AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0661120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661120 MtrunA17_Chr4g0032181 94.515 237 13 0 31 267 17 253 1.97e-167 463
Msa0661120 MtrunA17_Chr3g0112261 61.004 259 101 0 3 261 7 265 3.06e-121 347
Msa0661120 MtrunA17_Chr3g0112271 62.698 252 94 0 11 262 13 264 2.42e-119 342
Msa0661120 MtrunA17_Chr3g0112151 59.160 262 107 0 1 262 72 333 1.60e-117 340
Msa0661120 MtrunA17_Chr4g0032141 62.451 253 95 0 10 262 51 303 1.22e-115 334
Msa0661120 MtrunA17_Chr3g0112171 59.542 262 106 0 1 262 1 262 3.13e-113 327
Msa0661120 MtrunA17_Chr3g0112141 59.677 248 100 0 15 262 17 264 9.90e-111 320
Msa0661120 MtrunA17_Chr7g0239511 55.805 267 111 3 1 261 1 266 2.66e-105 306
Msa0661120 MtrunA17_Chr7g0239451 55.094 265 114 2 1 260 1 265 2.17e-104 304
Msa0661120 MtrunA17_Chr7g0239481 53.962 265 118 2 1 261 1 265 7.99e-102 297
Msa0661120 MtrunA17_Chr7g0239501 54.135 266 117 3 1 261 1 266 2.84e-101 296
Msa0661120 MtrunA17_Chr7g0239491 53.232 263 120 1 1 260 1 263 1.04e-98 289
Msa0661120 MtrunA17_Chr7g0239461 49.242 264 81 4 1 260 1 215 1.27e-82 247
Msa0661120 MtrunA17_Chr4g0075531 32.927 246 160 4 15 259 7 248 2.03e-33 122
Msa0661120 MtrunA17_Chr3g0112321 55.102 98 28 2 148 242 3 87 1.93e-29 106
Msa0661120 MtrunA17_Chr3g0122901 33.333 246 153 7 21 261 81 320 7.16e-29 111
Msa0661120 MtrunA17_Chr7g0259521 32.400 250 161 5 15 258 6 253 1.08e-26 104
Msa0661120 MtrunA17_Chr8g0357481 30.888 259 158 6 15 259 14 265 7.19e-26 103
Msa0661120 MtrunA17_Chr4g0074511 29.434 265 175 6 8 263 33 294 3.38e-25 101
Msa0661120 MtrunA17_Chr7g0259531 29.880 251 168 4 15 259 11 259 7.95e-25 99.8
Msa0661120 MtrunA17_Chr0c30g0494101 29.323 266 172 7 8 263 33 292 1.89e-23 96.7
Msa0661120 MtrunA17_Chr4g0026001 31.518 257 155 11 15 261 14 259 6.10e-23 94.7
Msa0661120 MtrunA17_Chr4g0044111 30.469 256 162 8 15 261 13 261 2.05e-22 93.6
Msa0661120 MtrunA17_Chr7g0266981 29.688 256 164 8 15 261 13 261 6.72e-22 92.0
Msa0661120 MtrunA17_Chr4g0028921 28.346 254 175 5 15 261 11 264 9.75e-22 91.7
Msa0661120 MtrunA17_Chr4g0028911 27.953 254 176 5 15 261 11 264 3.25e-21 90.1
Msa0661120 MtrunA17_Chr4g0025981 30.112 269 141 12 16 261 42 286 1.44e-20 89.4
Msa0661120 MtrunA17_Chr7g0272961 29.572 257 170 6 15 263 40 293 2.48e-20 88.2
Msa0661120 MtrunA17_Chr6g0459301 28.195 266 180 7 1 259 1 262 3.15e-20 87.4
Msa0661120 MtrunA17_Chr3g0084871 30.769 273 164 10 5 260 6 270 3.84e-20 87.4
Msa0661120 MtrunA17_Chr1g0182511 31.890 254 158 7 15 258 14 262 3.97e-20 87.0
Msa0661120 MtrunA17_Chr7g0266891 28.906 256 166 8 15 261 7 255 7.03e-20 86.3
Msa0661120 MtrunA17_Chr7g0244061 27.491 291 173 8 6 266 20 302 2.27e-19 85.5
Msa0661120 MtrunA17_Chr6g0459281 28.679 265 175 7 1 257 1 259 2.89e-19 84.7
Msa0661120 MtrunA17_Chr6g0459261 28.571 266 178 7 1 259 13 273 3.75e-19 84.7
Msa0661120 MtrunA17_Chr1g0191751 28.794 257 170 8 15 262 101 353 1.46e-18 84.0
Msa0661120 MtrunA17_Chr1g0171831 26.295 251 169 8 15 251 14 262 5.83e-18 81.3
Msa0661120 MtrunA17_Chr2g0324021 28.195 266 171 9 11 264 2 259 6.54e-18 80.9
Msa0661120 MtrunA17_Chr6g0459271 28.947 266 177 7 1 259 1 261 6.74e-18 80.9
Msa0661120 MtrunA17_Chr4g0025971 26.641 259 175 6 10 261 9 259 8.04e-18 80.9
Msa0661120 MtrunA17_Chr4g0040371 28.214 280 161 12 5 260 8 271 1.17e-17 80.5
Msa0661120 MtrunA17_Chr6g0459351 27.273 264 180 7 1 257 1 259 2.39e-17 79.7
Msa0661120 MtrunA17_Chr7g0252031 27.376 263 164 6 15 260 35 287 7.36e-17 78.6
Msa0661120 MtrunA17_Chr6g0459331 28.571 266 178 7 1 259 1 261 3.90e-16 76.3
Msa0661120 MtrunA17_Chr4g0044131 25.573 262 178 8 15 265 8 263 5.84e-16 75.9
Msa0661120 MtrunA17_Chr7g0249571 28.646 192 119 4 22 202 45 229 2.65e-15 74.3
Msa0661120 MtrunA17_Chr7g0270441 26.641 259 177 8 11 261 2 255 3.03e-15 73.6
Msa0661120 MtrunA17_Chr7g0268591 25.283 265 180 6 8 260 26 284 6.66e-15 73.2
Msa0661120 MtrunA17_Chr6g0466681 30.435 253 148 10 15 250 64 305 5.15e-14 70.9
Msa0661120 MtrunA17_Chr7g0266991 30.288 208 110 7 13 209 18 201 6.04e-13 66.6
Msa0661120 MtrunA17_Chr7g0270451 28.846 260 167 10 12 261 3 254 1.35e-12 66.2
Msa0661120 MtrunA17_Chr7g0267011 26.596 188 133 4 15 200 8 192 1.91e-12 64.7
Msa0661120 MtrunA17_Chr4g0038121 30.526 190 121 7 76 259 186 370 4.14e-12 65.5
Msa0661120 MtrunA17_Chr7g0267001 24.231 260 166 8 15 265 8 245 1.42e-11 63.2
Msa0661120 MtrunA17_Chr7g0234681 31.148 183 117 7 82 259 192 370 2.48e-11 63.2
Msa0661120 MtrunA17_Chr4g0032171 93.333 30 2 0 1 30 4 33 2.95e-11 57.4
Msa0661120 MtrunA17_Chr4g0044121 28.488 172 110 5 99 261 1 168 3.62e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661120 AT1G07440.1 63.602 261 94 1 7 267 4 263 1.09e-122 350
Msa0661120 AT2G29150.1 64.259 263 92 2 1 262 1 262 8.83e-120 343
Msa0661120 AT5G06060.1 61.265 253 98 0 10 262 4 256 3.63e-119 342
Msa0661120 AT2G29350.1 64.122 262 93 1 1 262 1 261 1.98e-118 340
Msa0661120 AT2G29340.2 61.240 258 100 0 10 267 2 259 2.11e-118 340
Msa0661120 AT2G29340.1 61.240 258 100 0 10 267 2 259 8.95e-118 340
Msa0661120 AT2G29290.2 63.178 258 95 0 10 267 2 259 1.65e-117 337
Msa0661120 AT2G29360.1 64.259 263 93 1 1 262 1 263 1.66e-117 338
Msa0661120 AT2G30670.1 61.660 253 97 0 10 262 2 254 9.23e-116 333
Msa0661120 AT2G29310.1 60.000 255 102 0 10 264 2 256 3.41e-112 324
Msa0661120 AT2G29370.1 61.597 263 100 1 1 262 1 263 2.54e-111 322
Msa0661120 AT1G07450.1 63.347 251 91 1 12 262 5 254 1.86e-110 319
Msa0661120 AT2G29260.1 59.363 251 102 0 12 262 65 315 6.16e-110 320
Msa0661120 AT2G29330.1 57.752 258 109 0 10 267 2 259 3.09e-109 316
Msa0661120 AT2G29320.1 58.203 256 106 1 13 267 11 266 1.48e-107 312
Msa0661120 AT2G29300.1 57.708 253 106 1 10 261 2 254 3.69e-106 308
Msa0661120 AT2G29290.1 63.913 230 83 0 33 262 8 237 4.31e-105 305
Msa0661120 AT2G29300.2 57.540 252 106 1 10 260 2 253 7.96e-105 306
Msa0661120 AT2G29350.3 64.126 223 79 1 40 262 2 223 4.85e-101 294
Msa0661120 AT2G29310.2 59.914 232 93 0 31 262 21 252 3.92e-99 291
Msa0661120 AT2G29320.2 56.723 238 102 1 31 267 11 248 6.25e-96 282
Msa0661120 AT2G29350.2 64.423 208 74 0 1 208 1 208 2.46e-92 272
Msa0661120 AT2G29340.3 58.000 200 84 0 10 209 2 201 1.84e-85 254
Msa0661120 AT2G29340.4 58.000 200 84 0 10 209 2 201 1.84e-85 254
Msa0661120 AT2G29310.3 56.500 200 87 0 10 209 2 201 4.22e-81 243
Msa0661120 AT2G29320.3 56.545 191 82 1 13 202 11 201 1.26e-75 229
Msa0661120 AT1G07440.2 60.843 166 65 0 7 172 4 169 1.43e-71 218
Msa0661120 AT2G29310.4 55.307 179 80 0 31 209 21 199 4.59e-68 209
Msa0661120 AT2G29170.1 62.745 102 37 1 1 101 1 102 1.45e-38 131
Msa0661120 AT4G05530.1 31.707 246 163 4 15 259 9 250 2.70e-30 114
Msa0661120 AT3G03980.1 31.852 270 163 7 5 258 4 268 7.87e-29 110
Msa0661120 AT3G55310.1 30.078 256 168 7 15 261 17 270 4.53e-27 106
Msa0661120 AT1G24360.1 32.377 244 158 6 21 261 80 319 1.12e-26 106
Msa0661120 AT4G13180.1 31.061 264 172 7 5 261 3 263 1.16e-26 105
Msa0661120 AT1G63380.3 30.739 257 166 7 15 261 15 269 8.90e-26 102
Msa0661120 AT1G63380.1 30.739 257 166 7 15 261 17 271 8.95e-26 103
Msa0661120 AT1G63380.4 30.739 257 166 7 15 261 19 273 1.13e-25 102
Msa0661120 AT3G55290.2 29.297 256 170 7 15 261 17 270 1.41e-25 102
Msa0661120 AT3G04000.1 31.321 265 162 8 15 265 14 272 1.41e-25 102
Msa0661120 AT1G63380.2 30.739 257 166 7 15 261 22 276 1.42e-25 102
Msa0661120 AT3G55290.1 29.297 256 170 7 15 261 18 271 1.57e-25 102
Msa0661120 AT1G54870.1 32.443 262 154 10 15 262 82 334 9.04e-25 101
Msa0661120 AT5G18210.1 30.000 260 161 7 14 259 7 259 9.95e-25 99.8
Msa0661120 AT1G54870.2 32.443 262 154 10 15 262 84 336 1.13e-24 101
Msa0661120 AT3G05260.1 32.707 266 165 8 7 262 27 288 1.79e-24 99.8
Msa0661120 AT3G12800.1 28.346 254 172 4 15 259 10 262 2.05e-24 99.8
Msa0661120 AT1G62610.2 29.961 257 168 7 15 261 13 267 2.81e-24 99.0
Msa0661120 AT1G62610.4 29.961 257 168 7 15 261 19 273 3.00e-24 99.0
Msa0661120 AT1G62610.3 29.961 257 168 7 15 261 15 269 3.03e-24 99.0
Msa0661120 AT1G62610.1 29.961 257 168 7 15 261 14 268 3.07e-24 99.0
Msa0661120 AT5G18210.2 30.165 242 148 7 14 241 7 241 1.11e-21 92.0
Msa0661120 AT2G17845.1 27.734 256 171 7 15 258 47 300 5.56e-21 90.5
Msa0661120 AT3G46170.1 27.344 256 175 7 15 261 26 279 6.62e-21 90.1
Msa0661120 AT2G47120.1 28.125 256 174 9 11 261 2 252 6.02e-20 86.7
Msa0661120 AT2G47120.2 28.175 252 171 9 15 261 7 253 9.39e-20 86.3
Msa0661120 AT3G51680.1 27.586 290 180 11 1 266 20 303 1.96e-18 83.6
Msa0661120 AT3G42960.1 29.588 267 164 10 10 260 3 261 1.22e-17 80.9
Msa0661120 AT3G26760.1 28.044 271 170 9 5 260 30 290 2.25e-17 80.5
Msa0661120 AT4G03140.1 26.172 256 175 6 15 260 78 329 3.64e-17 80.5
Msa0661120 AT1G52340.1 27.839 273 155 8 15 260 18 275 2.02e-15 74.7
Msa0661120 AT2G47130.1 27.308 260 172 9 11 261 2 253 2.51e-15 73.9
Msa0661120 AT3G29260.1 27.757 263 167 8 11 261 2 253 3.47e-15 73.6
Msa0661120 AT3G06060.1 28.500 200 132 5 9 203 31 224 2.71e-14 72.0
Msa0661120 AT3G06060.2 28.500 200 132 5 9 203 48 241 4.16e-14 71.6
Msa0661120 AT3G26770.1 27.273 264 163 8 15 260 41 293 8.85e-14 70.5
Msa0661120 AT5G19200.1 26.852 216 149 4 9 220 29 239 1.04e-12 67.4
Msa0661120 AT3G03330.1 26.531 196 135 5 14 202 40 233 2.17e-12 66.6
Msa0661120 AT1G10310.1 31.016 187 125 4 19 204 19 202 6.46e-12 64.3
Msa0661120 AT5G65205.1 26.596 188 127 5 21 205 12 191 3.18e-11 62.8
Msa0661120 AT3G29250.2 26.894 264 177 9 11 265 2 258 4.70e-11 62.0
Msa0661120 AT3G29250.3 26.923 260 174 9 15 265 42 294 8.06e-11 61.6
Msa0661120 AT3G29250.1 26.923 260 174 9 15 265 44 296 9.68e-11 61.6

Find 83 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCAAGATTTCTCATTTC+TGG 0.136218 4_3:-72313500 Msa0661120:CDS
TGATTCTACCTTCTGGTCTA+AGG 0.215896 4_3:+72314454 None:intergenic
TAAAATGAAAACACGACTTA+TGG 0.217681 4_3:+72312437 None:intergenic
AAGCATGTGTATTTGTAAAA+AGG 0.219514 4_3:-72312130 Msa0661120:three_prime_UTR
CAAATACACATGCTTTAATA+TGG 0.241647 4_3:+72312138 None:intergenic
GGTAGAATCACCAAGTATTA+AGG 0.311024 4_3:-72314441 Msa0661120:five_prime_UTR
CAAGCCTAGCTTATTGTAGA+AGG 0.334137 4_3:+72312544 None:intergenic
GTAAAATCTAGTGATTGTTT+TGG 0.345117 4_3:+72313523 None:intergenic
CCTGCAGCCTCTTTCATAAC+AGG 0.358569 4_3:-72312609 Msa0661120:CDS
AAAATGAAAACACGACTTAT+GGG 0.374397 4_3:+72312438 None:intergenic
TACTTGGTGATTCTACCTTC+TGG 0.374722 4_3:+72314447 None:intergenic
GGACAGACCATTTGTATTGA+TGG 0.378202 4_3:-72312588 Msa0661120:CDS
TGCCATTTCTCCTTAATACT+TGG 0.386036 4_3:+72314431 None:intergenic
ACTGGTGGATCCAAAGGAAT+AGG 0.388939 4_3:-72314348 Msa0661120:CDS
AGAACCCCTCTTGGACGTAT+TGG 0.397142 4_3:-72312675 Msa0661120:CDS
AGATAGCAACCCTATTCCTT+TGG 0.397711 4_3:+72314338 None:intergenic
TTCATTTCAAGAACCCCTCT+TGG 0.404796 4_3:-72312684 Msa0661120:CDS
TCAATGAATGCTTACATCAA+TGG 0.405589 4_3:-72313802 Msa0661120:CDS
AGAAGAAATGTCTACAAATC+TGG 0.410397 4_3:+72312200 None:intergenic
CACCAAGTATTAAGGAGAAA+TGG 0.414952 4_3:-72314433 Msa0661120:exon
ACAAGTATCGAATACGTATC+TGG 0.415745 4_3:+72312094 None:intergenic
GATTCTACCTTCTGGTCTAA+GGG 0.431428 4_3:+72314455 None:intergenic
GCAGCAAGAGGGGTTCTGAT+TGG 0.447876 4_3:+72313147 None:intergenic
CAATGAATGCTTACATCAAT+GGG 0.449372 4_3:-72313801 Msa0661120:CDS
ACCAAGGAAGACACCTCTTC+TGG 0.466532 4_3:+72312648 None:intergenic
AACACATGAAAACTTCTACT+TGG 0.466597 4_3:-72314530 Msa0661120:five_prime_UTR
GAGTTTGAAGCTTTGAGTAA+TGG 0.471691 4_3:+72313430 None:intergenic
TATGACTAGGTATGATATAG+TGG 0.472016 4_3:-72313882 Msa0661120:intron
AGAGGGGTTCTGATTGGCCC+TGG 0.476277 4_3:+72313153 None:intergenic
AAATAGTAAGTGGTCTTTGA+AGG 0.483818 4_3:-72314388 Msa0661120:CDS
TTATTTGTGAGAGTTTCATA+TGG 0.485228 4_3:-72312235 Msa0661120:three_prime_UTR
ACCAGAAGAGGTGTCTTCCT+TGG 0.491068 4_3:-72312649 Msa0661120:CDS
TGGAGCAATTAGCAGAGCTA+GGG 0.491202 4_3:-72313862 Msa0661120:CDS
ATGACAGCTCTGGTCACTGG+TGG 0.504668 4_3:-72314363 Msa0661120:CDS
ACCATCTATAGTGCAGCAAA+AGG 0.506661 4_3:-72313352 Msa0661120:intron
TAACCCTCCATCAATACAAA+TGG 0.507511 4_3:+72312581 None:intergenic
TATTTGTGAGAGTTTCATAT+GGG 0.511753 4_3:-72312234 Msa0661120:three_prime_UTR
TTTGAAGGGGATGACAGCTC+TGG 0.513224 4_3:-72314373 Msa0661120:CDS
GGGATGACAGCTCTGGTCAC+TGG 0.516153 4_3:-72314366 Msa0661120:CDS
TGAGTAATGGATGTGCAAGC+TGG 0.522910 4_3:+72313443 None:intergenic
AATAGTAAGTGGTCTTTGAA+GGG 0.525324 4_3:-72314387 Msa0661120:CDS
GTGGAGCAATTAGCAGAGCT+AGG 0.526426 4_3:-72313863 Msa0661120:CDS
AGGACTAATTGTGTTGCACC+AGG 0.529569 4_3:-72313171 Msa0661120:CDS
TTACATCAATGGGTCACAAA+AGG 0.532043 4_3:-72313791 Msa0661120:CDS
CAAGTAGCAGAAATAGTAAG+TGG 0.538795 4_3:-72314398 Msa0661120:CDS
CTGGTCACTGGTGGATCCAA+AGG 0.539538 4_3:-72314354 Msa0661120:CDS
AGAGTCTCTTCTGAGTTCAA+TGG 0.543714 4_3:-72313704 Msa0661120:CDS
ACAAAAGGATACAAAATCAC+TGG 0.547408 4_3:-72313776 Msa0661120:CDS
CAGACCATTTGTATTGATGG+AGG 0.547706 4_3:-72312585 Msa0661120:CDS
CCTGTTATGAAAGAGGCTGC+AGG 0.552564 4_3:+72312609 None:intergenic
GGGGTAGCATCACTAAATGT+AGG 0.552808 4_3:-72313376 Msa0661120:CDS
CTGGTGGATCCAAAGGAATA+GGG 0.554228 4_3:-72314347 Msa0661120:intron
CCACTGTACACACTTGTGCT+AGG 0.562221 4_3:-72313838 Msa0661120:CDS
AGACCATTTGTATTGATGGA+GGG 0.569442 4_3:-72312584 Msa0661120:CDS
TTCTACCTTCTGGTCTAAGG+GGG 0.569545 4_3:+72314457 None:intergenic
TTTCATGTCCTCCATTGGTG+GGG 0.574535 4_3:-72313395 Msa0661120:CDS
ATTCTACCTTCTGGTCTAAG+GGG 0.575493 4_3:+72314456 None:intergenic
GAAATTTAGGTGAACAATGT+GGG 0.576001 4_3:-72313556 Msa0661120:intron
AAATTACTTCTGCAGCAAGA+GGG 0.582091 4_3:+72313136 None:intergenic
GTCTCCTTCTACAATAAGCT+AGG 0.587750 4_3:-72312548 Msa0661120:exon
TAGTGATGCTACCCCACCAA+TGG 0.589871 4_3:+72313384 None:intergenic
AGTGGGCTAGAGACAACATA+AGG 0.591685 4_3:-72313191 Msa0661120:CDS
GTTCTCCAATACGTCCAAGA+GGG 0.595929 4_3:+72312670 None:intergenic
AAAATTACTTCTGCAGCAAG+AGG 0.596709 4_3:+72313135 None:intergenic
AGAAGAGGTGTCTTCCTTGG+TGG 0.599777 4_3:-72312646 Msa0661120:CDS
AAGAGGCTGCAGGTAAGCAT+AGG 0.600954 4_3:+72312619 None:intergenic
TGAAATTTAGGTGAACAATG+TGG 0.603162 4_3:-72313557 Msa0661120:intron
GTCATCTCGTGCACAAAGAG+AGG 0.604943 4_3:-72313738 Msa0661120:CDS
AATTACTTCTGCAGCAAGAG+GGG 0.609401 4_3:+72313137 None:intergenic
ATAGTAAGTGGTCTTTGAAG+GGG 0.610704 4_3:-72314386 Msa0661120:CDS
GGTCTGTCCTGTTATGAAAG+AGG 0.618109 4_3:+72312602 None:intergenic
TGATGCTACCCCACCAATGG+AGG 0.621694 4_3:+72313387 None:intergenic
TATTAAGGAGAAATGGCAGA+GGG 0.641387 4_3:-72314426 Msa0661120:exon
ATGGCATTAATAATTGGGCA+AGG 0.658098 4_3:-72314596 None:intergenic
ACGTATTGGAGAACCAGAAG+AGG 0.658963 4_3:-72312661 Msa0661120:CDS
GTATTAAGGAGAAATGGCAG+AGG 0.659922 4_3:-72314427 Msa0661120:exon
GGAGCAATTAGCAGAGCTAG+GGG 0.662491 4_3:-72313861 Msa0661120:CDS
TTCTCCAATACGTCCAAGAG+GGG 0.662521 4_3:+72312671 None:intergenic
GGACTAATTGTGTTGCACCA+GGG 0.676221 4_3:-72313170 Msa0661120:CDS
GCATTAATAATTGGGCAAGG+AGG 0.678230 4_3:-72314593 None:intergenic
TAAGCATAGGAATGCCACCA+AGG 0.681785 4_3:+72312632 None:intergenic
GGTTCTCCAATACGTCCAAG+AGG 0.697030 4_3:+72312669 None:intergenic
CCTAGCACAAGTGTGTACAG+TGG 0.731390 4_3:+72313838 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAAATAAATAAATGT+CGG + chr4_3:72313098-72313117 None:intergenic 10.0%
!! TTTATTTATGTATGAAATTT+AGG - chr4_3:72313105-72313124 Msa0661120:intron 10.0%
!! ATCAATTAACTACAAAATTT+GGG + chr4_3:72312513-72312532 None:intergenic 15.0%
!!! TATTTTTTTCACCTTTTTAT+AGG - chr4_3:72313048-72313067 Msa0661120:intron 15.0%
!! TAATGACAAAAAAATAATGT+TGG - chr4_3:72314192-72314211 Msa0661120:intron 15.0%
!! GATCAATTAACTACAAAATT+TGG + chr4_3:72312514-72312533 None:intergenic 20.0%
!! TTGTTAAAATATTGTTCCAT+TGG + chr4_3:72312696-72312715 None:intergenic 20.0%
!! TGTATTATTAATTGCTTACA+AGG + chr4_3:72313008-72313027 None:intergenic 20.0%
!! ACTAAACAAATCCTATAAAA+AGG + chr4_3:72313062-72313081 None:intergenic 20.0%
!! ATATATGTAATTTACAATGC+AGG - chr4_3:72313425-72313444 Msa0661120:CDS 20.0%
!!! TAGACACAATCAAATTTTTT+TGG + chr4_3:72313780-72313799 None:intergenic 20.0%
!! TAAAAAACGAGTTTATAAGT+AGG + chr4_3:72313845-72313864 None:intergenic 20.0%
!!! TACTTATAAACTCGTTTTTT+AGG - chr4_3:72313844-72313863 Msa0661120:CDS 20.0%
!!! TTCTAGAAAAACTTTTGATT+TGG - chr4_3:72314497-72314516 Msa0661120:five_prime_UTR 20.0%
! TCAACTAATTTCATGATCTA+TGG + chr4_3:72312451-72312470 None:intergenic 25.0%
! CTTTGACTAATAATAACCAA+TGG - chr4_3:72312677-72312696 Msa0661120:CDS 25.0%
! ATGTGAATTAAACTATGACT+AGG - chr4_3:72312779-72312798 Msa0661120:intron 25.0%
!! GTAAAATCTAGTGATTGTTT+TGG + chr4_3:72313154-72313173 None:intergenic 25.0%
! AAACCAGCTTACAAAAAATT+TGG - chr4_3:72313454-72313473 Msa0661120:CDS 25.0%
! TGACAAAAAAATAATGTTGG+TGG - chr4_3:72314195-72314214 Msa0661120:intron 25.0%
! AAAATGAAAACACGACTTAT+GGG + chr4_3:72314239-72314258 None:intergenic 25.0%
! TAAAATGAAAACACGACTTA+TGG + chr4_3:72314240-72314259 None:intergenic 25.0%
! TTATTTGTGAGAGTTTCATA+TGG - chr4_3:72314439-72314458 Msa0661120:five_prime_UTR 25.0%
! TATTTGTGAGAGTTTCATAT+GGG - chr4_3:72314440-72314459 Msa0661120:five_prime_UTR 25.0%
! CAAATACACATGCTTTAATA+TGG + chr4_3:72314539-72314558 None:intergenic 25.0%
! AAGCATGTGTATTTGTAAAA+AGG - chr4_3:72314544-72314563 Msa0661120:five_prime_UTR 25.0%
AACACATGAAAACTTCTACT+TGG - chr4_3:72312144-72312163 Msa0661120:three_prime_UTR 30.0%
AAATAGTAAGTGGTCTTTGA+AGG - chr4_3:72312286-72312305 Msa0661120:three_prime_UTR 30.0%
AATAGTAAGTGGTCTTTGAA+GGG - chr4_3:72312287-72312306 Msa0661120:three_prime_UTR 30.0%
TTAACTCAAAGCTAATTTCC+TGG - chr4_3:72312575-72312594 Msa0661120:CDS 30.0%
TATGACTAGGTATGATATAG+TGG - chr4_3:72312792-72312811 Msa0661120:intron 30.0%
TCAATGAATGCTTACATCAA+TGG - chr4_3:72312872-72312891 Msa0661120:intron 30.0%
CAATGAATGCTTACATCAAT+GGG - chr4_3:72312873-72312892 Msa0661120:intron 30.0%
ACAAAAGGATACAAAATCAC+TGG - chr4_3:72312898-72312917 Msa0661120:intron 30.0%
TGAAATTTAGGTGAACAATG+TGG - chr4_3:72313117-72313136 Msa0661120:intron 30.0%
GAAATTTAGGTGAACAATGT+GGG - chr4_3:72313118-72313137 Msa0661120:intron 30.0%
AGCAAAAGGTAACTATTTCA+TGG - chr4_3:72313336-72313355 Msa0661120:intron 30.0%
!!! ATGCCAAATTTTTTGTAAGC+TGG + chr4_3:72313460-72313479 None:intergenic 30.0%
AAAAAATTTGGCATGTGAGT+GGG - chr4_3:72313466-72313485 Msa0661120:CDS 30.0%
TAATCCAACTCCTAGAAAAT+CGG - chr4_3:72313798-72313817 Msa0661120:CDS 30.0%
GTCCTATATCGAATGAAATA+TGG + chr4_3:72313870-72313889 None:intergenic 30.0%
!!! ATCTTTTAAGATCTGCAAGA+AGG + chr4_3:72313952-72313971 None:intergenic 30.0%
AGAAGAAATGTCTACAAATC+TGG + chr4_3:72314477-72314496 None:intergenic 30.0%
AAGGGGGTTTATATATGCAT+GGG + chr4_3:72312204-72312223 None:intergenic 35.0%
TAAGGGGGTTTATATATGCA+TGG + chr4_3:72312205-72312224 None:intergenic 35.0%
GGTAGAATCACCAAGTATTA+AGG - chr4_3:72312233-72312252 Msa0661120:three_prime_UTR 35.0%
CACCAAGTATTAAGGAGAAA+TGG - chr4_3:72312241-72312260 Msa0661120:three_prime_UTR 35.0%
! TGCCATTTCTCCTTAATACT+TGG + chr4_3:72312246-72312265 None:intergenic 35.0%
TATTAAGGAGAAATGGCAGA+GGG - chr4_3:72312248-72312267 Msa0661120:three_prime_UTR 35.0%
CAAGTAGCAGAAATAGTAAG+TGG - chr4_3:72312276-72312295 Msa0661120:three_prime_UTR 35.0%
! ATAGTAAGTGGTCTTTGAAG+GGG - chr4_3:72312288-72312307 Msa0661120:three_prime_UTR 35.0%
GCACAAAAGAATAAGAGAAG+AGG + chr4_3:72312373-72312392 None:intergenic 35.0%
GAATTAGACTATTGACAACC+AGG + chr4_3:72312596-72312615 None:intergenic 35.0%
TTACATCAATGGGTCACAAA+AGG - chr4_3:72312883-72312902 Msa0661120:intron 35.0%
TGAGCAAGATTTCTCATTTC+TGG - chr4_3:72313174-72313193 Msa0661120:CDS 35.0%
!! GAGTTTGAAGCTTTGAGTAA+TGG + chr4_3:72313247-72313266 None:intergenic 35.0%
! ATTGTTTTCATGTCCTCCAT+TGG - chr4_3:72313274-72313293 Msa0661120:intron 35.0%
CAAAAAATTTGGCATGTGAG+TGG - chr4_3:72313465-72313484 Msa0661120:CDS 35.0%
AAATTACTTCTGCAGCAAGA+GGG + chr4_3:72313541-72313560 None:intergenic 35.0%
AAAATTACTTCTGCAGCAAG+AGG + chr4_3:72313542-72313561 None:intergenic 35.0%
! TAAAACTAGTTTGTCCGATG+TGG - chr4_3:72313725-72313744 Msa0661120:CDS 35.0%
! AAAACTAGTTTGTCCGATGT+GGG - chr4_3:72313726-72313745 Msa0661120:CDS 35.0%
TTGGTATGTGACTAATGAGT+TGG + chr4_3:72313761-72313780 None:intergenic 35.0%
TAAGTAGGAGACAAGTAACA+CGG + chr4_3:72313830-72313849 None:intergenic 35.0%
GGCCATATTTCATTCGATAT+AGG - chr4_3:72313865-72313884 Msa0661120:CDS 35.0%
! AGACCATTTGTATTGATGGA+GGG - chr4_3:72314090-72314109 Msa0661120:intron 35.0%
TAACCCTCCATCAATACAAA+TGG + chr4_3:72314096-72314115 None:intergenic 35.0%
!! ACAAGTATCGAATACGTATC+TGG + chr4_3:72314583-72314602 None:intergenic 35.0%
ATTCTACCTTCTGGTCTAAG+GGG + chr4_3:72312221-72312240 None:intergenic 40.0%
GATTCTACCTTCTGGTCTAA+GGG + chr4_3:72312222-72312241 None:intergenic 40.0%
TGATTCTACCTTCTGGTCTA+AGG + chr4_3:72312223-72312242 None:intergenic 40.0%
! TACTTGGTGATTCTACCTTC+TGG + chr4_3:72312230-72312249 None:intergenic 40.0%
GTATTAAGGAGAAATGGCAG+AGG - chr4_3:72312247-72312266 Msa0661120:three_prime_UTR 40.0%
AGATAGCAACCCTATTCCTT+TGG + chr4_3:72312339-72312358 None:intergenic 40.0%
! AAGCATCGTGACTATTGACT+AGG + chr4_3:72312620-72312639 None:intergenic 40.0%
CAATAGTCACGATGCTTGTT+TGG - chr4_3:72312623-72312642 Msa0661120:CDS 40.0%
AGAGTCTCTTCTGAGTTCAA+TGG - chr4_3:72312970-72312989 Msa0661120:intron 40.0%
! TTTTCATGTCCTCCATTGGT+GGG - chr4_3:72313278-72313297 Msa0661120:intron 40.0%
ACCATCTATAGTGCAGCAAA+AGG - chr4_3:72313322-72313341 Msa0661120:intron 40.0%
! ACCTTTTGCTGCACTATAGA+TGG + chr4_3:72313326-72313345 None:intergenic 40.0%
AATTACTTCTGCAGCAAGAG+GGG + chr4_3:72313540-72313559 None:intergenic 40.0%
!! TTAGCCGATTTTCTAGGAGT+TGG + chr4_3:72313805-72313824 None:intergenic 40.0%
!! ACGGTATTAGCCGATTTTCT+AGG + chr4_3:72313811-72313830 None:intergenic 40.0%
TTCATTTCAAGAACCCCTCT+TGG - chr4_3:72313990-72314009 Msa0661120:intron 40.0%
GGACAGACCATTTGTATTGA+TGG - chr4_3:72314086-72314105 Msa0661120:intron 40.0%
! CAGACCATTTGTATTGATGG+AGG - chr4_3:72314089-72314108 Msa0661120:intron 40.0%
GTCTCCTTCTACAATAAGCT+AGG - chr4_3:72314126-72314145 Msa0661120:intron 40.0%
CAAGCCTAGCTTATTGTAGA+AGG + chr4_3:72314133-72314152 None:intergenic 40.0%
ATAAACCCCCTTAGACCAGA+AGG - chr4_3:72312212-72312231 Msa0661120:three_prime_UTR 45.0%
TTCTACCTTCTGGTCTAAGG+GGG + chr4_3:72312220-72312239 None:intergenic 45.0%
! ACTGGTGGATCCAAAGGAAT+AGG - chr4_3:72312326-72312345 Msa0661120:three_prime_UTR 45.0%
! CTGGTGGATCCAAAGGAATA+GGG - chr4_3:72312327-72312346 Msa0661120:three_prime_UTR 45.0%
TGGAGCAATTAGCAGAGCTA+GGG - chr4_3:72312812-72312831 Msa0661120:intron 45.0%
!! TGAGTAATGGATGTGCAAGC+TGG + chr4_3:72313234-72313253 None:intergenic 45.0%
! GTTTTCATGTCCTCCATTGG+TGG - chr4_3:72313277-72313296 Msa0661120:intron 45.0%
TTTCATGTCCTCCATTGGTG+GGG - chr4_3:72313279-72313298 Msa0661120:intron 45.0%
GGGGTAGCATCACTAAATGT+AGG - chr4_3:72313298-72313317 Msa0661120:intron 45.0%
!! AGTGGGCTAGAGACAACATA+AGG - chr4_3:72313483-72313502 Msa0661120:CDS 45.0%
AGGACTAATTGTGTTGCACC+AGG - chr4_3:72313503-72313522 Msa0661120:CDS 45.0%
GGACTAATTGTGTTGCACCA+GGG - chr4_3:72313504-72313523 Msa0661120:CDS 45.0%
TTCTCCAATACGTCCAAGAG+GGG + chr4_3:72314006-72314025 None:intergenic 45.0%
GTTCTCCAATACGTCCAAGA+GGG + chr4_3:72314007-72314026 None:intergenic 45.0%
ACGTATTGGAGAACCAGAAG+AGG - chr4_3:72314013-72314032 Msa0661120:intron 45.0%
TAAGCATAGGAATGCCACCA+AGG + chr4_3:72314045-72314064 None:intergenic 45.0%
GGTCTGTCCTGTTATGAAAG+AGG + chr4_3:72314075-72314094 None:intergenic 45.0%
TTTGAAGGGGATGACAGCTC+TGG - chr4_3:72312301-72312320 Msa0661120:three_prime_UTR 50.0%
GTGGAGCAATTAGCAGAGCT+AGG - chr4_3:72312811-72312830 Msa0661120:intron 50.0%
GGAGCAATTAGCAGAGCTAG+GGG - chr4_3:72312813-72312832 Msa0661120:intron 50.0%
CCTAGCACAAGTGTGTACAG+TGG + chr4_3:72312839-72312858 None:intergenic 50.0%
CCACTGTACACACTTGTGCT+AGG - chr4_3:72312836-72312855 Msa0661120:intron 50.0%
GTCATCTCGTGCACAAAGAG+AGG - chr4_3:72312936-72312955 Msa0661120:intron 50.0%
TAGTGATGCTACCCCACCAA+TGG + chr4_3:72313293-72313312 None:intergenic 50.0%
! ACTAGTTTGTCCGATGTGGG+AGG - chr4_3:72313729-72313748 Msa0661120:CDS 50.0%
!! TTGGACTAGTCCTCCCACAT+CGG + chr4_3:72313742-72313761 None:intergenic 50.0%
AGAACCCCTCTTGGACGTAT+TGG - chr4_3:72313999-72314018 Msa0661120:intron 50.0%
GGTTCTCCAATACGTCCAAG+AGG + chr4_3:72314008-72314027 None:intergenic 50.0%
!! ACCAGAAGAGGTGTCTTCCT+TGG - chr4_3:72314025-72314044 Msa0661120:intron 50.0%
ACCAAGGAAGACACCTCTTC+TGG + chr4_3:72314029-72314048 None:intergenic 50.0%
!! AGAAGAGGTGTCTTCCTTGG+TGG - chr4_3:72314028-72314047 Msa0661120:intron 50.0%
AAGAGGCTGCAGGTAAGCAT+AGG + chr4_3:72314058-72314077 None:intergenic 50.0%
CCTGTTATGAAAGAGGCTGC+AGG + chr4_3:72314068-72314087 None:intergenic 50.0%
CCTGCAGCCTCTTTCATAAC+AGG - chr4_3:72314065-72314084 Msa0661120:intron 50.0%
! ATGACAGCTCTGGTCACTGG+TGG - chr4_3:72312311-72312330 Msa0661120:three_prime_UTR 55.0%
! CTGGTCACTGGTGGATCCAA+AGG - chr4_3:72312320-72312339 Msa0661120:three_prime_UTR 55.0%
TGATGCTACCCCACCAATGG+AGG + chr4_3:72313290-72313309 None:intergenic 55.0%
! GCAGCAAGAGGGGTTCTGAT+TGG + chr4_3:72313530-72313549 None:intergenic 55.0%
! GGGATGACAGCTCTGGTCAC+TGG - chr4_3:72312308-72312327 Msa0661120:three_prime_UTR 60.0%
! AGAGGGGTTCTGATTGGCCC+TGG + chr4_3:72313524-72313543 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 72312093 72314603 72312093 ID=Msa0661120;Name=Msa0661120
chr4_3 mRNA 72312093 72314603 72312093 ID=Msa0661120-mRNA-1;Parent=Msa0661120;Name=Msa0661120-mRNA-1;_AED=0.03;_eAED=0.03;_QI=167|1|1|1|1|1|5|461|267
chr4_3 exon 72312093 72312736 72312093 ID=Msa0661120-mRNA-1:exon:21685;Parent=Msa0661120-mRNA-1
chr4_3 exon 72313143 72313249 72313143 ID=Msa0661120-mRNA-1:exon:21684;Parent=Msa0661120-mRNA-1
chr4_3 exon 72313353 72313569 72313353 ID=Msa0661120-mRNA-1:exon:21683;Parent=Msa0661120-mRNA-1
chr4_3 exon 72313688 72313895 72313688 ID=Msa0661120-mRNA-1:exon:21682;Parent=Msa0661120-mRNA-1
chr4_3 exon 72314348 72314603 72314348 ID=Msa0661120-mRNA-1:exon:21681;Parent=Msa0661120-mRNA-1
chr4_3 five_prime_UTR 72314437 72314603 72314437 ID=Msa0661120-mRNA-1:five_prime_utr;Parent=Msa0661120-mRNA-1
chr4_3 CDS 72314348 72314436 72314348 ID=Msa0661120-mRNA-1:cds;Parent=Msa0661120-mRNA-1
chr4_3 CDS 72313688 72313895 72313688 ID=Msa0661120-mRNA-1:cds;Parent=Msa0661120-mRNA-1
chr4_3 CDS 72313353 72313569 72313353 ID=Msa0661120-mRNA-1:cds;Parent=Msa0661120-mRNA-1
chr4_3 CDS 72313143 72313249 72313143 ID=Msa0661120-mRNA-1:cds;Parent=Msa0661120-mRNA-1
chr4_3 CDS 72312554 72312736 72312554 ID=Msa0661120-mRNA-1:cds;Parent=Msa0661120-mRNA-1
chr4_3 three_prime_UTR 72312093 72312553 72312093 ID=Msa0661120-mRNA-1:three_prime_utr;Parent=Msa0661120-mRNA-1
Gene Sequence

>Msa0661120

ATGGCAGAGGGAAACACAAGTAGCAGAAATAGTAAGTGGTCTTTGAAGGGGATGACAGCTCTGGTCACTGGTGGATCCAAAGGAATAGGGTATGATATAGTGGAGCAATTAGCAGAGCTAGGGGCCACTGTACACACTTGTGCTAGGAATGAAGCTCAACTCAATGAATGCTTACATCAATGGGTCACAAAAGGATACAAAATCACTGGTTCAGTCTGTGATGTGTCATCTCGTGCACAAAGAGAGGAGCTCATAGCTAGAGTCTCTTCTGAGTTCAATGGCAAACTCAATATCCTTGTGAACAATGTGGGAACAAACATACCAAAACAATCACTAGATTTTACTGAGCAAGATTTCTCATTTCTGGTGAATACAAATCTTGAATCTGCTTTTCACATAAGCCAGCTTGCACATCCATTACTCAAAGCTTCAAACTCTGCAAGCATTGTTTTCATGTCCTCCATTGGTGGGGTAGCATCACTAAATGTAGGAACCATCTATAGTGCAGCAAAAGGAGCAATAAACCAGCTTACAAAAAATTTGGCATGTGAGTGGGCTAGAGACAACATAAGGACTAATTGTGTTGCACCAGGGCCAATCAGAACCCCTCTTGCTGCAGAAATCTTAAAAGATGAAAAACTTCTCGATGCTTTCATTTCAAGAACCCCTCTTGGACGTATTGGAGAACCAGAAGAGGTGTCTTCCTTGGTGGCATTCCTATGCTTACCTGCAGCCTCTTTCATAACAGGACAGACCATTTGTATTGATGGAGGGTTAACTGTCAACTGTCTCCTTCTACAATAA
Protein sequence

>Msa0661120

MAEGNTSSRNSKWSLKGMTALVTGGSKGIGYDIVEQLAELGATVHTCARNEAQLNECLHQWVTKGYKITGSVCDVSSRAQREELIARVSSEFNGKLNILVNNVGTNIPKQSLDFTEQDFSFLVNTNLESAFHISQLAHPLLKASNSASIVFMSSIGGVASLNVGTIYSAAKGAINQLTKNLACEWARDNIRTNCVAPGPIRTPLAAEILKDEKLLDAFISRTPLGRIGEPEEVSSLVAFLCLPAASFITGQTICIDGGLTVNCLLLQ*