AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1085480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1085480 MtrunA17_Chr8g0377351 96.537 231 8 0 1 231 1 231 7.87e-169 464
Msa1085480 MtrunA17_Chr3g0122531 62.871 202 69 1 14 215 23 218 1.30e-92 271
Msa1085480 MtrunA17_Chr3g0122501 65.686 204 70 0 12 215 8 211 6.79e-90 264
Msa1085480 MtrunA17_Chr2g0282081 41.346 208 111 3 7 210 11 211 1.51e-48 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1085480 AT1G67360.1 52.119 236 96 2 7 225 1 236 8.01e-81 242
Msa1085480 AT1G67360.2 52.119 236 96 2 7 225 1 236 8.01e-81 242
Msa1085480 AT2G47780.1 36.321 212 118 2 10 216 34 233 2.35e-43 146
Msa1085480 AT3G05500.1 33.778 225 124 4 6 210 11 230 1.66e-39 137
Msa1085480 AT3G05500.2 28.859 149 81 4 84 212 17 160 5.41e-14 68.2

Find 60 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAAACAAATTGTGCTTAC+TGG 0.274507 7_2:-86248278 Msa1085480:CDS
GTCAGTTGTTACTGGTTATA+AGG 0.285330 7_2:+86249992 None:intergenic
CATGTTGTTAAGGACATTAC+TGG 0.302136 7_2:-86248155 Msa1085480:CDS
TGATATATAGAGGTGGAAAT+TGG 0.330039 7_2:+86250035 None:intergenic
CTTCATATTTAACTCAGAAT+TGG 0.356325 7_2:+86247852 None:intergenic
GGCTTGAATTTGTTGTAAAC+AGG 0.366243 7_2:+86249657 None:intergenic
TTCTCCTTGTTTAAATGCCT+TGG 0.368309 7_2:+86248079 None:intergenic
ATTGCTTCCTTTCTTTGTGT+TGG 0.371695 7_2:+86249953 None:intergenic
GCCTTGGCTATTTCATCAAT+AGG 0.399337 7_2:+86248095 None:intergenic
AGCATGCTCTTCAAATTTGT+TGG 0.400161 7_2:+86248433 None:intergenic
GGAAAGGGATACACACTGTT+TGG 0.405678 7_2:-86248134 Msa1085480:CDS
GCTACTTGCTTTACAAAAGT+AGG 0.431966 7_2:+86248410 None:intergenic
GCAATGTAAATTAACCTGTT+TGG 0.450709 7_2:+86249922 None:intergenic
TCTGCTGTTGGAACCGTTGA+AGG 0.466059 7_2:-86249702 Msa1085480:CDS
CATGCAAACAAAAGCATGAA+TGG 0.471915 7_2:+86249770 None:intergenic
GGTACTGTCACTACCGTTCT+CGG 0.474596 7_2:-86249681 Msa1085480:CDS
AGCAATGACCACCACCAAAC+AGG 0.475167 7_2:-86249936 Msa1085480:intron
AAAGTACAACCATGTTGTTA+AGG 0.476497 7_2:-86248165 Msa1085480:CDS
ATCTCTGCCACTGCATGGAA+TGG 0.477336 7_2:+86248218 None:intergenic
AGAGGGTGTGTAGGTATGAA+TGG 0.483484 7_2:-86247948 Msa1085480:three_prime_UTR
GTTAAGGACATTACTGGAAA+GGG 0.490360 7_2:-86248149 Msa1085480:CDS
TTACTGGTTCAGTAAAACTA+TGG 0.505586 7_2:-86248262 Msa1085480:CDS
TCTGACCAATGTGCAGCTGT+TGG 0.505736 7_2:+86248188 None:intergenic
CAAGTAGCAAATCAAGCTAA+AGG 0.511057 7_2:-86248395 Msa1085480:CDS
GTATTTCAAGATTAATACAT+TGG 0.512224 7_2:-86250088 Msa1085480:five_prime_UTR
ATAAGCTCCAACACAAAGAA+AGG 0.515995 7_2:-86249960 Msa1085480:five_prime_UTR
GTAGTGAATGAGGCTCAATC+TGG 0.522526 7_2:-86248338 Msa1085480:CDS
CAGTAATGTCCTTAACAACA+TGG 0.527456 7_2:+86248156 None:intergenic
CTGCATGGAATGGTGGATAG+TGG 0.531144 7_2:+86248228 None:intergenic
GAAAGATGTAGAGGGTGTGT+AGG 0.535625 7_2:-86247957 Msa1085480:three_prime_UTR
ACAAGGAGATCTTGAGGGAG+AGG 0.537297 7_2:+86249636 None:intergenic
TGTTAAGGACATTACTGGAA+AGG 0.554932 7_2:-86248150 Msa1085480:CDS
GGTTGGAATGTGATATATAG+AGG 0.555538 7_2:+86250025 None:intergenic
CTGCCATCTCTGCCACTGCA+TGG 0.558864 7_2:+86248213 None:intergenic
ATTTCATCAATAGGAATCAA+AGG 0.562762 7_2:+86248104 None:intergenic
GGACCTTTGAGATCTGCTGT+TGG 0.562878 7_2:-86249714 Msa1085480:CDS
CTTAATATTAACAAAGTCTG+TGG 0.564554 7_2:-86247892 Msa1085480:three_prime_UTR
GGTTCAGTAAAACTATGGTC+AGG 0.565308 7_2:-86248257 Msa1085480:CDS
AGCTGAGAAAGTAGTGAATG+AGG 0.565885 7_2:-86248348 Msa1085480:CDS
GTGAATGAGGCTCAATCTGG+TGG 0.567744 7_2:-86248335 Msa1085480:CDS
TAAATTAACCTGTTTGGTGG+TGG 0.568028 7_2:+86249928 None:intergenic
ATTCCATGCAGTGGCAGAGA+TGG 0.580335 7_2:-86248216 Msa1085480:CDS
GACTATGCAAAACAAAACTC+AGG 0.593057 7_2:-86249735 Msa1085480:CDS
TGAATGAGGCTCAATCTGGT+GGG 0.595217 7_2:-86248334 Msa1085480:CDS
TCCTATTGATGAAATAGCCA+AGG 0.598392 7_2:-86248096 Msa1085480:CDS
TCTGCCACTGCATGGAATGG+TGG 0.601951 7_2:+86248221 None:intergenic
TATAAGGAGGAAGACACGGT+TGG 0.602968 7_2:+86250008 None:intergenic
ATGTAAATTAACCTGTTTGG+TGG 0.604313 7_2:+86249925 None:intergenic
TGTGGTTGAGAAAGATGTAG+AGG 0.620908 7_2:-86247966 Msa1085480:three_prime_UTR
GTTGTAAACAGGACCGAGAA+CGG 0.624373 7_2:+86249668 None:intergenic
AGTTGTTACTGGTTATAAGG+AGG 0.626311 7_2:+86249995 None:intergenic
GTGGTTGAGAAAGATGTAGA+GGG 0.627221 7_2:-86247965 Msa1085480:three_prime_UTR
GAATGAGGCTCAATCTGGTG+GGG 0.628989 7_2:-86248333 Msa1085480:CDS
GGTAGTGACAGTACCTTCAA+CGG 0.634484 7_2:+86249689 None:intergenic
GTTCCAACAGCAGATCTCAA+AGG 0.634905 7_2:+86249711 None:intergenic
TGGTTATAAGGAGGAAGACA+CGG 0.638047 7_2:+86250004 None:intergenic
CAGTTCCAACAGCTGCACAT+TGG 0.652988 7_2:-86248193 Msa1085480:CDS
CTATCCACCATTCCATGCAG+TGG 0.660116 7_2:-86248225 Msa1085480:CDS
TGGAATGTGATATATAGAGG+TGG 0.662527 7_2:+86250028 None:intergenic
ATAGCCAAGGCATTTAAACA+AGG 0.669331 7_2:-86248083 Msa1085480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTTATTCTGATTTT+TGG - chr7_2:86248603-86248622 Msa1085480:intron 15.0%
!!! TAAGCAAAAATTTACTTTTT+AGG + chr7_2:86248755-86248774 None:intergenic 15.0%
!!! TAGTTTTGTTAAAAATTCTA+TGG - chr7_2:86248876-86248895 Msa1085480:intron 15.0%
!! TATAAAGCAAAAACAAAAAT+TGG - chr7_2:86249181-86249200 Msa1085480:intron 15.0%
!!! TTGTTTTTTATTCTGATTTT+TGG - chr7_2:86248603-86248622 Msa1085480:intron 15.0%
!!! TAAGCAAAAATTTACTTTTT+AGG + chr7_2:86248755-86248774 None:intergenic 15.0%
!!! TAGTTTTGTTAAAAATTCTA+TGG - chr7_2:86248876-86248895 Msa1085480:intron 15.0%
!! TATAAAGCAAAAACAAAAAT+TGG - chr7_2:86249181-86249200 Msa1085480:intron 15.0%
!! GTATTTCAAGATTAATACAT+TGG - chr7_2:86247885-86247904 Msa1085480:three_prime_UTR 20.0%
!! AACAAAAAAGCAATGAAATA+GGG + chr7_2:86247912-86247931 None:intergenic 20.0%
!! AAACAAAAAAGCAATGAAAT+AGG + chr7_2:86247913-86247932 None:intergenic 20.0%
!! TTATGAAAATTCTCAACAAA+AGG - chr7_2:86248413-86248432 Msa1085480:CDS 20.0%
!! AAAAGAAACACAAATTATCA+AGG + chr7_2:86248440-86248459 None:intergenic 20.0%
!! TGATCATTTGTAATTGAATT+CGG - chr7_2:86248477-86248496 Msa1085480:intron 20.0%
!!! TTTTTTCTTATAAATGTGAC+CGG + chr7_2:86248656-86248675 None:intergenic 20.0%
!! CATTCAATACTTAATGTATT+TGG - chr7_2:86248692-86248711 Msa1085480:intron 20.0%
!! ATTCAATCAAAGAGAATAAA+AGG + chr7_2:86248917-86248936 None:intergenic 20.0%
!!! ATTTTTTTGTACAAACTCAT+TGG - chr7_2:86249226-86249245 Msa1085480:intron 20.0%
!!! TTTTTTTGTACAAACTCATT+GGG - chr7_2:86249227-86249246 Msa1085480:intron 20.0%
!! CATAAAACAACACTTATTAT+TGG + chr7_2:86249457-86249476 None:intergenic 20.0%
!! GTATTTCAAGATTAATACAT+TGG - chr7_2:86247885-86247904 Msa1085480:three_prime_UTR 20.0%
!! AACAAAAAAGCAATGAAATA+GGG + chr7_2:86247912-86247931 None:intergenic 20.0%
!! AAACAAAAAAGCAATGAAAT+AGG + chr7_2:86247913-86247932 None:intergenic 20.0%
!! TTATGAAAATTCTCAACAAA+AGG - chr7_2:86248413-86248432 Msa1085480:CDS 20.0%
!! AAAAGAAACACAAATTATCA+AGG + chr7_2:86248440-86248459 None:intergenic 20.0%
!! TGATCATTTGTAATTGAATT+CGG - chr7_2:86248477-86248496 Msa1085480:intron 20.0%
!!! TTTTTTCTTATAAATGTGAC+CGG + chr7_2:86248656-86248675 None:intergenic 20.0%
!! CATTCAATACTTAATGTATT+TGG - chr7_2:86248692-86248711 Msa1085480:intron 20.0%
!! ATTCAATCAAAGAGAATAAA+AGG + chr7_2:86248917-86248936 None:intergenic 20.0%
!!! ATTTTTTTGTACAAACTCAT+TGG - chr7_2:86249226-86249245 Msa1085480:intron 20.0%
!!! TTTTTTTGTACAAACTCATT+GGG - chr7_2:86249227-86249246 Msa1085480:intron 20.0%
!! CATAAAACAACACTTATTAT+TGG + chr7_2:86249457-86249476 None:intergenic 20.0%
! ACAAAAAAGCAATGAAATAG+GGG + chr7_2:86247911-86247930 None:intergenic 25.0%
! ATATATTGAATTTGTGCAGA+TGG - chr7_2:86248123-86248142 Msa1085480:CDS 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr7_2:86248765-86248784 Msa1085480:intron 25.0%
!! TTTATATCTAAAAGTGCGTT+GGG + chr7_2:86249167-86249186 None:intergenic 25.0%
! ATTTCATCAATAGGAATCAA+AGG + chr7_2:86249872-86249891 None:intergenic 25.0%
! CTTAATATTAACAAAGTCTG+TGG - chr7_2:86250081-86250100 Msa1085480:five_prime_UTR 25.0%
! CTTCATATTTAACTCAGAAT+TGG + chr7_2:86250124-86250143 None:intergenic 25.0%
!!! AAGCTTGTTTTGTTTTTTTC+AGG - chr7_2:86250141-86250160 Msa1085480:five_prime_UTR 25.0%
! ACAAAAAAGCAATGAAATAG+GGG + chr7_2:86247911-86247930 None:intergenic 25.0%
! ATATATTGAATTTGTGCAGA+TGG - chr7_2:86248123-86248142 Msa1085480:CDS 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr7_2:86248765-86248784 Msa1085480:intron 25.0%
!! TTTATATCTAAAAGTGCGTT+GGG + chr7_2:86249167-86249186 None:intergenic 25.0%
! ATTTCATCAATAGGAATCAA+AGG + chr7_2:86249872-86249891 None:intergenic 25.0%
! CTTAATATTAACAAAGTCTG+TGG - chr7_2:86250081-86250100 Msa1085480:five_prime_UTR 25.0%
! CTTCATATTTAACTCAGAAT+TGG + chr7_2:86250124-86250143 None:intergenic 25.0%
TGATATATAGAGGTGGAAAT+TGG + chr7_2:86247941-86247960 None:intergenic 30.0%
ATGTAAATTAACCTGTTTGG+TGG + chr7_2:86248051-86248070 None:intergenic 30.0%
GCAATGTAAATTAACCTGTT+TGG + chr7_2:86248054-86248073 None:intergenic 30.0%
AAAAAACAAGGAGATCTTGA+GGG + chr7_2:86248345-86248364 None:intergenic 30.0%
AAAAAAACAAGGAGATCTTG+AGG + chr7_2:86248346-86248365 None:intergenic 30.0%
!! TTGTTTTTCCCTGATATTCA+TGG + chr7_2:86248527-86248546 None:intergenic 30.0%
AGGTTCATGTATACAAAAAG+AGG + chr7_2:86248553-86248572 None:intergenic 30.0%
TATAGATGCATGCAAATAAC+AGG + chr7_2:86248573-86248592 None:intergenic 30.0%
TCTTTGATTGAATGCATGTT+TGG - chr7_2:86248924-86248943 Msa1085480:intron 30.0%
!! TCACCACAATGATTTTGAAA+AGG - chr7_2:86248978-86248997 Msa1085480:intron 30.0%
! TAGCCTTTTCAAAATCATTG+TGG + chr7_2:86248984-86249003 None:intergenic 30.0%
!! AAGTGTTGTCAGATTTTCAA+AGG + chr7_2:86249017-86249036 None:intergenic 30.0%
TTTGAAAATCTGACAACACT+TGG - chr7_2:86249016-86249035 Msa1085480:intron 30.0%
TTGAAAATCTGACAACACTT+GGG - chr7_2:86249017-86249036 Msa1085480:intron 30.0%
TTGTGATGTCAATGTCATAT+TGG - chr7_2:86249045-86249064 Msa1085480:intron 30.0%
TGGGAAATTACATACATGTT+TGG + chr7_2:86249148-86249167 None:intergenic 30.0%
! CTTTATATCTAAAAGTGCGT+TGG + chr7_2:86249168-86249187 None:intergenic 30.0%
TTCAACATCTTATCGTTGAT+AGG + chr7_2:86249345-86249364 None:intergenic 30.0%
!!! TGTTGTTTTATGATTGTGAC+AGG - chr7_2:86249505-86249524 Msa1085480:intron 30.0%
TCTAAACAAATTGTGCTTAC+TGG - chr7_2:86249695-86249714 Msa1085480:CDS 30.0%
!! TTACTGGTTCAGTAAAACTA+TGG - chr7_2:86249711-86249730 Msa1085480:CDS 30.0%
AAAGTACAACCATGTTGTTA+AGG - chr7_2:86249808-86249827 Msa1085480:CDS 30.0%
!! GCTATTTTCTTCACATAATG+TGG - chr7_2:86249989-86250008 Msa1085480:five_prime_UTR 30.0%
TGATATATAGAGGTGGAAAT+TGG + chr7_2:86247941-86247960 None:intergenic 30.0%
ATGTAAATTAACCTGTTTGG+TGG + chr7_2:86248051-86248070 None:intergenic 30.0%
GCAATGTAAATTAACCTGTT+TGG + chr7_2:86248054-86248073 None:intergenic 30.0%
AAAAAACAAGGAGATCTTGA+GGG + chr7_2:86248345-86248364 None:intergenic 30.0%
AAAAAAACAAGGAGATCTTG+AGG + chr7_2:86248346-86248365 None:intergenic 30.0%
!! TTGTTTTTCCCTGATATTCA+TGG + chr7_2:86248527-86248546 None:intergenic 30.0%
AGGTTCATGTATACAAAAAG+AGG + chr7_2:86248553-86248572 None:intergenic 30.0%
TATAGATGCATGCAAATAAC+AGG + chr7_2:86248573-86248592 None:intergenic 30.0%
TCTTTGATTGAATGCATGTT+TGG - chr7_2:86248924-86248943 Msa1085480:intron 30.0%
!! TCACCACAATGATTTTGAAA+AGG - chr7_2:86248978-86248997 Msa1085480:intron 30.0%
! TAGCCTTTTCAAAATCATTG+TGG + chr7_2:86248984-86249003 None:intergenic 30.0%
!! AAGTGTTGTCAGATTTTCAA+AGG + chr7_2:86249017-86249036 None:intergenic 30.0%
TTTGAAAATCTGACAACACT+TGG - chr7_2:86249016-86249035 Msa1085480:intron 30.0%
TTGAAAATCTGACAACACTT+GGG - chr7_2:86249017-86249036 Msa1085480:intron 30.0%
TTGTGATGTCAATGTCATAT+TGG - chr7_2:86249045-86249064 Msa1085480:intron 30.0%
TGGGAAATTACATACATGTT+TGG + chr7_2:86249148-86249167 None:intergenic 30.0%
! CTTTATATCTAAAAGTGCGT+TGG + chr7_2:86249168-86249187 None:intergenic 30.0%
TTCAACATCTTATCGTTGAT+AGG + chr7_2:86249345-86249364 None:intergenic 30.0%
!!! TGTTGTTTTATGATTGTGAC+AGG - chr7_2:86249505-86249524 Msa1085480:intron 30.0%
TCTAAACAAATTGTGCTTAC+TGG - chr7_2:86249695-86249714 Msa1085480:CDS 30.0%
!! TTACTGGTTCAGTAAAACTA+TGG - chr7_2:86249711-86249730 Msa1085480:CDS 30.0%
AAAGTACAACCATGTTGTTA+AGG - chr7_2:86249808-86249827 Msa1085480:CDS 30.0%
!! GCTATTTTCTTCACATAATG+TGG - chr7_2:86249989-86250008 Msa1085480:five_prime_UTR 30.0%
TGGAATGTGATATATAGAGG+TGG + chr7_2:86247948-86247967 None:intergenic 35.0%
GGTTGGAATGTGATATATAG+AGG + chr7_2:86247951-86247970 None:intergenic 35.0%
! AGTTGTTACTGGTTATAAGG+AGG + chr7_2:86247981-86248000 None:intergenic 35.0%
! GTCAGTTGTTACTGGTTATA+AGG + chr7_2:86247984-86248003 None:intergenic 35.0%
! TTTTTCTCGTCAGTTGTTAC+TGG + chr7_2:86247992-86248011 None:intergenic 35.0%
ATAAGCTCCAACACAAAGAA+AGG - chr7_2:86248013-86248032 Msa1085480:exon 35.0%
ATTGCTTCCTTTCTTTGTGT+TGG + chr7_2:86248023-86248042 None:intergenic 35.0%
! TAAATTAACCTGTTTGGTGG+TGG + chr7_2:86248048-86248067 None:intergenic 35.0%
! CATGAAAAACTGAAGCATCT+TGG - chr7_2:86248163-86248182 Msa1085480:CDS 35.0%
! CATGCAAACAAAAGCATGAA+TGG + chr7_2:86248206-86248225 None:intergenic 35.0%
GACTATGCAAAACAAAACTC+AGG - chr7_2:86248238-86248257 Msa1085480:CDS 35.0%
!! GGCTTGAATTTGTTGTAAAC+AGG + chr7_2:86248319-86248338 None:intergenic 35.0%
! CCTTGTTGTCGAAAAAAACA+AGG + chr7_2:86248357-86248376 None:intergenic 35.0%
!!! CCTTGTTTTTTTCGACAACA+AGG - chr7_2:86248354-86248373 Msa1085480:CDS 35.0%
TTCTTATAAATGTGACCGGA+GGG + chr7_2:86248652-86248671 None:intergenic 35.0%
TTTCTTATAAATGTGACCGG+AGG + chr7_2:86248653-86248672 None:intergenic 35.0%
AACTTATTAGTTACTCCCTC+CGG + chr7_2:86248787-86248806 None:intergenic 35.0%
TGAATGCATGTTTGGTATCA+CGG - chr7_2:86248932-86248951 Msa1085480:intron 35.0%
ATTTGTTTGTCAAAACCACG+CGG + chr7_2:86249118-86249137 None:intergenic 35.0%
ATTCTCAATGAGTTAGGTGT+GGG + chr7_2:86249293-86249312 None:intergenic 35.0%
AATTCTCAATGAGTTAGGTG+TGG + chr7_2:86249294-86249313 None:intergenic 35.0%
ACCTAACTCATTGAGAATTG+AGG - chr7_2:86249295-86249314 Msa1085480:intron 35.0%
! TGTTTTATGATTGTGACAGG+TGG - chr7_2:86249508-86249527 Msa1085480:intron 35.0%
AGCATGCTCTTCAAATTTGT+TGG + chr7_2:86249543-86249562 None:intergenic 35.0%
GCTACTTGCTTTACAAAAGT+AGG + chr7_2:86249566-86249585 None:intergenic 35.0%
CAAGTAGCAAATCAAGCTAA+AGG - chr7_2:86249578-86249597 Msa1085480:intron 35.0%
CAGTAATGTCCTTAACAACA+TGG + chr7_2:86249820-86249839 None:intergenic 35.0%
CATGTTGTTAAGGACATTAC+TGG - chr7_2:86249818-86249837 Msa1085480:CDS 35.0%
TGTTAAGGACATTACTGGAA+AGG - chr7_2:86249823-86249842 Msa1085480:CDS 35.0%
GTTAAGGACATTACTGGAAA+GGG - chr7_2:86249824-86249843 Msa1085480:CDS 35.0%
TCCTATTGATGAAATAGCCA+AGG - chr7_2:86249877-86249896 Msa1085480:intron 35.0%
! ATAGCCAAGGCATTTAAACA+AGG - chr7_2:86249890-86249909 Msa1085480:intron 35.0%
! TTCTCCTTGTTTAAATGCCT+TGG + chr7_2:86249897-86249916 None:intergenic 35.0%
TGGAATGTGATATATAGAGG+TGG + chr7_2:86247948-86247967 None:intergenic 35.0%
GGTTGGAATGTGATATATAG+AGG + chr7_2:86247951-86247970 None:intergenic 35.0%
! AGTTGTTACTGGTTATAAGG+AGG + chr7_2:86247981-86248000 None:intergenic 35.0%
! GTCAGTTGTTACTGGTTATA+AGG + chr7_2:86247984-86248003 None:intergenic 35.0%
! TTTTTCTCGTCAGTTGTTAC+TGG + chr7_2:86247992-86248011 None:intergenic 35.0%
ATAAGCTCCAACACAAAGAA+AGG - chr7_2:86248013-86248032 Msa1085480:exon 35.0%
ATTGCTTCCTTTCTTTGTGT+TGG + chr7_2:86248023-86248042 None:intergenic 35.0%
! TAAATTAACCTGTTTGGTGG+TGG + chr7_2:86248048-86248067 None:intergenic 35.0%
! CATGAAAAACTGAAGCATCT+TGG - chr7_2:86248163-86248182 Msa1085480:CDS 35.0%
! CATGCAAACAAAAGCATGAA+TGG + chr7_2:86248206-86248225 None:intergenic 35.0%
GACTATGCAAAACAAAACTC+AGG - chr7_2:86248238-86248257 Msa1085480:CDS 35.0%
!! GGCTTGAATTTGTTGTAAAC+AGG + chr7_2:86248319-86248338 None:intergenic 35.0%
! CCTTGTTGTCGAAAAAAACA+AGG + chr7_2:86248357-86248376 None:intergenic 35.0%
!!! CCTTGTTTTTTTCGACAACA+AGG - chr7_2:86248354-86248373 Msa1085480:CDS 35.0%
TTCTTATAAATGTGACCGGA+GGG + chr7_2:86248652-86248671 None:intergenic 35.0%
TTTCTTATAAATGTGACCGG+AGG + chr7_2:86248653-86248672 None:intergenic 35.0%
AACTTATTAGTTACTCCCTC+CGG + chr7_2:86248787-86248806 None:intergenic 35.0%
TGAATGCATGTTTGGTATCA+CGG - chr7_2:86248932-86248951 Msa1085480:intron 35.0%
ATTTGTTTGTCAAAACCACG+CGG + chr7_2:86249118-86249137 None:intergenic 35.0%
ATTCTCAATGAGTTAGGTGT+GGG + chr7_2:86249293-86249312 None:intergenic 35.0%
AATTCTCAATGAGTTAGGTG+TGG + chr7_2:86249294-86249313 None:intergenic 35.0%
ACCTAACTCATTGAGAATTG+AGG - chr7_2:86249295-86249314 Msa1085480:intron 35.0%
! TGTTTTATGATTGTGACAGG+TGG - chr7_2:86249508-86249527 Msa1085480:intron 35.0%
AGCATGCTCTTCAAATTTGT+TGG + chr7_2:86249543-86249562 None:intergenic 35.0%
GCTACTTGCTTTACAAAAGT+AGG + chr7_2:86249566-86249585 None:intergenic 35.0%
CAAGTAGCAAATCAAGCTAA+AGG - chr7_2:86249578-86249597 Msa1085480:intron 35.0%
CAGTAATGTCCTTAACAACA+TGG + chr7_2:86249820-86249839 None:intergenic 35.0%
CATGTTGTTAAGGACATTAC+TGG - chr7_2:86249818-86249837 Msa1085480:CDS 35.0%
TGTTAAGGACATTACTGGAA+AGG - chr7_2:86249823-86249842 Msa1085480:CDS 35.0%
GTTAAGGACATTACTGGAAA+GGG - chr7_2:86249824-86249843 Msa1085480:CDS 35.0%
TCCTATTGATGAAATAGCCA+AGG - chr7_2:86249877-86249896 Msa1085480:intron 35.0%
! ATAGCCAAGGCATTTAAACA+AGG - chr7_2:86249890-86249909 Msa1085480:intron 35.0%
! TTCTCCTTGTTTAAATGCCT+TGG + chr7_2:86249897-86249916 None:intergenic 35.0%
TGGTTATAAGGAGGAAGACA+CGG + chr7_2:86247972-86247991 None:intergenic 40.0%
TACGTTCTGCCATGAATATC+AGG - chr7_2:86248515-86248534 Msa1085480:intron 40.0%
ACGTTCTGCCATGAATATCA+GGG - chr7_2:86248516-86248535 Msa1085480:intron 40.0%
ATAAGAACATGTACTCCCTC+CGG - chr7_2:86248634-86248653 Msa1085480:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr7_2:86248768-86248787 Msa1085480:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr7_2:86248769-86248788 Msa1085480:intron 40.0%
!! GATTTTCAAGGACAGCACTT+GGG + chr7_2:86249084-86249103 None:intergenic 40.0%
!! TGATTTTCAAGGACAGCACT+TGG + chr7_2:86249085-86249104 None:intergenic 40.0%
AGTGCTGTCCTTGAAAATCA+CGG - chr7_2:86249085-86249104 Msa1085480:intron 40.0%
GCCTCAATTCTCAATGAGTT+AGG + chr7_2:86249299-86249318 None:intergenic 40.0%
! CAAACTGCAGTTGGTACATA+TGG + chr7_2:86249411-86249430 None:intergenic 40.0%
ATGTACCAACTGCAGTTTGT+TGG - chr7_2:86249412-86249431 Msa1085480:intron 40.0%
TATCACCAACAAACTGCAGT+TGG + chr7_2:86249420-86249439 None:intergenic 40.0%
AGCTGAGAAAGTAGTGAATG+AGG - chr7_2:86249625-86249644 Msa1085480:CDS 40.0%
GGTTCAGTAAAACTATGGTC+AGG - chr7_2:86249716-86249735 Msa1085480:CDS 40.0%
! GCCTTGGCTATTTCATCAAT+AGG + chr7_2:86249881-86249900 None:intergenic 40.0%
TGTGGTTGAGAAAGATGTAG+AGG - chr7_2:86250007-86250026 Msa1085480:five_prime_UTR 40.0%
GTGGTTGAGAAAGATGTAGA+GGG - chr7_2:86250008-86250027 Msa1085480:five_prime_UTR 40.0%
TGGTTATAAGGAGGAAGACA+CGG + chr7_2:86247972-86247991 None:intergenic 40.0%
TACGTTCTGCCATGAATATC+AGG - chr7_2:86248515-86248534 Msa1085480:intron 40.0%
ACGTTCTGCCATGAATATCA+GGG - chr7_2:86248516-86248535 Msa1085480:intron 40.0%
ATAAGAACATGTACTCCCTC+CGG - chr7_2:86248634-86248653 Msa1085480:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr7_2:86248768-86248787 Msa1085480:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr7_2:86248769-86248788 Msa1085480:intron 40.0%
!! GATTTTCAAGGACAGCACTT+GGG + chr7_2:86249084-86249103 None:intergenic 40.0%
!! TGATTTTCAAGGACAGCACT+TGG + chr7_2:86249085-86249104 None:intergenic 40.0%
AGTGCTGTCCTTGAAAATCA+CGG - chr7_2:86249085-86249104 Msa1085480:intron 40.0%
GCCTCAATTCTCAATGAGTT+AGG + chr7_2:86249299-86249318 None:intergenic 40.0%
! CAAACTGCAGTTGGTACATA+TGG + chr7_2:86249411-86249430 None:intergenic 40.0%
ATGTACCAACTGCAGTTTGT+TGG - chr7_2:86249412-86249431 Msa1085480:intron 40.0%
TATCACCAACAAACTGCAGT+TGG + chr7_2:86249420-86249439 None:intergenic 40.0%
AGCTGAGAAAGTAGTGAATG+AGG - chr7_2:86249625-86249644 Msa1085480:CDS 40.0%
GGTTCAGTAAAACTATGGTC+AGG - chr7_2:86249716-86249735 Msa1085480:CDS 40.0%
! GCCTTGGCTATTTCATCAAT+AGG + chr7_2:86249881-86249900 None:intergenic 40.0%
TGTGGTTGAGAAAGATGTAG+AGG - chr7_2:86250007-86250026 Msa1085480:five_prime_UTR 40.0%
GTGGTTGAGAAAGATGTAGA+GGG - chr7_2:86250008-86250027 Msa1085480:five_prime_UTR 40.0%
TATAAGGAGGAAGACACGGT+TGG + chr7_2:86247968-86247987 None:intergenic 45.0%
GTTCCAACAGCAGATCTCAA+AGG + chr7_2:86248265-86248284 None:intergenic 45.0%
GGTAGTGACAGTACCTTCAA+CGG + chr7_2:86248287-86248306 None:intergenic 45.0%
GTTGTAAACAGGACCGAGAA+CGG + chr7_2:86248308-86248327 None:intergenic 45.0%
TGCATGCATGTCAAGTTTCG+AGG + chr7_2:86248829-86248848 None:intergenic 45.0%
AGCACTTGGGTGACATTGTA+GGG + chr7_2:86249071-86249090 None:intergenic 45.0%
! GTGAGTCACCGTGATTTTCA+AGG + chr7_2:86249096-86249115 None:intergenic 45.0%
TCAATGAGTTAGGTGTGGGA+TGG + chr7_2:86249289-86249308 None:intergenic 45.0%
GTTCACATGCGATGTGACTT+GGG + chr7_2:86249374-86249393 None:intergenic 45.0%
CAAGTCACATCGCATGTGAA+CGG - chr7_2:86249373-86249392 Msa1085480:intron 45.0%
! CTGCAGTTGGTACATATGGT+TGG + chr7_2:86249407-86249426 None:intergenic 45.0%
GTAGTGAATGAGGCTCAATC+TGG - chr7_2:86249635-86249654 Msa1085480:CDS 45.0%
TGAATGAGGCTCAATCTGGT+GGG - chr7_2:86249639-86249658 Msa1085480:CDS 45.0%
GGAAAGGGATACACACTGTT+TGG - chr7_2:86249839-86249858 Msa1085480:intron 45.0%
! GAAAGATGTAGAGGGTGTGT+AGG - chr7_2:86250016-86250035 Msa1085480:five_prime_UTR 45.0%
! AGAGGGTGTGTAGGTATGAA+TGG - chr7_2:86250025-86250044 Msa1085480:five_prime_UTR 45.0%
TATAAGGAGGAAGACACGGT+TGG + chr7_2:86247968-86247987 None:intergenic 45.0%
GTTCCAACAGCAGATCTCAA+AGG + chr7_2:86248265-86248284 None:intergenic 45.0%
GGTAGTGACAGTACCTTCAA+CGG + chr7_2:86248287-86248306 None:intergenic 45.0%
GTTGTAAACAGGACCGAGAA+CGG + chr7_2:86248308-86248327 None:intergenic 45.0%
TGCATGCATGTCAAGTTTCG+AGG + chr7_2:86248829-86248848 None:intergenic 45.0%
AGCACTTGGGTGACATTGTA+GGG + chr7_2:86249071-86249090 None:intergenic 45.0%
! GTGAGTCACCGTGATTTTCA+AGG + chr7_2:86249096-86249115 None:intergenic 45.0%
TCAATGAGTTAGGTGTGGGA+TGG + chr7_2:86249289-86249308 None:intergenic 45.0%
GTTCACATGCGATGTGACTT+GGG + chr7_2:86249374-86249393 None:intergenic 45.0%
CAAGTCACATCGCATGTGAA+CGG - chr7_2:86249373-86249392 Msa1085480:intron 45.0%
! CTGCAGTTGGTACATATGGT+TGG + chr7_2:86249407-86249426 None:intergenic 45.0%
GTAGTGAATGAGGCTCAATC+TGG - chr7_2:86249635-86249654 Msa1085480:CDS 45.0%
TGAATGAGGCTCAATCTGGT+GGG - chr7_2:86249639-86249658 Msa1085480:CDS 45.0%
GGAAAGGGATACACACTGTT+TGG - chr7_2:86249839-86249858 Msa1085480:intron 45.0%
! GAAAGATGTAGAGGGTGTGT+AGG - chr7_2:86250016-86250035 Msa1085480:five_prime_UTR 45.0%
! AGAGGGTGTGTAGGTATGAA+TGG - chr7_2:86250025-86250044 Msa1085480:five_prime_UTR 45.0%
AGCAATGACCACCACCAAAC+AGG - chr7_2:86248037-86248056 Msa1085480:CDS 50.0%
!! GGACCTTTGAGATCTGCTGT+TGG - chr7_2:86248259-86248278 Msa1085480:CDS 50.0%
TCTGCTGTTGGAACCGTTGA+AGG - chr7_2:86248271-86248290 Msa1085480:CDS 50.0%
GGTACTGTCACTACCGTTCT+CGG - chr7_2:86248292-86248311 Msa1085480:CDS 50.0%
ACAAGGAGATCTTGAGGGAG+AGG + chr7_2:86248340-86248359 None:intergenic 50.0%
CAGCACTTGGGTGACATTGT+AGG + chr7_2:86249072-86249091 None:intergenic 50.0%
TGAGTTAGGTGTGGGATGGT+AGG + chr7_2:86249285-86249304 None:intergenic 50.0%
CGTTCACATGCGATGTGACT+TGG + chr7_2:86249375-86249394 None:intergenic 50.0%
GTGAATGAGGCTCAATCTGG+TGG - chr7_2:86249638-86249657 Msa1085480:CDS 50.0%
GAATGAGGCTCAATCTGGTG+GGG - chr7_2:86249640-86249659 Msa1085480:CDS 50.0%
!! CTGCATGGAATGGTGGATAG+TGG + chr7_2:86249748-86249767 None:intergenic 50.0%
CTATCCACCATTCCATGCAG+TGG - chr7_2:86249748-86249767 Msa1085480:CDS 50.0%
ATCTCTGCCACTGCATGGAA+TGG + chr7_2:86249758-86249777 None:intergenic 50.0%
ATTCCATGCAGTGGCAGAGA+TGG - chr7_2:86249757-86249776 Msa1085480:CDS 50.0%
CAGTTCCAACAGCTGCACAT+TGG - chr7_2:86249780-86249799 Msa1085480:CDS 50.0%
TCTGACCAATGTGCAGCTGT+TGG + chr7_2:86249788-86249807 None:intergenic 50.0%
AGCAATGACCACCACCAAAC+AGG - chr7_2:86248037-86248056 Msa1085480:CDS 50.0%
!! GGACCTTTGAGATCTGCTGT+TGG - chr7_2:86248259-86248278 Msa1085480:CDS 50.0%
TCTGCTGTTGGAACCGTTGA+AGG - chr7_2:86248271-86248290 Msa1085480:CDS 50.0%
GGTACTGTCACTACCGTTCT+CGG - chr7_2:86248292-86248311 Msa1085480:CDS 50.0%
ACAAGGAGATCTTGAGGGAG+AGG + chr7_2:86248340-86248359 None:intergenic 50.0%
CAGCACTTGGGTGACATTGT+AGG + chr7_2:86249072-86249091 None:intergenic 50.0%
TGAGTTAGGTGTGGGATGGT+AGG + chr7_2:86249285-86249304 None:intergenic 50.0%
CGTTCACATGCGATGTGACT+TGG + chr7_2:86249375-86249394 None:intergenic 50.0%
GTGAATGAGGCTCAATCTGG+TGG - chr7_2:86249638-86249657 Msa1085480:CDS 50.0%
GAATGAGGCTCAATCTGGTG+GGG - chr7_2:86249640-86249659 Msa1085480:CDS 50.0%
!! CTGCATGGAATGGTGGATAG+TGG + chr7_2:86249748-86249767 None:intergenic 50.0%
CTATCCACCATTCCATGCAG+TGG - chr7_2:86249748-86249767 Msa1085480:CDS 50.0%
ATCTCTGCCACTGCATGGAA+TGG + chr7_2:86249758-86249777 None:intergenic 50.0%
ATTCCATGCAGTGGCAGAGA+TGG - chr7_2:86249757-86249776 Msa1085480:CDS 50.0%
CAGTTCCAACAGCTGCACAT+TGG - chr7_2:86249780-86249799 Msa1085480:CDS 50.0%
TCTGACCAATGTGCAGCTGT+TGG + chr7_2:86249788-86249807 None:intergenic 50.0%
TCTGCCACTGCATGGAATGG+TGG + chr7_2:86249755-86249774 None:intergenic 55.0%
TCTGCCACTGCATGGAATGG+TGG + chr7_2:86249755-86249774 None:intergenic 55.0%
AATCACGGTGACTCACCGCG+TGG - chr7_2:86249100-86249119 Msa1085480:intron 60.0%
CTGCCATCTCTGCCACTGCA+TGG + chr7_2:86249763-86249782 None:intergenic 60.0%
AATCACGGTGACTCACCGCG+TGG - chr7_2:86249100-86249119 Msa1085480:intron 60.0%
CTGCCATCTCTGCCACTGCA+TGG + chr7_2:86249763-86249782 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 86247835 86250160 86247835 ID=Msa1085480;Name=Msa1085480
chr7_2 mRNA 86247835 86250160 86247835 ID=Msa1085480-mRNA-1;Parent=Msa1085480;Name=Msa1085480-mRNA-1;_AED=0.02;_eAED=0.02;_QI=206|1|1|1|1|1|3|190|231
chr7_2 exon 86247835 86248468 86247835 ID=Msa1085480-mRNA-1:exon:23327;Parent=Msa1085480-mRNA-1
chr7_2 exon 86249620 86249853 86249620 ID=Msa1085480-mRNA-1:exon:23326;Parent=Msa1085480-mRNA-1
chr7_2 exon 86249937 86250160 86249937 ID=Msa1085480-mRNA-1:exon:23325;Parent=Msa1085480-mRNA-1
chr7_2 five_prime_UTR 86249955 86250160 86249955 ID=Msa1085480-mRNA-1:five_prime_utr;Parent=Msa1085480-mRNA-1
chr7_2 CDS 86249937 86249954 86249937 ID=Msa1085480-mRNA-1:cds;Parent=Msa1085480-mRNA-1
chr7_2 CDS 86249620 86249853 86249620 ID=Msa1085480-mRNA-1:cds;Parent=Msa1085480-mRNA-1
chr7_2 CDS 86248025 86248468 86248025 ID=Msa1085480-mRNA-1:cds;Parent=Msa1085480-mRNA-1
chr7_2 three_prime_UTR 86247835 86248024 86247835 ID=Msa1085480-mRNA-1:three_prime_utr;Parent=Msa1085480-mRNA-1
Gene Sequence

>Msa1085480

ATGACCACCACCAAACAGATGGATGTAGAGAGAAATGATCATGAAAAACTGAAGCATCTTGGATTTGTGAGAATTGCTGCCATTCATGCTTTTGTTTGCATGTCGAGTCTGTATGACTATGCAAAACAAAACTCAGGACCTTTGAGATCTGCTGTTGGAACCGTTGAAGGTACTGTCACTACCGTTCTCGGTCCTGTTTACAACAAATTCAAGCCTCTCCCTCAAGATCTCCTTGTTTTTTTCGACAACAAGGTGGATGAAGCAACCAACAAATTTGAAGAGCATGCTCCTACTTTTGTAAAGCAAGTAGCAAATCAAGCTAAAGGTTTGATTCAAGAAGCGACACACAAAGCTGAGAAAGTAGTGAATGAGGCTCAATCTGGTGGGGCAAAAGCAGCTGCAAATTATGTAGCTACAGAATCTAAACAAATTGTGCTTACTGGTTCAGTAAAACTATGGTCAGGTATCAACCACTATCCACCATTCCATGCAGTGGCAGAGATGGCAGTTCCAACAGCTGCACATTGGTCAGAAAAGTACAACCATGTTGTTAAGGACATTACTGGAAAGGGATACACACTGTTTGGATATTTGCCTTTGATTCCTATTGATGAAATAGCCAAGGCATTTAAACAAGGAGAAGTTAATGTGAGTGCAGATCATGAAACATCTTCTGTTGAAGATTTTTCTGATTGA
Protein sequence

>Msa1085480

MTTTKQMDVERNDHEKLKHLGFVRIAAIHAFVCMSSLYDYAKQNSGPLRSAVGTVEGTVTTVLGPVYNKFKPLPQDLLVFFDNKVDEATNKFEEHAPTFVKQVANQAKGLIQEATHKAEKVVNEAQSGGAKAAANYVATESKQIVLTGSVKLWSGINHYPPFHAVAEMAVPTAAHWSEKYNHVVKDITGKGYTLFGYLPLIPIDEIAKAFKQGEVNVSADHETSSVEDFSD*