AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0643050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0643050 MtrunA17_Chr4g0029211 97.380 229 6 0 1 229 1 229 8.54e-166 456
Msa0643050 MtrunA17_Chr7g0228161 32.243 214 118 9 31 223 121 328 2.73e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0643050 AT5G60590.2 74.886 219 55 0 1 219 50 268 9.58e-119 340
Msa0643050 AT5G60590.3 73.684 114 30 0 1 114 50 163 9.57e-56 176
Msa0643050 AT5G60590.1 73.451 113 30 0 1 113 50 162 3.08e-55 174

Find 79 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTGTAATAGTGAAATT+AGG 0.277317 4_3:+45493101 Msa0643050:three_prime_UTR
AAGTTATACCATAGGATCTT+TGG 0.291745 4_3:+45492999 Msa0643050:three_prime_UTR
AGGCTCATTGCCCGTGGTTC+AGG 0.295347 4_3:+45491861 Msa0643050:CDS
CATTTGCAGTGCCACGTTCT+AGG 0.302065 4_3:+45492893 Msa0643050:three_prime_UTR
AGTTATACCATAGGATCTTT+GGG 0.323252 4_3:+45493000 Msa0643050:three_prime_UTR
GTGTAGAGAGGTTACCTTCC+AGG 0.342790 4_3:-45492065 None:intergenic
GGTATAACTTATACACTTCA+TGG 0.355899 4_3:-45492986 None:intergenic
CTGCTTGATTCCCTCCTACC+AGG 0.360293 4_3:+45491522 Msa0643050:CDS
GTTCCCACTGATACACTATA+TGG 0.365097 4_3:+45489400 Msa0643050:CDS
GGAAGAGACTGTTGCCATCT+TGG 0.380173 4_3:+45492805 Msa0643050:CDS
TACGGTTGATAAAATTCATT+TGG 0.380601 4_3:+45493200 Msa0643050:three_prime_UTR
GCAGCATAAGCATCTGTTGC+AGG 0.384758 4_3:-45489337 None:intergenic
CCGTGGTTCAGGAACTGCTT+TGG 0.388081 4_3:+45491872 Msa0643050:CDS
CTAAGTATCTTGTATTTGTA+TGG 0.388441 4_3:-45492041 None:intergenic
TTATCACTTAGTAACATGTT+AGG 0.395123 4_3:-45493134 None:intergenic
CTAGAACGTGGCACTGCAAA+TGG 0.395726 4_3:-45492892 None:intergenic
GCTCATTGGAAGCAATTAAC+AGG 0.396765 4_3:+45491394 Msa0643050:intron
CACTTAGATTTGCACTGGTA+AGG 0.398209 4_3:-45491896 None:intergenic
CACTGTGCTTCTGTTTATGA+TGG 0.400620 4_3:+45491966 Msa0643050:CDS
TCTTTGATGCAAACACTACT+TGG 0.413667 4_3:-45491924 None:intergenic
TGCCAGATTGCAATTTCATT+AGG 0.414657 4_3:+45491841 Msa0643050:CDS
TTGGGTCTTGATGTTAAAAC+TGG 0.431335 4_3:+45489304 Msa0643050:CDS
ATGGATTTGCTTGTGATGCT+TGG 0.438588 4_3:+45489419 Msa0643050:CDS
AAAAGCACATTATGAATTAG+TGG 0.440347 4_3:+45493228 Msa0643050:three_prime_UTR
TTGCTTCCATCTGGTCGTGC+AGG 0.447509 4_3:+45491993 Msa0643050:CDS
ACTCCTATACTATCGAATCC+TGG 0.447790 4_3:-45491813 None:intergenic
GTTTCAAACATTGTCAAATG+TGG 0.455160 4_3:-45493034 None:intergenic
GATGGTGGTTTGCTTCCATC+TGG 0.455529 4_3:+45491984 Msa0643050:CDS
GCAGTTGAAGCTTTGAAAGC+TGG 0.459110 4_3:+45489364 Msa0643050:CDS
GTTGATAAAATTCATTTGGT+TGG 0.462398 4_3:+45493204 Msa0643050:three_prime_UTR
TGAAACAGACACATGTGGTC+TGG 0.470574 4_3:+45493051 Msa0643050:three_prime_UTR
CTCACTGATCATTTACCTCA+TGG 0.480763 4_3:+45491498 Msa0643050:CDS
TGTGCTTCTGTTTATGATGG+TGG 0.491904 4_3:+45491969 Msa0643050:CDS
TGCTTGATTCCCTCCTACCA+GGG 0.497345 4_3:+45491523 Msa0643050:CDS
TTTCAAACATTGTCAAATGT+GGG 0.497864 4_3:-45493033 None:intergenic
TTCATGGAAAGAAATTAGCT+TGG 0.498501 4_3:-45492970 None:intergenic
ACTTAGATTTGCACTGGTAA+GGG 0.501601 4_3:-45491895 None:intergenic
CTGTGACAGGCCCTGGTAGG+AGG 0.503303 4_3:-45491533 None:intergenic
CTCCTATACTATCGAATCCT+GGG 0.503774 4_3:-45491812 None:intergenic
GGAACATGTTGGCAGCTCAT+TGG 0.505165 4_3:+45491380 Msa0643050:intron
AGCCTAATGAAATTGCAATC+TGG 0.509551 4_3:-45491843 None:intergenic
AGTACAACTGTGACAGGCCC+TGG 0.510739 4_3:-45491540 None:intergenic
TTGTCCACTTAGATTTGCAC+TGG 0.523144 4_3:-45491901 None:intergenic
AAAAGCATGTTTCTCCAAGA+TGG 0.528414 4_3:-45492819 None:intergenic
CAAAGCAGTTCCTGAACCAC+GGG 0.536721 4_3:-45491871 None:intergenic
TGAAGCTTTGAAAGCTGGAA+AGG 0.541911 4_3:+45489369 Msa0643050:CDS
GAAACAGACACATGTGGTCT+GGG 0.558051 4_3:+45493052 Msa0643050:three_prime_UTR
TGTGACAGGCCCTGGTAGGA+GGG 0.560085 4_3:-45491532 None:intergenic
CGTCTTAGTACAACTGTGAC+AGG 0.567499 4_3:-45491546 None:intergenic
TTCATTAGGCTCATTGCCCG+TGG 0.568961 4_3:+45491855 Msa0643050:CDS
TCGTGCAGGTTCCACTGTTG+TGG 0.581261 4_3:+45492007 Msa0643050:CDS
CTTACCAGTGCAAATCTAAG+TGG 0.582494 4_3:+45491897 Msa0643050:CDS
GTGGAACCTGCACGACCAGA+TGG 0.582499 4_3:-45491999 None:intergenic
AGCCCGCTAGCTATTTGTGT+TGG 0.584508 4_3:+45491447 Msa0643050:CDS
CCAAAGCAGTTCCTGAACCA+CGG 0.585390 4_3:-45491872 None:intergenic
CACCAACACAAATAGCTAGC+GGG 0.589617 4_3:-45491449 None:intergenic
AAGTGTATAAGTTATACCAT+AGG 0.599478 4_3:+45492991 Msa0643050:three_prime_UTR
AACAGATGCTTATGCTGCAG+CGG 0.602354 4_3:+45489342 Msa0643050:CDS
AACCCAGGATTCGATAGTAT+AGG 0.603821 4_3:+45491810 Msa0643050:CDS
GTTATACCATAGGATCTTTG+GGG 0.611090 4_3:+45493001 Msa0643050:three_prime_UTR
CAACTGTGACAGGCCCTGGT+AGG 0.618955 4_3:-45491536 None:intergenic
TCTTGATGTTAAAACTGGTG+TGG 0.621075 4_3:+45489309 Msa0643050:CDS
GAGTATGGAAAGATGTGACT+TGG 0.622957 4_3:+45489285 Msa0643050:CDS
ACAAGATACTTAGACCTGGA+AGG 0.624805 4_3:+45492051 Msa0643050:CDS
AATCCATATAGTGTATCAGT+GGG 0.633215 4_3:-45489403 None:intergenic
AAATCCATATAGTGTATCAG+TGG 0.635491 4_3:-45489404 None:intergenic
ATGAGGTAAATGATCAGTGA+GGG 0.640177 4_3:-45491496 None:intergenic
CTTGAACAATCTCTGAACCC+AGG 0.645214 4_3:+45491795 Msa0643050:CDS
GCTTTCAATTTACAGCGCGA+AGG 0.658974 4_3:+45492784 Msa0643050:intron
GTCACAGTTGTACTAAGACG+AGG 0.662965 4_3:+45491549 Msa0643050:CDS
AAATACAAGATACTTAGACC+TGG 0.674059 4_3:+45492047 Msa0643050:CDS
AAATGATAGAACTTGTGCTA+CGG 0.683093 4_3:+45493182 Msa0643050:three_prime_UTR
AGGGAATCAAGCAGACCATG+AGG 0.692991 4_3:-45491513 None:intergenic
TCACCAACACAAATAGCTAG+CGG 0.694815 4_3:-45491450 None:intergenic
TTATACCATAGGATCTTTGG+GGG 0.695019 4_3:+45493002 Msa0643050:three_prime_UTR
AGTATGGAAAGATGTGACTT+GGG 0.698493 4_3:+45489286 Msa0643050:CDS
ATGTTTGAAACAGACACATG+TGG 0.702632 4_3:+45493046 Msa0643050:three_prime_UTR
CATGAGGTAAATGATCAGTG+AGG 0.709246 4_3:-45491497 None:intergenic
TGTACTGAGATCCACAACAG+TGG 0.735830 4_3:-45492018 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAATGCATATATAAT+AGG - chr4_3:45489535-45489554 None:intergenic 10.0%
!!! TTATTATATATGGTTATTTT+AGG - chr4_3:45489606-45489625 None:intergenic 10.0%
!! GTTCATTTAGTTATTATATA+TGG - chr4_3:45489616-45489635 None:intergenic 15.0%
!! ATTAAAATCAATTAACTGTT+CGG - chr4_3:45490281-45490300 None:intergenic 15.0%
!!! AGTTAATTGATTTTAATTGA+TGG + chr4_3:45490284-45490303 Msa0643050:intron 15.0%
!!! TTCTTTATCAGATTTTTTTT+AGG + chr4_3:45490481-45490500 Msa0643050:intron 15.0%
!!! TCTTTATCAGATTTTTTTTA+GGG + chr4_3:45490482-45490501 Msa0643050:intron 15.0%
!!! ATTGTTAATGTTTGAAATAA+TGG + chr4_3:45491003-45491022 Msa0643050:intron 15.0%
!!! AAATTATTTTGTAAATCACT+TGG - chr4_3:45491216-45491235 None:intergenic 15.0%
!! AATCTTCATATATCTAATAT+AGG + chr4_3:45492166-45492185 Msa0643050:intron 15.0%
!! AAAATTATTTCAAACTTCAA+AGG - chr4_3:45492331-45492350 None:intergenic 15.0%
!! AAACAATTTCAATGCTATAT+AGG - chr4_3:45489492-45489511 None:intergenic 20.0%
!! GAAATGATAAAATTAAACAC+TGG + chr4_3:45489686-45489705 Msa0643050:intron 20.0%
!!! TTTTCAATAGAATGCAAAAT+GGG - chr4_3:45489776-45489795 None:intergenic 20.0%
!!! TTGTTGGTTTTTGAAATAAT+TGG + chr4_3:45490160-45490179 Msa0643050:intron 20.0%
!!! TGTTGGTTTTTGAAATAATT+GGG + chr4_3:45490161-45490180 Msa0643050:intron 20.0%
!!! TTTTTTTTTACAAACAACGA+TGG - chr4_3:45490584-45490603 None:intergenic 20.0%
!!! ATCATATTCTAAAAAATCAC+TGG - chr4_3:45490678-45490697 None:intergenic 20.0%
!! GATTGTTTATTAAACCATTT+AGG + chr4_3:45491151-45491170 Msa0643050:intron 20.0%
!!! AAATGAGATTTTAAAAGTAC+TGG - chr4_3:45491281-45491300 None:intergenic 20.0%
!! TCATAATAGGTCTAAATATA+TGG - chr4_3:45491318-45491337 None:intergenic 20.0%
!!! TGTAATGGAAAATGAATTTA+GGG + chr4_3:45491604-45491623 Msa0643050:intron 20.0%
!!! TCAAAGATTTTGAAAAACTA+TGG + chr4_3:45491940-45491959 Msa0643050:CDS 20.0%
!!! CAAAGATTTTGAAAAACTAT+GGG + chr4_3:45491941-45491960 Msa0643050:CDS 20.0%
!! TTCATATATCTAATATAGGA+TGG + chr4_3:45492170-45492189 Msa0643050:intron 20.0%
!! CATAAAATAACTAACAAAAG+AGG - chr4_3:45492445-45492464 None:intergenic 20.0%
!!! CTCTTTTGTTAGTTATTTTA+TGG + chr4_3:45492443-45492462 Msa0643050:intron 20.0%
!! TATTCTGTAATAGTGAAATT+AGG + chr4_3:45493101-45493120 Msa0643050:three_prime_UTR 20.0%
!! CTTTTCAATAGAATGCAAAA+TGG - chr4_3:45489777-45489796 None:intergenic 25.0%
! CAGTTGAAAGGAAAATAAAT+TGG + chr4_3:45489868-45489887 Msa0643050:intron 25.0%
! GAAAGGAAAATAAATTGGTA+TGG + chr4_3:45489873-45489892 Msa0643050:intron 25.0%
! ATGCAAAGTTACATTATTGT+TGG + chr4_3:45490144-45490163 Msa0643050:intron 25.0%
!! GCTGTTAAAAGATGATTTTA+TGG + chr4_3:45490869-45490888 Msa0643050:intron 25.0%
! ATTAAGAGACAGTTATATAC+CGG + chr4_3:45491027-45491046 Msa0643050:intron 25.0%
! GATAAGTCTTGAAAAATGTA+GGG - chr4_3:45491123-45491142 None:intergenic 25.0%
! AGATAAGTCTTGAAAAATGT+AGG - chr4_3:45491124-45491143 None:intergenic 25.0%
!!! ATTAAACCATTTAGGTTGTT+AGG + chr4_3:45491159-45491178 Msa0643050:intron 25.0%
! GAAAGCAATTGAATCATAAT+AGG - chr4_3:45491331-45491350 None:intergenic 25.0%
!! GTGTAATGGAAAATGAATTT+AGG + chr4_3:45491603-45491622 Msa0643050:intron 25.0%
! GATCTTTACAATGAAATCAA+AGG - chr4_3:45491725-45491744 None:intergenic 25.0%
! CATACAATCATATATTACAC+GGG - chr4_3:45491747-45491766 None:intergenic 25.0%
! GCATACAATCATATATTACA+CGG - chr4_3:45491748-45491767 None:intergenic 25.0%
! CTAAGTATCTTGTATTTGTA+TGG - chr4_3:45492044-45492063 None:intergenic 25.0%
! ATATATCTAATATAGGATGG+TGG + chr4_3:45492173-45492192 Msa0643050:intron 25.0%
!! TATCTAAAACTGAATGATAC+TGG + chr4_3:45492235-45492254 Msa0643050:intron 25.0%
!! TATAAAGCAAACAACCATTT+TGG + chr4_3:45492350-45492369 Msa0643050:intron 25.0%
!! TTGCAATTTCAATACGTTTT+GGG - chr4_3:45492408-45492427 None:intergenic 25.0%
!! TTTGCAATTTCAATACGTTT+TGG - chr4_3:45492409-45492428 None:intergenic 25.0%
!! ATCTCTTCTATTAGTCATTT+TGG - chr4_3:45492529-45492548 None:intergenic 25.0%
! ATAAAAGTAAGCAATCACTT+TGG - chr4_3:45492620-45492639 None:intergenic 25.0%
!!! AAAGTGATTGCTTACTTTTA+TGG + chr4_3:45492619-45492638 Msa0643050:intron 25.0%
!!! AAGTGATTGCTTACTTTTAT+GGG + chr4_3:45492620-45492639 Msa0643050:intron 25.0%
! AACCTAAATTATCTATCGTA+CGG - chr4_3:45492663-45492682 None:intergenic 25.0%
!! GATAATTTAGGTTACTTGTT+TGG + chr4_3:45492670-45492689 Msa0643050:intron 25.0%
!! CAAAAATCAATTCTTTTGCT+TGG + chr4_3:45492929-45492948 Msa0643050:three_prime_UTR 25.0%
! AAGTGTATAAGTTATACCAT+AGG + chr4_3:45492991-45493010 Msa0643050:three_prime_UTR 25.0%
! TTTCAAACATTGTCAAATGT+GGG - chr4_3:45493036-45493055 None:intergenic 25.0%
! TTATCACTTAGTAACATGTT+AGG - chr4_3:45493137-45493156 None:intergenic 25.0%
!!! TACGGTTGATAAAATTCATT+TGG + chr4_3:45493200-45493219 Msa0643050:three_prime_UTR 25.0%
!! GTTGATAAAATTCATTTGGT+TGG + chr4_3:45493204-45493223 Msa0643050:three_prime_UTR 25.0%
!! AAAAGCACATTATGAATTAG+TGG + chr4_3:45493228-45493247 Msa0643050:three_prime_UTR 25.0%
AATCCATATAGTGTATCAGT+GGG - chr4_3:45489406-45489425 None:intergenic 30.0%
AAATCCATATAGTGTATCAG+TGG - chr4_3:45489407-45489426 None:intergenic 30.0%
GGTTTGAGAATGAATTAAAC+AGG + chr4_3:45489707-45489726 Msa0643050:intron 30.0%
AATGAATTAAACAGGTGAAG+TGG + chr4_3:45489715-45489734 Msa0643050:intron 30.0%
!! TGCAAAATGGGTTATTAACT+AGG - chr4_3:45489764-45489783 None:intergenic 30.0%
TTAATGTGGTCAAGCATTAT+AGG - chr4_3:45489834-45489853 None:intergenic 30.0%
!!! TTGCGAGAATAGTTTTGATA+TGG + chr4_3:45489896-45489915 Msa0643050:intron 30.0%
AACAACATTGATATGCCTAT+AGG - chr4_3:45490198-45490217 None:intergenic 30.0%
GCATATCAATGTTGTTATAC+AGG + chr4_3:45490202-45490221 Msa0643050:intron 30.0%
GTTGTTATACAGGTTTAAAC+CGG + chr4_3:45490212-45490231 Msa0643050:intron 30.0%
AGAGAATATGACATTCTTTG+TGG + chr4_3:45490706-45490725 Msa0643050:intron 30.0%
GAGAATATGACATTCTTTGT+GGG + chr4_3:45490707-45490726 Msa0643050:intron 30.0%
TTGTTAACAAAATCAGCAGA+TGG - chr4_3:45490810-45490829 None:intergenic 30.0%
TGTACACTAATGCTATTGAA+AGG - chr4_3:45490834-45490853 None:intergenic 30.0%
! CTAATGTTTTTCCTTGATTG+AGG + chr4_3:45490914-45490933 Msa0643050:intron 30.0%
TGTATGGATATAAAGGTCAA+TGG + chr4_3:45491076-45491095 Msa0643050:intron 30.0%
ATTCATCCTAACAACCTAAA+TGG - chr4_3:45491168-45491187 None:intergenic 30.0%
! TAATAATTGCTTGTTACCTG+TGG + chr4_3:45491359-45491378 Msa0643050:intron 30.0%
TACAAAGGGATTGAAGAAAT+AGG - chr4_3:45491684-45491703 None:intergenic 30.0%
ATTGTATGCTTGAATGTTAC+AGG + chr4_3:45491759-45491778 Msa0643050:intron 30.0%
AAATACAAGATACTTAGACC+TGG + chr4_3:45492047-45492066 Msa0643050:CDS 30.0%
TTGCTGCATTGTTTAAAGAT+TGG + chr4_3:45492094-45492113 Msa0643050:intron 30.0%
GATGTTCTAATATTTCACCA+TGG + chr4_3:45492211-45492230 Msa0643050:intron 30.0%
!!! CATTCAGTTTTAGATATCCA+TGG - chr4_3:45492231-45492250 None:intergenic 30.0%
! ATATGCATTTTGCACATACA+AGG - chr4_3:45492282-45492301 None:intergenic 30.0%
TGCAAAATGCATATGAAACA+CGG + chr4_3:45492288-45492307 Msa0643050:intron 30.0%
AACTAACAAAAGAGGAACTA+GGG - chr4_3:45492437-45492456 None:intergenic 30.0%
TAACTAACAAAAGAGGAACT+AGG - chr4_3:45492438-45492457 None:intergenic 30.0%
! TGTTTGGAAGAAACAGTTTT+TGG + chr4_3:45492686-45492705 Msa0643050:intron 30.0%
TTCATGGAAAGAAATTAGCT+TGG - chr4_3:45492973-45492992 None:intergenic 30.0%
GGTATAACTTATACACTTCA+TGG - chr4_3:45492989-45493008 None:intergenic 30.0%
AAGTTATACCATAGGATCTT+TGG + chr4_3:45492999-45493018 Msa0643050:three_prime_UTR 30.0%
AGTTATACCATAGGATCTTT+GGG + chr4_3:45493000-45493019 Msa0643050:three_prime_UTR 30.0%
GTTTCAAACATTGTCAAATG+TGG - chr4_3:45493037-45493056 None:intergenic 30.0%
AAATGATAGAACTTGTGCTA+CGG + chr4_3:45493182-45493201 Msa0643050:three_prime_UTR 30.0%
AGTATGGAAAGATGTGACTT+GGG + chr4_3:45489286-45489305 Msa0643050:CDS 35.0%
TTGGGTCTTGATGTTAAAAC+TGG + chr4_3:45489304-45489323 Msa0643050:CDS 35.0%
TCTTGATGTTAAAACTGGTG+TGG + chr4_3:45489309-45489328 Msa0643050:CDS 35.0%
!!! GGTTATTTTAGGAGCTTGAT+AGG - chr4_3:45489595-45489614 None:intergenic 35.0%
! CAGTTGCAGATTTTAAGCTA+AGG - chr4_3:45489638-45489657 None:intergenic 35.0%
TTTCAACTGCATGCGAAAAT+GGG - chr4_3:45489857-45489876 None:intergenic 35.0%
TGTCAAAATGCTTGGTTGTT+TGG + chr4_3:45489993-45490012 Msa0643050:intron 35.0%
GTCAAAATGCTTGGTTGTTT+GGG + chr4_3:45489994-45490013 Msa0643050:intron 35.0%
ACAGCAGTAATGTGTGATAT+AGG + chr4_3:45490107-45490126 Msa0643050:intron 35.0%
CAGCAGTAATGTGTGATATA+GGG + chr4_3:45490108-45490127 Msa0643050:intron 35.0%
AAGACATATGCATACACATC+TGG - chr4_3:45490432-45490451 None:intergenic 35.0%
GATTCACTTAACCTCAATCA+AGG - chr4_3:45490928-45490947 None:intergenic 35.0%
TTAAAATGTGCGGCTAAGTT+TGG - chr4_3:45490950-45490969 None:intergenic 35.0%
GCAAAGTTGCTTAAAATGTG+CGG - chr4_3:45490960-45490979 None:intergenic 35.0%
AGCAACTTTGCTCAATAAAC+AGG + chr4_3:45490969-45490988 Msa0643050:intron 35.0%
GCAACTTTGCTCAATAAACA+GGG + chr4_3:45490970-45490989 Msa0643050:intron 35.0%
CAACTTTGCTCAATAAACAG+GGG + chr4_3:45490971-45490990 Msa0643050:intron 35.0%
AACTTTGCTCAATAAACAGG+GGG + chr4_3:45490972-45490991 Msa0643050:intron 35.0%
GGATAAAACAAGCAAAGTTC+CGG - chr4_3:45491049-45491068 None:intergenic 35.0%
!!! ACTTTGCTTGTTTTATCCTC+TGG + chr4_3:45491051-45491070 Msa0643050:intron 35.0%
!!! CTTTGCTTGTTTTATCCTCT+GGG + chr4_3:45491052-45491071 Msa0643050:intron 35.0%
TTTATATCCATACATCCCAG+AGG - chr4_3:45491070-45491089 None:intergenic 35.0%
! TCTGGGATGTATGGATATAA+AGG + chr4_3:45491069-45491088 Msa0643050:intron 35.0%
ATGGATATAAAGGTCAATGG+AGG + chr4_3:45491079-45491098 Msa0643050:intron 35.0%
ATGAGGTAAATGATCAGTGA+GGG - chr4_3:45491499-45491518 None:intergenic 35.0%
TCACACACAAAAACAACAGA+TGG - chr4_3:45491583-45491602 None:intergenic 35.0%
! GTTTTTGTGTGTGAGTGTAA+TGG + chr4_3:45491589-45491608 Msa0643050:intron 35.0%
CAGAAGAAATGCTGTACAAA+GGG - chr4_3:45491698-45491717 None:intergenic 35.0%
ACAGAAGAAATGCTGTACAA+AGG - chr4_3:45491699-45491718 None:intergenic 35.0%
TGCCAGATTGCAATTTCATT+AGG + chr4_3:45491841-45491860 Msa0643050:CDS 35.0%
AGCCTAATGAAATTGCAATC+TGG - chr4_3:45491846-45491865 None:intergenic 35.0%
! ACTTAGATTTGCACTGGTAA+GGG - chr4_3:45491898-45491917 None:intergenic 35.0%
TCTTTGATGCAAACACTACT+TGG - chr4_3:45491927-45491946 None:intergenic 35.0%
CAGCAAAAATAAGTGTAGAG+AGG - chr4_3:45492080-45492099 None:intergenic 35.0%
! ATCTAATATAGGATGGTGGT+AGG + chr4_3:45492177-45492196 Msa0643050:intron 35.0%
ACATACAAGGTAACCTAAGT+GGG - chr4_3:45492269-45492288 None:intergenic 35.0%
CAAACATTCAAGGACCAAAA+TGG - chr4_3:45492367-45492386 None:intergenic 35.0%
! TTTTGGTCCTTGAATGTTTG+AGG + chr4_3:45492367-45492386 Msa0643050:intron 35.0%
ACCAACGAAAAACACATTCA+AGG + chr4_3:45492470-45492489 Msa0643050:intron 35.0%
! TCCTTGAATGTGTTTTTCGT+TGG - chr4_3:45492474-45492493 None:intergenic 35.0%
ACTAATAGAAGAGATACGTG+TGG + chr4_3:45492534-45492553 Msa0643050:intron 35.0%
TTGCAATTTCGATGCATTCA+AGG + chr4_3:45492563-45492582 Msa0643050:intron 35.0%
AGCCGTACGATAGATAATTT+AGG + chr4_3:45492658-45492677 Msa0643050:intron 35.0%
CTGTAAATTGAAAGCGAACA+AGG - chr4_3:45492779-45492798 None:intergenic 35.0%
! AAAAGCATGTTTCTCCAAGA+TGG - chr4_3:45492822-45492841 None:intergenic 35.0%
GTTATACCATAGGATCTTTG+GGG + chr4_3:45493001-45493020 Msa0643050:three_prime_UTR 35.0%
TTATACCATAGGATCTTTGG+GGG + chr4_3:45493002-45493021 Msa0643050:three_prime_UTR 35.0%
! ATGTTTGAAACAGACACATG+TGG + chr4_3:45493046-45493065 Msa0643050:three_prime_UTR 35.0%
GAGTATGGAAAGATGTGACT+TGG + chr4_3:45489285-45489304 Msa0643050:CDS 40.0%
!! TGAAGCTTTGAAAGCTGGAA+AGG + chr4_3:45489369-45489388 Msa0643050:CDS 40.0%
GTTCCCACTGATACACTATA+TGG + chr4_3:45489400-45489419 Msa0643050:CDS 40.0%
! ATGGATTTGCTTGTGATGCT+TGG + chr4_3:45489419-45489438 Msa0643050:CDS 40.0%
GGTCAAGCATTATAGGCATT+TGG - chr4_3:45489827-45489846 None:intergenic 40.0%
!! CATGCGAAAATGGGTTAATG+TGG - chr4_3:45489848-45489867 None:intergenic 40.0%
CTTTCAACTGCATGCGAAAA+TGG - chr4_3:45489858-45489877 None:intergenic 40.0%
! CATTTTCGCATGCAGTTGAA+AGG + chr4_3:45489856-45489875 Msa0643050:intron 40.0%
GCTGAACTGAATCATAGCAT+CGG + chr4_3:45489940-45489959 Msa0643050:intron 40.0%
CACTGCATTGTCAAAATGCT+TGG + chr4_3:45489985-45490004 Msa0643050:intron 40.0%
AAAATGCTTGGTTGTTTGGG+TGG + chr4_3:45489997-45490016 Msa0643050:intron 40.0%
AAATGCTTGGTTGTTTGGGT+GGG + chr4_3:45489998-45490017 Msa0643050:intron 40.0%
CAGTAGCGTGGAAACTTAAA+TGG + chr4_3:45490023-45490042 Msa0643050:intron 40.0%
TGGGTTCAACTTTAGCCTAT+AGG + chr4_3:45490180-45490199 Msa0643050:intron 40.0%
TATACAGGTTTAAACCGGTG+TGG + chr4_3:45490217-45490236 Msa0643050:intron 40.0%
AGACTATAAAGCAACCACAC+CGG - chr4_3:45490234-45490253 None:intergenic 40.0%
! GTGGTTGCTTTATAGTCTGT+TGG + chr4_3:45490236-45490255 Msa0643050:intron 40.0%
TTCACAGTTCGACTGCATTT+CGG + chr4_3:45490365-45490384 Msa0643050:intron 40.0%
ATATAGACGCAGAGGATTCA+TGG - chr4_3:45490557-45490576 None:intergenic 40.0%
ATGGATACATATAGACGCAG+AGG - chr4_3:45490565-45490584 None:intergenic 40.0%
!!! GTTTTATCCTCTGGGATGTA+TGG + chr4_3:45491060-45491079 Msa0643050:intron 40.0%
!! TCACTTGGTTGATAGTTTGC+TGG - chr4_3:45491201-45491220 None:intergenic 40.0%
TTGTTACCTGTGGAACATGT+TGG + chr4_3:45491369-45491388 Msa0643050:intron 40.0%
GCTCATTGGAAGCAATTAAC+AGG + chr4_3:45491394-45491413 Msa0643050:intron 40.0%
TCACCAACACAAATAGCTAG+CGG - chr4_3:45491453-45491472 None:intergenic 40.0%
CATGAGGTAAATGATCAGTG+AGG - chr4_3:45491500-45491519 None:intergenic 40.0%
CTCACTGATCATTTACCTCA+TGG + chr4_3:45491498-45491517 Msa0643050:CDS 40.0%
AACCCAGGATTCGATAGTAT+AGG + chr4_3:45491810-45491829 Msa0643050:CDS 40.0%
CTCCTATACTATCGAATCCT+GGG - chr4_3:45491815-45491834 None:intergenic 40.0%
ACTCCTATACTATCGAATCC+TGG - chr4_3:45491816-45491835 None:intergenic 40.0%
! CACTTAGATTTGCACTGGTA+AGG - chr4_3:45491899-45491918 None:intergenic 40.0%
CTTACCAGTGCAAATCTAAG+TGG + chr4_3:45491897-45491916 Msa0643050:CDS 40.0%
TTGTCCACTTAGATTTGCAC+TGG - chr4_3:45491904-45491923 None:intergenic 40.0%
CACTGTGCTTCTGTTTATGA+TGG + chr4_3:45491966-45491985 Msa0643050:CDS 40.0%
TGTGCTTCTGTTTATGATGG+TGG + chr4_3:45491969-45491988 Msa0643050:CDS 40.0%
ACAAGATACTTAGACCTGGA+AGG + chr4_3:45492051-45492070 Msa0643050:CDS 40.0%
CACATACAAGGTAACCTAAG+TGG - chr4_3:45492270-45492289 None:intergenic 40.0%
TGACAACGCTTCATACATTC+AGG + chr4_3:45492593-45492612 Msa0643050:intron 40.0%
GACAACGCTTCATACATTCA+GGG + chr4_3:45492594-45492613 Msa0643050:intron 40.0%
! GGAGAAACATGCTTTTGTTG+AGG + chr4_3:45492826-45492845 Msa0643050:CDS 40.0%
AGCGAAAAATACCTAGAACG+TGG - chr4_3:45492907-45492926 None:intergenic 40.0%
! TTCTTTTGCTTGGACATGAG+AGG + chr4_3:45492939-45492958 Msa0643050:three_prime_UTR 40.0%
AACAGATGCTTATGCTGCAG+CGG + chr4_3:45489342-45489361 Msa0643050:CDS 45.0%
!! GCAGTTGAAGCTTTGAAAGC+TGG + chr4_3:45489364-45489383 Msa0643050:CDS 45.0%
GTTGCGAGAACTAAGCAAGT+AGG + chr4_3:45489918-45489937 Msa0643050:intron 45.0%
TCACAGTTCGACTGCATTTC+GGG + chr4_3:45490366-45490385 Msa0643050:intron 45.0%
TTTGCTCAATAAACAGGGGG+TGG + chr4_3:45490975-45490994 Msa0643050:intron 45.0%
CACCAACACAAATAGCTAGC+GGG - chr4_3:45491452-45491471 None:intergenic 45.0%
CGTCTTAGTACAACTGTGAC+AGG - chr4_3:45491549-45491568 None:intergenic 45.0%
GTCACAGTTGTACTAAGACG+AGG + chr4_3:45491549-45491568 Msa0643050:CDS 45.0%
CTTGAACAATCTCTGAACCC+AGG + chr4_3:45491795-45491814 Msa0643050:CDS 45.0%
TGTACTGAGATCCACAACAG+TGG - chr4_3:45492021-45492040 None:intergenic 45.0%
ACTGGATTCATCTCCCACTT+AGG + chr4_3:45492253-45492272 Msa0643050:intron 45.0%
GACAACGCCTCAAACATTCA+AGG - chr4_3:45492377-45492396 None:intergenic 45.0%
!! TGTTTGAGGCGTTGTCACTA+TGG + chr4_3:45492381-45492400 Msa0643050:intron 45.0%
GCTTTCAATTTACAGCGCGA+AGG + chr4_3:45492784-45492803 Msa0643050:intron 45.0%
TGAAACAGACACATGTGGTC+TGG + chr4_3:45493051-45493070 Msa0643050:three_prime_UTR 45.0%
GAAACAGACACATGTGGTCT+GGG + chr4_3:45493052-45493071 Msa0643050:three_prime_UTR 45.0%
GCAGCATAAGCATCTGTTGC+AGG - chr4_3:45489340-45489359 None:intergenic 50.0%
CACAGTTCGACTGCATTTCG+GGG + chr4_3:45490367-45490386 Msa0643050:intron 50.0%
GGAACATGTTGGCAGCTCAT+TGG + chr4_3:45491380-45491399 Msa0643050:intron 50.0%
AGCCCGCTAGCTATTTGTGT+TGG + chr4_3:45491447-45491466 Msa0643050:CDS 50.0%
AGGGAATCAAGCAGACCATG+AGG - chr4_3:45491516-45491535 None:intergenic 50.0%
TGCTTGATTCCCTCCTACCA+GGG + chr4_3:45491523-45491542 Msa0643050:CDS 50.0%
TTCATTAGGCTCATTGCCCG+TGG + chr4_3:45491855-45491874 Msa0643050:CDS 50.0%
CAAAGCAGTTCCTGAACCAC+GGG - chr4_3:45491874-45491893 None:intergenic 50.0%
CCAAAGCAGTTCCTGAACCA+CGG - chr4_3:45491875-45491894 None:intergenic 50.0%
!! GATGGTGGTTTGCTTCCATC+TGG + chr4_3:45491984-45492003 Msa0643050:CDS 50.0%
GTGTAGAGAGGTTACCTTCC+AGG - chr4_3:45492068-45492087 None:intergenic 50.0%
GGAAGAGACTGTTGCCATCT+TGG + chr4_3:45492805-45492824 Msa0643050:CDS 50.0%
CTAGAACGTGGCACTGCAAA+TGG - chr4_3:45492895-45492914 None:intergenic 50.0%
CATTTGCAGTGCCACGTTCT+AGG + chr4_3:45492893-45492912 Msa0643050:three_prime_UTR 50.0%
ATGCACCCCCAAAGATCCTA+TGG - chr4_3:45493010-45493029 None:intergenic 50.0%
TTTGGGTGGGAACAGTAGCG+TGG + chr4_3:45490011-45490030 Msa0643050:intron 55.0%
GAGCTGCCAACATGTTCCAC+AGG - chr4_3:45491378-45491397 None:intergenic 55.0%
CTGCTTGATTCCCTCCTACC+AGG + chr4_3:45491522-45491541 Msa0643050:CDS 55.0%
AGTACAACTGTGACAGGCCC+TGG - chr4_3:45491543-45491562 None:intergenic 55.0%
CCGTGGTTCAGGAACTGCTT+TGG + chr4_3:45491872-45491891 Msa0643050:CDS 55.0%
TTGCTTCCATCTGGTCGTGC+AGG + chr4_3:45491993-45492012 Msa0643050:CDS 55.0%
TCGTGCAGGTTCCACTGTTG+TGG + chr4_3:45492007-45492026 Msa0643050:CDS 55.0%
TGTGACAGGCCCTGGTAGGA+GGG - chr4_3:45491535-45491554 None:intergenic 60.0%
CAACTGTGACAGGCCCTGGT+AGG - chr4_3:45491539-45491558 None:intergenic 60.0%
AGGCTCATTGCCCGTGGTTC+AGG + chr4_3:45491861-45491880 Msa0643050:CDS 60.0%
GTGGAACCTGCACGACCAGA+TGG - chr4_3:45492002-45492021 None:intergenic 60.0%
CTGTGACAGGCCCTGGTAGG+AGG - chr4_3:45491536-45491555 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 45489273 45493268 45489273 ID=Msa0643050;Name=Msa0643050
chr4_3 mRNA 45489273 45493268 45489273 ID=Msa0643050-mRNA-1;Parent=Msa0643050;Name=Msa0643050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=4|1|1|1|1|1|4|403|232
chr4_3 exon 45489273 45489440 45489273 ID=Msa0643050-mRNA-1:exon:10509;Parent=Msa0643050-mRNA-1
chr4_3 exon 45491395 45491570 45491395 ID=Msa0643050-mRNA-1:exon:10510;Parent=Msa0643050-mRNA-1
chr4_3 exon 45491781 45492072 45491781 ID=Msa0643050-mRNA-1:exon:10511;Parent=Msa0643050-mRNA-1
chr4_3 exon 45492799 45493268 45492799 ID=Msa0643050-mRNA-1:exon:10512;Parent=Msa0643050-mRNA-1
chr4_3 five_prime_UTR 45489273 45489276 45489273 ID=Msa0643050-mRNA-1:five_prime_utr;Parent=Msa0643050-mRNA-1
chr4_3 CDS 45489277 45489440 45489277 ID=Msa0643050-mRNA-1:cds;Parent=Msa0643050-mRNA-1
chr4_3 CDS 45491395 45491570 45491395 ID=Msa0643050-mRNA-1:cds;Parent=Msa0643050-mRNA-1
chr4_3 CDS 45491781 45492072 45491781 ID=Msa0643050-mRNA-1:cds;Parent=Msa0643050-mRNA-1
chr4_3 CDS 45492799 45492865 45492799 ID=Msa0643050-mRNA-1:cds;Parent=Msa0643050-mRNA-1
chr4_3 three_prime_UTR 45492866 45493268 45492866 ID=Msa0643050-mRNA-1:three_prime_utr;Parent=Msa0643050-mRNA-1
Gene Sequence

>Msa0643050

ATGGCTTGGAGTATGGAAAGATGTGACTTGGGTCTTGATGTTAAAACTGGTGTGGTTCATCCTGCAACAGATGCTTATGCTGCAGCGGCAGTTGAAGCTTTGAAAGCTGGAAAGGTCATTGCAGTTCCCACTGATACACTATATGGATTTGCTTGTGATGCTTGCTCATTGGAAGCAATTAACAGGATTTATGAGATTAAAGATCGTAAACATACAAGCCCGCTAGCTATTTGTGTTGGTGATGTATCAGACATAAATCGCTTTGCCCTCACTGATCATTTACCTCATGGTCTGCTTGATTCCCTCCTACCAGGGCCTGTCACAGTTGTACTAAGACGAGGTGAGTCAAGTGCGCTTGAACAATCTCTGAACCCAGGATTCGATAGTATAGGAGTTAGAGTGCCAGATTGCAATTTCATTAGGCTCATTGCCCGTGGTTCAGGAACTGCTTTGGCCCTTACCAGTGCAAATCTAAGTGGACAACCAAGTAGTGTTTGCATCAAAGATTTTGAAAAACTATGGGAGCACTGTGCTTCTGTTTATGATGGTGGTTTGCTTCCATCTGGTCGTGCAGGTTCCACTGTTGTGGATCTCAGTACACCATACAAATACAAGATACTTAGACCTGGAAGCGCGAAGGAAGAGACTGTTGCCATCTTGGAGAAACATGCTTTTGTTGAGGAAGTGCGACATACTTAA
Protein sequence

>Msa0643050

MAWSMERCDLGLDVKTGVVHPATDAYAAAAVEALKAGKVIAVPTDTLYGFACDACSLEAINRIYEIKDRKHTSPLAICVGDVSDINRFALTDHLPHGLLDSLLPGPVTVVLRRGESSALEQSLNPGFDSIGVRVPDCNFIRLIARGSGTALALTSANLSGQPSSVCIKDFEKLWEHCASVYDGGLLPSGRAGSTVVDLSTPYKYKILRPGSAKEETVAILEKHAFVEEVRHT*