Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0621500 | Msa1162550 | -0.801304 | 9.591157e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGATTTGATGATCAAAA+AGG | 0.229452 | 4_2:+73559310 | Msa0621500:five_prime_UTR |
| ACGAAAGAAAATCAATTTAC+TGG | 0.343817 | 4_2:+73559447 | Msa0621500:CDS |
| GTATCTTTAGCTATCGAGTT+TGG | 0.354321 | 4_2:+73560070 | Msa0621500:CDS |
| GCTATCGAGTTTGGTGAACC+TGG | 0.462242 | 4_2:+73560079 | Msa0621500:CDS |
| GAATGGATGATTAAGCTTCA+TGG | 0.491117 | 4_2:-73559350 | None:intergenic |
| ACCTTCATATCACAAGTTCC+AGG | 0.526980 | 4_2:-73560097 | None:intergenic |
| ACTACAGATTCAGCGGAAGA+AGG | 0.548572 | 4_2:+73559700 | Msa0621500:intron |
| ACCTGGAACTTGTGATATGA+AGG | 0.591247 | 4_2:+73560096 | Msa0621500:CDS |
| TTATGAAACTACAGATTCAG+CGG | 0.641200 | 4_2:+73559693 | Msa0621500:intron |
| TGGAACTTGTGATATGAAGG+TGG | 0.701908 | 4_2:+73560099 | Msa0621500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATGTTATATAGAAACT+TGG | + | chr4_2:73559774-73559793 | Msa0621500:intron | 15.0% |
| !! | ATTAATGTTATATAGAAACT+TGG | + | chr4_2:73559774-73559793 | Msa0621500:intron | 15.0% |
| !!! | TTATGATTTGATGATCAAAA+AGG | + | chr4_2:73559310-73559329 | Msa0621500:five_prime_UTR | 20.0% |
| !!! | TATGATTTGATGATCAAAAA+GGG | + | chr4_2:73559311-73559330 | Msa0621500:five_prime_UTR | 20.0% |
| !!! | TTTTCTATAATTTCCTCAAA+AGG | - | chr4_2:73559648-73559667 | None:intergenic | 20.0% |
| !! | TATTGGAACATTCTTAATAT+TGG | - | chr4_2:73559844-73559863 | None:intergenic | 20.0% |
| !!! | TTATGATTTGATGATCAAAA+AGG | + | chr4_2:73559310-73559329 | Msa0621500:five_prime_UTR | 20.0% |
| !!! | TATGATTTGATGATCAAAAA+GGG | + | chr4_2:73559311-73559330 | Msa0621500:five_prime_UTR | 20.0% |
| !!! | TTTTCTATAATTTCCTCAAA+AGG | - | chr4_2:73559648-73559667 | None:intergenic | 20.0% |
| !! | TATTGGAACATTCTTAATAT+TGG | - | chr4_2:73559844-73559863 | None:intergenic | 20.0% |
| ! | ACGAAAGAAAATCAATTTAC+TGG | + | chr4_2:73559447-73559466 | Msa0621500:CDS | 25.0% |
| ! | TTGTTTCATAACACTTTGTT+TGG | + | chr4_2:73559558-73559577 | Msa0621500:intron | 25.0% |
| ! | ACGAAAGAAAATCAATTTAC+TGG | + | chr4_2:73559447-73559466 | Msa0621500:CDS | 25.0% |
| ! | TTGTTTCATAACACTTTGTT+TGG | + | chr4_2:73559558-73559577 | Msa0621500:intron | 25.0% |
| TCTCATCTGTAGTTAAGAAA+AGG | + | chr4_2:73559534-73559553 | Msa0621500:intron | 30.0% | |
| CTCATCTGTAGTTAAGAAAA+GGG | + | chr4_2:73559535-73559554 | Msa0621500:intron | 30.0% | |
| !!! | TGTTGATTTCATTCCTTTTG+AGG | + | chr4_2:73559632-73559651 | Msa0621500:intron | 30.0% |
| TTATGAAACTACAGATTCAG+CGG | + | chr4_2:73559693-73559712 | Msa0621500:intron | 30.0% | |
| ! | ATAGAAACTTGGAACTTGTT+TGG | + | chr4_2:73559785-73559804 | Msa0621500:intron | 30.0% |
| TGAAACACCAAAATCTTTCA+GGG | - | chr4_2:73559875-73559894 | None:intergenic | 30.0% | |
| CAACAATGACAATGTTGTAT+TGG | + | chr4_2:73560004-73560023 | Msa0621500:intron | 30.0% | |
| !! | AATGTTGTATTGGTTCAGAA+AGG | + | chr4_2:73560014-73560033 | Msa0621500:intron | 30.0% |
| TCTCATCTGTAGTTAAGAAA+AGG | + | chr4_2:73559534-73559553 | Msa0621500:intron | 30.0% | |
| CTCATCTGTAGTTAAGAAAA+GGG | + | chr4_2:73559535-73559554 | Msa0621500:intron | 30.0% | |
| !!! | TGTTGATTTCATTCCTTTTG+AGG | + | chr4_2:73559632-73559651 | Msa0621500:intron | 30.0% |
| TTATGAAACTACAGATTCAG+CGG | + | chr4_2:73559693-73559712 | Msa0621500:intron | 30.0% | |
| ! | ATAGAAACTTGGAACTTGTT+TGG | + | chr4_2:73559785-73559804 | Msa0621500:intron | 30.0% |
| TGAAACACCAAAATCTTTCA+GGG | - | chr4_2:73559875-73559894 | None:intergenic | 30.0% | |
| CAACAATGACAATGTTGTAT+TGG | + | chr4_2:73560004-73560023 | Msa0621500:intron | 30.0% | |
| !! | AATGTTGTATTGGTTCAGAA+AGG | + | chr4_2:73560014-73560033 | Msa0621500:intron | 30.0% |
| GAATGGATGATTAAGCTTCA+TGG | - | chr4_2:73559353-73559372 | None:intergenic | 35.0% | |
| ! | TCATCCATTCCCTTCTTTTT+CGG | + | chr4_2:73559363-73559382 | Msa0621500:CDS | 35.0% |
| GAAACACCAAAATCTTTCAG+GGG | - | chr4_2:73559874-73559893 | None:intergenic | 35.0% | |
| CTGAAACACCAAAATCTTTC+AGG | - | chr4_2:73559876-73559895 | None:intergenic | 35.0% | |
| GGTGTTTCAGAAAAGTCATA+AGG | + | chr4_2:73559886-73559905 | Msa0621500:intron | 35.0% | |
| TGTACAAGTGAGTGATAGAT+AGG | + | chr4_2:73559941-73559960 | Msa0621500:intron | 35.0% | |
| ATTACTAATCTAGATGACGC+AGG | - | chr4_2:73559974-73559993 | None:intergenic | 35.0% | |
| GTATCTTTAGCTATCGAGTT+TGG | + | chr4_2:73560070-73560089 | Msa0621500:CDS | 35.0% | |
| GAATGGATGATTAAGCTTCA+TGG | - | chr4_2:73559353-73559372 | None:intergenic | 35.0% | |
| ! | TCATCCATTCCCTTCTTTTT+CGG | + | chr4_2:73559363-73559382 | Msa0621500:CDS | 35.0% |
| GAAACACCAAAATCTTTCAG+GGG | - | chr4_2:73559874-73559893 | None:intergenic | 35.0% | |
| CTGAAACACCAAAATCTTTC+AGG | - | chr4_2:73559876-73559895 | None:intergenic | 35.0% | |
| GGTGTTTCAGAAAAGTCATA+AGG | + | chr4_2:73559886-73559905 | Msa0621500:intron | 35.0% | |
| TGTACAAGTGAGTGATAGAT+AGG | + | chr4_2:73559941-73559960 | Msa0621500:intron | 35.0% | |
| ATTACTAATCTAGATGACGC+AGG | - | chr4_2:73559974-73559993 | None:intergenic | 35.0% | |
| GTATCTTTAGCTATCGAGTT+TGG | + | chr4_2:73560070-73560089 | Msa0621500:CDS | 35.0% | |
| ! | CATCCATTCCCTTCTTTTTC+GGG | + | chr4_2:73559364-73559383 | Msa0621500:CDS | 40.0% |
| ! | ACAGATGAGAAGCGTAGAAA+AGG | - | chr4_2:73559524-73559543 | None:intergenic | 40.0% |
| !!! | GTCTGGCTTTTCAACAAATG+CGG | + | chr4_2:73559812-73559831 | Msa0621500:intron | 40.0% |
| !!! | TCTGGCTTTTCAACAAATGC+GGG | + | chr4_2:73559813-73559832 | Msa0621500:intron | 40.0% |
| ! | CTGTTACCCCTGAAAGATTT+TGG | + | chr4_2:73559865-73559884 | Msa0621500:intron | 40.0% |
| TCAAAACCAAACCTTCGTCA+TGG | + | chr4_2:73559910-73559929 | Msa0621500:intron | 40.0% | |
| ACCTGGAACTTGTGATATGA+AGG | + | chr4_2:73560096-73560115 | Msa0621500:CDS | 40.0% | |
| ACCTTCATATCACAAGTTCC+AGG | - | chr4_2:73560100-73560119 | None:intergenic | 40.0% | |
| TGGAACTTGTGATATGAAGG+TGG | + | chr4_2:73560099-73560118 | Msa0621500:CDS | 40.0% | |
| ! | CATCCATTCCCTTCTTTTTC+GGG | + | chr4_2:73559364-73559383 | Msa0621500:CDS | 40.0% |
| ! | ACAGATGAGAAGCGTAGAAA+AGG | - | chr4_2:73559524-73559543 | None:intergenic | 40.0% |
| !!! | GTCTGGCTTTTCAACAAATG+CGG | + | chr4_2:73559812-73559831 | Msa0621500:intron | 40.0% |
| !!! | TCTGGCTTTTCAACAAATGC+GGG | + | chr4_2:73559813-73559832 | Msa0621500:intron | 40.0% |
| ! | CTGTTACCCCTGAAAGATTT+TGG | + | chr4_2:73559865-73559884 | Msa0621500:intron | 40.0% |
| TCAAAACCAAACCTTCGTCA+TGG | + | chr4_2:73559910-73559929 | Msa0621500:intron | 40.0% | |
| ACCTGGAACTTGTGATATGA+AGG | + | chr4_2:73560096-73560115 | Msa0621500:CDS | 40.0% | |
| ACCTTCATATCACAAGTTCC+AGG | - | chr4_2:73560100-73560119 | None:intergenic | 40.0% | |
| TGGAACTTGTGATATGAAGG+TGG | + | chr4_2:73560099-73560118 | Msa0621500:CDS | 40.0% | |
| AGACCCGAAAAAGAAGGGAA+TGG | - | chr4_2:73559370-73559389 | None:intergenic | 45.0% | |
| CCACAAGACCCGAAAAAGAA+GGG | - | chr4_2:73559375-73559394 | None:intergenic | 45.0% | |
| ACCACAAGACCCGAAAAAGA+AGG | - | chr4_2:73559376-73559395 | None:intergenic | 45.0% | |
| ACTACAGATTCAGCGGAAGA+AGG | + | chr4_2:73559700-73559719 | Msa0621500:intron | 45.0% | |
| ! | GGAACTTGTTTGGCTATGTC+TGG | + | chr4_2:73559795-73559814 | Msa0621500:intron | 45.0% |
| CTTTCAGGGGTAACAGCTAT+TGG | - | chr4_2:73559861-73559880 | None:intergenic | 45.0% | |
| GGTTCAGAAAGGTCTTTAGC+TGG | + | chr4_2:73560025-73560044 | Msa0621500:intron | 45.0% | |
| AGACCCGAAAAAGAAGGGAA+TGG | - | chr4_2:73559370-73559389 | None:intergenic | 45.0% | |
| CCACAAGACCCGAAAAAGAA+GGG | - | chr4_2:73559375-73559394 | None:intergenic | 45.0% | |
| ACCACAAGACCCGAAAAAGA+AGG | - | chr4_2:73559376-73559395 | None:intergenic | 45.0% | |
| ACTACAGATTCAGCGGAAGA+AGG | + | chr4_2:73559700-73559719 | Msa0621500:intron | 45.0% | |
| ! | GGAACTTGTTTGGCTATGTC+TGG | + | chr4_2:73559795-73559814 | Msa0621500:intron | 45.0% |
| CTTTCAGGGGTAACAGCTAT+TGG | - | chr4_2:73559861-73559880 | None:intergenic | 45.0% | |
| GGTTCAGAAAGGTCTTTAGC+TGG | + | chr4_2:73560025-73560044 | Msa0621500:intron | 45.0% | |
| !!! | CCCTTCTTTTTCGGGTCTTG+TGG | + | chr4_2:73559372-73559391 | Msa0621500:CDS | 50.0% |
| ! | GCTATCGAGTTTGGTGAACC+TGG | + | chr4_2:73560079-73560098 | Msa0621500:CDS | 50.0% |
| !!! | CCCTTCTTTTTCGGGTCTTG+TGG | + | chr4_2:73559372-73559391 | Msa0621500:CDS | 50.0% |
| ! | GCTATCGAGTTTGGTGAACC+TGG | + | chr4_2:73560079-73560098 | Msa0621500:CDS | 50.0% |
| GACGTGCCATGACGAAGGTT+TGG | - | chr4_2:73559919-73559938 | None:intergenic | 55.0% | |
| ACAGTGACGTGCCATGACGA+AGG | - | chr4_2:73559924-73559943 | None:intergenic | 55.0% | |
| GACGTGCCATGACGAAGGTT+TGG | - | chr4_2:73559919-73559938 | None:intergenic | 55.0% | |
| ACAGTGACGTGCCATGACGA+AGG | - | chr4_2:73559924-73559943 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 73559223 | 73560126 | 73559223 | ID=Msa0621500;Name=Msa0621500 |
| chr4_2 | mRNA | 73559223 | 73560126 | 73559223 | ID=Msa0621500-mRNA-1;Parent=Msa0621500;Name=Msa0621500-mRNA-1;_AED=0.47;_eAED=0.47;_QI=129|0.5|0.33|1|0|0.66|3|0|76 |
| chr4_2 | exon | 73559223 | 73559468 | 73559223 | ID=Msa0621500-mRNA-1:exon:22128;Parent=Msa0621500-mRNA-1 |
| chr4_2 | exon | 73559707 | 73559760 | 73559707 | ID=Msa0621500-mRNA-1:exon:22129;Parent=Msa0621500-mRNA-1 |
| chr4_2 | exon | 73560067 | 73560126 | 73560067 | ID=Msa0621500-mRNA-1:exon:22130;Parent=Msa0621500-mRNA-1 |
| chr4_2 | five_prime_UTR | 73559223 | 73559351 | 73559223 | ID=Msa0621500-mRNA-1:five_prime_utr;Parent=Msa0621500-mRNA-1 |
| chr4_2 | CDS | 73559352 | 73559468 | 73559352 | ID=Msa0621500-mRNA-1:cds;Parent=Msa0621500-mRNA-1 |
| chr4_2 | CDS | 73559707 | 73559760 | 73559707 | ID=Msa0621500-mRNA-1:cds;Parent=Msa0621500-mRNA-1 |
| chr4_2 | CDS | 73560067 | 73560126 | 73560067 | ID=Msa0621500-mRNA-1:cds;Parent=Msa0621500-mRNA-1 |
| Gene Sequence |
| Protein sequence |
