AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0635260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0635260 MtrunA17_Chr4g0014781 88.730 559 59 1 1 555 1 559 0.0 1006
Msa0635260 MtrunA17_Chr4g0014801 88.909 559 58 1 1 555 1 559 0.0 994
Msa0635260 MtrunA17_Chr4g0014791 88.036 560 59 3 1 555 1 557 0.0 989
Msa0635260 MtrunA17_Chr4g0014871 85.027 561 78 2 1 555 1 561 0.0 986
Msa0635260 MtrunA17_Chr4g0014841 87.478 559 64 2 1 555 1 557 0.0 979
Msa0635260 MtrunA17_Chr4g0014861 85.484 434 62 1 121 553 1 434 0.0 776
Msa0635260 MtrunA17_Chr3g0077151 66.971 548 170 4 5 550 8 546 0.0 726
Msa0635260 MtrunA17_Chr8g0353031 48.066 543 250 7 26 547 19 550 3.27e-165 481
Msa0635260 MtrunA17_Chr7g0241741 42.315 553 294 7 16 547 13 561 2.09e-157 461
Msa0635260 MtrunA17_Chr8g0375311 41.533 561 296 8 16 550 31 585 2.01e-155 457
Msa0635260 MtrunA17_Chr7g0261811 41.416 565 307 6 11 554 13 574 2.64e-154 454
Msa0635260 MtrunA17_Chr4g0008651 41.410 553 295 8 17 548 26 570 6.10e-153 451
Msa0635260 MtrunA17_Chr8g0375291 42.962 547 284 8 16 538 31 573 2.38e-152 449
Msa0635260 MtrunA17_Chr4g0005901 39.929 566 315 6 4 548 11 572 3.69e-152 448
Msa0635260 MtrunA17_Chr3g0104011 41.885 573 297 9 1 547 1 563 2.53e-151 446
Msa0635260 MtrunA17_Chr7g0240381 40.901 555 291 9 16 548 20 559 3.27e-149 441
Msa0635260 MtrunA17_Chr3g0112671 40.325 553 293 9 16 547 24 560 1.48e-148 439
Msa0635260 MtrunA17_Chr7g0261791 41.336 554 300 6 16 547 20 570 1.87e-147 437
Msa0635260 MtrunA17_Chr7g0261881 38.761 565 322 6 11 554 13 574 6.27e-147 436
Msa0635260 MtrunA17_Chr3g0114501 40.975 554 306 7 16 550 13 564 3.14e-146 433
Msa0635260 MtrunA17_Chr3g0112611 39.929 566 314 8 2 547 10 569 2.07e-145 431
Msa0635260 MtrunA17_Chr8g0375351 40.998 561 299 8 16 550 31 585 2.28e-145 431
Msa0635260 MtrunA17_Chr1g0166791 40.949 569 307 9 1 547 3 564 1.06e-144 429
Msa0635260 MtrunA17_Chr1g0166711 42.077 568 304 8 1 547 1 564 1.10e-144 429
Msa0635260 MtrunA17_Chr8g0375341 40.925 562 298 10 16 551 31 584 1.29e-143 427
Msa0635260 MtrunA17_Chr1g0187241 40.000 570 315 8 1 547 1 566 4.33e-142 423
Msa0635260 MtrunA17_Chr7g0261771 39.964 553 308 7 16 547 22 571 1.62e-140 419
Msa0635260 MtrunA17_Chr8g0375361 39.286 588 318 8 1 553 4 587 2.28e-139 416
Msa0635260 MtrunA17_Chr8g0375331 38.947 570 315 9 1 544 1 563 3.25e-138 412
Msa0635260 MtrunA17_Chr8g0375321 38.664 569 317 8 2 544 14 576 5.12e-138 412
Msa0635260 MtrunA17_Chr1g0166741 38.434 549 305 8 1 527 3 540 9.43e-133 399
Msa0635260 MtrunA17_Chr7g0261851 38.489 556 312 6 16 547 19 568 1.43e-131 396
Msa0635260 MtrunA17_Chr1g0166771 40.079 509 272 8 44 530 10 507 4.44e-127 383
Msa0635260 MtrunA17_Chr3g0082631 43.656 465 228 8 107 549 9 461 9.99e-126 377
Msa0635260 MtrunA17_Chr5g0399071 39.789 568 307 10 17 555 17 578 3.66e-125 379
Msa0635260 MtrunA17_Chr5g0399061 38.339 566 318 8 17 555 19 580 1.45e-124 377
Msa0635260 MtrunA17_Chr4g0056431 38.462 572 312 8 17 555 17 581 9.09e-123 373
Msa0635260 MtrunA17_Chr5g0422881 37.030 559 320 8 24 555 19 572 1.69e-122 372
Msa0635260 MtrunA17_Chr7g0255621 36.890 553 314 11 25 549 20 565 3.53e-118 362
Msa0635260 MtrunA17_Chr6g0451841 38.710 527 287 9 44 548 24 536 8.29e-118 359
Msa0635260 MtrunA17_Chr3g0142101 37.294 547 310 9 16 541 26 560 9.11e-118 360
Msa0635260 MtrunA17_Chr7g0255611 35.816 564 329 10 1 537 7 564 1.30e-117 360
Msa0635260 MtrunA17_Chr7g0217461 37.587 572 320 12 16 555 15 581 3.76e-115 353
Msa0635260 MtrunA17_Chr7g0214461 34.062 549 330 9 19 544 72 611 1.72e-110 343
Msa0635260 MtrunA17_Chr5g0415791 35.548 557 317 9 25 547 29 577 2.49e-110 342
Msa0635260 MtrunA17_Chr7g0255601 34.615 572 340 10 3 547 8 572 1.20e-109 340
Msa0635260 MtrunA17_Chr1g0166731 41.447 456 242 8 112 546 9 460 1.33e-109 335
Msa0635260 MtrunA17_Chr4g0063591 39.964 548 300 8 16 541 13 553 1.33e-108 336
Msa0635260 MtrunA17_Chr5g0400371 35.140 572 332 11 3 544 93 655 3.75e-108 338
Msa0635260 MtrunA17_Chr4g0054891 35.727 571 323 13 2 542 90 646 1.53e-107 336
Msa0635260 MtrunA17_Chr7g0261661 39.474 456 252 6 113 547 1 453 1.96e-107 331
Msa0635260 MtrunA17_Chr1g0213711 34.347 559 325 11 23 555 19 561 7.69e-106 329
Msa0635260 MtrunA17_Chr3g0142091 35.765 562 324 11 16 554 17 564 9.42e-106 329
Msa0635260 MtrunA17_Chr4g0059971 35.949 548 319 9 31 548 40 585 6.98e-105 327
Msa0635260 MtrunA17_Chr8g0362711 35.638 564 322 9 16 546 15 570 9.91e-105 326
Msa0635260 MtrunA17_Chr5g0415961 34.266 572 336 10 16 554 15 579 2.69e-103 322
Msa0635260 MtrunA17_Chr7g0261751 37.821 468 232 8 16 426 22 487 6.06e-102 317
Msa0635260 MtrunA17_Chr7g0218851 33.274 559 323 12 19 544 40 581 1.07e-101 319
Msa0635260 MtrunA17_Chr2g0276751 36.445 557 322 11 16 554 29 571 1.10e-101 319
Msa0635260 MtrunA17_Chr4g0059991 33.390 584 332 12 1 546 8 572 3.08e-100 315
Msa0635260 MtrunA17_Chr2g0284701 33.220 590 349 12 1 547 1 588 8.42e-100 314
Msa0635260 MtrunA17_Chr1g0157611 34.643 560 327 10 15 544 4 554 3.64e-99 311
Msa0635260 MtrunA17_Chr8g0390331 34.046 561 333 12 12 544 2 553 4.79e-99 311
Msa0635260 MtrunA17_Chr3g0133341 33.818 550 332 9 21 547 10 550 1.51e-98 310
Msa0635260 MtrunA17_Chr5g0439381 36.445 557 314 10 14 547 17 556 1.20e-97 308
Msa0635260 MtrunA17_Chr8g0362781 33.218 578 346 10 8 551 14 585 2.09e-97 307
Msa0635260 MtrunA17_Chr1g0157601 34.559 544 319 11 29 544 19 553 1.01e-96 305
Msa0635260 MtrunA17_Chr4g0059981 34.134 583 337 11 1 548 12 582 1.01e-94 301
Msa0635260 MtrunA17_Chr1g0147631 33.099 568 344 11 2 544 8 564 3.86e-94 299
Msa0635260 MtrunA17_Chr4g0011041 31.429 560 345 9 16 544 27 578 4.16e-93 296
Msa0635260 MtrunA17_Chr7g0214731 31.843 559 344 10 26 551 62 616 2.42e-92 296
Msa0635260 MtrunA17_Chr8g0379971 32.973 555 317 11 16 518 11 562 2.87e-92 295
Msa0635260 MtrunA17_Chr1g0157621 34.100 522 310 8 49 544 4 517 3.58e-91 290
Msa0635260 MtrunA17_Chr4g0060001 32.316 557 342 8 23 551 27 576 1.19e-90 290
Msa0635260 MtrunA17_Chr4g0003761 33.333 552 333 12 21 546 27 569 1.85e-89 287
Msa0635260 MtrunA17_Chr7g0240271 30.503 577 360 9 1 545 1 568 2.01e-89 286
Msa0635260 MtrunA17_Chr2g0331521 31.657 537 333 10 36 544 33 563 4.26e-89 286
Msa0635260 MtrunA17_Chr4g0011471 32.197 528 321 9 46 544 37 556 2.56e-88 283
Msa0635260 MtrunA17_Chr2g0331471 33.566 572 335 15 10 546 68 629 1.09e-87 285
Msa0635260 MtrunA17_Chr2g0331481 31.882 574 343 13 10 546 4 566 1.17e-86 281
Msa0635260 MtrunA17_Chr4g0069581 34.086 487 288 10 83 548 44 518 6.82e-86 276
Msa0635260 MtrunA17_Chr1g0193391 31.550 542 344 10 25 549 17 548 9.73e-85 275
Msa0635260 MtrunA17_Chr2g0331511 30.726 537 338 8 36 544 32 562 1.18e-84 275
Msa0635260 MtrunA17_Chr8g0379991 34.336 565 307 11 54 555 9 572 1.87e-84 273
Msa0635260 MtrunA17_Chr1g0177591 32.482 548 333 15 17 546 36 564 2.00e-84 273
Msa0635260 MtrunA17_Chr7g0271221 31.919 542 342 10 25 549 17 548 1.58e-83 271
Msa0635260 MtrunA17_Chr2g0331461 31.524 571 349 13 10 546 4 566 2.34e-81 267
Msa0635260 MtrunA17_Chr8g0362801 34.483 464 268 8 115 547 3 461 2.50e-81 262
Msa0635260 MtrunA17_Chr7g0240281 30.743 579 357 9 1 545 2 570 1.34e-80 264
Msa0635260 MtrunA17_Chr7g0261731 40.122 329 174 6 16 323 22 348 3.94e-77 248
Msa0635260 MtrunA17_Chr2g0326211 32.911 553 317 13 26 548 20 548 4.83e-75 248
Msa0635260 MtrunA17_Chr3g0112631 42.593 270 149 3 283 547 4 272 3.34e-74 238
Msa0635260 MtrunA17_Chr5g0415841 33.487 433 262 7 133 547 35 459 2.70e-73 241
Msa0635260 MtrunA17_Chr2g0325951 31.171 555 330 12 23 548 17 548 2.61e-72 241
Msa0635260 MtrunA17_Chr2g0325961 31.598 557 326 11 23 548 17 549 6.43e-71 237
Msa0635260 MtrunA17_Chr1g0177611 32.080 452 272 14 113 546 1 435 1.13e-67 225
Msa0635260 MtrunA17_Chr7g0235521 31.626 449 271 8 123 545 19 457 2.02e-67 226
Msa0635260 MtrunA17_Chr7g0240131 31.403 449 272 8 123 545 19 457 2.42e-66 223
Msa0635260 MtrunA17_Chr7g0240211 31.403 449 272 8 123 545 19 457 3.07e-66 223
Msa0635260 MtrunA17_Chr7g0240181 31.403 449 272 8 123 545 19 457 3.11e-66 223
Msa0635260 MtrunA17_Chr1g0177631 31.416 452 275 13 113 546 1 435 3.94e-62 211
Msa0635260 MtrunA17_Chr4g0060071 38.745 271 161 4 278 547 32 298 9.35e-61 203
Msa0635260 MtrunA17_Chr7g0240151 30.067 449 260 9 123 545 19 439 1.04e-59 205
Msa0635260 MtrunA17_Chr4g0014851 79.310 116 19 1 1 111 1 116 2.87e-58 191
Msa0635260 MtrunA17_Chr3g0142111 41.473 258 140 3 16 264 37 292 5.56e-58 197
Msa0635260 MtrunA17_Chr7g0234181 30.993 413 262 7 133 544 23 413 1.15e-54 190
Msa0635260 MtrunA17_Chr7g0234151 30.524 439 276 8 112 544 3 418 2.81e-54 189
Msa0635260 MtrunA17_Chr7g0234381 30.137 438 279 8 112 544 3 418 7.26e-53 185
Msa0635260 MtrunA17_Chr7g0261721 42.500 200 114 1 348 547 3 201 1.30e-50 174
Msa0635260 MtrunA17_Chr3g0142121 37.603 242 141 4 300 541 31 262 5.91e-50 174
Msa0635260 MtrunA17_Chr3g0112651 40.260 154 81 3 112 257 21 171 9.76e-32 122
Msa0635260 MtrunA17_Chr1g0166721 48.649 111 57 0 1 111 1 111 2.33e-28 110
Msa0635260 MtrunA17_Chr3g0112661 44.248 113 62 1 16 128 22 133 5.25e-28 108
Msa0635260 MtrunA17_Chr1g0147591 30.303 198 122 5 137 320 3 198 1.84e-25 103
Msa0635260 MtrunA17_Chr7g0261671 43.750 96 54 0 16 111 23 118 1.81e-23 95.9
Msa0635260 MtrunA17_Chr2g0326221 28.261 230 135 3 318 547 24 223 3.50e-21 92.4
Msa0635260 MtrunA17_Chr3g0082621 51.389 72 35 0 40 111 1 72 6.70e-20 84.7
Msa0635260 MtrunA17_Chr4g0060061 34.783 115 74 1 132 245 14 128 1.84e-19 84.7
Msa0635260 MtrunA17_Chr7g0261831 34.014 147 88 2 247 384 3 149 1.18e-16 79.0
Msa0635260 MtrunA17_Chr1g0147581 36.842 114 64 2 409 521 10 116 3.39e-16 75.1
Msa0635260 MtrunA17_Chr7g0240221 27.523 109 75 1 3 111 9 113 1.07e-12 65.1
Msa0635260 MtrunA17_Chr7g0240191 27.523 109 75 1 3 111 9 113 1.07e-12 65.1
Msa0635260 MtrunA17_Chr7g0240161 27.523 109 75 1 3 111 9 113 1.07e-12 65.1
Msa0635260 MtrunA17_Chr7g0240141 26.606 109 76 1 3 111 9 113 4.49e-12 63.2
Msa0635260 MtrunA17_Chr7g0261821 42.857 63 36 0 485 547 1 63 2.66e-11 59.3
Msa0635260 MtrunA17_Chr4g0060051 34.409 93 61 0 16 108 13 105 2.66e-11 61.2
Msa0635260 MtrunA17_Chr8g0362791 36.364 77 49 0 31 107 42 118 8.71e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0635260 AT1G22540.1 66.294 537 169 2 16 545 19 550 0.0 694
Msa0635260 AT1G72125.1 59.180 561 220 6 1 552 1 561 0.0 639
Msa0635260 AT1G72120.1 59.498 558 211 8 1 548 1 553 0.0 619
Msa0635260 AT1G72140.1 57.509 546 202 5 16 547 23 552 0.0 613
Msa0635260 AT1G22550.1 56.584 562 223 5 2 548 5 560 0.0 601
Msa0635260 AT1G22570.1 56.383 564 214 7 10 548 5 561 0.0 586
Msa0635260 AT1G22570.2 56.629 528 205 6 38 548 4 524 0.0 553
Msa0635260 AT1G72130.1 53.947 532 223 6 25 551 23 537 0.0 544
Msa0635260 AT3G54450.1 45.304 543 267 8 25 547 10 542 7.66e-168 488
Msa0635260 AT1G72130.2 52.693 427 181 5 129 551 10 419 3.10e-150 438
Msa0635260 AT1G72130.3 52.693 427 181 5 129 551 10 419 3.10e-150 438
Msa0635260 AT5G01180.2 39.336 572 319 7 1 550 1 566 1.61e-149 441
Msa0635260 AT5G01180.3 39.336 572 319 7 1 550 1 566 1.61e-149 441
Msa0635260 AT5G01180.1 39.336 572 319 7 1 550 1 566 1.61e-149 441
Msa0635260 AT3G54140.1 40.422 569 309 8 1 546 1 562 2.22e-148 439
Msa0635260 AT3G54140.2 40.422 569 309 8 1 546 1 562 2.22e-148 439
Msa0635260 AT2G37900.1 41.607 560 279 11 16 547 27 566 4.26e-146 433
Msa0635260 AT3G53960.1 41.622 555 292 9 17 547 27 573 6.08e-146 433
Msa0635260 AT2G02040.1 40.351 570 312 8 5 550 19 584 6.22e-146 433
Msa0635260 AT1G62200.1 41.516 554 285 8 11 537 36 577 1.12e-142 425
Msa0635260 AT1G62200.2 39.794 583 283 10 11 537 36 606 1.62e-136 410
Msa0635260 AT2G02020.1 40.893 560 287 11 1 546 13 542 2.24e-136 407
Msa0635260 AT1G62200.3 39.794 583 283 10 11 537 50 620 3.28e-136 409
Msa0635260 AT2G40460.1 40.328 548 306 7 16 547 13 555 7.91e-136 407
Msa0635260 AT5G46040.1 39.474 532 299 5 16 527 16 544 7.38e-133 399
Msa0635260 AT5G46050.1 38.879 553 313 6 16 547 16 564 1.38e-131 396
Msa0635260 AT5G14940.1 39.779 543 295 9 25 544 5 538 8.19e-129 388
Msa0635260 AT2G37900.2 41.502 506 248 11 70 547 2 487 1.10e-127 383
Msa0635260 AT2G02020.2 39.893 559 273 13 1 546 13 521 9.83e-123 371
Msa0635260 AT3G01350.1 38.669 556 303 11 29 555 9 555 3.27e-119 363
Msa0635260 AT1G68570.1 36.979 576 323 8 2 547 3 568 7.41e-116 356
Msa0635260 AT1G68570.2 37.989 537 298 7 41 547 7 538 4.73e-115 353
Msa0635260 AT1G69870.1 37.276 558 305 10 24 547 48 594 7.85e-115 354
Msa0635260 AT1G12110.1 35.506 583 334 10 3 555 7 577 9.82e-113 348
Msa0635260 AT2G26690.1 38.727 550 303 9 16 541 13 552 1.97e-111 344
Msa0635260 AT1G32450.1 34.392 567 335 9 10 544 20 581 7.95e-109 338
Msa0635260 AT4G21680.1 36.898 561 319 13 16 546 17 572 6.95e-108 335
Msa0635260 AT1G18880.1 33.844 588 336 12 1 554 3 571 2.69e-103 323
Msa0635260 AT1G52190.1 34.871 542 315 9 36 544 30 566 1.33e-102 322
Msa0635260 AT2G26690.2 38.140 527 292 9 39 541 3 519 8.39e-102 318
Msa0635260 AT3G21670.1 36.871 556 310 11 17 547 19 558 3.10e-101 318
Msa0635260 AT3G16180.1 35.009 557 322 12 21 544 16 565 7.08e-101 317
Msa0635260 AT1G59740.1 35.677 569 327 12 2 542 15 572 4.53e-100 315
Msa0635260 AT5G46050.2 37.665 454 258 6 115 547 3 452 8.67e-99 308
Msa0635260 AT1G69850.1 34.095 569 333 10 17 547 15 579 2.74e-98 310
Msa0635260 AT1G27040.2 33.880 549 339 7 17 546 13 556 3.53e-98 309
Msa0635260 AT1G27040.1 33.880 549 339 7 17 546 17 560 3.73e-98 309
Msa0635260 AT2G26690.3 37.791 516 287 9 50 541 2 507 3.87e-97 305
Msa0635260 AT5G62680.1 33.757 551 326 10 31 547 47 592 6.51e-96 305
Msa0635260 AT5G62730.1 32.504 563 355 9 1 547 32 585 1.01e-95 303
Msa0635260 AT1G33440.1 35.921 554 317 10 16 544 27 567 1.31e-94 301
Msa0635260 AT3G47960.1 34.173 556 315 12 31 547 65 608 6.32e-94 300
Msa0635260 AT1G27080.1 33.877 552 323 10 29 552 16 553 1.26e-93 298
Msa0635260 AT1G27040.3 34.156 527 323 7 39 546 7 528 2.62e-93 295
Msa0635260 AT5G19640.1 33.088 544 315 14 28 544 60 581 3.06e-90 290
Msa0635260 AT5G62730.2 32.203 531 337 8 33 547 3 526 4.25e-90 287
Msa0635260 AT5G13400.1 31.272 566 349 9 16 547 52 611 1.00e-87 283
Msa0635260 AT5G28470.2 32.014 556 339 13 24 550 14 559 1.27e-86 279
Msa0635260 AT1G69860.1 34.062 549 315 11 31 550 22 552 1.68e-86 278
Msa0635260 AT1G27080.2 33.843 523 304 10 58 552 3 511 2.32e-86 277
Msa0635260 AT5G28470.1 31.835 556 338 14 24 550 14 557 6.28e-84 272
Msa0635260 AT3G25280.1 32.364 550 325 12 10 544 4 521 1.49e-79 259
Msa0635260 AT3G25260.1 33.151 549 318 13 9 544 3 515 2.39e-78 256
Msa0635260 AT3G45650.1 30.123 571 349 15 4 551 2 545 2.65e-73 244
Msa0635260 AT3G45680.1 31.373 561 325 15 20 551 15 544 5.93e-71 238
Msa0635260 AT3G45710.1 30.521 557 320 12 27 548 21 545 2.76e-70 236
Msa0635260 AT3G45700.1 30.106 568 334 15 13 551 1 534 1.63e-69 233
Msa0635260 AT3G45660.1 31.599 519 308 13 54 548 48 543 4.15e-69 233
Msa0635260 AT3G45660.3 31.599 519 308 13 54 548 48 543 4.15e-69 233
Msa0635260 AT3G45660.2 31.407 519 309 13 54 548 69 564 6.49e-69 233
Msa0635260 AT3G45650.2 28.811 597 349 16 4 551 2 571 7.97e-68 230
Msa0635260 AT3G45720.1 30.713 547 316 15 36 551 28 542 1.42e-64 221
Msa0635260 AT5G11570.1 28.077 520 298 11 36 544 22 476 4.76e-64 217
Msa0635260 AT3G45690.1 29.291 536 292 15 26 536 17 490 1.81e-55 195
Msa0635260 AT2G38100.2 29.245 530 309 17 55 554 2 495 9.38e-50 179
Msa0635260 AT2G38100.1 29.245 530 309 17 55 554 18 511 1.69e-49 179
Msa0635260 AT2G38100.3 29.245 530 309 17 55 554 24 517 2.18e-49 179

Find 124 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTGTGTTCAAGCTTTC+GGG 0.149446 4_3:+23589531 Msa0635260:CDS
CTTCTAGCTTTGTGCTCTTT+AGG 0.162338 4_3:-23589578 None:intergenic
TGGGCTACAACTTTAGTTTA+TGG 0.166899 4_3:+23590860 Msa0635260:CDS
ATTTGAAATGATTAATATTT+AGG 0.169062 4_3:-23587983 None:intergenic
TGTTAATATCAAATAATATA+TGG 0.172615 4_3:+23591636 Msa0635260:intron
CTAGAAGCTCATTCTTTAAT+TGG 0.175630 4_3:+23589594 Msa0635260:CDS
GGACATTTATTGACGGATTA+TGG 0.187042 4_3:+23591752 Msa0635260:three_prime_UTR
TACTAAGATTGATTCCAATT+TGG 0.197203 4_3:+23590840 Msa0635260:CDS
GCTTCCCTCCTTCCTCTCTT+AGG 0.216834 4_3:+23588518 Msa0635260:CDS
GATAACATTAGTTGGGTTCT+TGG 0.227701 4_3:+23589677 Msa0635260:CDS
GCTACACGTTTCATCTTCTT+TGG 0.238477 4_3:-23590789 None:intergenic
GACATATAGGTTTAGCATTA+AGG 0.243452 4_3:+23589760 Msa0635260:CDS
TGCTTCTCTTATCTATATAT+TGG 0.248624 4_3:+23588589 Msa0635260:CDS
ATTAGTTGGGTTCTTGGATT+TGG 0.259493 4_3:+23589683 Msa0635260:CDS
GAAAGACAACAATTTCAAAA+TGG 0.266196 4_3:-23591708 None:intergenic
CAACACAGGTTTCTTTATTA+AGG 0.267002 4_3:-23587958 None:intergenic
TTAGATCCGAATCTGGTTAT+TGG 0.269969 4_3:+23588126 Msa0635260:CDS
CCCATGCTTCTTAGTTCATA+TGG 0.276199 4_3:-23591310 None:intergenic
TAACTTATCAACAACGGATT+TGG 0.276561 4_3:+23589850 Msa0635260:CDS
AAACCTTGTGTTCAAGCTTT+CGG 0.280299 4_3:+23589530 Msa0635260:CDS
ACTAAGATTGATTCCAATTT+GGG 0.287288 4_3:+23590841 Msa0635260:CDS
CTATACAATTTAACCTTAAT+CGG 0.298042 4_3:-23591580 None:intergenic
ACTAAAGTTGTAGCCCAAAT+TGG 0.304028 4_3:-23590854 None:intergenic
GCATGCAAATGCTCTATATT+TGG 0.310305 4_3:+23591668 Msa0635260:three_prime_UTR
AGGAAGGAGGGAAGCAGTTC+CGG 0.311487 4_3:-23588510 None:intergenic
AAGAGTCCCTTTCTTAGAAT+TGG 0.315382 4_3:+23589791 Msa0635260:CDS
CTTGCTGGATTAAGTGTGTT+GGG 0.320199 4_3:+23591484 Msa0635260:CDS
CTGCAAAGAATGTCAATATT+TGG 0.326443 4_3:+23588486 Msa0635260:CDS
ACAAGGATAGTCACCGATTA+AGG 0.328728 4_3:+23591567 Msa0635260:exon
GCACTCTATTTGAGTATTGT+TGG 0.352342 4_3:+23591337 Msa0635260:CDS
GTTAATATCAAATAATATAT+GGG 0.355240 4_3:+23591637 Msa0635260:intron
ATCACAATGCTTCAAAGAAT+TGG 0.359553 4_3:+23591076 Msa0635260:CDS
AAAGAAGTGAATTATTCAAT+AGG 0.359764 4_3:+23588026 Msa0635260:five_prime_UTR
ATGAAGAGAACAATATTTCC+TGG 0.360937 4_3:+23590932 Msa0635260:CDS
AAAGACAACAATTTCAAAAT+GGG 0.361065 4_3:-23591707 None:intergenic
TTGTTGCAGCTATAAGAAAT+TGG 0.372408 4_3:+23589822 Msa0635260:CDS
GCGGAGAGGGTATCATATTA+TGG 0.374011 4_3:+23588404 Msa0635260:CDS
TCATCTTCTTTGGATCCATT+TGG 0.384209 4_3:-23590779 None:intergenic
CTCATTGGAACAGTTGCAAT+TGG 0.397891 4_3:-23591196 None:intergenic
CAACTGTTCCAATGAGTATA+TGG 0.398189 4_3:+23591203 Msa0635260:CDS
CGTTATCTACGATCGCATAT+TGG 0.401583 4_3:+23591015 Msa0635260:CDS
CTTCATAGAACTCCCTTGCT+TGG 0.417446 4_3:-23590915 None:intergenic
GCATCTCTTCAAACTATAAA+CGG 0.426930 4_3:+23590971 Msa0635260:CDS
CCACTAGCAAGATCTATAAC+TGG 0.427746 4_3:+23591040 Msa0635260:CDS
AGATCCGCTTGGTTTATCAT+AGG 0.431139 4_3:+23588149 Msa0635260:CDS
GAAAATATTAGTGGCAAAGA+TGG 0.431214 4_3:+23591400 Msa0635260:CDS
AACTTGATATCGTATCTAAC+AGG 0.435560 4_3:+23588437 Msa0635260:CDS
TGCTTGGAACAAGGACATAT+AGG 0.439390 4_3:+23589747 Msa0635260:CDS
ACTTGCTGGATTAAGTGTGT+TGG 0.452550 4_3:+23591483 Msa0635260:CDS
TTTATAGTTTGAAGAGATGC+AGG 0.456396 4_3:-23590968 None:intergenic
AAATAATATATGGGATTGAA+TGG 0.460451 4_3:+23591646 Msa0635260:three_prime_UTR
TGTCGTAATGATCATTGCTT+TGG 0.461713 4_3:+23589715 Msa0635260:CDS
CCATGCTTCTTAGTTCATAT+GGG 0.470030 4_3:-23591309 None:intergenic
ATATACAAGATAACATTAGT+TGG 0.471533 4_3:+23589669 Msa0635260:CDS
ACTGAGGAGAACAATATGTA+AGG 0.472580 4_3:-23589448 None:intergenic
GAAGCTCATTCTTTAATTGG+TGG 0.474451 4_3:+23589597 Msa0635260:CDS
AAGGCATTGCTAACACCAAA+TGG 0.474748 4_3:+23590764 Msa0635260:CDS
CCCGCAGTTAGATCCGAATC+TGG 0.475981 4_3:+23588119 Msa0635260:CDS
TACACGACCAATTCTAAGAA+AGG 0.479274 4_3:-23589798 None:intergenic
ACCAACCACCATATACTCAT+TGG 0.479829 4_3:-23591211 None:intergenic
AAGAATGTCAATATTTGGTC+CGG 0.480278 4_3:+23588491 Msa0635260:CDS
TTTACATTCTTCACCAAGCA+AGG 0.480785 4_3:+23590902 Msa0635260:CDS
GGATCTCCAATAACCAGATT+CGG 0.483507 4_3:-23588132 None:intergenic
GTATCATATTATGGAATTCA+AGG 0.491184 4_3:+23588413 Msa0635260:CDS
TATACAAGATAACATTAGTT+GGG 0.492477 4_3:+23589670 Msa0635260:CDS
GCAAAGATGGACATGATAGT+TGG 0.492895 4_3:+23591413 Msa0635260:CDS
TGTTGAGATTAAAAGACTAA+AGG 0.497601 4_3:+23591144 Msa0635260:CDS
TCTTTGATAGAAAATATTAG+TGG 0.498259 4_3:+23591391 Msa0635260:CDS
AGGTGTGGAAGTGGCGGAGA+GGG 0.511145 4_3:+23588391 Msa0635260:intron
GTTGCAGTGCTTTGTTTGAG+TGG 0.521993 4_3:-23588461 None:intergenic
ACACGACCAATTCTAAGAAA+GGG 0.526584 4_3:-23589797 None:intergenic
CACAGGTTTCTTTATTAAGG+TGG 0.528545 4_3:-23587955 None:intergenic
TCACTCTATCTAGTTGCCAT+TGG 0.531734 4_3:+23589494 Msa0635260:CDS
CTGGTTATTGGAGATCCGCT+TGG 0.544926 4_3:+23588138 Msa0635260:CDS
TTGCTGGATTAAGTGTGTTG+GGG 0.546286 4_3:+23591485 Msa0635260:CDS
TAGTGAGGTGGAAGAAGCAA+AGG 0.547050 4_3:+23590814 Msa0635260:CDS
CCCATATGAACTAAGAAGCA+TGG 0.549394 4_3:+23591309 Msa0635260:CDS
TGAAGCATTGTGATACCTGA+GGG 0.553587 4_3:-23591067 None:intergenic
TCCAATGAGTATATGGTGGT+TGG 0.556157 4_3:+23591210 Msa0635260:CDS
TCTATAACTGGAAAACCCTC+AGG 0.558530 4_3:+23591052 Msa0635260:CDS
CGAAAGCACCTAAGAGAGGA+AGG 0.559132 4_3:-23588526 None:intergenic
ATGCTTCAAAGAATTGGCAC+TGG 0.559271 4_3:+23591082 Msa0635260:CDS
TTAGCTCTTGTTGATTACAA+AGG 0.561962 4_3:+23588092 Msa0635260:CDS
GCCCCGAAAGCTTGAACACA+AGG 0.567096 4_3:-23589533 None:intergenic
CCAGTTATAGATCTTGCTAG+TGG 0.572059 4_3:-23591040 None:intergenic
AGCACCTAAGAGAGGAAGGA+GGG 0.574375 4_3:-23588522 None:intergenic
CCAGATTCGGATCTAACTGC+GGG 0.574874 4_3:-23588119 None:intergenic
GGTGTTGCAAGCTTTCTAAG+TGG 0.575161 4_3:+23591358 Msa0635260:CDS
GCGAAATAACTTATCAACAA+CGG 0.576311 4_3:+23589844 Msa0635260:CDS
GGAGAACAATATGTAAGGGA+AGG 0.577683 4_3:-23589443 None:intergenic
TTTATGCCCACCTTGTCCAA+TGG 0.578469 4_3:-23589510 None:intergenic
CCATATGAACTAAGAAGCAT+GGG 0.586670 4_3:+23591310 Msa0635260:CDS
TATCTAGTTGCCATTGGACA+AGG 0.587068 4_3:+23589500 Msa0635260:CDS
TATCATATTATGGAATTCAA+GGG 0.587902 4_3:+23588414 Msa0635260:CDS
TTGTTTAGGTGTGGAAGTGG+CGG 0.589530 4_3:+23588385 Msa0635260:intron
CATTGGTAGCTTCCTCAACA+AGG 0.590057 4_3:+23590745 Msa0635260:intron
GCAATGATCATTACGACACA+AGG 0.592495 4_3:-23589710 None:intergenic
CTGAGGAGAACAATATGTAA+GGG 0.592564 4_3:-23589447 None:intergenic
TTGAAGCATTGTGATACCTG+AGG 0.594873 4_3:-23591068 None:intergenic
TTACATTCTTCACCAAGCAA+GGG 0.595454 4_3:+23590903 Msa0635260:CDS
AACGTGTAGCTTTAGTGAGG+TGG 0.596536 4_3:+23590802 Msa0635260:CDS
ACCTTGTGTTCAAGCTTTCG+GGG 0.599219 4_3:+23589532 Msa0635260:CDS
AAGCACCTAAGAGAGGAAGG+AGG 0.605702 4_3:-23588523 None:intergenic
AAGACAACAATTTCAAAATG+GGG 0.607260 4_3:-23591706 None:intergenic
GCAACGAAAGCACCTAAGAG+AGG 0.607557 4_3:-23588530 None:intergenic
GAGAACAATATGTAAGGGAA+GGG 0.609361 4_3:-23589442 None:intergenic
CATAAAAGTGTCATCACACA+AGG 0.610250 4_3:+23591550 Msa0635260:CDS
AATAAGATTACTTGTGACTG+AGG 0.611966 4_3:-23589464 None:intergenic
CTAGTTGCCATTGGACAAGG+TGG 0.620501 4_3:+23589503 Msa0635260:CDS
ATAGTTTGAAGAGATGCAGG+AGG 0.623068 4_3:-23590965 None:intergenic
GGATACAAATTACAGGGACT+AGG 0.624524 4_3:+23589395 Msa0635260:intron
GCAGGAGGTATGTCAAAACC+AGG 0.635394 4_3:-23590950 None:intergenic
TAGGTGTGGAAGTGGCGGAG+AGG 0.640474 4_3:+23588390 Msa0635260:intron
CTAAAGGTAGCACAAGAGCA+TGG 0.641511 4_3:+23591160 Msa0635260:CDS
ACCAGATTCGGATCTAACTG+CGG 0.646716 4_3:-23588120 None:intergenic
TTAATATTTAGGCACAACAC+AGG 0.656519 4_3:-23587972 None:intergenic
TAGTTGCCATTGGACAAGGT+GGG 0.664534 4_3:+23589504 Msa0635260:CDS
GTGTTAGCAATGCCTTGTTG+AGG 0.678778 4_3:-23590757 None:intergenic
CAATGATCATTACGACACAA+GGG 0.688557 4_3:-23589709 None:intergenic
GATTTGGAAGAAGAATGTGA+TGG 0.693639 4_3:+23589866 Msa0635260:CDS
TTGTGTTCAAGCTTTCGGGG+CGG 0.695110 4_3:+23589535 Msa0635260:CDS
TGAAACGTGTAGCTTTAGTG+AGG 0.701058 4_3:+23590799 Msa0635260:CDS
TGTACCTATGATAAACCAAG+CGG 0.704715 4_3:-23588153 None:intergenic
CTGTTCCAATGAGTATATGG+TGG 0.715883 4_3:+23591206 Msa0635260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGAAATGATTAATATTT+AGG - chr4_3:23587986-23588005 None:intergenic 10.0%
!!! ATTTGTTTTTATCATAATAA+AGG + chr4_3:23588190-23588209 Msa0635260:intron 10.0%
!!! TTTTTACGTTTTTTTTTAAA+TGG + chr4_3:23588332-23588351 Msa0635260:intron 10.0%
!! TGTATGATATATTAAAAAAA+TGG + chr4_3:23589328-23589347 Msa0635260:intron 10.0%
!!! AATTTTTCTATCTATATTTA+CGG + chr4_3:23591105-23591124 Msa0635260:CDS 10.0%
!! TGTTAATATCAAATAATATA+TGG + chr4_3:23591636-23591655 Msa0635260:intron 10.0%
!! GTTAATATCAAATAATATAT+GGG + chr4_3:23591637-23591656 Msa0635260:intron 10.0%
!! AATGAGATATAAAATTGATT+TGG - chr4_3:23588288-23588307 None:intergenic 15.0%
!!! TTTTTACACTTTAAAATGAA+TGG + chr4_3:23588649-23588668 Msa0635260:intron 15.0%
!!! AAAGAATTAATTTTGACTAA+TGG - chr4_3:23588802-23588821 None:intergenic 15.0%
!!! AAATGATTCTCAAATAATTT+TGG - chr4_3:23588934-23588953 None:intergenic 15.0%
!!! ATGTAGTTATTTCTTTTAAA+TGG + chr4_3:23590341-23590360 Msa0635260:intron 15.0%
!!! CTTTCAATGTTTTAATTTAT+TGG - chr4_3:23590691-23590710 None:intergenic 15.0%
!! ATTAAAACATTGAAAGATTA+TGG + chr4_3:23590695-23590714 Msa0635260:intron 15.0%
!! AAAGAAGTGAATTATTCAAT+AGG + chr4_3:23588026-23588045 Msa0635260:five_prime_UTR 20.0%
!!! TTATTCAATAGGTTTTTTGA+TGG + chr4_3:23588037-23588056 Msa0635260:exon 20.0%
!!! GTTTTTATCATAATAAAGGT+TGG + chr4_3:23588194-23588213 Msa0635260:intron 20.0%
!!! AGATATAAAATTGATTTGGT+AGG - chr4_3:23588284-23588303 None:intergenic 20.0%
!! TATCATATTATGGAATTCAA+GGG + chr4_3:23588414-23588433 Msa0635260:CDS 20.0%
!!! TTCATTTTAGGAGAAATTTT+CGG + chr4_3:23588713-23588732 Msa0635260:intron 20.0%
!! ATATACAAGATAACATTAGT+TGG + chr4_3:23589669-23589688 Msa0635260:CDS 20.0%
!! TATACAAGATAACATTAGTT+GGG + chr4_3:23589670-23589689 Msa0635260:CDS 20.0%
!!! GATCAAATTCATTGATTATA+TGG - chr4_3:23590211-23590230 None:intergenic 20.0%
!! TGTTATGATCACTATATAAA+AGG - chr4_3:23590635-23590654 None:intergenic 20.0%
!! TCTTTGATAGAAAATATTAG+TGG + chr4_3:23591391-23591410 Msa0635260:CDS 20.0%
!!! CACATCTTGATTATTTTTAT+TGG + chr4_3:23591458-23591477 Msa0635260:CDS 20.0%
!! CTATACAATTTAACCTTAAT+CGG - chr4_3:23591583-23591602 None:intergenic 20.0%
!! AAATAATATATGGGATTGAA+TGG + chr4_3:23591646-23591665 Msa0635260:three_prime_UTR 20.0%
!! AAAGACAACAATTTCAAAAT+GGG - chr4_3:23591710-23591729 None:intergenic 20.0%
!!! ATACTTTTACCTATGAAAAA+AGG + chr4_3:23591731-23591750 Msa0635260:three_prime_UTR 20.0%
!!! AATAAATGTCCTTTTTTCAT+AGG - chr4_3:23591743-23591762 None:intergenic 20.0%
!!! GAATTATGCATGTTTTGTTT+AGG + chr4_3:23588371-23588390 Msa0635260:intron 25.0%
! GTATCATATTATGGAATTCA+AGG + chr4_3:23588413-23588432 Msa0635260:CDS 25.0%
! TGCTTCTCTTATCTATATAT+TGG + chr4_3:23588589-23588608 Msa0635260:CDS 25.0%
! CTAAAATGAACTATTAACGA+AGG - chr4_3:23588703-23588722 None:intergenic 25.0%
!! CTTCGTTAATAGTTCATTTT+AGG + chr4_3:23588701-23588720 Msa0635260:intron 25.0%
!!! AAAATCTGAAAGTATCTTGA+AGG + chr4_3:23589054-23589073 Msa0635260:intron 25.0%
!!! AAATCTGAAAGTATCTTGAA+GGG + chr4_3:23589055-23589074 Msa0635260:intron 25.0%
! TTGCACAAATAAATTCCAAA+TGG - chr4_3:23589164-23589183 None:intergenic 25.0%
! AGAAAGATTAGCAAAAAACA+TGG - chr4_3:23589291-23589310 None:intergenic 25.0%
!!! TAATTGGTGGTATTTTACTA+TGG + chr4_3:23589610-23589629 Msa0635260:CDS 25.0%
! TGAGTAAACACTTAAAGATT+TGG - chr4_3:23590271-23590290 None:intergenic 25.0%
! AATAACAATAGTGTGTTTAG+CGG - chr4_3:23590400-23590419 None:intergenic 25.0%
! CTACAATTATCGTACATTAA+TGG + chr4_3:23590504-23590523 Msa0635260:intron 25.0%
! TGAGAGAAAGATTGATTATT+TGG + chr4_3:23590566-23590585 Msa0635260:intron 25.0%
! AATTTGATTACAATGAGTGT+TGG + chr4_3:23590606-23590625 Msa0635260:intron 25.0%
!! TACTAAGATTGATTCCAATT+TGG + chr4_3:23590840-23590859 Msa0635260:CDS 25.0%
!! ACTAAGATTGATTCCAATTT+GGG + chr4_3:23590841-23590860 Msa0635260:CDS 25.0%
!! TAGTCTTTTAATCTCAACAA+AGG - chr4_3:23591143-23591162 None:intergenic 25.0%
! TGTTGAGATTAAAAGACTAA+AGG + chr4_3:23591144-23591163 Msa0635260:CDS 25.0%
!! TCAAGAATTTTTCTATGATC+AGG + chr4_3:23591285-23591304 Msa0635260:CDS 25.0%
!! GTTTTGTGACAATATCAATA+AGG + chr4_3:23591435-23591454 Msa0635260:CDS 25.0%
! AAGACAACAATTTCAAAATG+GGG - chr4_3:23591709-23591728 None:intergenic 25.0%
! GAAAGACAACAATTTCAAAA+TGG - chr4_3:23591711-23591730 None:intergenic 25.0%
! GAAAAAAGGACATTTATTGA+CGG + chr4_3:23591745-23591764 Msa0635260:three_prime_UTR 25.0%
CAACACAGGTTTCTTTATTA+AGG - chr4_3:23587961-23587980 None:intergenic 30.0%
TTAATATTTAGGCACAACAC+AGG - chr4_3:23587975-23587994 None:intergenic 30.0%
! TTAGCTCTTGTTGATTACAA+AGG + chr4_3:23588092-23588111 Msa0635260:CDS 30.0%
AACTTGATATCGTATCTAAC+AGG + chr4_3:23588437-23588456 Msa0635260:CDS 30.0%
CTGCAAAGAATGTCAATATT+TGG + chr4_3:23588486-23588505 Msa0635260:CDS 30.0%
AAGAATGTCAATATTTGGTC+CGG + chr4_3:23588491-23588510 Msa0635260:CDS 30.0%
! TCATTTCTTTTATGCTTTCG+AGG + chr4_3:23588948-23588967 Msa0635260:intron 30.0%
!! AATCTGAAAGTATCTTGAAG+GGG + chr4_3:23589056-23589075 Msa0635260:intron 30.0%
AATACTGCAAATACTTGAAC+AGG - chr4_3:23589209-23589228 None:intergenic 30.0%
GGTAGGATATGTAGAATAAA+GGG - chr4_3:23589260-23589279 None:intergenic 30.0%
TGGTAGGATATGTAGAATAA+AGG - chr4_3:23589261-23589280 None:intergenic 30.0%
GTCATTGGATACAAATTACA+GGG + chr4_3:23589389-23589408 Msa0635260:intron 30.0%
AATAAGATTACTTGTGACTG+AGG - chr4_3:23589467-23589486 None:intergenic 30.0%
CTAGAAGCTCATTCTTTAAT+TGG + chr4_3:23589594-23589613 Msa0635260:CDS 30.0%
!!! TTGTTTTTTTGCTTGGAACA+AGG + chr4_3:23589738-23589757 Msa0635260:CDS 30.0%
GACATATAGGTTTAGCATTA+AGG + chr4_3:23589760-23589779 Msa0635260:CDS 30.0%
TTGTTGCAGCTATAAGAAAT+TGG + chr4_3:23589822-23589841 Msa0635260:CDS 30.0%
GCGAAATAACTTATCAACAA+CGG + chr4_3:23589844-23589863 Msa0635260:CDS 30.0%
! TAACTTATCAACAACGGATT+TGG + chr4_3:23589850-23589869 Msa0635260:CDS 30.0%
TTCCACAAGGATAAAATCAT+TGG + chr4_3:23590178-23590197 Msa0635260:intron 30.0%
! AACCAATGATTTTATCCTTG+TGG - chr4_3:23590183-23590202 None:intergenic 30.0%
TTCAGAGAAGAATAAAGACT+GGG - chr4_3:23590307-23590326 None:intergenic 30.0%
TTTCAGAGAAGAATAAAGAC+TGG - chr4_3:23590308-23590327 None:intergenic 30.0%
! ACTCCCTTCTTATTTAATCA+CGG + chr4_3:23590444-23590463 Msa0635260:intron 30.0%
!!! AGATTATGGCTATTTTGACT+TGG + chr4_3:23590709-23590728 Msa0635260:intron 30.0%
ATGAAGAGAACAATATTTCC+TGG + chr4_3:23590932-23590951 Msa0635260:CDS 30.0%
TTTATAGTTTGAAGAGATGC+AGG - chr4_3:23590971-23590990 None:intergenic 30.0%
GCATCTCTTCAAACTATAAA+CGG + chr4_3:23590971-23590990 Msa0635260:CDS 30.0%
ATCACAATGCTTCAAAGAAT+TGG + chr4_3:23591076-23591095 Msa0635260:CDS 30.0%
!! GTTCCTCAATACTTTTTGTT+TGG + chr4_3:23591232-23591251 Msa0635260:CDS 30.0%
!! ACTCCAAACAAAAAGTATTG+AGG - chr4_3:23591238-23591257 None:intergenic 30.0%
!!! AGTTTCTGAGGTTTTTACTA+TGG + chr4_3:23591255-23591274 Msa0635260:CDS 30.0%
GAAAATATTAGTGGCAAAGA+TGG + chr4_3:23591400-23591419 Msa0635260:CDS 30.0%
!! TATTTTTATTGGCTACTTGC+TGG + chr4_3:23591469-23591488 Msa0635260:CDS 30.0%
CACAGGTTTCTTTATTAAGG+TGG - chr4_3:23587958-23587977 None:intergenic 35.0%
TTAGATCCGAATCTGGTTAT+TGG + chr4_3:23588126-23588145 Msa0635260:CDS 35.0%
TGTACCTATGATAAACCAAG+CGG - chr4_3:23588156-23588175 None:intergenic 35.0%
! AAGGTTGGTTCAATAAGAGT+TGG + chr4_3:23588209-23588228 Msa0635260:intron 35.0%
! GATTTGGTAGGTCTTAACAT+CGG - chr4_3:23588272-23588291 None:intergenic 35.0%
!!! ATGCATGTTTTGTTTAGGTG+TGG + chr4_3:23588376-23588395 Msa0635260:intron 35.0%
!!! TTCGTTGCTGATTCTTTTCT+TGG + chr4_3:23588545-23588564 Msa0635260:CDS 35.0%
!! GGCGTAAAAATTCAAGTTGT+TGG - chr4_3:23588682-23588701 None:intergenic 35.0%
! AGGAGAAATTTTCGGTATCA+TGG + chr4_3:23588721-23588740 Msa0635260:intron 35.0%
! ATTTTCGGTATCATGGAGTT+TGG + chr4_3:23588728-23588747 Msa0635260:intron 35.0%
GATGCAAACTAGATGCAAAT+GGG - chr4_3:23589094-23589113 None:intergenic 35.0%
AGATGCAAACTAGATGCAAA+TGG - chr4_3:23589095-23589114 None:intergenic 35.0%
! CTAGTTTGCATCTAAACTAG+TGG + chr4_3:23589102-23589121 Msa0635260:intron 35.0%
CTAGTATGAGATCTTGTCTT+GGG - chr4_3:23589234-23589253 None:intergenic 35.0%
ACTAGTATGAGATCTTGTCT+TGG - chr4_3:23589235-23589254 None:intergenic 35.0%
!!! CATGTTTTGATGTTGGTCAT+TGG + chr4_3:23589374-23589393 Msa0635260:intron 35.0%
GGTCATTGGATACAAATTAC+AGG + chr4_3:23589388-23589407 Msa0635260:intron 35.0%
GAGAACAATATGTAAGGGAA+GGG - chr4_3:23589445-23589464 None:intergenic 35.0%
CTGAGGAGAACAATATGTAA+GGG - chr4_3:23589450-23589469 None:intergenic 35.0%
ACTGAGGAGAACAATATGTA+AGG - chr4_3:23589451-23589470 None:intergenic 35.0%
AAACCTTGTGTTCAAGCTTT+CGG + chr4_3:23589530-23589549 Msa0635260:CDS 35.0%
GAAGCTCATTCTTTAATTGG+TGG + chr4_3:23589597-23589616 Msa0635260:CDS 35.0%
! TGGTATTTTACTATGGTTGC+AGG + chr4_3:23589617-23589636 Msa0635260:CDS 35.0%
GATAACATTAGTTGGGTTCT+TGG + chr4_3:23589677-23589696 Msa0635260:CDS 35.0%
ATTAGTTGGGTTCTTGGATT+TGG + chr4_3:23589683-23589702 Msa0635260:CDS 35.0%
CAATGATCATTACGACACAA+GGG - chr4_3:23589712-23589731 None:intergenic 35.0%
TGTCGTAATGATCATTGCTT+TGG + chr4_3:23589715-23589734 Msa0635260:CDS 35.0%
!!! GCTTTGGTTGTTTTTTTGCT+TGG + chr4_3:23589731-23589750 Msa0635260:CDS 35.0%
AAGAGTCCCTTTCTTAGAAT+TGG + chr4_3:23589791-23589810 Msa0635260:CDS 35.0%
! ACACGACCAATTCTAAGAAA+GGG - chr4_3:23589800-23589819 None:intergenic 35.0%
! TACACGACCAATTCTAAGAA+AGG - chr4_3:23589801-23589820 None:intergenic 35.0%
GATTTGGAAGAAGAATGTGA+TGG + chr4_3:23589866-23589885 Msa0635260:CDS 35.0%
ACAAGCTCAAGATTCTATCA+TGG - chr4_3:23590149-23590168 None:intergenic 35.0%
! GATTTTATCCTTGTGGAATG+TGG - chr4_3:23590176-23590195 None:intergenic 35.0%
CCACCGTGATTAAATAAGAA+GGG - chr4_3:23590450-23590469 None:intergenic 35.0%
ACCACCGTGATTAAATAAGA+AGG - chr4_3:23590451-23590470 None:intergenic 35.0%
GTACATTAATGGTTCATGCT+AGG + chr4_3:23590515-23590534 Msa0635260:intron 35.0%
TCATCTTCTTTGGATCCATT+TGG - chr4_3:23590782-23590801 None:intergenic 35.0%
ACTAAAGTTGTAGCCCAAAT+TGG - chr4_3:23590857-23590876 None:intergenic 35.0%
! TGGGCTACAACTTTAGTTTA+TGG + chr4_3:23590860-23590879 Msa0635260:CDS 35.0%
TTTACATTCTTCACCAAGCA+AGG + chr4_3:23590902-23590921 Msa0635260:CDS 35.0%
TTACATTCTTCACCAAGCAA+GGG + chr4_3:23590903-23590922 Msa0635260:CDS 35.0%
CAACTGTTCCAATGAGTATA+TGG + chr4_3:23591203-23591222 Msa0635260:CDS 35.0%
!! CTTTTTGTTTGGAGTTTCTG+AGG + chr4_3:23591243-23591262 Msa0635260:CDS 35.0%
!! TCTGAGGTTTTTACTATGGT+TGG + chr4_3:23591259-23591278 Msa0635260:CDS 35.0%
CCATGCTTCTTAGTTCATAT+GGG - chr4_3:23591312-23591331 None:intergenic 35.0%
! CCATATGAACTAAGAAGCAT+GGG + chr4_3:23591310-23591329 Msa0635260:CDS 35.0%
! GCACTCTATTTGAGTATTGT+TGG + chr4_3:23591337-23591356 Msa0635260:CDS 35.0%
!! CATAAAAGTGTCATCACACA+AGG + chr4_3:23591550-23591569 Msa0635260:CDS 35.0%
GCATGCAAATGCTCTATATT+TGG + chr4_3:23591668-23591687 Msa0635260:three_prime_UTR 35.0%
GGATCTCCAATAACCAGATT+CGG - chr4_3:23588135-23588154 None:intergenic 40.0%
AGATCCGCTTGGTTTATCAT+AGG + chr4_3:23588149-23588168 Msa0635260:CDS 40.0%
AAGTTCAATCCGTGCATTTG+CGG - chr4_3:23588243-23588262 None:intergenic 40.0%
!!! GTTTTGTTTAGGTGTGGAAG+TGG + chr4_3:23588382-23588401 Msa0635260:intron 40.0%
AAAACATGGAAGACAGTGGT+AGG - chr4_3:23589277-23589296 None:intergenic 40.0%
GCAAAAAACATGGAAGACAG+TGG - chr4_3:23589281-23589300 None:intergenic 40.0%
!!! GTGTGCTCATGTTTTGATGT+TGG + chr4_3:23589367-23589386 Msa0635260:intron 40.0%
GGATACAAATTACAGGGACT+AGG + chr4_3:23589395-23589414 Msa0635260:intron 40.0%
GGAGAACAATATGTAAGGGA+AGG - chr4_3:23589446-23589465 None:intergenic 40.0%
TCACTCTATCTAGTTGCCAT+TGG + chr4_3:23589494-23589513 Msa0635260:CDS 40.0%
TATCTAGTTGCCATTGGACA+AGG + chr4_3:23589500-23589519 Msa0635260:CDS 40.0%
AACCTTGTGTTCAAGCTTTC+GGG + chr4_3:23589531-23589550 Msa0635260:CDS 40.0%
!! CTTCTAGCTTTGTGCTCTTT+AGG - chr4_3:23589581-23589600 None:intergenic 40.0%
GCAATGATCATTACGACACA+AGG - chr4_3:23589713-23589732 None:intergenic 40.0%
TGCTTGGAACAAGGACATAT+AGG + chr4_3:23589747-23589766 Msa0635260:CDS 40.0%
GCTCAAGATTCTATCATGGT+GGG - chr4_3:23590145-23590164 None:intergenic 40.0%
AGCTCAAGATTCTATCATGG+TGG - chr4_3:23590146-23590165 None:intergenic 40.0%
TTGTGATACCACATTCCACA+AGG + chr4_3:23590165-23590184 Msa0635260:intron 40.0%
AAGAAGGGAGTTGAACTATC+TGG - chr4_3:23590435-23590454 None:intergenic 40.0%
! CCCTTCTTATTTAATCACGG+TGG + chr4_3:23590447-23590466 Msa0635260:intron 40.0%
!! TTGGCTTGTAACTTCTGCAT+TGG + chr4_3:23590728-23590747 Msa0635260:intron 40.0%
!! AAGGCATTGCTAACACCAAA+TGG + chr4_3:23590764-23590783 Msa0635260:CDS 40.0%
GCTACACGTTTCATCTTCTT+TGG - chr4_3:23590792-23590811 None:intergenic 40.0%
!! TGAAACGTGTAGCTTTAGTG+AGG + chr4_3:23590799-23590818 Msa0635260:CDS 40.0%
ATAGTTTGAAGAGATGCAGG+AGG - chr4_3:23590968-23590987 None:intergenic 40.0%
CGTTATCTACGATCGCATAT+TGG + chr4_3:23591015-23591034 Msa0635260:CDS 40.0%
CCAGTTATAGATCTTGCTAG+TGG - chr4_3:23591043-23591062 None:intergenic 40.0%
CCACTAGCAAGATCTATAAC+TGG + chr4_3:23591040-23591059 Msa0635260:CDS 40.0%
TCTATAACTGGAAAACCCTC+AGG + chr4_3:23591052-23591071 Msa0635260:CDS 40.0%
!! TGAAGCATTGTGATACCTGA+GGG - chr4_3:23591070-23591089 None:intergenic 40.0%
!! TTGAAGCATTGTGATACCTG+AGG - chr4_3:23591071-23591090 None:intergenic 40.0%
ATGCTTCAAAGAATTGGCAC+TGG + chr4_3:23591082-23591101 Msa0635260:CDS 40.0%
CTCATTGGAACAGTTGCAAT+TGG - chr4_3:23591199-23591218 None:intergenic 40.0%
CTGTTCCAATGAGTATATGG+TGG + chr4_3:23591206-23591225 Msa0635260:CDS 40.0%
TCCAATGAGTATATGGTGGT+TGG + chr4_3:23591210-23591229 Msa0635260:CDS 40.0%
ACCAACCACCATATACTCAT+TGG - chr4_3:23591214-23591233 None:intergenic 40.0%
CCCATATGAACTAAGAAGCA+TGG + chr4_3:23591309-23591328 Msa0635260:CDS 40.0%
CCCATGCTTCTTAGTTCATA+TGG - chr4_3:23591313-23591332 None:intergenic 40.0%
GCAAAGATGGACATGATAGT+TGG + chr4_3:23591413-23591432 Msa0635260:CDS 40.0%
! ACTTGCTGGATTAAGTGTGT+TGG + chr4_3:23591483-23591502 Msa0635260:CDS 40.0%
CTTGCTGGATTAAGTGTGTT+GGG + chr4_3:23591484-23591503 Msa0635260:CDS 40.0%
TTGCTGGATTAAGTGTGTTG+GGG + chr4_3:23591485-23591504 Msa0635260:CDS 40.0%
! ACAAGGATAGTCACCGATTA+AGG + chr4_3:23591567-23591586 Msa0635260:exon 40.0%
ACCAGATTCGGATCTAACTG+CGG - chr4_3:23588123-23588142 None:intergenic 45.0%
AGTTCAATCCGTGCATTTGC+GGG - chr4_3:23588242-23588261 None:intergenic 45.0%
TTGGTAGGTCTTAACATCGG+TGG - chr4_3:23588269-23588288 None:intergenic 45.0%
TTGTTTAGGTGTGGAAGTGG+CGG + chr4_3:23588385-23588404 Msa0635260:intron 45.0%
!! GCGGAGAGGGTATCATATTA+TGG + chr4_3:23588404-23588423 Msa0635260:CDS 45.0%
!! GTTGCAGTGCTTTGTTTGAG+TGG - chr4_3:23588464-23588483 None:intergenic 45.0%
TCGTGTCTTCACACACCATT+TGG + chr4_3:23589146-23589165 Msa0635260:intron 45.0%
TAGTTGCCATTGGACAAGGT+GGG + chr4_3:23589504-23589523 Msa0635260:CDS 45.0%
TTTATGCCCACCTTGTCCAA+TGG - chr4_3:23589513-23589532 None:intergenic 45.0%
ACCTTGTGTTCAAGCTTTCG+GGG + chr4_3:23589532-23589551 Msa0635260:CDS 45.0%
! CATTGGTAGCTTCCTCAACA+AGG + chr4_3:23590745-23590764 Msa0635260:intron 45.0%
! GTGTTAGCAATGCCTTGTTG+AGG - chr4_3:23590760-23590779 None:intergenic 45.0%
!! AACGTGTAGCTTTAGTGAGG+TGG + chr4_3:23590802-23590821 Msa0635260:CDS 45.0%
TAGTGAGGTGGAAGAAGCAA+AGG + chr4_3:23590814-23590833 Msa0635260:CDS 45.0%
CTTCATAGAACTCCCTTGCT+TGG - chr4_3:23590918-23590937 None:intergenic 45.0%
CTAAAGGTAGCACAAGAGCA+TGG + chr4_3:23591160-23591179 Msa0635260:CDS 45.0%
! GGTGTTGCAAGCTTTCTAAG+TGG + chr4_3:23591358-23591377 Msa0635260:CDS 45.0%
CCAGATTCGGATCTAACTGC+GGG - chr4_3:23588122-23588141 None:intergenic 50.0%
CTGGTTATTGGAGATCCGCT+TGG + chr4_3:23588138-23588157 Msa0635260:CDS 50.0%
GTCAATACCCCGCAAATGCA+CGG + chr4_3:23588231-23588250 Msa0635260:intron 50.0%
GTTCAATCCGTGCATTTGCG+GGG - chr4_3:23588241-23588260 None:intergenic 50.0%
AGCACCTAAGAGAGGAAGGA+GGG - chr4_3:23588525-23588544 None:intergenic 50.0%
AAGCACCTAAGAGAGGAAGG+AGG - chr4_3:23588526-23588545 None:intergenic 50.0%
!! CGAAAGCACCTAAGAGAGGA+AGG - chr4_3:23588529-23588548 None:intergenic 50.0%
!! GCAACGAAAGCACCTAAGAG+AGG - chr4_3:23588533-23588552 None:intergenic 50.0%
CTAGTTGCCATTGGACAAGG+TGG + chr4_3:23589503-23589522 Msa0635260:CDS 50.0%
TTGTGTTCAAGCTTTCGGGG+CGG + chr4_3:23589535-23589554 Msa0635260:CDS 50.0%
! GCAGGAGGTATGTCAAAACC+AGG - chr4_3:23590953-23590972 None:intergenic 50.0%
CCCGCAGTTAGATCCGAATC+TGG + chr4_3:23588119-23588138 Msa0635260:CDS 55.0%
AGGAAGGAGGGAAGCAGTTC+CGG - chr4_3:23588513-23588532 None:intergenic 55.0%
GCTTCCCTCCTTCCTCTCTT+AGG + chr4_3:23588518-23588537 Msa0635260:CDS 55.0%
GCCCCGAAAGCTTGAACACA+AGG - chr4_3:23589536-23589555 None:intergenic 55.0%
! TAGGTGTGGAAGTGGCGGAG+AGG + chr4_3:23588390-23588409 Msa0635260:intron 60.0%
! AGGTGTGGAAGTGGCGGAGA+GGG + chr4_3:23588391-23588410 Msa0635260:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 23587928 23591773 23587928 ID=Msa0635260;Name=Msa0635260
chr4_3 mRNA 23587928 23591773 23587928 ID=Msa0635260-mRNA-1;Parent=Msa0635260;Name=Msa0635260-mRNA-1;_AED=0.35;_eAED=0.35;_QI=128|1|1|1|0.75|0.6|5|161|555
chr4_3 exon 23587928 23588170 23587928 ID=Msa0635260-mRNA-1:exon:6085;Parent=Msa0635260-mRNA-1
chr4_3 exon 23588393 23588610 23588393 ID=Msa0635260-mRNA-1:exon:6086;Parent=Msa0635260-mRNA-1
chr4_3 exon 23589410 23589921 23589410 ID=Msa0635260-mRNA-1:exon:6087;Parent=Msa0635260-mRNA-1
chr4_3 exon 23590754 23591608 23590754 ID=Msa0635260-mRNA-1:exon:6088;Parent=Msa0635260-mRNA-1
chr4_3 exon 23591645 23591773 23591645 ID=Msa0635260-mRNA-1:exon:6089;Parent=Msa0635260-mRNA-1
chr4_3 five_prime_UTR 23587928 23588055 23587928 ID=Msa0635260-mRNA-1:five_prime_utr;Parent=Msa0635260-mRNA-1
chr4_3 CDS 23588056 23588170 23588056 ID=Msa0635260-mRNA-1:cds;Parent=Msa0635260-mRNA-1
chr4_3 CDS 23588393 23588610 23588393 ID=Msa0635260-mRNA-1:cds;Parent=Msa0635260-mRNA-1
chr4_3 CDS 23589410 23589921 23589410 ID=Msa0635260-mRNA-1:cds;Parent=Msa0635260-mRNA-1
chr4_3 CDS 23590754 23591576 23590754 ID=Msa0635260-mRNA-1:cds;Parent=Msa0635260-mRNA-1
chr4_3 three_prime_UTR 23591577 23591608 23591577 ID=Msa0635260-mRNA-1:three_prime_utr;Parent=Msa0635260-mRNA-1
chr4_3 three_prime_UTR 23591645 23591773 23591645 ID=Msa0635260-mRNA-1:three_prime_utr;Parent=Msa0635260-mRNA-1
Gene Sequence

>Msa0635260

ATGGAAGAATCGAACGAAGTGTTGTTAGAAGCTGAGTTAGCTCTTGTTGATTACAAAGGACAGCCCGCAGTTAGATCCGAATCTGGTTATTGGAGATCCGCTTGGTTTATCATAGGTGTGGAAGTGGCGGAGAGGGTATCATATTATGGAATTCAAGGGAACTTGATATCGTATCTAACAGGACCACTCAAACAAAGCACTGCAACAGCTGCAAAGAATGTCAATATTTGGTCCGGAACTGCTTCCCTCCTTCCTCTCTTAGGTGCTTTCGTTGCTGATTCTTTTCTTGGACGCTATCGAACAATCATACTTGCTTCTCTTATCTATATATTGGGACTAGGCTTGTTAACACTATCAGCTTTGCTCCCTTCCCTTACATATTGTTCTCCTCAGTCACAAGTAATCTTATTCTTCGTCTCACTCTATCTAGTTGCCATTGGACAAGGTGGGCATAAACCTTGTGTTCAAGCTTTCGGGGCGGATCAATTCGATGAAAAACATCCTAAAGAGCACAAAGCTAGAAGCTCATTCTTTAATTGGTGGTATTTTACTATGGTTGCAGGTTGCACAGCAACACTTACGATCTTGAACTATATACAAGATAACATTAGTTGGGTTCTTGGATTTGGAATCCCTTGTGTCGTAATGATCATTGCTTTGGTTGTTTTTTTGCTTGGAACAAGGACATATAGGTTTAGCATTAAGGATAATGACAAGAGTCCCTTTCTTAGAATTGGTCGTGTATTTGTTGCAGCTATAAGAAATTGGCGAAATAACTTATCAACAACGGATTTGGAAGAAGAATGTGATGGACTACTTTGTCGTCAAAGTTCTGAACAATTCAACTTCCTCAACAAGGCATTGCTAACACCAAATGGATCCAAAGAAGATGAAACGTGTAGCTTTAGTGAGGTGGAAGAAGCAAAGGCAGTACTAAGATTGATTCCAATTTGGGCTACAACTTTAGTTTATGGTATTATCTTTGCTCAAATATTTACATTCTTCACCAAGCAAGGGAGTTCTATGAAGAGAACAATATTTCCTGGTTTTGACATACCTCCTGCATCTCTTCAAACTATAAACGGTGTTGCAATAGTTATCTTCAGCGTTATCTACGATCGCATATTGGTGCCACTAGCAAGATCTATAACTGGAAAACCCTCAGGTATCACAATGCTTCAAAGAATTGGCACTGGAATTTTTCTATCTATATTTACGGTAGTAATTGCAGCCTTTGTTGAGATTAAAAGACTAAAGGTAGCACAAGAGCATGGTCTTGTTGATGATCCAATTGCAACTGTTCCAATGAGTATATGGTGGTTGGTTCCTCAATACTTTTTGTTTGGAGTTTCTGAGGTTTTTACTATGGTTGGTCTTCAAGAATTTTTCTATGATCAGGTCCCATATGAACTAAGAAGCATGGGACTTGCACTCTATTTGAGTATTGTTGGTGTTGCAAGCTTTCTAAGTGGCTTTCTCATATCTTTGATAGAAAATATTAGTGGCAAAGATGGACATGATAGTTGGTTTTGTGACAATATCAATAAGGCACATCTTGATTATTTTTATTGGCTACTTGCTGGATTAAGTGTGTTGGGGTTTACTCTGTTTGTTTACTTTGCAAAATCTTACATTTATAATCATAAAAGTGTCATCACACAAGGATAG
Protein sequence

>Msa0635260

MEESNEVLLEAELALVDYKGQPAVRSESGYWRSAWFIIGVEVAERVSYYGIQGNLISYLTGPLKQSTATAAKNVNIWSGTASLLPLLGAFVADSFLGRYRTIILASLIYILGLGLLTLSALLPSLTYCSPQSQVILFFVSLYLVAIGQGGHKPCVQAFGADQFDEKHPKEHKARSSFFNWWYFTMVAGCTATLTILNYIQDNISWVLGFGIPCVVMIIALVVFLLGTRTYRFSIKDNDKSPFLRIGRVFVAAIRNWRNNLSTTDLEEECDGLLCRQSSEQFNFLNKALLTPNGSKEDETCSFSEVEEAKAVLRLIPIWATTLVYGIIFAQIFTFFTKQGSSMKRTIFPGFDIPPASLQTINGVAIVIFSVIYDRILVPLARSITGKPSGITMLQRIGTGIFLSIFTVVIAAFVEIKRLKVAQEHGLVDDPIATVPMSIWWLVPQYFLFGVSEVFTMVGLQEFFYDQVPYELRSMGLALYLSIVGVASFLSGFLISLIENISGKDGHDSWFCDNINKAHLDYFYWLLAGLSVLGFTLFVYFAKSYIYNHKSVITQG*