AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0627030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627030 MtrunA17_Chr4g0003201 96.809 282 7 1 1 282 1 280 0.0 560
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627030 AT3G15820.1 77.830 212 47 0 49 260 69 280 6.81e-123 353
Msa0627030 AT3G15830.1 69.528 233 70 1 49 281 64 295 3.83e-117 338

Find 93 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAACGAGTTATATATTA+TGG 0.202134 4_3:+7120104 Msa0627030:CDS
TTCTTCTCCACCCTTTGATT+TGG 0.205170 4_3:+7119218 Msa0627030:CDS
ACTACTACTGTTCATTGTTT+TGG 0.215489 4_3:-7118795 None:intergenic
TGTGGTTTGAATATATTTCT+AGG 0.233839 4_3:-7121815 None:intergenic
AAAGGGAAACACACAGAAAA+AGG 0.257363 4_3:+7121461 Msa0627030:three_prime_UTR
TCTTCTCCACCCTTTGATTT+GGG 0.276299 4_3:+7119219 Msa0627030:CDS
GTTAGAGTGATTGGTTTGAT+TGG 0.280580 4_3:-7119004 None:intergenic
TGTGGAATACACGCTCTTTA+TGG 0.285794 4_3:+7119188 Msa0627030:CDS
TTCAAACTCCTCCTCATGTT+TGG 0.314633 4_3:-7121626 None:intergenic
GGAATGTAATGAAGGCATTA+GGG 0.316804 4_3:-7118901 None:intergenic
TGGAATGTAATGAAGGCATT+AGG 0.332816 4_3:-7118902 None:intergenic
TGCAACCAAATCATGTTTAG+AGG 0.340593 4_3:-7121415 None:intergenic
TTAGAGTGATTGGTTTGATT+GGG 0.342876 4_3:-7119003 None:intergenic
CTTCAGCTTCGTTCATGAAA+TGG 0.346389 4_3:+7119085 Msa0627030:CDS
AGGCCATTACACTATTGATT+TGG 0.346538 4_3:+7121293 Msa0627030:CDS
TACACTATTGATTTGGCTGT+TGG 0.354110 4_3:+7121300 Msa0627030:CDS
TTCGGGTCATGTGGCGGGTT+CGG 0.364904 4_3:+7121170 Msa0627030:CDS
ATTGATTTGGCTGTTGGGTT+AGG 0.379250 4_3:+7121306 Msa0627030:CDS
CCATGCCTCTAAACATGATT+TGG 0.379481 4_3:+7121410 Msa0627030:CDS
CGAGTTATATATTATGGACT+TGG 0.380434 4_3:+7120110 Msa0627030:CDS
GAAGAAGAATTCTTCGGCAT+TGG 0.403432 4_3:-7119036 None:intergenic
TTGGCTGTTGGGTTAGGTGC+TGG 0.405118 4_3:+7121312 Msa0627030:CDS
GGGTGGTGTGTCTGCGTTTG+AGG 0.411025 4_3:-7118981 None:intergenic
TATATATTTGACTCTTTAGC+TGG 0.413885 4_3:+7121336 Msa0627030:CDS
AGAGGCGGCGCCGCCGTTCA+TGG 0.424186 4_3:-7118938 None:intergenic
ACATGAGGAGGATGAAGAGA+TGG 0.426409 4_3:+7121208 Msa0627030:CDS
TCACCTGAGGCAATGGTAGC+TGG 0.428409 4_3:-7120210 None:intergenic
ACACTATTGATTTGGCTGTT+GGG 0.428713 4_3:+7121301 Msa0627030:CDS
GGTGGCGTCACCGGAGGAAG+AGG 0.434415 4_3:-7118956 None:intergenic
TACACGTGGCGACACATCAT+TGG 0.439823 4_3:+7119124 Msa0627030:CDS
CTGGTGTGGACTTTCCAGTT+GGG 0.441942 4_3:+7121124 Msa0627030:CDS
TTGCAAGCTGTGAGGTTACT+TGG 0.448382 4_3:+7121264 Msa0627030:CDS
TAGAGTGATTGGTTTGATTG+GGG 0.454594 4_3:-7119002 None:intergenic
GGCGGCGCCGCCTCTTCCTC+CGG 0.456995 4_3:+7118946 Msa0627030:CDS
TTTACATGTCGTGGGATTTG+TGG 0.464311 4_3:+7120178 Msa0627030:CDS
TTTGAGGACGGTGGCGTCAC+CGG 0.474389 4_3:-7118965 None:intergenic
CATGAGGAGGATGAAGAGAT+GGG 0.476682 4_3:+7121209 Msa0627030:CDS
TCTGGTGTGGACTTTCCAGT+TGG 0.481025 4_3:+7121123 Msa0627030:CDS
AGAGTGATTGGTTTGATTGG+GGG 0.483457 4_3:-7119001 None:intergenic
TTATGGACTTGGTTAATTGA+AGG 0.497483 4_3:+7120121 Msa0627030:CDS
AATTTGAGACTGTACCCAAA+AGG 0.497856 4_3:+7121443 Msa0627030:three_prime_UTR
TGTTCTAGCTGCTCTTAATA+CGG 0.502013 4_3:+7119299 Msa0627030:CDS
ATAAGATTCTGGCGAAATTG+AGG 0.502373 4_3:-7118869 None:intergenic
GCGCCGCCGTTCATGGTGGT+TGG 0.505272 4_3:-7118931 None:intergenic
GAAATTGAGGTTGTAATTGA+CGG 0.508751 4_3:-7118856 None:intergenic
CACCCAGCTACCATTGCCTC+AGG 0.509498 4_3:+7120207 Msa0627030:CDS
TTACATGTCGTGGGATTTGT+GGG 0.512699 4_3:+7120179 Msa0627030:CDS
CGACAAAATCATCTCCCATC+CGG 0.513619 4_3:-7121521 None:intergenic
GAGGATGAAGAGATGGGAGA+TGG 0.513956 4_3:+7121215 Msa0627030:CDS
ATTTGAGACTGTACCCAAAA+GGG 0.515747 4_3:+7121444 Msa0627030:three_prime_UTR
GAAAACAAAACCCAAATCAA+AGG 0.521787 4_3:-7119229 None:intergenic
GAATGAATCACCTGAGGCAA+TGG 0.526312 4_3:-7120217 None:intergenic
CAGCCAAATCAATAGTGTAA+TGG 0.530095 4_3:-7121296 None:intergenic
TGAAGAGATGGGAGATGGCA+TGG 0.534871 4_3:+7121220 Msa0627030:CDS
CACCTGAGGCAATGGTAGCT+GGG 0.539500 4_3:-7120209 None:intergenic
TTCATGGTGGTTGGAGAATG+TGG 0.541925 4_3:-7118922 None:intergenic
CCAAATCATGTTTAGAGGCA+TGG 0.542552 4_3:-7121410 None:intergenic
TTGTTGTAGCTCTAAAGTGT+AGG 0.545152 4_3:+7121555 Msa0627030:three_prime_UTR
GGTGTGTCTGCGTTTGAGGA+CGG 0.546169 4_3:-7118977 None:intergenic
AAAACAAAACCCAAATCAAA+GGG 0.546521 4_3:-7119228 None:intergenic
GAGAATGTGGAATGTAATGA+AGG 0.552700 4_3:-7118909 None:intergenic
AGACCACAAGCAAACAAACA+CGG 0.559564 4_3:-7119150 None:intergenic
TCATGTTTGGATTCTAACAT+CGG 0.577627 4_3:-7121613 None:intergenic
CATCAACATCATAATAACTT+CGG 0.578990 4_3:+7118828 Msa0627030:five_prime_UTR
CATTCTCCAACCACCATGAA+CGG 0.579463 4_3:+7118925 Msa0627030:exon
GGCGTCACCGGAGGAAGAGG+CGG 0.580308 4_3:-7118953 None:intergenic
ATGCCGTGTTTGTTTGCTTG+TGG 0.580559 4_3:+7119147 Msa0627030:CDS
GTGTATGTGTGGATGCCGGA+TGG 0.581183 4_3:+7121506 Msa0627030:three_prime_UTR
TCAAAGGGTGGAGAAGAAGA+AGG 0.587930 4_3:-7119213 None:intergenic
AAGACCTAGAGCAACAATCT+CGG 0.589198 4_3:+7120144 Msa0627030:CDS
AGAGCCGAGATTGTTGCTCT+AGG 0.591521 4_3:-7120148 None:intergenic
ATGTGTGTATGTGTGGATGC+CGG 0.594876 4_3:+7121502 Msa0627030:three_prime_UTR
ATGTAATGAAGGCATTAGGG+GGG 0.596763 4_3:-7118898 None:intergenic
TGTATGTGTGGATGCCGGAT+GGG 0.607384 4_3:+7121507 Msa0627030:three_prime_UTR
GTGAGGTTACTTGGTACAAG+AGG 0.610779 4_3:+7121273 Msa0627030:CDS
TTAGAGCTACAACAAAATCA+CGG 0.613938 4_3:-7121547 None:intergenic
GAGGACGGTGGCGTCACCGG+AGG 0.618518 4_3:-7118962 None:intergenic
GTAGGCAATGTGTGTATGTG+TGG 0.619686 4_3:+7121495 Msa0627030:three_prime_UTR
GAATGTAATGAAGGCATTAG+GGG 0.620843 4_3:-7118900 None:intergenic
TGATAGCTTCATTAGACATG+AGG 0.643958 4_3:+7121193 Msa0627030:CDS
TAGCTTCATTAGACATGAGG+AGG 0.645843 4_3:+7121196 Msa0627030:CDS
GCTAGAACATTATTGAGCTG+AGG 0.646448 4_3:-7119285 None:intergenic
ACAAAACCCAAATCAAAGGG+TGG 0.648382 4_3:-7119225 None:intergenic
GATGTTAGAATCCAAACATG+AGG 0.660469 4_3:+7121615 Msa0627030:three_prime_UTR
GTTAGAATCCAAACATGAGG+AGG 0.660606 4_3:+7121618 Msa0627030:three_prime_UTR
TGTAATGAAGGCATTAGGGG+GGG 0.665450 4_3:-7118897 None:intergenic
AATGTAATGAAGGCATTAGG+GGG 0.668257 4_3:-7118899 None:intergenic
GGCGGCGCCGCCGTTCATGG+TGG 0.676201 4_3:-7118935 None:intergenic
CTGAGGCAATGGTAGCTGGG+TGG 0.676709 4_3:-7120206 None:intergenic
TCTCCAACCACCATGAACGG+CGG 0.680353 4_3:+7118928 Msa0627030:exon
AATCTCAGATGTTGTACACG+TGG 0.689946 4_3:+7119110 Msa0627030:CDS
GTGTCTGCGTTTGAGGACGG+TGG 0.706981 4_3:-7118974 None:intergenic
TGAATGCATTGCAAGCTGTG+AGG 0.766804 4_3:+7121256 Msa0627030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTATTTTTTTTTGTT+TGG + chr4_3:7119532-7119551 Msa0627030:intron 10.0%
!!! TTTTTTTTGTTTGTTTCTAA+TGG + chr4_3:7119614-7119633 Msa0627030:intron 15.0%
!!! TCTGAATTGATTTTTTTTTT+TGG + chr4_3:7119642-7119661 Msa0627030:intron 15.0%
!!! AAAATACACCAAATTTTTTT+TGG - chr4_3:7119704-7119723 None:intergenic 15.0%
!!! AAAAAAAATTTGGTGTATTT+TGG + chr4_3:7119703-7119722 Msa0627030:intron 15.0%
!!! TTTATCTGAATTGATTTTTT+TGG + chr4_3:7120000-7120019 Msa0627030:intron 15.0%
!!! TTTTTTTTTGTTTGGCAATT+AGG + chr4_3:7119540-7119559 Msa0627030:intron 20.0%
!! TTTCTCTTCCAAAAAAAATT+TGG + chr4_3:7119693-7119712 Msa0627030:intron 20.0%
!! AGATACATTCATATAAAAGT+GGG - chr4_3:7119944-7119963 None:intergenic 20.0%
!! TAGATACATTCATATAAAAG+TGG - chr4_3:7119945-7119964 None:intergenic 20.0%
!! TTTAACATGAAATTGAAGTT+TGG + chr4_3:7120856-7120875 Msa0627030:intron 20.0%
!! CAAGTTAAAAATCACATTTA+GGG - chr4_3:7120890-7120909 None:intergenic 20.0%
!! TCAAGTTAAAAATCACATTT+AGG - chr4_3:7120891-7120910 None:intergenic 20.0%
!! ATTTGAATTCAATTAACATC+TGG + chr4_3:7120972-7120991 Msa0627030:intron 20.0%
!! TTTGAATTCAATTAACATCT+GGG + chr4_3:7120973-7120992 Msa0627030:intron 20.0%
!! CAAAACAAAATAAAAACACA+AGG - chr4_3:7121080-7121099 None:intergenic 20.0%
!!! ATCATTTTTCCTGTTTTTTT+CGG + chr4_3:7121152-7121171 Msa0627030:CDS 20.0%
! TGGATAAAAGACAGAAAAAT+TGG - chr4_3:7118722-7118741 None:intergenic 25.0%
!! GAAGAATTCTTTTCTTTTTG+TGG - chr4_3:7118742-7118761 None:intergenic 25.0%
! CATCAACATCATAATAACTT+CGG + chr4_3:7118828-7118847 Msa0627030:five_prime_UTR 25.0%
!!! ATTTTTTTGGTTAGAGTGAT+TGG - chr4_3:7119016-7119035 None:intergenic 25.0%
! AAAACAAAACCCAAATCAAA+GGG - chr4_3:7119231-7119250 None:intergenic 25.0%
! ATCAATCAAACACATTACAT+CGG - chr4_3:7119431-7119450 None:intergenic 25.0%
! TGTTTGATTGATATGCAAAT+GGG + chr4_3:7119439-7119458 Msa0627030:intron 25.0%
!! GTCAAAATTTGTGTTTTCTT+AGG + chr4_3:7119502-7119521 Msa0627030:intron 25.0%
!! AATTTGTGTTTTCTTAGGTT+TGG + chr4_3:7119507-7119526 Msa0627030:intron 25.0%
!! GCATTTTCTGAGTTAAAATT+TGG + chr4_3:7119664-7119683 Msa0627030:intron 25.0%
! GATATTATTAGATGTTCTCA+AGG + chr4_3:7119775-7119794 Msa0627030:intron 25.0%
!!! GGAAAATTGATTTTGTTTCT+TGG + chr4_3:7119796-7119815 Msa0627030:intron 25.0%
!!! AAACTCTAAATTCTAGCTTT+AGG - chr4_3:7119833-7119852 None:intergenic 25.0%
!!! ACGATAATATGTGTTTTGTT+TGG + chr4_3:7119966-7119985 Msa0627030:intron 25.0%
! TGCAAACGAGTTATATATTA+TGG + chr4_3:7120104-7120123 Msa0627030:CDS 25.0%
!!! TAACAATGAAGCATTTTGTT+TGG - chr4_3:7120272-7120291 None:intergenic 25.0%
!!! AAACATGCATTTAGTTCTAT+TGG - chr4_3:7120406-7120425 None:intergenic 25.0%
!! GAATTCAAACATAGTTCTTT+TGG - chr4_3:7120476-7120495 None:intergenic 25.0%
!! GAATTCAAACATAGTTCTTT+TGG - chr4_3:7120476-7120495 None:intergenic 25.0%
! TTTAACATGAAGTTGAAGTT+TGG + chr4_3:7120580-7120599 Msa0627030:intron 25.0%
!!! TAAATAGTCTCTGAAATTAG+TGG - chr4_3:7120942-7120961 None:intergenic 25.0%
!!! ATTTTGTTTTGATGTTGAAC+AGG + chr4_3:7121089-7121108 Msa0627030:intron 25.0%
!!! TTTTGTTTTGATGTTGAACA+GGG + chr4_3:7121090-7121109 Msa0627030:intron 25.0%
!!! TCATTTTTCCTGTTTTTTTC+GGG + chr4_3:7121153-7121172 Msa0627030:CDS 25.0%
! TATATATTTGACTCTTTAGC+TGG + chr4_3:7121336-7121355 Msa0627030:CDS 25.0%
! ATAGCTAAAAATAGTGTCAA+TGG + chr4_3:7121387-7121406 Msa0627030:CDS 25.0%
! AAAAAGGATAATAGAGAAGT+AGG + chr4_3:7121477-7121496 Msa0627030:three_prime_UTR 25.0%
! TGTGGTTTGAATATATTTCT+AGG - chr4_3:7121818-7121837 None:intergenic 25.0%
!!! CTGTTCATTGTTTTGGAAAA+GGG - chr4_3:7118791-7118810 None:intergenic 30.0%
!!! ACTGTTCATTGTTTTGGAAA+AGG - chr4_3:7118792-7118811 None:intergenic 30.0%
! ACTACTACTGTTCATTGTTT+TGG - chr4_3:7118798-7118817 None:intergenic 30.0%
GAAATTGAGGTTGTAATTGA+CGG - chr4_3:7118859-7118878 None:intergenic 30.0%
!! TTAGAGTGATTGGTTTGATT+GGG - chr4_3:7119006-7119025 None:intergenic 30.0%
GAAGAAGAAGAAGAATTCTT+CGG - chr4_3:7119045-7119064 None:intergenic 30.0%
GAAAACAAAACCCAAATCAA+AGG - chr4_3:7119232-7119251 None:intergenic 30.0%
! ATCTCGTTTATCTCTCTTTT+TGG + chr4_3:7119323-7119342 Msa0627030:intron 30.0%
GTGTTTGATTGATATGCAAA+TGG + chr4_3:7119438-7119457 Msa0627030:intron 30.0%
TTTGTTTGGCAATTAGGAAA+TGG + chr4_3:7119546-7119565 Msa0627030:intron 30.0%
TGTTTGCATGACATGAAAAT+GGG + chr4_3:7119573-7119592 Msa0627030:intron 30.0%
!! AATTTAGAGTTTTTGCCTCT+GGG + chr4_3:7119841-7119860 Msa0627030:intron 30.0%
! TGGGTTGAACAATAAACTAA+AGG - chr4_3:7119925-7119944 None:intergenic 30.0%
!!! GTTTTGTTTGCATTGTTAGT+TGG + chr4_3:7120042-7120061 Msa0627030:intron 30.0%
CGAGTTATATATTATGGACT+TGG + chr4_3:7120110-7120129 Msa0627030:CDS 30.0%
! TTATGGACTTGGTTAATTGA+AGG + chr4_3:7120121-7120140 Msa0627030:CDS 30.0%
! TTTTCATGTTTACATGTCGT+GGG + chr4_3:7120170-7120189 Msa0627030:CDS 30.0%
! ATTAGTCCTAAGAGCAATTT+TGG + chr4_3:7120363-7120382 Msa0627030:intron 30.0%
!! TGCATTTAGTTCTATTGGAT+TGG - chr4_3:7120401-7120420 None:intergenic 30.0%
! CAAACATAGTTCTTTTGGAT+TGG - chr4_3:7120471-7120490 None:intergenic 30.0%
CATTGTAAGCTTCTAGTAAT+GGG - chr4_3:7120523-7120542 None:intergenic 30.0%
TCATTGTAAGCTTCTAGTAA+TGG - chr4_3:7120524-7120543 None:intergenic 30.0%
TACTAGAAGCTTACAATGAA+AGG + chr4_3:7120525-7120544 Msa0627030:intron 30.0%
ACTAGAAGCTTACAATGAAA+GGG + chr4_3:7120526-7120545 Msa0627030:intron 30.0%
! CAAACATAGTTCTTTTGGAT+TGG - chr4_3:7120471-7120490 None:intergenic 30.0%
CATTGTAAGCTTCTAGTAAT+GGG - chr4_3:7120523-7120542 None:intergenic 30.0%
TCATTGTAAGCTTCTAGTAA+TGG - chr4_3:7120524-7120543 None:intergenic 30.0%
TACTAGAAGCTTACAATGAA+AGG + chr4_3:7120525-7120544 Msa0627030:intron 30.0%
ACTAGAAGCTTACAATGAAA+GGG + chr4_3:7120526-7120545 Msa0627030:intron 30.0%
! ATGAAGTTGAAGTTTGGAAT+TGG + chr4_3:7120820-7120839 Msa0627030:intron 30.0%
TTACAATTGCAAGATCAGAT+TGG + chr4_3:7121012-7121031 Msa0627030:intron 30.0%
AAAAATGATACATTCCCAAC+TGG - chr4_3:7121141-7121160 None:intergenic 30.0%
!!! TTTTCAACTTGCTATCTTCA+AGG - chr4_3:7121368-7121387 None:intergenic 30.0%
TTAGAGCTACAACAAAATCA+CGG - chr4_3:7121550-7121569 None:intergenic 30.0%
!! TGTAGGTTTTGTCAAAATTG+CGG + chr4_3:7121572-7121591 Msa0627030:three_prime_UTR 30.0%
TCATGTTTGGATTCTAACAT+CGG - chr4_3:7121616-7121635 None:intergenic 30.0%
ATAAGATTCTGGCGAAATTG+AGG - chr4_3:7118872-7118891 None:intergenic 35.0%
!! AATGTAATGAAGGCATTAGG+GGG - chr4_3:7118902-7118921 None:intergenic 35.0%
!! GAATGTAATGAAGGCATTAG+GGG - chr4_3:7118903-7118922 None:intergenic 35.0%
!! GGAATGTAATGAAGGCATTA+GGG - chr4_3:7118904-7118923 None:intergenic 35.0%
!! TGGAATGTAATGAAGGCATT+AGG - chr4_3:7118905-7118924 None:intergenic 35.0%
GAGAATGTGGAATGTAATGA+AGG - chr4_3:7118912-7118931 None:intergenic 35.0%
!! TAGAGTGATTGGTTTGATTG+GGG - chr4_3:7119005-7119024 None:intergenic 35.0%
!! GTTAGAGTGATTGGTTTGAT+TGG - chr4_3:7119007-7119026 None:intergenic 35.0%
!!! TCTTCGGCATTGGATTTTTT+TGG - chr4_3:7119029-7119048 None:intergenic 35.0%
! TTGTGGTCTTTTGTTCTTCA+TGG + chr4_3:7119164-7119183 Msa0627030:CDS 35.0%
! TGTTCTAGCTGCTCTTAATA+CGG + chr4_3:7119299-7119318 Msa0627030:CDS 35.0%
GTGTTTGCATGACATGAAAA+TGG + chr4_3:7119572-7119591 Msa0627030:intron 35.0%
!! GAATTTAGAGTTTTTGCCTC+TGG + chr4_3:7119840-7119859 Msa0627030:intron 35.0%
CATGTTATAATCAATCCCAG+AGG - chr4_3:7119859-7119878 None:intergenic 35.0%
! AAAATCTGTGTTTTGCGTGT+AGG + chr4_3:7120069-7120088 Msa0627030:intron 35.0%
! CTTTTCATGTTTACATGTCG+TGG + chr4_3:7120169-7120188 Msa0627030:CDS 35.0%
TGAAATGAATGAATCACCTG+AGG - chr4_3:7120226-7120245 None:intergenic 35.0%
TAACCTCCAAAATTGCTCTT+AGG - chr4_3:7120372-7120391 None:intergenic 35.0%
!!! CATAGTTCTTTTGGATTGGT+CGG - chr4_3:7120467-7120486 None:intergenic 35.0%
!!! CATAGTTCTTTTGGATTGGT+CGG - chr4_3:7120467-7120486 None:intergenic 35.0%
! TGATGTTGAACAGGGATTTT+TGG + chr4_3:7121098-7121117 Msa0627030:intron 35.0%
! GATGTTGAACAGGGATTTTT+GGG + chr4_3:7121099-7121118 Msa0627030:intron 35.0%
TGATAGCTTCATTAGACATG+AGG + chr4_3:7121193-7121212 Msa0627030:CDS 35.0%
! AGGCCATTACACTATTGATT+TGG + chr4_3:7121293-7121312 Msa0627030:CDS 35.0%
CAGCCAAATCAATAGTGTAA+TGG - chr4_3:7121299-7121318 None:intergenic 35.0%
!! TACACTATTGATTTGGCTGT+TGG + chr4_3:7121300-7121319 Msa0627030:CDS 35.0%
!! ACACTATTGATTTGGCTGTT+GGG + chr4_3:7121301-7121320 Msa0627030:CDS 35.0%
TGCAACCAAATCATGTTTAG+AGG - chr4_3:7121418-7121437 None:intergenic 35.0%
! AATTTGAGACTGTACCCAAA+AGG + chr4_3:7121443-7121462 Msa0627030:three_prime_UTR 35.0%
! ATTTGAGACTGTACCCAAAA+GGG + chr4_3:7121444-7121463 Msa0627030:three_prime_UTR 35.0%
! TTTCTGTGTGTTTCCCTTTT+GGG - chr4_3:7121460-7121479 None:intergenic 35.0%
! TTTTCTGTGTGTTTCCCTTT+TGG - chr4_3:7121461-7121480 None:intergenic 35.0%
AAAGGGAAACACACAGAAAA+AGG + chr4_3:7121461-7121480 Msa0627030:three_prime_UTR 35.0%
!! TTGTTGTAGCTCTAAAGTGT+AGG + chr4_3:7121555-7121574 Msa0627030:three_prime_UTR 35.0%
GATGTTAGAATCCAAACATG+AGG + chr4_3:7121615-7121634 Msa0627030:three_prime_UTR 35.0%
!! GGGGGGTATTTATAAGATTC+TGG - chr4_3:7118883-7118902 None:intergenic 40.0%
!! ATGTAATGAAGGCATTAGGG+GGG - chr4_3:7118901-7118920 None:intergenic 40.0%
!! AGAGTGATTGGTTTGATTGG+GGG - chr4_3:7119004-7119023 None:intergenic 40.0%
GAAGAAGAATTCTTCGGCAT+TGG - chr4_3:7119039-7119058 None:intergenic 40.0%
CTTCAGCTTCGTTCATGAAA+TGG + chr4_3:7119085-7119104 Msa0627030:CDS 40.0%
AATCTCAGATGTTGTACACG+TGG + chr4_3:7119110-7119129 Msa0627030:CDS 40.0%
AGACCACAAGCAAACAAACA+CGG - chr4_3:7119153-7119172 None:intergenic 40.0%
! TCTTTTGTTCTTCATGGCTG+TGG + chr4_3:7119170-7119189 Msa0627030:CDS 40.0%
TGTGGAATACACGCTCTTTA+TGG + chr4_3:7119188-7119207 Msa0627030:CDS 40.0%
TTCTTCTCCACCCTTTGATT+TGG + chr4_3:7119218-7119237 Msa0627030:CDS 40.0%
! TCTTCTCCACCCTTTGATTT+GGG + chr4_3:7119219-7119238 Msa0627030:CDS 40.0%
ACAAAACCCAAATCAAAGGG+TGG - chr4_3:7119228-7119247 None:intergenic 40.0%
GCTAGAACATTATTGAGCTG+AGG - chr4_3:7119288-7119307 None:intergenic 40.0%
AAGACCTAGAGCAACAATCT+CGG + chr4_3:7120144-7120163 Msa0627030:CDS 40.0%
TTTACATGTCGTGGGATTTG+TGG + chr4_3:7120178-7120197 Msa0627030:CDS 40.0%
TTACATGTCGTGGGATTTGT+GGG + chr4_3:7120179-7120198 Msa0627030:CDS 40.0%
! AGTCCTAAGAGCAATTTTGG+AGG + chr4_3:7120366-7120385 Msa0627030:intron 40.0%
!! GTCTCTGAAATTAGTGGCAT+AGG - chr4_3:7120936-7120955 None:intergenic 40.0%
TAGCTTCATTAGACATGAGG+AGG + chr4_3:7121196-7121215 Msa0627030:CDS 40.0%
!! ATTGATTTGGCTGTTGGGTT+AGG + chr4_3:7121306-7121325 Msa0627030:CDS 40.0%
CCAAATCATGTTTAGAGGCA+TGG - chr4_3:7121413-7121432 None:intergenic 40.0%
CCATGCCTCTAAACATGATT+TGG + chr4_3:7121410-7121429 Msa0627030:CDS 40.0%
GTTAGAATCCAAACATGAGG+AGG + chr4_3:7121618-7121637 Msa0627030:three_prime_UTR 40.0%
TTCAAACTCCTCCTCATGTT+TGG - chr4_3:7121629-7121648 None:intergenic 40.0%
!! TGTAATGAAGGCATTAGGGG+GGG - chr4_3:7118900-7118919 None:intergenic 45.0%
TTCATGGTGGTTGGAGAATG+TGG - chr4_3:7118925-7118944 None:intergenic 45.0%
CATTCTCCAACCACCATGAA+CGG + chr4_3:7118925-7118944 Msa0627030:exon 45.0%
ATGCCGTGTTTGTTTGCTTG+TGG + chr4_3:7119147-7119166 Msa0627030:CDS 45.0%
TCAAAGGGTGGAGAAGAAGA+AGG - chr4_3:7119216-7119235 None:intergenic 45.0%
GAATGAATCACCTGAGGCAA+TGG - chr4_3:7120220-7120239 None:intergenic 45.0%
!!! GAACAGGGATTTTTGGGTTC+TGG + chr4_3:7121105-7121124 Msa0627030:intron 45.0%
CCACATGACCCGAAAAAAAC+AGG - chr4_3:7121164-7121183 None:intergenic 45.0%
!!! CCTGTTTTTTTCGGGTCATG+TGG + chr4_3:7121161-7121180 Msa0627030:CDS 45.0%
!!! GTTTTTTTCGGGTCATGTGG+CGG + chr4_3:7121164-7121183 Msa0627030:CDS 45.0%
!!! TTTTTTTCGGGTCATGTGGC+GGG + chr4_3:7121165-7121184 Msa0627030:CDS 45.0%
ACATGAGGAGGATGAAGAGA+TGG + chr4_3:7121208-7121227 Msa0627030:CDS 45.0%
CATGAGGAGGATGAAGAGAT+GGG + chr4_3:7121209-7121228 Msa0627030:CDS 45.0%
TGAATGCATTGCAAGCTGTG+AGG + chr4_3:7121256-7121275 Msa0627030:CDS 45.0%
TTGCAAGCTGTGAGGTTACT+TGG + chr4_3:7121264-7121283 Msa0627030:CDS 45.0%
GTGAGGTTACTTGGTACAAG+AGG + chr4_3:7121273-7121292 Msa0627030:CDS 45.0%
GTAGGCAATGTGTGTATGTG+TGG + chr4_3:7121495-7121514 Msa0627030:three_prime_UTR 45.0%
ATGTGTGTATGTGTGGATGC+CGG + chr4_3:7121502-7121521 Msa0627030:three_prime_UTR 45.0%
CGACAAAATCATCTCCCATC+CGG - chr4_3:7121524-7121543 None:intergenic 45.0%
!!! ATTTTTTGATTTTTTTTTTA+TGG + chr4_3:7119397-7119416 Msa0627030:intron 5.0%
!!! TTTTTTGATTTTTTTTTTAT+GGG + chr4_3:7119398-7119417 Msa0627030:intron 5.0%
!! GTGATTGGTTTGATTGGGGG+TGG - chr4_3:7119001-7119020 None:intergenic 50.0%
TACACGTGGCGACACATCAT+TGG + chr4_3:7119124-7119143 Msa0627030:CDS 50.0%
AGAGCCGAGATTGTTGCTCT+AGG - chr4_3:7120151-7120170 None:intergenic 50.0%
!!! GGGATTTTTGGGTTCTGGTG+TGG + chr4_3:7121110-7121129 Msa0627030:intron 50.0%
TCTGGTGTGGACTTTCCAGT+TGG + chr4_3:7121123-7121142 Msa0627030:CDS 50.0%
CTGGTGTGGACTTTCCAGTT+GGG + chr4_3:7121124-7121143 Msa0627030:CDS 50.0%
GAGGATGAAGAGATGGGAGA+TGG + chr4_3:7121215-7121234 Msa0627030:CDS 50.0%
TGAAGAGATGGGAGATGGCA+TGG + chr4_3:7121220-7121239 Msa0627030:CDS 50.0%
TGTATGTGTGGATGCCGGAT+GGG + chr4_3:7121507-7121526 Msa0627030:three_prime_UTR 50.0%
TCTCCAACCACCATGAACGG+CGG + chr4_3:7118928-7118947 Msa0627030:exon 55.0%
GGTGTGTCTGCGTTTGAGGA+CGG - chr4_3:7118980-7118999 None:intergenic 55.0%
CACCTGAGGCAATGGTAGCT+GGG - chr4_3:7120212-7120231 None:intergenic 55.0%
TCACCTGAGGCAATGGTAGC+TGG - chr4_3:7120213-7120232 None:intergenic 55.0%
! TTGGCTGTTGGGTTAGGTGC+TGG + chr4_3:7121312-7121331 Msa0627030:CDS 55.0%
GTGTATGTGTGGATGCCGGA+TGG + chr4_3:7121506-7121525 Msa0627030:three_prime_UTR 55.0%
TTTGAGGACGGTGGCGTCAC+CGG - chr4_3:7118968-7118987 None:intergenic 60.0%
GTGTCTGCGTTTGAGGACGG+TGG - chr4_3:7118977-7118996 None:intergenic 60.0%
GGGTGGTGTGTCTGCGTTTG+AGG - chr4_3:7118984-7119003 None:intergenic 60.0%
CTGAGGCAATGGTAGCTGGG+TGG - chr4_3:7120209-7120228 None:intergenic 60.0%
CACCCAGCTACCATTGCCTC+AGG + chr4_3:7120207-7120226 Msa0627030:CDS 60.0%
!! TTCGGGTCATGTGGCGGGTT+CGG + chr4_3:7121170-7121189 Msa0627030:CDS 60.0%
GCGCCGCCGTTCATGGTGGT+TGG - chr4_3:7118934-7118953 None:intergenic 70.0%
GGCGTCACCGGAGGAAGAGG+CGG - chr4_3:7118956-7118975 None:intergenic 70.0%
GGTGGCGTCACCGGAGGAAG+AGG - chr4_3:7118959-7118978 None:intergenic 70.0%
AGAGGCGGCGCCGCCGTTCA+TGG - chr4_3:7118941-7118960 None:intergenic 75.0%
GAGGACGGTGGCGTCACCGG+AGG - chr4_3:7118965-7118984 None:intergenic 75.0%
! GGCGGCGCCGCCGTTCATGG+TGG - chr4_3:7118938-7118957 None:intergenic 80.0%
! GGCGGCGCCGCCTCTTCCTC+CGG + chr4_3:7118946-7118965 Msa0627030:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 7118719 7121850 7118719 ID=Msa0627030;Name=Msa0627030
chr4_3 mRNA 7118719 7121850 7118719 ID=Msa0627030-mRNA-1;Parent=Msa0627030;Name=Msa0627030-mRNA-1;_AED=0.30;_eAED=0.30;_QI=221|1|1|1|1|1|3|410|282
chr4_3 exon 7118719 7119320 7118719 ID=Msa0627030-mRNA-1:exon:2080;Parent=Msa0627030-mRNA-1
chr4_3 exon 7120091 7120228 7120091 ID=Msa0627030-mRNA-1:exon:2081;Parent=Msa0627030-mRNA-1
chr4_3 exon 7121111 7121850 7121111 ID=Msa0627030-mRNA-1:exon:2082;Parent=Msa0627030-mRNA-1
chr4_3 five_prime_UTR 7118719 7118939 7118719 ID=Msa0627030-mRNA-1:five_prime_utr;Parent=Msa0627030-mRNA-1
chr4_3 CDS 7118940 7119320 7118940 ID=Msa0627030-mRNA-1:cds;Parent=Msa0627030-mRNA-1
chr4_3 CDS 7120091 7120228 7120091 ID=Msa0627030-mRNA-1:cds;Parent=Msa0627030-mRNA-1
chr4_3 CDS 7121111 7121440 7121111 ID=Msa0627030-mRNA-1:cds;Parent=Msa0627030-mRNA-1
chr4_3 three_prime_UTR 7121441 7121850 7121441 ID=Msa0627030-mRNA-1:three_prime_utr;Parent=Msa0627030-mRNA-1
Gene Sequence

>Msa0627030

ATGAACGGCGGCGCCGCCTCTTCCTCCGGTGACGCCACCGTCCTCAAACGCAGACACACCACCCCCAATCAAACCAATCACTCTAACCAAAAAAATCCAATGCCGAAGAATTCTTCTTCTTCTTCTTCTCTTTGTTCTCTGTATTCTTCAGCTTCGTTCATGAAATGGACAATCTCAGATGTTGTACACGTGGCGACACATCATTGGATGCCGTGTTTGTTTGCTTGTGGTCTTTTGTTCTTCATGGCTGTGGAATACACGCTCTTTATGGTTCCTTCTTCTTCTCCACCCTTTGATTTGGGTTTTGTTTTCACTCGCTCTCTTCATCGTGTTCTTGAATCTTCTCCTCAGCTCAATAATGTTCTAGCTGCTCTTAATACGGTGTTTGTGTTAATGCAAACGAGTTATATATTATGGACTTGGTTAATTGAAGGAAGACCTAGAGCAACAATCTCGGCTCTTTTCATGTTTACATGTCGTGGGATTTGTGGGTATTCCACCCAGCTACCATTGCCTCAGGGATTTTTGGGTTCTGGTGTGGACTTTCCAGTTGGGAATGTATCATTTTTCCTGTTTTTTTCGGGTCATGTGGCGGGTTCGGTGATAGCTTCATTAGACATGAGGAGGATGAAGAGATGGGAGATGGCATGGACTTTTGATTTGTTGAATGCATTGCAAGCTGTGAGGTTACTTGGTACAAGAGGCCATTACACTATTGATTTGGCTGTTGGGTTAGGTGCTGGCTATATATTTGACTCTTTAGCTGGTAAGTACCTTGAAGATAGCAAGTTGAAAATAGCTAAAAATAGTGTCAATGGCCATGCCTCTAAACATGATTTGGTTGCATGA
Protein sequence

>Msa0627030

MNGGAASSSGDATVLKRRHTTPNQTNHSNQKNPMPKNSSSSSSLCSLYSSASFMKWTISDVVHVATHHWMPCLFACGLLFFMAVEYTLFMVPSSSPPFDLGFVFTRSLHRVLESSPQLNNVLAALNTVFVLMQTSYILWTWLIEGRPRATISALFMFTCRGICGYSTQLPLPQGFLGSGVDFPVGNVSFFLFFSGHVAGSVIASLDMRRMKRWEMAWTFDLLNALQAVRLLGTRGHYTIDLAVGLGAGYIFDSLAGKYLEDSKLKIAKNSVNGHASKHDLVA*