AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0626440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626440 MtrunA17_Chr8g0342651 63.415 123 13 1 103 225 88 178 4.94e-41 140
Msa0626440 MtrunA17_Chr8g0342651 49.351 77 31 2 64 140 149 217 4.30e-11 61.2
Msa0626440 MtrunA17_Chr1g0212341 76.471 68 10 2 158 219 21 88 1.74e-23 94.4
Msa0626440 MtrunA17_Chr1g0212341 41.885 191 66 5 64 254 65 210 1.48e-15 73.2
Msa0626440 MtrunA17_Chr5g0438531 55.932 59 22 1 104 158 69 127 4.67e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCATTACTTACATTATTT+TGG 0.125240 4_3:+6020521 Msa0626440:three_prime_UTR
ACGATGATTACCTTTGATTT+TGG 0.169382 4_3:-6019645 None:intergenic
TGTCATCCTTCAATTATATT+TGG 0.184257 4_3:+6018182 Msa0626440:five_prime_UTR
TTGGATTGTTATGTGATATT+AGG 0.202665 4_3:-6018101 None:intergenic
AGATCTTCGCGAAGCTAAAT+TGG 0.247966 4_3:+6018737 Msa0626440:CDS
CTATTGAACCGGCTTGATTT+AGG 0.266233 4_3:+6020185 Msa0626440:three_prime_UTR
GAACACAAGCAGTTCATAAT+TGG 0.276454 4_3:-6020811 None:intergenic
CTAGTTGAATCAAAGCTTCT+TGG 0.282657 4_3:-6019259 None:intergenic
GAGGAGTAGTGGTAGCATTT+TGG 0.290072 4_3:+6020141 Msa0626440:exon
GTCATCCTTCAATTATATTT+GGG 0.305842 4_3:+6018183 Msa0626440:five_prime_UTR
AACTTGCCAAATGCATTATC+AGG 0.326793 4_3:+6020363 Msa0626440:three_prime_UTR
TCGCTTTCTAAATTTATATT+CGG 0.331894 4_3:+6018644 Msa0626440:CDS
GTAAAGTAACATTGCATAAT+GGG 0.344030 4_3:+6020780 Msa0626440:three_prime_UTR
AGTAAAGTAACATTGCATAA+TGG 0.344143 4_3:+6020779 Msa0626440:three_prime_UTR
TGTTAATTGTTTAAAATTGA+AGG 0.345557 4_3:-6018149 None:intergenic
TATGTAACGAAATAATAAAT+TGG 0.353966 4_3:-6018120 None:intergenic
TAACTGAGGGATCTGTTAGA+TGG 0.365103 4_3:+6020443 Msa0626440:three_prime_UTR
TCCTACTTAACCAGATGCTT+TGG 0.368865 4_3:+6020499 Msa0626440:three_prime_UTR
CCGAAGGATCATCCATTATA+CGG 0.375242 4_3:+6018618 Msa0626440:CDS
AGGAGAAGAACGAACCATTT+GGG 0.385062 4_3:-6019302 None:intergenic
CTGAAACCTGATAATGCATT+TGG 0.390036 4_3:-6020369 None:intergenic
TGCGTGTCAACGGGTGCAAC+AGG 0.392472 4_3:-6019605 None:intergenic
AATCAAAGCTTCTTGGGTAA+TGG 0.393707 4_3:-6019252 None:intergenic
TCTTCCACCTCTCCTGGTGC+AGG 0.394878 4_3:-6020108 None:intergenic
ATTCGCTGCGCTCACTAGTT+CGG 0.405936 4_3:+6020676 Msa0626440:three_prime_UTR
AGAAAGCGACAACCGTATAA+TGG 0.406074 4_3:-6018630 None:intergenic
ACTTGATTTCTCTATTGAAC+CGG 0.410703 4_3:+6020174 Msa0626440:three_prime_UTR
GTTATATAAGTACGAAGATA+TGG 0.417339 4_3:+6018683 Msa0626440:CDS
TTCGACCCAAATATAATTGA+AGG 0.423577 4_3:-6018188 None:intergenic
GGGATATGATAAGGAAGATA+AGG 0.437427 4_3:+6020059 Msa0626440:CDS
CTACAATTGTGCGTGTCAAC+GGG 0.442078 4_3:-6019614 None:intergenic
CTTCCACCTCTCCTGGTGCA+GGG 0.450689 4_3:-6020107 None:intergenic
GTTAACTCTTCCACCTCTCC+TGG 0.451248 4_3:-6020114 None:intergenic
AGACTAAAATTTCACCTCTC+AGG 0.451846 4_3:-6018816 None:intergenic
ATATTACAAAATAGACCATC+AGG 0.453264 4_3:-6020655 None:intergenic
CCTTGCCGCCACCATGGACT+TGG 0.453782 4_3:+6018590 Msa0626440:exon
ATACACACACACAAGTGCTT+TGG 0.457560 4_3:+6018245 Msa0626440:five_prime_UTR
CCGTATAATGGATGATCCTT+CGG 0.457729 4_3:-6018618 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4_3:+6018928 Msa0626440:CDS
TAGTTGAATCAAAGCTTCTT+GGG 0.458718 4_3:-6019258 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4_3:+6019921 Msa0626440:CDS
TCACAAATCTTCTTTGCTGC+AGG 0.460278 4_3:-6019173 None:intergenic
CAAGTCCATGGTGGCGGCAA+GGG 0.461283 4_3:-6018589 None:intergenic
GGATCAGGAGGGATATGATA+AGG 0.465606 4_3:+6020050 Msa0626440:intron
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4_3:+6018927 Msa0626440:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4_3:+6019920 Msa0626440:CDS
GGAAGGCCAATCTGATGATA+AGG 0.467371 4_3:+6020080 Msa0626440:CDS
ATATTCGGACGATGCACTCT+TGG 0.468746 4_3:+6018659 Msa0626440:CDS
AAGTAATGAACCAAAGCATC+TGG 0.474408 4_3:-6020509 None:intergenic
GCTACAATTGTGCGTGTCAA+CGG 0.476888 4_3:-6019615 None:intergenic
AGCCCCTGCACCAGGAGAGG+TGG 0.481468 4_3:+6020104 Msa0626440:CDS
GGGCTTCCTTATCATCAGAT+TGG 0.481597 4_3:-6020086 None:intergenic
TTCCACCTCTCCTGGTGCAG+GGG 0.483421 4_3:-6020106 None:intergenic
ATCATCCACAATATTCAGCC+AGG 0.493124 4_3:+6018283 Msa0626440:five_prime_UTR
GTTGTAGAAAATAATGTAGC+AGG 0.498115 4_3:-6020314 None:intergenic
AGTTAACAGATGAGGAGTAG+TGG 0.515594 4_3:+6020130 Msa0626440:CDS
GATAAGGAAGCCCCTGCACC+AGG 0.518868 4_3:+6020096 Msa0626440:CDS
CTGCGAAAAGGGCTAAAGTT+GGG 0.522171 4_3:+6018939 Msa0626440:CDS
ACGATTGATCCTATCCCTGA+TGG 0.525163 4_3:+6020640 Msa0626440:three_prime_UTR
GGCAAGTTTAACATGATCGC+AGG 0.528195 4_3:-6020348 None:intergenic
GTGACAAAGATGGCCCCAAA+TGG 0.530435 4_3:+6019288 Msa0626440:CDS
CAATGAAAGTACCGTAGCCC+TGG 0.537406 4_3:-6018301 None:intergenic
ACCAAAGCATCTGGTTAAGT+AGG 0.541232 4_3:-6020500 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.541934 4_3:+6018938 Msa0626440:CDS
CCAAGTCCATGGTGGCGGCA+AGG 0.543580 4_3:-6018590 None:intergenic
AACTGAGGGATCTGTTAGAT+GGG 0.547128 4_3:+6020444 Msa0626440:three_prime_UTR
CAAAATAGACCATCAGGGAT+AGG 0.548322 4_3:-6020649 None:intergenic
CAACTCCAAGTCCATGGTGG+CGG 0.554736 4_3:-6018595 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.558971 4_3:+6019931 Msa0626440:CDS
ACAATATTCAGCCAGGGCTA+CGG 0.567396 4_3:+6018290 Msa0626440:five_prime_UTR
TGGGCAATGACTTCTGTGAA+TGG 0.568132 4_3:+6020463 Msa0626440:three_prime_UTR
GGAAGCCCCTGCACCAGGAG+AGG 0.576902 4_3:+6020101 Msa0626440:CDS
GGTGGAAGAGTTAACAGATG+AGG 0.581307 4_3:+6020122 Msa0626440:CDS
AAAAGGGCTAAAGTTGGGAA+AGG 0.581404 4_3:+6018944 Msa0626440:CDS
AGAAACCCTTGCCGCCACCA+TGG 0.582020 4_3:+6018584 Msa0626440:intron
CTTCGGCAACTCCAAGTCCA+TGG 0.589099 4_3:-6018601 None:intergenic
TATTTATGTTTAGGATCAGG+AGG 0.589442 4_3:+6020038 Msa0626440:intron
AGCTTTAGCCTAAATCAAGC+CGG 0.591356 4_3:-6020193 None:intergenic
GTAGCCCTGGCTGAATATTG+TGG 0.592581 4_3:-6018288 None:intergenic
AGCCTTGAAGCCAAAATCAA+AGG 0.601595 4_3:+6019635 Msa0626440:CDS
CATGGACTTGGAGTTGCCGA+AGG 0.601960 4_3:+6018602 Msa0626440:exon
TTTAAAATCGAAGCAGCAGG+AGG 0.604752 4_3:+6019109 Msa0626440:intron
ATTTATGTTTAGGATCAGGA+GGG 0.610871 4_3:+6020039 Msa0626440:intron
CTAGAAATAAGTGACAAAGA+TGG 0.611157 4_3:+6019278 Msa0626440:CDS
AAAGGGCTAAAGTTGGGAAA+GGG 0.618574 4_3:+6018945 Msa0626440:CDS
TATGATAAGGAAGATAAGGA+AGG 0.621270 4_3:+6020063 Msa0626440:CDS
GGAGAAGAACGAACCATTTG+GGG 0.645082 4_3:-6019301 None:intergenic
TCATCCACAATATTCAGCCA+GGG 0.650109 4_3:+6018284 Msa0626440:five_prime_UTR
CAGGCATTAGCAATATTCCA+AGG 0.651084 4_3:-6020295 None:intergenic
ATGATCGCAGGATATAGAAG+AGG 0.655857 4_3:-6020336 None:intergenic
CTGAAGTGAAGAAACCTGAG+AGG 0.670696 4_3:+6018802 Msa0626440:CDS
CGGCAACTCCAAGTCCATGG+TGG 0.682013 4_3:-6018598 None:intergenic
TATTACAAAATAGACCATCA+GGG 0.682398 4_3:-6020654 None:intergenic
ATATAAGTACGAAGATATGG+TGG 0.694754 4_3:+6018686 Msa0626440:CDS
CTGAAGTGAAGAAACCCGAG+AGG 0.719762 4_3:+6019788 Msa0626440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTGTTAGTATTT+AGG + chr4_3:6018860-6018879 Msa0626440:intron 10.0%
!!! AGGTTTATTTATTTTTTTTT+TGG + chr4_3:6018880-6018899 Msa0626440:intron 10.0%
!!! GTATTTGATTTTATTTAATT+TGG + chr4_3:6019733-6019752 Msa0626440:intron 10.0%
!! TATGTAACGAAATAATAAAT+TGG - chr4_3:6018123-6018142 None:intergenic 15.0%
!!! TGTTAATTGTTTAAAATTGA+AGG - chr4_3:6018152-6018171 None:intergenic 15.0%
!!! TTTTTTTTTTGGAATTTTGT+AGG + chr4_3:6018891-6018910 Msa0626440:intron 15.0%
!!! AAGTATGTTTTCTTTTAATT+TGG + chr4_3:6018999-6019018 Msa0626440:intron 15.0%
!!! AGTATGTTTTCTTTTAATTT+GGG + chr4_3:6019000-6019019 Msa0626440:intron 15.0%
!!! TTGATAATAATTCCTAATTA+GGG - chr4_3:6019684-6019703 None:intergenic 15.0%
!!! TTTGATAATAATTCCTAATT+AGG - chr4_3:6019685-6019704 None:intergenic 15.0%
!! AATATTCACAAACTAAAATT+GGG - chr4_3:6019826-6019845 None:intergenic 15.0%
!!! TTTAGGTTTATCTATTTTTT+TGG + chr4_3:6019873-6019892 Msa0626440:intron 15.0%
!!! TTATAACTTTACTTTTGTAT+CGG + chr4_3:6020551-6020570 Msa0626440:three_prime_UTR 15.0%
!!! ATTTTGAAGTGTTATTAACA+AGG - chr4_3:6018221-6018240 None:intergenic 20.0%
!! AATCAATTTATGTCGAAAAA+CGG + chr4_3:6018439-6018458 Msa0626440:intron 20.0%
!! TCGCTTTCTAAATTTATATT+CGG + chr4_3:6018644-6018663 Msa0626440:CDS 20.0%
!! TTCAATGTAATTATAAGTGT+CGG + chr4_3:6019401-6019420 Msa0626440:intron 20.0%
!! ATATTCACAAACTAAAATTG+GGG - chr4_3:6019825-6019844 None:intergenic 20.0%
!! CAATATTCACAAACTAAAAT+TGG - chr4_3:6019827-6019846 None:intergenic 20.0%
!!! CAATTTTAGTTTGTGAATAT+TGG + chr4_3:6019825-6019844 Msa0626440:intron 20.0%
!!! CTATTTTTTTGGAATTTTGT+AGG + chr4_3:6019884-6019903 Msa0626440:intron 20.0%
!!! GTTCATTACTTACATTATTT+TGG + chr4_3:6020521-6020540 Msa0626440:three_prime_UTR 20.0%
!!! TACTTACATTATTTTGGATT+AGG + chr4_3:6020527-6020546 Msa0626440:three_prime_UTR 20.0%
!!! TATAACTTTACTTTTGTATC+GGG + chr4_3:6020552-6020571 Msa0626440:three_prime_UTR 20.0%
! TTGGATTGTTATGTGATATT+AGG - chr4_3:6018104-6018123 None:intergenic 25.0%
! TGTCATCCTTCAATTATATT+TGG + chr4_3:6018182-6018201 Msa0626440:five_prime_UTR 25.0%
! GTCATCCTTCAATTATATTT+GGG + chr4_3:6018183-6018202 Msa0626440:five_prime_UTR 25.0%
! TTCATATTTCGAAAAACCTA+AGG + chr4_3:6018407-6018426 Msa0626440:intron 25.0%
! AAAACGGTTATTCTTTGTAT+TGG + chr4_3:6018455-6018474 Msa0626440:intron 25.0%
! GTTATATAAGTACGAAGATA+TGG + chr4_3:6018683-6018702 Msa0626440:CDS 25.0%
!! TTTTAGTCTGTGAATATTGT+TGG + chr4_3:6018830-6018849 Msa0626440:intron 25.0%
! GAATCATTTCTTCAAAAACA+TGG - chr4_3:6019148-6019167 None:intergenic 25.0%
! AAAAATTCTCACAAAAGTCT+TGG + chr4_3:6019214-6019233 Msa0626440:CDS 25.0%
! TTTCAAATTATTAGCTGTGT+CGG - chr4_3:6019369-6019388 None:intergenic 25.0%
! TCAATGTAATTATAAGTGTC+GGG + chr4_3:6019402-6019421 Msa0626440:intron 25.0%
! ATTACTCATAAACCCTAATT+AGG + chr4_3:6019669-6019688 Msa0626440:intron 25.0%
!! TCTTGTTTGTGTTAGTATTT+AGG + chr4_3:6019850-6019869 Msa0626440:intron 25.0%
!! TTGTGTTAGTATTTAGGTTT+AGG + chr4_3:6019856-6019875 Msa0626440:intron 25.0%
!! CTATATTTATGTTTAGGATC+AGG + chr4_3:6020035-6020054 Msa0626440:intron 25.0%
!!! ATAACTTTACTTTTGTATCG+GGG + chr4_3:6020553-6020572 Msa0626440:three_prime_UTR 25.0%
! TATTACAAAATAGACCATCA+GGG - chr4_3:6020657-6020676 None:intergenic 25.0%
! ATATTACAAAATAGACCATC+AGG - chr4_3:6020658-6020677 None:intergenic 25.0%
! AGTAAAGTAACATTGCATAA+TGG + chr4_3:6020779-6020798 Msa0626440:three_prime_UTR 25.0%
! GTAAAGTAACATTGCATAAT+GGG + chr4_3:6020780-6020799 Msa0626440:three_prime_UTR 25.0%
TTCGACCCAAATATAATTGA+AGG - chr4_3:6018191-6018210 None:intergenic 30.0%
TTACCTATTTCATTCTGCAT+CGG + chr4_3:6018378-6018397 Msa0626440:intron 30.0%
!! ATTGATTCGTTTACAACCTT+AGG - chr4_3:6018426-6018445 None:intergenic 30.0%
ATATAAGTACGAAGATATGG+TGG + chr4_3:6018686-6018705 Msa0626440:CDS 30.0%
TAGTTGAATCAAAGCTTCTT+GGG - chr4_3:6019261-6019280 None:intergenic 30.0%
CTAGAAATAAGTGACAAAGA+TGG + chr4_3:6019278-6019297 Msa0626440:CDS 30.0%
! CAATGTAATTATAAGTGTCG+GGG + chr4_3:6019403-6019422 Msa0626440:intron 30.0%
!! ACGATGATTACCTTTGATTT+TGG - chr4_3:6019648-6019667 None:intergenic 30.0%
! AGTTGGAGTATGTTTTCTTT+TGG + chr4_3:6019948-6019967 Msa0626440:intron 30.0%
! GTTGGAGTATGTTTTCTTTT+GGG + chr4_3:6019949-6019968 Msa0626440:intron 30.0%
!! CTGGTGCTATATTTATGTTT+AGG + chr4_3:6020029-6020048 Msa0626440:intron 30.0%
! TATTTATGTTTAGGATCAGG+AGG + chr4_3:6020038-6020057 Msa0626440:intron 30.0%
! ATTTATGTTTAGGATCAGGA+GGG + chr4_3:6020039-6020058 Msa0626440:intron 30.0%
TATGATAAGGAAGATAAGGA+AGG + chr4_3:6020063-6020082 Msa0626440:CDS 30.0%
ACTTGATTTCTCTATTGAAC+CGG + chr4_3:6020174-6020193 Msa0626440:three_prime_UTR 30.0%
GTTGTAGAAAATAATGTAGC+AGG - chr4_3:6020317-6020336 None:intergenic 30.0%
! TTCTTTTCTGCATGTTTACT+AGG + chr4_3:6020409-6020428 Msa0626440:three_prime_UTR 30.0%
! TCTTTTCTGCATGTTTACTA+GGG + chr4_3:6020410-6020429 Msa0626440:three_prime_UTR 30.0%
AGACTAAAATTTCACCTCTC+AGG - chr4_3:6018819-6018838 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - chr4_3:6018923-6018942 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + chr4_3:6018928-6018947 Msa0626440:CDS 35.0%
! TTGTTTAAAATCGAAGCAGC+AGG + chr4_3:6019106-6019125 Msa0626440:intron 35.0%
AATCAAAGCTTCTTGGGTAA+TGG - chr4_3:6019255-6019274 None:intergenic 35.0%
CTAGTTGAATCAAAGCTTCT+TGG - chr4_3:6019262-6019281 None:intergenic 35.0%
!!! TACCTTTGATTTTGGCTTCA+AGG - chr4_3:6019640-6019659 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - chr4_3:6018923-6018942 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + chr4_3:6018928-6018947 Msa0626440:CDS 35.0%
GGGATATGATAAGGAAGATA+AGG + chr4_3:6020059-6020078 Msa0626440:CDS 35.0%
AACTTGCCAAATGCATTATC+AGG + chr4_3:6020363-6020382 Msa0626440:three_prime_UTR 35.0%
! CTGAAACCTGATAATGCATT+TGG - chr4_3:6020372-6020391 None:intergenic 35.0%
! CTTTTCTGCATGTTTACTAG+GGG + chr4_3:6020411-6020430 Msa0626440:three_prime_UTR 35.0%
! AGGGGAAATTTTCTTAACTG+AGG + chr4_3:6020429-6020448 Msa0626440:three_prime_UTR 35.0%
! GGGGAAATTTTCTTAACTGA+GGG + chr4_3:6020430-6020449 Msa0626440:three_prime_UTR 35.0%
! TGGTTAAGTAGGATTTTCTC+TGG - chr4_3:6020492-6020511 None:intergenic 35.0%
! AAGTAATGAACCAAAGCATC+TGG - chr4_3:6020512-6020531 None:intergenic 35.0%
!!! GGTATTTTAGCTCACTGTTT+TGG + chr4_3:6020697-6020716 Msa0626440:three_prime_UTR 35.0%
GAACACAAGCAGTTCATAAT+TGG - chr4_3:6020814-6020833 None:intergenic 35.0%
ATACACACACACAAGTGCTT+TGG + chr4_3:6018245-6018264 Msa0626440:five_prime_UTR 40.0%
ATCATCCACAATATTCAGCC+AGG + chr4_3:6018283-6018302 Msa0626440:five_prime_UTR 40.0%
TCATCCACAATATTCAGCCA+GGG + chr4_3:6018284-6018303 Msa0626440:five_prime_UTR 40.0%
GAACCGATGCAGAATGAAAT+AGG - chr4_3:6018384-6018403 None:intergenic 40.0%
!! CCGTATAATGGATGATCCTT+CGG - chr4_3:6018621-6018640 None:intergenic 40.0%
CCGAAGGATCATCCATTATA+CGG + chr4_3:6018618-6018637 Msa0626440:CDS 40.0%
AGAAAGCGACAACCGTATAA+TGG - chr4_3:6018633-6018652 None:intergenic 40.0%
AGATCTTCGCGAAGCTAAAT+TGG + chr4_3:6018737-6018756 Msa0626440:CDS 40.0%
CTGTGAATATTGTTGGACTC+TGG + chr4_3:6018837-6018856 Msa0626440:intron 40.0%
CAAAAGAACCAACTGCGAAA+AGG + chr4_3:6018927-6018946 Msa0626440:CDS 40.0%
AAAAGGGCTAAAGTTGGGAA+AGG + chr4_3:6018944-6018963 Msa0626440:CDS 40.0%
AAAGGGCTAAAGTTGGGAAA+GGG + chr4_3:6018945-6018964 Msa0626440:CDS 40.0%
GGGCTTCTATGAATTGTGTT+TGG + chr4_3:6019020-6019039 Msa0626440:intron 40.0%
! TTTAAAATCGAAGCAGCAGG+AGG + chr4_3:6019109-6019128 Msa0626440:intron 40.0%
TCACAAATCTTCTTTGCTGC+AGG - chr4_3:6019176-6019195 None:intergenic 40.0%
AGGAGAAGAACGAACCATTT+GGG - chr4_3:6019305-6019324 None:intergenic 40.0%
AAGGAGAAGAACGAACCATT+TGG - chr4_3:6019306-6019325 None:intergenic 40.0%
!! GTAATTATAAGTGTCGGGGT+TGG + chr4_3:6019407-6019426 Msa0626440:intron 40.0%
TATGAAGCATGGACACTTCT+CGG - chr4_3:6019454-6019473 None:intergenic 40.0%
AGCCTTGAAGCCAAAATCAA+AGG + chr4_3:6019635-6019654 Msa0626440:CDS 40.0%
GGGAAAAAATTTCACCTCTC+GGG - chr4_3:6019805-6019824 None:intergenic 40.0%
GGGGAAAAAATTTCACCTCT+CGG - chr4_3:6019806-6019825 None:intergenic 40.0%
CAAAAGAACCAACTGCGAAA+AGG + chr4_3:6018927-6018946 Msa0626440:CDS 40.0%
!!! TTTGCTCTGTTTTCTGAAGC+TGG + chr4_3:6020010-6020029 Msa0626440:intron 40.0%
AGTTAACAGATGAGGAGTAG+TGG + chr4_3:6020130-6020149 Msa0626440:CDS 40.0%
!! CTATTGAACCGGCTTGATTT+AGG + chr4_3:6020185-6020204 Msa0626440:three_prime_UTR 40.0%
! AGCTTTAGCCTAAATCAAGC+CGG - chr4_3:6020196-6020215 None:intergenic 40.0%
! ATATCTTTTCGCACTGTCCT+TGG + chr4_3:6020278-6020297 Msa0626440:three_prime_UTR 40.0%
CAGGCATTAGCAATATTCCA+AGG - chr4_3:6020298-6020317 None:intergenic 40.0%
ATGATCGCAGGATATAGAAG+AGG - chr4_3:6020339-6020358 None:intergenic 40.0%
TAACTGAGGGATCTGTTAGA+TGG + chr4_3:6020443-6020462 Msa0626440:three_prime_UTR 40.0%
AACTGAGGGATCTGTTAGAT+GGG + chr4_3:6020444-6020463 Msa0626440:three_prime_UTR 40.0%
TCCTACTTAACCAGATGCTT+TGG + chr4_3:6020499-6020518 Msa0626440:three_prime_UTR 40.0%
! ACCAAAGCATCTGGTTAAGT+AGG - chr4_3:6020503-6020522 None:intergenic 40.0%
CAAAATAGACCATCAGGGAT+AGG - chr4_3:6020652-6020671 None:intergenic 40.0%
ACAATATTCAGCCAGGGCTA+CGG + chr4_3:6018290-6018309 Msa0626440:five_prime_UTR 45.0%
ATATTCGGACGATGCACTCT+TGG + chr4_3:6018659-6018678 Msa0626440:CDS 45.0%
CTGAAGTGAAGAAACCTGAG+AGG + chr4_3:6018802-6018821 Msa0626440:CDS 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - chr4_3:6018938-6018957 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + chr4_3:6018938-6018957 Msa0626440:CDS 45.0%
CTGCGAAAAGGGCTAAAGTT+GGG + chr4_3:6018939-6018958 Msa0626440:CDS 45.0%
GGAGAAGAACGAACCATTTG+GGG - chr4_3:6019304-6019323 None:intergenic 45.0%
TCCTTCTCTTCTACTGACAC+CGG + chr4_3:6019321-6019340 Msa0626440:intron 45.0%
!! GAGAAGTGTCCATGCTTCAT+AGG + chr4_3:6019453-6019472 Msa0626440:intron 45.0%
GATCAGAGTCCTATGAAGCA+TGG - chr4_3:6019465-6019484 None:intergenic 45.0%
CTACAATTGTGCGTGTCAAC+GGG - chr4_3:6019617-6019636 None:intergenic 45.0%
GCTACAATTGTGCGTGTCAA+CGG - chr4_3:6019618-6019637 None:intergenic 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - chr4_3:6018938-6018957 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + chr4_3:6018938-6018957 Msa0626440:CDS 45.0%
GGATCAGGAGGGATATGATA+AGG + chr4_3:6020050-6020069 Msa0626440:intron 45.0%
GGAAGGCCAATCTGATGATA+AGG + chr4_3:6020080-6020099 Msa0626440:CDS 45.0%
GGGCTTCCTTATCATCAGAT+TGG - chr4_3:6020089-6020108 None:intergenic 45.0%
GGTGGAAGAGTTAACAGATG+AGG + chr4_3:6020122-6020141 Msa0626440:CDS 45.0%
! GAGGAGTAGTGGTAGCATTT+TGG + chr4_3:6020141-6020160 Msa0626440:exon 45.0%
GGCAAGTTTAACATGATCGC+AGG - chr4_3:6020351-6020370 None:intergenic 45.0%
TGGGCAATGACTTCTGTGAA+TGG + chr4_3:6020463-6020482 Msa0626440:three_prime_UTR 45.0%
ACGATTGATCCTATCCCTGA+TGG + chr4_3:6020640-6020659 Msa0626440:three_prime_UTR 45.0%
GTAGCCCTGGCTGAATATTG+TGG - chr4_3:6018291-6018310 None:intergenic 50.0%
!! CAATGAAAGTACCGTAGCCC+TGG - chr4_3:6018304-6018323 None:intergenic 50.0%
GTGACAAAGATGGCCCCAAA+TGG + chr4_3:6019288-6019307 Msa0626440:CDS 50.0%
! TCCGGTGTCAGTAGAAGAGA+AGG - chr4_3:6019325-6019344 None:intergenic 50.0%
GATTGTGTCCATGTCGTGTC+CGG - chr4_3:6019343-6019362 None:intergenic 50.0%
GCATGGACACTTCTCGGATT+AGG - chr4_3:6019448-6019467 None:intergenic 50.0%
CTGAAGTGAAGAAACCCGAG+AGG + chr4_3:6019788-6019807 Msa0626440:CDS 50.0%
GTTAACTCTTCCACCTCTCC+TGG - chr4_3:6020117-6020136 None:intergenic 50.0%
ATTCGCTGCGCTCACTAGTT+CGG + chr4_3:6020676-6020695 Msa0626440:three_prime_UTR 50.0%
CAACTCCAAGTCCATGGTGG+CGG - chr4_3:6018598-6018617 None:intergenic 55.0%
CTTCGGCAACTCCAAGTCCA+TGG - chr4_3:6018604-6018623 None:intergenic 55.0%
CATGGACTTGGAGTTGCCGA+AGG + chr4_3:6018602-6018621 Msa0626440:exon 55.0%
TACTGACACCGGACACGACA+TGG + chr4_3:6019332-6019351 Msa0626440:intron 55.0%
! TTCTCGGATTAGGCGTGTCC+CGG - chr4_3:6019438-6019457 None:intergenic 55.0%
AGAAACCCTTGCCGCCACCA+TGG + chr4_3:6018584-6018603 Msa0626440:intron 60.0%
!! CAAGTCCATGGTGGCGGCAA+GGG - chr4_3:6018592-6018611 None:intergenic 60.0%
CGGCAACTCCAAGTCCATGG+TGG - chr4_3:6018601-6018620 None:intergenic 60.0%
! AGTGTCGGGGTTGGACACAC+CGG + chr4_3:6019416-6019435 Msa0626440:intron 60.0%
TGCGTGTCAACGGGTGCAAC+AGG - chr4_3:6019608-6019627 None:intergenic 60.0%
GATAAGGAAGCCCCTGCACC+AGG + chr4_3:6020096-6020115 Msa0626440:CDS 60.0%
TTCCACCTCTCCTGGTGCAG+GGG - chr4_3:6020109-6020128 None:intergenic 60.0%
CTTCCACCTCTCCTGGTGCA+GGG - chr4_3:6020110-6020129 None:intergenic 60.0%
TCTTCCACCTCTCCTGGTGC+AGG - chr4_3:6020111-6020130 None:intergenic 60.0%
!! CCAAGTCCATGGTGGCGGCA+AGG - chr4_3:6018593-6018612 None:intergenic 65.0%
CCTTGCCGCCACCATGGACT+TGG + chr4_3:6018590-6018609 Msa0626440:exon 65.0%
GTGTCGGGGTTGGACACACC+GGG + chr4_3:6019417-6019436 Msa0626440:intron 65.0%
GGAAGCCCCTGCACCAGGAG+AGG + chr4_3:6020101-6020120 Msa0626440:CDS 70.0%
AGCCCCTGCACCAGGAGAGG+TGG + chr4_3:6020104-6020123 Msa0626440:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 6018100 6020840 6018100 ID=Msa0626440;Name=Msa0626440
chr4_3 mRNA 6018100 6020840 6018100 ID=Msa0626440-mRNA-1;Parent=Msa0626440;Name=Msa0626440-mRNA-1;_AED=0.01;_eAED=0.01;_QI=229|0.85|0.87|1|0.85|0.75|8|691|254
chr4_3 exon 6018100 6018311 6018100 ID=Msa0626440-mRNA-1:exon:1708;Parent=Msa0626440-mRNA-1
chr4_3 exon 6018586 6018823 6018586 ID=Msa0626440-mRNA-1:exon:1709;Parent=Msa0626440-mRNA-1
chr4_3 exon 6018913 6019000 6018913 ID=Msa0626440-mRNA-1:exon:1710;Parent=Msa0626440-mRNA-1
chr4_3 exon 6019122 6019309 6019122 ID=Msa0626440-mRNA-1:exon:1711;Parent=Msa0626440-mRNA-1
chr4_3 exon 6019583 6019656 6019583 ID=Msa0626440-mRNA-1:exon:1712;Parent=Msa0626440-mRNA-1
chr4_3 exon 6019764 6019809 6019764 ID=Msa0626440-mRNA-1:exon:1713;Parent=Msa0626440-mRNA-1
chr4_3 exon 6019906 6019954 6019906 ID=Msa0626440-mRNA-1:exon:1714;Parent=Msa0626440-mRNA-1
chr4_3 exon 6020051 6020840 6020051 ID=Msa0626440-mRNA-1:exon:1715;Parent=Msa0626440-mRNA-1
chr4_3 five_prime_UTR 6018100 6018311 6018100 ID=Msa0626440-mRNA-1:five_prime_utr;Parent=Msa0626440-mRNA-1
chr4_3 five_prime_UTR 6018586 6018602 6018586 ID=Msa0626440-mRNA-1:five_prime_utr;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6018603 6018823 6018603 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6018913 6019000 6018913 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6019122 6019309 6019122 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6019583 6019656 6019583 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6019764 6019809 6019764 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6019906 6019954 6019906 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 CDS 6020051 6020149 6020051 ID=Msa0626440-mRNA-1:cds;Parent=Msa0626440-mRNA-1
chr4_3 three_prime_UTR 6020150 6020840 6020150 ID=Msa0626440-mRNA-1:three_prime_utr;Parent=Msa0626440-mRNA-1
Gene Sequence

>Msa0626440

ATGGACTTGGAGTTGCCGAAGGATCATCCATTATACGGTTGTCGCTTTCTAAATTTATATTCGGACGATGCACTCTTGGAGTTATATAAGTACGAAGATATGGTGGACGCTAAATTTGATCTGTGTCTTTTTGAAGATCTTCGCGAAGCTAAATTGGAAAAAGATGAACAAAATCTTTGTTTAGATTCTGAGCAAGCTTCTGAAGTGAAGAAACCTGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGGAAAGGGAAAAATGTTAATGAATCTAATGCTGAAAATGAACAGCAGGAGGCTTGTGCAGTATACCATGTTTTTGAAGAAATGATTCAGTTACCTGCAGCAAAGAAGATTTGTGATGAGCATAATGTTGATGAAAAAATTCTCACAAAAGTCTTGGAGAATTTGAAGAATTCCATTACCCAAGAAGCTTTGATTCAACTAGAAATAAGTGACAAAGATGGCCCCAAATGTGATGAGAGTAAGAAAGATGTTCCTGTTGCACCCGTTGACACGCACAATTGTAGCCTTGAAGCCAAAATCAAAGATTCTCATGCTGTTGAGCAAGCTGCTGAAGTGAAGAAACCCGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGAGATCAGGAGGGATATGATAAGGAAGATAAGGAAGGCCAATCTGATGATAAGGAAGCCCCTGCACCAGGAGAGGTGGAAGAGTTAACAGATGAGGAGTAG
Protein sequence

>Msa0626440

MDLELPKDHPLYGCRFLNLYSDDALLELYKYEDMVDAKFDLCLFEDLREAKLEKDEQNLCLDSEQASEVKKPERKKPNSKEPTAKRAKVGKGKNVNESNAENEQQEACAVYHVFEEMIQLPAAKKICDEHNVDEKILTKVLENLKNSITQEALIQLEISDKDGPKCDESKKDVPVAPVDTHNCSLEAKIKDSHAVEQAAEVKKPERKKPNSKEPTAKRAKVGDQEGYDKEDKEGQSDDKEAPAPGEVEELTDEE*