
!!! GCTTTTGGTTTTGTTTGTTA+TGG + chr4_2:67004752-67004771 Msa0616810:intron 30.0%
ATGAGGAATTTCATATTTCG+GGG + chr4_2:67004865-67004884 Msa0616810:intron 30.0%
! TAGTACCATAATGTGTTTTG+TGG + chr4_2:67005251-67005270 Msa0616810:three_prime_UTR 30.0%
TGTTACCACAAAACACATTA+TGG - chr4_2:67005259-67005278 None:intergenic 30.0%
!! GAAAAAAATAAGGGTTGGTT+TGG - chr4_2:67003860-67003879 None:intergenic 30.0%
AATGAAATTGGGGATTATTG+TGG - chr4_2:67003917-67003936 None:intergenic 30.0%
CACAACAATGAATGAAATTG+GGG - chr4_2:67003927-67003946 None:intergenic 30.0%
!! AAAATTGTGGATTTGGTTTG+GGG - chr4_2:67004130-67004149 None:intergenic 30.0%
! CAAAATTGTGGATTTGGTTT+GGG - chr4_2:67004131-67004150 None:intergenic 30.0%
! ACAAAATTGTGGATTTGGTT+TGG - chr4_2:67004132-67004151 None:intergenic 30.0%
GAAAATTTGAACAATACGCA+AGG - chr4_2:67004189-67004208 None:intergenic 30.0%
! TGCGTATTGTTCAAATTTTC+CGG + chr4_2:67004189-67004208 Msa0616810:five_prime_UTR 30.0%
! GTTGATCAACATCAATGTTT+TGG + chr4_2:67004304-67004323 Msa0616810:CDS 30.0%
ATTGTAACCTCAATTAGGTT+TGG + chr4_2:67004606-67004625 Msa0616810:intron 30.0%
ACAAAAACCAAACCTAATTG+AGG - chr4_2:67004616-67004635 None:intergenic 30.0%
! TTTTCGTTATTGAATTGGGT+AGG + chr4_2:67004675-67004694 Msa0616810:intron 30.0%
GAATTGGGTAGGAAAAAATT+TGG + chr4_2:67004686-67004705 Msa0616810:intron 30.0%
! TTTATAACCACACTTGCTTT+TGG + chr4_2:67004737-67004756 Msa0616810:intron 30.0%
!!! GCTTTTGGTTTTGTTTGTTA+TGG + chr4_2:67004752-67004771 Msa0616810:intron 30.0%
ATGAGGAATTTCATATTTCG+GGG + chr4_2:67004865-67004884 Msa0616810:intron 30.0%
! TAGTACCATAATGTGTTTTG+TGG + chr4_2:67005251-67005270 Msa0616810:three_prime_UTR 30.0%
TGTTACCACAAAACACATTA+TGG - chr4_2:67005259-67005278 None:intergenic 30.0%
! CTTCTCCCTTTTCAAAACAA+GGG + chr4_2:67003983-67004002 Msa0616810:five_prime_UTR 35.0%
!! AAATTGTGGATTTGGTTTGG+GGG - chr4_2:67004129-67004148 None:intergenic 35.0%
AACCAAAAAAACCTTCGATC+CGG - chr4_2:67004211-67004230 None:intergenic 35.0%
AAGACTTCCTATCTTAGGAA+AGG + chr4_2:67004418-67004437 Msa0616810:CDS 35.0%
GTAACATCCTTTCCTAAGAT+AGG - chr4_2:67004428-67004447 None:intergenic 35.0%
AACAAAACCAAAAGCAAGTG+TGG - chr4_2:67004747-67004766 None:intergenic 35.0%
CTAGAGAACTTCTTTCTTCT+TGG + chr4_2:67005184-67005203 Msa0616810:exon 35.0%
!! TTCTTGGAAGACTTGCTATT+TGG + chr4_2:67005200-67005219 Msa0616810:three_prime_UTR 35.0%
! CTTCTCCCTTTTCAAAACAA+GGG + chr4_2:67003983-67004002 Msa0616810:five_prime_UTR 35.0%
!! AAATTGTGGATTTGGTTTGG+GGG - chr4_2:67004129-67004148 None:intergenic 35.0%
AACCAAAAAAACCTTCGATC+CGG - chr4_2:67004211-67004230 None:intergenic 35.0%
AAGACTTCCTATCTTAGGAA+AGG + chr4_2:67004418-67004437 Msa0616810:CDS 35.0%
GTAACATCCTTTCCTAAGAT+AGG - chr4_2:67004428-67004447 None:intergenic 35.0%
AACAAAACCAAAAGCAAGTG+TGG - chr4_2:67004747-67004766 None:intergenic 35.0%
CTAGAGAACTTCTTTCTTCT+TGG + chr4_2:67005184-67005203 Msa0616810:exon 35.0%
!! TTCTTGGAAGACTTGCTATT+TGG + chr4_2:67005200-67005219 Msa0616810:three_prime_UTR 35.0%
! TTTCTTTTTCTCTCTCGCAG+TGG + chr4_2:67003891-67003910 Msa0616810:five_prime_UTR 40.0%
CATGAAATCGGAATCGCTTA+GGG - chr4_2:67003961-67003980 None:intergenic 40.0%
! GCTTCTCCCTTTTCAAAACA+AGG + chr4_2:67003982-67004001 Msa0616810:five_prime_UTR 40.0%
! GTTCAAATTTTCCGGATCGA+AGG + chr4_2:67004197-67004216 Msa0616810:five_prime_UTR 40.0%
!!! TTCCGGATCGAAGGTTTTTT+TGG + chr4_2:67004206-67004225 Msa0616810:five_prime_UTR 40.0%
TCGCTAACCACAAATCGAAT+CGG - chr4_2:67004341-67004360 None:intergenic 40.0%
! ATTCGATTTGTGGTTAGCGA+GGG + chr4_2:67004341-67004360 Msa0616810:CDS 40.0%
TATCAATAACCGTGGCAACA+AGG - chr4_2:67004370-67004389 None:intergenic 40.0%
! TTTCTTTTTCTCTCTCGCAG+TGG + chr4_2:67003891-67003910 Msa0616810:five_prime_UTR 40.0%
CATGAAATCGGAATCGCTTA+GGG - chr4_2:67003961-67003980 None:intergenic 40.0%
! GCTTCTCCCTTTTCAAAACA+AGG + chr4_2:67003982-67004001 Msa0616810:five_prime_UTR 40.0%
! GTTCAAATTTTCCGGATCGA+AGG + chr4_2:67004197-67004216 Msa0616810:five_prime_UTR 40.0%
!!! TTCCGGATCGAAGGTTTTTT+TGG + chr4_2:67004206-67004225 Msa0616810:five_prime_UTR 40.0%
TCGCTAACCACAAATCGAAT+CGG - chr4_2:67004341-67004360 None:intergenic 40.0%
! ATTCGATTTGTGGTTAGCGA+GGG + chr4_2:67004341-67004360 Msa0616810:CDS 40.0%
TATCAATAACCGTGGCAACA+AGG - chr4_2:67004370-67004389 None:intergenic 40.0%
!! AGGGTTGGTTTGGTTTGTGA+GGG - chr4_2:67003850-67003869 None:intergenic 45.0%
!! AAGGGTTGGTTTGGTTTGTG+AGG - chr4_2:67003851-67003870 None:intergenic 45.0%
GCATGAAATCGGAATCGCTT+AGG - chr4_2:67003962-67003981 None:intergenic 45.0%
! AAGGGAGAAGCGCATGAAAT+CGG - chr4_2:67003973-67003992 None:intergenic 45.0%
!!! GCTCGCCCTTGTTTTGAAAA+GGG - chr4_2:67003991-67004010 None:intergenic 45.0%
AAAGCGATTGCAGAGGAAAC+TGG - chr4_2:67004026-67004045 None:intergenic 45.0%
GAAAGCAAAAGCGATTGCAG+AGG - chr4_2:67004033-67004052 None:intergenic 45.0%
! GTAGTACCAACCTTGTTTGC+AGG - chr4_2:67004060-67004079 None:intergenic 45.0%
!!! ATCAATGTTTTGGGAAGCGC+TGG + chr4_2:67004314-67004333 Msa0616810:CDS 45.0%
! GATTCGATTTGTGGTTAGCG+AGG + chr4_2:67004340-67004359 Msa0616810:CDS 45.0%
GAGAGCAGTATCAATAACCG+TGG - chr4_2:67004378-67004397 None:intergenic 45.0%
TCTCAAATCCTATGCTCGTG+AGG + chr4_2:67004394-67004413 Msa0616810:CDS 45.0%
CTCAAATCCTATGCTCGTGA+GGG + chr4_2:67004395-67004414 Msa0616810:CDS 45.0%
GAGGGAAGACTTCCTATCTT+AGG + chr4_2:67004413-67004432 Msa0616810:CDS 45.0%
GCTCTTTACTGTCAACACGT+TGG + chr4_2:67004455-67004474 Msa0616810:CDS 45.0%
TGTCAACACGTTGGATCTGA+TGG + chr4_2:67004464-67004483 Msa0616810:CDS 45.0%
AAGAAGCCAGAGACAACCAA+CGG + chr4_2:67005059-67005078 Msa0616810:CDS 45.0%
GGAATGGGAGCTTGAAGAAT+TGG + chr4_2:67005123-67005142 Msa0616810:CDS 45.0%
!! AGGGTTGGTTTGGTTTGTGA+GGG - chr4_2:67003850-67003869 None:intergenic 45.0%
!! AAGGGTTGGTTTGGTTTGTG+AGG - chr4_2:67003851-67003870 None:intergenic 45.0%
GCATGAAATCGGAATCGCTT+AGG - chr4_2:67003962-67003981 None:intergenic 45.0%
! AAGGGAGAAGCGCATGAAAT+CGG - chr4_2:67003973-67003992 None:intergenic 45.0%
!!! GCTCGCCCTTGTTTTGAAAA+GGG - chr4_2:67003991-67004010 None:intergenic 45.0%
AAAGCGATTGCAGAGGAAAC+TGG - chr4_2:67004026-67004045 None:intergenic 45.0%
GAAAGCAAAAGCGATTGCAG+AGG - chr4_2:67004033-67004052 None:intergenic 45.0%
! GTAGTACCAACCTTGTTTGC+AGG - chr4_2:67004060-67004079 None:intergenic 45.0%
!!! ATCAATGTTTTGGGAAGCGC+TGG + chr4_2:67004314-67004333 Msa0616810:CDS 45.0%
! GATTCGATTTGTGGTTAGCG+AGG + chr4_2:67004340-67004359 Msa0616810:CDS 45.0%
GAGAGCAGTATCAATAACCG+TGG - chr4_2:67004378-67004397 None:intergenic 45.0%
TCTCAAATCCTATGCTCGTG+AGG + chr4_2:67004394-67004413 Msa0616810:CDS 45.0%
CTCAAATCCTATGCTCGTGA+GGG + chr4_2:67004395-67004414 Msa0616810:CDS 45.0%
GAGGGAAGACTTCCTATCTT+AGG + chr4_2:67004413-67004432 Msa0616810:CDS 45.0%
GCTCTTTACTGTCAACACGT+TGG + chr4_2:67004455-67004474 Msa0616810:CDS 45.0%
TGTCAACACGTTGGATCTGA+TGG + chr4_2:67004464-67004483 Msa0616810:CDS 45.0%
AAGAAGCCAGAGACAACCAA+CGG + chr4_2:67005059-67005078 Msa0616810:CDS 45.0%
GGAATGGGAGCTTGAAGAAT+TGG + chr4_2:67005123-67005142 Msa0616810:CDS 45.0%
AGACTTGGCAGCTTAGGGTT+CGG - chr4_2:67003783-67003802 None:intergenic 50.0%
GAGAGAGACTTGGCAGCTTA+GGG - chr4_2:67003788-67003807 None:intergenic 50.0%
AGAGAGAGACTTGGCAGCTT+AGG - chr4_2:67003789-67003808 None:intergenic 50.0%
TAGCGAGAGAGAGAGAGACT+TGG - chr4_2:67003798-67003817 None:intergenic 50.0%
!! GGGTTGGTTTGGTTTGTGAG+GGG - chr4_2:67003849-67003868 None:intergenic 50.0%
!!! CGCTCGCCCTTGTTTTGAAA+AGG - chr4_2:67003992-67004011 None:intergenic 50.0%
GCTTTCGCTTCCTGCAAACA+AGG + chr4_2:67004047-67004066 Msa0616810:five_prime_UTR 50.0%
TCGCTTCCTGCAAACAAGGT+TGG + chr4_2:67004051-67004070 Msa0616810:five_prime_UTR 50.0%
! GAAGAAGAACCAGAGCGCTA+AGG + chr4_2:67004268-67004287 Msa0616810:CDS 50.0%
AAGTCTTCCCTCACGAGCAT+AGG - chr4_2:67004405-67004424 None:intergenic 50.0%
!!! TTTTGCAGCTCTGAGTCCAC+TGG + chr4_2:67004992-67005011 Msa0616810:intron 50.0%
TGTGATCCAATGGTGTCCAG+TGG - chr4_2:67005011-67005030 None:intergenic 50.0%
CTGGACACCATTGGATCACA+CGG + chr4_2:67005011-67005030 Msa0616810:CDS 50.0%
AACCAACGGAATGGCCAATG+CGG + chr4_2:67005073-67005092 Msa0616810:CDS 50.0%
TCTTCAAGCTCCCATTCCTG+CGG - chr4_2:67005121-67005140 None:intergenic 50.0%
AGACTTGGCAGCTTAGGGTT+CGG - chr4_2:67003783-67003802 None:intergenic 50.0%
GAGAGAGACTTGGCAGCTTA+GGG - chr4_2:67003788-67003807 None:intergenic 50.0%
AGAGAGAGACTTGGCAGCTT+AGG - chr4_2:67003789-67003808 None:intergenic 50.0%
TAGCGAGAGAGAGAGAGACT+TGG - chr4_2:67003798-67003817 None:intergenic 50.0%
!! GGGTTGGTTTGGTTTGTGAG+GGG - chr4_2:67003849-67003868 None:intergenic 50.0%
!!! CGCTCGCCCTTGTTTTGAAA+AGG - chr4_2:67003992-67004011 None:intergenic 50.0%
GCTTTCGCTTCCTGCAAACA+AGG + chr4_2:67004047-67004066 Msa0616810:five_prime_UTR 50.0%
TCGCTTCCTGCAAACAAGGT+TGG + chr4_2:67004051-67004070 Msa0616810:five_prime_UTR 50.0%
! GAAGAAGAACCAGAGCGCTA+AGG + chr4_2:67004268-67004287 Msa0616810:CDS 50.0%
AAGTCTTCCCTCACGAGCAT+AGG - chr4_2:67004405-67004424 None:intergenic 50.0%
!!! TTTTGCAGCTCTGAGTCCAC+TGG + chr4_2:67004992-67005011 Msa0616810:intron 50.0%
TGTGATCCAATGGTGTCCAG+TGG - chr4_2:67005011-67005030 None:intergenic 50.0%
CTGGACACCATTGGATCACA+CGG + chr4_2:67005011-67005030 Msa0616810:CDS 50.0%
AACCAACGGAATGGCCAATG+CGG + chr4_2:67005073-67005092 Msa0616810:CDS 50.0%
TCTTCAAGCTCCCATTCCTG+CGG - chr4_2:67005121-67005140 None:intergenic 50.0%
ACTTGGCAGCTTAGGGTTCG+GGG - chr4_2:67003781-67003800 None:intergenic 55.0%
GACTTGGCAGCTTAGGGTTC+GGG - chr4_2:67003782-67003801 None:intergenic 55.0%
TTCTCGCATCCTTAGCGCTC+TGG - chr4_2:67004280-67004299 None:intergenic 55.0%
!! CGCTGGACCGATTCGATTTG+TGG + chr4_2:67004331-67004350 Msa0616810:CDS 55.0%
CTGAGTCCACTGGACACCAT+TGG + chr4_2:67005002-67005021 Msa0616810:CDS 55.0%
GACACCATTGGATCACACGG+AGG + chr4_2:67005014-67005033 Msa0616810:CDS 55.0%
AGCTCCTCCGTGTGATCCAA+TGG - chr4_2:67005021-67005040 None:intergenic 55.0%
GCCAGAGACAACCAACGGAA+TGG + chr4_2:67005064-67005083 Msa0616810:CDS 55.0%
! GCCATTCCGTTGGTTGTCTC+TGG - chr4_2:67005068-67005087 None:intergenic 55.0%
! ATCCGCATTGGCCATTCCGT+TGG - chr4_2:67005078-67005097 None:intergenic 55.0%
AACGGAATGGCCAATGCGGA+TGG + chr4_2:67005077-67005096 Msa0616810:CDS 55.0%
AATGGCCAATGCGGATGGAG+CGG + chr4_2:67005082-67005101 Msa0616810:CDS 55.0%
ACTTGGCAGCTTAGGGTTCG+GGG - chr4_2:67003781-67003800 None:intergenic 55.0%
GACTTGGCAGCTTAGGGTTC+GGG - chr4_2:67003782-67003801 None:intergenic 55.0%
TTCTCGCATCCTTAGCGCTC+TGG - chr4_2:67004280-67004299 None:intergenic 55.0%
!! CGCTGGACCGATTCGATTTG+TGG + chr4_2:67004331-67004350 Msa0616810:CDS 55.0%
CTGAGTCCACTGGACACCAT+TGG + chr4_2:67005002-67005021 Msa0616810:CDS 55.0%
GACACCATTGGATCACACGG+AGG + chr4_2:67005014-67005033 Msa0616810:CDS 55.0%
AGCTCCTCCGTGTGATCCAA+TGG - chr4_2:67005021-67005040 None:intergenic 55.0%
GCCAGAGACAACCAACGGAA+TGG + chr4_2:67005064-67005083 Msa0616810:CDS 55.0%
! GCCATTCCGTTGGTTGTCTC+TGG - chr4_2:67005068-67005087 None:intergenic 55.0%
! ATCCGCATTGGCCATTCCGT+TGG - chr4_2:67005078-67005097 None:intergenic 55.0%
AACGGAATGGCCAATGCGGA+TGG + chr4_2:67005077-67005096 Msa0616810:CDS 55.0%
AATGGCCAATGCGGATGGAG+CGG + chr4_2:67005082-67005101 Msa0616810:CDS 55.0%
! GGTTGGTACTACCGCTGCCT+CGG + chr4_2:67004068-67004087 Msa0616810:five_prime_UTR 60.0%
CGAGGGAGACCTTGTTGCCA+CGG + chr4_2:67004358-67004377 Msa0616810:CDS 60.0%
!! CATTGGCTCGCCGCAGGAAT+GGG + chr4_2:67005108-67005127 Msa0616810:CDS 60.0%
! GGTTGGTACTACCGCTGCCT+CGG + chr4_2:67004068-67004087 Msa0616810:five_prime_UTR 60.0%
CGAGGGAGACCTTGTTGCCA+CGG + chr4_2:67004358-67004377 Msa0616810:CDS 60.0%
!! CATTGGCTCGCCGCAGGAAT+GGG + chr4_2:67005108-67005127 Msa0616810:CDS 60.0%
TTGTTCAGGGGTCGACGCCG+AGG - chr4_2:67004088-67004107 None:intergenic 65.0%
TGCGACCGCTCCATCCGCAT+TGG - chr4_2:67005090-67005109 None:intergenic 65.0%
TGCGGATGGAGCGGTCGCAT+TGG + chr4_2:67005091-67005110 Msa0616810:CDS 65.0%
!! GCATTGGCTCGCCGCAGGAA+TGG + chr4_2:67005107-67005126 Msa0616810:CDS 65.0%
TTGTTCAGGGGTCGACGCCG+AGG - chr4_2:67004088-67004107 None:intergenic 65.0%
TGCGACCGCTCCATCCGCAT+TGG - chr4_2:67005090-67005109 None:intergenic 65.0%
TGCGGATGGAGCGGTCGCAT+TGG + chr4_2:67005091-67005110 Msa0616810:CDS 65.0%
!! GCATTGGCTCGCCGCAGGAA+TGG + chr4_2:67005107-67005126 Msa0616810:CDS 65.0%
AGGGGTCGACGCCGAGGCAG+CGG - chr4_2:67004082-67004101 None:intergenic 75.0%
!! CGGTCGCATTGGCTCGCCGC+AGG + chr4_2:67005102-67005121 Msa0616810:CDS 75.0%
AGGGGTCGACGCCGAGGCAG+CGG - chr4_2:67004082-67004101 None:intergenic 75.0%
!! CGGTCGCATTGGCTCGCCGC+AGG + chr4_2:67005102-67005121 Msa0616810:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 67003778 67005572 67003778 ID=Msa0616810;Name=Msa0616810
chr4_2 mRNA 67003778 67005572 67003778 ID=Msa0616810-mRNA-1;Parent=Msa0616810;Name=Msa0616810-mRNA-1;_AED=0.01;_eAED=0.01;_QI=473|1|1|1|1|1|2|385|140
chr4_2 exon 67003778 67004485 67003778 ID=Msa0616810-mRNA-1:exon:19092;Parent=Msa0616810-mRNA-1
chr4_2 exon 67005000 67005572 67005000 ID=Msa0616810-mRNA-1:exon:19093;Parent=Msa0616810-mRNA-1
chr4_2 five_prime_UTR 67003778 67004250 67003778 ID=Msa0616810-mRNA-1:five_prime_utr;Parent=Msa0616810-mRNA-1
chr4_2 CDS 67004251 67004485 67004251 ID=Msa0616810-mRNA-1:cds;Parent=Msa0616810-mRNA-1
chr4_2 CDS 67005000 67005187 67005000 ID=Msa0616810-mRNA-1:cds;Parent=Msa0616810-mRNA-1
chr4_2 three_prime_UTR 67005188 67005572 67005188 ID=Msa0616810-mRNA-1:three_prime_utr;Parent=Msa0616810-mRNA-1
Gene Sequence

>Msa0616810

ATGTTGTTGAGTAAGCAGAAGAAGAACCAGAGCGCTAAGGATGCGAGAAAGTTGTTGATCAACATCAATGTTTTGGGAAGCGCTGGACCGATTCGATTTGTGGTTAGCGAGGGAGACCTTGTTGCCACGGTTATTGATACTGCTCTCAAATCCTATGCTCGTGAGGGAAGACTTCCTATCTTAGGAAAGGATGTTACGAGTTTTGCTCTTTACTGTCAACACGTTGGATCTGATGCTCTGAGTCCACTGGACACCATTGGATCACACGGAGGAGCTAGAAACTTCATGCTATGCAAGAAGCCAGAGACAACCAACGGAATGGCCAATGCGGATGGAGCGGTCGCATTGGCTCGCCGCAGGAATGGGAGCTTGAAGAATTGGTTCAACAAATCTCTAAGTTCAGTTTATGTTCTCATTAGCTAG
Protein sequence

>Msa0616810

MLLSKQKKNQSAKDARKLLININVLGSAGPIRFVVSEGDLVATVIDTALKSYAREGRLPILGKDVTSFALYCQHVGSDALSPLDTIGSHGGARNFMLCKKPETTNGMANADGAVALARRRNGSLKNWFNKSLSSVYVLIS*