AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0660760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660760 MtrunA17_Chr8g0377351 96.537 231 8 0 1 231 1 231 9.70e-169 464
Msa0660760 MtrunA17_Chr3g0122531 62.871 202 69 1 14 215 23 218 1.58e-92 271
Msa0660760 MtrunA17_Chr3g0122501 65.686 204 70 0 12 215 8 211 6.49e-90 264
Msa0660760 MtrunA17_Chr2g0282081 41.827 208 110 3 7 210 11 211 8.21e-49 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0660760 AT1G67360.1 52.119 236 96 2 7 225 1 236 9.03e-81 242
Msa0660760 AT1G67360.2 52.119 236 96 2 7 225 1 236 9.03e-81 242
Msa0660760 AT2G47780.1 36.321 212 118 2 10 216 34 233 3.04e-43 146
Msa0660760 AT3G05500.1 34.222 225 123 4 6 210 11 230 1.07e-39 137
Msa0660760 AT3G05500.2 29.530 149 80 4 84 212 17 160 2.19e-14 69.3

Find 60 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAAACAAATTGTGCTTAC+TGG 0.274507 4_3:-71788584 Msa0660760:CDS
GTCAGTTGTTACTGGTTATA+AGG 0.285330 4_3:+71790301 None:intergenic
CATGTTGTTAAGGACATTAC+TGG 0.302136 4_3:-71788461 Msa0660760:CDS
TGATATATAGAGGTGGAAAT+TGG 0.330039 4_3:+71790344 None:intergenic
CTTCATATTTAACTCAGAAT+TGG 0.356325 4_3:+71788158 None:intergenic
GGCTTGAATTTGTTGTAAAC+AGG 0.366243 4_3:+71789966 None:intergenic
TTCTCCTTGTTTAAATGCCT+TGG 0.368309 4_3:+71788385 None:intergenic
ATTGCTTCCTTTCTTTGTGT+TGG 0.371695 4_3:+71790262 None:intergenic
GCCTTGGCTATTTCATCAAT+AGG 0.399337 4_3:+71788401 None:intergenic
AGCATGCTCTTCAAATTTGT+TGG 0.400161 4_3:+71788739 None:intergenic
GGAAAGGGATACACACTGTT+TGG 0.405678 4_3:-71788440 Msa0660760:CDS
GCTACTTGCTTTACAAAAGT+AGG 0.431966 4_3:+71788716 None:intergenic
GCAATGTAAATTAACCTGTT+TGG 0.450709 4_3:+71790231 None:intergenic
TCTGCTGTTGGAACCGTTGA+AGG 0.466059 4_3:-71790011 Msa0660760:CDS
GGTACTGTCACTACCGTTCT+CGG 0.474596 4_3:-71789990 Msa0660760:CDS
AGCAATGACCACCACCAAAC+AGG 0.475167 4_3:-71790245 Msa0660760:intron
AAAGTACAACCATGTTGTTA+AGG 0.476497 4_3:-71788471 Msa0660760:CDS
ATCTCTGCCACTGCATGGAA+TGG 0.477336 4_3:+71788524 None:intergenic
CATGCAAACAAAAGCATGAA+TGG 0.481981 4_3:+71790079 None:intergenic
AGAGGGTGTGTAGGTATGAA+TGG 0.483484 4_3:-71788254 Msa0660760:three_prime_UTR
GTTAAGGACATTACTGGAAA+GGG 0.490360 4_3:-71788455 Msa0660760:CDS
TTACTGGTTCAGTAAAACTA+TGG 0.505586 4_3:-71788568 Msa0660760:CDS
TCTGACCAATGTGCAGCTGT+TGG 0.505736 4_3:+71788494 None:intergenic
CAAGTAGCAAATCAAGCTAA+AGG 0.511057 4_3:-71788701 Msa0660760:CDS
GTATTTCAAGATTAATACAT+TGG 0.512224 4_3:-71790397 Msa0660760:five_prime_UTR
ATAAGCTCCAACACAAAGAA+AGG 0.515995 4_3:-71790269 Msa0660760:five_prime_UTR
GTAGTGAATGAGGCTCAATC+TGG 0.522526 4_3:-71788644 Msa0660760:CDS
CAGTAATGTCCTTAACAACA+TGG 0.527456 4_3:+71788462 None:intergenic
CTGCATGGAATGGTGGATAG+TGG 0.531144 4_3:+71788534 None:intergenic
GAAAGATGTAGAGGGTGTGT+AGG 0.535625 4_3:-71788263 Msa0660760:three_prime_UTR
ACAAGGAGATCTTGAGGGAG+AGG 0.537297 4_3:+71789945 None:intergenic
GGTTCAGTAAAACTATGGTC+AGG 0.550734 4_3:-71788563 Msa0660760:CDS
TGTTAAGGACATTACTGGAA+AGG 0.554932 4_3:-71788456 Msa0660760:CDS
GGTTGGAATGTGATATATAG+AGG 0.555538 4_3:+71790334 None:intergenic
CTGCCATCTCTGCCACTGCA+TGG 0.558864 4_3:+71788519 None:intergenic
ATTTCATCAATAGGAATCAA+AGG 0.562762 4_3:+71788410 None:intergenic
GGACCTTTGAGATCTGCTGT+TGG 0.562878 4_3:-71790023 Msa0660760:CDS
CTTAATATTAACAAAGTCTG+TGG 0.564554 4_3:-71788198 Msa0660760:three_prime_UTR
AGCTGAGAAAGTAGTGAATG+AGG 0.565885 4_3:-71788654 Msa0660760:CDS
GTGAATGAGGCTCAATCTGG+TGG 0.567744 4_3:-71788641 Msa0660760:CDS
TAAATTAACCTGTTTGGTGG+TGG 0.568028 4_3:+71790237 None:intergenic
ATTCCATGCAGTGGCAGAGA+TGG 0.580335 4_3:-71788522 Msa0660760:CDS
GACTATGCAAAACAAAACTC+AGG 0.593057 4_3:-71790044 Msa0660760:CDS
TGAATGAGGCTCAATCTGGT+GGG 0.595217 4_3:-71788640 Msa0660760:CDS
TCCTATTGATGAAATAGCCA+AGG 0.598392 4_3:-71788402 Msa0660760:CDS
TCTGCCACTGCATGGAATGG+TGG 0.601951 4_3:+71788527 None:intergenic
TATAAGGAGGAAGACACGGT+TGG 0.602968 4_3:+71790317 None:intergenic
ATGTAAATTAACCTGTTTGG+TGG 0.604313 4_3:+71790234 None:intergenic
TGTGGTTGAGAAAGATGTAG+AGG 0.620908 4_3:-71788272 Msa0660760:three_prime_UTR
GTTGTAAACAGGACCGAGAA+CGG 0.624373 4_3:+71789977 None:intergenic
AGTTGTTACTGGTTATAAGG+AGG 0.626311 4_3:+71790304 None:intergenic
GTGGTTGAGAAAGATGTAGA+GGG 0.627221 4_3:-71788271 Msa0660760:three_prime_UTR
GAATGAGGCTCAATCTGGTG+GGG 0.628989 4_3:-71788639 Msa0660760:CDS
GGTAGTGACAGTACCTTCAA+CGG 0.634484 4_3:+71789998 None:intergenic
GTTCCAACAGCAGATCTCAA+AGG 0.634905 4_3:+71790020 None:intergenic
TGGTTATAAGGAGGAAGACA+CGG 0.638047 4_3:+71790313 None:intergenic
CAGTTCCAACAGCTGCACAT+TGG 0.652988 4_3:-71788499 Msa0660760:CDS
CTATCCACCATTCCATGCAG+TGG 0.660116 4_3:-71788531 Msa0660760:CDS
TGGAATGTGATATATAGAGG+TGG 0.662527 4_3:+71790337 None:intergenic
ATAGCCAAGGCATTTAAACA+AGG 0.669331 4_3:-71788389 Msa0660760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTTATTCTGATTTT+TGG - chr4_3:71788909-71788928 Msa0660760:intron 15.0%
!! TATTGAATACATAATGTATT+TGG + chr4_3:71789037-71789056 None:intergenic 15.0%
!!! TAAGCAAAAATTTACTTTTT+AGG + chr4_3:71789062-71789081 None:intergenic 15.0%
!!! TAGTTTTGTTAAAAATTCTA+TGG - chr4_3:71789183-71789202 Msa0660760:intron 15.0%
!! TATAAAGCAAAAACAAAAAT+TGG - chr4_3:71789488-71789507 Msa0660760:intron 15.0%
!!! TTGTTTTTTATTCTGATTTT+TGG - chr4_3:71788909-71788928 Msa0660760:intron 15.0%
!! TATTGAATACATAATGTATT+TGG + chr4_3:71789037-71789056 None:intergenic 15.0%
!!! TAAGCAAAAATTTACTTTTT+AGG + chr4_3:71789062-71789081 None:intergenic 15.0%
!!! TAGTTTTGTTAAAAATTCTA+TGG - chr4_3:71789183-71789202 Msa0660760:intron 15.0%
!! TATAAAGCAAAAACAAAAAT+TGG - chr4_3:71789488-71789507 Msa0660760:intron 15.0%
!! GTATTTCAAGATTAATACAT+TGG - chr4_3:71788191-71788210 Msa0660760:three_prime_UTR 20.0%
!! AACAAAAAAGCAATGAAATA+GGG + chr4_3:71788218-71788237 None:intergenic 20.0%
!! AAACAAAAAAGCAATGAAAT+AGG + chr4_3:71788219-71788238 None:intergenic 20.0%
!! TTATGAAAATTCTCAACAAA+AGG - chr4_3:71788719-71788738 Msa0660760:CDS 20.0%
!! AAAAGAAACACAAATTATCA+AGG + chr4_3:71788746-71788765 None:intergenic 20.0%
!! TGATCATTTGTAATTGAATT+CGG - chr4_3:71788783-71788802 Msa0660760:intron 20.0%
!!! TTTTTTCTTATAAATGTGAC+CGG + chr4_3:71788962-71788981 None:intergenic 20.0%
!! CATTCAATACTTAATGTATT+TGG - chr4_3:71788999-71789018 Msa0660760:intron 20.0%
!! ATTCAATCAAAGAGAATAAA+AGG + chr4_3:71789224-71789243 None:intergenic 20.0%
!!! TTTTTTTTGTACAAACTCAT+TGG - chr4_3:71789534-71789553 Msa0660760:intron 20.0%
!!! TTTTTTTGTACAAACTCATT+GGG - chr4_3:71789535-71789554 Msa0660760:intron 20.0%
!! CATAAAACAACACTTATTAT+TGG + chr4_3:71789765-71789784 None:intergenic 20.0%
!! GTATTTCAAGATTAATACAT+TGG - chr4_3:71788191-71788210 Msa0660760:three_prime_UTR 20.0%
!! AACAAAAAAGCAATGAAATA+GGG + chr4_3:71788218-71788237 None:intergenic 20.0%
!! AAACAAAAAAGCAATGAAAT+AGG + chr4_3:71788219-71788238 None:intergenic 20.0%
!! TTATGAAAATTCTCAACAAA+AGG - chr4_3:71788719-71788738 Msa0660760:CDS 20.0%
!! AAAAGAAACACAAATTATCA+AGG + chr4_3:71788746-71788765 None:intergenic 20.0%
!! TGATCATTTGTAATTGAATT+CGG - chr4_3:71788783-71788802 Msa0660760:intron 20.0%
!!! TTTTTTCTTATAAATGTGAC+CGG + chr4_3:71788962-71788981 None:intergenic 20.0%
!! CATTCAATACTTAATGTATT+TGG - chr4_3:71788999-71789018 Msa0660760:intron 20.0%
!! ATTCAATCAAAGAGAATAAA+AGG + chr4_3:71789224-71789243 None:intergenic 20.0%
!!! TTTTTTTTGTACAAACTCAT+TGG - chr4_3:71789534-71789553 Msa0660760:intron 20.0%
!!! TTTTTTTGTACAAACTCATT+GGG - chr4_3:71789535-71789554 Msa0660760:intron 20.0%
!! CATAAAACAACACTTATTAT+TGG + chr4_3:71789765-71789784 None:intergenic 20.0%
! ACAAAAAAGCAATGAAATAG+GGG + chr4_3:71788217-71788236 None:intergenic 25.0%
! ATATATTGAATTTGTGCAGA+TGG - chr4_3:71788429-71788448 Msa0660760:CDS 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr4_3:71789072-71789091 Msa0660760:intron 25.0%
!! AAATGTTGTCAGATTTTCAA+AGG + chr4_3:71789324-71789343 None:intergenic 25.0%
! TTTGAAAATCTGACAACATT+TGG - chr4_3:71789323-71789342 Msa0660760:intron 25.0%
! TTGAAAATCTGACAACATTT+GGG - chr4_3:71789324-71789343 Msa0660760:intron 25.0%
!! TTTATATCTAAAAGTGCGTT+GGG + chr4_3:71789474-71789493 None:intergenic 25.0%
! ATTTCATCAATAGGAATCAA+AGG + chr4_3:71790181-71790200 None:intergenic 25.0%
! CTTAATATTAACAAAGTCTG+TGG - chr4_3:71790390-71790409 Msa0660760:five_prime_UTR 25.0%
! CTTCATATTTAACTCAGAAT+TGG + chr4_3:71790433-71790452 None:intergenic 25.0%
!!! AAGCTTGTTTTGTTTTTTTC+AGG - chr4_3:71790450-71790469 Msa0660760:five_prime_UTR 25.0%
! ACAAAAAAGCAATGAAATAG+GGG + chr4_3:71788217-71788236 None:intergenic 25.0%
! ATATATTGAATTTGTGCAGA+TGG - chr4_3:71788429-71788448 Msa0660760:CDS 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr4_3:71789072-71789091 Msa0660760:intron 25.0%
!! AAATGTTGTCAGATTTTCAA+AGG + chr4_3:71789324-71789343 None:intergenic 25.0%
! TTTGAAAATCTGACAACATT+TGG - chr4_3:71789323-71789342 Msa0660760:intron 25.0%
! TTGAAAATCTGACAACATTT+GGG - chr4_3:71789324-71789343 Msa0660760:intron 25.0%
!! TTTATATCTAAAAGTGCGTT+GGG + chr4_3:71789474-71789493 None:intergenic 25.0%
! ATTTCATCAATAGGAATCAA+AGG + chr4_3:71790181-71790200 None:intergenic 25.0%
! CTTAATATTAACAAAGTCTG+TGG - chr4_3:71790390-71790409 Msa0660760:five_prime_UTR 25.0%
! CTTCATATTTAACTCAGAAT+TGG + chr4_3:71790433-71790452 None:intergenic 25.0%
TGATATATAGAGGTGGAAAT+TGG + chr4_3:71788247-71788266 None:intergenic 30.0%
ATGTAAATTAACCTGTTTGG+TGG + chr4_3:71788357-71788376 None:intergenic 30.0%
GCAATGTAAATTAACCTGTT+TGG + chr4_3:71788360-71788379 None:intergenic 30.0%
AAAAAACAAGGAGATCTTGA+GGG + chr4_3:71788651-71788670 None:intergenic 30.0%
AAAAAAACAAGGAGATCTTG+AGG + chr4_3:71788652-71788671 None:intergenic 30.0%
!! TTGTTTTTCCCTGATATTCA+TGG + chr4_3:71788833-71788852 None:intergenic 30.0%
AGGTTCATGTATACAAAAAG+AGG + chr4_3:71788859-71788878 None:intergenic 30.0%
TATAGATGCATGCAAATAAC+AGG + chr4_3:71788879-71788898 None:intergenic 30.0%
TCTTTGATTGAATGCATGTT+TGG - chr4_3:71789231-71789250 Msa0660760:intron 30.0%
!! TCACCACAATGATTTTGAAA+AGG - chr4_3:71789285-71789304 Msa0660760:intron 30.0%
! TAGCCTTTTCAAAATCATTG+TGG + chr4_3:71789291-71789310 None:intergenic 30.0%
TTGTGATGTCAATGTCATAT+TGG - chr4_3:71789352-71789371 Msa0660760:intron 30.0%
TGGGAAATTACATACATGTT+TGG + chr4_3:71789455-71789474 None:intergenic 30.0%
! CTTTATATCTAAAAGTGCGT+TGG + chr4_3:71789475-71789494 None:intergenic 30.0%
TTCAACATCTTATCGTTGAT+AGG + chr4_3:71789653-71789672 None:intergenic 30.0%
!!! TGTTGTTTTATGATTGTGAC+AGG - chr4_3:71789814-71789833 Msa0660760:intron 30.0%
TCTAAACAAATTGTGCTTAC+TGG - chr4_3:71790004-71790023 Msa0660760:CDS 30.0%
!! TTACTGGTTCAGTAAAACTA+TGG - chr4_3:71790020-71790039 Msa0660760:CDS 30.0%
AAAGTACAACCATGTTGTTA+AGG - chr4_3:71790117-71790136 Msa0660760:CDS 30.0%
!! GCTATTTTCTTCACATAATG+TGG - chr4_3:71790298-71790317 Msa0660760:five_prime_UTR 30.0%
TGATATATAGAGGTGGAAAT+TGG + chr4_3:71788247-71788266 None:intergenic 30.0%
ATGTAAATTAACCTGTTTGG+TGG + chr4_3:71788357-71788376 None:intergenic 30.0%
GCAATGTAAATTAACCTGTT+TGG + chr4_3:71788360-71788379 None:intergenic 30.0%
AAAAAACAAGGAGATCTTGA+GGG + chr4_3:71788651-71788670 None:intergenic 30.0%
AAAAAAACAAGGAGATCTTG+AGG + chr4_3:71788652-71788671 None:intergenic 30.0%
!! TTGTTTTTCCCTGATATTCA+TGG + chr4_3:71788833-71788852 None:intergenic 30.0%
AGGTTCATGTATACAAAAAG+AGG + chr4_3:71788859-71788878 None:intergenic 30.0%
TATAGATGCATGCAAATAAC+AGG + chr4_3:71788879-71788898 None:intergenic 30.0%
TCTTTGATTGAATGCATGTT+TGG - chr4_3:71789231-71789250 Msa0660760:intron 30.0%
!! TCACCACAATGATTTTGAAA+AGG - chr4_3:71789285-71789304 Msa0660760:intron 30.0%
! TAGCCTTTTCAAAATCATTG+TGG + chr4_3:71789291-71789310 None:intergenic 30.0%
TTGTGATGTCAATGTCATAT+TGG - chr4_3:71789352-71789371 Msa0660760:intron 30.0%
TGGGAAATTACATACATGTT+TGG + chr4_3:71789455-71789474 None:intergenic 30.0%
! CTTTATATCTAAAAGTGCGT+TGG + chr4_3:71789475-71789494 None:intergenic 30.0%
TTCAACATCTTATCGTTGAT+AGG + chr4_3:71789653-71789672 None:intergenic 30.0%
!!! TGTTGTTTTATGATTGTGAC+AGG - chr4_3:71789814-71789833 Msa0660760:intron 30.0%
TCTAAACAAATTGTGCTTAC+TGG - chr4_3:71790004-71790023 Msa0660760:CDS 30.0%
!! TTACTGGTTCAGTAAAACTA+TGG - chr4_3:71790020-71790039 Msa0660760:CDS 30.0%
AAAGTACAACCATGTTGTTA+AGG - chr4_3:71790117-71790136 Msa0660760:CDS 30.0%
!! GCTATTTTCTTCACATAATG+TGG - chr4_3:71790298-71790317 Msa0660760:five_prime_UTR 30.0%
TGGAATGTGATATATAGAGG+TGG + chr4_3:71788254-71788273 None:intergenic 35.0%
GGTTGGAATGTGATATATAG+AGG + chr4_3:71788257-71788276 None:intergenic 35.0%
! AGTTGTTACTGGTTATAAGG+AGG + chr4_3:71788287-71788306 None:intergenic 35.0%
! GTCAGTTGTTACTGGTTATA+AGG + chr4_3:71788290-71788309 None:intergenic 35.0%
! TTTTTCTCGTCAGTTGTTAC+TGG + chr4_3:71788298-71788317 None:intergenic 35.0%
ATAAGCTCCAACACAAAGAA+AGG - chr4_3:71788319-71788338 Msa0660760:exon 35.0%
ATTGCTTCCTTTCTTTGTGT+TGG + chr4_3:71788329-71788348 None:intergenic 35.0%
! TAAATTAACCTGTTTGGTGG+TGG + chr4_3:71788354-71788373 None:intergenic 35.0%
! CATGAAAAACTGAAGCATCT+TGG - chr4_3:71788469-71788488 Msa0660760:CDS 35.0%
! CATGCAAACAAAAGCATGAA+TGG + chr4_3:71788512-71788531 None:intergenic 35.0%
GACTATGCAAAACAAAACTC+AGG - chr4_3:71788544-71788563 Msa0660760:CDS 35.0%
!! GGCTTGAATTTGTTGTAAAC+AGG + chr4_3:71788625-71788644 None:intergenic 35.0%
! CCTTGTTGTCGAAAAAAACA+AGG + chr4_3:71788663-71788682 None:intergenic 35.0%
!!! CCTTGTTTTTTTCGACAACA+AGG - chr4_3:71788660-71788679 Msa0660760:CDS 35.0%
ATAAGAATATGTACTCCCTC+CGG - chr4_3:71788940-71788959 Msa0660760:intron 35.0%
TTCTTATAAATGTGACCGGA+GGG + chr4_3:71788958-71788977 None:intergenic 35.0%
TTTCTTATAAATGTGACCGG+AGG + chr4_3:71788959-71788978 None:intergenic 35.0%
AACTTATTAGTTACTCCCTC+CGG + chr4_3:71789094-71789113 None:intergenic 35.0%
TGAATGCATGTTTGGTATCA+CGG - chr4_3:71789239-71789258 Msa0660760:intron 35.0%
!! GATTTTCAAGGACAGCATTT+GGG + chr4_3:71789391-71789410 None:intergenic 35.0%
!! TGATTTTCAAGGACAGCATT+TGG + chr4_3:71789392-71789411 None:intergenic 35.0%
AATGCTGTCCTTGAAAATCA+CGG - chr4_3:71789392-71789411 Msa0660760:intron 35.0%
ATTTGTTTGTCAAAACCACG+CGG + chr4_3:71789425-71789444 None:intergenic 35.0%
ATTCTCAATGAGTTAGGTGT+GGG + chr4_3:71789601-71789620 None:intergenic 35.0%
AATTCTCAATGAGTTAGGTG+TGG + chr4_3:71789602-71789621 None:intergenic 35.0%
ACCTAACTCATTGAGAATTG+AGG - chr4_3:71789603-71789622 Msa0660760:intron 35.0%
! TGTTTTATGATTGTGACAGG+TGG - chr4_3:71789817-71789836 Msa0660760:intron 35.0%
AGCATGCTCTTCAAATTTGT+TGG + chr4_3:71789852-71789871 None:intergenic 35.0%
GCTACTTGCTTTACAAAAGT+AGG + chr4_3:71789875-71789894 None:intergenic 35.0%
CAAGTAGCAAATCAAGCTAA+AGG - chr4_3:71789887-71789906 Msa0660760:intron 35.0%
CAGTAATGTCCTTAACAACA+TGG + chr4_3:71790129-71790148 None:intergenic 35.0%
CATGTTGTTAAGGACATTAC+TGG - chr4_3:71790127-71790146 Msa0660760:CDS 35.0%
TGTTAAGGACATTACTGGAA+AGG - chr4_3:71790132-71790151 Msa0660760:CDS 35.0%
GTTAAGGACATTACTGGAAA+GGG - chr4_3:71790133-71790152 Msa0660760:CDS 35.0%
TCCTATTGATGAAATAGCCA+AGG - chr4_3:71790186-71790205 Msa0660760:intron 35.0%
! ATAGCCAAGGCATTTAAACA+AGG - chr4_3:71790199-71790218 Msa0660760:intron 35.0%
! TTCTCCTTGTTTAAATGCCT+TGG + chr4_3:71790206-71790225 None:intergenic 35.0%
TGGAATGTGATATATAGAGG+TGG + chr4_3:71788254-71788273 None:intergenic 35.0%
GGTTGGAATGTGATATATAG+AGG + chr4_3:71788257-71788276 None:intergenic 35.0%
! AGTTGTTACTGGTTATAAGG+AGG + chr4_3:71788287-71788306 None:intergenic 35.0%
! GTCAGTTGTTACTGGTTATA+AGG + chr4_3:71788290-71788309 None:intergenic 35.0%
! TTTTTCTCGTCAGTTGTTAC+TGG + chr4_3:71788298-71788317 None:intergenic 35.0%
ATAAGCTCCAACACAAAGAA+AGG - chr4_3:71788319-71788338 Msa0660760:exon 35.0%
ATTGCTTCCTTTCTTTGTGT+TGG + chr4_3:71788329-71788348 None:intergenic 35.0%
! TAAATTAACCTGTTTGGTGG+TGG + chr4_3:71788354-71788373 None:intergenic 35.0%
! CATGAAAAACTGAAGCATCT+TGG - chr4_3:71788469-71788488 Msa0660760:CDS 35.0%
! CATGCAAACAAAAGCATGAA+TGG + chr4_3:71788512-71788531 None:intergenic 35.0%
GACTATGCAAAACAAAACTC+AGG - chr4_3:71788544-71788563 Msa0660760:CDS 35.0%
!! GGCTTGAATTTGTTGTAAAC+AGG + chr4_3:71788625-71788644 None:intergenic 35.0%
! CCTTGTTGTCGAAAAAAACA+AGG + chr4_3:71788663-71788682 None:intergenic 35.0%
!!! CCTTGTTTTTTTCGACAACA+AGG - chr4_3:71788660-71788679 Msa0660760:CDS 35.0%
ATAAGAATATGTACTCCCTC+CGG - chr4_3:71788940-71788959 Msa0660760:intron 35.0%
TTCTTATAAATGTGACCGGA+GGG + chr4_3:71788958-71788977 None:intergenic 35.0%
TTTCTTATAAATGTGACCGG+AGG + chr4_3:71788959-71788978 None:intergenic 35.0%
AACTTATTAGTTACTCCCTC+CGG + chr4_3:71789094-71789113 None:intergenic 35.0%
TGAATGCATGTTTGGTATCA+CGG - chr4_3:71789239-71789258 Msa0660760:intron 35.0%
!! GATTTTCAAGGACAGCATTT+GGG + chr4_3:71789391-71789410 None:intergenic 35.0%
!! TGATTTTCAAGGACAGCATT+TGG + chr4_3:71789392-71789411 None:intergenic 35.0%
AATGCTGTCCTTGAAAATCA+CGG - chr4_3:71789392-71789411 Msa0660760:intron 35.0%
ATTTGTTTGTCAAAACCACG+CGG + chr4_3:71789425-71789444 None:intergenic 35.0%
ATTCTCAATGAGTTAGGTGT+GGG + chr4_3:71789601-71789620 None:intergenic 35.0%
AATTCTCAATGAGTTAGGTG+TGG + chr4_3:71789602-71789621 None:intergenic 35.0%
ACCTAACTCATTGAGAATTG+AGG - chr4_3:71789603-71789622 Msa0660760:intron 35.0%
! TGTTTTATGATTGTGACAGG+TGG - chr4_3:71789817-71789836 Msa0660760:intron 35.0%
AGCATGCTCTTCAAATTTGT+TGG + chr4_3:71789852-71789871 None:intergenic 35.0%
GCTACTTGCTTTACAAAAGT+AGG + chr4_3:71789875-71789894 None:intergenic 35.0%
CAAGTAGCAAATCAAGCTAA+AGG - chr4_3:71789887-71789906 Msa0660760:intron 35.0%
CAGTAATGTCCTTAACAACA+TGG + chr4_3:71790129-71790148 None:intergenic 35.0%
CATGTTGTTAAGGACATTAC+TGG - chr4_3:71790127-71790146 Msa0660760:CDS 35.0%
TGTTAAGGACATTACTGGAA+AGG - chr4_3:71790132-71790151 Msa0660760:CDS 35.0%
GTTAAGGACATTACTGGAAA+GGG - chr4_3:71790133-71790152 Msa0660760:CDS 35.0%
TCCTATTGATGAAATAGCCA+AGG - chr4_3:71790186-71790205 Msa0660760:intron 35.0%
! ATAGCCAAGGCATTTAAACA+AGG - chr4_3:71790199-71790218 Msa0660760:intron 35.0%
! TTCTCCTTGTTTAAATGCCT+TGG + chr4_3:71790206-71790225 None:intergenic 35.0%
TGGTTATAAGGAGGAAGACA+CGG + chr4_3:71788278-71788297 None:intergenic 40.0%
TACGTTCTGCCATGAATATC+AGG - chr4_3:71788821-71788840 Msa0660760:intron 40.0%
ACGTTCTGCCATGAATATCA+GGG - chr4_3:71788822-71788841 Msa0660760:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr4_3:71789075-71789094 Msa0660760:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr4_3:71789076-71789095 Msa0660760:intron 40.0%
TGCATGCATGTCAAGTTTCA+AGG + chr4_3:71789136-71789155 None:intergenic 40.0%
AGCATTTGGGTGACATTGTA+GGG + chr4_3:71789378-71789397 None:intergenic 40.0%
GCCTCAATTCTCAATGAGTT+AGG + chr4_3:71789607-71789626 None:intergenic 40.0%
! CAAACTGCAGTTGGTACATA+TGG + chr4_3:71789719-71789738 None:intergenic 40.0%
ATGTACCAACTGCAGTTTGT+TGG - chr4_3:71789720-71789739 Msa0660760:intron 40.0%
TATCACCAACAAACTGCAGT+TGG + chr4_3:71789728-71789747 None:intergenic 40.0%
AGCTGAGAAAGTAGTGAATG+AGG - chr4_3:71789934-71789953 Msa0660760:CDS 40.0%
GGTTCAGTAAAACTATGGTC+AGG - chr4_3:71790025-71790044 Msa0660760:CDS 40.0%
! GCCTTGGCTATTTCATCAAT+AGG + chr4_3:71790190-71790209 None:intergenic 40.0%
TGTGGTTGAGAAAGATGTAG+AGG - chr4_3:71790316-71790335 Msa0660760:five_prime_UTR 40.0%
GTGGTTGAGAAAGATGTAGA+GGG - chr4_3:71790317-71790336 Msa0660760:five_prime_UTR 40.0%
TGGTTATAAGGAGGAAGACA+CGG + chr4_3:71788278-71788297 None:intergenic 40.0%
TACGTTCTGCCATGAATATC+AGG - chr4_3:71788821-71788840 Msa0660760:intron 40.0%
ACGTTCTGCCATGAATATCA+GGG - chr4_3:71788822-71788841 Msa0660760:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr4_3:71789075-71789094 Msa0660760:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr4_3:71789076-71789095 Msa0660760:intron 40.0%
TGCATGCATGTCAAGTTTCA+AGG + chr4_3:71789136-71789155 None:intergenic 40.0%
AGCATTTGGGTGACATTGTA+GGG + chr4_3:71789378-71789397 None:intergenic 40.0%
GCCTCAATTCTCAATGAGTT+AGG + chr4_3:71789607-71789626 None:intergenic 40.0%
! CAAACTGCAGTTGGTACATA+TGG + chr4_3:71789719-71789738 None:intergenic 40.0%
ATGTACCAACTGCAGTTTGT+TGG - chr4_3:71789720-71789739 Msa0660760:intron 40.0%
TATCACCAACAAACTGCAGT+TGG + chr4_3:71789728-71789747 None:intergenic 40.0%
AGCTGAGAAAGTAGTGAATG+AGG - chr4_3:71789934-71789953 Msa0660760:CDS 40.0%
GGTTCAGTAAAACTATGGTC+AGG - chr4_3:71790025-71790044 Msa0660760:CDS 40.0%
! GCCTTGGCTATTTCATCAAT+AGG + chr4_3:71790190-71790209 None:intergenic 40.0%
TGTGGTTGAGAAAGATGTAG+AGG - chr4_3:71790316-71790335 Msa0660760:five_prime_UTR 40.0%
GTGGTTGAGAAAGATGTAGA+GGG - chr4_3:71790317-71790336 Msa0660760:five_prime_UTR 40.0%
TATAAGGAGGAAGACACGGT+TGG + chr4_3:71788274-71788293 None:intergenic 45.0%
GTTCCAACAGCAGATCTCAA+AGG + chr4_3:71788571-71788590 None:intergenic 45.0%
GGTAGTGACAGTACCTTCAA+CGG + chr4_3:71788593-71788612 None:intergenic 45.0%
GTTGTAAACAGGACCGAGAA+CGG + chr4_3:71788614-71788633 None:intergenic 45.0%
CAGCATTTGGGTGACATTGT+AGG + chr4_3:71789379-71789398 None:intergenic 45.0%
! GTGAGTCACCGTGATTTTCA+AGG + chr4_3:71789403-71789422 None:intergenic 45.0%
TCAATGAGTTAGGTGTGGGA+TGG + chr4_3:71789597-71789616 None:intergenic 45.0%
GTTCACATGCGATGTGACTT+GGG + chr4_3:71789682-71789701 None:intergenic 45.0%
CAAGTCACATCGCATGTGAA+CGG - chr4_3:71789681-71789700 Msa0660760:intron 45.0%
! CTGCAGTTGGTACATATGGT+TGG + chr4_3:71789715-71789734 None:intergenic 45.0%
GTAGTGAATGAGGCTCAATC+TGG - chr4_3:71789944-71789963 Msa0660760:CDS 45.0%
TGAATGAGGCTCAATCTGGT+GGG - chr4_3:71789948-71789967 Msa0660760:CDS 45.0%
GGAAAGGGATACACACTGTT+TGG - chr4_3:71790148-71790167 Msa0660760:intron 45.0%
! GAAAGATGTAGAGGGTGTGT+AGG - chr4_3:71790325-71790344 Msa0660760:five_prime_UTR 45.0%
! AGAGGGTGTGTAGGTATGAA+TGG - chr4_3:71790334-71790353 Msa0660760:five_prime_UTR 45.0%
TATAAGGAGGAAGACACGGT+TGG + chr4_3:71788274-71788293 None:intergenic 45.0%
GTTCCAACAGCAGATCTCAA+AGG + chr4_3:71788571-71788590 None:intergenic 45.0%
GGTAGTGACAGTACCTTCAA+CGG + chr4_3:71788593-71788612 None:intergenic 45.0%
GTTGTAAACAGGACCGAGAA+CGG + chr4_3:71788614-71788633 None:intergenic 45.0%
CAGCATTTGGGTGACATTGT+AGG + chr4_3:71789379-71789398 None:intergenic 45.0%
! GTGAGTCACCGTGATTTTCA+AGG + chr4_3:71789403-71789422 None:intergenic 45.0%
TCAATGAGTTAGGTGTGGGA+TGG + chr4_3:71789597-71789616 None:intergenic 45.0%
GTTCACATGCGATGTGACTT+GGG + chr4_3:71789682-71789701 None:intergenic 45.0%
CAAGTCACATCGCATGTGAA+CGG - chr4_3:71789681-71789700 Msa0660760:intron 45.0%
! CTGCAGTTGGTACATATGGT+TGG + chr4_3:71789715-71789734 None:intergenic 45.0%
GTAGTGAATGAGGCTCAATC+TGG - chr4_3:71789944-71789963 Msa0660760:CDS 45.0%
TGAATGAGGCTCAATCTGGT+GGG - chr4_3:71789948-71789967 Msa0660760:CDS 45.0%
GGAAAGGGATACACACTGTT+TGG - chr4_3:71790148-71790167 Msa0660760:intron 45.0%
! GAAAGATGTAGAGGGTGTGT+AGG - chr4_3:71790325-71790344 Msa0660760:five_prime_UTR 45.0%
! AGAGGGTGTGTAGGTATGAA+TGG - chr4_3:71790334-71790353 Msa0660760:five_prime_UTR 45.0%
AGCAATGACCACCACCAAAC+AGG - chr4_3:71788343-71788362 Msa0660760:CDS 50.0%
!! GGACCTTTGAGATCTGCTGT+TGG - chr4_3:71788565-71788584 Msa0660760:CDS 50.0%
TCTGCTGTTGGAACCGTTGA+AGG - chr4_3:71788577-71788596 Msa0660760:CDS 50.0%
GGTACTGTCACTACCGTTCT+CGG - chr4_3:71788598-71788617 Msa0660760:CDS 50.0%
ACAAGGAGATCTTGAGGGAG+AGG + chr4_3:71788646-71788665 None:intergenic 50.0%
TGAGTTAGGTGTGGGATGGT+AGG + chr4_3:71789593-71789612 None:intergenic 50.0%
CGTTCACATGCGATGTGACT+TGG + chr4_3:71789683-71789702 None:intergenic 50.0%
GTGAATGAGGCTCAATCTGG+TGG - chr4_3:71789947-71789966 Msa0660760:CDS 50.0%
GAATGAGGCTCAATCTGGTG+GGG - chr4_3:71789949-71789968 Msa0660760:CDS 50.0%
!! CTGCATGGAATGGTGGATAG+TGG + chr4_3:71790057-71790076 None:intergenic 50.0%
CTATCCACCATTCCATGCAG+TGG - chr4_3:71790057-71790076 Msa0660760:CDS 50.0%
ATCTCTGCCACTGCATGGAA+TGG + chr4_3:71790067-71790086 None:intergenic 50.0%
ATTCCATGCAGTGGCAGAGA+TGG - chr4_3:71790066-71790085 Msa0660760:CDS 50.0%
CAGTTCCAACAGCTGCACAT+TGG - chr4_3:71790089-71790108 Msa0660760:CDS 50.0%
TCTGACCAATGTGCAGCTGT+TGG + chr4_3:71790097-71790116 None:intergenic 50.0%
AGCAATGACCACCACCAAAC+AGG - chr4_3:71788343-71788362 Msa0660760:CDS 50.0%
!! GGACCTTTGAGATCTGCTGT+TGG - chr4_3:71788565-71788584 Msa0660760:CDS 50.0%
TCTGCTGTTGGAACCGTTGA+AGG - chr4_3:71788577-71788596 Msa0660760:CDS 50.0%
GGTACTGTCACTACCGTTCT+CGG - chr4_3:71788598-71788617 Msa0660760:CDS 50.0%
ACAAGGAGATCTTGAGGGAG+AGG + chr4_3:71788646-71788665 None:intergenic 50.0%
TGAGTTAGGTGTGGGATGGT+AGG + chr4_3:71789593-71789612 None:intergenic 50.0%
CGTTCACATGCGATGTGACT+TGG + chr4_3:71789683-71789702 None:intergenic 50.0%
GTGAATGAGGCTCAATCTGG+TGG - chr4_3:71789947-71789966 Msa0660760:CDS 50.0%
GAATGAGGCTCAATCTGGTG+GGG - chr4_3:71789949-71789968 Msa0660760:CDS 50.0%
!! CTGCATGGAATGGTGGATAG+TGG + chr4_3:71790057-71790076 None:intergenic 50.0%
CTATCCACCATTCCATGCAG+TGG - chr4_3:71790057-71790076 Msa0660760:CDS 50.0%
ATCTCTGCCACTGCATGGAA+TGG + chr4_3:71790067-71790086 None:intergenic 50.0%
ATTCCATGCAGTGGCAGAGA+TGG - chr4_3:71790066-71790085 Msa0660760:CDS 50.0%
CAGTTCCAACAGCTGCACAT+TGG - chr4_3:71790089-71790108 Msa0660760:CDS 50.0%
TCTGACCAATGTGCAGCTGT+TGG + chr4_3:71790097-71790116 None:intergenic 50.0%
TCTGCCACTGCATGGAATGG+TGG + chr4_3:71790064-71790083 None:intergenic 55.0%
TCTGCCACTGCATGGAATGG+TGG + chr4_3:71790064-71790083 None:intergenic 55.0%
AATCACGGTGACTCACCGCG+TGG - chr4_3:71789407-71789426 Msa0660760:intron 60.0%
CTGCCATCTCTGCCACTGCA+TGG + chr4_3:71790072-71790091 None:intergenic 60.0%
AATCACGGTGACTCACCGCG+TGG - chr4_3:71789407-71789426 Msa0660760:intron 60.0%
CTGCCATCTCTGCCACTGCA+TGG + chr4_3:71790072-71790091 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 71788141 71790469 71788141 ID=Msa0660760;Name=Msa0660760
chr4_3 mRNA 71788141 71790469 71788141 ID=Msa0660760-mRNA-1;Parent=Msa0660760;Name=Msa0660760-mRNA-1;_AED=0.02;_eAED=0.02;_QI=206|1|1|1|1|1|3|190|231
chr4_3 exon 71788141 71788774 71788141 ID=Msa0660760-mRNA-1:exon:21487;Parent=Msa0660760-mRNA-1
chr4_3 exon 71789929 71790162 71789929 ID=Msa0660760-mRNA-1:exon:21486;Parent=Msa0660760-mRNA-1
chr4_3 exon 71790246 71790469 71790246 ID=Msa0660760-mRNA-1:exon:21485;Parent=Msa0660760-mRNA-1
chr4_3 five_prime_UTR 71790264 71790469 71790264 ID=Msa0660760-mRNA-1:five_prime_utr;Parent=Msa0660760-mRNA-1
chr4_3 CDS 71790246 71790263 71790246 ID=Msa0660760-mRNA-1:cds;Parent=Msa0660760-mRNA-1
chr4_3 CDS 71789929 71790162 71789929 ID=Msa0660760-mRNA-1:cds;Parent=Msa0660760-mRNA-1
chr4_3 CDS 71788331 71788774 71788331 ID=Msa0660760-mRNA-1:cds;Parent=Msa0660760-mRNA-1
chr4_3 three_prime_UTR 71788141 71788330 71788141 ID=Msa0660760-mRNA-1:three_prime_utr;Parent=Msa0660760-mRNA-1
Gene Sequence

>Msa0660760

ATGACCACCACCAAACAGATGGATGTAGAGAGAAATGATCATGAAAAACTGAAGCATCTTGGATTTGTGAGAATTGCTGCCATTCATGCTTTTGTTTGCATGACGAGTCTGTATGACTATGCAAAACAAAACTCAGGACCTTTGAGATCTGCTGTTGGAACCGTTGAAGGTACTGTCACTACCGTTCTCGGTCCTGTTTACAACAAATTCAAGCCTCTCCCTCAAGATCTCCTTGTTTTTTTCGACAACAAGGTGGATGAAGCAACCAACAAATTTGAAGAGCATGCTCCTACTTTTGTAAAGCAAGTAGCAAATCAAGCTAAAGGTTTGATTCAAGAAGCGACACACAAAGCTGAGAAAGTAGTGAATGAGGCTCAATCTGGTGGGGCAAAAGCAGCTGCAAATTATGTAGCTACAGAATCTAAACAAATTGTGCTTACTGGTTCAGTAAAACTATGGTCAGGTCTCAACCACTATCCACCATTCCATGCAGTGGCAGAGATGGCAGTTCCAACAGCTGCACATTGGTCAGAAAAGTACAACCATGTTGTTAAGGACATTACTGGAAAGGGATACACACTGTTTGGATATTTGCCTTTGATTCCTATTGATGAAATAGCCAAGGCATTTAAACAAGGAGAAGTTAATGTGAGTGCAGATCATGAAACATCTTCTGTTGAAGATTTTTCTGATTGA
Protein sequence

>Msa0660760

MTTTKQMDVERNDHEKLKHLGFVRIAAIHAFVCMTSLYDYAKQNSGPLRSAVGTVEGTVTTVLGPVYNKFKPLPQDLLVFFDNKVDEATNKFEEHAPTFVKQVANQAKGLIQEATHKAEKVVNEAQSGGAKAAANYVATESKQIVLTGSVKLWSGLNHYPPFHAVAEMAVPTAAHWSEKYNHVVKDITGKGYTLFGYLPLIPIDEIAKAFKQGEVNVSADHETSSVEDFSD*