AlfalfaGEDB | Msa0663950

M. sativa cultivar zhongmu-4 / Msa0663950

KEGG: K15032
GO: -
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663950	XP_013454526.1	95.526	380	10	1	1	380	1	373	0.0	737

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663950	A0A072UFU8	95.526	380	10	1	1	380	1	373	0.0	737

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TFs/TRs:

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Gene ID	Type	Classification
Msa0663950	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0626070	Msa0663950	0.844557	7.020873e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663950	MtrunA17_Chr4g0001831	95.526	380	10	1	1	380	1	373	0.0	737
Msa0663950	MtrunA17_Chr2g0286191	37.158	366	218	5	14	378	6	360	1.41e-68	220
Msa0663950	MtrunA17_Chr4g0067121	34.835	333	209	5	45	375	52	378	1.01e-58	195
Msa0663950	MtrunA17_Chr4g0067671	32.641	337	213	5	27	360	30	355	1.83e-57	191
Msa0663950	MtrunA17_Chr2g0296301	29.851	335	226	3	43	374	35	363	2.02e-53	181
Msa0663950	MtrunA17_Chr2g0296121	33.148	359	226	7	22	375	105	454	3.98e-53	182
Msa0663950	MtrunA17_Chr4g0067141	32.877	365	225	6	22	379	23	374	6.72e-53	179
Msa0663950	MtrunA17_Chr2g0295761	33.526	346	210	6	36	374	54	386	3.88e-52	178
Msa0663950	MtrunA17_Chr2g0295731	30.939	362	240	6	17	375	21	375	6.53e-50	172
Msa0663950	MtrunA17_Chr2g0296151	30.811	370	245	6	11	375	5	368	6.77e-49	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663950	AT5G07900.1	41.176	340	197	3	37	374	61	399	2.67e-88	273
Msa0663950	AT1G21150.1	33.693	371	239	4	10	374	17	386	2.69e-61	202
Msa0663950	AT1G21150.2	33.693	371	239	4	10	374	81	450	1.20e-60	202
Msa0663950	AT1G21150.3	34.000	350	222	4	33	374	2	350	2.45e-59	196
Msa0663950	AT1G62120.1	30.179	391	226	7	14	360	18	405	3.77e-43	155
Msa0663950	AT1G61980.3	29.412	357	210	5	41	360	48	399	9.57e-40	146
Msa0663950	AT1G61980.2	29.412	357	210	5	41	360	48	399	9.57e-40	146
Msa0663950	AT1G61980.1	29.412	357	210	5	41	360	48	399	9.57e-40	146
Msa0663950	AT1G61970.2	28.650	363	207	7	41	361	48	400	1.02e-39	146
Msa0663950	AT1G61970.7	28.650	363	207	7	41	361	48	400	1.02e-39	146

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Find 66 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAATACAACTTCGA+AGG	0.568698	4_4:-2874945	None:intergenic
AAAAGCTGAATAACAGCATT+TGG	0.312391	4_4:-2874474	None:intergenic
AAAAGGTAGTTCTAGCTTTG+AGG	0.445792	4_4:+2874733	Msa0663950:CDS
AAACTGGAAATAGAACTTTG+AGG	0.609479	4_4:-2874837	None:intergenic
AAAGAACAGTAGTTGAATAA+AGG	0.305931	4_4:-2874290	None:intergenic
AAAGCTGAATAACAGCATTT+GGG	0.472696	4_4:-2874473	None:intergenic
AACAATGGACTTCTTGGTGA+AGG	0.587900	4_4:+2875118	Msa0663950:CDS
AAGAACAGTAGTTGAATAAA+GGG	0.427774	4_4:-2874289	None:intergenic
AAGACATTGCTACATGCCCT+GGG	0.601471	4_4:+2875159	Msa0663950:CDS
AAGCCCTTAAACTTTCAACA+AGG	0.642886	4_4:+2874427	Msa0663950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCCAACACAAATCAACATAA+AGG	+	chr4_4:2874351-2874370	Msa0663950:CDS	30.0%
TATGAGGTTTGACAATGTAA+AGG	-	chr4_4:2874382-2874401	None:intergenic	30.0%
CAACCTTGTTGAAAGTTTAA+GGG	-	chr4_4:2874433-2874452	None:intergenic	30.0%
AAAGCTGAATAACAGCATTT+GGG	-	chr4_4:2874476-2874495	None:intergenic	30.0%
AAAAGCTGAATAACAGCATT+TGG	-	chr4_4:2874477-2874496	None:intergenic	30.0%
AAACTGGAAATAGAACTTTG+AGG	-	chr4_4:2874840-2874859	None:intergenic	30.0%
TCAAAGTTCTATTTCCAGTT+TGG	+	chr4_4:2874839-2874858	Msa0663950:CDS	30.0%
GATTCGAGTAATTGTAAGTA+TGG	+	chr4_4:2874971-2874990	Msa0663950:CDS	30.0%
TCAAGAGGTTTAATAAAGAG+TGG	+	chr4_4:2875245-2875264	Msa0663950:CDS	30.0%
TACCTTTATTATGACAAGTG+AGG	+	chr4_4:2875280-2875299	Msa0663950:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	2874246	2875388	2874246	ID=Msa0663950;Name=Msa0663950
chr4_4	mRNA	2874246	2875388	2874246	ID=Msa0663950-mRNA-1;Parent=Msa0663950;Name=Msa0663950-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|380
chr4_4	exon	2874246	2875388	2874246	ID=Msa0663950-mRNA-1:exon:819;Parent=Msa0663950-mRNA-1
chr4_4	CDS	2874246	2875388	2874246	ID=Msa0663950-mRNA-1:cds;Parent=Msa0663950-mRNA-1

Gene Sequence

>Msa0663950

ATGTATCATTTTCTCATTGCTAGATTCACTCATACTCATAGAACCCTTTATTCAACTACTGTTCTTTATCACAGACACAATCCTTTTTCACTTTTCTCTGCTGTTTCCAACACAAATCAACATAAAGGTGACACCTTTACATTGTCAAACCTCATAAACTCATGTGGGTTGTCTCCTGAAAAAGCCCTTAAACTTTCAACAAGGTTGCAACTCATAAACCCTAATGGCCCAAATGCTGTTATTCAGCTTTTCAGAAGCTATGGTTTCTCTGATTCTCAGTTATCAAGCCTTGTTAAGAAACACCCTTTTGTACTTTTATCAAAATCTGATAAGACCCTTTTGCCAAAACTCAAGTTTTTGCAATCCATTGGGGCTTCAACCATTGATTTCCCCAAAATCTTGATTGGTGACAAGTTTTTGACAGCAAGCTTGGAGAAAACGATAATACCTCGTTACAAGATTCTTAAAAGCCTAGTTTGCGATGATAAAAAGGTAGTTCTAGCTTTGAGGCGTGGATCATGGAACTTTTACAATGATTCCATGGTTAATGATTCTGTTCCGAACATTGAGGTTTTGAGGAAGCTTGGTGTGCCTCAAAGTTCTATTTCCAGTTTGGTATCCAATTTTCCTTGTGTAGCATTCACCAAGCATTCTAGATTTGTTGAGGCCGTTAATTCAGTAAAAGAAATGGGGTTCAATCCTTCGAAGTTGTATTTTGTTTTGGCGATTCGAGTAATTGTAAGTATGGACAAAGAAATTTGGGAATCAAAATTTAAGATTTTCGAGAGGTGGGGCTGGTCGAGAGATATTTGTCGTTTCGCTTTCCTAAAGTATCCTGAGTATGTGATGATCTCAGAGAAAAAAATTATGAAAACAATGGACTTCTTGGTGAAGGATGTGGGTCTTACACCAGAAGACATTGCTACATGCCCTGGGATTCTGACCCGCAACTTGGAGAAGACACTTGTACCTAAATGTGCTGTTATCAAGATTTTGAAGTCAAGAGGTTTAATAAAGAGTGGTTTGCGTGTAAGTACCTTTATTATGACAAGTGAGGAAAAGTTTGTGCAGAAATATGTGACCCCATTTCAGAAAGATTTGCCATTGTTATTGGATGCATATAAAGTTCAGAAATCAGATTGA

Protein sequence

>Msa0663950

MYHFLIARFTHTHRTLYSTTVLYHRHNPFSLFSAVSNTNQHKGDTFTLSNLINSCGLSPEKALKLSTRLQLINPNGPNAVIQLFRSYGFSDSQLSSLVKKHPFVLLSKSDKTLLPKLKFLQSIGASTIDFPKILIGDKFLTASLEKTIIPRYKILKSLVCDDKKVVLALRRGSWNFYNDSMVNDSVPNIEVLRKLGVPQSSISSLVSNFPCVAFTKHSRFVEAVNSVKEMGFNPSKLYFVLAIRVIVSMDKEIWESKFKIFERWGWSRDICRFAFLKYPEYVMISEKKIMKTMDFLVKDVGLTPEDIATCPGILTRNLEKTLVPKCAVIKILKSRGLIKSGLRVSTFIMTSEEKFVQKYVTPFQKDLPLLLDAYKVQKSD*