AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0684320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684320 MtrunA17_Chr4g0032181 93.671 237 15 0 31 267 17 253 5.64e-165 457
Msa0684320 MtrunA17_Chr3g0112261 61.004 259 101 0 3 261 7 265 1.00e-121 348
Msa0684320 MtrunA17_Chr3g0112271 62.698 252 94 0 11 262 13 264 1.71e-119 342
Msa0684320 MtrunA17_Chr3g0112151 59.160 262 107 0 1 262 72 333 9.49e-118 340
Msa0684320 MtrunA17_Chr4g0032141 62.451 253 95 0 10 262 51 303 1.08e-115 334
Msa0684320 MtrunA17_Chr3g0112171 59.274 248 101 0 15 262 15 262 5.80e-112 323
Msa0684320 MtrunA17_Chr3g0112141 59.677 248 100 0 15 262 17 264 7.70e-111 320
Msa0684320 MtrunA17_Chr7g0239511 55.805 267 111 3 1 261 1 266 1.23e-105 307
Msa0684320 MtrunA17_Chr7g0239451 55.094 265 114 2 1 260 1 265 1.50e-104 304
Msa0684320 MtrunA17_Chr7g0239501 54.135 266 117 3 1 261 1 266 1.28e-101 297
Msa0684320 MtrunA17_Chr7g0239481 53.232 263 119 1 1 259 1 263 1.16e-100 294
Msa0684320 MtrunA17_Chr7g0239491 53.232 263 120 1 1 260 1 263 1.09e-99 292
Msa0684320 MtrunA17_Chr7g0239461 49.242 264 81 4 1 260 1 215 9.07e-83 247
Msa0684320 MtrunA17_Chr4g0075531 33.333 246 159 4 15 259 7 248 9.49e-34 122
Msa0684320 MtrunA17_Chr3g0122901 33.740 246 152 7 21 261 81 320 4.00e-30 115
Msa0684320 MtrunA17_Chr3g0112321 55.102 98 28 2 148 242 3 87 1.17e-29 107
Msa0684320 MtrunA17_Chr7g0259521 32.400 250 161 5 15 258 6 253 1.50e-27 107
Msa0684320 MtrunA17_Chr7g0259531 29.880 251 168 4 15 259 11 259 1.24e-25 102
Msa0684320 MtrunA17_Chr8g0357481 31.008 258 159 6 15 259 14 265 2.14e-25 102
Msa0684320 MtrunA17_Chr4g0074511 29.811 265 174 6 8 263 33 294 2.38e-25 101
Msa0684320 MtrunA17_Chr0c30g0494101 29.699 266 171 7 8 263 33 292 1.11e-23 97.1
Msa0684320 MtrunA17_Chr4g0028921 28.346 254 175 5 15 261 11 264 7.17e-22 92.0
Msa0684320 MtrunA17_Chr4g0044111 30.078 256 163 8 15 261 13 261 2.15e-21 90.5
Msa0684320 MtrunA17_Chr4g0028911 27.953 254 176 5 15 261 11 264 2.55e-21 90.5
Msa0684320 MtrunA17_Chr4g0026001 30.712 267 160 12 15 267 14 269 6.23e-21 89.4
Msa0684320 MtrunA17_Chr7g0266981 29.297 256 165 8 15 261 13 261 7.39e-21 89.4
Msa0684320 MtrunA17_Chr1g0182511 31.890 254 158 7 15 258 14 262 2.51e-20 87.4
Msa0684320 MtrunA17_Chr6g0459281 28.409 264 177 6 1 257 1 259 4.96e-20 87.0
Msa0684320 MtrunA17_Chr3g0084871 30.370 270 169 10 5 260 6 270 8.37e-20 86.7
Msa0684320 MtrunA17_Chr7g0244061 27.491 291 173 8 6 266 20 302 2.20e-19 85.5
Msa0684320 MtrunA17_Chr6g0459301 28.195 266 180 7 1 259 1 262 2.79e-19 84.7
Msa0684320 MtrunA17_Chr7g0272961 29.183 257 171 6 15 263 40 293 4.03e-19 84.7
Msa0684320 MtrunA17_Chr4g0025981 27.863 262 156 7 16 261 42 286 6.62e-19 84.7
Msa0684320 MtrunA17_Chr7g0266891 28.516 256 167 8 15 261 7 255 9.30e-19 83.6
Msa0684320 MtrunA17_Chr2g0324021 28.517 263 174 8 11 264 2 259 3.54e-18 81.6
Msa0684320 MtrunA17_Chr6g0459261 28.571 266 178 7 1 259 13 273 4.24e-18 81.6
Msa0684320 MtrunA17_Chr1g0171831 26.295 251 169 8 15 251 14 262 5.77e-18 81.3
Msa0684320 MtrunA17_Chr6g0459351 27.273 264 180 7 1 257 1 259 2.16e-17 79.7
Msa0684320 MtrunA17_Chr1g0191751 28.405 257 171 8 15 262 101 353 2.41e-17 80.5
Msa0684320 MtrunA17_Chr6g0459271 28.947 266 177 7 1 259 1 261 3.64e-17 79.0
Msa0684320 MtrunA17_Chr4g0044131 25.573 262 178 8 15 265 8 263 4.56e-16 75.9
Msa0684320 MtrunA17_Chr7g0252031 26.923 260 169 7 15 260 35 287 7.89e-16 75.9
Msa0684320 MtrunA17_Chr4g0025971 26.357 258 177 6 10 261 9 259 8.81e-16 75.1
Msa0684320 MtrunA17_Chr4g0040371 27.857 280 162 13 5 260 8 271 9.22e-16 75.5
Msa0684320 MtrunA17_Chr7g0270441 27.027 259 176 8 11 261 2 255 1.47e-15 74.3
Msa0684320 MtrunA17_Chr7g0268591 25.283 265 180 6 8 260 26 284 2.39e-15 74.3
Msa0684320 MtrunA17_Chr6g0459331 28.195 266 179 7 1 259 1 261 2.62e-15 73.9
Msa0684320 MtrunA17_Chr7g0249571 28.646 192 119 4 22 202 45 229 3.79e-15 73.9
Msa0684320 MtrunA17_Chr8g0381191 29.167 216 142 6 17 225 47 258 9.03e-14 70.1
Msa0684320 MtrunA17_Chr6g0466681 28.571 252 154 7 15 250 64 305 3.52e-13 68.6
Msa0684320 MtrunA17_Chr7g0270451 28.125 256 174 8 12 261 3 254 2.26e-12 65.5
Msa0684320 MtrunA17_Chr7g0266991 30.288 208 110 7 13 209 18 201 2.72e-12 65.1
Msa0684320 MtrunA17_Chr7g0267011 26.596 188 133 4 15 200 8 192 2.84e-12 63.9
Msa0684320 MtrunA17_Chr4g0038121 30.526 190 121 7 76 259 186 370 4.26e-12 65.5
Msa0684320 MtrunA17_Chr7g0267001 24.231 260 166 8 15 265 8 245 1.42e-11 63.2
Msa0684320 MtrunA17_Chr7g0234681 31.148 183 117 7 82 259 192 370 2.39e-11 63.2
Msa0684320 MtrunA17_Chr4g0032171 93.333 30 2 0 1 30 4 33 2.92e-11 57.4
Msa0684320 MtrunA17_Chr7g0247011 26.939 245 120 6 20 208 9 250 5.68e-11 61.6
Msa0684320 MtrunA17_Chr4g0076931 28.272 191 133 3 19 206 10 199 8.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684320 AT1G07440.1 63.602 261 94 1 7 267 4 263 5.76e-123 351
Msa0684320 AT2G29340.2 62.016 258 98 0 10 267 2 259 1.05e-119 343
Msa0684320 AT2G29150.1 63.878 263 93 2 1 262 1 262 1.78e-119 342
Msa0684320 AT5G06060.1 61.265 253 98 0 10 262 4 256 2.20e-119 342
Msa0684320 AT2G29340.1 62.016 258 98 0 10 267 2 259 4.34e-119 343
Msa0684320 AT2G29350.1 63.740 262 94 1 1 262 1 261 2.43e-118 340
Msa0684320 AT2G29360.1 63.878 263 94 1 1 262 1 263 3.41e-117 337
Msa0684320 AT2G29290.2 62.791 258 96 0 10 267 2 259 5.03e-117 336
Msa0684320 AT2G30670.1 61.660 253 97 0 10 262 2 254 3.62e-115 331
Msa0684320 AT2G29310.1 60.392 255 101 0 10 264 2 256 4.17e-113 326
Msa0684320 AT1G07450.1 63.347 251 91 1 12 262 5 254 3.41e-111 321
Msa0684320 AT2G29370.1 61.217 263 101 1 1 262 1 263 4.59e-111 321
Msa0684320 AT2G29330.1 58.140 258 108 0 10 267 2 259 5.25e-110 318
Msa0684320 AT2G29260.1 59.363 251 102 0 12 262 65 315 5.52e-110 320
Msa0684320 AT2G29320.1 58.594 256 105 1 13 267 11 266 2.49e-108 314
Msa0684320 AT2G29300.1 58.103 253 105 1 10 261 2 254 1.18e-106 310
Msa0684320 AT2G29300.2 56.977 258 110 1 10 266 2 259 2.15e-105 307
Msa0684320 AT2G29290.1 62.553 235 88 0 33 267 8 242 1.47e-104 304
Msa0684320 AT2G29350.3 63.677 223 80 1 40 262 2 223 1.43e-100 293
Msa0684320 AT2G29310.2 60.345 232 92 0 31 262 21 252 4.76e-100 293
Msa0684320 AT2G29320.2 57.143 238 101 1 31 267 11 248 1.68e-96 283
Msa0684320 AT2G29350.2 63.942 208 75 0 1 208 1 208 8.26e-92 271
Msa0684320 AT2G29340.3 58.500 200 83 0 10 209 2 201 4.34e-86 255
Msa0684320 AT2G29340.4 58.500 200 83 0 10 209 2 201 4.34e-86 255
Msa0684320 AT2G29310.3 56.500 200 87 0 10 209 2 201 2.01e-81 243
Msa0684320 AT2G29320.3 56.545 191 82 1 13 202 11 201 1.26e-75 229
Msa0684320 AT1G07440.2 60.843 166 65 0 7 172 4 169 1.36e-71 218
Msa0684320 AT2G29310.4 55.307 179 80 0 31 209 21 199 2.34e-68 210
Msa0684320 AT2G29170.1 61.765 102 38 1 1 101 1 102 4.91e-38 129
Msa0684320 AT4G05530.1 31.707 246 163 4 15 259 9 250 1.87e-30 114
Msa0684320 AT3G03980.1 31.852 270 163 7 5 258 4 268 1.05e-28 110
Msa0684320 AT1G24360.1 32.787 244 157 6 21 261 80 319 7.77e-28 109
Msa0684320 AT3G04000.1 32.075 265 160 8 15 265 14 272 2.22e-27 107
Msa0684320 AT4G13180.1 31.559 263 172 6 5 261 3 263 2.43e-27 106
Msa0684320 AT3G55310.1 30.078 256 168 7 15 261 17 270 3.39e-27 106
Msa0684320 AT3G55290.1 29.297 256 170 7 15 261 18 271 8.90e-26 102
Msa0684320 AT3G55290.2 29.297 256 170 7 15 261 17 270 9.39e-26 102
Msa0684320 AT1G63380.3 30.739 257 166 7 15 261 15 269 1.04e-25 102
Msa0684320 AT5G18210.1 30.268 261 159 6 14 259 7 259 1.07e-25 102
Msa0684320 AT1G63380.1 30.739 257 166 7 15 261 17 271 1.08e-25 102
Msa0684320 AT1G63380.4 30.739 257 166 7 15 261 19 273 1.14e-25 102
Msa0684320 AT1G63380.2 30.739 257 166 7 15 261 22 276 1.55e-25 102
Msa0684320 AT3G12800.1 28.346 254 172 4 15 259 10 262 1.24e-24 100
Msa0684320 AT3G05260.1 32.707 266 165 8 7 262 27 288 1.33e-24 100
Msa0684320 AT1G62610.2 29.961 257 168 7 15 261 13 267 3.42e-24 98.6
Msa0684320 AT1G62610.1 29.961 257 168 7 15 261 14 268 3.62e-24 98.6
Msa0684320 AT1G62610.4 29.961 257 168 7 15 261 19 273 3.84e-24 98.6
Msa0684320 AT1G62610.3 29.961 257 168 7 15 261 15 269 3.93e-24 98.6
Msa0684320 AT1G54870.2 32.061 262 155 10 15 262 84 336 9.44e-24 98.6
Msa0684320 AT1G54870.1 32.061 262 155 10 15 262 82 334 1.06e-23 98.6
Msa0684320 AT5G18210.2 30.453 243 146 6 14 241 7 241 1.26e-22 94.4
Msa0684320 AT3G46170.1 27.344 256 175 7 15 261 26 279 4.37e-21 90.5
Msa0684320 AT2G17845.1 27.734 256 171 7 15 258 47 300 4.45e-21 90.9
Msa0684320 AT2G47120.1 27.519 258 173 9 11 261 2 252 9.55e-19 83.6
Msa0684320 AT2G47120.2 27.559 254 170 9 15 261 7 253 1.66e-18 82.8
Msa0684320 AT3G51680.1 27.336 289 182 10 1 266 20 303 3.22e-18 82.8
Msa0684320 AT3G42960.1 29.213 267 165 9 10 260 3 261 5.80e-18 81.6
Msa0684320 AT3G26760.1 28.044 271 170 9 5 260 30 290 2.27e-17 80.5
Msa0684320 AT4G03140.1 25.781 256 176 6 15 260 78 329 5.02e-17 80.1
Msa0684320 AT3G06060.1 28.571 217 144 5 9 220 31 241 6.08e-15 73.9
Msa0684320 AT3G06060.2 28.571 217 144 5 9 220 48 258 7.25e-15 73.9
Msa0684320 AT1G52340.1 27.106 273 157 8 15 260 18 275 1.22e-14 72.4
Msa0684320 AT2G47130.1 26.562 256 179 7 11 261 2 253 1.37e-14 72.0
Msa0684320 AT3G26770.1 27.273 264 163 8 15 260 41 293 9.02e-14 70.5
Msa0684320 AT5G19200.1 27.619 210 143 4 15 220 35 239 1.72e-13 69.7
Msa0684320 AT1G10310.1 31.551 187 124 4 19 204 19 202 2.66e-12 65.5
Msa0684320 AT3G03330.1 26.531 196 135 5 14 202 40 233 3.69e-12 65.9
Msa0684320 AT5G65205.1 26.596 188 127 5 21 205 12 191 5.86e-11 62.0

Find 83 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCAAGATTTCTCATTTC+TGG 0.136218 4_4:+47660893 Msa0684320:CDS
TGATTCTACCTTCTGGTCTA+AGG 0.215896 4_4:-47659939 None:intergenic
TAAAATGAAAACACGACTTA+TGG 0.217681 4_4:-47661936 None:intergenic
AAGCATGTGTATTTGTAAAA+AGG 0.219514 4_4:+47662243 Msa0684320:three_prime_UTR
CAAATACACATGCTTTAATA+TGG 0.241647 4_4:-47662235 None:intergenic
GGTAGAATCACCAAGTATTA+AGG 0.311024 4_4:+47659952 Msa0684320:five_prime_UTR
CAAGCCTAGCTTATTGTAGA+AGG 0.334137 4_4:-47661829 None:intergenic
GTAAAATCTAGTGATTGTTT+TGG 0.345117 4_4:-47660870 None:intergenic
CCTGCAGCCTCTTTCATAAC+AGG 0.358569 4_4:+47661764 Msa0684320:CDS
AAAATGAAAACACGACTTAT+GGG 0.374397 4_4:-47661935 None:intergenic
TACTTGGTGATTCTACCTTC+TGG 0.374722 4_4:-47659946 None:intergenic
GGACAGACCATTTGTATTGA+TGG 0.378202 4_4:+47661785 Msa0684320:CDS
GCTGCAAGAGGGGTTCTGAT+TGG 0.385802 4_4:-47661248 None:intergenic
TGCCATTTCTCCTTAATACT+TGG 0.386036 4_4:-47659962 None:intergenic
ACTGGTGGATCCAAAGGAAT+AGG 0.388939 4_4:+47660045 Msa0684320:CDS
AGAACCCCTCTTGGACGTAT+TGG 0.397142 4_4:+47661698 Msa0684320:CDS
AGATAGCAACCCTATTCCTT+TGG 0.397711 4_4:-47660055 None:intergenic
TCAATGAATGCTTACATCAA+TGG 0.398769 4_4:+47660591 Msa0684320:CDS
AGAAGAAATGTCTACAAATC+TGG 0.410397 4_4:-47662173 None:intergenic
CACCAAGTATTAAGGAGAAA+TGG 0.414952 4_4:+47659960 Msa0684320:exon
ACAAGTATCGAATACGTATC+TGG 0.415745 4_4:-47662279 None:intergenic
CTCATTTCAAGAACCCCTCT+TGG 0.419602 4_4:+47661689 Msa0684320:CDS
GATTCTACCTTCTGGTCTAA+GGG 0.431428 4_4:-47659938 None:intergenic
CAATGAATGCTTACATCAAT+GGG 0.449372 4_4:+47660592 Msa0684320:CDS
ACCAAGGAAGACACCTCTTC+TGG 0.466532 4_4:-47661725 None:intergenic
AACACATGAAAACTTCTACT+TGG 0.466597 4_4:+47659863 Msa0684320:five_prime_UTR
GAGTTTGAAGCTTTGAGTAA+TGG 0.471691 4_4:-47660963 None:intergenic
TATGACTAGGTATGATATAG+TGG 0.472016 4_4:+47660511 Msa0684320:intron
AAATAGTAAGTGGTCTTTGA+AGG 0.483818 4_4:+47660005 Msa0684320:CDS
TTATTTGTGAGAGTTTCATA+TGG 0.485228 4_4:+47662138 Msa0684320:three_prime_UTR
ACCAGAAGAGGTGTCTTCCT+TGG 0.491068 4_4:+47661724 Msa0684320:CDS
TGGAGCAATTAGCAGAGCTA+GGG 0.491202 4_4:+47660531 Msa0684320:CDS
AGAGGGGTTCTGATTGGCCC+TGG 0.498674 4_4:-47661242 None:intergenic
ATGACAGCTCTGGTCACTGG+TGG 0.504668 4_4:+47660030 Msa0684320:CDS
ACCATCTATAGTGCAGCAAA+AGG 0.506661 4_4:+47661041 Msa0684320:CDS
TAACCCTCCATCAATACAAA+TGG 0.507511 4_4:-47661792 None:intergenic
TATTTGTGAGAGTTTCATAT+GGG 0.511753 4_4:+47662139 Msa0684320:three_prime_UTR
TTTGAAGGGGATGACAGCTC+TGG 0.513224 4_4:+47660020 Msa0684320:CDS
GGGATGACAGCTCTGGTCAC+TGG 0.516153 4_4:+47660027 Msa0684320:CDS
TGAGTAATGGATGTGCAAGC+TGG 0.522910 4_4:-47660950 None:intergenic
AATAGTAAGTGGTCTTTGAA+GGG 0.525324 4_4:+47660006 Msa0684320:CDS
GTGGAGCAATTAGCAGAGCT+AGG 0.526426 4_4:+47660530 Msa0684320:CDS
AGGACTAATTGTGTTGCACC+AGG 0.529569 4_4:+47661224 Msa0684320:CDS
TTACATCAATGGGTCACAAA+AGG 0.532043 4_4:+47660602 Msa0684320:CDS
CAAGTAGCAGAAATAGTAAG+TGG 0.538795 4_4:+47659995 Msa0684320:CDS
CTGGTCACTGGTGGATCCAA+AGG 0.539538 4_4:+47660039 Msa0684320:CDS
AGAGTCTCTTCTGAGTTCAA+TGG 0.543714 4_4:+47660689 Msa0684320:CDS
GGGGTAGCATCACTAAATGT+AGG 0.544472 4_4:+47661017 Msa0684320:CDS
ACAAAAGGATACAAAATCAC+TGG 0.547408 4_4:+47660617 Msa0684320:CDS
CAGACCATTTGTATTGATGG+AGG 0.547706 4_4:+47661788 Msa0684320:CDS
CCTGTTATGAAAGAGGCTGC+AGG 0.552564 4_4:-47661764 None:intergenic
CTGGTGGATCCAAAGGAATA+GGG 0.554228 4_4:+47660046 Msa0684320:CDS
CCACTGTACACACTTGTGCT+AGG 0.562221 4_4:+47660555 Msa0684320:CDS
AGACCATTTGTATTGATGGA+GGG 0.569442 4_4:+47661789 Msa0684320:CDS
TTCTACCTTCTGGTCTAAGG+GGG 0.569545 4_4:-47659936 None:intergenic
TTTCATGTCCTCCATTGTTG+GGG 0.575336 4_4:+47660998 Msa0684320:CDS
ATTCTACCTTCTGGTCTAAG+GGG 0.575493 4_4:-47659937 None:intergenic
GAAATTTAGGTGAACAATGT+GGG 0.576001 4_4:+47660837 Msa0684320:intron
AAATTACTTCTGCTGCAAGA+GGG 0.579918 4_4:-47661259 None:intergenic
GTCTCCTTCTACAATAAGCT+AGG 0.587750 4_4:+47661825 Msa0684320:exon
AGTGGGCTAGAGACAACATA+AGG 0.591685 4_4:+47661204 Msa0684320:CDS
AAAATTACTTCTGCTGCAAG+AGG 0.594537 4_4:-47661260 None:intergenic
GTTCTCCAATACGTCCAAGA+GGG 0.595929 4_4:-47661703 None:intergenic
AGAAGAGGTGTCTTCCTTGG+TGG 0.599777 4_4:+47661727 Msa0684320:CDS
TGAAATTTAGGTGAACAATG+TGG 0.603162 4_4:+47660836 Msa0684320:intron
AATTACTTCTGCTGCAAGAG+GGG 0.604432 4_4:-47661258 None:intergenic
GTCATCTCGTGCACAAAGAG+AGG 0.604943 4_4:+47660655 Msa0684320:CDS
ATAGTAAGTGGTCTTTGAAG+GGG 0.610704 4_4:+47660007 Msa0684320:CDS
GGTCTGTCCTGTTATGAAAG+AGG 0.618109 4_4:-47661771 None:intergenic
AAGAGGCTGCAGGTAAGCAG+AGG 0.623518 4_4:-47661754 None:intergenic
TAAGCAGAGGAATGCCACCA+AGG 0.632879 4_4:-47661741 None:intergenic
TAGTGATGCTACCCCAACAA+TGG 0.638650 4_4:-47661009 None:intergenic
TATTAAGGAGAAATGGCAGA+GGG 0.641387 4_4:+47659967 Msa0684320:exon
ATGGCATTAATAATTGGGCA+AGG 0.658098 4_4:+47659797 None:intergenic
ACGTATTGGAGAACCAGAAG+AGG 0.658963 4_4:+47661712 Msa0684320:CDS
GTATTAAGGAGAAATGGCAG+AGG 0.659922 4_4:+47659966 Msa0684320:exon
GGAGCAATTAGCAGAGCTAG+GGG 0.662491 4_4:+47660532 Msa0684320:CDS
TTCTCCAATACGTCCAAGAG+GGG 0.662521 4_4:-47661702 None:intergenic
TGATGCTACCCCAACAATGG+AGG 0.664800 4_4:-47661006 None:intergenic
GGACTAATTGTGTTGCACCA+GGG 0.676221 4_4:+47661225 Msa0684320:CDS
GCATTAATAATTGGGCAAGG+AGG 0.678230 4_4:+47659800 None:intergenic
GGTTCTCCAATACGTCCAAG+AGG 0.697030 4_4:-47661704 None:intergenic
CCTAGCACAAGTGTGTACAG+TGG 0.731390 4_4:-47660555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAAATAAATAAATGT+CGG - chr4_4:47660817-47660836 None:intergenic 10.0%
!! TTTATTTATGTATGAAATTT+AGG + chr4_4:47660824-47660843 Msa0684320:intron 10.0%
!! ATCAATTAACTACAAAATTT+GGG - chr4_4:47660232-47660251 None:intergenic 15.0%
!!! TATTTTTTTCACCTTTTTAT+AGG + chr4_4:47660767-47660786 Msa0684320:intron 15.0%
!! ACTATATAAATCCTATAAAA+AGG - chr4_4:47660781-47660800 None:intergenic 15.0%
!! CATATTTATTGAATGAAATA+TGG - chr4_4:47661594-47661613 None:intergenic 15.0%
!! TAATGACAAAAAAATAATGT+TGG + chr4_4:47661891-47661910 Msa0684320:three_prime_UTR 15.0%
!! GATCAATTAACTACAAAATT+TGG - chr4_4:47660233-47660252 None:intergenic 20.0%
!!! AATTAGCTTTGAGTTAATTT+AGG - chr4_4:47660289-47660308 None:intergenic 20.0%
!!! TATTGACTAATAATAACCAA+TGG + chr4_4:47660396-47660415 Msa0684320:intron 20.0%
!! TTGTTAAAATATTGTTCCAT+TGG - chr4_4:47660415-47660434 None:intergenic 20.0%
!! TGTATTATTAATTGCTTACA+AGG - chr4_4:47660727-47660746 None:intergenic 20.0%
!! TTTATCAACAAATATGTACA+TGG - chr4_4:47661367-47661386 None:intergenic 20.0%
!! CTAAAAACGAGTTTATAAAT+GGG - chr4_4:47661570-47661589 None:intergenic 20.0%
!!! TTCTAGAAAAACTTTTGATT+TGG + chr4_4:47662196-47662215 Msa0684320:three_prime_UTR 20.0%
! TCAACTAATTTCATGATCTA+TGG - chr4_4:47660170-47660189 None:intergenic 25.0%
! ATGTGAATTAAACTATGACT+AGG + chr4_4:47660498-47660517 Msa0684320:intron 25.0%
!! GTAAAATCTAGTGATTGTTT+TGG - chr4_4:47660873-47660892 None:intergenic 25.0%
! ATATATGTAATTGACAATGC+AGG + chr4_4:47661146-47661165 Msa0684320:intron 25.0%
! AAACCAGCTTACAAAAAATT+TGG + chr4_4:47661175-47661194 Msa0684320:CDS 25.0%
!!! TGGACACAATCTAAATTTTT+TGG - chr4_4:47661501-47661520 None:intergenic 25.0%
! CCTAAAAACGAGTTTATAAA+TGG - chr4_4:47661571-47661590 None:intergenic 25.0%
!!! CCATTTATAAACTCGTTTTT+AGG + chr4_4:47661568-47661587 Msa0684320:intron 25.0%
! TGACAAAAAAATAATGTTGG+TGG + chr4_4:47661894-47661913 Msa0684320:three_prime_UTR 25.0%
! AAAATGAAAACACGACTTAT+GGG - chr4_4:47661938-47661957 None:intergenic 25.0%
! TAAAATGAAAACACGACTTA+TGG - chr4_4:47661939-47661958 None:intergenic 25.0%
! TTATTTGTGAGAGTTTCATA+TGG + chr4_4:47662138-47662157 Msa0684320:three_prime_UTR 25.0%
! TATTTGTGAGAGTTTCATAT+GGG + chr4_4:47662139-47662158 Msa0684320:three_prime_UTR 25.0%
! CAAATACACATGCTTTAATA+TGG - chr4_4:47662238-47662257 None:intergenic 25.0%
! AAGCATGTGTATTTGTAAAA+AGG + chr4_4:47662243-47662262 Msa0684320:three_prime_UTR 25.0%
AACACATGAAAACTTCTACT+TGG + chr4_4:47659863-47659882 Msa0684320:five_prime_UTR 30.0%
AAATAGTAAGTGGTCTTTGA+AGG + chr4_4:47660005-47660024 Msa0684320:CDS 30.0%
AATAGTAAGTGGTCTTTGAA+GGG + chr4_4:47660006-47660025 Msa0684320:CDS 30.0%
GAATTAGACTATTGACAACT+AGG - chr4_4:47660313-47660332 None:intergenic 30.0%
TATGACTAGGTATGATATAG+TGG + chr4_4:47660511-47660530 Msa0684320:intron 30.0%
TCAATGAATGCTTACATCAA+TGG + chr4_4:47660591-47660610 Msa0684320:CDS 30.0%
CAATGAATGCTTACATCAAT+GGG + chr4_4:47660592-47660611 Msa0684320:CDS 30.0%
ACAAAAGGATACAAAATCAC+TGG + chr4_4:47660617-47660636 Msa0684320:CDS 30.0%
TGAAATTTAGGTGAACAATG+TGG + chr4_4:47660836-47660855 Msa0684320:intron 30.0%
GAAATTTAGGTGAACAATGT+GGG + chr4_4:47660837-47660856 Msa0684320:intron 30.0%
!!! TAGTGATTGTTTTGGTATGT+TGG - chr4_4:47660865-47660884 None:intergenic 30.0%
!!! ATGCCAAATTTTTTGTAAGC+TGG - chr4_4:47661181-47661200 None:intergenic 30.0%
AAAAAATTTGGCATGTGAGT+GGG + chr4_4:47661187-47661206 Msa0684320:CDS 30.0%
!!! ATCTTTTAAGATCTGCAAGA+AGG - chr4_4:47661651-47661670 None:intergenic 30.0%
AGAAGAAATGTCTACAAATC+TGG - chr4_4:47662176-47662195 None:intergenic 30.0%
AAGGGGGTTTATATATGCAT+GGG - chr4_4:47659923-47659942 None:intergenic 35.0%
TAAGGGGGTTTATATATGCA+TGG - chr4_4:47659924-47659943 None:intergenic 35.0%
GGTAGAATCACCAAGTATTA+AGG + chr4_4:47659952-47659971 Msa0684320:five_prime_UTR 35.0%
CACCAAGTATTAAGGAGAAA+TGG + chr4_4:47659960-47659979 Msa0684320:exon 35.0%
! TGCCATTTCTCCTTAATACT+TGG - chr4_4:47659965-47659984 None:intergenic 35.0%
TATTAAGGAGAAATGGCAGA+GGG + chr4_4:47659967-47659986 Msa0684320:exon 35.0%
CAAGTAGCAGAAATAGTAAG+TGG + chr4_4:47659995-47660014 Msa0684320:CDS 35.0%
! ATAGTAAGTGGTCTTTGAAG+GGG + chr4_4:47660007-47660026 Msa0684320:CDS 35.0%
GCACAAAAGAATAAGAGAAG+AGG - chr4_4:47660092-47660111 None:intergenic 35.0%
! AAGCATCATGACTATTGACT+AGG - chr4_4:47660337-47660356 None:intergenic 35.0%
CAATAGTCATGATGCTTGTT+TGG + chr4_4:47660340-47660359 Msa0684320:intron 35.0%
TTACATCAATGGGTCACAAA+AGG + chr4_4:47660602-47660621 Msa0684320:CDS 35.0%
TGAGCAAGATTTCTCATTTC+TGG + chr4_4:47660893-47660912 Msa0684320:CDS 35.0%
!! GAGTTTGAAGCTTTGAGTAA+TGG - chr4_4:47660966-47660985 None:intergenic 35.0%
! TTTTCATGTCCTCCATTGTT+GGG + chr4_4:47660997-47661016 Msa0684320:CDS 35.0%
AGCAAAAGGTAACTGTTTCA+TGG + chr4_4:47661055-47661074 Msa0684320:intron 35.0%
CAAAAAATTTGGCATGTGAG+TGG + chr4_4:47661186-47661205 Msa0684320:CDS 35.0%
AAATTACTTCTGCTGCAAGA+GGG - chr4_4:47661262-47661281 None:intergenic 35.0%
AAAATTACTTCTGCTGCAAG+AGG - chr4_4:47661263-47661282 None:intergenic 35.0%
TGGTATCTGACTAATGAGTT+GGG - chr4_4:47661481-47661500 None:intergenic 35.0%
TTGGTATCTGACTAATGAGT+TGG - chr4_4:47661482-47661501 None:intergenic 35.0%
!! TTAGCCAATTTTCTAGGAGT+TGG - chr4_4:47661530-47661549 None:intergenic 35.0%
! GCAGTATTAGCCAATTTTCT+AGG - chr4_4:47661536-47661555 None:intergenic 35.0%
! AGACCATTTGTATTGATGGA+GGG + chr4_4:47661789-47661808 Msa0684320:CDS 35.0%
TAACCCTCCATCAATACAAA+TGG - chr4_4:47661795-47661814 None:intergenic 35.0%
!! ACAAGTATCGAATACGTATC+TGG - chr4_4:47662282-47662301 None:intergenic 35.0%
ATTCTACCTTCTGGTCTAAG+GGG - chr4_4:47659940-47659959 None:intergenic 40.0%
GATTCTACCTTCTGGTCTAA+GGG - chr4_4:47659941-47659960 None:intergenic 40.0%
TGATTCTACCTTCTGGTCTA+AGG - chr4_4:47659942-47659961 None:intergenic 40.0%
! TACTTGGTGATTCTACCTTC+TGG - chr4_4:47659949-47659968 None:intergenic 40.0%
GTATTAAGGAGAAATGGCAG+AGG + chr4_4:47659966-47659985 Msa0684320:exon 40.0%
AGATAGCAACCCTATTCCTT+TGG - chr4_4:47660058-47660077 None:intergenic 40.0%
AGAGTCTCTTCTGAGTTCAA+TGG + chr4_4:47660689-47660708 Msa0684320:CDS 40.0%
! GTTTTCATGTCCTCCATTGT+TGG + chr4_4:47660996-47661015 Msa0684320:CDS 40.0%
TTTCATGTCCTCCATTGTTG+GGG + chr4_4:47660998-47661017 Msa0684320:CDS 40.0%
ACCATCTATAGTGCAGCAAA+AGG + chr4_4:47661041-47661060 Msa0684320:CDS 40.0%
! ACCTTTTGCTGCACTATAGA+TGG - chr4_4:47661045-47661064 None:intergenic 40.0%
AATTACTTCTGCTGCAAGAG+GGG - chr4_4:47661261-47661280 None:intergenic 40.0%
! TTTCTAGGAGTTGGATGGAT+TGG - chr4_4:47661521-47661540 None:intergenic 40.0%
!! CCAATTTTCTAGGAGTTGGA+TGG - chr4_4:47661526-47661545 None:intergenic 40.0%
CCATCCAACTCCTAGAAAAT+TGG + chr4_4:47661523-47661542 Msa0684320:intron 40.0%
GGACAGACCATTTGTATTGA+TGG + chr4_4:47661785-47661804 Msa0684320:CDS 40.0%
! CAGACCATTTGTATTGATGG+AGG + chr4_4:47661788-47661807 Msa0684320:CDS 40.0%
GTCTCCTTCTACAATAAGCT+AGG + chr4_4:47661825-47661844 Msa0684320:exon 40.0%
CAAGCCTAGCTTATTGTAGA+AGG - chr4_4:47661832-47661851 None:intergenic 40.0%
ATAAACCCCCTTAGACCAGA+AGG + chr4_4:47659931-47659950 Msa0684320:five_prime_UTR 45.0%
TTCTACCTTCTGGTCTAAGG+GGG - chr4_4:47659939-47659958 None:intergenic 45.0%
! ACTGGTGGATCCAAAGGAAT+AGG + chr4_4:47660045-47660064 Msa0684320:CDS 45.0%
! CTGGTGGATCCAAAGGAATA+GGG + chr4_4:47660046-47660065 Msa0684320:CDS 45.0%
TGGAGCAATTAGCAGAGCTA+GGG + chr4_4:47660531-47660550 Msa0684320:CDS 45.0%
!! TGAGTAATGGATGTGCAAGC+TGG - chr4_4:47660953-47660972 None:intergenic 45.0%
TAGTGATGCTACCCCAACAA+TGG - chr4_4:47661012-47661031 None:intergenic 45.0%
GGGGTAGCATCACTAAATGT+AGG + chr4_4:47661017-47661036 Msa0684320:CDS 45.0%
!! AGTGGGCTAGAGACAACATA+AGG + chr4_4:47661204-47661223 Msa0684320:CDS 45.0%
AGGACTAATTGTGTTGCACC+AGG + chr4_4:47661224-47661243 Msa0684320:CDS 45.0%
GGACTAATTGTGTTGCACCA+GGG + chr4_4:47661225-47661244 Msa0684320:CDS 45.0%
! TCGAACTAGTTTGTCCGATG+TGG + chr4_4:47661446-47661465 Msa0684320:intron 45.0%
! CGAACTAGTTTGTCCGATGT+GGG + chr4_4:47661447-47661466 Msa0684320:intron 45.0%
CTCATTTCAAGAACCCCTCT+TGG + chr4_4:47661689-47661708 Msa0684320:CDS 45.0%
TTCTCCAATACGTCCAAGAG+GGG - chr4_4:47661705-47661724 None:intergenic 45.0%
GTTCTCCAATACGTCCAAGA+GGG - chr4_4:47661706-47661725 None:intergenic 45.0%
ACGTATTGGAGAACCAGAAG+AGG + chr4_4:47661712-47661731 Msa0684320:CDS 45.0%
GGTCTGTCCTGTTATGAAAG+AGG - chr4_4:47661774-47661793 None:intergenic 45.0%
TTTGAAGGGGATGACAGCTC+TGG + chr4_4:47660020-47660039 Msa0684320:CDS 50.0%
GTGGAGCAATTAGCAGAGCT+AGG + chr4_4:47660530-47660549 Msa0684320:CDS 50.0%
GGAGCAATTAGCAGAGCTAG+GGG + chr4_4:47660532-47660551 Msa0684320:CDS 50.0%
CCTAGCACAAGTGTGTACAG+TGG - chr4_4:47660558-47660577 None:intergenic 50.0%
CCACTGTACACACTTGTGCT+AGG + chr4_4:47660555-47660574 Msa0684320:CDS 50.0%
GTCATCTCGTGCACAAAGAG+AGG + chr4_4:47660655-47660674 Msa0684320:CDS 50.0%
TGATGCTACCCCAACAATGG+AGG - chr4_4:47661009-47661028 None:intergenic 50.0%
!! TTGGGCTAGTCATCCCACAT+CGG - chr4_4:47661463-47661482 None:intergenic 50.0%
AGAACCCCTCTTGGACGTAT+TGG + chr4_4:47661698-47661717 Msa0684320:CDS 50.0%
GGTTCTCCAATACGTCCAAG+AGG - chr4_4:47661707-47661726 None:intergenic 50.0%
!! ACCAGAAGAGGTGTCTTCCT+TGG + chr4_4:47661724-47661743 Msa0684320:CDS 50.0%
ACCAAGGAAGACACCTCTTC+TGG - chr4_4:47661728-47661747 None:intergenic 50.0%
!! AGAAGAGGTGTCTTCCTTGG+TGG + chr4_4:47661727-47661746 Msa0684320:CDS 50.0%
TAAGCAGAGGAATGCCACCA+AGG - chr4_4:47661744-47661763 None:intergenic 50.0%
CCTGTTATGAAAGAGGCTGC+AGG - chr4_4:47661767-47661786 None:intergenic 50.0%
CCTGCAGCCTCTTTCATAAC+AGG + chr4_4:47661764-47661783 Msa0684320:CDS 50.0%
! ATGACAGCTCTGGTCACTGG+TGG + chr4_4:47660030-47660049 Msa0684320:CDS 55.0%
! CTGGTCACTGGTGGATCCAA+AGG + chr4_4:47660039-47660058 Msa0684320:CDS 55.0%
! GCTGCAAGAGGGGTTCTGAT+TGG - chr4_4:47661251-47661270 None:intergenic 55.0%
AAGAGGCTGCAGGTAAGCAG+AGG - chr4_4:47661757-47661776 None:intergenic 55.0%
! GGGATGACAGCTCTGGTCAC+TGG + chr4_4:47660027-47660046 Msa0684320:CDS 60.0%
! AGAGGGGTTCTGATTGGCCC+TGG - chr4_4:47661245-47661264 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 47659812 47662302 47659812 ID=Msa0684320;Name=Msa0684320
chr4_4 mRNA 47659812 47662302 47659812 ID=Msa0684320-mRNA-1;Parent=Msa0684320;Name=Msa0684320-mRNA-1;_AED=0.06;_eAED=0.06;_QI=167|1|1|1|1|1|5|461|267
chr4_4 exon 47659812 47660067 47659812 ID=Msa0684320-mRNA-1:exon:11747;Parent=Msa0684320-mRNA-1
chr4_4 exon 47660520 47660727 47660520 ID=Msa0684320-mRNA-1:exon:11748;Parent=Msa0684320-mRNA-1
chr4_4 exon 47660846 47661062 47660846 ID=Msa0684320-mRNA-1:exon:11749;Parent=Msa0684320-mRNA-1
chr4_4 exon 47661168 47661274 47661168 ID=Msa0684320-mRNA-1:exon:11750;Parent=Msa0684320-mRNA-1
chr4_4 exon 47661659 47662302 47661659 ID=Msa0684320-mRNA-1:exon:11751;Parent=Msa0684320-mRNA-1
chr4_4 five_prime_UTR 47659812 47659978 47659812 ID=Msa0684320-mRNA-1:five_prime_utr;Parent=Msa0684320-mRNA-1
chr4_4 CDS 47659979 47660067 47659979 ID=Msa0684320-mRNA-1:cds;Parent=Msa0684320-mRNA-1
chr4_4 CDS 47660520 47660727 47660520 ID=Msa0684320-mRNA-1:cds;Parent=Msa0684320-mRNA-1
chr4_4 CDS 47660846 47661062 47660846 ID=Msa0684320-mRNA-1:cds;Parent=Msa0684320-mRNA-1
chr4_4 CDS 47661168 47661274 47661168 ID=Msa0684320-mRNA-1:cds;Parent=Msa0684320-mRNA-1
chr4_4 CDS 47661659 47661841 47661659 ID=Msa0684320-mRNA-1:cds;Parent=Msa0684320-mRNA-1
chr4_4 three_prime_UTR 47661842 47662302 47661842 ID=Msa0684320-mRNA-1:three_prime_utr;Parent=Msa0684320-mRNA-1
Gene Sequence

>Msa0684320

ATGGCAGAGGGAAACACAAGTAGCAGAAATAGTAAGTGGTCTTTGAAGGGGATGACAGCTCTGGTCACTGGTGGATCCAAAGGAATAGGGTATGATATAGTGGAGCAATTAGCAGAGCTAGGGGCCACTGTACACACTTGTGCTAGGAATGAAGCTAAACTCAATGAATGCTTACATCAATGGGTCACAAAAGGATACAAAATCACTGGTTCAGTCTGTGATGTGTCATCTCGTGCACAAAGAGAGGAGCTCATAGCTAGAGTCTCTTCTGAGTTCAATGGCAAACTCAATATCCTTGTGAACAATGTGGGAACCAACATACCAAAACAATCACTAGATTTTACTGAGCAAGATTTCTCATTTCTGGTGAATACAAATCTTGAATCTGCTTTTCACATAAGCCAGCTTGCACATCCATTACTCAAAGCTTCAAACTCTGCAAGCATTGTTTTCATGTCCTCCATTGTTGGGGTAGCATCACTAAATGTAGGAACCATCTATAGTGCAGCAAAAGGAGCAATAAACCAGCTTACAAAAAATTTGGCATGTGAGTGGGCTAGAGACAACATAAGGACTAATTGTGTTGCACCAGGGCCAATCAGAACCCCTCTTGCAGCAGAAATCTTAAAAGATGAAAAACTTCTCGATGCTCTCATTTCAAGAACCCCTCTTGGACGTATTGGAGAACCAGAAGAGGTGTCTTCCTTGGTGGCATTCCTCTGCTTACCTGCAGCCTCTTTCATAACAGGACAGACCATTTGTATTGATGGAGGGTTAACTGTCAACTGTCTCCTTCTACAATAA
Protein sequence

>Msa0684320

MAEGNTSSRNSKWSLKGMTALVTGGSKGIGYDIVEQLAELGATVHTCARNEAKLNECLHQWVTKGYKITGSVCDVSSRAQREELIARVSSEFNGKLNILVNNVGTNIPKQSLDFTEQDFSFLVNTNLESAFHISQLAHPLLKASNSASIVFMSSIVGVASLNVGTIYSAAKGAINQLTKNLACEWARDNIRTNCVAPGPIRTPLAAEILKDEKLLDALISRTPLGRIGEPEEVSSLVAFLCLPAASFITGQTICIDGGLTVNCLLLQ*