AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0626480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0626480 MtrunA17_Chr8g0342651 61.905 126 13 1 103 228 88 178 1.65e-40 139
Msa0626480 MtrunA17_Chr8g0342651 49.351 77 31 2 64 140 149 217 5.02e-11 60.8
Msa0626480 MtrunA17_Chr1g0212341 80.882 68 10 1 158 222 21 88 7.71e-28 105
Msa0626480 MtrunA17_Chr5g0438531 55.932 59 22 1 104 158 69 127 4.57e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCATTACTTACATTATTT+TGG 0.125240 4_3:+6068521 Msa0626480:three_prime_UTR
ATTATGCAATGTTACTTTAT+TGG 0.155712 4_3:-6068777 None:intergenic
ACGATGATTACCTTTGATTT+TGG 0.169382 4_3:-6067628 None:intergenic
TGTCATCCTTCAATTATATT+TGG 0.184257 4_3:+6066167 Msa0626480:five_prime_UTR
TTGGATTGTTATGTGATATT+AGG 0.202665 4_3:-6065902 None:intergenic
AGATCTTCGCGAAGCTAAAT+TGG 0.247966 4_3:+6066719 Msa0626480:CDS
CTATTGAACCGGCTTGATTT+AGG 0.266233 4_3:+6068168 Msa0626480:three_prime_UTR
GAACACAAGCAGTTCATAAT+TGG 0.276454 4_3:-6068811 None:intergenic
CTAGTTGAATCAAAGCTTCT+TGG 0.282657 4_3:-6067239 None:intergenic
GAGGAGTAGTGGTAGCATTT+TGG 0.290072 4_3:+6068124 Msa0626480:exon
GTCATCCTTCAATTATATTT+GGG 0.305842 4_3:+6066168 Msa0626480:five_prime_UTR
AACTTGCCAAATGCATTATC+AGG 0.326793 4_3:+6068363 Msa0626480:three_prime_UTR
TCGCTTTCTAAATTTATATT+CGG 0.331894 4_3:+6066626 Msa0626480:CDS
AATAAAGTAACATTGCATAA+TGG 0.337041 4_3:+6068779 Msa0626480:three_prime_UTR
ATAAAGTAACATTGCATAAT+GGG 0.338506 4_3:+6068780 Msa0626480:three_prime_UTR
TATGTAACGAAATAATAAAT+TGG 0.353966 4_3:-6065921 None:intergenic
TAACTGAGGGATCTGTTAGA+TGG 0.365103 4_3:+6068443 Msa0626480:three_prime_UTR
TCCTACTTAACCAGATGCTT+TGG 0.368865 4_3:+6068499 Msa0626480:three_prime_UTR
TGACCCTTGTTTAAAATTGA+AGG 0.371260 4_3:-6065950 None:intergenic
CCGAAGGATCATCCATTATA+CGG 0.375242 4_3:+6066600 Msa0626480:CDS
CTGAAACCTGATAATGCATT+TGG 0.390036 4_3:-6068369 None:intergenic
TGCGTGTCAACGGGTGCAAC+AGG 0.392472 4_3:-6067588 None:intergenic
AGGAGAAGAACGAACCATTT+GGG 0.393055 4_3:-6067291 None:intergenic
AATCAAAGCTTCTTGGGTAA+TGG 0.393707 4_3:-6067232 None:intergenic
TCTTCCACCTCTCCTGGTGC+AGG 0.394878 4_3:-6068091 None:intergenic
ATTCGCTGCGCTCACTAGTT+CGG 0.405936 4_3:+6068676 Msa0626480:three_prime_UTR
AGAAAGCGACAACCGTATAA+TGG 0.406074 4_3:-6066612 None:intergenic
ACTTGATTTCTCTATTGAAC+CGG 0.410703 4_3:+6068157 Msa0626480:three_prime_UTR
TTCGACCCAAATATAATTGA+AGG 0.423577 4_3:-6066173 None:intergenic
GGGATATGATAAGGAAGATA+AGG 0.437427 4_3:+6068042 Msa0626480:CDS
CTACAATTGTGCGTGTCAAC+GGG 0.442078 4_3:-6067597 None:intergenic
ATGGCAGTGCTTTCCCCAAA+TGG 0.446026 4_3:+6067277 Msa0626480:CDS
CTTCCACCTCTCCTGGTGCA+GGG 0.450689 4_3:-6068090 None:intergenic
GTTAACTCTTCCACCTCTCC+TGG 0.451248 4_3:-6068097 None:intergenic
AGACTAAAATTTCACCTCTC+AGG 0.451846 4_3:-6066798 None:intergenic
ATATTACAAAATAGACCATC+AGG 0.453264 4_3:-6068655 None:intergenic
CCTTGCCGCCACCATGGACT+TGG 0.453782 4_3:+6066572 Msa0626480:exon
ATACACACACACAAGTGCTT+TGG 0.457560 4_3:+6066231 Msa0626480:five_prime_UTR
CCGTATAATGGATGATCCTT+CGG 0.457729 4_3:-6066600 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4_3:+6066907 Msa0626480:CDS
TAGTTGAATCAAAGCTTCTT+GGG 0.458718 4_3:-6067238 None:intergenic
AAAAGAACCAACTGCGAAAA+GGG 0.458718 4_3:+6067904 Msa0626480:CDS
ATATTCGGACGAGGCACTCT+TGG 0.459564 4_3:+6066641 Msa0626480:CDS
TCACAAATCTTCTTTGCTGC+AGG 0.460278 4_3:-6067153 None:intergenic
CAAGTCCATGGTGGCGGCAA+GGG 0.461283 4_3:-6066571 None:intergenic
GGATCAGGAGGGATATGATA+AGG 0.465606 4_3:+6068033 Msa0626480:intron
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4_3:+6066906 Msa0626480:CDS
CAAAAGAACCAACTGCGAAA+AGG 0.465631 4_3:+6067903 Msa0626480:CDS
GGAAGGCCAATCTGATGATA+AGG 0.467371 4_3:+6068063 Msa0626480:CDS
AAGTAATGAACCAAAGCATC+TGG 0.474408 4_3:-6068509 None:intergenic
GCTACAATTGTGCGTGTCAA+CGG 0.476888 4_3:-6067598 None:intergenic
AGCCCCTGCACCAGGAGAGG+TGG 0.481468 4_3:+6068087 Msa0626480:CDS
GGGCTTCCTTATCATCAGAT+TGG 0.481597 4_3:-6068069 None:intergenic
TTCCACCTCTCCTGGTGCAG+GGG 0.483421 4_3:-6068089 None:intergenic
CTAGTAGAAAATAATGTAGC+AGG 0.490160 4_3:-6068297 None:intergenic
ATCATCCACAATATTCAGCC+AGG 0.493124 4_3:+6066269 Msa0626480:five_prime_UTR
GTTATATAAGTACGAAGATA+AGG 0.511157 4_3:+6066665 Msa0626480:CDS
AGTTAACAGATGAGGAGTAG+TGG 0.515594 4_3:+6068113 Msa0626480:CDS
GATAAGGAAGCCCCTGCACC+AGG 0.518868 4_3:+6068079 Msa0626480:CDS
CTGCGAAAAGGGCTAAAGTT+GGG 0.522171 4_3:+6066918 Msa0626480:CDS
ACGATTGATCCTATCCCTGA+TGG 0.525163 4_3:+6068640 Msa0626480:three_prime_UTR
GGCAAGTTTAACATGATCGC+AGG 0.528195 4_3:-6068348 None:intergenic
CAATGAAAGTACCGTAGCCC+TGG 0.537406 4_3:-6066287 None:intergenic
ACCAAAGCATCTGGTTAAGT+AGG 0.541232 4_3:-6068500 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.541934 4_3:+6066917 Msa0626480:CDS
CCAAGTCCATGGTGGCGGCA+AGG 0.543580 4_3:-6066572 None:intergenic
AACTGAGGGATCTGTTAGAT+GGG 0.547128 4_3:+6068444 Msa0626480:three_prime_UTR
CAAAATAGACCATCAGGGAT+AGG 0.548322 4_3:-6068649 None:intergenic
CAACTCCAAGTCCATGGTGG+CGG 0.554736 4_3:-6066577 None:intergenic
ACTGCGAAAAGGGCTAAAGT+TGG 0.558971 4_3:+6067914 Msa0626480:CDS
ACAATATTCAGCCAGGGCTA+CGG 0.567396 4_3:+6066276 Msa0626480:five_prime_UTR
TGGGCAATGACTTCTGTGAA+TGG 0.568132 4_3:+6068463 Msa0626480:three_prime_UTR
GGAAGCCCCTGCACCAGGAG+AGG 0.576902 4_3:+6068084 Msa0626480:CDS
GGTGGAAGAGTTAACAGATG+AGG 0.581307 4_3:+6068105 Msa0626480:CDS
AAAAGGGCTAAAGTTGGGAA+AGG 0.581404 4_3:+6066923 Msa0626480:CDS
AGAAACCCTTGCCGCCACCA+TGG 0.582020 4_3:+6066566 Msa0626480:intron
CTTCGGCAACTCCAAGTCCA+TGG 0.589099 4_3:-6066583 None:intergenic
TATTTATGTTTAGGATCAGG+AGG 0.589442 4_3:+6068021 Msa0626480:intron
TCTAAATTTATATTCGGACG+AGG 0.591042 4_3:+6066632 Msa0626480:CDS
AGCTTTAGCCTAAATCAAGC+CGG 0.591356 4_3:-6068176 None:intergenic
GTAGCCCTGGCTGAATATTG+TGG 0.592581 4_3:-6066274 None:intergenic
AGCCTTGAAGCCAAAATCAA+AGG 0.601595 4_3:+6067618 Msa0626480:CDS
CATGGACTTGGAGTTGCCGA+AGG 0.601960 4_3:+6066584 Msa0626480:exon
CTAGAAATAAGTGACAAAGA+TGG 0.605930 4_3:+6067258 Msa0626480:CDS
ATTTATGTTTAGGATCAGGA+GGG 0.610871 4_3:+6068022 Msa0626480:intron
AAAGGGCTAAAGTTGGGAAA+GGG 0.618574 4_3:+6066924 Msa0626480:CDS
TATGATAAGGAAGATAAGGA+AGG 0.621270 4_3:+6068046 Msa0626480:CDS
TCATCCACAATATTCAGCCA+GGG 0.650109 4_3:+6066270 Msa0626480:five_prime_UTR
CAGGCATTAGCAATATTCCA+AGG 0.651084 4_3:-6068278 None:intergenic
ATGATCGCAGGATATAGAAG+AGG 0.655857 4_3:-6068336 None:intergenic
GGAGAAGAACGAACCATTTG+GGG 0.661729 4_3:-6067290 None:intergenic
CTGAAGTGAAGAAACCTGAG+AGG 0.670696 4_3:+6066784 Msa0626480:CDS
CGGCAACTCCAAGTCCATGG+TGG 0.682013 4_3:-6066580 None:intergenic
TATTACAAAATAGACCATCA+GGG 0.682398 4_3:-6068654 None:intergenic
ATATAAGTACGAAGATAAGG+TGG 0.691646 4_3:+6066668 Msa0626480:CDS
CTGAAGTGAAGAAACCCGAG+AGG 0.719762 4_3:+6067771 Msa0626480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGGTTTATTTATTTATTT+TGG + chr4_3:6066859-6066878 Msa0626480:intron 10.0%
!!! GTATTTGATTTTATTTAATT+TGG + chr4_3:6067716-6067735 Msa0626480:intron 10.0%
!! TATGTAACGAAATAATAAAT+TGG - chr4_3:6065924-6065943 None:intergenic 15.0%
!!! GTTTTTTTTTGTTAGTATTT+AGG + chr4_3:6066841-6066860 Msa0626480:intron 15.0%
!!! TATTTATTTTGGAATTTTGT+AGG + chr4_3:6066870-6066889 Msa0626480:intron 15.0%
!!! AAGTATGTTTTCTTTTAATT+TGG + chr4_3:6066978-6066997 Msa0626480:intron 15.0%
!!! TTGATAATAATTCCTAATTA+GGG - chr4_3:6067667-6067686 None:intergenic 15.0%
!!! TTTGATAATAATTCCTAATT+AGG - chr4_3:6067668-6067687 None:intergenic 15.0%
!! AATATTCACAAACTAAAATT+GGG - chr4_3:6067809-6067828 None:intergenic 15.0%
!!! TTTAGGTTTATCTATTTTTT+TGG + chr4_3:6067856-6067875 Msa0626480:intron 15.0%
!!! TTATAACTTTACTTTTGTAT+CGG + chr4_3:6068551-6068570 Msa0626480:three_prime_UTR 15.0%
!!! TTTAGTTGCTAATTTTTTAT+TGG + chr4_3:6068605-6068624 Msa0626480:three_prime_UTR 15.0%
!!! TTAGTTGCTAATTTTTTATT+GGG + chr4_3:6068606-6068625 Msa0626480:three_prime_UTR 15.0%
!!! TATACCTTCAATTTTAAACA+AGG + chr4_3:6065946-6065965 Msa0626480:intron 20.0%
!!! ATACCTTCAATTTTAAACAA+GGG + chr4_3:6065947-6065966 Msa0626480:intron 20.0%
!! GTTTGTGTTAATTGTTTAAA+GGG - chr4_3:6066142-6066161 None:intergenic 20.0%
!! AGTTTGTGTTAATTGTTTAA+AGG - chr4_3:6066143-6066162 None:intergenic 20.0%
!!! TTTTGAAGTGTTTATTAACA+AGG - chr4_3:6066206-6066225 None:intergenic 20.0%
!! AATCAATTTATGTCGAAAAA+CGG + chr4_3:6066425-6066444 Msa0626480:intron 20.0%
!! TCGCTTTCTAAATTTATATT+CGG + chr4_3:6066626-6066645 Msa0626480:CDS 20.0%
!! ATATTCACAAACTAAAATTG+GGG - chr4_3:6067808-6067827 None:intergenic 20.0%
!! CAATATTCACAAACTAAAAT+TGG - chr4_3:6067810-6067829 None:intergenic 20.0%
!!! CAATTTTAGTTTGTGAATAT+TGG + chr4_3:6067808-6067827 Msa0626480:intron 20.0%
!!! CTATTTTTTTGGAATTTTGT+AGG + chr4_3:6067867-6067886 Msa0626480:intron 20.0%
!!! GTTCATTACTTACATTATTT+TGG + chr4_3:6068521-6068540 Msa0626480:three_prime_UTR 20.0%
!!! TACTTACATTATTTTGGATT+AGG + chr4_3:6068527-6068546 Msa0626480:three_prime_UTR 20.0%
!!! TATAACTTTACTTTTGTATC+GGG + chr4_3:6068552-6068571 Msa0626480:three_prime_UTR 20.0%
!!! ATTATGCAATGTTACTTTAT+TGG - chr4_3:6068780-6068799 None:intergenic 20.0%
!! AATAAAGTAACATTGCATAA+TGG + chr4_3:6068779-6068798 Msa0626480:three_prime_UTR 20.0%
!! ATAAAGTAACATTGCATAAT+GGG + chr4_3:6068780-6068799 Msa0626480:three_prime_UTR 20.0%
! TTGGATTGTTATGTGATATT+AGG - chr4_3:6065905-6065924 None:intergenic 25.0%
! TGTCATCCTTCAATTATATT+TGG + chr4_3:6066167-6066186 Msa0626480:five_prime_UTR 25.0%
! GTCATCCTTCAATTATATTT+GGG + chr4_3:6066168-6066187 Msa0626480:five_prime_UTR 25.0%
! TTCATATTTCGAAAAACCTA+AGG + chr4_3:6066393-6066412 Msa0626480:intron 25.0%
! AAAACGGTTATTCTTTGTAT+TGG + chr4_3:6066441-6066460 Msa0626480:intron 25.0%
! GTTATATAAGTACGAAGATA+AGG + chr4_3:6066665-6066684 Msa0626480:CDS 25.0%
!! TTTTAGTCTGTGAATATTGT+TGG + chr4_3:6066812-6066831 Msa0626480:intron 25.0%
! GAATCATTTCTTCAAAAACA+TGG - chr4_3:6067128-6067147 None:intergenic 25.0%
! AAAAATTCTCACAAAAGTCT+TGG + chr4_3:6067194-6067213 Msa0626480:CDS 25.0%
! TTTCAAATTATTAGCTGTGT+CGG - chr4_3:6067358-6067377 None:intergenic 25.0%
! ATTACTCATAAACCCTAATT+AGG + chr4_3:6067652-6067671 Msa0626480:intron 25.0%
!! TCTTGTTTGTGTTAGTATTT+AGG + chr4_3:6067833-6067852 Msa0626480:intron 25.0%
!! TTGTGTTAGTATTTAGGTTT+AGG + chr4_3:6067839-6067858 Msa0626480:intron 25.0%
!! CTATATTTATGTTTAGGATC+AGG + chr4_3:6068018-6068037 Msa0626480:intron 25.0%
!!! ATAACTTTACTTTTGTATCG+GGG + chr4_3:6068553-6068572 Msa0626480:three_prime_UTR 25.0%
! TATTACAAAATAGACCATCA+GGG - chr4_3:6068657-6068676 None:intergenic 25.0%
! ATATTACAAAATAGACCATC+AGG - chr4_3:6068658-6068677 None:intergenic 25.0%
! TGACCCTTGTTTAAAATTGA+AGG - chr4_3:6065953-6065972 None:intergenic 30.0%
TTCGACCCAAATATAATTGA+AGG - chr4_3:6066176-6066195 None:intergenic 30.0%
TTACCTATTTCATTCTGCAT+CGG + chr4_3:6066364-6066383 Msa0626480:intron 30.0%
!! ATTGATTCGTTTACAACCTT+AGG - chr4_3:6066412-6066431 None:intergenic 30.0%
TCTAAATTTATATTCGGACG+AGG + chr4_3:6066632-6066651 Msa0626480:CDS 30.0%
ATATAAGTACGAAGATAAGG+TGG + chr4_3:6066668-6066687 Msa0626480:CDS 30.0%
TACCAAACACAATTCATAGA+AGG - chr4_3:6067004-6067023 None:intergenic 30.0%
TAGTTGAATCAAAGCTTCTT+GGG - chr4_3:6067241-6067260 None:intergenic 30.0%
CTAGAAATAAGTGACAAAGA+TGG + chr4_3:6067258-6067277 Msa0626480:CDS 30.0%
TTGAGTGTAATTGTAAGTGT+CGG + chr4_3:6067390-6067409 Msa0626480:intron 30.0%
!! ACGATGATTACCTTTGATTT+TGG - chr4_3:6067631-6067650 None:intergenic 30.0%
! AGTTGGAGTATGTTTTCTTT+TGG + chr4_3:6067931-6067950 Msa0626480:intron 30.0%
! GTTGGAGTATGTTTTCTTTT+GGG + chr4_3:6067932-6067951 Msa0626480:intron 30.0%
!! CTGGTGCTATATTTATGTTT+AGG + chr4_3:6068012-6068031 Msa0626480:intron 30.0%
! TATTTATGTTTAGGATCAGG+AGG + chr4_3:6068021-6068040 Msa0626480:intron 30.0%
! ATTTATGTTTAGGATCAGGA+GGG + chr4_3:6068022-6068041 Msa0626480:intron 30.0%
TATGATAAGGAAGATAAGGA+AGG + chr4_3:6068046-6068065 Msa0626480:CDS 30.0%
ACTTGATTTCTCTATTGAAC+CGG + chr4_3:6068157-6068176 Msa0626480:three_prime_UTR 30.0%
CTAGTAGAAAATAATGTAGC+AGG - chr4_3:6068300-6068319 None:intergenic 30.0%
! TTCTTTTCTGCATGTTTACT+AGG + chr4_3:6068409-6068428 Msa0626480:three_prime_UTR 30.0%
! TCTTTTCTGCATGTTTACTA+GGG + chr4_3:6068410-6068429 Msa0626480:three_prime_UTR 30.0%
GGTTAAGGGTCAAATATAGT+AGG - chr4_3:6066091-6066110 None:intergenic 35.0%
AGACTAAAATTTCACCTCTC+AGG - chr4_3:6066801-6066820 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - chr4_3:6066902-6066921 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + chr4_3:6066907-6066926 Msa0626480:CDS 35.0%
TGTTTAAAAATCGAAGCAGC+AGG + chr4_3:6067086-6067105 Msa0626480:intron 35.0%
AATCAAAGCTTCTTGGGTAA+TGG - chr4_3:6067235-6067254 None:intergenic 35.0%
CTAGTTGAATCAAAGCTTCT+TGG - chr4_3:6067242-6067261 None:intergenic 35.0%
TGAGTGTAATTGTAAGTGTC+GGG + chr4_3:6067391-6067410 Msa0626480:intron 35.0%
!!! TACCTTTGATTTTGGCTTCA+AGG - chr4_3:6067623-6067642 None:intergenic 35.0%
!! GCAGTTGGTTCTTTTGAATT+TGG - chr4_3:6066902-6066921 None:intergenic 35.0%
AAAAGAACCAACTGCGAAAA+GGG + chr4_3:6066907-6066926 Msa0626480:CDS 35.0%
GGGATATGATAAGGAAGATA+AGG + chr4_3:6068042-6068061 Msa0626480:CDS 35.0%
AACTTGCCAAATGCATTATC+AGG + chr4_3:6068363-6068382 Msa0626480:three_prime_UTR 35.0%
! CTGAAACCTGATAATGCATT+TGG - chr4_3:6068372-6068391 None:intergenic 35.0%
! CTTTTCTGCATGTTTACTAG+GGG + chr4_3:6068411-6068430 Msa0626480:three_prime_UTR 35.0%
! AGGGGAAATTTTCTTAACTG+AGG + chr4_3:6068429-6068448 Msa0626480:three_prime_UTR 35.0%
! GGGGAAATTTTCTTAACTGA+GGG + chr4_3:6068430-6068449 Msa0626480:three_prime_UTR 35.0%
! TGGTTAAGTAGGATTTTCTC+TGG - chr4_3:6068492-6068511 None:intergenic 35.0%
! AAGTAATGAACCAAAGCATC+TGG - chr4_3:6068512-6068531 None:intergenic 35.0%
!!! GGTATTTTAGCTCACTGTTT+TGG + chr4_3:6068697-6068716 Msa0626480:three_prime_UTR 35.0%
GAACACAAGCAGTTCATAAT+TGG - chr4_3:6068814-6068833 None:intergenic 35.0%
ATACACACACACAAGTGCTT+TGG + chr4_3:6066231-6066250 Msa0626480:five_prime_UTR 40.0%
ATCATCCACAATATTCAGCC+AGG + chr4_3:6066269-6066288 Msa0626480:five_prime_UTR 40.0%
TCATCCACAATATTCAGCCA+GGG + chr4_3:6066270-6066289 Msa0626480:five_prime_UTR 40.0%
GAACCGATGCAGAATGAAAT+AGG - chr4_3:6066370-6066389 None:intergenic 40.0%
!! CCGTATAATGGATGATCCTT+CGG - chr4_3:6066603-6066622 None:intergenic 40.0%
CCGAAGGATCATCCATTATA+CGG + chr4_3:6066600-6066619 Msa0626480:CDS 40.0%
AGAAAGCGACAACCGTATAA+TGG - chr4_3:6066615-6066634 None:intergenic 40.0%
AGATCTTCGCGAAGCTAAAT+TGG + chr4_3:6066719-6066738 Msa0626480:CDS 40.0%
CTGTGAATATTGTTGGACTC+TGG + chr4_3:6066819-6066838 Msa0626480:intron 40.0%
CAAAAGAACCAACTGCGAAA+AGG + chr4_3:6066906-6066925 Msa0626480:CDS 40.0%
AAAAGGGCTAAAGTTGGGAA+AGG + chr4_3:6066923-6066942 Msa0626480:CDS 40.0%
AAAGGGCTAAAGTTGGGAAA+GGG + chr4_3:6066924-6066943 Msa0626480:CDS 40.0%
GGCCTTCTATGAATTGTGTT+TGG + chr4_3:6066999-6067018 Msa0626480:intron 40.0%
TTAAAAATCGAAGCAGCAGG+AGG + chr4_3:6067089-6067108 Msa0626480:intron 40.0%
TCACAAATCTTCTTTGCTGC+AGG - chr4_3:6067156-6067175 None:intergenic 40.0%
AGGAGAAGAACGAACCATTT+GGG - chr4_3:6067294-6067313 None:intergenic 40.0%
AAGGAGAAGAACGAACCATT+TGG - chr4_3:6067295-6067314 None:intergenic 40.0%
! GAGTGTAATTGTAAGTGTCG+GGG + chr4_3:6067392-6067411 Msa0626480:intron 40.0%
TATGAAGCATGGACACTTCT+CGG - chr4_3:6067427-6067446 None:intergenic 40.0%
AGCCTTGAAGCCAAAATCAA+AGG + chr4_3:6067618-6067637 Msa0626480:CDS 40.0%
GGGAAAAAATTTCACCTCTC+GGG - chr4_3:6067788-6067807 None:intergenic 40.0%
GGGGAAAAAATTTCACCTCT+CGG - chr4_3:6067789-6067808 None:intergenic 40.0%
CAAAAGAACCAACTGCGAAA+AGG + chr4_3:6066906-6066925 Msa0626480:CDS 40.0%
!!! TTTGCTCTGTTTTCTGAAGC+TGG + chr4_3:6067993-6068012 Msa0626480:intron 40.0%
AGTTAACAGATGAGGAGTAG+TGG + chr4_3:6068113-6068132 Msa0626480:CDS 40.0%
!! CTATTGAACCGGCTTGATTT+AGG + chr4_3:6068168-6068187 Msa0626480:three_prime_UTR 40.0%
! AGCTTTAGCCTAAATCAAGC+CGG - chr4_3:6068179-6068198 None:intergenic 40.0%
! ATATCTTTTCGCACTGTCCT+TGG + chr4_3:6068261-6068280 Msa0626480:three_prime_UTR 40.0%
CAGGCATTAGCAATATTCCA+AGG - chr4_3:6068281-6068300 None:intergenic 40.0%
ATGATCGCAGGATATAGAAG+AGG - chr4_3:6068339-6068358 None:intergenic 40.0%
TAACTGAGGGATCTGTTAGA+TGG + chr4_3:6068443-6068462 Msa0626480:three_prime_UTR 40.0%
AACTGAGGGATCTGTTAGAT+GGG + chr4_3:6068444-6068463 Msa0626480:three_prime_UTR 40.0%
TCCTACTTAACCAGATGCTT+TGG + chr4_3:6068499-6068518 Msa0626480:three_prime_UTR 40.0%
! ACCAAAGCATCTGGTTAAGT+AGG - chr4_3:6068503-6068522 None:intergenic 40.0%
CAAAATAGACCATCAGGGAT+AGG - chr4_3:6068652-6068671 None:intergenic 40.0%
CTGGTTAAGGAAGGCAAAAG+AGG + chr4_3:6065996-6066015 Msa0626480:intron 45.0%
AAATGTTAACCAGTGTCCCC+GGG - chr4_3:6066119-6066138 None:intergenic 45.0%
GAAATGTTAACCAGTGTCCC+CGG - chr4_3:6066120-6066139 None:intergenic 45.0%
ACAATATTCAGCCAGGGCTA+CGG + chr4_3:6066276-6066295 Msa0626480:five_prime_UTR 45.0%
CTGAAGTGAAGAAACCTGAG+AGG + chr4_3:6066784-6066803 Msa0626480:CDS 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - chr4_3:6066917-6066936 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + chr4_3:6066917-6066936 Msa0626480:CDS 45.0%
CTGCGAAAAGGGCTAAAGTT+GGG + chr4_3:6066918-6066937 Msa0626480:CDS 45.0%
GGAGAAGAACGAACCATTTG+GGG - chr4_3:6067293-6067312 None:intergenic 45.0%
! TCTGGTGTCAGTAGAAGAGA+AGG - chr4_3:6067314-6067333 None:intergenic 45.0%
!! TGTAATTGTAAGTGTCGGGG+CGG + chr4_3:6067395-6067414 Msa0626480:intron 45.0%
!! GAGAAGTGTCCATGCTTCAT+AGG + chr4_3:6067426-6067445 Msa0626480:intron 45.0%
GATCAGAGTCCTATGAAGCA+TGG - chr4_3:6067438-6067457 None:intergenic 45.0%
CTACAATTGTGCGTGTCAAC+GGG - chr4_3:6067600-6067619 None:intergenic 45.0%
GCTACAATTGTGCGTGTCAA+CGG - chr4_3:6067601-6067620 None:intergenic 45.0%
! ACTTTAGCCCTTTTCGCAGT+TGG - chr4_3:6066917-6066936 None:intergenic 45.0%
ACTGCGAAAAGGGCTAAAGT+TGG + chr4_3:6066917-6066936 Msa0626480:CDS 45.0%
GGATCAGGAGGGATATGATA+AGG + chr4_3:6068033-6068052 Msa0626480:intron 45.0%
GGAAGGCCAATCTGATGATA+AGG + chr4_3:6068063-6068082 Msa0626480:CDS 45.0%
GGGCTTCCTTATCATCAGAT+TGG - chr4_3:6068072-6068091 None:intergenic 45.0%
GGTGGAAGAGTTAACAGATG+AGG + chr4_3:6068105-6068124 Msa0626480:CDS 45.0%
! GAGGAGTAGTGGTAGCATTT+TGG + chr4_3:6068124-6068143 Msa0626480:exon 45.0%
GGCAAGTTTAACATGATCGC+AGG - chr4_3:6068351-6068370 None:intergenic 45.0%
TGGGCAATGACTTCTGTGAA+TGG + chr4_3:6068463-6068482 Msa0626480:three_prime_UTR 45.0%
ACGATTGATCCTATCCCTGA+TGG + chr4_3:6068640-6068659 Msa0626480:three_prime_UTR 45.0%
AATGTTAACCAGTGTCCCCG+GGG - chr4_3:6066118-6066137 None:intergenic 50.0%
GTAGCCCTGGCTGAATATTG+TGG - chr4_3:6066277-6066296 None:intergenic 50.0%
!! CAATGAAAGTACCGTAGCCC+TGG - chr4_3:6066290-6066309 None:intergenic 50.0%
! ATATTCGGACGAGGCACTCT+TGG + chr4_3:6066641-6066660 Msa0626480:CDS 50.0%
ATGGCAGTGCTTTCCCCAAA+TGG + chr4_3:6067277-6067296 Msa0626480:CDS 50.0%
TACTGACACCAGACACGACA+TGG + chr4_3:6067321-6067340 Msa0626480:intron 50.0%
GATTGTGTCCATGTCGTGTC+TGG - chr4_3:6067332-6067351 None:intergenic 50.0%
GCATGGACACTTCTCGGATT+AGG - chr4_3:6067421-6067440 None:intergenic 50.0%
CTGAAGTGAAGAAACCCGAG+AGG + chr4_3:6067771-6067790 Msa0626480:CDS 50.0%
GTTAACTCTTCCACCTCTCC+TGG - chr4_3:6068100-6068119 None:intergenic 50.0%
ATTCGCTGCGCTCACTAGTT+CGG + chr4_3:6068676-6068695 Msa0626480:three_prime_UTR 50.0%
GTCATGTTAACCAGTGCCCC+CGG + chr4_3:6065969-6065988 Msa0626480:intron 55.0%
TCATGTTAACCAGTGCCCCC+GGG + chr4_3:6065970-6065989 Msa0626480:intron 55.0%
TTGACCCTTAACCAGTGCCC+CGG + chr4_3:6066098-6066117 Msa0626480:intron 55.0%
CAACTCCAAGTCCATGGTGG+CGG - chr4_3:6066580-6066599 None:intergenic 55.0%
CTTCGGCAACTCCAAGTCCA+TGG - chr4_3:6066586-6066605 None:intergenic 55.0%
CATGGACTTGGAGTTGCCGA+AGG + chr4_3:6066584-6066603 Msa0626480:exon 55.0%
CATGTTAACCAGTGCCCCCG+GGG + chr4_3:6065971-6065990 Msa0626480:intron 60.0%
TTCCTTAACCAGTGCCCCGG+GGG - chr4_3:6065988-6066007 None:intergenic 60.0%
CTTCCTTAACCAGTGCCCCG+GGG - chr4_3:6065989-6066008 None:intergenic 60.0%
CCTTCCTTAACCAGTGCCCC+GGG - chr4_3:6065990-6066009 None:intergenic 60.0%
GCCTTCCTTAACCAGTGCCC+CGG - chr4_3:6065991-6066010 None:intergenic 60.0%
TGACCCTTAACCAGTGCCCC+GGG + chr4_3:6066099-6066118 Msa0626480:intron 60.0%
AGAAACCCTTGCCGCCACCA+TGG + chr4_3:6066566-6066585 Msa0626480:intron 60.0%
!! CAAGTCCATGGTGGCGGCAA+GGG - chr4_3:6066574-6066593 None:intergenic 60.0%
CGGCAACTCCAAGTCCATGG+TGG - chr4_3:6066583-6066602 None:intergenic 60.0%
TGCGTGTCAACGGGTGCAAC+AGG - chr4_3:6067591-6067610 None:intergenic 60.0%
GATAAGGAAGCCCCTGCACC+AGG + chr4_3:6068079-6068098 Msa0626480:CDS 60.0%
TTCCACCTCTCCTGGTGCAG+GGG - chr4_3:6068092-6068111 None:intergenic 60.0%
CTTCCACCTCTCCTGGTGCA+GGG - chr4_3:6068093-6068112 None:intergenic 60.0%
TCTTCCACCTCTCCTGGTGC+AGG - chr4_3:6068094-6068113 None:intergenic 60.0%
!! CCCGGGGCACTGGTTAAGGA+AGG + chr4_3:6065987-6066006 Msa0626480:intron 65.0%
GACCCTTAACCAGTGCCCCG+GGG + chr4_3:6066100-6066119 Msa0626480:intron 65.0%
!! GTCCCCGGGGCACTGGTTAA+GGG - chr4_3:6066105-6066124 None:intergenic 65.0%
!! TGTCCCCGGGGCACTGGTTA+AGG - chr4_3:6066106-6066125 None:intergenic 65.0%
!! CCAAGTCCATGGTGGCGGCA+AGG - chr4_3:6066575-6066594 None:intergenic 65.0%
CCTTGCCGCCACCATGGACT+TGG + chr4_3:6066572-6066591 Msa0626480:exon 65.0%
!! TGCCCCCGGGGCACTGGTTA+AGG + chr4_3:6065983-6066002 Msa0626480:intron 70.0%
AACCAGTGCCCCGGGGACAC+TGG + chr4_3:6066107-6066126 Msa0626480:intron 70.0%
AACCAGTGTCCCCGGGGCAC+TGG - chr4_3:6066112-6066131 None:intergenic 70.0%
GGAAGCCCCTGCACCAGGAG+AGG + chr4_3:6068084-6068103 Msa0626480:CDS 70.0%
AGCCCCTGCACCAGGAGAGG+TGG + chr4_3:6068087-6068106 Msa0626480:CDS 70.0%
AACCAGTGCCCCCGGGGCAC+TGG + chr4_3:6065977-6065996 Msa0626480:intron 75.0%
! AACCAGTGCCCCGGGGGCAC+TGG - chr4_3:6065982-6066001 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 6065901 6068840 6065901 ID=Msa0626480;Name=Msa0626480
chr4_3 mRNA 6065901 6068840 6065901 ID=Msa0626480-mRNA-1;Parent=Msa0626480;Name=Msa0626480-mRNA-1;_AED=0.01;_eAED=0.01;_QI=230|0.87|0.88|1|0.75|0.66|9|708|257
chr4_3 exon 6065901 6065962 6065901 ID=Msa0626480-mRNA-1:exon:1699;Parent=Msa0626480-mRNA-1
chr4_3 exon 6066147 6066297 6066147 ID=Msa0626480-mRNA-1:exon:1700;Parent=Msa0626480-mRNA-1
chr4_3 exon 6066568 6066805 6066568 ID=Msa0626480-mRNA-1:exon:1701;Parent=Msa0626480-mRNA-1
chr4_3 exon 6066892 6066979 6066892 ID=Msa0626480-mRNA-1:exon:1702;Parent=Msa0626480-mRNA-1
chr4_3 exon 6067102 6067298 6067102 ID=Msa0626480-mRNA-1:exon:1703;Parent=Msa0626480-mRNA-1
chr4_3 exon 6067566 6067639 6067566 ID=Msa0626480-mRNA-1:exon:1704;Parent=Msa0626480-mRNA-1
chr4_3 exon 6067747 6067792 6067747 ID=Msa0626480-mRNA-1:exon:1705;Parent=Msa0626480-mRNA-1
chr4_3 exon 6067889 6067937 6067889 ID=Msa0626480-mRNA-1:exon:1706;Parent=Msa0626480-mRNA-1
chr4_3 exon 6068034 6068840 6068034 ID=Msa0626480-mRNA-1:exon:1707;Parent=Msa0626480-mRNA-1
chr4_3 five_prime_UTR 6065901 6065962 6065901 ID=Msa0626480-mRNA-1:five_prime_utr;Parent=Msa0626480-mRNA-1
chr4_3 five_prime_UTR 6066147 6066297 6066147 ID=Msa0626480-mRNA-1:five_prime_utr;Parent=Msa0626480-mRNA-1
chr4_3 five_prime_UTR 6066568 6066584 6066568 ID=Msa0626480-mRNA-1:five_prime_utr;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6066585 6066805 6066585 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6066892 6066979 6066892 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6067102 6067298 6067102 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6067566 6067639 6067566 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6067747 6067792 6067747 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6067889 6067937 6067889 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 CDS 6068034 6068132 6068034 ID=Msa0626480-mRNA-1:cds;Parent=Msa0626480-mRNA-1
chr4_3 three_prime_UTR 6068133 6068840 6068133 ID=Msa0626480-mRNA-1:three_prime_utr;Parent=Msa0626480-mRNA-1
Gene Sequence

>Msa0626480

ATGGACTTGGAGTTGCCGAAGGATCATCCATTATACGGTTGTCGCTTTCTAAATTTATATTCGGACGAGGCACTCTTGGAGTTATATAAGTACGAAGATAAGGTGGACGCTAAATTTGATCTGTGTCTTTTTGAAGATCTTCGCGAAGCTAAATTGGAAAAAGATGAACAAAATCTTTGTTTAGATTCTGAGCAAGCTTCTGAAGTGAAGAAACCTGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGGAAAGGGAAAAATGTTAATGAATCTAATGCTGAAAATGAACAGCAGGAGGCTTGTGCAGTATACCATGTTTTTGAAGAAATGATTCAGTTACCTGCAGCAAAGAAGATTTGTGATGAGCATAATGTTGATGAAAAAATTCTCACAAAAGTCTTGGAGAATTTGAAGAATTCCATTACCCAAGAAGCTTTGATTCAACTAGAAATAAGTGACAAAGATGGCAGTGCTTTCCCCAAATGTGATGAGAGTAAGAAAGATGTTCCTGTTGCACCCGTTGACACGCACAATTGTAGCCTTGAAGCCAAAATCAAAGATTCTCATGCTGTTGAGCAAGCTGCTGAAGTGAAGAAACCCGAGAGGAAGAAACCAAATTCAAAAGAACCAACTGCGAAAAGGGCTAAAGTTGGAGATCAGGAGGGATATGATAAGGAAGATAAGGAAGGCCAATCTGATGATAAGGAAGCCCCTGCACCAGGAGAGGTGGAAGAGTTAACAGATGAGGAGTAG
Protein sequence

>Msa0626480

MDLELPKDHPLYGCRFLNLYSDEALLELYKYEDKVDAKFDLCLFEDLREAKLEKDEQNLCLDSEQASEVKKPERKKPNSKEPTAKRAKVGKGKNVNESNAENEQQEACAVYHVFEEMIQLPAAKKICDEHNVDEKILTKVLENLKNSITQEALIQLEISDKDGSAFPKCDESKKDVPVAPVDTHNCSLEAKIKDSHAVEQAAEVKKPERKKPNSKEPTAKRAKVGDQEGYDKEDKEGQSDDKEAPAPGEVEELTDEE*