AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610450 MtrunA17_Chr4g0043481 97.041 169 4 1 1 168 1 169 4.05e-118 330
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610450 AT2G04340.1 56.522 161 68 2 6 166 13 171 3.30e-64 195

Find 66 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAAAGACCAACGTGTTT+TGG 0.194073 4_2:+58159353 Msa0610450:five_prime_UTR
TAACTAGTGGTTTATACTTA+TGG 0.211874 4_2:+58160833 Msa0610450:three_prime_UTR
TCCAGTTCTGATTCTGGAAT+TGG 0.212325 4_2:-58159897 None:intergenic
TTGGATTCATACGGAGTTCT+AGG 0.245059 4_2:-58159863 None:intergenic
GGAAAATTGCCTGAACTCTT+TGG 0.253591 4_2:+58160733 Msa0610450:three_prime_UTR
AGCCGACAATTAAGTTTATT+AGG 0.286121 4_2:+58160638 Msa0610450:three_prime_UTR
TCGAGGTAGGATAAGGATTC+AGG 0.290716 4_2:-58159555 None:intergenic
AAAAGAAATCAAACATAAAA+AGG 0.290843 4_2:-58160871 None:intergenic
CAAAACACGTTGGTCTTTAG+AGG 0.314898 4_2:-58159352 None:intergenic
ATGCACTCTTAAGAAAATAA+AGG 0.322223 4_2:+58160809 Msa0610450:three_prime_UTR
AACTAGTGGTTTATACTTAT+GGG 0.322469 4_2:+58160834 Msa0610450:three_prime_UTR
ATCTGAAGCATTGAAGAAAA+AGG 0.323646 4_2:+58159464 Msa0610450:CDS
CTCACAGTACCTTGACCTTC+AGG 0.325447 4_2:-58159797 None:intergenic
TTTGTCTTATTACGTTCATT+AGG 0.328633 4_2:-58159407 None:intergenic
CCAGTTCTGATTCTGGAATT+GGG 0.353599 4_2:-58159896 None:intergenic
ATTTACCATCATGAGAAGTT+TGG 0.355493 4_2:-58159628 None:intergenic
TCCTACGCTCCAACAACTCT+TGG 0.371569 4_2:-58159763 None:intergenic
ACATGCAACCAGATGCAAAA+TGG 0.373896 4_2:+58160759 Msa0610450:three_prime_UTR
GCATGTTTGCCAAAGAGTTC+AGG 0.380995 4_2:-58160742 None:intergenic
CGTAACGATGAACTGGTCTT+TGG 0.392657 4_2:-58159529 None:intergenic
CACCTAATAAACTTAATTGT+CGG 0.406028 4_2:-58160640 None:intergenic
CTCCAACAACTCTTGGGATC+TGG 0.424915 4_2:-58159756 None:intergenic
ATCCAGATCCCAAGAGTTGT+TGG 0.426994 4_2:+58159754 Msa0610450:CDS
AAAACAACAATCAATCAAAC+CGG 0.431424 4_2:+58159381 Msa0610450:five_prime_UTR
GGAATTGGGACGAGAAAACT+TGG 0.437414 4_2:-58159882 None:intergenic
GCCAACAGCAATGCCGATCA+TGG 0.451912 4_2:-58159592 None:intergenic
CCCAATTCCAGAATCAGAAC+TGG 0.465325 4_2:+58159896 Msa0610450:CDS
GTTGAAAATGAAGGTTATTG+AGG 0.470958 4_2:-58160708 None:intergenic
TGCGGTGTCGAGGTAGGATA+AGG 0.473913 4_2:-58159562 None:intergenic
TGGAGAAGAAGAGGAAGAAG+AGG 0.480327 4_2:+58159496 Msa0610450:CDS
TTTCGCCAAACTTCTCATGA+TGG 0.487048 4_2:+58159623 Msa0610450:CDS
CAGGGACCGTAACGATGAAC+TGG 0.487342 4_2:-58159536 None:intergenic
GAACCTTGTCCCACTCTTCA+CGG 0.488340 4_2:-58159829 None:intergenic
ATTAAGTTTATTAGGTGTTG+TGG 0.490415 4_2:+58160646 Msa0610450:three_prime_UTR
CCTACGCTCCAACAACTCTT+GGG 0.506302 4_2:-58159762 None:intergenic
CTTAATTGTCGGCTAATACA+AGG 0.515960 4_2:-58160629 None:intergenic
TACGACATGTTACATTACTT+AGG 0.517159 4_2:-58160599 None:intergenic
TTTACCATAGTCTTCAGCTC+TGG 0.519072 4_2:-58160570 None:intergenic
CATTGGAATTGGAGAAGAAG+AGG 0.524044 4_2:+58159487 Msa0610450:CDS
CCCATGATCGGCATTGCTGT+TGG 0.525769 4_2:+58159591 Msa0610450:CDS
CGAGGTAGGATAAGGATTCA+GGG 0.534228 4_2:-58159554 None:intergenic
GTTGCCAGAGCTGAAGACTA+TGG 0.536885 4_2:+58160566 Msa0610450:CDS
ACCGCATCTCTGCCCATGAT+CGG 0.540473 4_2:+58159579 Msa0610450:CDS
TACAGGAGGACGTAAAGGAT+TGG 0.546228 4_2:+58160519 Msa0610450:intron
GGGACAAGGTTCGAGAACTC+AGG 0.548800 4_2:+58159840 Msa0610450:CDS
TTCAATTTGATCGAGAAGCA+TGG 0.549934 4_2:+58160910 Msa0610450:three_prime_UTR
CAAAGACCAGTTCATCGTTA+CGG 0.551542 4_2:+58159530 Msa0610450:CDS
GGAGAAGAAGAGGAAGAAGA+GGG 0.551810 4_2:+58159497 Msa0610450:CDS
CAGAGATGCGGTGTCGAGGT+AGG 0.553355 4_2:-58159568 None:intergenic
ATGTCTACAGGAGGACGTAA+AGG 0.556570 4_2:+58160514 Msa0610450:intron
CGAGAAAACTTGGATTCATA+CGG 0.560787 4_2:-58159872 None:intergenic
GAATGCTCAGCCGTGAAGAG+TGG 0.566528 4_2:+58159819 Msa0610450:CDS
AATGCTCAGCCGTGAAGAGT+GGG 0.573652 4_2:+58159820 Msa0610450:CDS
TGGTGATTTATGTTATGACA+TGG 0.576344 4_2:+58160666 Msa0610450:three_prime_UTR
CCAACAGCAATGCCGATCAT+GGG 0.576515 4_2:-58159591 None:intergenic
CCCAAGAGTTGTTGGAGCGT+AGG 0.581873 4_2:+58159762 Msa0610450:CDS
AGAAAATAAAGGATAACTAG+TGG 0.593663 4_2:+58160820 Msa0610450:three_prime_UTR
TATTACGTTCATTAGGAACC+CGG 0.599628 4_2:-58159400 None:intergenic
GCCGATCATGGGCAGAGATG+CGG 0.599977 4_2:-58159580 None:intergenic
AGGATAAAGAATGCTCCTGA+AGG 0.601200 4_2:+58159782 Msa0610450:CDS
AAGAATGCTCCTGAAGGTCA+AGG 0.605809 4_2:+58159788 Msa0610450:CDS
TGGGCAGAGATGCGGTGTCG+AGG 0.615374 4_2:-58159572 None:intergenic
CAGCCGTGAAGAGTGGGACA+AGG 0.626546 4_2:+58159826 Msa0610450:CDS
GCTGAAGACTATGGTAAACA+TGG 0.649911 4_2:+58160575 Msa0610450:CDS
AAACAACAATCAATCAAACC+GGG 0.669116 4_2:+58159382 Msa0610450:five_prime_UTR
AGATTGTTACACACACTGCG+TGG 0.702041 4_2:+58159429 Msa0610450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATTTTTAAAATTATTAC+CGG + chr4_2:58160096-58160115 Msa0610450:intron 10.0%
!!! TCTATTTTTAAAATTATTAC+CGG + chr4_2:58160096-58160115 Msa0610450:intron 10.0%
!!! AATTTTAAAAATAGAAGTGA+TGG - chr4_2:58160091-58160110 None:intergenic 15.0%
!! AAAAGAAATCAAACATAAAA+AGG - chr4_2:58160874-58160893 None:intergenic 15.0%
!!! AATTTTAAAAATAGAAGTGA+TGG - chr4_2:58160091-58160110 None:intergenic 15.0%
!! AAAAGAAATCAAACATAAAA+AGG - chr4_2:58160874-58160893 None:intergenic 15.0%
!! TTTCTTTGTTCATTGTATAT+AGG + chr4_2:58160257-58160276 Msa0610450:intron 20.0%
!! TTCTTTGTTCATTGTATATA+GGG + chr4_2:58160258-58160277 Msa0610450:intron 20.0%
!! TTTCTTTGTTCATTGTATAT+AGG + chr4_2:58160257-58160276 Msa0610450:intron 20.0%
!! TTCTTTGTTCATTGTATATA+GGG + chr4_2:58160258-58160277 Msa0610450:intron 20.0%
! AAAACAACAATCAATCAAAC+CGG + chr4_2:58159381-58159400 Msa0610450:five_prime_UTR 25.0%
!! TTTGTCTTATTACGTTCATT+AGG - chr4_2:58159410-58159429 None:intergenic 25.0%
! AGTGAAAATTAGTCACTAAT+GGG + chr4_2:58159706-58159725 Msa0610450:intron 25.0%
! ATGTAGTAATTCTACATGAT+TGG + chr4_2:58160316-58160335 Msa0610450:intron 25.0%
!!! GCTTTGGAATATATAATACA+AGG + chr4_2:58160454-58160473 Msa0610450:intron 25.0%
!!! TTTTGTTATATGATGTCTAC+AGG + chr4_2:58160502-58160521 Msa0610450:intron 25.0%
! CACCTAATAAACTTAATTGT+CGG - chr4_2:58160643-58160662 None:intergenic 25.0%
!! ATTAAGTTTATTAGGTGTTG+TGG + chr4_2:58160646-58160665 Msa0610450:three_prime_UTR 25.0%
! ATGCACTCTTAAGAAAATAA+AGG + chr4_2:58160809-58160828 Msa0610450:three_prime_UTR 25.0%
! AGAAAATAAAGGATAACTAG+TGG + chr4_2:58160820-58160839 Msa0610450:three_prime_UTR 25.0%
! TAACTAGTGGTTTATACTTA+TGG + chr4_2:58160833-58160852 Msa0610450:three_prime_UTR 25.0%
! AACTAGTGGTTTATACTTAT+GGG + chr4_2:58160834-58160853 Msa0610450:three_prime_UTR 25.0%
! AAAACAACAATCAATCAAAC+CGG + chr4_2:58159381-58159400 Msa0610450:five_prime_UTR 25.0%
!! TTTGTCTTATTACGTTCATT+AGG - chr4_2:58159410-58159429 None:intergenic 25.0%
! AGTGAAAATTAGTCACTAAT+GGG + chr4_2:58159706-58159725 Msa0610450:intron 25.0%
! ATGTAGTAATTCTACATGAT+TGG + chr4_2:58160316-58160335 Msa0610450:intron 25.0%
!!! GCTTTGGAATATATAATACA+AGG + chr4_2:58160454-58160473 Msa0610450:intron 25.0%
!!! TTTTGTTATATGATGTCTAC+AGG + chr4_2:58160502-58160521 Msa0610450:intron 25.0%
! CACCTAATAAACTTAATTGT+CGG - chr4_2:58160643-58160662 None:intergenic 25.0%
!! ATTAAGTTTATTAGGTGTTG+TGG + chr4_2:58160646-58160665 Msa0610450:three_prime_UTR 25.0%
! ATGCACTCTTAAGAAAATAA+AGG + chr4_2:58160809-58160828 Msa0610450:three_prime_UTR 25.0%
! AGAAAATAAAGGATAACTAG+TGG + chr4_2:58160820-58160839 Msa0610450:three_prime_UTR 25.0%
! TAACTAGTGGTTTATACTTA+TGG + chr4_2:58160833-58160852 Msa0610450:three_prime_UTR 25.0%
! AACTAGTGGTTTATACTTAT+GGG + chr4_2:58160834-58160853 Msa0610450:three_prime_UTR 25.0%
AAACAACAATCAATCAAACC+GGG + chr4_2:58159382-58159401 Msa0610450:five_prime_UTR 30.0%
! TTTTCTTCAATGCTTCAGAT+GGG - chr4_2:58159464-58159483 None:intergenic 30.0%
! TTTTTCTTCAATGCTTCAGA+TGG - chr4_2:58159465-58159484 None:intergenic 30.0%
!! ATCTGAAGCATTGAAGAAAA+AGG + chr4_2:58159464-58159483 Msa0610450:CDS 30.0%
ATTTACCATCATGAGAAGTT+TGG - chr4_2:58159631-58159650 None:intergenic 30.0%
CAGTGAAAATTAGTCACTAA+TGG + chr4_2:58159705-58159724 Msa0610450:intron 30.0%
GACACTTTGAATTGAAAAAG+TGG + chr4_2:58160018-58160037 Msa0610450:intron 30.0%
ACTCTTGGTTAAAACATGTT+AGG - chr4_2:58160218-58160237 None:intergenic 30.0%
AACATATAGTGAATGACTCT+TGG - chr4_2:58160233-58160252 None:intergenic 30.0%
! AGATCATAAGAGTTTTGCTT+TGG + chr4_2:58160438-58160457 Msa0610450:intron 30.0%
! AAGGAAATCATTTAGTTTCG+TGG + chr4_2:58160473-58160492 Msa0610450:intron 30.0%
! TTGGACAATTGATGTTTTCA+TGG + chr4_2:58160538-58160557 Msa0610450:CDS 30.0%
! ACAATTGATGTTTTCATGGA+TGG + chr4_2:58160542-58160561 Msa0610450:CDS 30.0%
TACGACATGTTACATTACTT+AGG - chr4_2:58160602-58160621 None:intergenic 30.0%
AGCCGACAATTAAGTTTATT+AGG + chr4_2:58160638-58160657 Msa0610450:three_prime_UTR 30.0%
TGGTGATTTATGTTATGACA+TGG + chr4_2:58160666-58160685 Msa0610450:three_prime_UTR 30.0%
GTTGAAAATGAAGGTTATTG+AGG - chr4_2:58160711-58160730 None:intergenic 30.0%
! AATAACCTTCATTTTCAACC+TGG + chr4_2:58160712-58160731 Msa0610450:three_prime_UTR 30.0%
!! ATTTTCCAGGTTGAAAATGA+AGG - chr4_2:58160720-58160739 None:intergenic 30.0%
AAACAACAATCAATCAAACC+GGG + chr4_2:58159382-58159401 Msa0610450:five_prime_UTR 30.0%
! TTTTCTTCAATGCTTCAGAT+GGG - chr4_2:58159464-58159483 None:intergenic 30.0%
! TTTTTCTTCAATGCTTCAGA+TGG - chr4_2:58159465-58159484 None:intergenic 30.0%
!! ATCTGAAGCATTGAAGAAAA+AGG + chr4_2:58159464-58159483 Msa0610450:CDS 30.0%
ATTTACCATCATGAGAAGTT+TGG - chr4_2:58159631-58159650 None:intergenic 30.0%
CAGTGAAAATTAGTCACTAA+TGG + chr4_2:58159705-58159724 Msa0610450:intron 30.0%
GACACTTTGAATTGAAAAAG+TGG + chr4_2:58160018-58160037 Msa0610450:intron 30.0%
ACTCTTGGTTAAAACATGTT+AGG - chr4_2:58160218-58160237 None:intergenic 30.0%
AACATATAGTGAATGACTCT+TGG - chr4_2:58160233-58160252 None:intergenic 30.0%
! AGATCATAAGAGTTTTGCTT+TGG + chr4_2:58160438-58160457 Msa0610450:intron 30.0%
! AAGGAAATCATTTAGTTTCG+TGG + chr4_2:58160473-58160492 Msa0610450:intron 30.0%
! TTGGACAATTGATGTTTTCA+TGG + chr4_2:58160538-58160557 Msa0610450:CDS 30.0%
! ACAATTGATGTTTTCATGGA+TGG + chr4_2:58160542-58160561 Msa0610450:CDS 30.0%
TACGACATGTTACATTACTT+AGG - chr4_2:58160602-58160621 None:intergenic 30.0%
AGCCGACAATTAAGTTTATT+AGG + chr4_2:58160638-58160657 Msa0610450:three_prime_UTR 30.0%
TGGTGATTTATGTTATGACA+TGG + chr4_2:58160666-58160685 Msa0610450:three_prime_UTR 30.0%
GTTGAAAATGAAGGTTATTG+AGG - chr4_2:58160711-58160730 None:intergenic 30.0%
! AATAACCTTCATTTTCAACC+TGG + chr4_2:58160712-58160731 Msa0610450:three_prime_UTR 30.0%
!! ATTTTCCAGGTTGAAAATGA+AGG - chr4_2:58160720-58160739 None:intergenic 30.0%
! TCTAAAGACCAACGTGTTTT+GGG + chr4_2:58159354-58159373 Msa0610450:five_prime_UTR 35.0%
TATTACGTTCATTAGGAACC+CGG - chr4_2:58159403-58159422 None:intergenic 35.0%
! AGCATTGAAGAAAAAGGCAT+TGG + chr4_2:58159470-58159489 Msa0610450:CDS 35.0%
!! GAAGAAAAAGGCATTGGAAT+TGG + chr4_2:58159476-58159495 Msa0610450:CDS 35.0%
GAAAATTAGTCACTAATGGG+TGG + chr4_2:58159709-58159728 Msa0610450:intron 35.0%
CGAGAAAACTTGGATTCATA+CGG - chr4_2:58159875-58159894 None:intergenic 35.0%
!! TTTTTCTCCAGTTCTGATTC+TGG - chr4_2:58159906-58159925 None:intergenic 35.0%
AACTGGAGAAAAATTGCGTT+TGG + chr4_2:58159913-58159932 Msa0610450:CDS 35.0%
GGGAACTAATAGAATTTGAG+AGG + chr4_2:58160278-58160297 Msa0610450:intron 35.0%
TGTTATATGATGTCTACAGG+AGG + chr4_2:58160505-58160524 Msa0610450:intron 35.0%
! CTTAATTGTCGGCTAATACA+AGG - chr4_2:58160632-58160651 None:intergenic 35.0%
!!! AAGATATGCCATTTTGCATC+TGG - chr4_2:58160770-58160789 None:intergenic 35.0%
TTCAATTTGATCGAGAAGCA+TGG + chr4_2:58160910-58160929 Msa0610450:three_prime_UTR 35.0%
! TCTAAAGACCAACGTGTTTT+GGG + chr4_2:58159354-58159373 Msa0610450:five_prime_UTR 35.0%
TATTACGTTCATTAGGAACC+CGG - chr4_2:58159403-58159422 None:intergenic 35.0%
! AGCATTGAAGAAAAAGGCAT+TGG + chr4_2:58159470-58159489 Msa0610450:CDS 35.0%
!! GAAGAAAAAGGCATTGGAAT+TGG + chr4_2:58159476-58159495 Msa0610450:CDS 35.0%
GAAAATTAGTCACTAATGGG+TGG + chr4_2:58159709-58159728 Msa0610450:intron 35.0%
CGAGAAAACTTGGATTCATA+CGG - chr4_2:58159875-58159894 None:intergenic 35.0%
!! TTTTTCTCCAGTTCTGATTC+TGG - chr4_2:58159906-58159925 None:intergenic 35.0%
AACTGGAGAAAAATTGCGTT+TGG + chr4_2:58159913-58159932 Msa0610450:CDS 35.0%
GGGAACTAATAGAATTTGAG+AGG + chr4_2:58160278-58160297 Msa0610450:intron 35.0%
TGTTATATGATGTCTACAGG+AGG + chr4_2:58160505-58160524 Msa0610450:intron 35.0%
! CTTAATTGTCGGCTAATACA+AGG - chr4_2:58160632-58160651 None:intergenic 35.0%
!!! AAGATATGCCATTTTGCATC+TGG - chr4_2:58160770-58160789 None:intergenic 35.0%
TTCAATTTGATCGAGAAGCA+TGG + chr4_2:58160910-58160929 Msa0610450:three_prime_UTR 35.0%
!! CAAAACACGTTGGTCTTTAG+AGG - chr4_2:58159355-58159374 None:intergenic 40.0%
!! CTCTAAAGACCAACGTGTTT+TGG + chr4_2:58159353-58159372 Msa0610450:five_prime_UTR 40.0%
! CTAAAGACCAACGTGTTTTG+GGG + chr4_2:58159355-58159374 Msa0610450:five_prime_UTR 40.0%
! TTTTCTTCCCCAAAACACGT+TGG - chr4_2:58159365-58159384 None:intergenic 40.0%
CATTGGAATTGGAGAAGAAG+AGG + chr4_2:58159487-58159506 Msa0610450:CDS 40.0%
CAAAGACCAGTTCATCGTTA+CGG + chr4_2:58159530-58159549 Msa0610450:CDS 40.0%
TTTCGCCAAACTTCTCATGA+TGG + chr4_2:58159623-58159642 Msa0610450:CDS 40.0%
! TAGTCACTAATGGGTGGTAA+TGG + chr4_2:58159715-58159734 Msa0610450:intron 40.0%
AGGATAAAGAATGCTCCTGA+AGG + chr4_2:58159782-58159801 Msa0610450:CDS 40.0%
TTGGATTCATACGGAGTTCT+AGG - chr4_2:58159866-58159885 None:intergenic 40.0%
!! CCAGTTCTGATTCTGGAATT+GGG - chr4_2:58159899-58159918 None:intergenic 40.0%
!! TCCAGTTCTGATTCTGGAAT+TGG - chr4_2:58159900-58159919 None:intergenic 40.0%
TAACGCTGACACATAAACAC+CGG - chr4_2:58160118-58160137 None:intergenic 40.0%
GTCAGCGTTAGTGTTGTATT+CGG + chr4_2:58160128-58160147 Msa0610450:intron 40.0%
AACATGTTAGGTCTGCAAGT+GGG - chr4_2:58160206-58160225 None:intergenic 40.0%
AAACATGTTAGGTCTGCAAG+TGG - chr4_2:58160207-58160226 None:intergenic 40.0%
TTTACCATAGTCTTCAGCTC+TGG - chr4_2:58160573-58160592 None:intergenic 40.0%
GCTGAAGACTATGGTAAACA+TGG + chr4_2:58160575-58160594 Msa0610450:CDS 40.0%
! AAGAGTTCAGGCAATTTTCC+AGG - chr4_2:58160733-58160752 None:intergenic 40.0%
GGAAAATTGCCTGAACTCTT+TGG + chr4_2:58160733-58160752 Msa0610450:three_prime_UTR 40.0%
ACATGCAACCAGATGCAAAA+TGG + chr4_2:58160759-58160778 Msa0610450:three_prime_UTR 40.0%
!! CAAAACACGTTGGTCTTTAG+AGG - chr4_2:58159355-58159374 None:intergenic 40.0%
!! CTCTAAAGACCAACGTGTTT+TGG + chr4_2:58159353-58159372 Msa0610450:five_prime_UTR 40.0%
! CTAAAGACCAACGTGTTTTG+GGG + chr4_2:58159355-58159374 Msa0610450:five_prime_UTR 40.0%
! TTTTCTTCCCCAAAACACGT+TGG - chr4_2:58159365-58159384 None:intergenic 40.0%
CATTGGAATTGGAGAAGAAG+AGG + chr4_2:58159487-58159506 Msa0610450:CDS 40.0%
CAAAGACCAGTTCATCGTTA+CGG + chr4_2:58159530-58159549 Msa0610450:CDS 40.0%
TTTCGCCAAACTTCTCATGA+TGG + chr4_2:58159623-58159642 Msa0610450:CDS 40.0%
! TAGTCACTAATGGGTGGTAA+TGG + chr4_2:58159715-58159734 Msa0610450:intron 40.0%
AGGATAAAGAATGCTCCTGA+AGG + chr4_2:58159782-58159801 Msa0610450:CDS 40.0%
TTGGATTCATACGGAGTTCT+AGG - chr4_2:58159866-58159885 None:intergenic 40.0%
!! CCAGTTCTGATTCTGGAATT+GGG - chr4_2:58159899-58159918 None:intergenic 40.0%
!! TCCAGTTCTGATTCTGGAAT+TGG - chr4_2:58159900-58159919 None:intergenic 40.0%
TAACGCTGACACATAAACAC+CGG - chr4_2:58160118-58160137 None:intergenic 40.0%
GTCAGCGTTAGTGTTGTATT+CGG + chr4_2:58160128-58160147 Msa0610450:intron 40.0%
AACATGTTAGGTCTGCAAGT+GGG - chr4_2:58160206-58160225 None:intergenic 40.0%
AAACATGTTAGGTCTGCAAG+TGG - chr4_2:58160207-58160226 None:intergenic 40.0%
TTTACCATAGTCTTCAGCTC+TGG - chr4_2:58160573-58160592 None:intergenic 40.0%
GCTGAAGACTATGGTAAACA+TGG + chr4_2:58160575-58160594 Msa0610450:CDS 40.0%
! AAGAGTTCAGGCAATTTTCC+AGG - chr4_2:58160733-58160752 None:intergenic 40.0%
GGAAAATTGCCTGAACTCTT+TGG + chr4_2:58160733-58160752 Msa0610450:three_prime_UTR 40.0%
ACATGCAACCAGATGCAAAA+TGG + chr4_2:58160759-58160778 Msa0610450:three_prime_UTR 40.0%
AGATTGTTACACACACTGCG+TGG + chr4_2:58159429-58159448 Msa0610450:CDS 45.0%
TGGAGAAGAAGAGGAAGAAG+AGG + chr4_2:58159496-58159515 Msa0610450:CDS 45.0%
GGAGAAGAAGAGGAAGAAGA+GGG + chr4_2:58159497-58159516 Msa0610450:CDS 45.0%
!! CGTAACGATGAACTGGTCTT+TGG - chr4_2:58159532-58159551 None:intergenic 45.0%
CGAGGTAGGATAAGGATTCA+GGG - chr4_2:58159557-58159576 None:intergenic 45.0%
TCGAGGTAGGATAAGGATTC+AGG - chr4_2:58159558-58159577 None:intergenic 45.0%
ATCCAGATCCCAAGAGTTGT+TGG + chr4_2:58159754-58159773 Msa0610450:CDS 45.0%
AAGAATGCTCCTGAAGGTCA+AGG + chr4_2:58159788-58159807 Msa0610450:CDS 45.0%
GGAATTGGGACGAGAAAACT+TGG - chr4_2:58159885-58159904 None:intergenic 45.0%
CCCAATTCCAGAATCAGAAC+TGG + chr4_2:58159896-58159915 Msa0610450:CDS 45.0%
TCATCAGCATTGTCTGTCTC+TGG - chr4_2:58159985-58160004 None:intergenic 45.0%
TGCTGATGACCTATGTAGCT+CGG + chr4_2:58159996-58160015 Msa0610450:intron 45.0%
! TCAAAGTGTCCGAGCTACAT+AGG - chr4_2:58160008-58160027 None:intergenic 45.0%
TCGTGTGTGTGTCAAGTGAA+AGG - chr4_2:58160405-58160424 None:intergenic 45.0%
ATGTCTACAGGAGGACGTAA+AGG + chr4_2:58160514-58160533 Msa0610450:intron 45.0%
TACAGGAGGACGTAAAGGAT+TGG + chr4_2:58160519-58160538 Msa0610450:intron 45.0%
GCATGTTTGCCAAAGAGTTC+AGG - chr4_2:58160745-58160764 None:intergenic 45.0%
AGATTGTTACACACACTGCG+TGG + chr4_2:58159429-58159448 Msa0610450:CDS 45.0%
TGGAGAAGAAGAGGAAGAAG+AGG + chr4_2:58159496-58159515 Msa0610450:CDS 45.0%
GGAGAAGAAGAGGAAGAAGA+GGG + chr4_2:58159497-58159516 Msa0610450:CDS 45.0%
!! CGTAACGATGAACTGGTCTT+TGG - chr4_2:58159532-58159551 None:intergenic 45.0%
CGAGGTAGGATAAGGATTCA+GGG - chr4_2:58159557-58159576 None:intergenic 45.0%
TCGAGGTAGGATAAGGATTC+AGG - chr4_2:58159558-58159577 None:intergenic 45.0%
ATCCAGATCCCAAGAGTTGT+TGG + chr4_2:58159754-58159773 Msa0610450:CDS 45.0%
AAGAATGCTCCTGAAGGTCA+AGG + chr4_2:58159788-58159807 Msa0610450:CDS 45.0%
GGAATTGGGACGAGAAAACT+TGG - chr4_2:58159885-58159904 None:intergenic 45.0%
CCCAATTCCAGAATCAGAAC+TGG + chr4_2:58159896-58159915 Msa0610450:CDS 45.0%
TCATCAGCATTGTCTGTCTC+TGG - chr4_2:58159985-58160004 None:intergenic 45.0%
TGCTGATGACCTATGTAGCT+CGG + chr4_2:58159996-58160015 Msa0610450:intron 45.0%
! TCAAAGTGTCCGAGCTACAT+AGG - chr4_2:58160008-58160027 None:intergenic 45.0%
TCGTGTGTGTGTCAAGTGAA+AGG - chr4_2:58160405-58160424 None:intergenic 45.0%
ATGTCTACAGGAGGACGTAA+AGG + chr4_2:58160514-58160533 Msa0610450:intron 45.0%
TACAGGAGGACGTAAAGGAT+TGG + chr4_2:58160519-58160538 Msa0610450:intron 45.0%
GCATGTTTGCCAAAGAGTTC+AGG - chr4_2:58160745-58160764 None:intergenic 45.0%
CCAACAGCAATGCCGATCAT+GGG - chr4_2:58159594-58159613 None:intergenic 50.0%
CTCCAACAACTCTTGGGATC+TGG - chr4_2:58159759-58159778 None:intergenic 50.0%
CCTACGCTCCAACAACTCTT+GGG - chr4_2:58159765-58159784 None:intergenic 50.0%
TCCTACGCTCCAACAACTCT+TGG - chr4_2:58159766-58159785 None:intergenic 50.0%
CTCACAGTACCTTGACCTTC+AGG - chr4_2:58159800-58159819 None:intergenic 50.0%
AATGCTCAGCCGTGAAGAGT+GGG + chr4_2:58159820-58159839 Msa0610450:CDS 50.0%
GAACCTTGTCCCACTCTTCA+CGG - chr4_2:58159832-58159851 None:intergenic 50.0%
GTTGCCAGAGCTGAAGACTA+TGG + chr4_2:58160566-58160585 Msa0610450:CDS 50.0%
CCAACAGCAATGCCGATCAT+GGG - chr4_2:58159594-58159613 None:intergenic 50.0%
CTCCAACAACTCTTGGGATC+TGG - chr4_2:58159759-58159778 None:intergenic 50.0%
CCTACGCTCCAACAACTCTT+GGG - chr4_2:58159765-58159784 None:intergenic 50.0%
TCCTACGCTCCAACAACTCT+TGG - chr4_2:58159766-58159785 None:intergenic 50.0%
CTCACAGTACCTTGACCTTC+AGG - chr4_2:58159800-58159819 None:intergenic 50.0%
AATGCTCAGCCGTGAAGAGT+GGG + chr4_2:58159820-58159839 Msa0610450:CDS 50.0%
GAACCTTGTCCCACTCTTCA+CGG - chr4_2:58159832-58159851 None:intergenic 50.0%
GTTGCCAGAGCTGAAGACTA+TGG + chr4_2:58160566-58160585 Msa0610450:CDS 50.0%
CAGGGACCGTAACGATGAAC+TGG - chr4_2:58159539-58159558 None:intergenic 55.0%
! TGCGGTGTCGAGGTAGGATA+AGG - chr4_2:58159565-58159584 None:intergenic 55.0%
ACCGCATCTCTGCCCATGAT+CGG + chr4_2:58159579-58159598 Msa0610450:CDS 55.0%
CCCATGATCGGCATTGCTGT+TGG + chr4_2:58159591-58159610 Msa0610450:CDS 55.0%
GCCAACAGCAATGCCGATCA+TGG - chr4_2:58159595-58159614 None:intergenic 55.0%
CCCAAGAGTTGTTGGAGCGT+AGG + chr4_2:58159762-58159781 Msa0610450:CDS 55.0%
GAATGCTCAGCCGTGAAGAG+TGG + chr4_2:58159819-58159838 Msa0610450:CDS 55.0%
GGGACAAGGTTCGAGAACTC+AGG + chr4_2:58159840-58159859 Msa0610450:CDS 55.0%
CAGGGACCGTAACGATGAAC+TGG - chr4_2:58159539-58159558 None:intergenic 55.0%
! TGCGGTGTCGAGGTAGGATA+AGG - chr4_2:58159565-58159584 None:intergenic 55.0%
ACCGCATCTCTGCCCATGAT+CGG + chr4_2:58159579-58159598 Msa0610450:CDS 55.0%
CCCATGATCGGCATTGCTGT+TGG + chr4_2:58159591-58159610 Msa0610450:CDS 55.0%
GCCAACAGCAATGCCGATCA+TGG - chr4_2:58159595-58159614 None:intergenic 55.0%
CCCAAGAGTTGTTGGAGCGT+AGG + chr4_2:58159762-58159781 Msa0610450:CDS 55.0%
GAATGCTCAGCCGTGAAGAG+TGG + chr4_2:58159819-58159838 Msa0610450:CDS 55.0%
GGGACAAGGTTCGAGAACTC+AGG + chr4_2:58159840-58159859 Msa0610450:CDS 55.0%
! CAGAGATGCGGTGTCGAGGT+AGG - chr4_2:58159571-58159590 None:intergenic 60.0%
GCCGATCATGGGCAGAGATG+CGG - chr4_2:58159583-58159602 None:intergenic 60.0%
CAGCCGTGAAGAGTGGGACA+AGG + chr4_2:58159826-58159845 Msa0610450:CDS 60.0%
! CAGAGATGCGGTGTCGAGGT+AGG - chr4_2:58159571-58159590 None:intergenic 60.0%
GCCGATCATGGGCAGAGATG+CGG - chr4_2:58159583-58159602 None:intergenic 60.0%
CAGCCGTGAAGAGTGGGACA+AGG + chr4_2:58159826-58159845 Msa0610450:CDS 60.0%
! TGGGCAGAGATGCGGTGTCG+AGG - chr4_2:58159575-58159594 None:intergenic 65.0%
! TGGGCAGAGATGCGGTGTCG+AGG - chr4_2:58159575-58159594 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58159344 58160964 58159344 ID=Msa0610450;Name=Msa0610450
chr4_2 mRNA 58159344 58160964 58159344 ID=Msa0610450-mRNA-1;Parent=Msa0610450;Name=Msa0610450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=67|1|1|1|1|1|3|357|168
chr4_2 exon 58159344 58159644 58159344 ID=Msa0610450-mRNA-1:exon:15234;Parent=Msa0610450-mRNA-1
chr4_2 exon 58159746 58159934 58159746 ID=Msa0610450-mRNA-1:exon:15235;Parent=Msa0610450-mRNA-1
chr4_2 exon 58160524 58160964 58160524 ID=Msa0610450-mRNA-1:exon:15236;Parent=Msa0610450-mRNA-1
chr4_2 five_prime_UTR 58159344 58159410 58159344 ID=Msa0610450-mRNA-1:five_prime_utr;Parent=Msa0610450-mRNA-1
chr4_2 CDS 58159411 58159644 58159411 ID=Msa0610450-mRNA-1:cds;Parent=Msa0610450-mRNA-1
chr4_2 CDS 58159746 58159934 58159746 ID=Msa0610450-mRNA-1:cds;Parent=Msa0610450-mRNA-1
chr4_2 CDS 58160524 58160607 58160524 ID=Msa0610450-mRNA-1:cds;Parent=Msa0610450-mRNA-1
chr4_2 three_prime_UTR 58160608 58160964 58160608 ID=Msa0610450-mRNA-1:three_prime_utr;Parent=Msa0610450-mRNA-1
Gene Sequence

>Msa0610450

ATGAACGTAATAAGACAAAGATTGTTACACACACTGCGTGGCGATGTCTTCCCATCTGAAGCATTGAAGAAAAAGGCATTGGAATTGGAGAAGAAGAGGAAGAAGAGGGCTTCTAAGACCAAAGACCAGTTCATCGTTACGGTCCCTGAATCCTTATCCTACCTCGACACCGCATCTCTGCCCATGATCGGCATTGCTGTTGGCATTGCAGTTTTCGCCAAACTTCTCATGATGTTAGATGAATCCAGATCCCAAGAGTTGTTGGAGCGTAGGATAAAGAATGCTCCTGAAGGTCAAGGTACTGTGAGAATGCTCAGCCGTGAAGAGTGGGACAAGGTTCGAGAACTCAGGCCTAGAACTCCGTATGAATCCAAGTTTTCTCGTCCCAATTCCAGAATCAGAACTGGAGAAAAATTGCGTTTGGAGGACGTAAAGGATTGGACAATTGATGTTTTCATGGATGGTGTTGCCAGAGCTGAAGACTATGGTAAACATGGTCCTAAGTAA
Protein sequence

>Msa0610450

MNVIRQRLLHTLRGDVFPSEALKKKALELEKKRKKRASKTKDQFIVTVPESLSYLDTASLPMIGIAVGIAVFAKLLMMLDESRSQELLERRIKNAPEGQGTVRMLSREEWDKVRELRPRTPYESKFSRPNSRIRTGEKLRLEDVKDWTIDVFMDGVARAEDYGKHGPK*