Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665260 | XP_039689884.1 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 1.11e-74 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665260 | sp|Q1G3V9|EPFL8_ARATH | 31.776 | 107 | 62 | 3 | 5 | 111 | 4 | 99 | 6.82e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665260 | G7JGZ2 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 5.31e-75 | 226 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0530540 | Msa0665260 | 0.869604 | 2.734231e-66 | -8.615850e-47 |
| Msa0587410 | Msa0665260 | 0.867435 | 1.371997e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665260 | MtrunA17_Chr2g0333071 | 53.398 | 103 | 37 | 3 | 19 | 111 | 12 | 113 | 4.42e-32 | 108 |
| Msa0665260 | MtrunA17_Chr4g0063871 | 41.284 | 109 | 57 | 2 | 10 | 111 | 6 | 114 | 2.20e-26 | 94.4 |
| Msa0665260 | MtrunA17_Chr4g0019101 | 34.043 | 94 | 47 | 3 | 23 | 111 | 79 | 162 | 7.93e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0665260 | AT1G80133.1 | 31.776 | 107 | 62 | 3 | 5 | 111 | 4 | 99 | 6.94e-12 | 57.4 |
Find 25 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACGCTAATTTCCAAATC+AGG | 0.327725 | 4_4:+4631564 | Msa0665260:CDS |
| TAAATAAGTGCATGAATTGT+AGG | 0.332110 | 4_4:+4632469 | Msa0665260:CDS |
| GAAATGTAGATGTGGGAATA+AGG | 0.373779 | 4_4:+4632587 | Msa0665260:CDS |
| GAAATGATAGCTTGGAAGAC+AGG | 0.390549 | 4_4:+4632412 | Msa0665260:CDS |
| GAAGACAGGAAAATGGTGAT+AGG | 0.411712 | 4_4:+4632426 | Msa0665260:CDS |
| TAGCTTGGAAGACAGGAAAA+TGG | 0.419909 | 4_4:+4632419 | Msa0665260:CDS |
| TAGGCCTTGTATAGCAACTT+TGG | 0.443073 | 4_4:+4632488 | Msa0665260:CDS |
| ATCTACATTTCCATGCTAGA+AGG | 0.450414 | 4_4:-4632575 | None:intergenic |
| TTTGAGTCCATGTAGATGAT+AGG | 0.456363 | 4_4:-4631502 | None:intergenic |
| ATGCACTTATTTAAACAAGC+AGG | 0.501769 | 4_4:-4632459 | None:intergenic |
| ATACTTATTACCTTCTAGCA+TGG | 0.506026 | 4_4:+4632565 | Msa0665260:CDS |
| TGCTTGAGCAGGTGGATCAT+TGG | 0.518289 | 4_4:+4632380 | Msa0665260:intron |
| AGCAGGTGGATCATTGGTGT+TGG | 0.551304 | 4_4:+4632386 | Msa0665260:intron |
| GTTGGAGAGAAATGATAGCT+TGG | 0.558825 | 4_4:+4632404 | Msa0665260:CDS |
| TGTAGATGATAGGATCTAGC+AGG | 0.568991 | 4_4:-4631492 | None:intergenic |
| GTTACAAAGTGTTATCTCGT+GGG | 0.569596 | 4_4:+4632532 | Msa0665260:CDS |
| AAGACAGGAAAATGGTGATA+GGG | 0.574619 | 4_4:+4632427 | Msa0665260:CDS |
| GCTAGATCCTATCATCTACA+TGG | 0.581934 | 4_4:+4631495 | Msa0665260:CDS |
| AGTTACAAAGTGTTATCTCG+TGG | 0.590051 | 4_4:+4632531 | Msa0665260:CDS |
| CTAGCATGGAAATGTAGATG+TGG | 0.600363 | 4_4:+4632579 | Msa0665260:CDS |
| CACTTATTTAAACAAGCAGG+AGG | 0.601716 | 4_4:-4632456 | None:intergenic |
| TAGCATGGAAATGTAGATGT+GGG | 0.603897 | 4_4:+4632580 | Msa0665260:CDS |
| ACAACCAAAGTTGCTATACA+AGG | 0.612351 | 4_4:-4632492 | None:intergenic |
| TTACAAAGTGTTATCTCGTG+GGG | 0.657713 | 4_4:+4632533 | Msa0665260:CDS |
| AGATGATAGGATCTAGCAGG+TGG | 0.672567 | 4_4:-4631489 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAATGAAAATAACTATT+TGG | - | chr4_4:4631675-4631694 | None:intergenic | 15.0% |
| !!! | AAGTTTAGATTTTAAAACAA+AGG | - | chr4_4:4631737-4631756 | None:intergenic | 15.0% |
| !! | CTTATTATTCTTCTATTATT+AGG | + | chr4_4:4631763-4631782 | Msa0665260:intron | 15.0% |
| !!! | AAAAACTCTAAAATAAATGA+TGG | + | chr4_4:4632188-4632207 | Msa0665260:intron | 15.0% |
| !! | TATGAATGAAAATAACTATT+TGG | - | chr4_4:4631675-4631694 | None:intergenic | 15.0% |
| !!! | AAGTTTAGATTTTAAAACAA+AGG | - | chr4_4:4631737-4631756 | None:intergenic | 15.0% |
| !! | CTTATTATTCTTCTATTATT+AGG | + | chr4_4:4631763-4631782 | Msa0665260:intron | 15.0% |
| !!! | AAAAACTCTAAAATAAATGA+TGG | + | chr4_4:4632188-4632207 | Msa0665260:intron | 15.0% |
| !! | TAGTATATACAAACCAAAAT+AGG | - | chr4_4:4631831-4631850 | None:intergenic | 20.0% |
| !! | AAAAGTTTATTTATCTGCAT+AGG | - | chr4_4:4631941-4631960 | None:intergenic | 20.0% |
| !! | TAGTATATACAAACCAAAAT+AGG | - | chr4_4:4631831-4631850 | None:intergenic | 20.0% |
| !! | AAAAGTTTATTTATCTGCAT+AGG | - | chr4_4:4631941-4631960 | None:intergenic | 20.0% |
| !!! | AGCTTTTTAATTACCTGATT+TGG | - | chr4_4:4631580-4631599 | None:intergenic | 25.0% |
| !! | CATCATTCATTTCTTTTGTT+TGG | + | chr4_4:4631702-4631721 | Msa0665260:intron | 25.0% |
| !! | ATAATTGTCAACTCCTATTT+TGG | + | chr4_4:4631815-4631834 | Msa0665260:intron | 25.0% |
| ! | AAAAATAAGTCAATCCAAAC+AGG | + | chr4_4:4632134-4632153 | Msa0665260:intron | 25.0% |
| !!! | TGTTTTTAATGTGTGTGAAA+TGG | + | chr4_4:4632266-4632285 | Msa0665260:intron | 25.0% |
| ! | TAAATAAGTGCATGAATTGT+AGG | + | chr4_4:4632469-4632488 | Msa0665260:CDS | 25.0% |
| !!! | AGCTTTTTAATTACCTGATT+TGG | - | chr4_4:4631580-4631599 | None:intergenic | 25.0% |
| !! | CATCATTCATTTCTTTTGTT+TGG | + | chr4_4:4631702-4631721 | Msa0665260:intron | 25.0% |
| !! | ATAATTGTCAACTCCTATTT+TGG | + | chr4_4:4631815-4631834 | Msa0665260:intron | 25.0% |
| ! | AAAAATAAGTCAATCCAAAC+AGG | + | chr4_4:4632134-4632153 | Msa0665260:intron | 25.0% |
| !!! | TGTTTTTAATGTGTGTGAAA+TGG | + | chr4_4:4632266-4632285 | Msa0665260:intron | 25.0% |
| ! | TAAATAAGTGCATGAATTGT+AGG | + | chr4_4:4632469-4632488 | Msa0665260:CDS | 25.0% |
| !!! | CAGTTTTGTTTAGCTTGAAT+AGG | - | chr4_4:4631618-4631637 | None:intergenic | 30.0% |
| ATGCACTTATTTAAACAAGC+AGG | - | chr4_4:4632462-4632481 | None:intergenic | 30.0% | |
| ATACTTATTACCTTCTAGCA+TGG | + | chr4_4:4632565-4632584 | Msa0665260:CDS | 30.0% | |
| !!! | CAGTTTTGTTTAGCTTGAAT+AGG | - | chr4_4:4631618-4631637 | None:intergenic | 30.0% |
| ATGCACTTATTTAAACAAGC+AGG | - | chr4_4:4632462-4632481 | None:intergenic | 30.0% | |
| ATACTTATTACCTTCTAGCA+TGG | + | chr4_4:4632565-4632584 | Msa0665260:CDS | 30.0% | |
| TTTGAGTCCATGTAGATGAT+AGG | - | chr4_4:4631505-4631524 | None:intergenic | 35.0% | |
| !! | GTTTAGCTTGAATAGGTATG+AGG | - | chr4_4:4631611-4631630 | None:intergenic | 35.0% |
| AAGACAGGAAAATGGTGATA+GGG | + | chr4_4:4632427-4632446 | Msa0665260:CDS | 35.0% | |
| CACTTATTTAAACAAGCAGG+AGG | - | chr4_4:4632459-4632478 | None:intergenic | 35.0% | |
| ACAACCAAAGTTGCTATACA+AGG | - | chr4_4:4632495-4632514 | None:intergenic | 35.0% | |
| ! | AGTTACAAAGTGTTATCTCG+TGG | + | chr4_4:4632531-4632550 | Msa0665260:CDS | 35.0% |
| ! | GTTACAAAGTGTTATCTCGT+GGG | + | chr4_4:4632532-4632551 | Msa0665260:CDS | 35.0% |
| ! | TTACAAAGTGTTATCTCGTG+GGG | + | chr4_4:4632533-4632552 | Msa0665260:CDS | 35.0% |
| ATCTACATTTCCATGCTAGA+AGG | - | chr4_4:4632578-4632597 | None:intergenic | 35.0% | |
| TAGCATGGAAATGTAGATGT+GGG | + | chr4_4:4632580-4632599 | Msa0665260:CDS | 35.0% | |
| GAAATGTAGATGTGGGAATA+AGG | + | chr4_4:4632587-4632606 | Msa0665260:CDS | 35.0% | |
| TTTGAGTCCATGTAGATGAT+AGG | - | chr4_4:4631505-4631524 | None:intergenic | 35.0% | |
| !! | GTTTAGCTTGAATAGGTATG+AGG | - | chr4_4:4631611-4631630 | None:intergenic | 35.0% |
| AAGACAGGAAAATGGTGATA+GGG | + | chr4_4:4632427-4632446 | Msa0665260:CDS | 35.0% | |
| CACTTATTTAAACAAGCAGG+AGG | - | chr4_4:4632459-4632478 | None:intergenic | 35.0% | |
| ACAACCAAAGTTGCTATACA+AGG | - | chr4_4:4632495-4632514 | None:intergenic | 35.0% | |
| ! | AGTTACAAAGTGTTATCTCG+TGG | + | chr4_4:4632531-4632550 | Msa0665260:CDS | 35.0% |
| ! | GTTACAAAGTGTTATCTCGT+GGG | + | chr4_4:4632532-4632551 | Msa0665260:CDS | 35.0% |
| ! | TTACAAAGTGTTATCTCGTG+GGG | + | chr4_4:4632533-4632552 | Msa0665260:CDS | 35.0% |
| ATCTACATTTCCATGCTAGA+AGG | - | chr4_4:4632578-4632597 | None:intergenic | 35.0% | |
| TAGCATGGAAATGTAGATGT+GGG | + | chr4_4:4632580-4632599 | Msa0665260:CDS | 35.0% | |
| GAAATGTAGATGTGGGAATA+AGG | + | chr4_4:4632587-4632606 | Msa0665260:CDS | 35.0% | |
| TGTAGATGATAGGATCTAGC+AGG | - | chr4_4:4631495-4631514 | None:intergenic | 40.0% | |
| GCTAGATCCTATCATCTACA+TGG | + | chr4_4:4631495-4631514 | Msa0665260:CDS | 40.0% | |
| TGCACGCTAATTTCCAAATC+AGG | + | chr4_4:4631564-4631583 | Msa0665260:CDS | 40.0% | |
| TTGAATAGGTATGAGGTTGG+TGG | - | chr4_4:4631604-4631623 | None:intergenic | 40.0% | |
| AGCTTGAATAGGTATGAGGT+TGG | - | chr4_4:4631607-4631626 | None:intergenic | 40.0% | |
| TTATTGACCTAGGACCTGTT+TGG | - | chr4_4:4632151-4632170 | None:intergenic | 40.0% | |
| !! | CATGTGTCACTTATTGACCT+AGG | - | chr4_4:4632161-4632180 | None:intergenic | 40.0% |
| GAATGTGTATATGCTTGAGC+AGG | + | chr4_4:4632369-4632388 | Msa0665260:intron | 40.0% | |
| GTTGGAGAGAAATGATAGCT+TGG | + | chr4_4:4632404-4632423 | Msa0665260:CDS | 40.0% | |
| GAAATGATAGCTTGGAAGAC+AGG | + | chr4_4:4632412-4632431 | Msa0665260:CDS | 40.0% | |
| TAGCTTGGAAGACAGGAAAA+TGG | + | chr4_4:4632419-4632438 | Msa0665260:CDS | 40.0% | |
| GAAGACAGGAAAATGGTGAT+AGG | + | chr4_4:4632426-4632445 | Msa0665260:CDS | 40.0% | |
| ! | TAGGCCTTGTATAGCAACTT+TGG | + | chr4_4:4632488-4632507 | Msa0665260:CDS | 40.0% |
| CTAGCATGGAAATGTAGATG+TGG | + | chr4_4:4632579-4632598 | Msa0665260:CDS | 40.0% | |
| TGTAGATGATAGGATCTAGC+AGG | - | chr4_4:4631495-4631514 | None:intergenic | 40.0% | |
| GCTAGATCCTATCATCTACA+TGG | + | chr4_4:4631495-4631514 | Msa0665260:CDS | 40.0% | |
| TGCACGCTAATTTCCAAATC+AGG | + | chr4_4:4631564-4631583 | Msa0665260:CDS | 40.0% | |
| TTGAATAGGTATGAGGTTGG+TGG | - | chr4_4:4631604-4631623 | None:intergenic | 40.0% | |
| AGCTTGAATAGGTATGAGGT+TGG | - | chr4_4:4631607-4631626 | None:intergenic | 40.0% | |
| TTATTGACCTAGGACCTGTT+TGG | - | chr4_4:4632151-4632170 | None:intergenic | 40.0% | |
| !! | CATGTGTCACTTATTGACCT+AGG | - | chr4_4:4632161-4632180 | None:intergenic | 40.0% |
| GAATGTGTATATGCTTGAGC+AGG | + | chr4_4:4632369-4632388 | Msa0665260:intron | 40.0% | |
| GTTGGAGAGAAATGATAGCT+TGG | + | chr4_4:4632404-4632423 | Msa0665260:CDS | 40.0% | |
| GAAATGATAGCTTGGAAGAC+AGG | + | chr4_4:4632412-4632431 | Msa0665260:CDS | 40.0% | |
| TAGCTTGGAAGACAGGAAAA+TGG | + | chr4_4:4632419-4632438 | Msa0665260:CDS | 40.0% | |
| GAAGACAGGAAAATGGTGAT+AGG | + | chr4_4:4632426-4632445 | Msa0665260:CDS | 40.0% | |
| ! | TAGGCCTTGTATAGCAACTT+TGG | + | chr4_4:4632488-4632507 | Msa0665260:CDS | 40.0% |
| CTAGCATGGAAATGTAGATG+TGG | + | chr4_4:4632579-4632598 | Msa0665260:CDS | 40.0% | |
| AGATGATAGGATCTAGCAGG+TGG | - | chr4_4:4631492-4631511 | None:intergenic | 45.0% | |
| AGTCAATCCAAACAGGTCCT+AGG | + | chr4_4:4632141-4632160 | Msa0665260:intron | 45.0% | |
| TGTGTATATGCTTGAGCAGG+TGG | + | chr4_4:4632372-4632391 | Msa0665260:intron | 45.0% | |
| AGATGATAGGATCTAGCAGG+TGG | - | chr4_4:4631492-4631511 | None:intergenic | 45.0% | |
| AGTCAATCCAAACAGGTCCT+AGG | + | chr4_4:4632141-4632160 | Msa0665260:intron | 45.0% | |
| TGTGTATATGCTTGAGCAGG+TGG | + | chr4_4:4632372-4632391 | Msa0665260:intron | 45.0% | |
| TGCTTGAGCAGGTGGATCAT+TGG | + | chr4_4:4632380-4632399 | Msa0665260:intron | 50.0% | |
| AGCAGGTGGATCATTGGTGT+TGG | + | chr4_4:4632386-4632405 | Msa0665260:intron | 50.0% | |
| TGCTTGAGCAGGTGGATCAT+TGG | + | chr4_4:4632380-4632399 | Msa0665260:intron | 50.0% | |
| AGCAGGTGGATCATTGGTGT+TGG | + | chr4_4:4632386-4632405 | Msa0665260:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 4631483 | 4632623 | 4631483 | ID=Msa0665260;Name=Msa0665260 |
| chr4_4 | mRNA | 4631483 | 4632623 | 4631483 | ID=Msa0665260-mRNA-1;Parent=Msa0665260;Name=Msa0665260-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|111 |
| chr4_4 | exon | 4631483 | 4631585 | 4631483 | ID=Msa0665260-mRNA-1:exon:1544;Parent=Msa0665260-mRNA-1 |
| chr4_4 | exon | 4632391 | 4632623 | 4632391 | ID=Msa0665260-mRNA-1:exon:1545;Parent=Msa0665260-mRNA-1 |
| chr4_4 | CDS | 4631483 | 4631585 | 4631483 | ID=Msa0665260-mRNA-1:cds;Parent=Msa0665260-mRNA-1 |
| chr4_4 | CDS | 4632391 | 4632623 | 4632391 | ID=Msa0665260-mRNA-1:cds;Parent=Msa0665260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
