AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0624870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0624870 MtrunA17_Chr5g0411541 94.737 114 6 0 1 114 212 325 2.31e-75 226
Msa0624870 MtrunA17_Chr3g0141091 67.619 105 33 1 10 114 234 337 1.49e-46 152
Msa0624870 MtrunA17_Chr3g0131691 41.346 104 51 4 14 111 383 482 2.63e-12 61.6
Msa0624870 MtrunA17_Chr7g0252041 36.634 101 61 2 14 111 373 473 2.68e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0624870 AT5G65640.1 68.269 104 33 0 11 114 248 351 2.75e-45 150
Msa0624870 AT5G10570.1 65.957 94 32 0 21 114 222 315 1.90e-37 129
Msa0624870 AT1G12860.1 39.286 84 50 1 29 111 365 448 1.45e-11 60.1
Msa0624870 AT3G26744.1 35.644 101 62 1 14 111 392 492 3.21e-11 58.9
Msa0624870 AT3G26744.4 35.644 101 62 1 14 111 392 492 3.21e-11 58.9
Msa0624870 AT3G26744.2 35.644 101 62 1 14 111 392 492 3.21e-11 58.9

Find 90 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACCAAAGCTAACTTATT+TGG 0.132124 4_3:+3646123 None:intergenic
TCTTCAATATCAATAATTAA+AGG 0.208328 4_3:-3646177 Msa0624870:three_prime_UTR
TGAATCACCTTGCTGATCTT+AGG 0.222682 4_3:+3648438 None:intergenic
AGGCTGTCCTTCAACCCTTT+TGG 0.223211 4_3:+3648536 None:intergenic
TTTAAAGCATGTGCATATTC+AGG 0.229476 4_3:+3645934 None:intergenic
AGTGATTGCTTTATCTCTTC+AGG 0.235636 4_3:+3646297 None:intergenic
TGGAATGAAGTTGAACAAAA+TGG 0.249862 4_3:+3649064 None:intergenic
CATAGATTTGAATGTCCTTA+TGG 0.286074 4_3:-3648779 Msa0624870:five_prime_UTR
TTATCAATGTACTACTAAAA+AGG 0.286143 4_3:+3646009 None:intergenic
ACAAATCAGATAAACCTTTC+AGG 0.295093 4_3:-3648055 Msa0624870:CDS
AAACCTTTCAGGAATTTCTA+AGG 0.299341 4_3:-3648044 Msa0624870:CDS
TTTACCACAACAAGAAAGTA+TGG 0.308343 4_3:-3648687 Msa0624870:five_prime_UTR
GTGAATGTCACAACTTTATA+AGG 0.308796 4_3:-3645843 Msa0624870:three_prime_UTR
TTCCCATGTGTTTAGGTAGA+TGG 0.318517 4_3:-3646339 Msa0624870:intron
TATCAATAATTAAAGGAATT+AGG 0.318963 4_3:-3646170 Msa0624870:three_prime_UTR
TCATCATACCCTTATCTTGA+TGG 0.320494 4_3:-3648746 Msa0624870:five_prime_UTR
CTTTCTTGTTGTGGTAAAAC+TGG 0.323789 4_3:+3648692 None:intergenic
AGATAAAGCAATCACTATTC+AGG 0.330105 4_3:-3646290 Msa0624870:CDS
TTTCTTACCATTACTTCATT+TGG 0.330225 4_3:+3648013 None:intergenic
CAAGAAAGTATGGAAGAGTT+TGG 0.335442 4_3:-3648677 Msa0624870:five_prime_UTR
GAGTCAAAGGTCCAACCATT+AGG 0.336189 4_3:+3648872 None:intergenic
ACAGCCTTCAAAGAATCTTA+TGG 0.358273 4_3:-3648520 Msa0624870:five_prime_UTR
ATGGAAGAGTTTGGATTTGT+TGG 0.361028 4_3:-3648668 Msa0624870:five_prime_UTR
CATTCAAATCTATGGTCTAA+TGG 0.372381 4_3:+3648788 None:intergenic
ACCCTATGTATGTTAAATAC+AGG 0.390422 4_3:+3646074 None:intergenic
CAGTAACAATCCTGGCTTTG+TGG 0.397442 4_3:+3647838 None:intergenic
GAATCACCTTGCTGATCTTA+GGG 0.398992 4_3:+3648439 None:intergenic
CATAAGGACATTCAAATCTA+TGG 0.410090 4_3:+3648780 None:intergenic
AACGAGTTACTACTCCCTAA+TGG 0.410425 4_3:-3648887 Msa0624870:five_prime_UTR
ATGGACAGGACATCAATTCT+AGG 0.415879 4_3:-3648151 Msa0624870:five_prime_UTR
ATCTGCTGTGCCACAAAGCC+AGG 0.421676 4_3:-3647848 Msa0624870:CDS
GCAAATGAAGGGTTTAAAGA+TGG 0.426120 4_3:+3648824 None:intergenic
CTATTCAGGAATGCAGGTTA+TGG 0.429722 4_3:-3646276 Msa0624870:CDS
GTGATTGCTTTATCTCTTCA+GGG 0.432133 4_3:+3646298 None:intergenic
TAGTAAGTTACAATAAGAAA+TGG 0.436128 4_3:-3648089 Msa0624870:exon
GAAGAAAGCGTTTGAATGAC+AGG 0.437266 4_3:-3648485 Msa0624870:five_prime_UTR
GATTCCAAATAAGTTAGCTT+TGG 0.441100 4_3:-3646127 Msa0624870:three_prime_UTR
GGAATGAAGTTGAACAAAAT+GGG 0.446885 4_3:+3649065 None:intergenic
TCATCCATAAGATTCTTTGA+AGG 0.448575 4_3:+3648516 None:intergenic
AGTCAAAGGTCCAACCATTA+GGG 0.456761 4_3:+3648873 None:intergenic
AAAATGGAACTTTCTCAACT+TGG 0.469304 4_3:-3648971 Msa0624870:five_prime_UTR
TATTCATAGTTCACAAAAGT+GGG 0.479190 4_3:-3649006 Msa0624870:five_prime_UTR
ATGACAGGCTTTCCATGCTT+AGG 0.479470 4_3:-3648470 Msa0624870:five_prime_UTR
GCCAAATCCAAAAGGGTTGA+AGG 0.482278 4_3:-3648543 Msa0624870:five_prime_UTR
CCCTGTATTTAACATACATA+GGG 0.490522 4_3:-3646075 Msa0624870:three_prime_UTR
ACTGAGAATCCATCAAGATA+AGG 0.498561 4_3:+3648737 None:intergenic
CTGAGAATCCATCAAGATAA+GGG 0.499837 4_3:+3648738 None:intergenic
ACATGAAAGAGCTTCTAGAA+AGG 0.506584 4_3:-3648114 Msa0624870:five_prime_UTR
AGATACTGATTCTTGTGTCC+TGG 0.512516 4_3:+3647867 None:intergenic
TTTAATTATTGATATTGAAG+AGG 0.512898 4_3:+3646179 None:intergenic
TCTTCCATACTTTCTTGTTG+TGG 0.521400 4_3:+3648683 None:intergenic
ACATGGAACACTTTATCCAC+TGG 0.522465 4_3:-3648914 Msa0624870:five_prime_UTR
CAATCACTATTCAGGAATGC+AGG 0.523750 4_3:-3646282 Msa0624870:CDS
AGATGTCTCTAGGAAAATGA+TGG 0.527614 4_3:-3646249 Msa0624870:exon
TAAGTTACAATAAGAAATGG+AGG 0.530314 4_3:-3648086 Msa0624870:exon
TTCAGGAATGCAGGTTATGG+TGG 0.533157 4_3:-3646273 Msa0624870:CDS
ATTAAAGCCAAATGAAGTAA+TGG 0.533491 4_3:-3648020 Msa0624870:CDS
TGATGTTGAGCGGAGAGACC+AGG 0.534371 4_3:-3647885 Msa0624870:CDS
AAAATCACTTTGAATCCACT+TGG 0.537566 4_3:+3645965 None:intergenic
AGAATCTTATGGATGAAAGA+AGG 0.538180 4_3:-3648509 Msa0624870:five_prime_UTR
ACCCTGTATTTAACATACAT+AGG 0.538761 4_3:-3646076 Msa0624870:three_prime_UTR
GATGATGCATCAGTTCCATA+AGG 0.540398 4_3:+3648764 None:intergenic
CCCTATGTATGTTAAATACA+GGG 0.541182 4_3:+3646075 None:intergenic
ATGTCACAACTTTATAAGGT+TGG 0.546930 4_3:-3645839 Msa0624870:three_prime_UTR
TTGTTGGAAGTGAGAACAAG+AGG 0.555475 4_3:-3648652 Msa0624870:five_prime_UTR
GCTGTTGACAGTAACAATCC+TGG 0.558322 4_3:+3647830 None:intergenic
TTATTCATAGTTCACAAAAG+TGG 0.563281 4_3:-3649007 Msa0624870:five_prime_UTR
TTATGGTGGGAGATGTCTCT+AGG 0.570179 4_3:-3646259 Msa0624870:exon
AGTTACTACTCCCTAATGGT+TGG 0.572860 4_3:-3648883 Msa0624870:five_prime_UTR
ATTGCAAGCATCTTGCTCAA+AGG 0.575987 4_3:-3647741 Msa0624870:intron
ATTCCTTAGAAATTCCTGAA+AGG 0.577724 4_3:+3648041 None:intergenic
GTTGAGAATGAAGCAAATGA+AGG 0.578645 4_3:+3648812 None:intergenic
GTTACAATAAGAAATGGAGG+AGG 0.581473 4_3:-3648083 Msa0624870:exon
TAAAATCAGCTGCAAAGTTG+AGG 0.582223 4_3:-3648618 Msa0624870:five_prime_UTR
GCGAACACATTAGAAGCATT+AGG 0.583837 4_3:-3647809 Msa0624870:CDS
GCCATTGCAACTTGATACAA+AGG 0.600246 4_3:+3645874 None:intergenic
TCCTTTGTATCAAGTTGCAA+TGG 0.600570 4_3:-3645875 Msa0624870:three_prime_UTR
TAACACACTGGTGAATCTCA+AGG 0.606173 4_3:+3647786 None:intergenic
TCAGGAATGCAGGTTATGGT+GGG 0.610095 4_3:-3646272 Msa0624870:CDS
TTGAGAATGAAGCAAATGAA+GGG 0.614806 4_3:+3648813 None:intergenic
TAGTAACTCGTTCAAACCAG+TGG 0.618072 4_3:+3648898 None:intergenic
AATGGTAAGAAATTCCCCTA+AGG 0.619045 4_3:-3648002 Msa0624870:intron
CAATAAGAAATGGAGGAGGA+AGG 0.620297 4_3:-3648079 Msa0624870:exon
TGAAACTGCTTATAACACAC+TGG 0.622427 4_3:+3647774 None:intergenic
GGTGCTGAATCATCAAACTC+AGG 0.624297 4_3:+3648713 None:intergenic
AGGGACAATTGACCTAAGCA+TGG 0.627542 4_3:+3648458 None:intergenic
AAACAGAAAAGTCTTAACAA+TGG 0.636764 4_3:+3649044 None:intergenic
GGAACTAAACCATACCTTAG+GGG 0.677566 4_3:+3647988 None:intergenic
TAGTTCCAAGAAAAGACACA+TGG 0.690258 4_3:-3648931 Msa0624870:five_prime_UTR
AATTGTCCCTAAGATCAGCA+AGG 0.709185 4_3:-3648445 Msa0624870:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTGAATAAATTTAATTAA+TGG - chr4_3:3647024-3647043 Msa0624870:intron 10.0%
!!! TTTAATAAATTGAATTTTGA+TGG - chr4_3:3647676-3647695 Msa0624870:intron 10.0%
!!! TTTTAATTTTAAGGATTTTA+TGG + chr4_3:3648094-3648113 None:intergenic 10.0%
!!! TACTATTGATTTTAATTTTA+AGG + chr4_3:3648103-3648122 None:intergenic 10.0%
!!! ATAGTAAAAGAATTTTTTAT+TGG - chr4_3:3648117-3648136 Msa0624870:five_prime_UTR 10.0%
!!! AAAATGTAAATTTTGTAAGA+TGG - chr4_3:3646787-3646806 Msa0624870:intron 15.0%
!! ATTAAATTTATTCAGAAACT+TGG + chr4_3:3647021-3647040 None:intergenic 15.0%
!!! GTTTTTTTTCTCTATAATTA+GGG - chr4_3:3647427-3647446 Msa0624870:intron 15.0%
!!! TTTTTTTTCTCTATAATTAG+GGG - chr4_3:3647428-3647447 Msa0624870:intron 15.0%
!! TTATATTTAAAAAAGTCTTG+AGG - chr4_3:3647580-3647599 Msa0624870:intron 15.0%
!!! TTTTTTTTGAAAGATTTGTA+AGG - chr4_3:3647947-3647966 Msa0624870:intron 15.0%
!!! TTTTTTTGAAAGATTTGTAA+GGG - chr4_3:3647948-3647967 Msa0624870:intron 15.0%
!! TAACAAATAAAAAATGTGTA+TGG - chr4_3:3648019-3648038 Msa0624870:CDS 15.0%
!!! TATGGGATTTTAAAATTTTT+AGG + chr4_3:3648240-3648259 None:intergenic 15.0%
!! TTTAATTATTGATATTGAAG+AGG + chr4_3:3648781-3648800 None:intergenic 15.0%
!! TCTTCAATATCAATAATTAA+AGG - chr4_3:3648780-3648799 Msa0624870:five_prime_UTR 15.0%
!! TATCAATAATTAAAGGAATT+AGG - chr4_3:3648787-3648806 Msa0624870:five_prime_UTR 15.0%
!!! ATGCTTTAAAATTTCTTTTT+TGG - chr4_3:3649036-3649055 Msa0624870:five_prime_UTR 15.0%
!!! TGCTTTAAAATTTCTTTTTT+GGG - chr4_3:3649037-3649056 Msa0624870:five_prime_UTR 15.0%
!!! ATGTATGTGTGTTTTATTTT+TGG - chr4_3:3646669-3646688 Msa0624870:intron 20.0%
!! TAGTAAGTTACAATAAGAAA+TGG - chr4_3:3646868-3646887 Msa0624870:intron 20.0%
!!! CGTTTTTTTTCTCTATAATT+AGG - chr4_3:3647426-3647445 Msa0624870:intron 20.0%
!!! TTTTTTTCTCTATAATTAGG+GGG - chr4_3:3647429-3647448 Msa0624870:intron 20.0%
!! TTAATTATGAAAAAGTCTTG+TGG - chr4_3:3647492-3647511 Msa0624870:intron 20.0%
!! CAAAATCATTTAAATTTCGA+AGG - chr4_3:3647649-3647668 Msa0624870:intron 20.0%
!!! TTCAAAAAAAATTCTAAGAG+AGG + chr4_3:3647938-3647957 None:intergenic 20.0%
!!! AAATGTACAATTGAATTTTG+TGG - chr4_3:3647985-3648004 Msa0624870:intron 20.0%
!!! AATGTACAATTGAATTTTGT+GGG - chr4_3:3647986-3648005 Msa0624870:intron 20.0%
!!! TGGATTTTACAAGATTTTAA+GGG + chr4_3:3648074-3648093 None:intergenic 20.0%
!!! ATGGATTTTACAAGATTTTA+AGG + chr4_3:3648075-3648094 None:intergenic 20.0%
!!! TTTAGGATTCAATGATTTTA+GGG + chr4_3:3648223-3648242 None:intergenic 20.0%
!!! TTTTAGGATTCAATGATTTT+AGG + chr4_3:3648224-3648243 None:intergenic 20.0%
!!! GATTTATACACTGAATTTTA+AGG + chr4_3:3648277-3648296 None:intergenic 20.0%
!! TTATCAATGTACTACTAAAA+AGG + chr4_3:3648951-3648970 None:intergenic 20.0%
!!! TTTTAGTAGTACATTGATAA+TGG - chr4_3:3648951-3648970 Msa0624870:five_prime_UTR 20.0%
! AAACAGAAAAGTCTTAACAA+TGG + chr4_3:3645916-3645935 None:intergenic 25.0%
!! ATTGTTAAGACTTTTCTGTT+TGG - chr4_3:3645915-3645934 Msa0624870:three_prime_UTR 25.0%
! TTATTCATAGTTCACAAAAG+TGG - chr4_3:3645950-3645969 Msa0624870:three_prime_UTR 25.0%
! TATTCATAGTTCACAAAAGT+GGG - chr4_3:3645951-3645970 Msa0624870:three_prime_UTR 25.0%
!! ATTTTCATCAAATGAGTCAA+AGG + chr4_3:3646101-3646120 None:intergenic 25.0%
! TAAGTTACAATAAGAAATGG+AGG - chr4_3:3646871-3646890 Msa0624870:intron 25.0%
! ATTAAAGCCAAATGAAGTAA+TGG - chr4_3:3646937-3646956 Msa0624870:intron 25.0%
! TTTCTTACCATTACTTCATT+TGG + chr4_3:3646947-3646966 None:intergenic 25.0%
! GATTATGCATTAATTCTTCA+AGG + chr4_3:3646993-3647012 None:intergenic 25.0%
! AAATTGTATTACTGCATGTT+CGG - chr4_3:3647244-3647263 Msa0624870:intron 25.0%
!!! AAGCGTAGTTTTTAGTAAAA+TGG + chr4_3:3647308-3647327 None:intergenic 25.0%
! ATCACTTAAAAAAAGTCTCA+TGG - chr4_3:3647622-3647641 Msa0624870:intron 25.0%
!!! TTGAATTTTGATGGATTTTG+TGG - chr4_3:3647685-3647704 Msa0624870:intron 25.0%
!!! TGAATTTTGATGGATTTTGT+GGG - chr4_3:3647686-3647705 Msa0624870:intron 25.0%
! ATGAAAAGATGAGATTTCTT+GGG - chr4_3:3647745-3647764 Msa0624870:CDS 25.0%
!! TTAGGATTCAATGATTTTAG+GGG + chr4_3:3648222-3648241 None:intergenic 25.0%
!! AGGATTTCAAAGGATTTTAT+GGG + chr4_3:3648257-3648276 None:intergenic 25.0%
!! AAGGATTTCAAAGGATTTTA+TGG + chr4_3:3648258-3648277 None:intergenic 25.0%
!!! CTGAATTTTAAGGATTTCAA+AGG + chr4_3:3648267-3648286 None:intergenic 25.0%
! AATCGAATGTAATAAATTCC+TGG + chr4_3:3648457-3648476 None:intergenic 25.0%
! TTTAACCATGCAAACAATTA+TGG + chr4_3:3648566-3648585 None:intergenic 25.0%
! TAAACCAAAGCTAACTTATT+TGG + chr4_3:3648837-3648856 None:intergenic 25.0%
GGAATGAAGTTGAACAAAAT+GGG + chr4_3:3645895-3645914 None:intergenic 30.0%
TGGAATGAAGTTGAACAAAA+TGG + chr4_3:3645896-3645915 None:intergenic 30.0%
TGGGAAAAGAGAAAGAAAAA+TGG - chr4_3:3645970-3645989 Msa0624870:three_prime_UTR 30.0%
AAAATGGAACTTTCTCAACT+TGG - chr4_3:3645986-3646005 Msa0624870:three_prime_UTR 30.0%
TTGAGAATGAAGCAAATGAA+GGG + chr4_3:3646147-3646166 None:intergenic 30.0%
CATTCAAATCTATGGTCTAA+TGG + chr4_3:3646172-3646191 None:intergenic 30.0%
CATAAGGACATTCAAATCTA+TGG + chr4_3:3646180-3646199 None:intergenic 30.0%
CATAGATTTGAATGTCCTTA+TGG - chr4_3:3646178-3646197 Msa0624870:three_prime_UTR 30.0%
TTTACCACAACAAGAAAGTA+TGG - chr4_3:3646270-3646289 Msa0624870:CDS 30.0%
! TGAAACTCCAGTTTTTAACA+TGG - chr4_3:3646372-3646391 Msa0624870:intron 30.0%
! GAAACTCCAGTTTTTAACAT+GGG - chr4_3:3646373-3646392 Msa0624870:intron 30.0%
! TTTTTAACATGGGTTTGTGT+GGG - chr4_3:3646383-3646402 Msa0624870:intron 30.0%
AGAAAAAAGCCAAATCCAAA+AGG - chr4_3:3646406-3646425 Msa0624870:intron 30.0%
GAAAAAAGCCAAATCCAAAA+GGG - chr4_3:3646407-3646426 Msa0624870:intron 30.0%
TCATCCATAAGATTCTTTGA+AGG + chr4_3:3646444-3646463 None:intergenic 30.0%
AGAATCTTATGGATGAAAGA+AGG - chr4_3:3646448-3646467 Msa0624870:intron 30.0%
! ATATCTTCAATCTGAAGTGT+CGG + chr4_3:3646597-3646616 None:intergenic 30.0%
!!! TTCAGTTATTTTTTTGTGCC+TGG - chr4_3:3646618-3646637 Msa0624870:intron 30.0%
TAGTCAATTGTAGTCAATGA+AGG + chr4_3:3646716-3646735 None:intergenic 30.0%
AATTGACTAGCACTTGTATT+TGG - chr4_3:3646727-3646746 Msa0624870:intron 30.0%
! GTAAATTTTGTAAGATGGAC+AGG - chr4_3:3646792-3646811 Msa0624870:intron 30.0%
ACAAATCAGATAAACCTTTC+AGG - chr4_3:3646902-3646921 Msa0624870:intron 30.0%
AAACCTTTCAGGAATTTCTA+AGG - chr4_3:3646913-3646932 Msa0624870:intron 30.0%
ATTCCTTAGAAATTCCTGAA+AGG + chr4_3:3646919-3646938 None:intergenic 30.0%
!! TTTTTCAGTTTGATGTTGAG+CGG - chr4_3:3647062-3647081 Msa0624870:intron 30.0%
!!! CGTAGTTTTTAGTAAAATGG+CGG + chr4_3:3647305-3647324 None:intergenic 30.0%
! TGTATTGGATTAGGATTCTA+TGG - chr4_3:3647453-3647472 Msa0624870:intron 30.0%
! GTATTGGATTAGGATTCTAT+GGG - chr4_3:3647454-3647473 Msa0624870:intron 30.0%
GCAACTTAAAAAAAGTCTTG+TGG - chr4_3:3647533-3647552 Msa0624870:intron 30.0%
CATGAAAAGATGAGATTTCT+TGG - chr4_3:3647744-3647763 Msa0624870:CDS 30.0%
AATTCCTGGCGAAAAATTAA+GGG + chr4_3:3648443-3648462 None:intergenic 30.0%
AAATTCCTGGCGAAAAATTA+AGG + chr4_3:3648444-3648463 None:intergenic 30.0%
CATATTATTCCCATGTGTTT+AGG - chr4_3:3648611-3648630 Msa0624870:five_prime_UTR 30.0%
AGATAAAGCAATCACTATTC+AGG - chr4_3:3648667-3648686 Msa0624870:five_prime_UTR 30.0%
ATTTGCAGATGATGAAAAAG+AGG - chr4_3:3648746-3648765 Msa0624870:five_prime_UTR 30.0%
! GATTCCAAATAAGTTAGCTT+TGG - chr4_3:3648830-3648849 Msa0624870:five_prime_UTR 30.0%
ACCCTGTATTTAACATACAT+AGG - chr4_3:3648881-3648900 Msa0624870:five_prime_UTR 30.0%
CCCTATGTATGTTAAATACA+GGG + chr4_3:3648885-3648904 None:intergenic 30.0%
CCCTGTATTTAACATACATA+GGG - chr4_3:3648882-3648901 Msa0624870:five_prime_UTR 30.0%
ACCCTATGTATGTTAAATAC+AGG + chr4_3:3648886-3648905 None:intergenic 30.0%
! AGGTGGAATTTTATCACAAT+GGG + chr4_3:3648931-3648950 None:intergenic 30.0%
! AAGGTGGAATTTTATCACAA+TGG + chr4_3:3648932-3648951 None:intergenic 30.0%
TCAATGTACTACTAAAAAGG+TGG + chr4_3:3648948-3648967 None:intergenic 30.0%
! TTTGAATCCACTTGGATTTT+TGG + chr4_3:3648987-3649006 None:intergenic 30.0%
AAAATCACTTTGAATCCACT+TGG + chr4_3:3648995-3649014 None:intergenic 30.0%
! TTTAAAGCATGTGCATATTC+AGG + chr4_3:3649026-3649045 None:intergenic 30.0%
GTGAATGTCACAACTTTATA+AGG - chr4_3:3649114-3649133 Msa0624870:five_prime_UTR 30.0%
!! TTCTCAACTTGGTTTTCTTG+AGG - chr4_3:3645997-3646016 Msa0624870:three_prime_UTR 35.0%
TAGTTCCAAGAAAAGACACA+TGG - chr4_3:3646026-3646045 Msa0624870:three_prime_UTR 35.0%
GCAAATGAAGGGTTTAAAGA+TGG + chr4_3:3646136-3646155 None:intergenic 35.0%
GTTGAGAATGAAGCAAATGA+AGG + chr4_3:3646148-3646167 None:intergenic 35.0%
TCATCATACCCTTATCTTGA+TGG - chr4_3:3646211-3646230 Msa0624870:three_prime_UTR 35.0%
CTGAGAATCCATCAAGATAA+GGG + chr4_3:3646222-3646241 None:intergenic 35.0%
ACTGAGAATCCATCAAGATA+AGG + chr4_3:3646223-3646242 None:intergenic 35.0%
! CTTTCTTGTTGTGGTAAAAC+TGG + chr4_3:3646268-3646287 None:intergenic 35.0%
TCTTCCATACTTTCTTGTTG+TGG + chr4_3:3646277-3646296 None:intergenic 35.0%
! CAAGAAAGTATGGAAGAGTT+TGG - chr4_3:3646280-3646299 Msa0624870:CDS 35.0%
ATGGAAGAGTTTGGATTTGT+TGG - chr4_3:3646289-3646308 Msa0624870:CDS 35.0%
TAAAATCAGCTGCAAAGTTG+AGG - chr4_3:3646339-3646358 Msa0624870:intron 35.0%
CACAAACCCATGTTAAAAAC+TGG + chr4_3:3646382-3646401 None:intergenic 35.0%
! GTTTTTAACATGGGTTTGTG+TGG - chr4_3:3646382-3646401 Msa0624870:intron 35.0%
ACAGCCTTCAAAGAATCTTA+TGG - chr4_3:3646437-3646456 Msa0624870:intron 35.0%
GTGTGTATGTTTATGTTGTC+TGG - chr4_3:3646569-3646588 Msa0624870:intron 35.0%
! ACATGAAAGAGCTTCTAGAA+AGG - chr4_3:3646843-3646862 Msa0624870:intron 35.0%
GTTACAATAAGAAATGGAGG+AGG - chr4_3:3646874-3646893 Msa0624870:intron 35.0%
AATGGTAAGAAATTCCCCTA+AGG - chr4_3:3646955-3646974 Msa0624870:intron 35.0%
TAAGAAATTCCCCTAAGGTA+TGG - chr4_3:3646960-3646979 Msa0624870:intron 35.0%
AGGAACTAAACCATACCTTA+GGG + chr4_3:3646973-3646992 None:intergenic 35.0%
AAGGAACTAAACCATACCTT+AGG + chr4_3:3646974-3646993 None:intergenic 35.0%
TGAAACTGCTTATAACACAC+TGG + chr4_3:3647186-3647205 None:intergenic 35.0%
! AACTACGCTTTTGAGCTTTA+AGG - chr4_3:3647318-3647337 Msa0624870:intron 35.0%
!! ACTACGCTTTTGAGCTTTAA+GGG - chr4_3:3647319-3647338 Msa0624870:intron 35.0%
CTATAATTAGGGGGATGTAT+TGG - chr4_3:3647438-3647457 Msa0624870:intron 35.0%
! AAAAATTAAGGGGGGTGTAT+TGG + chr4_3:3648432-3648451 None:intergenic 35.0%
ATTCCTGGCGAAAAATTAAG+GGG + chr4_3:3648442-3648461 None:intergenic 35.0%
!!! CTAGTCTATTTTTGGTTCTG+CGG + chr4_3:3648537-3648556 None:intergenic 35.0%
AACCATGCAAACAATTATGG+AGG + chr4_3:3648563-3648582 None:intergenic 35.0%
GTGATTGCTTTATCTCTTCA+GGG + chr4_3:3648662-3648681 None:intergenic 35.0%
AGTGATTGCTTTATCTCTTC+AGG + chr4_3:3648663-3648682 None:intergenic 35.0%
! AGATGTCTCTAGGAAAATGA+TGG - chr4_3:3648708-3648727 Msa0624870:five_prime_UTR 35.0%
! TCCTTTGTATCAAGTTGCAA+TGG - chr4_3:3649082-3649101 Msa0624870:five_prime_UTR 35.0%
! GTGTTCCATGTGTCTTTTCT+TGG + chr4_3:3646034-3646053 None:intergenic 40.0%
ACATGGAACACTTTATCCAC+TGG - chr4_3:3646043-3646062 Msa0624870:three_prime_UTR 40.0%
TAGTAACTCGTTCAAACCAG+TGG + chr4_3:3646062-3646081 None:intergenic 40.0%
AACGAGTTACTACTCCCTAA+TGG - chr4_3:3646070-3646089 Msa0624870:three_prime_UTR 40.0%
AGTTACTACTCCCTAATGGT+TGG - chr4_3:3646074-3646093 Msa0624870:three_prime_UTR 40.0%
AGTCAAAGGTCCAACCATTA+GGG + chr4_3:3646087-3646106 None:intergenic 40.0%
GATGATGCATCAGTTCCATA+AGG + chr4_3:3646196-3646215 None:intergenic 40.0%
TTGTTGGAAGTGAGAACAAG+AGG - chr4_3:3646305-3646324 Msa0624870:CDS 40.0%
! TCCTTCAACCCTTTTGGATT+TGG + chr4_3:3646418-3646437 None:intergenic 40.0%
!! GAAGAAAGCGTTTGAATGAC+AGG - chr4_3:3646472-3646491 Msa0624870:intron 40.0%
AATTGTCCCTAAGATCAGCA+AGG - chr4_3:3646512-3646531 Msa0624870:intron 40.0%
GAATCACCTTGCTGATCTTA+GGG + chr4_3:3646521-3646540 None:intergenic 40.0%
TGAATCACCTTGCTGATCTT+AGG + chr4_3:3646522-3646541 None:intergenic 40.0%
TGAGCACTTACATAAACACC+AGG + chr4_3:3646639-3646658 None:intergenic 40.0%
ATGGACAGGACATCAATTCT+AGG - chr4_3:3646806-3646825 Msa0624870:intron 40.0%
CAATAAGAAATGGAGGAGGA+AGG - chr4_3:3646878-3646897 Msa0624870:intron 40.0%
GGAACTAAACCATACCTTAG+GGG + chr4_3:3646972-3646991 None:intergenic 40.0%
!! AGATACTGATTCTTGTGTCC+TGG + chr4_3:3647093-3647112 None:intergenic 40.0%
!! GCGAACACATTAGAAGCATT+AGG - chr4_3:3647148-3647167 Msa0624870:intron 40.0%
TAACACACTGGTGAATCTCA+AGG + chr4_3:3647174-3647193 None:intergenic 40.0%
ATTGCAAGCATCTTGCTCAA+AGG - chr4_3:3647216-3647235 Msa0624870:intron 40.0%
TTAGGGGGATGTATTGGATT+AGG - chr4_3:3647444-3647463 Msa0624870:intron 40.0%
ATAGAGAGACGAAGAGAGAA+AGG + chr4_3:3647836-3647855 None:intergenic 40.0%
AGAGAGAGAGAGAGAGAAAA+TGG + chr4_3:3647863-3647882 None:intergenic 40.0%
TTCCTGGCGAAAAATTAAGG+GGG + chr4_3:3648441-3648460 None:intergenic 40.0%
!! GGAGGCAACTAGTCTATTTT+TGG + chr4_3:3648545-3648564 None:intergenic 40.0%
TGCCTCCATAATTGTTTGCA+TGG - chr4_3:3648558-3648577 Msa0624870:five_prime_UTR 40.0%
TTCCCATGTGTTTAGGTAGA+TGG - chr4_3:3648618-3648637 Msa0624870:five_prime_UTR 40.0%
GACCATCTACCTAAACACAT+GGG + chr4_3:3648623-3648642 None:intergenic 40.0%
TGACCATCTACCTAAACACA+TGG + chr4_3:3648624-3648643 None:intergenic 40.0%
CAATCACTATTCAGGAATGC+AGG - chr4_3:3648675-3648694 Msa0624870:five_prime_UTR 40.0%
CTATTCAGGAATGCAGGTTA+TGG - chr4_3:3648681-3648700 Msa0624870:five_prime_UTR 40.0%
GTTGTTGCCAAAAATCCAAG+TGG - chr4_3:3648977-3648996 Msa0624870:five_prime_UTR 40.0%
GCCATTGCAACTTGATACAA+AGG + chr4_3:3649086-3649105 None:intergenic 40.0%
GAGTCAAAGGTCCAACCATT+AGG + chr4_3:3646088-3646107 None:intergenic 45.0%
! GGTGCTGAATCATCAAACTC+AGG + chr4_3:3646247-3646266 None:intergenic 45.0%
GCCAAATCCAAAAGGGTTGA+AGG - chr4_3:3646414-3646433 Msa0624870:intron 45.0%
ATGACAGGCTTTCCATGCTT+AGG - chr4_3:3646487-3646506 Msa0624870:intron 45.0%
AGGGACAATTGACCTAAGCA+TGG + chr4_3:3646502-3646521 None:intergenic 45.0%
!! CAGTAACAATCCTGGCTTTG+TGG + chr4_3:3647122-3647141 None:intergenic 45.0%
GCTGTTGACAGTAACAATCC+TGG + chr4_3:3647130-3647149 None:intergenic 45.0%
ACGAAGAGAGAAAGGAGAGA+GGG + chr4_3:3647828-3647847 None:intergenic 45.0%
GTGAGAGAGAAGAAAGAGGA+GGG + chr4_3:3647916-3647935 None:intergenic 45.0%
AGAGGTGAGAGAGAAGAAAG+AGG + chr4_3:3647920-3647939 None:intergenic 45.0%
! TAAGGGGGGTGTATTGGATT+AGG + chr4_3:3648426-3648445 None:intergenic 45.0%
TCCTGGCGAAAAATTAAGGG+GGG + chr4_3:3648440-3648459 None:intergenic 45.0%
TTCAGGAATGCAGGTTATGG+TGG - chr4_3:3648684-3648703 Msa0624870:five_prime_UTR 45.0%
TCAGGAATGCAGGTTATGGT+GGG - chr4_3:3648685-3648704 Msa0624870:five_prime_UTR 45.0%
! TTATGGTGGGAGATGTCTCT+AGG - chr4_3:3648698-3648717 Msa0624870:five_prime_UTR 45.0%
! AGGCTGTCCTTCAACCCTTT+TGG + chr4_3:3646424-3646443 None:intergenic 50.0%
AAGAGAGAAAGGAGAGAGGG+GGG + chr4_3:3647825-3647844 None:intergenic 50.0%
GAAGAGAGAAAGGAGAGAGG+GGG + chr4_3:3647826-3647845 None:intergenic 50.0%
CGAAGAGAGAAAGGAGAGAG+GGG + chr4_3:3647827-3647846 None:intergenic 50.0%
GACGAAGAGAGAAAGGAGAG+AGG + chr4_3:3647829-3647848 None:intergenic 50.0%
GAGAAGAAAGAGGAGGGAGA+GGG + chr4_3:3647910-3647929 None:intergenic 50.0%
AGAGAAGAAAGAGGAGGGAG+AGG + chr4_3:3647911-3647930 None:intergenic 50.0%
GGTGAGAGAGAAGAAAGAGG+AGG + chr4_3:3647917-3647936 None:intergenic 50.0%
! ACCCCCCTTAATTTTTCGCC+AGG - chr4_3:3648436-3648455 Msa0624870:intron 50.0%
TGATGTTGAGCGGAGAGACC+AGG - chr4_3:3647072-3647091 Msa0624870:intron 55.0%
ATCTGCTGTGCCACAAAGCC+AGG - chr4_3:3647109-3647128 Msa0624870:intron 55.0%
AGAAAGAGGAGGGAGAGGGA+GGG + chr4_3:3647906-3647925 None:intergenic 55.0%
AAGAAAGAGGAGGGAGAGGG+AGG + chr4_3:3647907-3647926 None:intergenic 55.0%
AGAAAGGAGAGAGGGGGGAG+GGG + chr4_3:3647820-3647839 None:intergenic 60.0%
GAGAAAGGAGAGAGGGGGGA+GGG + chr4_3:3647821-3647840 None:intergenic 60.0%
AGAGAAAGGAGAGAGGGGGG+AGG + chr4_3:3647822-3647841 None:intergenic 60.0%
GGGGGAGGGGAGAGAGAGAA+GGG + chr4_3:3647807-3647826 None:intergenic 65.0%
AGAGGAGGGAGAGGGAGGGA+GGG + chr4_3:3647902-3647921 None:intergenic 65.0%
AAGAGGAGGGAGAGGGAGGG+AGG + chr4_3:3647903-3647922 None:intergenic 65.0%
GGGGGGAGGGGAGAGAGAGA+AGG + chr4_3:3647808-3647827 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 3645839 3649140 3645839 ID=Msa0624870;Name=Msa0624870
chr4_3 mRNA 3645839 3649140 3645839 ID=Msa0624870-mRNA-1;Parent=Msa0624870;Name=Msa0624870-mRNA-1;_AED=0.06;_eAED=0.06;_QI=776|1|1|1|0.66|0.5|4|421|114
chr4_3 exon 3645839 3646346 3645839 ID=Msa0624870-mRNA-1:exon:867;Parent=Msa0624870-mRNA-1
chr4_3 exon 3647742 3647909 3647742 ID=Msa0624870-mRNA-1:exon:866;Parent=Msa0624870-mRNA-1
chr4_3 exon 3648003 3648173 3648003 ID=Msa0624870-mRNA-1:exon:865;Parent=Msa0624870-mRNA-1
chr4_3 exon 3648446 3649140 3648446 ID=Msa0624870-mRNA-1:exon:864;Parent=Msa0624870-mRNA-1
chr4_3 five_prime_UTR 3648446 3649140 3648446 ID=Msa0624870-mRNA-1:five_prime_utr;Parent=Msa0624870-mRNA-1
chr4_3 five_prime_UTR 3648093 3648173 3648093 ID=Msa0624870-mRNA-1:five_prime_utr;Parent=Msa0624870-mRNA-1
chr4_3 CDS 3648003 3648092 3648003 ID=Msa0624870-mRNA-1:cds;Parent=Msa0624870-mRNA-1
chr4_3 CDS 3647742 3647909 3647742 ID=Msa0624870-mRNA-1:cds;Parent=Msa0624870-mRNA-1
chr4_3 CDS 3646260 3646346 3646260 ID=Msa0624870-mRNA-1:cds;Parent=Msa0624870-mRNA-1
chr4_3 three_prime_UTR 3645839 3646259 3645839 ID=Msa0624870-mRNA-1:three_prime_utr;Parent=Msa0624870-mRNA-1
Gene Sequence

>Msa0624870

ATGGAGGAGGAAGGAACAAATCAGATAAACCTTTCAGGAATTTCTAAGGAATTAAAGCCAAATGAAGTAATGGTAAGAAATTCCCCTAAGTTTGATGTTGAGCGGAGAGACCAGGACACAAGAATCAGTATCTGCTGTGCCACAAAGCCAGGATTGTTACTGTCAACAGCGAACACATTAGAAGCATTAGGCCTTGAGATTCACCAGTGTGTTATAAGCAGTTTCAATGATTTTTCATTGCAAGCATCTTGCTCAAAGGTAGATGGTCAGAGAAATTGTATGAACCCTGAAGAGATAAAGCAATCACTATTCAGGAATGCAGGTTATGGTGGGAGATGTCTCTAG
Protein sequence

>Msa0624870

MEEEGTNQINLSGISKELKPNEVMVRNSPKFDVERRDQDTRISICCATKPGLLLSTANTLEALGLEIHQCVISSFNDFSLQASCSKVDGQRNCMNPEEIKQSLFRNAGYGGRCL*