AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0642540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0642540 MtrunA17_Chr4g0028161 87.109 256 14 4 1 240 1 253 1.24e-142 399
Msa0642540 MtrunA17_Chr2g0320271 59.470 264 55 11 1 238 1 238 2.02e-92 271
Msa0642540 MtrunA17_Chr1g0197151 44.311 167 67 3 15 181 29 169 1.08e-34 124
Msa0642540 MtrunA17_Chr4g0030841 36.842 190 68 7 36 186 29 205 1.09e-20 88.2
Msa0642540 MtrunA17_Chr2g0327801 32.222 180 95 5 29 186 30 204 3.23e-18 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0642540 AT5G22930.1 50.570 263 80 8 1 239 1 237 3.73e-73 223
Msa0642540 AT3G44940.1 48.315 267 88 9 5 240 3 250 3.07e-56 180
Msa0642540 AT2G28140.1 41.154 260 81 8 1 238 1 210 7.85e-47 155
Msa0642540 AT2G28690.1 33.140 172 112 2 15 185 23 192 5.19e-23 94.0
Msa0642540 AT5G59760.1 31.765 170 107 4 19 186 28 190 2.19e-15 73.2

Find 62 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATTCAATAGTAGAAAA+AGG 0.231209 4_3:+44294935 Msa0642540:CDS
GATAAACCTTTGCCAGAAAA+AGG 0.289825 4_3:+44294709 Msa0642540:CDS
AGAGCATAGGGGAGGGAGTT+AGG 0.342485 4_3:+44293762 Msa0642540:five_prime_UTR
GGAGTCCAAGTAACTGTATA+AGG 0.351534 4_3:-44293857 None:intergenic
GTCCATGTTCTTAGAAAGAA+TGG 0.356244 4_3:-44293986 None:intergenic
AGAAATGAGAAAAGAAGAAA+AGG 0.362600 4_3:-44293941 None:intergenic
AACGTACCCCTCTTCTATCT+TGG 0.371913 4_3:+44294016 Msa0642540:CDS
GAAATGAGAAAAGAAGAAAA+GGG 0.376470 4_3:-44293940 None:intergenic
TGGGAGAGAGACAGAGTTGT+GGG 0.399773 4_3:-44293966 None:intergenic
TGATAGTGATATTCCATTGT+TGG 0.406170 4_3:-44294605 None:intergenic
AGGAAGGTTGCCTTATAAAC+CGG 0.406966 4_3:-44293837 None:intergenic
ATTGAATCAATCATCATCAT+TGG 0.412861 4_3:-44294679 None:intergenic
TGCTGATGCTGTGGTGGTTG+AGG 0.414937 4_3:-44294868 None:intergenic
ACCTTCCTTATACAGTTACT+TGG 0.414957 4_3:+44293852 Msa0642540:five_prime_UTR
AGATTCCAGACAAAGGATCT+TGG 0.415576 4_3:-44294531 None:intergenic
AGAGTGTTAGGAGTGTTATG+AGG 0.442989 4_3:-44293910 None:intergenic
ACGTACCCCTCTTCTATCTT+GGG 0.445573 4_3:+44294017 Msa0642540:CDS
ATTGAAGGTGTCTTATAAGA+AGG 0.454218 4_3:-44293878 None:intergenic
ATGAGGGATGGTTGAATTGA+AGG 0.457056 4_3:-44293893 None:intergenic
GGATGTCTCCATTGAGGTAA+TGG 0.460747 4_3:-44294793 None:intergenic
GTAATGGTCCTGCAAGCAAA+AGG 0.461418 4_3:-44294777 None:intergenic
CAAAACCAAGATCCTTTGTC+TGG 0.462496 4_3:+44294526 Msa0642540:CDS
GAAGTATAGGTACCTTTCCT+TGG 0.469839 4_3:-44294050 None:intergenic
GAGTGTTAGGAGTGTTATGA+GGG 0.477852 4_3:-44293909 None:intergenic
GTCAATGGAGTACAATCCTC+TGG 0.508694 4_3:-44295002 None:intergenic
ATGGGAGAGAGACAGAGTTG+TGG 0.509113 4_3:-44293967 None:intergenic
AAAGACTCCAATGGTGGTGG+AGG 0.517761 4_3:-44294814 None:intergenic
TGGTTGAGGTGGTGAATGAA+TGG 0.519132 4_3:-44294854 None:intergenic
GAGACATCCTCCACCACCAT+TGG 0.528054 4_3:+44294807 Msa0642540:CDS
TCCCATTCTTTCTAAGAACA+TGG 0.529241 4_3:+44293984 Msa0642540:exon
TTGCAGGACCATTACCTCAA+TGG 0.529726 4_3:+44294785 Msa0642540:CDS
AAATGAGAAAAGAAGAAAAG+GGG 0.530074 4_3:-44293939 None:intergenic
GTTAGGAGTGTTATGAGGGA+TGG 0.536015 4_3:-44293905 None:intergenic
TTACAAGCAGTGATGAAAGC+TGG 0.538923 4_3:+44294739 Msa0642540:CDS
AGCAAAAGGGTCTGAAGCAA+AGG 0.539159 4_3:-44294763 None:intergenic
TCCATGTTCTTAGAAAGAAT+GGG 0.544639 4_3:-44293985 None:intergenic
TCAAAAGACTCCAATGGTGG+TGG 0.547170 4_3:-44294817 None:intergenic
GGAATTGAATCTTCCAACAA+TGG 0.550626 4_3:+44294592 Msa0642540:CDS
AGTAAGCCCAAGATAGAAGA+GGG 0.552955 4_3:-44294023 None:intergenic
TGTTGCTGCTGATGCTGTGG+TGG 0.555209 4_3:-44294874 None:intergenic
TGATGCTGTGGTGGTTGAGG+TGG 0.562813 4_3:-44294865 None:intergenic
TAGGGGAGGGAGTTAGGACT+TGG 0.564748 4_3:+44293768 Msa0642540:five_prime_UTR
TAATGGTCCTGCAAGCAAAA+GGG 0.574855 4_3:-44294776 None:intergenic
AGTTGTTGCTGCTGATGCTG+TGG 0.582168 4_3:-44294877 None:intergenic
GGAAGGTTGCCTTATAAACC+GGG 0.587789 4_3:-44293836 None:intergenic
TGGTACTTATTCTCAGCAGA+AGG 0.589538 4_3:-44294982 None:intergenic
TCCAAGTAACTGTATAAGGA+AGG 0.595114 4_3:-44293853 None:intergenic
ATGCTTGAGATTCCAGACAA+AGG 0.616707 4_3:-44294538 None:intergenic
TCTTGGGCTTACTACTGCCA+AGG 0.616963 4_3:+44294033 Msa0642540:CDS
TAGTAAGCCCAAGATAGAAG+AGG 0.618226 4_3:-44294024 None:intergenic
GGCTTACTACTGCCAAGGAA+AGG 0.619012 4_3:+44294038 Msa0642540:CDS
GGAATCTCAAAAGACTCCAA+TGG 0.630030 4_3:-44294823 None:intergenic
CTGCTGAGAATAAGTACCAG+AGG 0.635351 4_3:+44294986 Msa0642540:CDS
AAGGTTGCCTTATAAACCGG+GGG 0.643287 4_3:-44293834 None:intergenic
ATCTCAAAAGACTCCAATGG+TGG 0.646695 4_3:-44294820 None:intergenic
AACAGACAATAGGCAATCTG+TGG 0.649832 4_3:-44293791 None:intergenic
GGTGGTGAATGAATGGTCAC+AGG 0.654394 4_3:-44294847 None:intergenic
GGTGGAGGATGTCTCCATTG+AGG 0.679502 4_3:-44294799 None:intergenic
GGTGAATGAATGGTCACAGG+TGG 0.686774 4_3:-44294844 None:intergenic
GTAAGCCCAAGATAGAAGAG+GGG 0.693101 4_3:-44294022 None:intergenic
GAAGGTTGCCTTATAAACCG+GGG 0.734119 4_3:-44293835 None:intergenic
AGGTTGCCTTATAAACCGGG+GGG 0.792960 4_3:-44293833 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATTTATTAAAAAAATG+GGG - chr4_3:44294273-44294292 None:intergenic 10.0%
!! TGAAATTTATTAAAAAAATG+GGG - chr4_3:44294273-44294292 None:intergenic 10.0%
!!! TAAAGAAAGAAAAGAAGTAT+AGG - chr4_3:44294066-44294085 None:intergenic 20.0%
!!! GTTAGTCTCATAAAATTAAA+AGG - chr4_3:44294172-44294191 None:intergenic 20.0%
!! AAGATTCAATAGTAGAAAAA+GGG + chr4_3:44294936-44294955 Msa0642540:CDS 20.0%
!!! TAAAGAAAGAAAAGAAGTAT+AGG - chr4_3:44294066-44294085 None:intergenic 20.0%
!!! GTTAGTCTCATAAAATTAAA+AGG - chr4_3:44294172-44294191 None:intergenic 20.0%
!! AAGATTCAATAGTAGAAAAA+GGG + chr4_3:44294936-44294955 Msa0642540:CDS 20.0%
! AAAACAGAAAAACAGACAAT+AGG - chr4_3:44293804-44293823 None:intergenic 25.0%
! AAATGAGAAAAGAAGAAAAG+GGG - chr4_3:44293942-44293961 None:intergenic 25.0%
! GAAATGAGAAAAGAAGAAAA+GGG - chr4_3:44293943-44293962 None:intergenic 25.0%
! AGAAATGAGAAAAGAAGAAA+AGG - chr4_3:44293944-44293963 None:intergenic 25.0%
! ATTCTTCATAGTTACATTTG+AGG + chr4_3:44294095-44294114 Msa0642540:intron 25.0%
!! AAACATTTTGGATGAATAGT+TGG - chr4_3:44294122-44294141 None:intergenic 25.0%
!!! AGTTAGTCTTGTAAACATTT+TGG - chr4_3:44294134-44294153 None:intergenic 25.0%
!! CACTAATGATCATTTTACAA+CGG - chr4_3:44294215-44294234 None:intergenic 25.0%
!! AATTTTTGTGATACAGTCAA+TGG + chr4_3:44294297-44294316 Msa0642540:intron 25.0%
!! CTTTGTTGAAAAGTTCTTTT+AGG - chr4_3:44294423-44294442 None:intergenic 25.0%
!!! ACTTTTCTAAGAAAAGTCTT+TGG - chr4_3:44294480-44294499 None:intergenic 25.0%
!!! TTTGTTGTTGTTTTTGTTGT+TGG - chr4_3:44294510-44294529 None:intergenic 25.0%
! ATTGAATCAATCATCATCAT+TGG - chr4_3:44294682-44294701 None:intergenic 25.0%
!! TGTAAAAGTTTACCTTTTTC+TGG - chr4_3:44294724-44294743 None:intergenic 25.0%
! GAAGATTCAATAGTAGAAAA+AGG + chr4_3:44294935-44294954 Msa0642540:CDS 25.0%
! AAAACAGAAAAACAGACAAT+AGG - chr4_3:44293804-44293823 None:intergenic 25.0%
! AAATGAGAAAAGAAGAAAAG+GGG - chr4_3:44293942-44293961 None:intergenic 25.0%
! GAAATGAGAAAAGAAGAAAA+GGG - chr4_3:44293943-44293962 None:intergenic 25.0%
! AGAAATGAGAAAAGAAGAAA+AGG - chr4_3:44293944-44293963 None:intergenic 25.0%
! ATTCTTCATAGTTACATTTG+AGG + chr4_3:44294095-44294114 Msa0642540:intron 25.0%
!! AAACATTTTGGATGAATAGT+TGG - chr4_3:44294122-44294141 None:intergenic 25.0%
!!! AGTTAGTCTTGTAAACATTT+TGG - chr4_3:44294134-44294153 None:intergenic 25.0%
!! CACTAATGATCATTTTACAA+CGG - chr4_3:44294215-44294234 None:intergenic 25.0%
!! AATTTTTGTGATACAGTCAA+TGG + chr4_3:44294297-44294316 Msa0642540:intron 25.0%
!! CTTTGTTGAAAAGTTCTTTT+AGG - chr4_3:44294423-44294442 None:intergenic 25.0%
!!! ACTTTTCTAAGAAAAGTCTT+TGG - chr4_3:44294480-44294499 None:intergenic 25.0%
!!! TTTGTTGTTGTTTTTGTTGT+TGG - chr4_3:44294510-44294529 None:intergenic 25.0%
! ATTGAATCAATCATCATCAT+TGG - chr4_3:44294682-44294701 None:intergenic 25.0%
!! TGTAAAAGTTTACCTTTTTC+TGG - chr4_3:44294724-44294743 None:intergenic 25.0%
! GAAGATTCAATAGTAGAAAA+AGG + chr4_3:44294935-44294954 Msa0642540:CDS 25.0%
! AATAGGATTTTGTAGAGCAT+AGG + chr4_3:44293749-44293768 Msa0642540:five_prime_UTR 30.0%
! ATAGGATTTTGTAGAGCATA+GGG + chr4_3:44293750-44293769 Msa0642540:five_prime_UTR 30.0%
!! ATTGAAGGTGTCTTATAAGA+AGG - chr4_3:44293881-44293900 None:intergenic 30.0%
TCCATGTTCTTAGAAAGAAT+GGG - chr4_3:44293988-44294007 None:intergenic 30.0%
! TGATAGTGATATTCCATTGT+TGG - chr4_3:44294608-44294627 None:intergenic 30.0%
!!! TTCTGTTTTTGTTTGTTGAG+GGG - chr4_3:44294656-44294675 None:intergenic 30.0%
!!! GTTCTGTTTTTGTTTGTTGA+GGG - chr4_3:44294657-44294676 None:intergenic 30.0%
!!! TGTTCTGTTTTTGTTTGTTG+AGG - chr4_3:44294658-44294677 None:intergenic 30.0%
! TCTTTTGTTAACAGTAACTG+TGG + chr4_3:44294913-44294932 Msa0642540:CDS 30.0%
!!! AGAAAAAGGGTTTTTTGTGA+AGG + chr4_3:44294949-44294968 Msa0642540:CDS 30.0%
! AATAGGATTTTGTAGAGCAT+AGG + chr4_3:44293749-44293768 Msa0642540:five_prime_UTR 30.0%
! ATAGGATTTTGTAGAGCATA+GGG + chr4_3:44293750-44293769 Msa0642540:five_prime_UTR 30.0%
!! ATTGAAGGTGTCTTATAAGA+AGG - chr4_3:44293881-44293900 None:intergenic 30.0%
TCCATGTTCTTAGAAAGAAT+GGG - chr4_3:44293988-44294007 None:intergenic 30.0%
! TGATAGTGATATTCCATTGT+TGG - chr4_3:44294608-44294627 None:intergenic 30.0%
!!! TTCTGTTTTTGTTTGTTGAG+GGG - chr4_3:44294656-44294675 None:intergenic 30.0%
!!! GTTCTGTTTTTGTTTGTTGA+GGG - chr4_3:44294657-44294676 None:intergenic 30.0%
!!! TGTTCTGTTTTTGTTTGTTG+AGG - chr4_3:44294658-44294677 None:intergenic 30.0%
! TCTTTTGTTAACAGTAACTG+TGG + chr4_3:44294913-44294932 Msa0642540:CDS 30.0%
!!! AGAAAAAGGGTTTTTTGTGA+AGG + chr4_3:44294949-44294968 Msa0642540:CDS 30.0%
! TAGGATTTTGTAGAGCATAG+GGG + chr4_3:44293751-44293770 Msa0642540:five_prime_UTR 35.0%
ACCTTCCTTATACAGTTACT+TGG + chr4_3:44293852-44293871 Msa0642540:five_prime_UTR 35.0%
TCCAAGTAACTGTATAAGGA+AGG - chr4_3:44293856-44293875 None:intergenic 35.0%
! TCCCATTCTTTCTAAGAACA+TGG + chr4_3:44293984-44294003 Msa0642540:exon 35.0%
GTCCATGTTCTTAGAAAGAA+TGG - chr4_3:44293989-44294008 None:intergenic 35.0%
AACCTTGATGTACACAACAA+TGG + chr4_3:44294333-44294352 Msa0642540:intron 35.0%
CAACAACAACAACAAAAGAG+AGG + chr4_3:44294571-44294590 Msa0642540:CDS 35.0%
GGAATTGAATCTTCCAACAA+TGG + chr4_3:44294592-44294611 Msa0642540:CDS 35.0%
GATAAACCTTTGCCAGAAAA+AGG + chr4_3:44294709-44294728 Msa0642540:CDS 35.0%
!! AGTTTACCTTTTTCTGGCAA+AGG - chr4_3:44294718-44294737 None:intergenic 35.0%
! TAGGATTTTGTAGAGCATAG+GGG + chr4_3:44293751-44293770 Msa0642540:five_prime_UTR 35.0%
ACCTTCCTTATACAGTTACT+TGG + chr4_3:44293852-44293871 Msa0642540:five_prime_UTR 35.0%
TCCAAGTAACTGTATAAGGA+AGG - chr4_3:44293856-44293875 None:intergenic 35.0%
! TCCCATTCTTTCTAAGAACA+TGG + chr4_3:44293984-44294003 Msa0642540:exon 35.0%
GTCCATGTTCTTAGAAAGAA+TGG - chr4_3:44293989-44294008 None:intergenic 35.0%
AACCTTGATGTACACAACAA+TGG + chr4_3:44294333-44294352 Msa0642540:intron 35.0%
CAACAACAACAACAAAAGAG+AGG + chr4_3:44294571-44294590 Msa0642540:CDS 35.0%
GGAATTGAATCTTCCAACAA+TGG + chr4_3:44294592-44294611 Msa0642540:CDS 35.0%
GATAAACCTTTGCCAGAAAA+AGG + chr4_3:44294709-44294728 Msa0642540:CDS 35.0%
!! AGTTTACCTTTTTCTGGCAA+AGG - chr4_3:44294718-44294737 None:intergenic 35.0%
! ATTTTGTAGAGCATAGGGGA+GGG + chr4_3:44293755-44293774 Msa0642540:five_prime_UTR 40.0%
AACAGACAATAGGCAATCTG+TGG - chr4_3:44293794-44293813 None:intergenic 40.0%
AGGAAGGTTGCCTTATAAAC+CGG - chr4_3:44293840-44293859 None:intergenic 40.0%
GGAGTCCAAGTAACTGTATA+AGG - chr4_3:44293860-44293879 None:intergenic 40.0%
ATGAGGGATGGTTGAATTGA+AGG - chr4_3:44293896-44293915 None:intergenic 40.0%
!! GAGTGTTAGGAGTGTTATGA+GGG - chr4_3:44293912-44293931 None:intergenic 40.0%
!! AGAGTGTTAGGAGTGTTATG+AGG - chr4_3:44293913-44293932 None:intergenic 40.0%
!! AAGGGGAGAAAAAGAGTGTT+AGG - chr4_3:44293925-44293944 None:intergenic 40.0%
AGTAAGCCCAAGATAGAAGA+GGG - chr4_3:44294026-44294045 None:intergenic 40.0%
TAGTAAGCCCAAGATAGAAG+AGG - chr4_3:44294027-44294046 None:intergenic 40.0%
GAAGTATAGGTACCTTTCCT+TGG - chr4_3:44294053-44294072 None:intergenic 40.0%
TTTACAACGGTTCTTCTGAC+AGG - chr4_3:44294202-44294221 None:intergenic 40.0%
CTCCATTGTTGTGTACATCA+AGG - chr4_3:44294338-44294357 None:intergenic 40.0%
CAAAACCAAGATCCTTTGTC+TGG + chr4_3:44294526-44294545 Msa0642540:CDS 40.0%
AGATTCCAGACAAAGGATCT+TGG - chr4_3:44294534-44294553 None:intergenic 40.0%
ATGCTTGAGATTCCAGACAA+AGG - chr4_3:44294541-44294560 None:intergenic 40.0%
!! TGTTTTTGTTTGTTGAGGGG+AGG - chr4_3:44294653-44294672 None:intergenic 40.0%
TTACAAGCAGTGATGAAAGC+TGG + chr4_3:44294739-44294758 Msa0642540:CDS 40.0%
TAATGGTCCTGCAAGCAAAA+GGG - chr4_3:44294779-44294798 None:intergenic 40.0%
ATCTCAAAAGACTCCAATGG+TGG - chr4_3:44294823-44294842 None:intergenic 40.0%
GGAATCTCAAAAGACTCCAA+TGG - chr4_3:44294826-44294845 None:intergenic 40.0%
TGGTACTTATTCTCAGCAGA+AGG - chr4_3:44294985-44295004 None:intergenic 40.0%
! ATTTTGTAGAGCATAGGGGA+GGG + chr4_3:44293755-44293774 Msa0642540:five_prime_UTR 40.0%
AACAGACAATAGGCAATCTG+TGG - chr4_3:44293794-44293813 None:intergenic 40.0%
AGGAAGGTTGCCTTATAAAC+CGG - chr4_3:44293840-44293859 None:intergenic 40.0%
GGAGTCCAAGTAACTGTATA+AGG - chr4_3:44293860-44293879 None:intergenic 40.0%
ATGAGGGATGGTTGAATTGA+AGG - chr4_3:44293896-44293915 None:intergenic 40.0%
!! GAGTGTTAGGAGTGTTATGA+GGG - chr4_3:44293912-44293931 None:intergenic 40.0%
!! AGAGTGTTAGGAGTGTTATG+AGG - chr4_3:44293913-44293932 None:intergenic 40.0%
!! AAGGGGAGAAAAAGAGTGTT+AGG - chr4_3:44293925-44293944 None:intergenic 40.0%
AGTAAGCCCAAGATAGAAGA+GGG - chr4_3:44294026-44294045 None:intergenic 40.0%
TAGTAAGCCCAAGATAGAAG+AGG - chr4_3:44294027-44294046 None:intergenic 40.0%
GAAGTATAGGTACCTTTCCT+TGG - chr4_3:44294053-44294072 None:intergenic 40.0%
TTTACAACGGTTCTTCTGAC+AGG - chr4_3:44294202-44294221 None:intergenic 40.0%
CTCCATTGTTGTGTACATCA+AGG - chr4_3:44294338-44294357 None:intergenic 40.0%
CAAAACCAAGATCCTTTGTC+TGG + chr4_3:44294526-44294545 Msa0642540:CDS 40.0%
AGATTCCAGACAAAGGATCT+TGG - chr4_3:44294534-44294553 None:intergenic 40.0%
ATGCTTGAGATTCCAGACAA+AGG - chr4_3:44294541-44294560 None:intergenic 40.0%
!! TGTTTTTGTTTGTTGAGGGG+AGG - chr4_3:44294653-44294672 None:intergenic 40.0%
TTACAAGCAGTGATGAAAGC+TGG + chr4_3:44294739-44294758 Msa0642540:CDS 40.0%
TAATGGTCCTGCAAGCAAAA+GGG - chr4_3:44294779-44294798 None:intergenic 40.0%
ATCTCAAAAGACTCCAATGG+TGG - chr4_3:44294823-44294842 None:intergenic 40.0%
GGAATCTCAAAAGACTCCAA+TGG - chr4_3:44294826-44294845 None:intergenic 40.0%
TGGTACTTATTCTCAGCAGA+AGG - chr4_3:44294985-44295004 None:intergenic 40.0%
! GATTTTGTAGAGCATAGGGG+AGG + chr4_3:44293754-44293773 Msa0642540:five_prime_UTR 45.0%
!!! TGTTTTTTTCTCCAACCCCC+CGG + chr4_3:44293818-44293837 Msa0642540:five_prime_UTR 45.0%
AAGGTTGCCTTATAAACCGG+GGG - chr4_3:44293837-44293856 None:intergenic 45.0%
GAAGGTTGCCTTATAAACCG+GGG - chr4_3:44293838-44293857 None:intergenic 45.0%
GGAAGGTTGCCTTATAAACC+GGG - chr4_3:44293839-44293858 None:intergenic 45.0%
!! GTTAGGAGTGTTATGAGGGA+TGG - chr4_3:44293908-44293927 None:intergenic 45.0%
AACGTACCCCTCTTCTATCT+TGG + chr4_3:44294016-44294035 Msa0642540:CDS 45.0%
ACGTACCCCTCTTCTATCTT+GGG + chr4_3:44294017-44294036 Msa0642540:CDS 45.0%
GTAAGCCCAAGATAGAAGAG+GGG - chr4_3:44294025-44294044 None:intergenic 45.0%
! AGCAAAAGGGTCTGAAGCAA+AGG - chr4_3:44294766-44294785 None:intergenic 45.0%
GTAATGGTCCTGCAAGCAAA+AGG - chr4_3:44294780-44294799 None:intergenic 45.0%
TTGCAGGACCATTACCTCAA+TGG + chr4_3:44294785-44294804 Msa0642540:CDS 45.0%
GGATGTCTCCATTGAGGTAA+TGG - chr4_3:44294796-44294815 None:intergenic 45.0%
TCAAAAGACTCCAATGGTGG+TGG - chr4_3:44294820-44294839 None:intergenic 45.0%
TGGTTGAGGTGGTGAATGAA+TGG - chr4_3:44294857-44294876 None:intergenic 45.0%
CTGCTGAGAATAAGTACCAG+AGG + chr4_3:44294986-44295005 Msa0642540:CDS 45.0%
! GATTTTGTAGAGCATAGGGG+AGG + chr4_3:44293754-44293773 Msa0642540:five_prime_UTR 45.0%
!!! TGTTTTTTTCTCCAACCCCC+CGG + chr4_3:44293818-44293837 Msa0642540:five_prime_UTR 45.0%
AAGGTTGCCTTATAAACCGG+GGG - chr4_3:44293837-44293856 None:intergenic 45.0%
GAAGGTTGCCTTATAAACCG+GGG - chr4_3:44293838-44293857 None:intergenic 45.0%
GGAAGGTTGCCTTATAAACC+GGG - chr4_3:44293839-44293858 None:intergenic 45.0%
!! GTTAGGAGTGTTATGAGGGA+TGG - chr4_3:44293908-44293927 None:intergenic 45.0%
AACGTACCCCTCTTCTATCT+TGG + chr4_3:44294016-44294035 Msa0642540:CDS 45.0%
ACGTACCCCTCTTCTATCTT+GGG + chr4_3:44294017-44294036 Msa0642540:CDS 45.0%
GTAAGCCCAAGATAGAAGAG+GGG - chr4_3:44294025-44294044 None:intergenic 45.0%
! AGCAAAAGGGTCTGAAGCAA+AGG - chr4_3:44294766-44294785 None:intergenic 45.0%
GTAATGGTCCTGCAAGCAAA+AGG - chr4_3:44294780-44294799 None:intergenic 45.0%
TTGCAGGACCATTACCTCAA+TGG + chr4_3:44294785-44294804 Msa0642540:CDS 45.0%
GGATGTCTCCATTGAGGTAA+TGG - chr4_3:44294796-44294815 None:intergenic 45.0%
TCAAAAGACTCCAATGGTGG+TGG - chr4_3:44294820-44294839 None:intergenic 45.0%
TGGTTGAGGTGGTGAATGAA+TGG - chr4_3:44294857-44294876 None:intergenic 45.0%
CTGCTGAGAATAAGTACCAG+AGG + chr4_3:44294986-44295005 Msa0642540:CDS 45.0%
!!! TTTTTAATAAATAATACTAA+TGG + chr4_3:44294237-44294256 Msa0642540:intron 5.0%
!! ATGAAATTTATTAAAAAAAT+GGG - chr4_3:44294274-44294293 None:intergenic 5.0%
!! AATGAAATTTATTAAAAAAA+TGG - chr4_3:44294275-44294294 None:intergenic 5.0%
!!! TTTTTAATAAATAATACTAA+TGG + chr4_3:44294237-44294256 Msa0642540:intron 5.0%
!! ATGAAATTTATTAAAAAAAT+GGG - chr4_3:44294274-44294293 None:intergenic 5.0%
!! AATGAAATTTATTAAAAAAA+TGG - chr4_3:44294275-44294294 None:intergenic 5.0%
AGGTTGCCTTATAAACCGGG+GGG - chr4_3:44293836-44293855 None:intergenic 50.0%
TGGGAGAGAGACAGAGTTGT+GGG - chr4_3:44293969-44293988 None:intergenic 50.0%
ATGGGAGAGAGACAGAGTTG+TGG - chr4_3:44293970-44293989 None:intergenic 50.0%
TCTTGGGCTTACTACTGCCA+AGG + chr4_3:44294033-44294052 Msa0642540:CDS 50.0%
GGCTTACTACTGCCAAGGAA+AGG + chr4_3:44294038-44294057 Msa0642540:CDS 50.0%
! CTTCAGACCCTTTTGCTTGC+AGG + chr4_3:44294769-44294788 Msa0642540:CDS 50.0%
!! AAAGACTCCAATGGTGGTGG+AGG - chr4_3:44294817-44294836 None:intergenic 50.0%
GGTGAATGAATGGTCACAGG+TGG - chr4_3:44294847-44294866 None:intergenic 50.0%
GGTGGTGAATGAATGGTCAC+AGG - chr4_3:44294850-44294869 None:intergenic 50.0%
AGTTGTTGCTGCTGATGCTG+TGG - chr4_3:44294880-44294899 None:intergenic 50.0%
AGGTTGCCTTATAAACCGGG+GGG - chr4_3:44293836-44293855 None:intergenic 50.0%
TGGGAGAGAGACAGAGTTGT+GGG - chr4_3:44293969-44293988 None:intergenic 50.0%
ATGGGAGAGAGACAGAGTTG+TGG - chr4_3:44293970-44293989 None:intergenic 50.0%
TCTTGGGCTTACTACTGCCA+AGG + chr4_3:44294033-44294052 Msa0642540:CDS 50.0%
GGCTTACTACTGCCAAGGAA+AGG + chr4_3:44294038-44294057 Msa0642540:CDS 50.0%
! CTTCAGACCCTTTTGCTTGC+AGG + chr4_3:44294769-44294788 Msa0642540:CDS 50.0%
!! AAAGACTCCAATGGTGGTGG+AGG - chr4_3:44294817-44294836 None:intergenic 50.0%
GGTGAATGAATGGTCACAGG+TGG - chr4_3:44294847-44294866 None:intergenic 50.0%
GGTGGTGAATGAATGGTCAC+AGG - chr4_3:44294850-44294869 None:intergenic 50.0%
AGTTGTTGCTGCTGATGCTG+TGG - chr4_3:44294880-44294899 None:intergenic 50.0%
AGAGCATAGGGGAGGGAGTT+AGG + chr4_3:44293762-44293781 Msa0642540:five_prime_UTR 55.0%
TAGGGGAGGGAGTTAGGACT+TGG + chr4_3:44293768-44293787 Msa0642540:five_prime_UTR 55.0%
CTCCAACCCCCCGGTTTATA+AGG + chr4_3:44293827-44293846 Msa0642540:five_prime_UTR 55.0%
TGCCTTATAAACCGGGGGGT+TGG - chr4_3:44293832-44293851 None:intergenic 55.0%
GGTGGAGGATGTCTCCATTG+AGG - chr4_3:44294802-44294821 None:intergenic 55.0%
GAGACATCCTCCACCACCAT+TGG + chr4_3:44294807-44294826 Msa0642540:CDS 55.0%
TGATGCTGTGGTGGTTGAGG+TGG - chr4_3:44294868-44294887 None:intergenic 55.0%
TGCTGATGCTGTGGTGGTTG+AGG - chr4_3:44294871-44294890 None:intergenic 55.0%
TGTTGCTGCTGATGCTGTGG+TGG - chr4_3:44294877-44294896 None:intergenic 55.0%
AGAGCATAGGGGAGGGAGTT+AGG + chr4_3:44293762-44293781 Msa0642540:five_prime_UTR 55.0%
TAGGGGAGGGAGTTAGGACT+TGG + chr4_3:44293768-44293787 Msa0642540:five_prime_UTR 55.0%
CTCCAACCCCCCGGTTTATA+AGG + chr4_3:44293827-44293846 Msa0642540:five_prime_UTR 55.0%
TGCCTTATAAACCGGGGGGT+TGG - chr4_3:44293832-44293851 None:intergenic 55.0%
GGTGGAGGATGTCTCCATTG+AGG - chr4_3:44294802-44294821 None:intergenic 55.0%
GAGACATCCTCCACCACCAT+TGG + chr4_3:44294807-44294826 Msa0642540:CDS 55.0%
TGATGCTGTGGTGGTTGAGG+TGG - chr4_3:44294868-44294887 None:intergenic 55.0%
TGCTGATGCTGTGGTGGTTG+AGG - chr4_3:44294871-44294890 None:intergenic 55.0%
TGTTGCTGCTGATGCTGTGG+TGG - chr4_3:44294877-44294896 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 44293748 44295023 44293748 ID=Msa0642540;Name=Msa0642540
chr4_3 mRNA 44293748 44295023 44293748 ID=Msa0642540-mRNA-1;Parent=Msa0642540;Name=Msa0642540-mRNA-1;_AED=0.20;_eAED=0.20;_QI=255|0|0.5|1|0|0.5|2|0|240
chr4_3 exon 44293748 44294059 44293748 ID=Msa0642540-mRNA-1:exon:10145;Parent=Msa0642540-mRNA-1
chr4_3 exon 44294358 44295023 44294358 ID=Msa0642540-mRNA-1:exon:10146;Parent=Msa0642540-mRNA-1
chr4_3 five_prime_UTR 44293748 44294002 44293748 ID=Msa0642540-mRNA-1:five_prime_utr;Parent=Msa0642540-mRNA-1
chr4_3 CDS 44294003 44294059 44294003 ID=Msa0642540-mRNA-1:cds;Parent=Msa0642540-mRNA-1
chr4_3 CDS 44294358 44295023 44294358 ID=Msa0642540-mRNA-1:cds;Parent=Msa0642540-mRNA-1
Gene Sequence

>Msa0642540

ATGGACAATCAACAACGTACCCCTCTTCTATCTTGGGCTTACTACTGCCAAGGAAAGCTTGAACAAACAAGAGTAACAGTACAAGAAGAACTAAGAAAAAGAGATGAACAACTACATAACCTAAAAGAACTTTTCAACAAAGTTGTACGAGAAAGAGATGAAGCACAAGAAAAATGCCAAAGACTTTTCTTAGAAAAGTTACTTTTCCAACAACAAAAACAACAACAAAACCAAGATCCTTTGTCTGGAATCTCAAGCATTGAAGATGAACAACAACAACAACAAAAGAGAGGAATTGAATCTTCCAACAATGGAATATCACTATCAGATTGTGAAGAAAGCATAGTTTCCTCCCCTCAACAAACAAAAACAGAACAACCAATGATGATGATTGATTCAATAGCAAAAGATAAACCTTTGCCAGAAAAAGGTAAACTTTTACAAGCAGTGATGAAAGCTGGTCCTTTGCTTCAGACCCTTTTGCTTGCAGGACCATTACCTCAATGGAGACATCCTCCACCACCATTGGAGTCTTTTGAGATTCCACCTGTGACCATTCATTCACCACCTCAACCACCACAGCATCAGCAGCAACAACTACTTCATCAAGAGTCTTTTGTTAACAGTAACTGTGGAAGATTCAATAGTAGAAAAAGGGTTTTTTGTGAAGGTTCTGATTCTCCTTCTGCTGAGAATAAGTACCAGAGGATTGTACTCCATTGA
Protein sequence

>Msa0642540

MDNQQRTPLLSWAYYCQGKLEQTRVTVQEELRKRDEQLHNLKELFNKVVRERDEAQEKCQRLFLEKLLFQQQKQQQNQDPLSGISSIEDEQQQQQKRGIESSNNGISLSDCEESIVSSPQQTKTEQPMMMIDSIAKDKPLPEKGKLLQAVMKAGPLLQTLLLAGPLPQWRHPPPPLESFEIPPVTIHSPPQPPQHQQQQLLHQESFVNSNCGRFNSRKRVFCEGSDSPSAENKYQRIVLH*