AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0537500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537500 MtrunA17_Chr4g0008981 46.939 147 23 4 1 92 10 156 6.02e-32 108
Msa0537500 MtrunA17_Chr4g0009241 69.492 59 18 0 1 59 1446 1504 6.88e-24 94.0
Msa0537500 MtrunA17_Chr4g0009141 71.930 57 16 0 1 57 1442 1498 8.97e-23 90.9
Msa0537500 MtrunA17_Chr4g0009171 69.091 55 17 0 1 55 1647 1701 1.11e-20 84.7
Msa0537500 MtrunA17_Chr4g0009401 67.273 55 18 0 1 55 1424 1478 2.87e-20 83.6
Msa0537500 MtrunA17_Chr4g0009011 63.158 57 21 0 1 57 210 266 2.79e-19 80.1
Msa0537500 MtrunA17_Chr4g0009211 64.912 57 20 0 1 57 10 66 3.84e-19 76.3
Msa0537500 MtrunA17_Chr4g0009041 67.273 55 18 0 1 55 1430 1484 6.46e-19 79.7
Msa0537500 MtrunA17_Chr4g0009361 70.370 54 16 0 2 55 439 492 1.43e-18 78.6
Msa0537500 MtrunA17_Chr4g0009101 63.158 57 21 0 1 57 1458 1514 2.63e-18 78.2
Msa0537500 MtrunA17_Chr4g0009301 63.636 55 20 0 1 55 1462 1516 1.37e-17 75.9
Msa0537500 MtrunA17_Chr4g0005871 48.276 58 30 0 1 58 1055 1112 1.13e-14 67.8
Msa0537500 MtrunA17_Chr5g0414761 55.556 54 24 0 6 59 712 765 4.67e-14 65.9
Msa0537500 MtrunA17_Chr3g0085531 48.276 58 30 0 2 59 120 177 4.97e-14 65.5
Msa0537500 MtrunA17_Chr4g0005591 46.552 58 31 0 1 58 1007 1064 1.62e-13 64.3
Msa0537500 MtrunA17_Chr4g0005891 46.552 58 31 0 1 58 979 1036 1.91e-13 64.3
Msa0537500 MtrunA17_Chr3g0085731 52.830 53 25 0 2 54 930 982 2.54e-13 63.9
Msa0537500 MtrunA17_Chr8g0352211 52.830 53 25 0 2 54 852 904 2.82e-13 63.5
Msa0537500 MtrunA17_Chr4g0005851 44.828 58 32 0 1 58 965 1022 3.53e-13 63.5
Msa0537500 MtrunA17_Chr8g0370511 50.000 58 29 0 2 59 922 979 3.57e-13 63.5
Msa0537500 MtrunA17_Chr8g0339011 50.000 54 27 0 6 59 921 974 4.21e-13 63.2
Msa0537500 MtrunA17_Chr8g0338981 51.852 54 26 0 6 59 925 978 5.82e-13 62.8
Msa0537500 MtrunA17_Chr8g0338881 55.102 49 22 0 11 59 962 1010 7.16e-13 62.4
Msa0537500 MtrunA17_Chr8g0339021 48.276 58 30 0 2 59 912 969 3.01e-12 60.8
Msa0537500 MtrunA17_Chr7g0223531 51.724 58 28 0 2 59 938 995 4.63e-12 60.1
Msa0537500 MtrunA17_Chr8g0352221 50.000 54 27 0 1 54 838 891 6.08e-12 59.7
Msa0537500 MtrunA17_Chr8g0339031 51.852 54 26 0 6 59 933 986 7.61e-12 59.7
Msa0537500 MtrunA17_Chr8g0343781 42.373 59 34 0 1 59 5 63 1.04e-11 55.5
Msa0537500 MtrunA17_Chr8g0338841 45.763 59 32 0 1 59 365 423 2.49e-11 58.2
Msa0537500 MtrunA17_Chr5g0420331 44.828 58 32 0 2 59 923 980 4.14e-11 57.4
Msa0537500 MtrunA17_Chr3g0114381 48.148 54 28 0 1 54 156 209 4.24e-11 57.0
Msa0537500 MtrunA17_Chr5g0420341 46.552 58 31 0 2 59 923 980 5.50e-11 57.0
Msa0537500 MtrunA17_Chr2g0296451 57.778 45 19 0 15 59 454 498 7.56e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGATACACTAACCACTTT+TGG 0.342795 4_1:-16203399 Msa0537500:CDS
CCAGCGGAGAATAATGTTAC+TGG 0.394181 4_1:-16202570 Msa0537500:intron
ATAACATCAAATCTAGAATC+TGG 0.419478 4_1:-16203357 Msa0537500:intron
CCTTCTGATATTATGTTGTC+CGG 0.472049 4_1:+16203477 None:intergenic
GAGACATGACAGAACTGTAA+TGG 0.485935 4_1:-16202476 None:intergenic
CCAATCCTCATGGCCAAAAG+TGG 0.541687 4_1:+16203386 None:intergenic
GTTGTCCGGTGAAAAGTGAT+AGG 0.552940 4_1:+16203491 None:intergenic
TTATGCTCTGCCAATCCTCA+TGG 0.558391 4_1:+16203376 None:intergenic
CCGGACAACATAATATCAGA+AGG 0.632034 4_1:-16203477 Msa0537500:CDS
GTCCGGTGAAAAGTGATAGG+CGG 0.732482 4_1:+16203494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGACATAGAATTATTATA+AGG - chr4_1:16202801-16202820 Msa0537500:intron 15.0%
!!! TTCAATTTACCATTTTAAAT+AGG + chr4_1:16203189-16203208 None:intergenic 15.0%
!!! CTCTATTATTTCAATATTTT+CGG + chr4_1:16203213-16203232 None:intergenic 15.0%
!! TATGACATAGAATTATTATA+AGG - chr4_1:16202801-16202820 Msa0537500:intron 15.0%
!!! TTCAATTTACCATTTTAAAT+AGG + chr4_1:16203189-16203208 None:intergenic 15.0%
!!! CTCTATTATTTCAATATTTT+CGG + chr4_1:16203213-16203232 None:intergenic 15.0%
!! ATAACATCAAATCTAGAATC+TGG - chr4_1:16202626-16202645 Msa0537500:intron 25.0%
! AAGGGAACATAAGTTATATT+AGG - chr4_1:16203073-16203092 Msa0537500:intron 25.0%
! AAGAGAGAATATATACATAG+TGG + chr4_1:16203111-16203130 None:intergenic 25.0%
! TATAAAAAGGAGATATACGT+GGG + chr4_1:16203150-16203169 None:intergenic 25.0%
! TAATACGTGCATGTATAAAA+AGG + chr4_1:16203163-16203182 None:intergenic 25.0%
! GTATTAGCTCCTATTTAAAA+TGG - chr4_1:16203177-16203196 Msa0537500:intron 25.0%
! ATGTATAGAAAAAGAAAGAG+AGG - chr4_1:16203485-16203504 Msa0537500:CDS 25.0%
!! ATAACATCAAATCTAGAATC+TGG - chr4_1:16202626-16202645 Msa0537500:intron 25.0%
! AAGGGAACATAAGTTATATT+AGG - chr4_1:16203073-16203092 Msa0537500:intron 25.0%
! AAGAGAGAATATATACATAG+TGG + chr4_1:16203111-16203130 None:intergenic 25.0%
! TATAAAAAGGAGATATACGT+GGG + chr4_1:16203150-16203169 None:intergenic 25.0%
! TAATACGTGCATGTATAAAA+AGG + chr4_1:16203163-16203182 None:intergenic 25.0%
! GTATTAGCTCCTATTTAAAA+TGG - chr4_1:16203177-16203196 Msa0537500:intron 25.0%
! ATGTATAGAAAAAGAAAGAG+AGG - chr4_1:16203485-16203504 Msa0537500:CDS 25.0%
!!! TGGAAGTTATGGTTGTTTTT+GGG - chr4_1:16202657-16202676 Msa0537500:intron 30.0%
! AACTTATCGATTATTGACTG+AGG - chr4_1:16202893-16202912 Msa0537500:intron 30.0%
! AACAACTTTTAGATGCTCAA+GGG - chr4_1:16203055-16203074 Msa0537500:intron 30.0%
GAATATATACATAGTGGCTA+TGG + chr4_1:16203105-16203124 None:intergenic 30.0%
GTATAAAAAGGAGATATACG+TGG + chr4_1:16203151-16203170 None:intergenic 30.0%
TGGTCATGTAACTGAAAAAT+AGG - chr4_1:16203261-16203280 Msa0537500:intron 30.0%
TCAAACAAAATTCCAACATC+TGG - chr4_1:16203371-16203390 Msa0537500:CDS 30.0%
!!! TGGAAGTTATGGTTGTTTTT+GGG - chr4_1:16202657-16202676 Msa0537500:intron 30.0%
! AACTTATCGATTATTGACTG+AGG - chr4_1:16202893-16202912 Msa0537500:intron 30.0%
! AACAACTTTTAGATGCTCAA+GGG - chr4_1:16203055-16203074 Msa0537500:intron 30.0%
GAATATATACATAGTGGCTA+TGG + chr4_1:16203105-16203124 None:intergenic 30.0%
GTATAAAAAGGAGATATACG+TGG + chr4_1:16203151-16203170 None:intergenic 30.0%
TGGTCATGTAACTGAAAAAT+AGG - chr4_1:16203261-16203280 Msa0537500:intron 30.0%
TCAAACAAAATTCCAACATC+TGG - chr4_1:16203371-16203390 Msa0537500:CDS 30.0%
CCTTCTGATATTATGTTGTC+CGG + chr4_1:16202509-16202528 None:intergenic 35.0%
! ATCTCTTTTATACGCGTGAA+TGG + chr4_1:16202570-16202589 None:intergenic 35.0%
! AGAGATACACTAACCACTTT+TGG - chr4_1:16202584-16202603 Msa0537500:intron 35.0%
! TCTAGAATCTGGTAACAAAG+TGG - chr4_1:16202637-16202656 Msa0537500:intron 35.0%
TGGTAACAAAGTGGAAGTTA+TGG - chr4_1:16202646-16202665 Msa0537500:intron 35.0%
!! GTGGAAGTTATGGTTGTTTT+TGG - chr4_1:16202656-16202675 Msa0537500:intron 35.0%
!!! GGAAGTTATGGTTGTTTTTG+GGG - chr4_1:16202658-16202677 Msa0537500:intron 35.0%
TCAACCAACCAACAAAGAAA+TGG - chr4_1:16202724-16202743 Msa0537500:intron 35.0%
TCTTCCATTTCTTTGTTGGT+TGG + chr4_1:16202731-16202750 None:intergenic 35.0%
AAGGAAGTTGTTACTGTTTC+TGG - chr4_1:16202767-16202786 Msa0537500:intron 35.0%
! GAACAACTTTTAGATGCTCA+AGG - chr4_1:16203054-16203073 Msa0537500:intron 35.0%
! AAAGCTCGTTTTGTCAAACT+TGG - chr4_1:16203235-16203254 Msa0537500:intron 35.0%
! CGTTTTGTCAAACTTGGAAT+TGG - chr4_1:16203241-16203260 Msa0537500:intron 35.0%
AACAAAATTCCAACATCTGG+TGG - chr4_1:16203374-16203393 Msa0537500:CDS 35.0%
ACAAAATTCCAACATCTGGT+GGG - chr4_1:16203375-16203394 Msa0537500:CDS 35.0%
GGATAATATTGATATGCCAG+CGG - chr4_1:16203397-16203416 Msa0537500:CDS 35.0%
CCTTCTGATATTATGTTGTC+CGG + chr4_1:16202509-16202528 None:intergenic 35.0%
! ATCTCTTTTATACGCGTGAA+TGG + chr4_1:16202570-16202589 None:intergenic 35.0%
! AGAGATACACTAACCACTTT+TGG - chr4_1:16202584-16202603 Msa0537500:intron 35.0%
! TCTAGAATCTGGTAACAAAG+TGG - chr4_1:16202637-16202656 Msa0537500:intron 35.0%
TGGTAACAAAGTGGAAGTTA+TGG - chr4_1:16202646-16202665 Msa0537500:intron 35.0%
!! GTGGAAGTTATGGTTGTTTT+TGG - chr4_1:16202656-16202675 Msa0537500:intron 35.0%
!!! GGAAGTTATGGTTGTTTTTG+GGG - chr4_1:16202658-16202677 Msa0537500:intron 35.0%
TCAACCAACCAACAAAGAAA+TGG - chr4_1:16202724-16202743 Msa0537500:intron 35.0%
TCTTCCATTTCTTTGTTGGT+TGG + chr4_1:16202731-16202750 None:intergenic 35.0%
AAGGAAGTTGTTACTGTTTC+TGG - chr4_1:16202767-16202786 Msa0537500:intron 35.0%
! GAACAACTTTTAGATGCTCA+AGG - chr4_1:16203054-16203073 Msa0537500:intron 35.0%
! AAAGCTCGTTTTGTCAAACT+TGG - chr4_1:16203235-16203254 Msa0537500:intron 35.0%
! CGTTTTGTCAAACTTGGAAT+TGG - chr4_1:16203241-16203260 Msa0537500:intron 35.0%
AACAAAATTCCAACATCTGG+TGG - chr4_1:16203374-16203393 Msa0537500:CDS 35.0%
ACAAAATTCCAACATCTGGT+GGG - chr4_1:16203375-16203394 Msa0537500:CDS 35.0%
GGATAATATTGATATGCCAG+CGG - chr4_1:16203397-16203416 Msa0537500:CDS 35.0%
CCGGACAACATAATATCAGA+AGG - chr4_1:16202506-16202525 Msa0537500:CDS 40.0%
!!! AGTTATGGTTGTTTTTGGGG+AGG - chr4_1:16202661-16202680 Msa0537500:intron 40.0%
!!! GTTATGGTTGTTTTTGGGGA+GGG - chr4_1:16202662-16202681 Msa0537500:intron 40.0%
GCAGTCTTCCATTTCTTTGT+TGG + chr4_1:16202735-16202754 None:intergenic 40.0%
!! GTTGTTACTGTTTCTGGTGT+AGG - chr4_1:16202773-16202792 Msa0537500:intron 40.0%
AGATGTGACTGTTGTATCCT+GGG + chr4_1:16203000-16203019 None:intergenic 40.0%
AAGATGTGACTGTTGTATCC+TGG + chr4_1:16203001-16203020 None:intergenic 40.0%
AAACCTTGCTAATTCACGTG+CGG + chr4_1:16203031-16203050 None:intergenic 40.0%
ACGTGAATTAGCAAGGTTTC+AGG - chr4_1:16203032-16203051 Msa0537500:intron 40.0%
AAAAGGAGATATACGTGGGT+TGG + chr4_1:16203146-16203165 None:intergenic 40.0%
CAAAATTCCAACATCTGGTG+GGG - chr4_1:16203376-16203395 Msa0537500:CDS 40.0%
ATATTATCCCCACCAGATGT+TGG + chr4_1:16203386-16203405 None:intergenic 40.0%
CCGGACAACATAATATCAGA+AGG - chr4_1:16202506-16202525 Msa0537500:CDS 40.0%
!!! AGTTATGGTTGTTTTTGGGG+AGG - chr4_1:16202661-16202680 Msa0537500:intron 40.0%
!!! GTTATGGTTGTTTTTGGGGA+GGG - chr4_1:16202662-16202681 Msa0537500:intron 40.0%
GCAGTCTTCCATTTCTTTGT+TGG + chr4_1:16202735-16202754 None:intergenic 40.0%
!! GTTGTTACTGTTTCTGGTGT+AGG - chr4_1:16202773-16202792 Msa0537500:intron 40.0%
AGATGTGACTGTTGTATCCT+GGG + chr4_1:16203000-16203019 None:intergenic 40.0%
AAGATGTGACTGTTGTATCC+TGG + chr4_1:16203001-16203020 None:intergenic 40.0%
AAACCTTGCTAATTCACGTG+CGG + chr4_1:16203031-16203050 None:intergenic 40.0%
ACGTGAATTAGCAAGGTTTC+AGG - chr4_1:16203032-16203051 Msa0537500:intron 40.0%
AAAAGGAGATATACGTGGGT+TGG + chr4_1:16203146-16203165 None:intergenic 40.0%
CAAAATTCCAACATCTGGTG+GGG - chr4_1:16203376-16203395 Msa0537500:CDS 40.0%
ATATTATCCCCACCAGATGT+TGG + chr4_1:16203386-16203405 None:intergenic 40.0%
GTTGTCCGGTGAAAAGTGAT+AGG + chr4_1:16202495-16202514 None:intergenic 45.0%
!! ACTAACCACTTTTGGCCATG+AGG - chr4_1:16202592-16202611 Msa0537500:intron 45.0%
TTATGCTCTGCCAATCCTCA+TGG + chr4_1:16202610-16202629 None:intergenic 45.0%
AGACTGCAATGCAGTAGACA+AGG - chr4_1:16202748-16202767 Msa0537500:intron 45.0%
!! TTAAGAGTATTTCGCCACCC+CGG - chr4_1:16202935-16202954 Msa0537500:intron 45.0%
AGCTATGAGACCTATCGCAT+CGG + chr4_1:16202970-16202989 None:intergenic 45.0%
TCTCATAGCTAATACTCCCC+AGG - chr4_1:16202980-16202999 Msa0537500:intron 45.0%
GATGTGACTGTTGTATCCTG+GGG + chr4_1:16202999-16203018 None:intergenic 45.0%
CCAGTAACATTATTCTCCGC+TGG + chr4_1:16203416-16203435 None:intergenic 45.0%
CCAGCGGAGAATAATGTTAC+TGG - chr4_1:16203413-16203432 Msa0537500:CDS 45.0%
GTTGTCCGGTGAAAAGTGAT+AGG + chr4_1:16202495-16202514 None:intergenic 45.0%
!! ACTAACCACTTTTGGCCATG+AGG - chr4_1:16202592-16202611 Msa0537500:intron 45.0%
TTATGCTCTGCCAATCCTCA+TGG + chr4_1:16202610-16202629 None:intergenic 45.0%
AGACTGCAATGCAGTAGACA+AGG - chr4_1:16202748-16202767 Msa0537500:intron 45.0%
!! TTAAGAGTATTTCGCCACCC+CGG - chr4_1:16202935-16202954 Msa0537500:intron 45.0%
AGCTATGAGACCTATCGCAT+CGG + chr4_1:16202970-16202989 None:intergenic 45.0%
TCTCATAGCTAATACTCCCC+AGG - chr4_1:16202980-16202999 Msa0537500:intron 45.0%
GATGTGACTGTTGTATCCTG+GGG + chr4_1:16202999-16203018 None:intergenic 45.0%
CCAGTAACATTATTCTCCGC+TGG + chr4_1:16203416-16203435 None:intergenic 45.0%
CCAGCGGAGAATAATGTTAC+TGG - chr4_1:16203413-16203432 Msa0537500:CDS 45.0%
!! CACCGCCTATCACTTTTCAC+CGG - chr4_1:16202487-16202506 Msa0537500:CDS 50.0%
GTCCGGTGAAAAGTGATAGG+CGG + chr4_1:16202492-16202511 None:intergenic 50.0%
CCAATCCTCATGGCCAAAAG+TGG + chr4_1:16202600-16202619 None:intergenic 50.0%
!! CCACTTTTGGCCATGAGGAT+TGG - chr4_1:16202597-16202616 Msa0537500:intron 50.0%
ATCGCATCGGGTAACCTATC+CGG + chr4_1:16202957-16202976 None:intergenic 50.0%
GATAGGTTACCCGATGCGAT+AGG - chr4_1:16202957-16202976 Msa0537500:intron 50.0%
GCTATGAGACCTATCGCATC+GGG + chr4_1:16202969-16202988 None:intergenic 50.0%
!! CACCGCCTATCACTTTTCAC+CGG - chr4_1:16202487-16202506 Msa0537500:CDS 50.0%
GTCCGGTGAAAAGTGATAGG+CGG + chr4_1:16202492-16202511 None:intergenic 50.0%
CCAATCCTCATGGCCAAAAG+TGG + chr4_1:16202600-16202619 None:intergenic 50.0%
!! CCACTTTTGGCCATGAGGAT+TGG - chr4_1:16202597-16202616 Msa0537500:intron 50.0%
ATCGCATCGGGTAACCTATC+CGG + chr4_1:16202957-16202976 None:intergenic 50.0%
GATAGGTTACCCGATGCGAT+AGG - chr4_1:16202957-16202976 Msa0537500:intron 50.0%
GCTATGAGACCTATCGCATC+GGG + chr4_1:16202969-16202988 None:intergenic 50.0%
! TGAGGTGTGCACGAAGCACT+GGG - chr4_1:16202911-16202930 Msa0537500:intron 55.0%
AGTATTTCGCCACCCCGGAT+AGG - chr4_1:16202940-16202959 Msa0537500:intron 55.0%
TCGCATCGGGTAACCTATCC+GGG + chr4_1:16202956-16202975 None:intergenic 55.0%
GCACCGCACGTGAATTAGCA+AGG - chr4_1:16203025-16203044 Msa0537500:intron 55.0%
! TGAGGTGTGCACGAAGCACT+GGG - chr4_1:16202911-16202930 Msa0537500:intron 55.0%
AGTATTTCGCCACCCCGGAT+AGG - chr4_1:16202940-16202959 Msa0537500:intron 55.0%
TCGCATCGGGTAACCTATCC+GGG + chr4_1:16202956-16202975 None:intergenic 55.0%
GCACCGCACGTGAATTAGCA+AGG - chr4_1:16203025-16203044 Msa0537500:intron 55.0%
CTGAGGTGTGCACGAAGCAC+TGG - chr4_1:16202910-16202929 Msa0537500:intron 60.0%
ATCGGGTAACCTATCCGGGG+TGG + chr4_1:16202952-16202971 None:intergenic 60.0%
CGCATCGGGTAACCTATCCG+GGG + chr4_1:16202955-16202974 None:intergenic 60.0%
CTGAGGTGTGCACGAAGCAC+TGG - chr4_1:16202910-16202929 Msa0537500:intron 60.0%
ATCGGGTAACCTATCCGGGG+TGG + chr4_1:16202952-16202971 None:intergenic 60.0%
CGCATCGGGTAACCTATCCG+GGG + chr4_1:16202955-16202974 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 16202479 16203526 16202479 ID=Msa0537500;Name=Msa0537500
chr4_1 mRNA 16202479 16203526 16202479 ID=Msa0537500-mRNA-1;Parent=Msa0537500;Name=Msa0537500-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|0|0|2|0|92
chr4_1 exon 16203358 16203526 16203358 ID=Msa0537500-mRNA-1:exon:4869;Parent=Msa0537500-mRNA-1
chr4_1 exon 16202479 16202588 16202479 ID=Msa0537500-mRNA-1:exon:4868;Parent=Msa0537500-mRNA-1
chr4_1 CDS 16203358 16203526 16203358 ID=Msa0537500-mRNA-1:cds;Parent=Msa0537500-mRNA-1
chr4_1 CDS 16202479 16202588 16202479 ID=Msa0537500-mRNA-1:cds;Parent=Msa0537500-mRNA-1
Gene Sequence

>Msa0537500

ATGTTGTTCACCGCCTATCACTTTTCACCGGACAACATAATATCAGAAGGTTGTCAAAGTGTGTTGATCATAAATCACACAAAGACAACCATTCACGCGTATAAAAGAGATACACTAACCACTTTTGGCCATGAGGATTGGCAGAGCATAACATCAAATCTAGAATCTGCGGAGAATAATGTTACTGGTCGAGAGCAAGATGAAAATATAAGTGAAGATATGCACGAACATGATGCAATGTATAGAAAAAGAAAGAGAGGAGACATGACAGAACTGTAA

Protein sequence

>Msa0537500

MLFTAYHFSPDNIISEGCQSVLIINHTKTTIHAYKRDTLTTFGHEDWQSITSNLESAENNVTGREQDENISEDMHEHDAMYRKRKRGDMTEL*