AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0651100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651100 MtrunA17_Chr4g0043481 97.041 169 4 1 1 168 1 169 4.05e-118 330
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651100 AT2G04340.1 56.522 161 68 2 6 166 13 171 3.30e-64 195

Find 67 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAAGACCAACGTGTTT+TGG 0.194073 4_3:+58404179 Msa0651100:five_prime_UTR
TAACTAGTGGTTTATACTTA+TGG 0.211874 4_3:+58405659 Msa0651100:three_prime_UTR
TCCAGTTCTGATTCTGGAAT+TGG 0.212325 4_3:-58404723 None:intergenic
TTGGATTCATACGGAGTTCT+AGG 0.245059 4_3:-58404689 None:intergenic
GGAAAATTGCCTGAACTCTT+TGG 0.253591 4_3:+58405559 Msa0651100:three_prime_UTR
AGCCGACAATTAAGTTTATT+AGG 0.286121 4_3:+58405464 Msa0651100:three_prime_UTR
TCGAGGTAGGATAAGGATTC+AGG 0.290716 4_3:-58404381 None:intergenic
AAAAGAAATCAAACATAAAA+AGG 0.290843 4_3:-58405697 None:intergenic
CAAAACACGTTGGTCTTTAG+AGG 0.314898 4_3:-58404178 None:intergenic
ATGCACTCTTAAGAAAATAA+AGG 0.322223 4_3:+58405635 Msa0651100:three_prime_UTR
AACTAGTGGTTTATACTTAT+GGG 0.322469 4_3:+58405660 Msa0651100:three_prime_UTR
ATCTGAAGCATTGAAGAAAA+AGG 0.323646 4_3:+58404290 Msa0651100:CDS
CTCACAGTACCTTGACCTTC+AGG 0.325447 4_3:-58404623 None:intergenic
TTTGTCTTATTACGTTCATT+AGG 0.328633 4_3:-58404233 None:intergenic
CCAGTTCTGATTCTGGAATT+GGG 0.353599 4_3:-58404722 None:intergenic
ATTTACCATCATGAGAAGTT+TGG 0.355493 4_3:-58404454 None:intergenic
TCCTACGCTCCAACAACTCT+TGG 0.371569 4_3:-58404589 None:intergenic
ACATGCAACCAGATGCAAAA+TGG 0.373896 4_3:+58405585 Msa0651100:three_prime_UTR
GCATGTTTGCCAAAGAGTTC+AGG 0.380995 4_3:-58405568 None:intergenic
CGTAACGATGAACTGGTCTT+TGG 0.392657 4_3:-58404355 None:intergenic
CACCTAATAAACTTAATTGT+CGG 0.406028 4_3:-58405466 None:intergenic
TTTCAAGTATCCATGCTTCT+CGG 0.412618 4_3:-58405746 None:intergenic
CTCCAACAACTCTTGGGATC+TGG 0.424915 4_3:-58404582 None:intergenic
ATCCAGATCCCAAGAGTTGT+TGG 0.426994 4_3:+58404580 Msa0651100:CDS
AAAACAACAATCAATCAAAC+CGG 0.431424 4_3:+58404207 Msa0651100:five_prime_UTR
GGAATTGGGACGAGAAAACT+TGG 0.437414 4_3:-58404708 None:intergenic
GCCAACAGCAATGCCGATCA+TGG 0.451912 4_3:-58404418 None:intergenic
CCCAATTCCAGAATCAGAAC+TGG 0.465325 4_3:+58404722 Msa0651100:CDS
GTTGAAAATGAAGGTTATTG+AGG 0.470958 4_3:-58405534 None:intergenic
TGCGGTGTCGAGGTAGGATA+AGG 0.473913 4_3:-58404388 None:intergenic
TGGAGAAGAAGAGGAAGAAG+AGG 0.480327 4_3:+58404322 Msa0651100:CDS
TTTCGCCAAACTTCTCATGA+TGG 0.487048 4_3:+58404449 Msa0651100:CDS
CAGGGACCGTAACGATGAAC+TGG 0.487342 4_3:-58404362 None:intergenic
GAACCTTGTCCCACTCTTCA+CGG 0.488340 4_3:-58404655 None:intergenic
ATTAAGTTTATTAGGTGTTG+TGG 0.490415 4_3:+58405472 Msa0651100:three_prime_UTR
CCTACGCTCCAACAACTCTT+GGG 0.506302 4_3:-58404588 None:intergenic
CTTAATTGTCGGCTAATACA+AGG 0.515960 4_3:-58405455 None:intergenic
TACGACATGTTACATTACTT+AGG 0.517159 4_3:-58405425 None:intergenic
TTTACCATAGTCTTCAGCTC+TGG 0.519072 4_3:-58405396 None:intergenic
CATTGGAATTGGAGAAGAAG+AGG 0.524044 4_3:+58404313 Msa0651100:CDS
CCCATGATCGGCATTGCTGT+TGG 0.525769 4_3:+58404417 Msa0651100:CDS
CGAGGTAGGATAAGGATTCA+GGG 0.534228 4_3:-58404380 None:intergenic
GTTGCCAGAGCTGAAGACTA+TGG 0.536885 4_3:+58405392 Msa0651100:CDS
ACCGCATCTCTGCCCATGAT+CGG 0.540473 4_3:+58404405 Msa0651100:CDS
TACAGGAGGACGTAAAGGAT+TGG 0.546228 4_3:+58405345 Msa0651100:intron
GGGACAAGGTTCGAGAACTC+AGG 0.548800 4_3:+58404666 Msa0651100:CDS
CAAAGACCAGTTCATCGTTA+CGG 0.551542 4_3:+58404356 Msa0651100:CDS
GGAGAAGAAGAGGAAGAAGA+GGG 0.551810 4_3:+58404323 Msa0651100:CDS
CAGAGATGCGGTGTCGAGGT+AGG 0.553355 4_3:-58404394 None:intergenic
ATGTCTACAGGAGGACGTAA+AGG 0.556570 4_3:+58405340 Msa0651100:intron
TTCAATTTGACCGAGAAGCA+TGG 0.559789 4_3:+58405736 Msa0651100:three_prime_UTR
CGAGAAAACTTGGATTCATA+CGG 0.560787 4_3:-58404698 None:intergenic
GAATGCTCAGCCGTGAAGAG+TGG 0.566528 4_3:+58404645 Msa0651100:CDS
AATGCTCAGCCGTGAAGAGT+GGG 0.573652 4_3:+58404646 Msa0651100:CDS
TGGTGATTTATGTTATGACA+TGG 0.576344 4_3:+58405492 Msa0651100:three_prime_UTR
CCAACAGCAATGCCGATCAT+GGG 0.576515 4_3:-58404417 None:intergenic
CCCAAGAGTTGTTGGAGCGT+AGG 0.581873 4_3:+58404588 Msa0651100:CDS
AGAAAATAAAGGATAACTAG+TGG 0.593663 4_3:+58405646 Msa0651100:three_prime_UTR
TATTACGTTCATTAGGAACC+CGG 0.599628 4_3:-58404226 None:intergenic
GCCGATCATGGGCAGAGATG+CGG 0.599977 4_3:-58404406 None:intergenic
AGGATAAAGAATGCTCCTGA+AGG 0.601200 4_3:+58404608 Msa0651100:CDS
AAGAATGCTCCTGAAGGTCA+AGG 0.605809 4_3:+58404614 Msa0651100:CDS
TGGGCAGAGATGCGGTGTCG+AGG 0.615374 4_3:-58404398 None:intergenic
CAGCCGTGAAGAGTGGGACA+AGG 0.626546 4_3:+58404652 Msa0651100:CDS
GCTGAAGACTATGGTAAACA+TGG 0.649911 4_3:+58405401 Msa0651100:CDS
AAACAACAATCAATCAAACC+GGG 0.669116 4_3:+58404208 Msa0651100:five_prime_UTR
AGATTGTTACACACACTGCG+TGG 0.702041 4_3:+58404255 Msa0651100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATTTTTAAAATTATTAC+CGG + chr4_3:58404922-58404941 Msa0651100:intron 10.0%
!!! TCTATTTTTAAAATTATTAC+CGG + chr4_3:58404922-58404941 Msa0651100:intron 10.0%
!!! AATTTTAAAAATAGAAGTGA+TGG - chr4_3:58404917-58404936 None:intergenic 15.0%
!! AAAAGAAATCAAACATAAAA+AGG - chr4_3:58405700-58405719 None:intergenic 15.0%
!!! AATTTTAAAAATAGAAGTGA+TGG - chr4_3:58404917-58404936 None:intergenic 15.0%
!! AAAAGAAATCAAACATAAAA+AGG - chr4_3:58405700-58405719 None:intergenic 15.0%
! AAAACAACAATCAATCAAAC+CGG + chr4_3:58404207-58404226 Msa0651100:five_prime_UTR 25.0%
!! TTTGTCTTATTACGTTCATT+AGG - chr4_3:58404236-58404255 None:intergenic 25.0%
! AGTGAAAATTAGTCACTAAT+GGG + chr4_3:58404532-58404551 Msa0651100:intron 25.0%
! TTTCTTTGTTCATTGTATAC+AGG + chr4_3:58405083-58405102 Msa0651100:intron 25.0%
! TTCTTTGTTCATTGTATACA+GGG + chr4_3:58405084-58405103 Msa0651100:intron 25.0%
! ATGTAGTAATTCTACATGAT+TGG + chr4_3:58405142-58405161 Msa0651100:intron 25.0%
!!! GCTTTGGAATATATAATACA+AGG + chr4_3:58405280-58405299 Msa0651100:intron 25.0%
!!! TTTTGTTATATGATGTCTAC+AGG + chr4_3:58405328-58405347 Msa0651100:intron 25.0%
! CACCTAATAAACTTAATTGT+CGG - chr4_3:58405469-58405488 None:intergenic 25.0%
!! ATTAAGTTTATTAGGTGTTG+TGG + chr4_3:58405472-58405491 Msa0651100:three_prime_UTR 25.0%
! ATGCACTCTTAAGAAAATAA+AGG + chr4_3:58405635-58405654 Msa0651100:three_prime_UTR 25.0%
! AGAAAATAAAGGATAACTAG+TGG + chr4_3:58405646-58405665 Msa0651100:three_prime_UTR 25.0%
! TAACTAGTGGTTTATACTTA+TGG + chr4_3:58405659-58405678 Msa0651100:three_prime_UTR 25.0%
! AACTAGTGGTTTATACTTAT+GGG + chr4_3:58405660-58405679 Msa0651100:three_prime_UTR 25.0%
! AAAACAACAATCAATCAAAC+CGG + chr4_3:58404207-58404226 Msa0651100:five_prime_UTR 25.0%
!! TTTGTCTTATTACGTTCATT+AGG - chr4_3:58404236-58404255 None:intergenic 25.0%
! AGTGAAAATTAGTCACTAAT+GGG + chr4_3:58404532-58404551 Msa0651100:intron 25.0%
! TTTCTTTGTTCATTGTATAC+AGG + chr4_3:58405083-58405102 Msa0651100:intron 25.0%
! TTCTTTGTTCATTGTATACA+GGG + chr4_3:58405084-58405103 Msa0651100:intron 25.0%
! ATGTAGTAATTCTACATGAT+TGG + chr4_3:58405142-58405161 Msa0651100:intron 25.0%
!!! GCTTTGGAATATATAATACA+AGG + chr4_3:58405280-58405299 Msa0651100:intron 25.0%
!!! TTTTGTTATATGATGTCTAC+AGG + chr4_3:58405328-58405347 Msa0651100:intron 25.0%
! CACCTAATAAACTTAATTGT+CGG - chr4_3:58405469-58405488 None:intergenic 25.0%
!! ATTAAGTTTATTAGGTGTTG+TGG + chr4_3:58405472-58405491 Msa0651100:three_prime_UTR 25.0%
! ATGCACTCTTAAGAAAATAA+AGG + chr4_3:58405635-58405654 Msa0651100:three_prime_UTR 25.0%
! AGAAAATAAAGGATAACTAG+TGG + chr4_3:58405646-58405665 Msa0651100:three_prime_UTR 25.0%
! TAACTAGTGGTTTATACTTA+TGG + chr4_3:58405659-58405678 Msa0651100:three_prime_UTR 25.0%
! AACTAGTGGTTTATACTTAT+GGG + chr4_3:58405660-58405679 Msa0651100:three_prime_UTR 25.0%
AAACAACAATCAATCAAACC+GGG + chr4_3:58404208-58404227 Msa0651100:five_prime_UTR 30.0%
! TTTTCTTCAATGCTTCAGAT+GGG - chr4_3:58404290-58404309 None:intergenic 30.0%
! TTTTTCTTCAATGCTTCAGA+TGG - chr4_3:58404291-58404310 None:intergenic 30.0%
!! ATCTGAAGCATTGAAGAAAA+AGG + chr4_3:58404290-58404309 Msa0651100:CDS 30.0%
ATTTACCATCATGAGAAGTT+TGG - chr4_3:58404457-58404476 None:intergenic 30.0%
CAGTGAAAATTAGTCACTAA+TGG + chr4_3:58404531-58404550 Msa0651100:intron 30.0%
GACACTTTGAATTGAAAAAG+TGG + chr4_3:58404844-58404863 Msa0651100:intron 30.0%
ACTCTTGGTTAAAACATGTT+AGG - chr4_3:58405044-58405063 None:intergenic 30.0%
AACATATAGTGAATGACTCT+TGG - chr4_3:58405059-58405078 None:intergenic 30.0%
! AGATCATAAGAGTTTTGCTT+TGG + chr4_3:58405264-58405283 Msa0651100:intron 30.0%
! AAGGAAATCATTTAGTTTCG+TGG + chr4_3:58405299-58405318 Msa0651100:intron 30.0%
! TTGGACAATTGATGTTTTCA+TGG + chr4_3:58405364-58405383 Msa0651100:CDS 30.0%
! ACAATTGATGTTTTCATGGA+TGG + chr4_3:58405368-58405387 Msa0651100:CDS 30.0%
TACGACATGTTACATTACTT+AGG - chr4_3:58405428-58405447 None:intergenic 30.0%
AGCCGACAATTAAGTTTATT+AGG + chr4_3:58405464-58405483 Msa0651100:three_prime_UTR 30.0%
TGGTGATTTATGTTATGACA+TGG + chr4_3:58405492-58405511 Msa0651100:three_prime_UTR 30.0%
GTTGAAAATGAAGGTTATTG+AGG - chr4_3:58405537-58405556 None:intergenic 30.0%
! AATAACCTTCATTTTCAACC+TGG + chr4_3:58405538-58405557 Msa0651100:three_prime_UTR 30.0%
!! ATTTTCCAGGTTGAAAATGA+AGG - chr4_3:58405546-58405565 None:intergenic 30.0%
AAACAACAATCAATCAAACC+GGG + chr4_3:58404208-58404227 Msa0651100:five_prime_UTR 30.0%
! TTTTCTTCAATGCTTCAGAT+GGG - chr4_3:58404290-58404309 None:intergenic 30.0%
! TTTTTCTTCAATGCTTCAGA+TGG - chr4_3:58404291-58404310 None:intergenic 30.0%
!! ATCTGAAGCATTGAAGAAAA+AGG + chr4_3:58404290-58404309 Msa0651100:CDS 30.0%
ATTTACCATCATGAGAAGTT+TGG - chr4_3:58404457-58404476 None:intergenic 30.0%
CAGTGAAAATTAGTCACTAA+TGG + chr4_3:58404531-58404550 Msa0651100:intron 30.0%
GACACTTTGAATTGAAAAAG+TGG + chr4_3:58404844-58404863 Msa0651100:intron 30.0%
ACTCTTGGTTAAAACATGTT+AGG - chr4_3:58405044-58405063 None:intergenic 30.0%
AACATATAGTGAATGACTCT+TGG - chr4_3:58405059-58405078 None:intergenic 30.0%
! AGATCATAAGAGTTTTGCTT+TGG + chr4_3:58405264-58405283 Msa0651100:intron 30.0%
! AAGGAAATCATTTAGTTTCG+TGG + chr4_3:58405299-58405318 Msa0651100:intron 30.0%
! TTGGACAATTGATGTTTTCA+TGG + chr4_3:58405364-58405383 Msa0651100:CDS 30.0%
! ACAATTGATGTTTTCATGGA+TGG + chr4_3:58405368-58405387 Msa0651100:CDS 30.0%
TACGACATGTTACATTACTT+AGG - chr4_3:58405428-58405447 None:intergenic 30.0%
AGCCGACAATTAAGTTTATT+AGG + chr4_3:58405464-58405483 Msa0651100:three_prime_UTR 30.0%
TGGTGATTTATGTTATGACA+TGG + chr4_3:58405492-58405511 Msa0651100:three_prime_UTR 30.0%
GTTGAAAATGAAGGTTATTG+AGG - chr4_3:58405537-58405556 None:intergenic 30.0%
! AATAACCTTCATTTTCAACC+TGG + chr4_3:58405538-58405557 Msa0651100:three_prime_UTR 30.0%
!! ATTTTCCAGGTTGAAAATGA+AGG - chr4_3:58405546-58405565 None:intergenic 30.0%
! TCTAAAGACCAACGTGTTTT+GGG + chr4_3:58404180-58404199 Msa0651100:five_prime_UTR 35.0%
TATTACGTTCATTAGGAACC+CGG - chr4_3:58404229-58404248 None:intergenic 35.0%
! AGCATTGAAGAAAAAGGCAT+TGG + chr4_3:58404296-58404315 Msa0651100:CDS 35.0%
!! GAAGAAAAAGGCATTGGAAT+TGG + chr4_3:58404302-58404321 Msa0651100:CDS 35.0%
GAAAATTAGTCACTAATGGG+TGG + chr4_3:58404535-58404554 Msa0651100:intron 35.0%
CGAGAAAACTTGGATTCATA+CGG - chr4_3:58404701-58404720 None:intergenic 35.0%
!! TTTTTCTCCAGTTCTGATTC+TGG - chr4_3:58404732-58404751 None:intergenic 35.0%
AACTGGAGAAAAATTGCGTT+TGG + chr4_3:58404739-58404758 Msa0651100:CDS 35.0%
GGGAACTAATAGAATTTGAG+AGG + chr4_3:58405104-58405123 Msa0651100:intron 35.0%
TGTTATATGATGTCTACAGG+AGG + chr4_3:58405331-58405350 Msa0651100:intron 35.0%
! CTTAATTGTCGGCTAATACA+AGG - chr4_3:58405458-58405477 None:intergenic 35.0%
!!! AAGATATGCCATTTTGCATC+TGG - chr4_3:58405596-58405615 None:intergenic 35.0%
! TTTCAAGTATCCATGCTTCT+CGG - chr4_3:58405749-58405768 None:intergenic 35.0%
! TCTAAAGACCAACGTGTTTT+GGG + chr4_3:58404180-58404199 Msa0651100:five_prime_UTR 35.0%
TATTACGTTCATTAGGAACC+CGG - chr4_3:58404229-58404248 None:intergenic 35.0%
! AGCATTGAAGAAAAAGGCAT+TGG + chr4_3:58404296-58404315 Msa0651100:CDS 35.0%
!! GAAGAAAAAGGCATTGGAAT+TGG + chr4_3:58404302-58404321 Msa0651100:CDS 35.0%
GAAAATTAGTCACTAATGGG+TGG + chr4_3:58404535-58404554 Msa0651100:intron 35.0%
CGAGAAAACTTGGATTCATA+CGG - chr4_3:58404701-58404720 None:intergenic 35.0%
!! TTTTTCTCCAGTTCTGATTC+TGG - chr4_3:58404732-58404751 None:intergenic 35.0%
AACTGGAGAAAAATTGCGTT+TGG + chr4_3:58404739-58404758 Msa0651100:CDS 35.0%
GGGAACTAATAGAATTTGAG+AGG + chr4_3:58405104-58405123 Msa0651100:intron 35.0%
TGTTATATGATGTCTACAGG+AGG + chr4_3:58405331-58405350 Msa0651100:intron 35.0%
! CTTAATTGTCGGCTAATACA+AGG - chr4_3:58405458-58405477 None:intergenic 35.0%
!!! AAGATATGCCATTTTGCATC+TGG - chr4_3:58405596-58405615 None:intergenic 35.0%
! TTTCAAGTATCCATGCTTCT+CGG - chr4_3:58405749-58405768 None:intergenic 35.0%
!! CAAAACACGTTGGTCTTTAG+AGG - chr4_3:58404181-58404200 None:intergenic 40.0%
!! CTCTAAAGACCAACGTGTTT+TGG + chr4_3:58404179-58404198 Msa0651100:five_prime_UTR 40.0%
! CTAAAGACCAACGTGTTTTG+GGG + chr4_3:58404181-58404200 Msa0651100:five_prime_UTR 40.0%
! TTTTCTTCCCCAAAACACGT+TGG - chr4_3:58404191-58404210 None:intergenic 40.0%
CATTGGAATTGGAGAAGAAG+AGG + chr4_3:58404313-58404332 Msa0651100:CDS 40.0%
CAAAGACCAGTTCATCGTTA+CGG + chr4_3:58404356-58404375 Msa0651100:CDS 40.0%
TTTCGCCAAACTTCTCATGA+TGG + chr4_3:58404449-58404468 Msa0651100:CDS 40.0%
! TAGTCACTAATGGGTGGTAA+TGG + chr4_3:58404541-58404560 Msa0651100:intron 40.0%
AGGATAAAGAATGCTCCTGA+AGG + chr4_3:58404608-58404627 Msa0651100:CDS 40.0%
TTGGATTCATACGGAGTTCT+AGG - chr4_3:58404692-58404711 None:intergenic 40.0%
!! CCAGTTCTGATTCTGGAATT+GGG - chr4_3:58404725-58404744 None:intergenic 40.0%
!! TCCAGTTCTGATTCTGGAAT+TGG - chr4_3:58404726-58404745 None:intergenic 40.0%
TAACGCTGACACATAAACAC+CGG - chr4_3:58404944-58404963 None:intergenic 40.0%
GTCAGCGTTAGTGTTGTATT+CGG + chr4_3:58404954-58404973 Msa0651100:intron 40.0%
AACATGTTAGGTCTGCAAGT+GGG - chr4_3:58405032-58405051 None:intergenic 40.0%
AAACATGTTAGGTCTGCAAG+TGG - chr4_3:58405033-58405052 None:intergenic 40.0%
TTTACCATAGTCTTCAGCTC+TGG - chr4_3:58405399-58405418 None:intergenic 40.0%
GCTGAAGACTATGGTAAACA+TGG + chr4_3:58405401-58405420 Msa0651100:CDS 40.0%
! AAGAGTTCAGGCAATTTTCC+AGG - chr4_3:58405559-58405578 None:intergenic 40.0%
GGAAAATTGCCTGAACTCTT+TGG + chr4_3:58405559-58405578 Msa0651100:three_prime_UTR 40.0%
ACATGCAACCAGATGCAAAA+TGG + chr4_3:58405585-58405604 Msa0651100:three_prime_UTR 40.0%
TTCAATTTGACCGAGAAGCA+TGG + chr4_3:58405736-58405755 Msa0651100:three_prime_UTR 40.0%
!! CAAAACACGTTGGTCTTTAG+AGG - chr4_3:58404181-58404200 None:intergenic 40.0%
!! CTCTAAAGACCAACGTGTTT+TGG + chr4_3:58404179-58404198 Msa0651100:five_prime_UTR 40.0%
! CTAAAGACCAACGTGTTTTG+GGG + chr4_3:58404181-58404200 Msa0651100:five_prime_UTR 40.0%
! TTTTCTTCCCCAAAACACGT+TGG - chr4_3:58404191-58404210 None:intergenic 40.0%
CATTGGAATTGGAGAAGAAG+AGG + chr4_3:58404313-58404332 Msa0651100:CDS 40.0%
CAAAGACCAGTTCATCGTTA+CGG + chr4_3:58404356-58404375 Msa0651100:CDS 40.0%
TTTCGCCAAACTTCTCATGA+TGG + chr4_3:58404449-58404468 Msa0651100:CDS 40.0%
! TAGTCACTAATGGGTGGTAA+TGG + chr4_3:58404541-58404560 Msa0651100:intron 40.0%
AGGATAAAGAATGCTCCTGA+AGG + chr4_3:58404608-58404627 Msa0651100:CDS 40.0%
TTGGATTCATACGGAGTTCT+AGG - chr4_3:58404692-58404711 None:intergenic 40.0%
!! CCAGTTCTGATTCTGGAATT+GGG - chr4_3:58404725-58404744 None:intergenic 40.0%
!! TCCAGTTCTGATTCTGGAAT+TGG - chr4_3:58404726-58404745 None:intergenic 40.0%
TAACGCTGACACATAAACAC+CGG - chr4_3:58404944-58404963 None:intergenic 40.0%
GTCAGCGTTAGTGTTGTATT+CGG + chr4_3:58404954-58404973 Msa0651100:intron 40.0%
AACATGTTAGGTCTGCAAGT+GGG - chr4_3:58405032-58405051 None:intergenic 40.0%
AAACATGTTAGGTCTGCAAG+TGG - chr4_3:58405033-58405052 None:intergenic 40.0%
TTTACCATAGTCTTCAGCTC+TGG - chr4_3:58405399-58405418 None:intergenic 40.0%
GCTGAAGACTATGGTAAACA+TGG + chr4_3:58405401-58405420 Msa0651100:CDS 40.0%
! AAGAGTTCAGGCAATTTTCC+AGG - chr4_3:58405559-58405578 None:intergenic 40.0%
GGAAAATTGCCTGAACTCTT+TGG + chr4_3:58405559-58405578 Msa0651100:three_prime_UTR 40.0%
ACATGCAACCAGATGCAAAA+TGG + chr4_3:58405585-58405604 Msa0651100:three_prime_UTR 40.0%
TTCAATTTGACCGAGAAGCA+TGG + chr4_3:58405736-58405755 Msa0651100:three_prime_UTR 40.0%
AGATTGTTACACACACTGCG+TGG + chr4_3:58404255-58404274 Msa0651100:CDS 45.0%
TGGAGAAGAAGAGGAAGAAG+AGG + chr4_3:58404322-58404341 Msa0651100:CDS 45.0%
GGAGAAGAAGAGGAAGAAGA+GGG + chr4_3:58404323-58404342 Msa0651100:CDS 45.0%
!! CGTAACGATGAACTGGTCTT+TGG - chr4_3:58404358-58404377 None:intergenic 45.0%
CGAGGTAGGATAAGGATTCA+GGG - chr4_3:58404383-58404402 None:intergenic 45.0%
TCGAGGTAGGATAAGGATTC+AGG - chr4_3:58404384-58404403 None:intergenic 45.0%
ATCCAGATCCCAAGAGTTGT+TGG + chr4_3:58404580-58404599 Msa0651100:CDS 45.0%
AAGAATGCTCCTGAAGGTCA+AGG + chr4_3:58404614-58404633 Msa0651100:CDS 45.0%
GGAATTGGGACGAGAAAACT+TGG - chr4_3:58404711-58404730 None:intergenic 45.0%
CCCAATTCCAGAATCAGAAC+TGG + chr4_3:58404722-58404741 Msa0651100:CDS 45.0%
TCATCAGCATTGTCTGTCTC+TGG - chr4_3:58404811-58404830 None:intergenic 45.0%
TGCTGATGACCTATGTAGCT+CGG + chr4_3:58404822-58404841 Msa0651100:intron 45.0%
! TCAAAGTGTCCGAGCTACAT+AGG - chr4_3:58404834-58404853 None:intergenic 45.0%
TCGTGTGTGTGTCAAGTGAA+AGG - chr4_3:58405231-58405250 None:intergenic 45.0%
ATGTCTACAGGAGGACGTAA+AGG + chr4_3:58405340-58405359 Msa0651100:intron 45.0%
TACAGGAGGACGTAAAGGAT+TGG + chr4_3:58405345-58405364 Msa0651100:intron 45.0%
GCATGTTTGCCAAAGAGTTC+AGG - chr4_3:58405571-58405590 None:intergenic 45.0%
AGATTGTTACACACACTGCG+TGG + chr4_3:58404255-58404274 Msa0651100:CDS 45.0%
TGGAGAAGAAGAGGAAGAAG+AGG + chr4_3:58404322-58404341 Msa0651100:CDS 45.0%
GGAGAAGAAGAGGAAGAAGA+GGG + chr4_3:58404323-58404342 Msa0651100:CDS 45.0%
!! CGTAACGATGAACTGGTCTT+TGG - chr4_3:58404358-58404377 None:intergenic 45.0%
CGAGGTAGGATAAGGATTCA+GGG - chr4_3:58404383-58404402 None:intergenic 45.0%
TCGAGGTAGGATAAGGATTC+AGG - chr4_3:58404384-58404403 None:intergenic 45.0%
ATCCAGATCCCAAGAGTTGT+TGG + chr4_3:58404580-58404599 Msa0651100:CDS 45.0%
AAGAATGCTCCTGAAGGTCA+AGG + chr4_3:58404614-58404633 Msa0651100:CDS 45.0%
GGAATTGGGACGAGAAAACT+TGG - chr4_3:58404711-58404730 None:intergenic 45.0%
CCCAATTCCAGAATCAGAAC+TGG + chr4_3:58404722-58404741 Msa0651100:CDS 45.0%
TCATCAGCATTGTCTGTCTC+TGG - chr4_3:58404811-58404830 None:intergenic 45.0%
TGCTGATGACCTATGTAGCT+CGG + chr4_3:58404822-58404841 Msa0651100:intron 45.0%
! TCAAAGTGTCCGAGCTACAT+AGG - chr4_3:58404834-58404853 None:intergenic 45.0%
TCGTGTGTGTGTCAAGTGAA+AGG - chr4_3:58405231-58405250 None:intergenic 45.0%
ATGTCTACAGGAGGACGTAA+AGG + chr4_3:58405340-58405359 Msa0651100:intron 45.0%
TACAGGAGGACGTAAAGGAT+TGG + chr4_3:58405345-58405364 Msa0651100:intron 45.0%
GCATGTTTGCCAAAGAGTTC+AGG - chr4_3:58405571-58405590 None:intergenic 45.0%
CCAACAGCAATGCCGATCAT+GGG - chr4_3:58404420-58404439 None:intergenic 50.0%
CTCCAACAACTCTTGGGATC+TGG - chr4_3:58404585-58404604 None:intergenic 50.0%
CCTACGCTCCAACAACTCTT+GGG - chr4_3:58404591-58404610 None:intergenic 50.0%
TCCTACGCTCCAACAACTCT+TGG - chr4_3:58404592-58404611 None:intergenic 50.0%
CTCACAGTACCTTGACCTTC+AGG - chr4_3:58404626-58404645 None:intergenic 50.0%
AATGCTCAGCCGTGAAGAGT+GGG + chr4_3:58404646-58404665 Msa0651100:CDS 50.0%
GAACCTTGTCCCACTCTTCA+CGG - chr4_3:58404658-58404677 None:intergenic 50.0%
GTTGCCAGAGCTGAAGACTA+TGG + chr4_3:58405392-58405411 Msa0651100:CDS 50.0%
CCAACAGCAATGCCGATCAT+GGG - chr4_3:58404420-58404439 None:intergenic 50.0%
CTCCAACAACTCTTGGGATC+TGG - chr4_3:58404585-58404604 None:intergenic 50.0%
CCTACGCTCCAACAACTCTT+GGG - chr4_3:58404591-58404610 None:intergenic 50.0%
TCCTACGCTCCAACAACTCT+TGG - chr4_3:58404592-58404611 None:intergenic 50.0%
CTCACAGTACCTTGACCTTC+AGG - chr4_3:58404626-58404645 None:intergenic 50.0%
AATGCTCAGCCGTGAAGAGT+GGG + chr4_3:58404646-58404665 Msa0651100:CDS 50.0%
GAACCTTGTCCCACTCTTCA+CGG - chr4_3:58404658-58404677 None:intergenic 50.0%
GTTGCCAGAGCTGAAGACTA+TGG + chr4_3:58405392-58405411 Msa0651100:CDS 50.0%
CAGGGACCGTAACGATGAAC+TGG - chr4_3:58404365-58404384 None:intergenic 55.0%
! TGCGGTGTCGAGGTAGGATA+AGG - chr4_3:58404391-58404410 None:intergenic 55.0%
ACCGCATCTCTGCCCATGAT+CGG + chr4_3:58404405-58404424 Msa0651100:CDS 55.0%
CCCATGATCGGCATTGCTGT+TGG + chr4_3:58404417-58404436 Msa0651100:CDS 55.0%
GCCAACAGCAATGCCGATCA+TGG - chr4_3:58404421-58404440 None:intergenic 55.0%
CCCAAGAGTTGTTGGAGCGT+AGG + chr4_3:58404588-58404607 Msa0651100:CDS 55.0%
GAATGCTCAGCCGTGAAGAG+TGG + chr4_3:58404645-58404664 Msa0651100:CDS 55.0%
GGGACAAGGTTCGAGAACTC+AGG + chr4_3:58404666-58404685 Msa0651100:CDS 55.0%
CAGGGACCGTAACGATGAAC+TGG - chr4_3:58404365-58404384 None:intergenic 55.0%
! TGCGGTGTCGAGGTAGGATA+AGG - chr4_3:58404391-58404410 None:intergenic 55.0%
ACCGCATCTCTGCCCATGAT+CGG + chr4_3:58404405-58404424 Msa0651100:CDS 55.0%
CCCATGATCGGCATTGCTGT+TGG + chr4_3:58404417-58404436 Msa0651100:CDS 55.0%
GCCAACAGCAATGCCGATCA+TGG - chr4_3:58404421-58404440 None:intergenic 55.0%
CCCAAGAGTTGTTGGAGCGT+AGG + chr4_3:58404588-58404607 Msa0651100:CDS 55.0%
GAATGCTCAGCCGTGAAGAG+TGG + chr4_3:58404645-58404664 Msa0651100:CDS 55.0%
GGGACAAGGTTCGAGAACTC+AGG + chr4_3:58404666-58404685 Msa0651100:CDS 55.0%
! CAGAGATGCGGTGTCGAGGT+AGG - chr4_3:58404397-58404416 None:intergenic 60.0%
GCCGATCATGGGCAGAGATG+CGG - chr4_3:58404409-58404428 None:intergenic 60.0%
CAGCCGTGAAGAGTGGGACA+AGG + chr4_3:58404652-58404671 Msa0651100:CDS 60.0%
! CAGAGATGCGGTGTCGAGGT+AGG - chr4_3:58404397-58404416 None:intergenic 60.0%
GCCGATCATGGGCAGAGATG+CGG - chr4_3:58404409-58404428 None:intergenic 60.0%
CAGCCGTGAAGAGTGGGACA+AGG + chr4_3:58404652-58404671 Msa0651100:CDS 60.0%
! TGGGCAGAGATGCGGTGTCG+AGG - chr4_3:58404401-58404420 None:intergenic 65.0%
! TGGGCAGAGATGCGGTGTCG+AGG - chr4_3:58404401-58404420 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 58404170 58405790 58404170 ID=Msa0651100;Name=Msa0651100
chr4_3 mRNA 58404170 58405790 58404170 ID=Msa0651100-mRNA-1;Parent=Msa0651100;Name=Msa0651100-mRNA-1;_AED=0.01;_eAED=0.01;_QI=67|1|1|1|1|1|3|357|168
chr4_3 exon 58404170 58404470 58404170 ID=Msa0651100-mRNA-1:exon:15454;Parent=Msa0651100-mRNA-1
chr4_3 exon 58404572 58404760 58404572 ID=Msa0651100-mRNA-1:exon:15455;Parent=Msa0651100-mRNA-1
chr4_3 exon 58405350 58405790 58405350 ID=Msa0651100-mRNA-1:exon:15456;Parent=Msa0651100-mRNA-1
chr4_3 five_prime_UTR 58404170 58404236 58404170 ID=Msa0651100-mRNA-1:five_prime_utr;Parent=Msa0651100-mRNA-1
chr4_3 CDS 58404237 58404470 58404237 ID=Msa0651100-mRNA-1:cds;Parent=Msa0651100-mRNA-1
chr4_3 CDS 58404572 58404760 58404572 ID=Msa0651100-mRNA-1:cds;Parent=Msa0651100-mRNA-1
chr4_3 CDS 58405350 58405433 58405350 ID=Msa0651100-mRNA-1:cds;Parent=Msa0651100-mRNA-1
chr4_3 three_prime_UTR 58405434 58405790 58405434 ID=Msa0651100-mRNA-1:three_prime_utr;Parent=Msa0651100-mRNA-1
Gene Sequence

>Msa0651100

ATGAACGTAATAAGACAAAGATTGTTACACACACTGCGTGGCGATGTCTTCCCATCTGAAGCATTGAAGAAAAAGGCATTGGAATTGGAGAAGAAGAGGAAGAAGAGGGCTTCTAAGACCAAAGACCAGTTCATCGTTACGGTCCCTGAATCCTTATCCTACCTCGACACCGCATCTCTGCCCATGATCGGCATTGCTGTTGGCATTGCAGTTTTCGCCAAACTTCTCATGATGTTAGATGAATCCAGATCCCAAGAGTTGTTGGAGCGTAGGATAAAGAATGCTCCTGAAGGTCAAGGTACTGTGAGAATGCTCAGCCGTGAAGAGTGGGACAAGGTTCGAGAACTCAGGCCTAGAACTCCGTATGAATCCAAGTTTTCTCGTCCCAATTCCAGAATCAGAACTGGAGAAAAATTGCGTTTGGAGGACGTAAAGGATTGGACAATTGATGTTTTCATGGATGGTGTTGCCAGAGCTGAAGACTATGGTAAACATGGTCCTAAGTAA
Protein sequence

>Msa0651100

MNVIRQRLLHTLRGDVFPSEALKKKALELEKKRKKRASKTKDQFIVTVPESLSYLDTASLPMIGIAVGIAVFAKLLMMLDESRSQELLERRIKNAPEGQGTVRMLSREEWDKVRELRPRTPYESKFSRPNSRIRTGEKLRLEDVKDWTIDVFMDGVARAEDYGKHGPK*