AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0590320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590320 MtrunA17_Chr5g0411541 94.737 114 6 0 1 114 212 325 2.31e-75 226
Msa0590320 MtrunA17_Chr3g0141091 67.619 105 33 1 10 114 234 337 1.49e-46 152
Msa0590320 MtrunA17_Chr3g0131691 41.346 104 51 4 14 111 383 482 2.63e-12 61.6
Msa0590320 MtrunA17_Chr7g0252041 36.634 101 61 2 14 111 373 473 2.68e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0590320 AT5G65640.1 68.269 104 33 0 11 114 248 351 2.75e-45 150
Msa0590320 AT5G10570.1 65.957 94 32 0 21 114 222 315 1.90e-37 129
Msa0590320 AT1G12860.1 39.286 84 50 1 29 111 365 448 1.45e-11 60.1
Msa0590320 AT3G26744.1 35.644 101 62 1 14 111 392 492 3.21e-11 58.9
Msa0590320 AT3G26744.4 35.644 101 62 1 14 111 392 492 3.21e-11 58.9
Msa0590320 AT3G26744.2 35.644 101 62 1 14 111 392 492 3.21e-11 58.9

Find 90 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACCAAAGCTAACTTATT+TGG 0.132124 4_2:-13729568 None:intergenic
TCTTCAATATCAATAATTAA+AGG 0.208328 4_2:+13729514 Msa0590320:three_prime_UTR
TGAATCACCTTGCTGATCTT+AGG 0.222682 4_2:-13727240 None:intergenic
AGGCTGTCCTTCAACCCTTT+TGG 0.223211 4_2:-13727142 None:intergenic
TTTAAAGCATGTGCATATTC+AGG 0.229476 4_2:-13729757 None:intergenic
AGTGATTGCTTTATCTCTTC+AGG 0.235636 4_2:-13729394 None:intergenic
TGGAATGAAGTTGAACAAAA+TGG 0.249862 4_2:-13726613 None:intergenic
CATAGATTTGAATGTCCTTA+TGG 0.286074 4_2:+13726898 Msa0590320:five_prime_UTR
TTATCAATGTACTACTAAAA+AGG 0.286143 4_2:-13729682 None:intergenic
ACAAATCAGATAAACCTTTC+AGG 0.295093 4_2:+13727625 Msa0590320:CDS
AAACCTTTCAGGAATTTCTA+AGG 0.299341 4_2:+13727636 Msa0590320:CDS
TTTACCACAACAAGAAAGTA+TGG 0.308343 4_2:+13726990 Msa0590320:five_prime_UTR
GTGAATGTCACAACTTTATA+AGG 0.308796 4_2:+13729848 Msa0590320:three_prime_UTR
TTCCCATGTGTTTAGGTAGA+TGG 0.318517 4_2:+13729352 Msa0590320:intron
TATCAATAATTAAAGGAATT+AGG 0.318963 4_2:+13729521 Msa0590320:three_prime_UTR
TCATCATACCCTTATCTTGA+TGG 0.320494 4_2:+13726931 Msa0590320:five_prime_UTR
CTTTCTTGTTGTGGTAAAAC+TGG 0.323789 4_2:-13726985 None:intergenic
AGATAAAGCAATCACTATTC+AGG 0.330105 4_2:+13729401 Msa0590320:CDS
TTTCTTACCATTACTTCATT+TGG 0.330225 4_2:-13727667 None:intergenic
CAAGAAAGTATGGAAGAGTT+TGG 0.335442 4_2:+13727000 Msa0590320:five_prime_UTR
GAGTCAAAGGTCCAACCATT+AGG 0.336189 4_2:-13726805 None:intergenic
ACAGCCTTCAAAGAATCTTA+TGG 0.358273 4_2:+13727158 Msa0590320:five_prime_UTR
ATGGAAGAGTTTGGATTTGT+TGG 0.361028 4_2:+13727009 Msa0590320:five_prime_UTR
CATTCAAATCTATGGTCTAA+TGG 0.372381 4_2:-13726889 None:intergenic
ACCCTATGTATGTTAAATAC+AGG 0.390422 4_2:-13729617 None:intergenic
CAGTAACAATCCTGGCTTTG+TGG 0.397442 4_2:-13727842 None:intergenic
GAATCACCTTGCTGATCTTA+GGG 0.398992 4_2:-13727239 None:intergenic
CATAAGGACATTCAAATCTA+TGG 0.410090 4_2:-13726897 None:intergenic
AACGAGTTACTACTCCCTAA+TGG 0.410425 4_2:+13726790 Msa0590320:five_prime_UTR
ATGGACAGGACATCAATTCT+AGG 0.415879 4_2:+13727529 Msa0590320:five_prime_UTR
ATCTGCTGTGCCACAAAGCC+AGG 0.421676 4_2:+13727832 Msa0590320:CDS
GCAAATGAAGGGTTTAAAGA+TGG 0.426120 4_2:-13726853 None:intergenic
CTATTCAGGAATGCAGGTTA+TGG 0.429722 4_2:+13729415 Msa0590320:CDS
GTGATTGCTTTATCTCTTCA+GGG 0.432133 4_2:-13729393 None:intergenic
TAGTAAGTTACAATAAGAAA+TGG 0.436128 4_2:+13727591 Msa0590320:exon
GAAGAAAGCGTTTGAATGAC+AGG 0.437266 4_2:+13727193 Msa0590320:five_prime_UTR
GATTCCAAATAAGTTAGCTT+TGG 0.441100 4_2:+13729564 Msa0590320:three_prime_UTR
GGAATGAAGTTGAACAAAAT+GGG 0.446885 4_2:-13726612 None:intergenic
TCATCCATAAGATTCTTTGA+AGG 0.448575 4_2:-13727162 None:intergenic
AGTCAAAGGTCCAACCATTA+GGG 0.456761 4_2:-13726804 None:intergenic
AAAATGGAACTTTCTCAACT+TGG 0.469304 4_2:+13726706 Msa0590320:five_prime_UTR
TATTCATAGTTCACAAAAGT+GGG 0.479190 4_2:+13726671 Msa0590320:five_prime_UTR
ATGACAGGCTTTCCATGCTT+AGG 0.479470 4_2:+13727208 Msa0590320:five_prime_UTR
GCCAAATCCAAAAGGGTTGA+AGG 0.482278 4_2:+13727135 Msa0590320:five_prime_UTR
CCCTGTATTTAACATACATA+GGG 0.490522 4_2:+13729616 Msa0590320:three_prime_UTR
ACTGAGAATCCATCAAGATA+AGG 0.498561 4_2:-13726940 None:intergenic
CTGAGAATCCATCAAGATAA+GGG 0.499837 4_2:-13726939 None:intergenic
ACATGAAAGAGCTTCTAGAA+AGG 0.506584 4_2:+13727566 Msa0590320:five_prime_UTR
AGATACTGATTCTTGTGTCC+TGG 0.512516 4_2:-13727813 None:intergenic
TTTAATTATTGATATTGAAG+AGG 0.512898 4_2:-13729512 None:intergenic
TCTTCCATACTTTCTTGTTG+TGG 0.521400 4_2:-13726994 None:intergenic
ACATGGAACACTTTATCCAC+TGG 0.522465 4_2:+13726763 Msa0590320:five_prime_UTR
CAATCACTATTCAGGAATGC+AGG 0.523750 4_2:+13729409 Msa0590320:CDS
AGATGTCTCTAGGAAAATGA+TGG 0.527614 4_2:+13729442 Msa0590320:exon
TAAGTTACAATAAGAAATGG+AGG 0.530314 4_2:+13727594 Msa0590320:exon
TTCAGGAATGCAGGTTATGG+TGG 0.533157 4_2:+13729418 Msa0590320:CDS
ATTAAAGCCAAATGAAGTAA+TGG 0.533491 4_2:+13727660 Msa0590320:CDS
TGATGTTGAGCGGAGAGACC+AGG 0.534371 4_2:+13727795 Msa0590320:CDS
AAAATCACTTTGAATCCACT+TGG 0.537566 4_2:-13729726 None:intergenic
AGAATCTTATGGATGAAAGA+AGG 0.538180 4_2:+13727169 Msa0590320:five_prime_UTR
ACCCTGTATTTAACATACAT+AGG 0.538761 4_2:+13729615 Msa0590320:three_prime_UTR
GATGATGCATCAGTTCCATA+AGG 0.540398 4_2:-13726913 None:intergenic
CCCTATGTATGTTAAATACA+GGG 0.541182 4_2:-13729616 None:intergenic
ATGTCACAACTTTATAAGGT+TGG 0.546930 4_2:+13729852 Msa0590320:three_prime_UTR
TTGTTGGAAGTGAGAACAAG+AGG 0.555475 4_2:+13727025 Msa0590320:five_prime_UTR
GCTGTTGACAGTAACAATCC+TGG 0.558322 4_2:-13727850 None:intergenic
TTATTCATAGTTCACAAAAG+TGG 0.563281 4_2:+13726670 Msa0590320:five_prime_UTR
TTATGGTGGGAGATGTCTCT+AGG 0.570179 4_2:+13729432 Msa0590320:CDS
AGTTACTACTCCCTAATGGT+TGG 0.572860 4_2:+13726794 Msa0590320:five_prime_UTR
ATTGCAAGCATCTTGCTCAA+AGG 0.575987 4_2:+13727939 Msa0590320:CDS
ATTCCTTAGAAATTCCTGAA+AGG 0.577724 4_2:-13727639 None:intergenic
GTTGAGAATGAAGCAAATGA+AGG 0.578645 4_2:-13726865 None:intergenic
GTTACAATAAGAAATGGAGG+AGG 0.581473 4_2:+13727597 Msa0590320:exon
TAAAATCAGCTGCAAAGTTG+AGG 0.582223 4_2:+13727059 Msa0590320:five_prime_UTR
GCGAACACATTAGAAGCATT+AGG 0.583837 4_2:+13727871 Msa0590320:CDS
GCCATTGCAACTTGATACAA+AGG 0.600246 4_2:-13729817 None:intergenic
TCCTTTGTATCAAGTTGCAA+TGG 0.600570 4_2:+13729816 Msa0590320:three_prime_UTR
TAACACACTGGTGAATCTCA+AGG 0.606173 4_2:-13727894 None:intergenic
TCAGGAATGCAGGTTATGGT+GGG 0.610095 4_2:+13729419 Msa0590320:CDS
TTGAGAATGAAGCAAATGAA+GGG 0.614806 4_2:-13726864 None:intergenic
TAGTAACTCGTTCAAACCAG+TGG 0.618072 4_2:-13726779 None:intergenic
AATGGTAAGAAATTCCCCTA+AGG 0.619045 4_2:+13727678 Msa0590320:CDS
CAATAAGAAATGGAGGAGGA+AGG 0.620297 4_2:+13727601 Msa0590320:exon
TGAAACTGCTTATAACACAC+TGG 0.622427 4_2:-13727906 None:intergenic
GGTGCTGAATCATCAAACTC+AGG 0.624297 4_2:-13726964 None:intergenic
AGGGACAATTGACCTAAGCA+TGG 0.627542 4_2:-13727220 None:intergenic
AAACAGAAAAGTCTTAACAA+TGG 0.636764 4_2:-13726633 None:intergenic
GGAACTAAACCATACCTTAG+GGG 0.677566 4_2:-13727692 None:intergenic
TAGTTCCAAGAAAAGACACA+TGG 0.690258 4_2:+13726746 Msa0590320:five_prime_UTR
AATTGTCCCTAAGATCAGCA+AGG 0.709185 4_2:+13727233 Msa0590320:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTGAATAAATTTAATTAA+TGG + chr4_2:13727747-13727766 Msa0590320:intron 10.0%
!!! TTTAATAAATTGAATTTTGA+TGG + chr4_2:13728397-13728416 Msa0590320:intron 10.0%
!!! TTAAAATTTTAAGGATTTTA+TGG - chr4_2:13728820-13728839 None:intergenic 10.0%
!!! CTATTGATTTTAAAATTTTA+AGG - chr4_2:13728829-13728848 None:intergenic 10.0%
!!! ATAGTAAAAGAATTTTTTAT+TGG + chr4_2:13728845-13728864 Msa0590320:intron 10.0%
!!! AAAATGTAAATTTTGTAAGA+TGG + chr4_2:13727510-13727529 Msa0590320:intron 15.0%
!! ATTAAATTTATTCAGAAACT+TGG - chr4_2:13727744-13727763 None:intergenic 15.0%
!!! GTTTTTTTTCTCTATAATTA+GGG + chr4_2:13728152-13728171 Msa0590320:intron 15.0%
!!! TTTTTTTTCTCTATAATTAG+GGG + chr4_2:13728153-13728172 Msa0590320:intron 15.0%
!!! TTTTTTTTGAAAGATTTGTA+AGG + chr4_2:13728671-13728690 Msa0590320:intron 15.0%
!!! TTTTTTTGAAAGATTTGTAA+GGG + chr4_2:13728672-13728691 Msa0590320:intron 15.0%
!! TAACAAATAAAAAATGTGTA+TGG + chr4_2:13728745-13728764 Msa0590320:intron 15.0%
!!! ATGGGATTTTAAAATTTTTA+AGG - chr4_2:13728970-13728989 None:intergenic 15.0%
!! TTTAATTATTGATATTGAAG+AGG - chr4_2:13729515-13729534 None:intergenic 15.0%
!! TCTTCAATATCAATAATTAA+AGG + chr4_2:13729514-13729533 Msa0590320:three_prime_UTR 15.0%
!! TATCAATAATTAAAGGAATT+AGG + chr4_2:13729521-13729540 Msa0590320:three_prime_UTR 15.0%
!!! ATGCTTTAAAATTTCTTTTT+TGG + chr4_2:13729770-13729789 Msa0590320:three_prime_UTR 15.0%
!!! TGCTTTAAAATTTCTTTTTT+GGG + chr4_2:13729771-13729790 Msa0590320:three_prime_UTR 15.0%
!!! ATGTATGTGTGTTTTATTTT+TGG + chr4_2:13727392-13727411 Msa0590320:intron 20.0%
!! TAGTAAGTTACAATAAGAAA+TGG + chr4_2:13727591-13727610 Msa0590320:exon 20.0%
!! ATAATTGATCATGAAAAGTT+TGG + chr4_2:13728067-13728086 Msa0590320:intron 20.0%
!!! CGTTTTTTTTCTCTATAATT+AGG + chr4_2:13728151-13728170 Msa0590320:intron 20.0%
!!! TTTTTTTCTCTATAATTAGG+GGG + chr4_2:13728154-13728173 Msa0590320:intron 20.0%
!! TTAATTATGAAAAAGTCTTG+TGG + chr4_2:13728217-13728236 Msa0590320:intron 20.0%
!! CAAAATCATTTAAATTTCGA+AGG + chr4_2:13728370-13728389 Msa0590320:intron 20.0%
!!! TTCAAAAAAAATTCTAAGAG+AGG - chr4_2:13728662-13728681 None:intergenic 20.0%
!!! ATATGTACAATTGAATTTTG+TGG + chr4_2:13728710-13728729 Msa0590320:intron 20.0%
!!! TATGTACAATTGAATTTTGT+GGG + chr4_2:13728711-13728730 Msa0590320:intron 20.0%
!!! TGGATTTTACAAGATTTTAA+GGG - chr4_2:13728800-13728819 None:intergenic 20.0%
!!! ATGGATTTTACAAGATTTTA+AGG - chr4_2:13728801-13728820 None:intergenic 20.0%
!!! TAAGGATTCAATGAATTTTA+GGG - chr4_2:13728952-13728971 None:intergenic 20.0%
!!! TTAAGGATTCAATGAATTTT+AGG - chr4_2:13728953-13728972 None:intergenic 20.0%
!!! ATTTATCACACTGAATTTTA+AGG - chr4_2:13729008-13729027 None:intergenic 20.0%
!! TTATCAATGTACTACTAAAA+AGG - chr4_2:13729685-13729704 None:intergenic 20.0%
!!! TTTTAGTAGTACATTGATAA+TGG + chr4_2:13729685-13729704 Msa0590320:three_prime_UTR 20.0%
! AAACAGAAAAGTCTTAACAA+TGG - chr4_2:13726636-13726655 None:intergenic 25.0%
!! ATTGTTAAGACTTTTCTGTT+TGG + chr4_2:13726635-13726654 Msa0590320:five_prime_UTR 25.0%
! TTATTCATAGTTCACAAAAG+TGG + chr4_2:13726670-13726689 Msa0590320:five_prime_UTR 25.0%
! TATTCATAGTTCACAAAAGT+GGG + chr4_2:13726671-13726690 Msa0590320:five_prime_UTR 25.0%
!! ATTTTCATCAAATGAGTCAA+AGG - chr4_2:13726821-13726840 None:intergenic 25.0%
! TAAGTTACAATAAGAAATGG+AGG + chr4_2:13727594-13727613 Msa0590320:exon 25.0%
! ATTAAAGCCAAATGAAGTAA+TGG + chr4_2:13727660-13727679 Msa0590320:CDS 25.0%
! TTTCTTACCATTACTTCATT+TGG - chr4_2:13727670-13727689 None:intergenic 25.0%
! GATTATGCATTAATTCTTCA+AGG - chr4_2:13727716-13727735 None:intergenic 25.0%
! AAATTGTATTACTGCATGTT+CGG + chr4_2:13727967-13727986 Msa0590320:intron 25.0%
!!! AAGCGTAGTTTTTAGTAAAA+TGG - chr4_2:13728031-13728050 None:intergenic 25.0%
!!! GTCATTTTAAAAAAGTCTTG+AGG + chr4_2:13728301-13728320 Msa0590320:intron 25.0%
! ATCACTTAAAAAAAGTCTCA+TGG + chr4_2:13728343-13728362 Msa0590320:intron 25.0%
!!! TTGAATTTTGATGGATTTTG+TGG + chr4_2:13728406-13728425 Msa0590320:intron 25.0%
!!! TGAATTTTGATGGATTTTGT+GGG + chr4_2:13728407-13728426 Msa0590320:intron 25.0%
! ATGAAAAGATGAGATTTCTT+GGG + chr4_2:13728466-13728485 Msa0590320:intron 25.0%
!! AAGGATTCAATGAATTTTAG+GGG - chr4_2:13728951-13728970 None:intergenic 25.0%
!! AGGATTTCAAAGGATTTTAT+GGG - chr4_2:13728988-13729007 None:intergenic 25.0%
!! AAGGATTTCAAAGGATTTTA+TGG - chr4_2:13728989-13729008 None:intergenic 25.0%
!!! CTGAATTTTAAGGATTTCAA+AGG - chr4_2:13728998-13729017 None:intergenic 25.0%
! AATCGAATGTAATAAATTCC+TGG - chr4_2:13729190-13729209 None:intergenic 25.0%
! TTTAACCATGCAAACAATTA+TGG - chr4_2:13729299-13729318 None:intergenic 25.0%
! TAAACCAAAGCTAACTTATT+TGG - chr4_2:13729571-13729590 None:intergenic 25.0%
GGAATGAAGTTGAACAAAAT+GGG - chr4_2:13726615-13726634 None:intergenic 30.0%
TGGAATGAAGTTGAACAAAA+TGG - chr4_2:13726616-13726635 None:intergenic 30.0%
TGGGAAAAGAGAAAGAAAAA+TGG + chr4_2:13726690-13726709 Msa0590320:five_prime_UTR 30.0%
AAAATGGAACTTTCTCAACT+TGG + chr4_2:13726706-13726725 Msa0590320:five_prime_UTR 30.0%
TTGAGAATGAAGCAAATGAA+GGG - chr4_2:13726867-13726886 None:intergenic 30.0%
CATTCAAATCTATGGTCTAA+TGG - chr4_2:13726892-13726911 None:intergenic 30.0%
CATAAGGACATTCAAATCTA+TGG - chr4_2:13726900-13726919 None:intergenic 30.0%
CATAGATTTGAATGTCCTTA+TGG + chr4_2:13726898-13726917 Msa0590320:five_prime_UTR 30.0%
TTTACCACAACAAGAAAGTA+TGG + chr4_2:13726990-13727009 Msa0590320:five_prime_UTR 30.0%
! TGAAACTCCAGTTTTTAACA+TGG + chr4_2:13727092-13727111 Msa0590320:five_prime_UTR 30.0%
! GAAACTCCAGTTTTTAACAT+GGG + chr4_2:13727093-13727112 Msa0590320:five_prime_UTR 30.0%
AGAAAAAAGCCAAATCCAAA+AGG + chr4_2:13727127-13727146 Msa0590320:five_prime_UTR 30.0%
GAAAAAAGCCAAATCCAAAA+GGG + chr4_2:13727128-13727147 Msa0590320:five_prime_UTR 30.0%
TCATCCATAAGATTCTTTGA+AGG - chr4_2:13727165-13727184 None:intergenic 30.0%
AGAATCTTATGGATGAAAGA+AGG + chr4_2:13727169-13727188 Msa0590320:five_prime_UTR 30.0%
! ATATCTTCAATCTGAAGTGT+CGG - chr4_2:13727318-13727337 None:intergenic 30.0%
TAGTCAATTGTAGTCAATGA+AGG - chr4_2:13727439-13727458 None:intergenic 30.0%
AATTGACTAGCACTTGTATT+TGG + chr4_2:13727450-13727469 Msa0590320:intron 30.0%
! GTAAATTTTGTAAGATGGAC+AGG + chr4_2:13727515-13727534 Msa0590320:intron 30.0%
ACAAATCAGATAAACCTTTC+AGG + chr4_2:13727625-13727644 Msa0590320:CDS 30.0%
AAACCTTTCAGGAATTTCTA+AGG + chr4_2:13727636-13727655 Msa0590320:CDS 30.0%
ATTCCTTAGAAATTCCTGAA+AGG - chr4_2:13727642-13727661 None:intergenic 30.0%
!! TTTTTCAGTTTGATGTTGAG+CGG + chr4_2:13727785-13727804 Msa0590320:intron 30.0%
!!! CGTAGTTTTTAGTAAAATGG+CGG - chr4_2:13728028-13728047 None:intergenic 30.0%
! TGTATTGGATTAGGATTCTA+TGG + chr4_2:13728178-13728197 Msa0590320:intron 30.0%
! GTATTGGATTAGGATTCTAT+GGG + chr4_2:13728179-13728198 Msa0590320:intron 30.0%
GCAACTTAAAAAAAGTCTTG+TGG + chr4_2:13728259-13728278 Msa0590320:intron 30.0%
CATGAAAAGATGAGATTTCT+TGG + chr4_2:13728465-13728484 Msa0590320:intron 30.0%
AATTCCTGGCGAAAAATTAA+GGG - chr4_2:13729176-13729195 None:intergenic 30.0%
AAATTCCTGGCGAAAAATTA+AGG - chr4_2:13729177-13729196 None:intergenic 30.0%
CATATTATTCCCATGTGTTT+AGG + chr4_2:13729345-13729364 Msa0590320:intron 30.0%
AGATAAAGCAATCACTATTC+AGG + chr4_2:13729401-13729420 Msa0590320:CDS 30.0%
ATTTGCAGATGATGAAAAAG+AGG + chr4_2:13729480-13729499 Msa0590320:three_prime_UTR 30.0%
! GATTCCAAATAAGTTAGCTT+TGG + chr4_2:13729564-13729583 Msa0590320:three_prime_UTR 30.0%
ACCCTGTATTTAACATACAT+AGG + chr4_2:13729615-13729634 Msa0590320:three_prime_UTR 30.0%
CCCTATGTATGTTAAATACA+GGG - chr4_2:13729619-13729638 None:intergenic 30.0%
CCCTGTATTTAACATACATA+GGG + chr4_2:13729616-13729635 Msa0590320:three_prime_UTR 30.0%
ACCCTATGTATGTTAAATAC+AGG - chr4_2:13729620-13729639 None:intergenic 30.0%
! AGGTGGAATTTTATCACAAT+GGG - chr4_2:13729665-13729684 None:intergenic 30.0%
! AAGGTGGAATTTTATCACAA+TGG - chr4_2:13729666-13729685 None:intergenic 30.0%
TCAATGTACTACTAAAAAGG+TGG - chr4_2:13729682-13729701 None:intergenic 30.0%
! TTTGAATCCACTTGGATTTT+TGG - chr4_2:13729721-13729740 None:intergenic 30.0%
AAAATCACTTTGAATCCACT+TGG - chr4_2:13729729-13729748 None:intergenic 30.0%
! TTTAAAGCATGTGCATATTC+AGG - chr4_2:13729760-13729779 None:intergenic 30.0%
GTGAATGTCACAACTTTATA+AGG + chr4_2:13729848-13729867 Msa0590320:three_prime_UTR 30.0%
!! TTCTCAACTTGGTTTTCTTG+AGG + chr4_2:13726717-13726736 Msa0590320:five_prime_UTR 35.0%
TAGTTCCAAGAAAAGACACA+TGG + chr4_2:13726746-13726765 Msa0590320:five_prime_UTR 35.0%
GCAAATGAAGGGTTTAAAGA+TGG - chr4_2:13726856-13726875 None:intergenic 35.0%
GTTGAGAATGAAGCAAATGA+AGG - chr4_2:13726868-13726887 None:intergenic 35.0%
TCATCATACCCTTATCTTGA+TGG + chr4_2:13726931-13726950 Msa0590320:five_prime_UTR 35.0%
CTGAGAATCCATCAAGATAA+GGG - chr4_2:13726942-13726961 None:intergenic 35.0%
ACTGAGAATCCATCAAGATA+AGG - chr4_2:13726943-13726962 None:intergenic 35.0%
! CTTTCTTGTTGTGGTAAAAC+TGG - chr4_2:13726988-13727007 None:intergenic 35.0%
TCTTCCATACTTTCTTGTTG+TGG - chr4_2:13726997-13727016 None:intergenic 35.0%
! CAAGAAAGTATGGAAGAGTT+TGG + chr4_2:13727000-13727019 Msa0590320:five_prime_UTR 35.0%
ATGGAAGAGTTTGGATTTGT+TGG + chr4_2:13727009-13727028 Msa0590320:five_prime_UTR 35.0%
TAAAATCAGCTGCAAAGTTG+AGG + chr4_2:13727059-13727078 Msa0590320:five_prime_UTR 35.0%
CACAAACCCATGTTAAAAAC+TGG - chr4_2:13727102-13727121 None:intergenic 35.0%
! GTTTTTAACATGGGTTTGTG+TGG + chr4_2:13727102-13727121 Msa0590320:five_prime_UTR 35.0%
ACAGCCTTCAAAGAATCTTA+TGG + chr4_2:13727158-13727177 Msa0590320:five_prime_UTR 35.0%
GTGTGTATGTTTATGTTGTC+TGG + chr4_2:13727290-13727309 Msa0590320:intron 35.0%
!!! CAGTTATTCTTTTTTGTGCC+TGG + chr4_2:13727341-13727360 Msa0590320:intron 35.0%
! ACATGAAAGAGCTTCTAGAA+AGG + chr4_2:13727566-13727585 Msa0590320:five_prime_UTR 35.0%
GTTACAATAAGAAATGGAGG+AGG + chr4_2:13727597-13727616 Msa0590320:exon 35.0%
AATGGTAAGAAATTCCCCTA+AGG + chr4_2:13727678-13727697 Msa0590320:CDS 35.0%
TAAGAAATTCCCCTAAGGTA+TGG + chr4_2:13727683-13727702 Msa0590320:intron 35.0%
AGGAACTAAACCATACCTTA+GGG - chr4_2:13727696-13727715 None:intergenic 35.0%
AAGGAACTAAACCATACCTT+AGG - chr4_2:13727697-13727716 None:intergenic 35.0%
TGAAACTGCTTATAACACAC+TGG - chr4_2:13727909-13727928 None:intergenic 35.0%
! AACTACGCTTTTGAGCTTTA+AGG + chr4_2:13728041-13728060 Msa0590320:intron 35.0%
!! ACTACGCTTTTGAGCTTTAA+GGG + chr4_2:13728042-13728061 Msa0590320:intron 35.0%
CTATAATTAGGGGGATGTAT+TGG + chr4_2:13728163-13728182 Msa0590320:intron 35.0%
! AAAAATTAAGGGGGGTGTAT+TGG - chr4_2:13729165-13729184 None:intergenic 35.0%
ATTCCTGGCGAAAAATTAAG+GGG - chr4_2:13729175-13729194 None:intergenic 35.0%
!!! CTAGTCTATTTTTGGTTCTG+CGG - chr4_2:13729270-13729289 None:intergenic 35.0%
AACCATGCAAACAATTATGG+AGG - chr4_2:13729296-13729315 None:intergenic 35.0%
GTGATTGCTTTATCTCTTCA+GGG - chr4_2:13729396-13729415 None:intergenic 35.0%
AGTGATTGCTTTATCTCTTC+AGG - chr4_2:13729397-13729416 None:intergenic 35.0%
! AGATGTCTCTAGGAAAATGA+TGG + chr4_2:13729442-13729461 Msa0590320:exon 35.0%
! TCCTTTGTATCAAGTTGCAA+TGG + chr4_2:13729816-13729835 Msa0590320:three_prime_UTR 35.0%
! GTGTTCCATGTGTCTTTTCT+TGG - chr4_2:13726754-13726773 None:intergenic 40.0%
ACATGGAACACTTTATCCAC+TGG + chr4_2:13726763-13726782 Msa0590320:five_prime_UTR 40.0%
TAGTAACTCGTTCAAACCAG+TGG - chr4_2:13726782-13726801 None:intergenic 40.0%
AACGAGTTACTACTCCCTAA+TGG + chr4_2:13726790-13726809 Msa0590320:five_prime_UTR 40.0%
AGTTACTACTCCCTAATGGT+TGG + chr4_2:13726794-13726813 Msa0590320:five_prime_UTR 40.0%
AGTCAAAGGTCCAACCATTA+GGG - chr4_2:13726807-13726826 None:intergenic 40.0%
GATGATGCATCAGTTCCATA+AGG - chr4_2:13726916-13726935 None:intergenic 40.0%
TTGTTGGAAGTGAGAACAAG+AGG + chr4_2:13727025-13727044 Msa0590320:five_prime_UTR 40.0%
! TCCTTCAACCCTTTTGGATT+TGG - chr4_2:13727139-13727158 None:intergenic 40.0%
!! GAAGAAAGCGTTTGAATGAC+AGG + chr4_2:13727193-13727212 Msa0590320:five_prime_UTR 40.0%
AATTGTCCCTAAGATCAGCA+AGG + chr4_2:13727233-13727252 Msa0590320:five_prime_UTR 40.0%
GAATCACCTTGCTGATCTTA+GGG - chr4_2:13727242-13727261 None:intergenic 40.0%
TGAATCACCTTGCTGATCTT+AGG - chr4_2:13727243-13727262 None:intergenic 40.0%
TGAGCACTTACATAAACACC+AGG - chr4_2:13727362-13727381 None:intergenic 40.0%
ATGGACAGGACATCAATTCT+AGG + chr4_2:13727529-13727548 Msa0590320:five_prime_UTR 40.0%
CAATAAGAAATGGAGGAGGA+AGG + chr4_2:13727601-13727620 Msa0590320:exon 40.0%
GGAACTAAACCATACCTTAG+GGG - chr4_2:13727695-13727714 None:intergenic 40.0%
!! AGATACTGATTCTTGTGTCC+TGG - chr4_2:13727816-13727835 None:intergenic 40.0%
!! GCGAACACATTAGAAGCATT+AGG + chr4_2:13727871-13727890 Msa0590320:CDS 40.0%
TAACACACTGGTGAATCTCA+AGG - chr4_2:13727897-13727916 None:intergenic 40.0%
ATTGCAAGCATCTTGCTCAA+AGG + chr4_2:13727939-13727958 Msa0590320:CDS 40.0%
TTAGGGGGATGTATTGGATT+AGG + chr4_2:13728169-13728188 Msa0590320:intron 40.0%
ATAGAGAGACGAAGAGAGAA+AGG - chr4_2:13728558-13728577 None:intergenic 40.0%
AGAGAGAGAGAGAGAGAAAA+TGG - chr4_2:13728585-13728604 None:intergenic 40.0%
TTCCTGGCGAAAAATTAAGG+GGG - chr4_2:13729174-13729193 None:intergenic 40.0%
!! GGAGGCAACTAGTCTATTTT+TGG - chr4_2:13729278-13729297 None:intergenic 40.0%
TGCCTCCATAATTGTTTGCA+TGG + chr4_2:13729291-13729310 Msa0590320:intron 40.0%
TTCCCATGTGTTTAGGTAGA+TGG + chr4_2:13729352-13729371 Msa0590320:intron 40.0%
GACCATCTACCTAAACACAT+GGG - chr4_2:13729357-13729376 None:intergenic 40.0%
TGACCATCTACCTAAACACA+TGG - chr4_2:13729358-13729377 None:intergenic 40.0%
CAATCACTATTCAGGAATGC+AGG + chr4_2:13729409-13729428 Msa0590320:CDS 40.0%
CTATTCAGGAATGCAGGTTA+TGG + chr4_2:13729415-13729434 Msa0590320:CDS 40.0%
GTTGTTGCCAAAAATCCAAG+TGG + chr4_2:13729711-13729730 Msa0590320:three_prime_UTR 40.0%
GCCATTGCAACTTGATACAA+AGG - chr4_2:13729820-13729839 None:intergenic 40.0%
GAGTCAAAGGTCCAACCATT+AGG - chr4_2:13726808-13726827 None:intergenic 45.0%
! GGTGCTGAATCATCAAACTC+AGG - chr4_2:13726967-13726986 None:intergenic 45.0%
GCCAAATCCAAAAGGGTTGA+AGG + chr4_2:13727135-13727154 Msa0590320:five_prime_UTR 45.0%
ATGACAGGCTTTCCATGCTT+AGG + chr4_2:13727208-13727227 Msa0590320:five_prime_UTR 45.0%
AGGGACAATTGACCTAAGCA+TGG - chr4_2:13727223-13727242 None:intergenic 45.0%
!! CAGTAACAATCCTGGCTTTG+TGG - chr4_2:13727845-13727864 None:intergenic 45.0%
GCTGTTGACAGTAACAATCC+TGG - chr4_2:13727853-13727872 None:intergenic 45.0%
ACGAAGAGAGAAAGGAGAGA+GGG - chr4_2:13728550-13728569 None:intergenic 45.0%
! TAAGGGGGGTGTATTGGATT+AGG - chr4_2:13729159-13729178 None:intergenic 45.0%
TCCTGGCGAAAAATTAAGGG+GGG - chr4_2:13729173-13729192 None:intergenic 45.0%
TTCAGGAATGCAGGTTATGG+TGG + chr4_2:13729418-13729437 Msa0590320:CDS 45.0%
TCAGGAATGCAGGTTATGGT+GGG + chr4_2:13729419-13729438 Msa0590320:CDS 45.0%
! TTATGGTGGGAGATGTCTCT+AGG + chr4_2:13729432-13729451 Msa0590320:CDS 45.0%
! AGGCTGTCCTTCAACCCTTT+TGG - chr4_2:13727145-13727164 None:intergenic 50.0%
AAGAGAGAAAGGAGAGAGGG+GGG - chr4_2:13728547-13728566 None:intergenic 50.0%
GAAGAGAGAAAGGAGAGAGG+GGG - chr4_2:13728548-13728567 None:intergenic 50.0%
CGAAGAGAGAAAGGAGAGAG+GGG - chr4_2:13728549-13728568 None:intergenic 50.0%
GACGAAGAGAGAAAGGAGAG+AGG - chr4_2:13728551-13728570 None:intergenic 50.0%
GAGAGAGAGAGAAGAGAGGA+GGG - chr4_2:13728638-13728657 None:intergenic 50.0%
TGAGAGAGAGAGAAGAGAGG+AGG - chr4_2:13728639-13728658 None:intergenic 50.0%
AGGTGAGAGAGAGAGAAGAG+AGG - chr4_2:13728642-13728661 None:intergenic 50.0%
! ACCCCCCTTAATTTTTCGCC+AGG + chr4_2:13729169-13729188 Msa0590320:intron 50.0%
TGATGTTGAGCGGAGAGACC+AGG + chr4_2:13727795-13727814 Msa0590320:CDS 55.0%
ATCTGCTGTGCCACAAAGCC+AGG + chr4_2:13727832-13727851 Msa0590320:CDS 55.0%
GAGAGAAGAGAGGAGGGAGA+GGG - chr4_2:13728632-13728651 None:intergenic 55.0%
AGAGAGAAGAGAGGAGGGAG+AGG - chr4_2:13728633-13728652 None:intergenic 55.0%
AGAAAGGAGAGAGGGGGGAG+GGG - chr4_2:13728542-13728561 None:intergenic 60.0%
GAGAAAGGAGAGAGGGGGGA+GGG - chr4_2:13728543-13728562 None:intergenic 60.0%
AGAGAAAGGAGAGAGGGGGG+AGG - chr4_2:13728544-13728563 None:intergenic 60.0%
GAAGAGAGGAGGGAGAGGGA+GGG - chr4_2:13728628-13728647 None:intergenic 60.0%
AGAAGAGAGGAGGGAGAGGG+AGG - chr4_2:13728629-13728648 None:intergenic 60.0%
GGGGGAGGGGAGAGAGAGAA+GGG - chr4_2:13728529-13728548 None:intergenic 65.0%
AGAGGAGGGAGAGGGAGGGA+GGG - chr4_2:13728624-13728643 None:intergenic 65.0%
GGGGGGAGGGGAGAGAGAGA+AGG - chr4_2:13728530-13728549 None:intergenic 70.0%
GAGAGGAGGGAGAGGGAGGG+AGG - chr4_2:13728625-13728644 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 13726559 13729874 13726559 ID=Msa0590320;Name=Msa0590320
chr4_2 mRNA 13726559 13729874 13726559 ID=Msa0590320-mRNA-1;Parent=Msa0590320;Name=Msa0590320-mRNA-1;_AED=0.06;_eAED=0.06;_QI=777|1|1|1|0.66|0.75|4|421|114
chr4_2 exon 13726559 13727254 13726559 ID=Msa0590320-mRNA-1:exon:4019;Parent=Msa0590320-mRNA-1
chr4_2 exon 13727529 13727699 13727529 ID=Msa0590320-mRNA-1:exon:4020;Parent=Msa0590320-mRNA-1
chr4_2 exon 13727793 13727960 13727793 ID=Msa0590320-mRNA-1:exon:4021;Parent=Msa0590320-mRNA-1
chr4_2 exon 13729367 13729874 13729367 ID=Msa0590320-mRNA-1:exon:4022;Parent=Msa0590320-mRNA-1
chr4_2 five_prime_UTR 13726559 13727254 13726559 ID=Msa0590320-mRNA-1:five_prime_utr;Parent=Msa0590320-mRNA-1
chr4_2 five_prime_UTR 13727529 13727609 13727529 ID=Msa0590320-mRNA-1:five_prime_utr;Parent=Msa0590320-mRNA-1
chr4_2 CDS 13727610 13727699 13727610 ID=Msa0590320-mRNA-1:cds;Parent=Msa0590320-mRNA-1
chr4_2 CDS 13727793 13727960 13727793 ID=Msa0590320-mRNA-1:cds;Parent=Msa0590320-mRNA-1
chr4_2 CDS 13729367 13729453 13729367 ID=Msa0590320-mRNA-1:cds;Parent=Msa0590320-mRNA-1
chr4_2 three_prime_UTR 13729454 13729874 13729454 ID=Msa0590320-mRNA-1:three_prime_utr;Parent=Msa0590320-mRNA-1
Gene Sequence

>Msa0590320

ATGGAGGAGGAAGGAACAAATCAGATAAACCTTTCAGGAATTTCTAAGGAATTAAAGCCAAATGAAGTAATGGTAAGAAATTCCCCTAAGTTTGATGTTGAGCGGAGAGACCAGGACACAAGAATCAGTATCTGCTGTGCCACAAAGCCAGGATTGTTACTGTCAACAGCGAACACATTAGAAGCATTAGGCCTTGAGATTCACCAGTGTGTTATAAGCAGTTTCAATGATTTTTCATTGCAAGCATCTTGCTCAAAGGTAGATGGTCAGAGAAATTGTATGAACCCTGAAGAGATAAAGCAATCACTATTCAGGAATGCAGGTTATGGTGGGAGATGTCTCTAG
Protein sequence

>Msa0590320

MEEEGTNQINLSGISKELKPNEVMVRNSPKFDVERRDQDTRISICCATKPGLLLSTANTLEALGLEIHQCVISSFNDFSLQASCSKVDGQRNCMNPEEIKQSLFRNAGYGGRCL*