AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0651980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651980 MtrunA17_Chr4g0045361 95.238 420 17 1 1 420 1 417 0.0 820
Msa0651980 MtrunA17_Chr4g0062921 40.053 377 157 14 7 367 5 328 1.18e-66 218
Msa0651980 MtrunA17_Chr5g0402631 39.216 408 155 11 8 410 27 346 4.30e-61 201
Msa0651980 MtrunA17_Chr1g0198601 35.097 359 143 12 44 371 10 309 5.53e-46 162
Msa0651980 MtrunA17_Chr5g0422621 35.942 345 142 12 41 371 16 295 4.40e-44 157
Msa0651980 MtrunA17_Chr7g0255711 50.746 67 32 1 270 336 84 149 3.23e-15 75.9
Msa0651980 MtrunA17_Chr1g0175081 51.724 58 28 0 274 331 19 76 4.45e-15 70.9
Msa0651980 MtrunA17_Chr7g0243081 40.698 86 51 0 245 330 39 124 3.30e-14 73.6
Msa0651980 MtrunA17_Chr2g0288601 50.769 65 32 0 263 327 57 121 4.27e-14 68.9
Msa0651980 MtrunA17_Chr5g0417521 55.172 58 26 0 274 331 12 69 7.50e-14 72.4
Msa0651980 MtrunA17_Chr2g0304841 48.684 76 36 3 276 351 212 284 9.34e-14 73.2
Msa0651980 MtrunA17_Chr1g0190301 61.111 54 21 0 277 330 175 228 1.12e-13 72.0
Msa0651980 MtrunA17_Chr7g0261911 51.471 68 33 0 277 344 262 329 1.19e-13 72.4
Msa0651980 MtrunA17_Chr7g0274471 54.545 66 30 0 277 342 218 283 1.25e-13 72.4
Msa0651980 MtrunA17_Chr4g0031961 45.455 77 36 2 273 344 127 202 1.43e-13 71.2
Msa0651980 MtrunA17_Chr5g0400241 47.826 69 35 1 268 336 302 369 2.13e-13 72.0
Msa0651980 MtrunA17_Chr7g0254981 53.968 63 29 0 269 331 42 104 2.36e-13 70.1
Msa0651980 MtrunA17_Chr8g0366281 48.980 98 43 4 251 341 142 239 3.56e-13 70.5
Msa0651980 MtrunA17_Chr1g0162931 63.265 49 18 0 282 330 23 71 3.85e-13 69.7
Msa0651980 MtrunA17_Chr4g0067981 53.521 71 29 2 274 344 212 278 5.19e-13 70.9
Msa0651980 MtrunA17_Chr1g0149841 50.769 65 31 1 272 336 186 249 6.95e-13 70.1
Msa0651980 MtrunA17_Chr1g0210911 61.224 49 19 0 282 330 27 75 7.23e-13 69.3
Msa0651980 MtrunA17_Chr2g0291021 60.000 55 22 0 277 331 49 103 8.44e-13 68.9
Msa0651980 MtrunA17_Chr1g0175091 49.254 67 33 1 274 340 255 320 1.09e-12 69.3
Msa0651980 MtrunA17_Chr4g0054981 31.250 128 76 2 209 336 253 368 1.15e-12 69.7
Msa0651980 MtrunA17_Chr2g0294911 50.746 67 32 1 274 340 208 273 1.46e-12 69.3
Msa0651980 MtrunA17_Chr3g0137101 42.529 87 38 2 276 351 197 282 1.48e-12 69.3
Msa0651980 MtrunA17_Chr2g0321461 53.448 58 27 0 274 331 42 99 2.17e-12 68.2
Msa0651980 MtrunA17_Chr4g0040481 42.697 89 46 2 273 357 154 241 2.30e-12 68.2
Msa0651980 MtrunA17_Chr6g0461641 44.000 75 41 1 274 348 39 112 2.55e-12 68.2
Msa0651980 MtrunA17_Chr5g0410741 46.465 99 48 2 248 341 187 285 2.84e-12 67.8
Msa0651980 MtrunA17_Chr1g0161431 50.794 63 30 1 274 336 197 258 4.47e-12 67.8
Msa0651980 MtrunA17_Chr6g0479431 41.935 93 47 2 264 349 184 276 7.36e-12 66.6
Msa0651980 MtrunA17_Chr1g0149831 49.180 61 27 1 276 336 127 183 9.41e-12 64.7
Msa0651980 MtrunA17_Chr4g0074411 51.613 62 29 1 274 335 198 258 1.18e-11 66.6
Msa0651980 MtrunA17_Chr5g0409731 54.098 61 26 1 277 335 25 85 1.25e-11 65.1
Msa0651980 MtrunA17_Chr8g0356501 55.172 58 26 0 274 331 34 91 1.29e-11 65.5
Msa0651980 MtrunA17_Chr4g0042451 51.724 58 28 0 274 331 14 71 1.42e-11 65.5
Msa0651980 MtrunA17_Chr4g0009901 49.180 61 30 1 275 335 166 225 2.03e-11 64.3
Msa0651980 MtrunA17_Chr1g0197171 56.364 55 24 0 277 331 44 98 2.34e-11 64.3
Msa0651980 MtrunA17_Chr2g0324891 49.180 61 30 1 276 336 160 219 2.40e-11 65.1
Msa0651980 MtrunA17_Chr3g0095611 50.000 56 28 0 275 330 17 72 2.45e-11 63.2
Msa0651980 MtrunA17_Chr2g0310011 55.172 58 26 0 274 331 32 89 2.70e-11 64.7
Msa0651980 MtrunA17_Chr2g0320231 45.455 66 36 0 265 330 85 150 4.52e-11 64.3
Msa0651980 MtrunA17_Chr7g0258551 50.877 57 28 0 274 330 209 265 5.24e-11 63.9
Msa0651980 MtrunA17_Chr2g0290991 56.364 55 24 0 277 331 39 93 5.49e-11 63.5
Msa0651980 MtrunA17_Chr1g0154261 41.975 81 45 1 272 352 121 199 8.21e-11 62.8
Msa0651980 MtrunA17_Chr4g0074401 48.387 62 31 1 274 335 200 260 9.81e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0651980 AT2G03500.1 40.800 375 157 10 7 368 5 327 2.58e-65 215
Msa0651980 AT4G37180.1 36.216 370 160 10 9 377 12 306 2.75e-50 174
Msa0651980 AT4G37180.2 36.022 372 165 12 9 377 12 313 5.12e-48 168
Msa0651980 AT4G37180.3 35.737 319 135 8 9 327 12 260 1.62e-43 154
Msa0651980 AT1G49560.1 53.797 158 57 5 261 406 172 325 3.48e-38 141
Msa0651980 AT1G68670.1 69.892 93 20 1 274 358 212 304 1.27e-35 134
Msa0651980 AT1G68670.1 35.000 120 62 4 36 139 8 127 1.24e-11 66.2
Msa0651980 AT1G25550.1 54.622 119 47 2 274 385 206 324 5.02e-34 130
Msa0651980 AT1G25550.1 33.588 131 67 2 36 146 12 142 1.01e-14 75.5
Msa0651980 AT1G13300.1 70.000 90 22 2 275 359 179 268 7.61e-34 129
Msa0651980 AT3G25790.1 58.586 99 30 2 281 371 200 295 7.44e-31 122
Msa0651980 AT1G49560.2 66.667 81 21 2 251 327 164 242 6.13e-25 103
Msa0651980 AT1G25550.2 83.333 54 9 0 274 327 206 259 1.12e-24 103
Msa0651980 AT1G25550.2 33.588 131 67 2 36 146 12 142 2.07e-14 73.6
Msa0651980 AT3G25790.2 78.723 47 10 0 281 327 200 246 1.64e-19 88.2
Msa0651980 AT3G10760.1 50.704 71 34 1 266 336 94 163 2.59e-15 77.0
Msa0651980 AT5G05090.1 50.746 67 32 1 270 336 74 139 3.06e-15 75.9
Msa0651980 AT2G40970.1 50.746 67 32 1 270 336 98 163 3.97e-15 75.1
Msa0651980 AT2G38300.1 46.053 76 41 0 255 330 32 107 1.53e-14 74.7
Msa0651980 AT5G59570.2 50.000 64 31 1 273 336 137 199 1.81e-14 73.9
Msa0651980 AT5G59570.1 50.000 64 31 1 273 336 137 199 1.81e-14 73.9
Msa0651980 AT4G28610.1 56.061 66 28 1 277 342 225 289 6.61e-14 73.6
Msa0651980 AT3G04450.3 50.685 73 35 1 258 330 220 291 1.62e-13 71.6
Msa0651980 AT2G06020.1 35.652 115 72 1 232 346 41 153 2.06e-13 70.9
Msa0651980 AT2G20400.1 54.688 64 28 1 277 340 231 293 2.30e-13 71.6
Msa0651980 AT2G20400.2 54.688 64 28 1 277 340 231 293 2.40e-13 71.6
Msa0651980 AT3G16857.3 43.137 102 44 2 276 377 166 253 3.41e-13 72.0
Msa0651980 AT3G16857.2 43.137 102 44 2 276 377 236 323 3.46e-13 72.0
Msa0651980 AT3G46640.2 44.156 77 37 2 273 344 140 215 3.63e-13 70.5
Msa0651980 AT3G46640.1 44.156 77 37 2 273 344 140 215 3.63e-13 70.5
Msa0651980 AT5G58080.1 50.794 63 30 1 274 336 191 252 3.65e-13 71.6
Msa0651980 AT3G13040.3 58.929 56 23 0 276 331 240 295 3.96e-13 71.2
Msa0651980 AT3G13040.1 58.929 56 23 0 276 331 240 295 3.96e-13 71.2
Msa0651980 AT3G13040.2 58.929 56 23 0 276 331 240 295 3.96e-13 71.2
Msa0651980 AT3G04450.1 50.685 73 35 1 258 330 220 291 4.35e-13 71.2
Msa0651980 AT3G46640.3 44.156 77 37 2 273 344 140 215 4.44e-13 70.1
Msa0651980 AT3G16857.1 43.137 102 44 2 276 377 236 323 4.47e-13 71.6
Msa0651980 AT2G01760.3 54.839 62 27 1 275 336 133 193 5.48e-13 70.1
Msa0651980 AT3G04450.4 50.685 73 35 1 258 330 180 251 5.50e-13 70.5
Msa0651980 AT3G04450.2 50.685 73 35 1 258 330 180 251 5.50e-13 70.5
Msa0651980 AT2G01760.2 54.839 62 27 1 275 336 226 286 6.28e-13 70.5
Msa0651980 AT2G01760.1 54.839 62 27 1 275 336 198 258 6.78e-13 70.1
Msa0651980 AT2G01060.1 60.000 55 22 0 277 331 15 69 7.13e-13 69.3
Msa0651980 AT5G29000.4 56.140 57 25 0 274 330 228 284 1.33e-12 68.6
Msa0651980 AT5G18240.2 55.172 58 26 0 274 331 42 99 1.64e-12 68.9
Msa0651980 AT5G18240.3 55.172 58 26 0 274 331 42 99 1.64e-12 68.9
Msa0651980 AT5G18240.5 55.172 58 26 0 274 331 42 99 1.91e-12 68.9
Msa0651980 AT5G18240.1 55.172 58 26 0 274 331 42 99 1.94e-12 68.9
Msa0651980 AT5G18240.4 55.172 58 26 0 274 331 42 99 1.94e-12 68.9
Msa0651980 AT3G04030.1 53.448 58 27 0 274 331 42 99 2.30e-12 68.6
Msa0651980 AT3G04030.3 53.448 58 27 0 274 331 42 99 2.65e-12 68.6
Msa0651980 AT5G29000.1 59.259 54 22 0 277 330 188 241 3.66e-12 67.8
Msa0651980 AT5G29000.3 59.259 54 22 0 277 330 188 241 3.66e-12 67.8
Msa0651980 AT5G29000.2 59.259 54 22 0 277 330 231 284 3.70e-12 68.2
Msa0651980 AT5G06800.3 44.156 77 40 2 255 331 173 246 6.00e-12 66.2
Msa0651980 AT2G02060.2 50.000 62 31 0 269 330 21 82 6.43e-12 65.9
Msa0651980 AT2G02060.1 50.000 62 31 0 269 330 21 82 6.43e-12 65.9
Msa0651980 AT5G45580.1 48.529 68 35 0 277 344 7 74 6.61e-12 65.9
Msa0651980 AT1G14600.1 51.613 62 30 0 269 330 15 76 7.24e-12 65.9
Msa0651980 AT5G45580.2 47.826 69 36 0 277 345 23 91 7.35e-12 66.2
Msa0651980 AT2G25180.1 49.180 61 30 1 276 336 194 253 8.60e-12 67.4
Msa0651980 AT3G12730.1 56.364 55 24 0 277 331 23 77 1.04e-11 65.1
Msa0651980 AT1G67710.2 51.724 58 28 0 279 336 130 187 2.61e-11 65.5
Msa0651980 AT1G67710.3 51.724 58 28 0 279 336 121 178 2.65e-11 65.5
Msa0651980 AT1G67710.1 51.724 58 28 0 279 336 195 252 3.60e-11 65.5
Msa0651980 AT2G40260.1 41.772 79 44 1 266 344 71 147 3.63e-11 65.1
Msa0651980 AT5G06800.2 44.156 77 40 2 255 331 173 246 3.65e-11 64.7
Msa0651980 AT1G32240.2 44.318 88 48 1 265 351 200 287 3.97e-11 64.7
Msa0651980 AT5G06800.1 44.156 77 40 2 255 331 173 246 4.24e-11 64.7
Msa0651980 AT1G79430.2 55.172 58 26 0 274 331 31 88 4.45e-11 64.3
Msa0651980 AT3G24120.1 56.364 55 24 0 277 331 41 95 4.79e-11 63.9
Msa0651980 AT4G13640.1 56.364 55 24 0 277 331 37 91 4.98e-11 63.9
Msa0651980 AT1G32240.1 44.318 88 48 1 265 351 200 287 5.21e-11 64.3
Msa0651980 AT4G13640.2 56.364 55 24 0 277 331 37 91 5.30e-11 63.5
Msa0651980 AT3G24120.2 56.364 55 24 0 277 331 41 95 5.33e-11 63.5
Msa0651980 AT2G42660.1 55.102 49 22 0 282 330 55 103 8.41e-11 62.8

Find 137 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGTTCTTGGAGGTTTA+TGG 0.186325 4_3:-59584224 Msa0651980:CDS
GTGATGAGATTTATATAAAA+TGG 0.205392 4_3:+59586688 None:intergenic
CTCTTTCCTTTCTGCATTGT+AGG 0.213979 4_3:-59583475 Msa0651980:three_prime_UTR
GGTTCTTCCTTCTCTTCTTT+AGG 0.234160 4_3:+59585513 None:intergenic
TGACAGAATTTGCCCTTAAA+AGG 0.238192 4_3:+59583498 None:intergenic
TTCTTGTTGAGGAGTTAAAA+AGG 0.249808 4_3:+59586645 None:intergenic
AAAGAGAGCCTATTCCTTAC+AGG 0.270823 4_3:+59585492 None:intergenic
TGGACTAATAATATCTCTTC+TGG 0.271849 4_3:+59583740 None:intergenic
TTTCTGCATTGTAGGGTTGT+AGG 0.291103 4_3:-59583467 Msa0651980:three_prime_UTR
TTCAGCCGCAGCAGACTTCT+AGG 0.300158 4_3:-59585344 Msa0651980:CDS
GAGGAAATTAGCGATGGTTT+TGG 0.303370 4_3:+59586486 None:intergenic
TGAATTTAGGCCGTTAGATT+TGG 0.321583 4_3:+59586581 None:intergenic
ACAAATCTCCGGTGCAATTC+CGG 0.337882 4_3:+59585300 None:intergenic
ATTAGTCCAATGTTATGTTT+TGG 0.349670 4_3:-59583726 Msa0651980:three_prime_UTR
GGAATAAACTCTTCCAAAAC+TGG 0.355348 4_3:+59586094 None:intergenic
GATCAGTCTGTTGTAGTTCT+TGG 0.356587 4_3:-59584234 Msa0651980:CDS
TAGATGTGAATGTGAAATTA+AGG 0.363270 4_3:+59586804 None:intergenic
ATGGAGTTTGGAGAGTTTAT+CGG 0.366175 4_3:+59586435 None:intergenic
ATGAGTTCTCTAATTTGCTT+TGG 0.366259 4_3:+59584657 None:intergenic
AGAGTTTATCGGAGGTTGTT+TGG 0.367071 4_3:+59586446 None:intergenic
TTCTGCATTGTAGGGTTGTA+GGG 0.369021 4_3:-59583466 Msa0651980:three_prime_UTR
AGAGAGGAAATCATGGAGTT+TGG 0.369125 4_3:+59586423 None:intergenic
TACTACTACAAGATTGAAAA+AGG 0.370440 4_3:+59585713 None:intergenic
GATCGAGAAACAGTTCTAAC+AGG 0.377734 4_3:-59585422 Msa0651980:CDS
GCAGCAAAGATAAGAAGAAT+TGG 0.382485 4_3:-59586000 Msa0651980:CDS
AGAGAACTCATGCAAGTTGA+TGG 0.388920 4_3:-59584645 Msa0651980:CDS
GGATGAGTTCTGTTCAGTTA+TGG 0.403657 4_3:-59585979 Msa0651980:CDS
CGCATACTCGGCTGCACGGT+TGG 0.404437 4_3:+59585378 None:intergenic
AAGAGTCTGAGGGAAGGATT+TGG 0.407195 4_3:-59585453 Msa0651980:CDS
TGATGAGATTTATATAAAAT+GGG 0.411321 4_3:+59586689 None:intergenic
AGGAAGAACCTGTAAGGAAT+AGG 0.418786 4_3:-59585500 Msa0651980:CDS
AATACGAACCGTCGTTTAAG+AGG 0.421808 4_3:+59586338 None:intergenic
ACATGGAAAAGTTGGTGTAT+AGG 0.423229 4_3:-59584019 Msa0651980:exon
GGACTAATAATATCTCTTCT+GGG 0.425648 4_3:+59583741 None:intergenic
ATGAGAGCCAGACCACAAAA+TGG 0.426624 4_3:+59583543 None:intergenic
AGACGCCGTACTGCTACCTT+TGG 0.431495 4_3:+59584173 None:intergenic
ATTCAAAGACATGGAAAAGT+TGG 0.433103 4_3:-59584027 Msa0651980:CDS
AATAATGTAATAAGAAGAAG+TGG 0.434366 4_3:+59586779 None:intergenic
TTCATTTAGCTACAGGATCC+CGG 0.434371 4_3:-59584131 Msa0651980:CDS
TCTTTCCTTTCTGCATTGTA+GGG 0.437001 4_3:-59583474 Msa0651980:three_prime_UTR
TACATTCCACGATAAGAATC+AGG 0.439928 4_3:-59583819 Msa0651980:three_prime_UTR
ATCGGAGGTTGTTTGGATTG+TGG 0.443195 4_3:+59586453 None:intergenic
TCTTCACTTCATCATTAGTC+AGG 0.447623 4_3:+59584622 None:intergenic
TATGTGTAAATAATACTGTA+AGG 0.450749 4_3:+59583691 None:intergenic
GAAGAAGAAGATTCTTGTTG+AGG 0.451264 4_3:+59586634 None:intergenic
CATCTCTGAAATTTGAGAAG+TGG 0.455715 4_3:+59583664 None:intergenic
GAATACATAGAAGAGGCTAT+TGG 0.458107 4_3:-59583917 Msa0651980:three_prime_UTR
GTTAATGCGTTGCAGAAGCT+TGG 0.458197 4_3:-59585279 Msa0651980:CDS
GTAGGACTAGGAGAAGATAA+TGG 0.459925 4_3:+59583378 None:intergenic
CAGTCTGTTGTAGTTCTTGG+AGG 0.460264 4_3:-59584231 Msa0651980:CDS
TCCCTCAGACTCTTCACCTC+AGG 0.461715 4_3:+59585462 None:intergenic
CAGTTGGCGATGTCCCTCCC+CGG 0.465539 4_3:+59584112 None:intergenic
AGTCCACTTCATTTAGCTAC+AGG 0.467143 4_3:-59584138 Msa0651980:CDS
TCTTGTAGTAGTAATAGAAA+TGG 0.468798 4_3:-59585703 Msa0651980:CDS
AAAGGTAGCAGTACGGCGTC+TGG 0.473278 4_3:-59584171 Msa0651980:CDS
TGATCAATCTCTTTCTTCAA+AGG 0.473313 4_3:+59586073 None:intergenic
AAATCTAAACTGAGTTCTGC+AGG 0.491660 4_3:+59586526 None:intergenic
TGAGGGAAGGATTTGGTTCG+AGG 0.493121 4_3:-59585446 Msa0651980:CDS
GAAGTCTGCTGCGGCTGAAG+TGG 0.500174 4_3:+59585348 None:intergenic
AAGAGAAGGAAGAACCTGTA+AGG 0.506152 4_3:-59585506 Msa0651980:CDS
TCATTTAGCTACAGGATCCC+GGG 0.515125 4_3:-59584130 Msa0651980:CDS
AGAGTATCGGTTCTCATAGC+AGG 0.516459 4_3:-59583427 Msa0651980:three_prime_UTR
ATCAGCACCTAAAGAAGAGA+AGG 0.517481 4_3:-59585520 Msa0651980:CDS
TGATTCTTATCGTGGAATGT+AGG 0.518021 4_3:+59583821 None:intergenic
TGTAGTAGTAATAGAAATGG+AGG 0.522008 4_3:-59585700 Msa0651980:CDS
CAGAAGCTTGGAGGTTCTCA+AGG 0.522479 4_3:-59585267 Msa0651980:intron
TTTGCAGATAAAAGTATACA+TGG 0.523808 4_3:-59583974 Msa0651980:three_prime_UTR
CTAGGAAACAGAGGAGATGC+TGG 0.524226 4_3:-59585326 Msa0651980:CDS
CGAGTCGAGAGAGGAAATCA+TGG 0.525149 4_3:+59586416 None:intergenic
CTCCTGAGGTGAAGAGTCTG+AGG 0.527871 4_3:-59585464 Msa0651980:CDS
GTGATAGATAGAGTAGGACT+AGG 0.528949 4_3:+59583366 None:intergenic
GAGTCGTTGTATTGTTCTTG+AGG 0.536314 4_3:+59584198 None:intergenic
TAATGGATCCTCTACGGTTG+CGG 0.540015 4_3:+59583395 None:intergenic
ATACTCGGCTGCACGGTTGG+AGG 0.545035 4_3:+59585381 None:intergenic
AGTTGGCGATGTCCCTCCCC+GGG 0.547122 4_3:+59584113 None:intergenic
TGATCGGTACCACTTGGAGC+TGG 0.549273 4_3:+59584252 None:intergenic
GCTAAATGAAGTGGACTTTG+TGG 0.551266 4_3:+59584144 None:intergenic
AATGCGTTGCAGAAGCTTGG+AGG 0.554542 4_3:-59585276 Msa0651980:CDS
AGAGAGAGCAACGACGAATG+AGG 0.557649 4_3:+59586882 None:intergenic
CATCCACCAAAACATAACAT+TGG 0.559517 4_3:+59583720 None:intergenic
ACAGAGGAGATGCTGGTCAC+CGG 0.560535 4_3:-59585319 Msa0651980:CDS
GAACTCTCCGCGTATGAAGT+CGG 0.562568 4_3:+59584274 None:intergenic
AAATGACCTGATTCTTATCG+TGG 0.563659 4_3:+59583813 None:intergenic
ATTTATATAAAATGGGAATG+AGG 0.564588 4_3:+59586696 None:intergenic
GCTGGTCACCGGAATTGCAC+CGG 0.565693 4_3:-59585308 Msa0651980:CDS
TAGCTACAGGATCCCGGGGA+GGG 0.570717 4_3:-59584125 Msa0651980:CDS
ACAGACTGATCGGTACCACT+TGG 0.572661 4_3:+59584246 None:intergenic
GCAACGCATTAACAAATCTC+CGG 0.573053 4_3:+59585289 None:intergenic
AGTGGCGCAGCACGCATACT+CGG 0.578170 4_3:+59585366 None:intergenic
AGAAGATAATGGATCCTCTA+CGG 0.579715 4_3:+59583389 None:intergenic
GGAGGGACATCGCCAACTGA+AGG 0.584248 4_3:-59584108 Msa0651980:CDS
GTTTGATTTAGAAAGAGTAT+CGG 0.588174 4_3:-59583440 Msa0651980:three_prime_UTR
ATGTGTAAATAATACTGTAA+GGG 0.590884 4_3:+59583692 None:intergenic
TCCATGCTCCTCTTAAACGA+CGG 0.593486 4_3:-59586346 Msa0651980:intron
AATACATGAATACATAGAAG+AGG 0.594019 4_3:-59583924 Msa0651980:three_prime_UTR
ACAATTTGTGACTGCAGCTG+AGG 0.594577 4_3:-59585737 Msa0651980:CDS
GCAGGAACAACACAACCCAT+TGG 0.594618 4_3:+59586544 None:intergenic
GCCAACTGAAGGTGATAGCA+TGG 0.596672 4_3:-59584097 Msa0651980:CDS
ATGATAGAGGAAATTAGCGA+TGG 0.597212 4_3:+59586480 None:intergenic
TGACAGAAAAGGGAGGAAGA+AGG 0.597320 4_3:-59585760 Msa0651980:intron
AAAAGTCATATTCAAAGACA+TGG 0.597800 4_3:-59584036 Msa0651980:CDS
AAGAACTACAACAGACTGAT+CGG 0.598391 4_3:+59584236 None:intergenic
TCTTAATAAATTAGAAACAA+AGG 0.606924 4_3:-59585891 Msa0651980:intron
GATCCTGTAGCTAAATGAAG+TGG 0.608872 4_3:+59584135 None:intergenic
TCCATGCTATCACCTTCAGT+TGG 0.614177 4_3:+59584096 None:intergenic
GACTAATAATATCTCTTCTG+GGG 0.614864 4_3:+59583742 None:intergenic
TGAATGTGAAATTAAGGATG+CGG 0.615466 4_3:+59586810 None:intergenic
TACAACCCTACAATGCAGAA+AGG 0.616109 4_3:+59583469 None:intergenic
CATCAACGATCCAAATCTAA+CGG 0.616647 4_3:-59586591 Msa0651980:five_prime_UTR
GATAATGTCACTGTTGTCAC+AGG 0.616977 4_3:+59585540 None:intergenic
AGCACGCATACTCGGCTGCA+CGG 0.618042 4_3:+59585374 None:intergenic
GTTCATCTTCGAGTCGAGAG+AGG 0.619371 4_3:+59586407 None:intergenic
GGATTGTGGAAAGATGATAG+AGG 0.619765 4_3:+59586467 None:intergenic
CAATACAACGACTCTTCCAA+AGG 0.620750 4_3:-59584189 Msa0651980:CDS
TCCTGAGGTGAAGAGTCTGA+GGG 0.627907 4_3:-59585463 Msa0651980:CDS
GAGGGAAGGATTTGGTTCGA+GGG 0.630808 4_3:-59585445 Msa0651980:CDS
TTTATCTGCAAAATTCATGG+TGG 0.631088 4_3:+59583985 None:intergenic
GAGGTGAAGAGTCTGAGGGA+AGG 0.631423 4_3:-59585459 Msa0651980:CDS
TTGCAGATAAAAGTATACAT+GGG 0.633381 4_3:-59583973 Msa0651980:three_prime_UTR
GAGTTTGGAGAGTTTATCGG+AGG 0.638169 4_3:+59586438 None:intergenic
TTTAAGAGGAGCATGGAGAG+AGG 0.641505 4_3:+59586352 None:intergenic
CTCTTCCAAAGGTAGCAGTA+CGG 0.648767 4_3:-59584178 Msa0651980:CDS
TCACAAGTGATAGATAGAGT+AGG 0.649562 4_3:+59583360 None:intergenic
ATAATGTAATAAGAAGAAGT+GGG 0.650074 4_3:+59586780 None:intergenic
AGGGAAGGATTTGGTTCGAG+GGG 0.651342 4_3:-59585444 Msa0651980:CDS
CAGATAAAAGTATACATGGG+AGG 0.654402 4_3:-59583970 Msa0651980:three_prime_UTR
ACTTCATACCGCAACCGTAG+AGG 0.656491 4_3:-59583403 Msa0651980:three_prime_UTR
AGCAGACTTCTAGGAAACAG+AGG 0.657415 4_3:-59585335 Msa0651980:CDS
CATTTAGCTACAGGATCCCG+GGG 0.660202 4_3:-59584129 Msa0651980:CDS
ACCGTCGTTTAAGAGGAGCA+TGG 0.663203 4_3:+59586345 None:intergenic
AGAAATACCGACTTCATACG+CGG 0.668200 4_3:-59584281 Msa0651980:CDS
ACCACGACAGGAAAACACAA+AGG 0.668802 4_3:+59583607 None:intergenic
ATCGAGAAACAGTTCTAACA+GGG 0.670598 4_3:-59585421 Msa0651980:CDS
TGTTTCCTAGAAGTCTGCTG+CGG 0.688682 4_3:+59585339 None:intergenic
TTAGCTACAGGATCCCGGGG+AGG 0.690072 4_3:-59584126 Msa0651980:CDS
CGGAGAGTTCCAGCTCCAAG+TGG 0.698331 4_3:-59584261 Msa0651980:CDS
TTGGTGATGACAGAAAAGGG+AGG 0.712344 4_3:-59585767 Msa0651980:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATGAGATTTATATAAAAT+GGG + chr4_3:59583556-59583575 None:intergenic 15.0%
!! TCTTAATAAATTAGAAACAA+AGG - chr4_3:59584351-59584370 Msa0651980:intron 15.0%
!!! ATTTTTTCTTGCTAATTTTT+AGG + chr4_3:59584420-59584439 None:intergenic 15.0%
!! AATCTATAATATTTCCAAAT+TGG + chr4_3:59585120-59585139 None:intergenic 15.0%
!! ATAATGTAATAAGAAGAAGT+GGG + chr4_3:59583465-59583484 None:intergenic 20.0%
!! AATAATGTAATAAGAAGAAG+TGG + chr4_3:59583466-59583485 None:intergenic 20.0%
!! ATTTATATAAAATGGGAATG+AGG + chr4_3:59583549-59583568 None:intergenic 20.0%
!! GTGATGAGATTTATATAAAA+TGG + chr4_3:59583557-59583576 None:intergenic 20.0%
!!! TCTTTTTGTTTGATGAATTT+AGG + chr4_3:59583677-59583696 None:intergenic 20.0%
!! TTGAATAAAGTTATCAACTT+TGG - chr4_3:59584005-59584024 Msa0651980:exon 20.0%
!! ATTATAGATTAGTGAATTGA+TGG - chr4_3:59585130-59585149 Msa0651980:intron 20.0%
!! TAATAACATTACATCTTGTT+TGG - chr4_3:59585522-59585541 Msa0651980:CDS 20.0%
!! ATGTGTAAATAATACTGTAA+GGG + chr4_3:59586553-59586572 None:intergenic 20.0%
!! TATGTGTAAATAATACTGTA+AGG + chr4_3:59586554-59586573 None:intergenic 20.0%
!! TTGTAAGCTGTAAAAAAAAA+AGG - chr4_3:59586721-59586740 Msa0651980:five_prime_UTR 20.0%
!!! TAAAAAAAAAAGGCCTTTTA+AGG - chr4_3:59586731-59586750 Msa0651980:five_prime_UTR 20.0%
!!! AAAAAAAAAAGGCCTTTTAA+GGG - chr4_3:59586732-59586751 Msa0651980:five_prime_UTR 20.0%
! TAGATGTGAATGTGAAATTA+AGG + chr4_3:59583441-59583460 None:intergenic 25.0%
! ATCAAACAAAAAGATCCAAT+GGG - chr4_3:59583683-59583702 Msa0651980:three_prime_UTR 25.0%
!! TGATGATGATGATATTGAAA+GGG + chr4_3:59583930-59583949 None:intergenic 25.0%
!! ATGATGATGATGATATTGAA+AGG + chr4_3:59583931-59583950 None:intergenic 25.0%
!! ACCCTTTTGAATTTTTTATG+TGG - chr4_3:59583979-59583998 Msa0651980:three_prime_UTR 25.0%
! CCCACATAAAAAATTCAAAA+GGG + chr4_3:59583983-59584002 None:intergenic 25.0%
!! CCCTTTTGAATTTTTTATGT+GGG - chr4_3:59583980-59583999 Msa0651980:three_prime_UTR 25.0%
! ACCCACATAAAAAATTCAAA+AGG + chr4_3:59583984-59584003 None:intergenic 25.0%
!!! TTTGGAGTTTTTGATTCAAT+GGG - chr4_3:59584023-59584042 Msa0651980:CDS 25.0%
! TACTACTACAAGATTGAAAA+AGG + chr4_3:59584532-59584551 None:intergenic 25.0%
! TCTTGTAGTAGTAATAGAAA+TGG - chr4_3:59584539-59584558 Msa0651980:intron 25.0%
!!! AAACTTTACTGTTTTTGTGA+TGG - chr4_3:59585008-59585027 Msa0651980:intron 25.0%
!!! ATGCTAGTCTTAAAAGTTTA+AGG - chr4_3:59585168-59585187 Msa0651980:intron 25.0%
! TGTTTAAATTTAGATCAGCA+AGG - chr4_3:59585272-59585291 Msa0651980:CDS 25.0%
! ATATAGACAAGTCAATTTGT+AGG - chr4_3:59585314-59585333 Msa0651980:CDS 25.0%
! GTTAAGACCAAAAAACATAT+AGG - chr4_3:59585441-59585460 Msa0651980:CDS 25.0%
!! CAATTTTGCATACATAGTTT+TGG - chr4_3:59585492-59585511 Msa0651980:CDS 25.0%
! CTTTATTAACCTTAGTTACT+AGG - chr4_3:59585699-59585718 Msa0651980:CDS 25.0%
! TTGAGTTATCAAGTTTGAAT+TGG - chr4_3:59585906-59585925 Msa0651980:CDS 25.0%
! AAAAGTCATATTCAAAGACA+TGG - chr4_3:59586206-59586225 Msa0651980:intron 25.0%
!! ACTTTTATCTGCAAAATTCA+TGG + chr4_3:59586263-59586282 None:intergenic 25.0%
!! TTTGCAGATAAAAGTATACA+TGG - chr4_3:59586268-59586287 Msa0651980:intron 25.0%
!! TTGCAGATAAAAGTATACAT+GGG - chr4_3:59586269-59586288 Msa0651980:intron 25.0%
! AATACATGAATACATAGAAG+AGG - chr4_3:59586318-59586337 Msa0651980:intron 25.0%
!! ATTAGTCCAATGTTATGTTT+TGG - chr4_3:59586516-59586535 Msa0651980:CDS 25.0%
!!! TTTATGTTATTGCCATTTTG+TGG - chr4_3:59586687-59586706 Msa0651980:five_prime_UTR 25.0%
!! GTTTGATTTAGAAAGAGTAT+CGG - chr4_3:59586802-59586821 Msa0651980:five_prime_UTR 25.0%
TGAATGTGAAATTAAGGATG+CGG + chr4_3:59583435-59583454 None:intergenic 30.0%
TCTTGTTGAGGAGTTAAAAA+GGG + chr4_3:59583599-59583618 None:intergenic 30.0%
TTCTTGTTGAGGAGTTAAAA+AGG + chr4_3:59583600-59583619 None:intergenic 30.0%
CATCAAACAAAAAGATCCAA+TGG - chr4_3:59583682-59583701 Msa0651980:three_prime_UTR 30.0%
!!! CTTTGGAGTTTTTGATTCAA+TGG - chr4_3:59584022-59584041 Msa0651980:CDS 30.0%
!! TTGGAGTTTTTGATTCAATG+GGG - chr4_3:59584024-59584043 Msa0651980:CDS 30.0%
! TTTTGTGTATGCAGCAATTT+TGG - chr4_3:59584081-59584100 Msa0651980:CDS 30.0%
! TAAAAATTCTGTGCCAGTTT+TGG - chr4_3:59584135-59584154 Msa0651980:CDS 30.0%
TGATCAATCTCTTTCTTCAA+AGG + chr4_3:59584172-59584191 None:intergenic 30.0%
TTGCAACAGTAATCTTAATG+AGG - chr4_3:59584439-59584458 Msa0651980:intron 30.0%
TGCAACAGTAATCTTAATGA+GGG - chr4_3:59584440-59584459 Msa0651980:intron 30.0%
!!! ATGAGGGTTTTGTTTTTGTT+TGG - chr4_3:59584456-59584475 Msa0651980:intron 30.0%
TGTAGTAGTAATAGAAATGG+AGG - chr4_3:59584542-59584561 Msa0651980:intron 30.0%
!! ACACTCAATTTTGACCAATT+TGG - chr4_3:59585103-59585122 Msa0651980:intron 30.0%
TGATAATCCATAGTTTGGTT+GGG + chr4_3:59585394-59585413 None:intergenic 30.0%
!!! TGATAGGCCTATATGTTTTT+TGG + chr4_3:59585451-59585470 None:intergenic 30.0%
AATTGCAAAGGCAAATCATT+TGG + chr4_3:59585477-59585496 None:intergenic 30.0%
CTATGTATGCAAAATTGCAA+AGG + chr4_3:59585489-59585508 None:intergenic 30.0%
!! ATGAGTTCTCTAATTTGCTT+TGG + chr4_3:59585588-59585607 None:intergenic 30.0%
ACCAAAAACTTACTTGCAAA+TGG + chr4_3:59585647-59585666 None:intergenic 30.0%
TAATAAAGCATGCAGTTAAG+AGG + chr4_3:59585687-59585706 None:intergenic 30.0%
TCATATTGTATGTGCATTCA+TGG - chr4_3:59585848-59585867 Msa0651980:intron 30.0%
AAAAATCTGAGAGTTATAGC+TGG - chr4_3:59586183-59586202 Msa0651980:intron 30.0%
ATTCAAAGACATGGAAAAGT+TGG - chr4_3:59586215-59586234 Msa0651980:intron 30.0%
TTTATCTGCAAAATTCATGG+TGG + chr4_3:59586260-59586279 None:intergenic 30.0%
!!! AGGCTATTGGAATTTTTTGA+AGG - chr4_3:59586338-59586357 Msa0651980:intron 30.0%
GACTAATAATATCTCTTCTG+GGG + chr4_3:59586503-59586522 None:intergenic 30.0%
GGACTAATAATATCTCTTCT+GGG + chr4_3:59586504-59586523 None:intergenic 30.0%
TGGACTAATAATATCTCTTC+TGG + chr4_3:59586505-59586524 None:intergenic 30.0%
CTTGTTGAGGAGTTAAAAAG+GGG + chr4_3:59583598-59583617 None:intergenic 35.0%
GAAGAAGAAGATTCTTGTTG+AGG + chr4_3:59583611-59583630 None:intergenic 35.0%
CATCAACGATCCAAATCTAA+CGG - chr4_3:59583651-59583670 Msa0651980:three_prime_UTR 35.0%
TGAATTTAGGCCGTTAGATT+TGG + chr4_3:59583664-59583683 None:intergenic 35.0%
AAATCTAAACTGAGTTCTGC+AGG + chr4_3:59583719-59583738 None:intergenic 35.0%
! AGGAAATTAGCGATGGTTTT+GGG + chr4_3:59583758-59583777 None:intergenic 35.0%
ATGATAGAGGAAATTAGCGA+TGG + chr4_3:59583765-59583784 None:intergenic 35.0%
ATGGAGTTTGGAGAGTTTAT+CGG + chr4_3:59583810-59583829 None:intergenic 35.0%
!! TTTTGATTCAATGGGGCTAA+TGG - chr4_3:59584031-59584050 Msa0651980:CDS 35.0%
TTTGATTCAATGGGGCTAAT+GGG - chr4_3:59584032-59584051 Msa0651980:CDS 35.0%
!!! TGCAGCAATTTTGGTTTTGA+AGG - chr4_3:59584090-59584109 Msa0651980:CDS 35.0%
!!! AGCAATTTTGGTTTTGAAGG+AGG - chr4_3:59584093-59584112 Msa0651980:CDS 35.0%
!!! TTTGGTTTTGAAGGAGGAAT+TGG - chr4_3:59584099-59584118 Msa0651980:CDS 35.0%
GGAATAAACTCTTCCAAAAC+TGG + chr4_3:59584151-59584170 None:intergenic 35.0%
GCAGCAAAGATAAGAAGAAT+TGG - chr4_3:59584242-59584261 Msa0651980:CDS 35.0%
!! TTGTTTGGTGATGACAGAAA+AGG - chr4_3:59584471-59584490 Msa0651980:intron 35.0%
!! TGTTTGGTGATGACAGAAAA+GGG - chr4_3:59584472-59584491 Msa0651980:intron 35.0%
! ATCGAGAAACAGTTCTAACA+GGG - chr4_3:59584821-59584840 Msa0651980:intron 35.0%
TCTTATGTAGTTGTGTTGTG+AGG - chr4_3:59585073-59585092 Msa0651980:intron 35.0%
! TTTAGTTCACCAGTGAAGTT+AGG - chr4_3:59585246-59585265 Msa0651980:intron 35.0%
TTAGTTCACCAGTGAAGTTA+GGG - chr4_3:59585247-59585266 Msa0651980:intron 35.0%
TAAACAATCCCTAACTTCAC+TGG + chr4_3:59585258-59585277 None:intergenic 35.0%
TCTATCCCGAATTCAAATCT+TGG - chr4_3:59585337-59585356 Msa0651980:CDS 35.0%
AATCTGATCAAGGATTGTTC+CGG + chr4_3:59585363-59585382 None:intergenic 35.0%
GGAGGTTATTAATCTGATCA+AGG + chr4_3:59585373-59585392 None:intergenic 35.0%
CTGATAATCCATAGTTTGGT+TGG + chr4_3:59585395-59585414 None:intergenic 35.0%
!!! GTTTTTTGGTCTTAACCTTC+CGG + chr4_3:59585437-59585456 None:intergenic 35.0%
GCAAATCATTTGGACTTGAT+AGG + chr4_3:59585467-59585486 None:intergenic 35.0%
TCTTCACTTCATCATTAGTC+AGG + chr4_3:59585623-59585642 None:intergenic 35.0%
! GCCATTTGCAAGTAAGTTTT+TGG - chr4_3:59585643-59585662 Msa0651980:intron 35.0%
CATCAAACACCTAGTAACTA+AGG + chr4_3:59585711-59585730 None:intergenic 35.0%
AGTTACTAGGTGTTTGATGA+TGG - chr4_3:59585712-59585731 Msa0651980:CDS 35.0%
! AGGCATTTAGGATTGAGATT+TGG - chr4_3:59585883-59585902 Msa0651980:intron 35.0%
AAGAACTACAACAGACTGAT+CGG + chr4_3:59586009-59586028 None:intergenic 35.0%
TTGTAGTTCTTGGAGGTTTA+TGG - chr4_3:59586018-59586037 Msa0651980:CDS 35.0%
!! ACATGGAAAAGTTGGTGTAT+AGG - chr4_3:59586223-59586242 Msa0651980:intron 35.0%
! CAGATAAAAGTATACATGGG+AGG - chr4_3:59586272-59586291 Msa0651980:intron 35.0%
GAATACATAGAAGAGGCTAT+TGG - chr4_3:59586325-59586344 Msa0651980:intron 35.0%
TGATTCTTATCGTGGAATGT+AGG + chr4_3:59586424-59586443 None:intergenic 35.0%
TACATTCCACGATAAGAATC+AGG - chr4_3:59586423-59586442 Msa0651980:CDS 35.0%
AAATGACCTGATTCTTATCG+TGG + chr4_3:59586432-59586451 None:intergenic 35.0%
!! AGTCCAATGTTATGTTTTGG+TGG - chr4_3:59586519-59586538 Msa0651980:CDS 35.0%
CATCCACCAAAACATAACAT+TGG + chr4_3:59586525-59586544 None:intergenic 35.0%
!! CATCTCTGAAATTTGAGAAG+TGG + chr4_3:59586581-59586600 None:intergenic 35.0%
! TTTTCTCAAAGACACTGAAC+AGG - chr4_3:59586659-59586678 Msa0651980:five_prime_UTR 35.0%
TGACAGAATTTGCCCTTAAA+AGG + chr4_3:59586747-59586766 None:intergenic 35.0%
TCTTTCCTTTCTGCATTGTA+GGG - chr4_3:59586768-59586787 Msa0651980:five_prime_UTR 35.0%
!! AGAAGATAATGGATCCTCTA+CGG + chr4_3:59586856-59586875 None:intergenic 35.0%
TCACAAGTGATAGATAGAGT+AGG + chr4_3:59586885-59586904 None:intergenic 35.0%
!!! ATGGTTTTGGGAACGAAAGT+TGG + chr4_3:59583746-59583765 None:intergenic 40.0%
! GAGGAAATTAGCGATGGTTT+TGG + chr4_3:59583759-59583778 None:intergenic 40.0%
GGATTGTGGAAAGATGATAG+AGG + chr4_3:59583778-59583797 None:intergenic 40.0%
AGAGTTTATCGGAGGTTGTT+TGG + chr4_3:59583799-59583818 None:intergenic 40.0%
AGAGAGGAAATCATGGAGTT+TGG + chr4_3:59583822-59583841 None:intergenic 40.0%
AATACGAACCGTCGTTTAAG+AGG + chr4_3:59583907-59583926 None:intergenic 40.0%
GGATGAGTTCTGTTCAGTTA+TGG - chr4_3:59584263-59584282 Msa0651980:CDS 40.0%
GATAATGTCACTGTTGTCAC+AGG + chr4_3:59584705-59584724 None:intergenic 40.0%
ATCAGCACCTAAAGAAGAGA+AGG - chr4_3:59584722-59584741 Msa0651980:intron 40.0%
GGTTCTTCCTTCTCTTCTTT+AGG + chr4_3:59584732-59584751 None:intergenic 40.0%
AAGAGAAGGAAGAACCTGTA+AGG - chr4_3:59584736-59584755 Msa0651980:intron 40.0%
AGGAAGAACCTGTAAGGAAT+AGG - chr4_3:59584742-59584761 Msa0651980:intron 40.0%
AAAGAGAGCCTATTCCTTAC+AGG + chr4_3:59584753-59584772 None:intergenic 40.0%
! GATCGAGAAACAGTTCTAAC+AGG - chr4_3:59584820-59584839 Msa0651980:intron 40.0%
GCAACGCATTAACAAATCTC+CGG + chr4_3:59584956-59584975 None:intergenic 40.0%
! TAGTGAATTGATGGATGTGC+TGG - chr4_3:59585139-59585158 Msa0651980:intron 40.0%
AGATCAGCAAGGAGACAAAT+AGG - chr4_3:59585283-59585302 Msa0651980:CDS 40.0%
TTCCGGCCAAGATTTGAATT+CGG + chr4_3:59585346-59585365 None:intergenic 40.0%
ATAACCTCCCAACCAAACTA+TGG - chr4_3:59585384-59585403 Msa0651980:CDS 40.0%
TAATCCATAGTTTGGTTGGG+AGG + chr4_3:59585391-59585410 None:intergenic 40.0%
GGGACTGATAATCCATAGTT+TGG + chr4_3:59585399-59585418 None:intergenic 40.0%
! AGAGAACTCATGCAAGTTGA+TGG - chr4_3:59585597-59585616 Msa0651980:intron 40.0%
ATTCATGGTGTCTGGTTATG+AGG - chr4_3:59585863-59585882 Msa0651980:intron 40.0%
! TGTCTGGTTATGAGGCATTT+AGG - chr4_3:59585871-59585890 Msa0651980:intron 40.0%
!! AGAAATACCGACTTCATACG+CGG - chr4_3:59585961-59585980 Msa0651980:CDS 40.0%
GATCAGTCTGTTGTAGTTCT+TGG - chr4_3:59586008-59586027 Msa0651980:CDS 40.0%
GAGTCGTTGTATTGTTCTTG+AGG + chr4_3:59586047-59586066 None:intergenic 40.0%
CAATACAACGACTCTTCCAA+AGG - chr4_3:59586053-59586072 Msa0651980:CDS 40.0%
! GCTAAATGAAGTGGACTTTG+TGG + chr4_3:59586101-59586120 None:intergenic 40.0%
AGTCCACTTCATTTAGCTAC+AGG - chr4_3:59586104-59586123 Msa0651980:CDS 40.0%
GATCCTGTAGCTAAATGAAG+TGG + chr4_3:59586110-59586129 None:intergenic 40.0%
TTCATTTAGCTACAGGATCC+CGG - chr4_3:59586111-59586130 Msa0651980:CDS 40.0%
! CTTTGAGAAAAAACCACGAC+AGG + chr4_3:59586650-59586669 None:intergenic 40.0%
!!! GTTATTGCCATTTTGTGGTC+TGG - chr4_3:59586692-59586711 Msa0651980:five_prime_UTR 40.0%
CTCTTTCCTTTCTGCATTGT+AGG - chr4_3:59586767-59586786 Msa0651980:five_prime_UTR 40.0%
TACAACCCTACAATGCAGAA+AGG + chr4_3:59586776-59586795 None:intergenic 40.0%
TTTCTGCATTGTAGGGTTGT+AGG - chr4_3:59586775-59586794 Msa0651980:five_prime_UTR 40.0%
TTCTGCATTGTAGGGTTGTA+GGG - chr4_3:59586776-59586795 Msa0651980:five_prime_UTR 40.0%
GTAGGACTAGGAGAAGATAA+TGG + chr4_3:59586867-59586886 None:intergenic 40.0%
GTGATAGATAGAGTAGGACT+AGG + chr4_3:59586879-59586898 None:intergenic 40.0%
ATCGGAGGTTGTTTGGATTG+TGG + chr4_3:59583792-59583811 None:intergenic 45.0%
GAGTTTGGAGAGTTTATCGG+AGG + chr4_3:59583807-59583826 None:intergenic 45.0%
! TTTAAGAGGAGCATGGAGAG+AGG + chr4_3:59583893-59583912 None:intergenic 45.0%
TCCATGCTCCTCTTAAACGA+CGG - chr4_3:59583896-59583915 Msa0651980:three_prime_UTR 45.0%
!! TTGGTGATGACAGAAAAGGG+AGG - chr4_3:59584475-59584494 Msa0651980:intron 45.0%
TGACAGAAAAGGGAGGAAGA+AGG - chr4_3:59584482-59584501 Msa0651980:intron 45.0%
ACAATTTGTGACTGCAGCTG+AGG - chr4_3:59584505-59584524 Msa0651980:intron 45.0%
AAGAGTCTGAGGGAAGGATT+TGG - chr4_3:59584789-59584808 Msa0651980:intron 45.0%
TGTTTCCTAGAAGTCTGCTG+CGG + chr4_3:59584906-59584925 None:intergenic 45.0%
! AGCAGACTTCTAGGAAACAG+AGG - chr4_3:59584907-59584926 Msa0651980:intron 45.0%
ACAAATCTCCGGTGCAATTC+CGG + chr4_3:59584945-59584964 None:intergenic 45.0%
GTTAATGCGTTGCAGAAGCT+TGG - chr4_3:59584963-59584982 Msa0651980:intron 45.0%
TCCCGAATTCAAATCTTGGC+CGG - chr4_3:59585341-59585360 Msa0651980:CDS 45.0%
TCCGGCCAAGATTTGAATTC+GGG + chr4_3:59585345-59585364 None:intergenic 45.0%
TCTTAACCTTCCGGTTCCTA+GGG + chr4_3:59585428-59585447 None:intergenic 45.0%
GTATGTGCATTCATGGTGTC+TGG - chr4_3:59585855-59585874 Msa0651980:intron 45.0%
CAGTCTGTTGTAGTTCTTGG+AGG - chr4_3:59586011-59586030 Msa0651980:CDS 45.0%
CTCTTCCAAAGGTAGCAGTA+CGG - chr4_3:59586064-59586083 Msa0651980:CDS 45.0%
TCATTTAGCTACAGGATCCC+GGG - chr4_3:59586112-59586131 Msa0651980:CDS 45.0%
TCCATGCTATCACCTTCAGT+TGG + chr4_3:59586149-59586168 None:intergenic 45.0%
! ACCTTTGTGTTTTCCTGTCG+TGG - chr4_3:59586634-59586653 Msa0651980:five_prime_UTR 45.0%
ACCACGACAGGAAAACACAA+AGG + chr4_3:59586638-59586657 None:intergenic 45.0%
ATGAGAGCCAGACCACAAAA+TGG + chr4_3:59586702-59586721 None:intergenic 45.0%
!! AGAGTATCGGTTCTCATAGC+AGG - chr4_3:59586815-59586834 Msa0651980:five_prime_UTR 45.0%
! TAATGGATCCTCTACGGTTG+CGG + chr4_3:59586850-59586869 None:intergenic 45.0%
AGAGAGAGCAACGACGAATG+AGG + chr4_3:59583363-59583382 None:intergenic 50.0%
GCAGGAACAACACAACCCAT+TGG + chr4_3:59583701-59583720 None:intergenic 50.0%
CGAGTCGAGAGAGGAAATCA+TGG + chr4_3:59583829-59583848 None:intergenic 50.0%
GTTCATCTTCGAGTCGAGAG+AGG + chr4_3:59583838-59583857 None:intergenic 50.0%
ACCGTCGTTTAAGAGGAGCA+TGG + chr4_3:59583900-59583919 None:intergenic 50.0%
! GCTCTCTTTTCTGACTCCTG+AGG - chr4_3:59584764-59584783 Msa0651980:intron 50.0%
TCCTGAGGTGAAGAGTCTGA+GGG - chr4_3:59584779-59584798 Msa0651980:intron 50.0%
! TGAGGGAAGGATTTGGTTCG+AGG - chr4_3:59584796-59584815 Msa0651980:intron 50.0%
! GAGGGAAGGATTTGGTTCGA+GGG - chr4_3:59584797-59584816 Msa0651980:intron 50.0%
! AGGGAAGGATTTGGTTCGAG+GGG - chr4_3:59584798-59584817 Msa0651980:intron 50.0%
CTAGGAAACAGAGGAGATGC+TGG - chr4_3:59584916-59584935 Msa0651980:intron 50.0%
AATGCGTTGCAGAAGCTTGG+AGG - chr4_3:59584966-59584985 Msa0651980:intron 50.0%
CAGAAGCTTGGAGGTTCTCA+AGG - chr4_3:59584975-59584994 Msa0651980:intron 50.0%
TATCAGTCCCTCCTTTCCCT+AGG - chr4_3:59585409-59585428 Msa0651980:CDS 50.0%
GTCTTAACCTTCCGGTTCCT+AGG + chr4_3:59585429-59585448 None:intergenic 50.0%
GAACTCTCCGCGTATGAAGT+CGG + chr4_3:59585971-59585990 None:intergenic 50.0%
ACAGACTGATCGGTACCACT+TGG + chr4_3:59585999-59586018 None:intergenic 50.0%
CATTTAGCTACAGGATCCCG+GGG - chr4_3:59586113-59586132 Msa0651980:CDS 50.0%
GCCAACTGAAGGTGATAGCA+TGG - chr4_3:59586145-59586164 Msa0651980:CDS 50.0%
ACTTCATACCGCAACCGTAG+AGG - chr4_3:59586839-59586858 Msa0651980:five_prime_UTR 50.0%
CTCCTGAGGTGAAGAGTCTG+AGG - chr4_3:59584778-59584797 Msa0651980:intron 55.0%
TCCCTCAGACTCTTCACCTC+AGG + chr4_3:59584783-59584802 None:intergenic 55.0%
GAGGTGAAGAGTCTGAGGGA+AGG - chr4_3:59584783-59584802 Msa0651980:intron 55.0%
TTCAGCCGCAGCAGACTTCT+AGG - chr4_3:59584898-59584917 Msa0651980:intron 55.0%
ACAGAGGAGATGCTGGTCAC+CGG - chr4_3:59584923-59584942 Msa0651980:intron 55.0%
TCCCTCCTTTCCCTAGGAAC+CGG - chr4_3:59585415-59585434 Msa0651980:CDS 55.0%
TCCGGTTCCTAGGGAAAGGA+GGG + chr4_3:59585419-59585438 None:intergenic 55.0%
TTCCGGTTCCTAGGGAAAGG+AGG + chr4_3:59585420-59585439 None:intergenic 55.0%
TCCTTTCCCTAGGAACCGGA+AGG - chr4_3:59585419-59585438 Msa0651980:CDS 55.0%
ACCTTCCGGTTCCTAGGGAA+AGG + chr4_3:59585423-59585442 None:intergenic 55.0%
TGATCGGTACCACTTGGAGC+TGG + chr4_3:59585993-59586012 None:intergenic 55.0%
AGACGCCGTACTGCTACCTT+TGG + chr4_3:59586072-59586091 None:intergenic 55.0%
AAAGGTAGCAGTACGGCGTC+TGG - chr4_3:59586071-59586090 Msa0651980:CDS 55.0%
ATACTCGGCTGCACGGTTGG+AGG + chr4_3:59584864-59584883 None:intergenic 60.0%
AGCACGCATACTCGGCTGCA+CGG + chr4_3:59584871-59584890 None:intergenic 60.0%
AGTGGCGCAGCACGCATACT+CGG + chr4_3:59584879-59584898 None:intergenic 60.0%
GAAGTCTGCTGCGGCTGAAG+TGG + chr4_3:59584897-59584916 None:intergenic 60.0%
GCTGGTCACCGGAATTGCAC+CGG - chr4_3:59584934-59584953 Msa0651980:intron 60.0%
CGGAGAGTTCCAGCTCCAAG+TGG - chr4_3:59585981-59586000 Msa0651980:CDS 60.0%
TTAGCTACAGGATCCCGGGG+AGG - chr4_3:59586116-59586135 Msa0651980:CDS 60.0%
TAGCTACAGGATCCCGGGGA+GGG - chr4_3:59586117-59586136 Msa0651980:CDS 60.0%
GGAGGGACATCGCCAACTGA+AGG - chr4_3:59586134-59586153 Msa0651980:CDS 60.0%
CGCATACTCGGCTGCACGGT+TGG + chr4_3:59584867-59584886 None:intergenic 65.0%
AGTTGGCGATGTCCCTCCCC+GGG + chr4_3:59586132-59586151 None:intergenic 65.0%
CAGTTGGCGATGTCCCTCCC+CGG + chr4_3:59586133-59586152 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 59583353 59586911 59583353 ID=Msa0651980;Name=Msa0651980
chr4_3 mRNA 59583353 59586911 59583353 ID=Msa0651980-mRNA-1;Parent=Msa0651980;Name=Msa0651980-mRNA-1;_AED=0.13;_eAED=0.13;_QI=348|0.8|0.83|1|0.8|0.66|6|667|420
chr4_3 exon 59583353 59584301 59583353 ID=Msa0651980-mRNA-1:exon:15969;Parent=Msa0651980-mRNA-1
chr4_3 exon 59584611 59584687 59584611 ID=Msa0651980-mRNA-1:exon:15968;Parent=Msa0651980-mRNA-1
chr4_3 exon 59585268 59585575 59585268 ID=Msa0651980-mRNA-1:exon:15967;Parent=Msa0651980-mRNA-1
chr4_3 exon 59585676 59585776 59585676 ID=Msa0651980-mRNA-1:exon:15966;Parent=Msa0651980-mRNA-1
chr4_3 exon 59585892 59586169 59585892 ID=Msa0651980-mRNA-1:exon:15965;Parent=Msa0651980-mRNA-1
chr4_3 exon 59586347 59586911 59586347 ID=Msa0651980-mRNA-1:exon:15964;Parent=Msa0651980-mRNA-1
chr4_3 five_prime_UTR 59586564 59586911 59586564 ID=Msa0651980-mRNA-1:five_prime_utr;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59586347 59586563 59586347 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59585892 59586169 59585892 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59585676 59585776 59585676 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59585268 59585575 59585268 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59584611 59584687 59584611 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 CDS 59584020 59584301 59584020 ID=Msa0651980-mRNA-1:cds;Parent=Msa0651980-mRNA-1
chr4_3 three_prime_UTR 59583353 59584019 59583353 ID=Msa0651980-mRNA-1:three_prime_utr;Parent=Msa0651980-mRNA-1
Gene Sequence

>Msa0651980

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTAAGACCAACTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACAACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGAAAAGGGAGGAAGAAGGACAATTTGTGACTGCAGCTGAGGACCTTTTTCAATCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGTAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCGAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCACTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGACTTCATACGCGGAGAGTTCCAGCTCCAAGTGGTACCGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAAAGTCCACTTCATTTAGCTACAGGATCCCGGGGAGGGACATCGCCAACTGAAGGTGATAGCATGGACGATGACGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCAAAGACATGGAAAAGTTGGTGTATAG
Protein sequence

>Msa0651980

MGCVVPAELSLDLRPTFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEEGQFVTAAEDLFQSCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPVRNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAPSGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDDEKSESYSWKSHIQRHGKVGV*