AlfalfaGEDB | Msa0642540

M. sativa cultivar zhongmu-4 / Msa0642540

KEGG: -
GO: -
PFAM: PF07795.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0642540	XP_003606344.1	87.109	256	14	4	1	240	1	253	1.35e-138	399

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0642540	G7JNB1	87.109	256	14	4	1	240	1	253	6.43e-139	399

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0546490	Msa0642540	0.816300	5.938986e-52	-8.615850e-47
Msa0642540	Msa0682020	0.876026	1.935545e-68	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0642540	MtrunA17_Chr4g0028161	87.109	256	14	4	1	240	1	253	1.24e-142	399
Msa0642540	MtrunA17_Chr2g0320271	59.470	264	55	11	1	238	1	238	2.02e-92	271
Msa0642540	MtrunA17_Chr1g0197151	44.311	167	67	3	15	181	29	169	1.08e-34	124
Msa0642540	MtrunA17_Chr4g0030841	36.842	190	68	7	36	186	29	205	1.09e-20	88.2
Msa0642540	MtrunA17_Chr2g0327801	32.222	180	95	5	29	186	30	204	3.23e-18	81.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0642540	AT5G22930.1	50.570	263	80	8	1	239	1	237	3.73e-73	223
Msa0642540	AT3G44940.1	48.315	267	88	9	5	240	3	250	3.07e-56	180
Msa0642540	AT2G28140.1	41.154	260	81	8	1	238	1	210	7.85e-47	155
Msa0642540	AT2G28690.1	33.140	172	112	2	15	185	23	192	5.19e-23	94.0
Msa0642540	AT5G59760.1	31.765	170	107	4	19	186	28	190	2.19e-15	73.2

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Find 62 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACTCCAATGGTGGTGG+AGG	0.517761	4_3:-44294814	None:intergenic
AAATGAGAAAAGAAGAAAAG+GGG	0.530074	4_3:-44293939	None:intergenic
AACAGACAATAGGCAATCTG+TGG	0.649832	4_3:-44293791	None:intergenic
AACGTACCCCTCTTCTATCT+TGG	0.371913	4_3:+44294016	Msa0642540:CDS
AAGGTTGCCTTATAAACCGG+GGG	0.643287	4_3:-44293834	None:intergenic
ACCTTCCTTATACAGTTACT+TGG	0.414957	4_3:+44293852	Msa0642540:five_prime_UTR
ACGTACCCCTCTTCTATCTT+GGG	0.445573	4_3:+44294017	Msa0642540:CDS
AGAAATGAGAAAAGAAGAAA+AGG	0.362600	4_3:-44293941	None:intergenic
AGAGCATAGGGGAGGGAGTT+AGG	0.342485	4_3:+44293762	Msa0642540:five_prime_UTR
AGAGTGTTAGGAGTGTTATG+AGG	0.442989	4_3:-44293910	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCATGTTCTTAGAAAGAAT+GGG	-	chr4_3:44293988-44294007	None:intergenic	30.0%
TCCATGTTCTTAGAAAGAAT+GGG	-	chr4_3:44293988-44294007	None:intergenic	30.0%
ACCTTCCTTATACAGTTACT+TGG	+	chr4_3:44293852-44293871	Msa0642540:five_prime_UTR	35.0%
TCCAAGTAACTGTATAAGGA+AGG	-	chr4_3:44293856-44293875	None:intergenic	35.0%
GTCCATGTTCTTAGAAAGAA+TGG	-	chr4_3:44293989-44294008	None:intergenic	35.0%
AACCTTGATGTACACAACAA+TGG	+	chr4_3:44294333-44294352	Msa0642540:intron	35.0%
CAACAACAACAACAAAAGAG+AGG	+	chr4_3:44294571-44294590	Msa0642540:CDS	35.0%
GGAATTGAATCTTCCAACAA+TGG	+	chr4_3:44294592-44294611	Msa0642540:CDS	35.0%
GATAAACCTTTGCCAGAAAA+AGG	+	chr4_3:44294709-44294728	Msa0642540:CDS	35.0%
ACCTTCCTTATACAGTTACT+TGG	+	chr4_3:44293852-44293871	Msa0642540:five_prime_UTR	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	44293748	44295023	44293748	ID=Msa0642540;Name=Msa0642540
chr4_3	mRNA	44293748	44295023	44293748	ID=Msa0642540-mRNA-1;Parent=Msa0642540;Name=Msa0642540-mRNA-1;_AED=0.20;_eAED=0.20;_QI=255\|0\|0.5\|1\|0\|0.5\|2\|0\|240
chr4_3	exon	44293748	44294059	44293748	ID=Msa0642540-mRNA-1:exon:10145;Parent=Msa0642540-mRNA-1
chr4_3	exon	44294358	44295023	44294358	ID=Msa0642540-mRNA-1:exon:10146;Parent=Msa0642540-mRNA-1
chr4_3	five_prime_UTR	44293748	44294002	44293748	ID=Msa0642540-mRNA-1:five_prime_utr;Parent=Msa0642540-mRNA-1
chr4_3	CDS	44294003	44294059	44294003	ID=Msa0642540-mRNA-1:cds;Parent=Msa0642540-mRNA-1
chr4_3	CDS	44294358	44295023	44294358	ID=Msa0642540-mRNA-1:cds;Parent=Msa0642540-mRNA-1

Gene Sequence

>Msa0642540

ATGGACAATCAACAACGTACCCCTCTTCTATCTTGGGCTTACTACTGCCAAGGAAAGCTTGAACAAACAAGAGTAACAGTACAAGAAGAACTAAGAAAAAGAGATGAACAACTACATAACCTAAAAGAACTTTTCAACAAAGTTGTACGAGAAAGAGATGAAGCACAAGAAAAATGCCAAAGACTTTTCTTAGAAAAGTTACTTTTCCAACAACAAAAACAACAACAAAACCAAGATCCTTTGTCTGGAATCTCAAGCATTGAAGATGAACAACAACAACAACAAAAGAGAGGAATTGAATCTTCCAACAATGGAATATCACTATCAGATTGTGAAGAAAGCATAGTTTCCTCCCCTCAACAAACAAAAACAGAACAACCAATGATGATGATTGATTCAATAGCAAAAGATAAACCTTTGCCAGAAAAAGGTAAACTTTTACAAGCAGTGATGAAAGCTGGTCCTTTGCTTCAGACCCTTTTGCTTGCAGGACCATTACCTCAATGGAGACATCCTCCACCACCATTGGAGTCTTTTGAGATTCCACCTGTGACCATTCATTCACCACCTCAACCACCACAGCATCAGCAGCAACAACTACTTCATCAAGAGTCTTTTGTTAACAGTAACTGTGGAAGATTCAATAGTAGAAAAAGGGTTTTTTGTGAAGGTTCTGATTCTCCTTCTGCTGAGAATAAGTACCAGAGGATTGTACTCCATTGA

Protein sequence

>Msa0642540

MDNQQRTPLLSWAYYCQGKLEQTRVTVQEELRKRDEQLHNLKELFNKVVRERDEAQEKCQRLFLEKLLFQQQKQQQNQDPLSGISSIEDEQQQQQKRGIESSNNGISLSDCEESIVSSPQQTKTEQPMMMIDSIAKDKPLPEKGKLLQAVMKAGPLLQTLLLAGPLPQWRHPPPPLESFEIPPVTIHSPPQPPQHQQQQLLHQESFVNSNCGRFNSRKRVFCEGSDSPSAENKYQRIVLH*