AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0634020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0634020 MtrunA17_Chr4g0008981 46.939 147 23 4 1 92 10 156 6.02e-32 108
Msa0634020 MtrunA17_Chr4g0009241 69.492 59 18 0 1 59 1446 1504 6.88e-24 94.0
Msa0634020 MtrunA17_Chr4g0009141 71.930 57 16 0 1 57 1442 1498 8.97e-23 90.9
Msa0634020 MtrunA17_Chr4g0009171 69.091 55 17 0 1 55 1647 1701 1.11e-20 84.7
Msa0634020 MtrunA17_Chr4g0009401 67.273 55 18 0 1 55 1424 1478 2.87e-20 83.6
Msa0634020 MtrunA17_Chr4g0009011 63.158 57 21 0 1 57 210 266 2.79e-19 80.1
Msa0634020 MtrunA17_Chr4g0009211 64.912 57 20 0 1 57 10 66 3.84e-19 76.3
Msa0634020 MtrunA17_Chr4g0009041 67.273 55 18 0 1 55 1430 1484 6.46e-19 79.7
Msa0634020 MtrunA17_Chr4g0009361 70.370 54 16 0 2 55 439 492 1.43e-18 78.6
Msa0634020 MtrunA17_Chr4g0009101 63.158 57 21 0 1 57 1458 1514 2.63e-18 78.2
Msa0634020 MtrunA17_Chr4g0009301 63.636 55 20 0 1 55 1462 1516 1.37e-17 75.9
Msa0634020 MtrunA17_Chr4g0005871 48.276 58 30 0 1 58 1055 1112 1.13e-14 67.8
Msa0634020 MtrunA17_Chr5g0414761 55.556 54 24 0 6 59 712 765 4.67e-14 65.9
Msa0634020 MtrunA17_Chr3g0085531 48.276 58 30 0 2 59 120 177 4.97e-14 65.5
Msa0634020 MtrunA17_Chr4g0005591 46.552 58 31 0 1 58 1007 1064 1.62e-13 64.3
Msa0634020 MtrunA17_Chr4g0005891 46.552 58 31 0 1 58 979 1036 1.91e-13 64.3
Msa0634020 MtrunA17_Chr3g0085731 52.830 53 25 0 2 54 930 982 2.54e-13 63.9
Msa0634020 MtrunA17_Chr8g0352211 52.830 53 25 0 2 54 852 904 2.82e-13 63.5
Msa0634020 MtrunA17_Chr4g0005851 44.828 58 32 0 1 58 965 1022 3.53e-13 63.5
Msa0634020 MtrunA17_Chr8g0370511 50.000 58 29 0 2 59 922 979 3.57e-13 63.5
Msa0634020 MtrunA17_Chr8g0339011 50.000 54 27 0 6 59 921 974 4.21e-13 63.2
Msa0634020 MtrunA17_Chr8g0338981 51.852 54 26 0 6 59 925 978 5.82e-13 62.8
Msa0634020 MtrunA17_Chr8g0338881 55.102 49 22 0 11 59 962 1010 7.16e-13 62.4
Msa0634020 MtrunA17_Chr8g0339021 48.276 58 30 0 2 59 912 969 3.01e-12 60.8
Msa0634020 MtrunA17_Chr7g0223531 51.724 58 28 0 2 59 938 995 4.63e-12 60.1
Msa0634020 MtrunA17_Chr8g0352221 50.000 54 27 0 1 54 838 891 6.08e-12 59.7
Msa0634020 MtrunA17_Chr8g0339031 51.852 54 26 0 6 59 933 986 7.61e-12 59.7
Msa0634020 MtrunA17_Chr8g0343781 42.373 59 34 0 1 59 5 63 1.04e-11 55.5
Msa0634020 MtrunA17_Chr8g0338841 45.763 59 32 0 1 59 365 423 2.49e-11 58.2
Msa0634020 MtrunA17_Chr5g0420331 44.828 58 32 0 2 59 923 980 4.14e-11 57.4
Msa0634020 MtrunA17_Chr3g0114381 48.148 54 28 0 1 54 156 209 4.24e-11 57.0
Msa0634020 MtrunA17_Chr5g0420341 46.552 58 31 0 2 59 923 980 5.50e-11 57.0
Msa0634020 MtrunA17_Chr2g0296451 57.778 45 19 0 15 59 454 498 7.56e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATACACTAACCACTTT+TGG 0.342795 4_3:-20659669 Msa0634020:CDS
CCAGCGGAGAATAATGTTAC+TGG 0.394181 4_3:-20658840 Msa0634020:intron
ATAACATCAAATCTAGAATC+TGG 0.419478 4_3:-20659627 Msa0634020:intron
CCTTCTGATATTATGTTGTC+CGG 0.472049 4_3:+20659747 None:intergenic
GAGACATGACAGAACTGTAA+TGG 0.485935 4_3:-20658746 None:intergenic
CCAATCCTCATGGCCAAAAG+TGG 0.541687 4_3:+20659656 None:intergenic
GTTGTCCGGTGAAAAGTGAT+AGG 0.552940 4_3:+20659761 None:intergenic
TTATGCTCTGCCAATCCTCA+TGG 0.558391 4_3:+20659646 None:intergenic
CCGGACAACATAATATCAGA+AGG 0.632034 4_3:-20659747 Msa0634020:CDS
GTCCGGTGAAAAGTGATAGG+CGG 0.732482 4_3:+20659764 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGACATAGAATTATTATA+AGG - chr4_3:20659071-20659090 Msa0634020:intron 15.0%
!!! TTCAATTTACCATTTTAAAT+AGG + chr4_3:20659459-20659478 None:intergenic 15.0%
!!! CTCTATTATTTCAATATTTT+CGG + chr4_3:20659483-20659502 None:intergenic 15.0%
!! TATGACATAGAATTATTATA+AGG - chr4_3:20659071-20659090 Msa0634020:intron 15.0%
!!! TTCAATTTACCATTTTAAAT+AGG + chr4_3:20659459-20659478 None:intergenic 15.0%
!!! CTCTATTATTTCAATATTTT+CGG + chr4_3:20659483-20659502 None:intergenic 15.0%
!! ATAACATCAAATCTAGAATC+TGG - chr4_3:20658896-20658915 Msa0634020:intron 25.0%
! AAGGGAACATAAGTTATATT+AGG - chr4_3:20659343-20659362 Msa0634020:intron 25.0%
! AAGAGAGAATATATACATAG+TGG + chr4_3:20659381-20659400 None:intergenic 25.0%
! TATAAAAAGGAGATATACGT+GGG + chr4_3:20659420-20659439 None:intergenic 25.0%
! TAATACGTGCATGTATAAAA+AGG + chr4_3:20659433-20659452 None:intergenic 25.0%
! GTATTAGCTCCTATTTAAAA+TGG - chr4_3:20659447-20659466 Msa0634020:intron 25.0%
! ATGTATAGAAAAAGAAAGAG+AGG - chr4_3:20659755-20659774 Msa0634020:CDS 25.0%
!! ATAACATCAAATCTAGAATC+TGG - chr4_3:20658896-20658915 Msa0634020:intron 25.0%
! AAGGGAACATAAGTTATATT+AGG - chr4_3:20659343-20659362 Msa0634020:intron 25.0%
! AAGAGAGAATATATACATAG+TGG + chr4_3:20659381-20659400 None:intergenic 25.0%
! TATAAAAAGGAGATATACGT+GGG + chr4_3:20659420-20659439 None:intergenic 25.0%
! TAATACGTGCATGTATAAAA+AGG + chr4_3:20659433-20659452 None:intergenic 25.0%
! GTATTAGCTCCTATTTAAAA+TGG - chr4_3:20659447-20659466 Msa0634020:intron 25.0%
! ATGTATAGAAAAAGAAAGAG+AGG - chr4_3:20659755-20659774 Msa0634020:CDS 25.0%
!!! TGGAAGTTATGGTTGTTTTT+GGG - chr4_3:20658927-20658946 Msa0634020:intron 30.0%
! AACTTATCGATTATTGACTG+AGG - chr4_3:20659163-20659182 Msa0634020:intron 30.0%
! AACAACTTTTAGATGCTCAA+GGG - chr4_3:20659325-20659344 Msa0634020:intron 30.0%
GAATATATACATAGTGGCTA+TGG + chr4_3:20659375-20659394 None:intergenic 30.0%
GTATAAAAAGGAGATATACG+TGG + chr4_3:20659421-20659440 None:intergenic 30.0%
TGGTCATGTAACTGAAAAAT+AGG - chr4_3:20659531-20659550 Msa0634020:intron 30.0%
TCAAACAAAATTCCAACATC+TGG - chr4_3:20659641-20659660 Msa0634020:CDS 30.0%
!!! TGGAAGTTATGGTTGTTTTT+GGG - chr4_3:20658927-20658946 Msa0634020:intron 30.0%
! AACTTATCGATTATTGACTG+AGG - chr4_3:20659163-20659182 Msa0634020:intron 30.0%
! AACAACTTTTAGATGCTCAA+GGG - chr4_3:20659325-20659344 Msa0634020:intron 30.0%
GAATATATACATAGTGGCTA+TGG + chr4_3:20659375-20659394 None:intergenic 30.0%
GTATAAAAAGGAGATATACG+TGG + chr4_3:20659421-20659440 None:intergenic 30.0%
TGGTCATGTAACTGAAAAAT+AGG - chr4_3:20659531-20659550 Msa0634020:intron 30.0%
TCAAACAAAATTCCAACATC+TGG - chr4_3:20659641-20659660 Msa0634020:CDS 30.0%
CCTTCTGATATTATGTTGTC+CGG + chr4_3:20658779-20658798 None:intergenic 35.0%
! ATCTCTTTTATACGCGTGAA+TGG + chr4_3:20658840-20658859 None:intergenic 35.0%
! AGAGATACACTAACCACTTT+TGG - chr4_3:20658854-20658873 Msa0634020:intron 35.0%
! TCTAGAATCTGGTAACAAAG+TGG - chr4_3:20658907-20658926 Msa0634020:intron 35.0%
TGGTAACAAAGTGGAAGTTA+TGG - chr4_3:20658916-20658935 Msa0634020:intron 35.0%
!! GTGGAAGTTATGGTTGTTTT+TGG - chr4_3:20658926-20658945 Msa0634020:intron 35.0%
!!! GGAAGTTATGGTTGTTTTTG+GGG - chr4_3:20658928-20658947 Msa0634020:intron 35.0%
TCAACCAACCAACAAAGAAA+TGG - chr4_3:20658994-20659013 Msa0634020:intron 35.0%
TCTTCCATTTCTTTGTTGGT+TGG + chr4_3:20659001-20659020 None:intergenic 35.0%
AAGGAAGTTGTTACTGTTTC+TGG - chr4_3:20659037-20659056 Msa0634020:intron 35.0%
! GAACAACTTTTAGATGCTCA+AGG - chr4_3:20659324-20659343 Msa0634020:intron 35.0%
! AAAGCTCGTTTTGTCAAACT+TGG - chr4_3:20659505-20659524 Msa0634020:intron 35.0%
! CGTTTTGTCAAACTTGGAAT+TGG - chr4_3:20659511-20659530 Msa0634020:intron 35.0%
AACAAAATTCCAACATCTGG+TGG - chr4_3:20659644-20659663 Msa0634020:CDS 35.0%
ACAAAATTCCAACATCTGGT+GGG - chr4_3:20659645-20659664 Msa0634020:CDS 35.0%
GGATAATATTGATATGCCAG+CGG - chr4_3:20659667-20659686 Msa0634020:CDS 35.0%
CCTTCTGATATTATGTTGTC+CGG + chr4_3:20658779-20658798 None:intergenic 35.0%
! ATCTCTTTTATACGCGTGAA+TGG + chr4_3:20658840-20658859 None:intergenic 35.0%
! AGAGATACACTAACCACTTT+TGG - chr4_3:20658854-20658873 Msa0634020:intron 35.0%
! TCTAGAATCTGGTAACAAAG+TGG - chr4_3:20658907-20658926 Msa0634020:intron 35.0%
TGGTAACAAAGTGGAAGTTA+TGG - chr4_3:20658916-20658935 Msa0634020:intron 35.0%
!! GTGGAAGTTATGGTTGTTTT+TGG - chr4_3:20658926-20658945 Msa0634020:intron 35.0%
!!! GGAAGTTATGGTTGTTTTTG+GGG - chr4_3:20658928-20658947 Msa0634020:intron 35.0%
TCAACCAACCAACAAAGAAA+TGG - chr4_3:20658994-20659013 Msa0634020:intron 35.0%
TCTTCCATTTCTTTGTTGGT+TGG + chr4_3:20659001-20659020 None:intergenic 35.0%
AAGGAAGTTGTTACTGTTTC+TGG - chr4_3:20659037-20659056 Msa0634020:intron 35.0%
! GAACAACTTTTAGATGCTCA+AGG - chr4_3:20659324-20659343 Msa0634020:intron 35.0%
! AAAGCTCGTTTTGTCAAACT+TGG - chr4_3:20659505-20659524 Msa0634020:intron 35.0%
! CGTTTTGTCAAACTTGGAAT+TGG - chr4_3:20659511-20659530 Msa0634020:intron 35.0%
AACAAAATTCCAACATCTGG+TGG - chr4_3:20659644-20659663 Msa0634020:CDS 35.0%
ACAAAATTCCAACATCTGGT+GGG - chr4_3:20659645-20659664 Msa0634020:CDS 35.0%
GGATAATATTGATATGCCAG+CGG - chr4_3:20659667-20659686 Msa0634020:CDS 35.0%
CCGGACAACATAATATCAGA+AGG - chr4_3:20658776-20658795 Msa0634020:CDS 40.0%
!!! AGTTATGGTTGTTTTTGGGG+AGG - chr4_3:20658931-20658950 Msa0634020:intron 40.0%
!!! GTTATGGTTGTTTTTGGGGA+GGG - chr4_3:20658932-20658951 Msa0634020:intron 40.0%
GCAGTCTTCCATTTCTTTGT+TGG + chr4_3:20659005-20659024 None:intergenic 40.0%
!! GTTGTTACTGTTTCTGGTGT+AGG - chr4_3:20659043-20659062 Msa0634020:intron 40.0%
AGATGTGACTGTTGTATCCT+GGG + chr4_3:20659270-20659289 None:intergenic 40.0%
AAGATGTGACTGTTGTATCC+TGG + chr4_3:20659271-20659290 None:intergenic 40.0%
AAACCTTGCTAATTCACGTG+CGG + chr4_3:20659301-20659320 None:intergenic 40.0%
ACGTGAATTAGCAAGGTTTC+AGG - chr4_3:20659302-20659321 Msa0634020:intron 40.0%
AAAAGGAGATATACGTGGGT+TGG + chr4_3:20659416-20659435 None:intergenic 40.0%
CAAAATTCCAACATCTGGTG+GGG - chr4_3:20659646-20659665 Msa0634020:CDS 40.0%
ATATTATCCCCACCAGATGT+TGG + chr4_3:20659656-20659675 None:intergenic 40.0%
CCGGACAACATAATATCAGA+AGG - chr4_3:20658776-20658795 Msa0634020:CDS 40.0%
!!! AGTTATGGTTGTTTTTGGGG+AGG - chr4_3:20658931-20658950 Msa0634020:intron 40.0%
!!! GTTATGGTTGTTTTTGGGGA+GGG - chr4_3:20658932-20658951 Msa0634020:intron 40.0%
GCAGTCTTCCATTTCTTTGT+TGG + chr4_3:20659005-20659024 None:intergenic 40.0%
!! GTTGTTACTGTTTCTGGTGT+AGG - chr4_3:20659043-20659062 Msa0634020:intron 40.0%
AGATGTGACTGTTGTATCCT+GGG + chr4_3:20659270-20659289 None:intergenic 40.0%
AAGATGTGACTGTTGTATCC+TGG + chr4_3:20659271-20659290 None:intergenic 40.0%
AAACCTTGCTAATTCACGTG+CGG + chr4_3:20659301-20659320 None:intergenic 40.0%
ACGTGAATTAGCAAGGTTTC+AGG - chr4_3:20659302-20659321 Msa0634020:intron 40.0%
AAAAGGAGATATACGTGGGT+TGG + chr4_3:20659416-20659435 None:intergenic 40.0%
CAAAATTCCAACATCTGGTG+GGG - chr4_3:20659646-20659665 Msa0634020:CDS 40.0%
ATATTATCCCCACCAGATGT+TGG + chr4_3:20659656-20659675 None:intergenic 40.0%
GTTGTCCGGTGAAAAGTGAT+AGG + chr4_3:20658765-20658784 None:intergenic 45.0%
!! ACTAACCACTTTTGGCCATG+AGG - chr4_3:20658862-20658881 Msa0634020:intron 45.0%
TTATGCTCTGCCAATCCTCA+TGG + chr4_3:20658880-20658899 None:intergenic 45.0%
AGACTGCAATGCAGTAGACA+AGG - chr4_3:20659018-20659037 Msa0634020:intron 45.0%
!! TTAAGAGTATTTCGCCACCC+CGG - chr4_3:20659205-20659224 Msa0634020:intron 45.0%
AGCTATGAGACCTATCGCAT+CGG + chr4_3:20659240-20659259 None:intergenic 45.0%
TCTCATAGCTAATACTCCCC+AGG - chr4_3:20659250-20659269 Msa0634020:intron 45.0%
GATGTGACTGTTGTATCCTG+GGG + chr4_3:20659269-20659288 None:intergenic 45.0%
CCAGTAACATTATTCTCCGC+TGG + chr4_3:20659686-20659705 None:intergenic 45.0%
CCAGCGGAGAATAATGTTAC+TGG - chr4_3:20659683-20659702 Msa0634020:CDS 45.0%
GTTGTCCGGTGAAAAGTGAT+AGG + chr4_3:20658765-20658784 None:intergenic 45.0%
!! ACTAACCACTTTTGGCCATG+AGG - chr4_3:20658862-20658881 Msa0634020:intron 45.0%
TTATGCTCTGCCAATCCTCA+TGG + chr4_3:20658880-20658899 None:intergenic 45.0%
AGACTGCAATGCAGTAGACA+AGG - chr4_3:20659018-20659037 Msa0634020:intron 45.0%
!! TTAAGAGTATTTCGCCACCC+CGG - chr4_3:20659205-20659224 Msa0634020:intron 45.0%
AGCTATGAGACCTATCGCAT+CGG + chr4_3:20659240-20659259 None:intergenic 45.0%
TCTCATAGCTAATACTCCCC+AGG - chr4_3:20659250-20659269 Msa0634020:intron 45.0%
GATGTGACTGTTGTATCCTG+GGG + chr4_3:20659269-20659288 None:intergenic 45.0%
CCAGTAACATTATTCTCCGC+TGG + chr4_3:20659686-20659705 None:intergenic 45.0%
CCAGCGGAGAATAATGTTAC+TGG - chr4_3:20659683-20659702 Msa0634020:CDS 45.0%
!! CACCGCCTATCACTTTTCAC+CGG - chr4_3:20658757-20658776 Msa0634020:CDS 50.0%
GTCCGGTGAAAAGTGATAGG+CGG + chr4_3:20658762-20658781 None:intergenic 50.0%
CCAATCCTCATGGCCAAAAG+TGG + chr4_3:20658870-20658889 None:intergenic 50.0%
!! CCACTTTTGGCCATGAGGAT+TGG - chr4_3:20658867-20658886 Msa0634020:intron 50.0%
ATCGCATCGGGTAACCTATC+CGG + chr4_3:20659227-20659246 None:intergenic 50.0%
GATAGGTTACCCGATGCGAT+AGG - chr4_3:20659227-20659246 Msa0634020:intron 50.0%
GCTATGAGACCTATCGCATC+GGG + chr4_3:20659239-20659258 None:intergenic 50.0%
!! CACCGCCTATCACTTTTCAC+CGG - chr4_3:20658757-20658776 Msa0634020:CDS 50.0%
GTCCGGTGAAAAGTGATAGG+CGG + chr4_3:20658762-20658781 None:intergenic 50.0%
CCAATCCTCATGGCCAAAAG+TGG + chr4_3:20658870-20658889 None:intergenic 50.0%
!! CCACTTTTGGCCATGAGGAT+TGG - chr4_3:20658867-20658886 Msa0634020:intron 50.0%
ATCGCATCGGGTAACCTATC+CGG + chr4_3:20659227-20659246 None:intergenic 50.0%
GATAGGTTACCCGATGCGAT+AGG - chr4_3:20659227-20659246 Msa0634020:intron 50.0%
GCTATGAGACCTATCGCATC+GGG + chr4_3:20659239-20659258 None:intergenic 50.0%
! TGAGGTGTGCACGAAGCACT+GGG - chr4_3:20659181-20659200 Msa0634020:intron 55.0%
AGTATTTCGCCACCCCGGAT+AGG - chr4_3:20659210-20659229 Msa0634020:intron 55.0%
TCGCATCGGGTAACCTATCC+GGG + chr4_3:20659226-20659245 None:intergenic 55.0%
GCACCGCACGTGAATTAGCA+AGG - chr4_3:20659295-20659314 Msa0634020:intron 55.0%
! TGAGGTGTGCACGAAGCACT+GGG - chr4_3:20659181-20659200 Msa0634020:intron 55.0%
AGTATTTCGCCACCCCGGAT+AGG - chr4_3:20659210-20659229 Msa0634020:intron 55.0%
TCGCATCGGGTAACCTATCC+GGG + chr4_3:20659226-20659245 None:intergenic 55.0%
GCACCGCACGTGAATTAGCA+AGG - chr4_3:20659295-20659314 Msa0634020:intron 55.0%
CTGAGGTGTGCACGAAGCAC+TGG - chr4_3:20659180-20659199 Msa0634020:intron 60.0%
ATCGGGTAACCTATCCGGGG+TGG + chr4_3:20659222-20659241 None:intergenic 60.0%
CGCATCGGGTAACCTATCCG+GGG + chr4_3:20659225-20659244 None:intergenic 60.0%
CTGAGGTGTGCACGAAGCAC+TGG - chr4_3:20659180-20659199 Msa0634020:intron 60.0%
ATCGGGTAACCTATCCGGGG+TGG + chr4_3:20659222-20659241 None:intergenic 60.0%
CGCATCGGGTAACCTATCCG+GGG + chr4_3:20659225-20659244 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 20658749 20659796 20658749 ID=Msa0634020;Name=Msa0634020
chr4_3 mRNA 20658749 20659796 20658749 ID=Msa0634020-mRNA-1;Parent=Msa0634020;Name=Msa0634020-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|0|0|2|0|92
chr4_3 exon 20659628 20659796 20659628 ID=Msa0634020-mRNA-1:exon:5464;Parent=Msa0634020-mRNA-1
chr4_3 exon 20658749 20658858 20658749 ID=Msa0634020-mRNA-1:exon:5463;Parent=Msa0634020-mRNA-1
chr4_3 CDS 20659628 20659796 20659628 ID=Msa0634020-mRNA-1:cds;Parent=Msa0634020-mRNA-1
chr4_3 CDS 20658749 20658858 20658749 ID=Msa0634020-mRNA-1:cds;Parent=Msa0634020-mRNA-1
Gene Sequence

>Msa0634020

ATGTTGTTCACCGCCTATCACTTTTCACCGGACAACATAATATCAGAAGGTTGTCAAAGTGTGTTGATCATAAATCACACAAAGACAACCATTCACGCGTATAAAAGAGATACACTAACCACTTTTGGCCATGAGGATTGGCAGAGCATAACATCAAATCTAGAATCTGCGGAGAATAATGTTACTGGTCGAGAGCAAGATGAAAATATAAGTGAAGATATGCACGAACATGATGCAATGTATAGAAAAAGAAAGAGAGGAGACATGACAGAACTGTAA

Protein sequence

>Msa0634020

MLFTAYHFSPDNIISEGCQSVLIINHTKTTIHAYKRDTLTTFGHEDWQSITSNLESAENNVTGREQDENISEDMHEHDAMYRKRKRGDMTEL*