Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0587410 | XP_039689884.1 | 98.198 | 111 | 2 | 0 | 1 | 111 | 1 | 111 | 1.66e-75 | 228 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0587410 | sp|Q1G3V9|EPFL8_ARATH | 31.776 | 107 | 62 | 3 | 5 | 111 | 4 | 99 | 4.68e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0024550 | Msa0587410 | 0.809628 | 1.722658e-50 | -8.615850e-47 |
| Msa0120400 | Msa0587410 | 0.809809 | 1.574545e-50 | -8.615850e-47 |
| Msa0530540 | Msa0587410 | 0.978310 | 1.472121e-145 | -8.615850e-47 |
| Msa0587410 | Msa0665260 | 0.867435 | 1.371997e-65 | -8.615850e-47 |
| Msa0587410 | Msa0967380 | 0.820832 | 5.570203e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0587410 | MtrunA17_Chr2g0333071 | 53.398 | 103 | 37 | 3 | 19 | 111 | 12 | 113 | 6.20e-32 | 108 |
| Msa0587410 | MtrunA17_Chr4g0063871 | 41.284 | 109 | 57 | 2 | 10 | 111 | 6 | 114 | 2.89e-26 | 94.4 |
| Msa0587410 | MtrunA17_Chr4g0019101 | 34.043 | 94 | 47 | 3 | 23 | 111 | 79 | 162 | 2.14e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0587410 | AT1G80133.1 | 31.776 | 107 | 62 | 3 | 5 | 111 | 4 | 99 | 4.75e-12 | 57.8 |
Find 24 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAATAAGTGCATGAATTGT+AGG | 0.332110 | 4_2:+8902182 | Msa0587410:CDS |
| GAAATGTAGATGTGGGAATA+AGG | 0.373779 | 4_2:+8902300 | Msa0587410:CDS |
| GAAATGATAGCTTGGAAGAC+AGG | 0.390549 | 4_2:+8902125 | Msa0587410:CDS |
| GAAGACAGGAAAATGGTGAT+AGG | 0.411712 | 4_2:+8902139 | Msa0587410:CDS |
| TAGCTTGGAAGACAGGAAAA+TGG | 0.419909 | 4_2:+8902132 | Msa0587410:CDS |
| TAGGCCTTGTATAGCAACTT+TGG | 0.443073 | 4_2:+8902201 | Msa0587410:CDS |
| ATCTACATTTCCATGCTAGA+AGG | 0.450414 | 4_2:-8902288 | None:intergenic |
| TTTGAGTCCATGTAGATGAT+AGG | 0.456363 | 4_2:-8901214 | None:intergenic |
| ATGCACTTATTTAAACAAGC+AGG | 0.501769 | 4_2:-8902172 | None:intergenic |
| ATACTTATTACCTTCTAGCA+TGG | 0.506026 | 4_2:+8902278 | Msa0587410:CDS |
| TGCTTGAGCAGGTGGATCAT+TGG | 0.518289 | 4_2:+8902093 | Msa0587410:intron |
| AGCAGGTGGATCATTGGTGT+TGG | 0.551304 | 4_2:+8902099 | Msa0587410:intron |
| GTTGGAGAGAAATGATAGCT+TGG | 0.558825 | 4_2:+8902117 | Msa0587410:CDS |
| TGTAGATGATAGGATCTAGC+AGG | 0.568991 | 4_2:-8901204 | None:intergenic |
| GTTACAAAGTGTTATCTCGT+GGG | 0.569596 | 4_2:+8902245 | Msa0587410:CDS |
| AAGACAGGAAAATGGTGATA+GGG | 0.574619 | 4_2:+8902140 | Msa0587410:CDS |
| GCTAGATCCTATCATCTACA+TGG | 0.581934 | 4_2:+8901207 | Msa0587410:CDS |
| AGTTACAAAGTGTTATCTCG+TGG | 0.590051 | 4_2:+8902244 | Msa0587410:CDS |
| CTAGCATGGAAATGTAGATG+TGG | 0.600363 | 4_2:+8902292 | Msa0587410:CDS |
| CACTTATTTAAACAAGCAGG+AGG | 0.601716 | 4_2:-8902169 | None:intergenic |
| TAGCATGGAAATGTAGATGT+GGG | 0.603897 | 4_2:+8902293 | Msa0587410:CDS |
| ACAACCAAAGTTGCTATACA+AGG | 0.612351 | 4_2:-8902205 | None:intergenic |
| TTACAAAGTGTTATCTCGTG+GGG | 0.657713 | 4_2:+8902246 | Msa0587410:CDS |
| AGATGATAGGATCTAGCAGG+TGG | 0.672567 | 4_2:-8901201 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGAATGAAAATAACTATT+TGG | - | chr4_2:8901387-8901406 | None:intergenic | 15.0% |
| !!! | AAGTTTAGATTTTAAAACAA+AGG | - | chr4_2:8901449-8901468 | None:intergenic | 15.0% |
| !! | CTTATTATTCTTCTATTATT+AGG | + | chr4_2:8901475-8901494 | Msa0587410:intron | 15.0% |
| !!! | AAAAACTCTAAAATAAATGA+TGG | + | chr4_2:8901901-8901920 | Msa0587410:intron | 15.0% |
| !! | TATGAATGAAAATAACTATT+TGG | - | chr4_2:8901387-8901406 | None:intergenic | 15.0% |
| !!! | AAGTTTAGATTTTAAAACAA+AGG | - | chr4_2:8901449-8901468 | None:intergenic | 15.0% |
| !! | CTTATTATTCTTCTATTATT+AGG | + | chr4_2:8901475-8901494 | Msa0587410:intron | 15.0% |
| !!! | AAAAACTCTAAAATAAATGA+TGG | + | chr4_2:8901901-8901920 | Msa0587410:intron | 15.0% |
| !! | TAGTATATACAAACCAAAAT+AGG | - | chr4_2:8901543-8901562 | None:intergenic | 20.0% |
| !! | AAAAGTTTATTTATCTGCAT+AGG | - | chr4_2:8901653-8901672 | None:intergenic | 20.0% |
| !! | TAGTATATACAAACCAAAAT+AGG | - | chr4_2:8901543-8901562 | None:intergenic | 20.0% |
| !! | AAAAGTTTATTTATCTGCAT+AGG | - | chr4_2:8901653-8901672 | None:intergenic | 20.0% |
| !!! | AGCTTTTTAATTACCTGATT+TGG | - | chr4_2:8901292-8901311 | None:intergenic | 25.0% |
| !! | CATCATTCATTTCTTTTGTT+TGG | + | chr4_2:8901414-8901433 | Msa0587410:intron | 25.0% |
| !! | ATAATTGTCAACTCCTATTT+TGG | + | chr4_2:8901527-8901546 | Msa0587410:intron | 25.0% |
| ! | AAAAATAAGTCAATCCAAAC+AGG | + | chr4_2:8901846-8901865 | Msa0587410:intron | 25.0% |
| !!! | TGTTTTTAATGTGTGTGAAA+TGG | + | chr4_2:8901979-8901998 | Msa0587410:intron | 25.0% |
| ! | TAAATAAGTGCATGAATTGT+AGG | + | chr4_2:8902182-8902201 | Msa0587410:CDS | 25.0% |
| !!! | AGCTTTTTAATTACCTGATT+TGG | - | chr4_2:8901292-8901311 | None:intergenic | 25.0% |
| !! | CATCATTCATTTCTTTTGTT+TGG | + | chr4_2:8901414-8901433 | Msa0587410:intron | 25.0% |
| !! | ATAATTGTCAACTCCTATTT+TGG | + | chr4_2:8901527-8901546 | Msa0587410:intron | 25.0% |
| ! | AAAAATAAGTCAATCCAAAC+AGG | + | chr4_2:8901846-8901865 | Msa0587410:intron | 25.0% |
| !!! | TGTTTTTAATGTGTGTGAAA+TGG | + | chr4_2:8901979-8901998 | Msa0587410:intron | 25.0% |
| ! | TAAATAAGTGCATGAATTGT+AGG | + | chr4_2:8902182-8902201 | Msa0587410:CDS | 25.0% |
| !!! | CAGTTTTGTTTAGCTTGAAT+AGG | - | chr4_2:8901330-8901349 | None:intergenic | 30.0% |
| ATGCACTTATTTAAACAAGC+AGG | - | chr4_2:8902175-8902194 | None:intergenic | 30.0% | |
| ATACTTATTACCTTCTAGCA+TGG | + | chr4_2:8902278-8902297 | Msa0587410:CDS | 30.0% | |
| !!! | CAGTTTTGTTTAGCTTGAAT+AGG | - | chr4_2:8901330-8901349 | None:intergenic | 30.0% |
| ATGCACTTATTTAAACAAGC+AGG | - | chr4_2:8902175-8902194 | None:intergenic | 30.0% | |
| ATACTTATTACCTTCTAGCA+TGG | + | chr4_2:8902278-8902297 | Msa0587410:CDS | 30.0% | |
| TTTGAGTCCATGTAGATGAT+AGG | - | chr4_2:8901217-8901236 | None:intergenic | 35.0% | |
| !! | GTTTAGCTTGAATAGGTATG+AGG | - | chr4_2:8901323-8901342 | None:intergenic | 35.0% |
| AAGACAGGAAAATGGTGATA+GGG | + | chr4_2:8902140-8902159 | Msa0587410:CDS | 35.0% | |
| CACTTATTTAAACAAGCAGG+AGG | - | chr4_2:8902172-8902191 | None:intergenic | 35.0% | |
| ACAACCAAAGTTGCTATACA+AGG | - | chr4_2:8902208-8902227 | None:intergenic | 35.0% | |
| ! | AGTTACAAAGTGTTATCTCG+TGG | + | chr4_2:8902244-8902263 | Msa0587410:CDS | 35.0% |
| ! | GTTACAAAGTGTTATCTCGT+GGG | + | chr4_2:8902245-8902264 | Msa0587410:CDS | 35.0% |
| ! | TTACAAAGTGTTATCTCGTG+GGG | + | chr4_2:8902246-8902265 | Msa0587410:CDS | 35.0% |
| ATCTACATTTCCATGCTAGA+AGG | - | chr4_2:8902291-8902310 | None:intergenic | 35.0% | |
| TAGCATGGAAATGTAGATGT+GGG | + | chr4_2:8902293-8902312 | Msa0587410:CDS | 35.0% | |
| GAAATGTAGATGTGGGAATA+AGG | + | chr4_2:8902300-8902319 | Msa0587410:CDS | 35.0% | |
| TTTGAGTCCATGTAGATGAT+AGG | - | chr4_2:8901217-8901236 | None:intergenic | 35.0% | |
| !! | GTTTAGCTTGAATAGGTATG+AGG | - | chr4_2:8901323-8901342 | None:intergenic | 35.0% |
| AAGACAGGAAAATGGTGATA+GGG | + | chr4_2:8902140-8902159 | Msa0587410:CDS | 35.0% | |
| CACTTATTTAAACAAGCAGG+AGG | - | chr4_2:8902172-8902191 | None:intergenic | 35.0% | |
| ACAACCAAAGTTGCTATACA+AGG | - | chr4_2:8902208-8902227 | None:intergenic | 35.0% | |
| ! | AGTTACAAAGTGTTATCTCG+TGG | + | chr4_2:8902244-8902263 | Msa0587410:CDS | 35.0% |
| ! | GTTACAAAGTGTTATCTCGT+GGG | + | chr4_2:8902245-8902264 | Msa0587410:CDS | 35.0% |
| ! | TTACAAAGTGTTATCTCGTG+GGG | + | chr4_2:8902246-8902265 | Msa0587410:CDS | 35.0% |
| ATCTACATTTCCATGCTAGA+AGG | - | chr4_2:8902291-8902310 | None:intergenic | 35.0% | |
| TAGCATGGAAATGTAGATGT+GGG | + | chr4_2:8902293-8902312 | Msa0587410:CDS | 35.0% | |
| GAAATGTAGATGTGGGAATA+AGG | + | chr4_2:8902300-8902319 | Msa0587410:CDS | 35.0% | |
| TGTAGATGATAGGATCTAGC+AGG | - | chr4_2:8901207-8901226 | None:intergenic | 40.0% | |
| GCTAGATCCTATCATCTACA+TGG | + | chr4_2:8901207-8901226 | Msa0587410:CDS | 40.0% | |
| !! | TGCACGCTATTTTCCAAATC+AGG | + | chr4_2:8901276-8901295 | Msa0587410:CDS | 40.0% |
| TTGAATAGGTATGAGGTTGG+TGG | - | chr4_2:8901316-8901335 | None:intergenic | 40.0% | |
| AGCTTGAATAGGTATGAGGT+TGG | - | chr4_2:8901319-8901338 | None:intergenic | 40.0% | |
| TTATTGACCTAGGACCTGTT+TGG | - | chr4_2:8901863-8901882 | None:intergenic | 40.0% | |
| !! | CATGTGTCACTTATTGACCT+AGG | - | chr4_2:8901873-8901892 | None:intergenic | 40.0% |
| GAATGTGTATATGCTTGAGC+AGG | + | chr4_2:8902082-8902101 | Msa0587410:intron | 40.0% | |
| GTTGGAGAGAAATGATAGCT+TGG | + | chr4_2:8902117-8902136 | Msa0587410:CDS | 40.0% | |
| GAAATGATAGCTTGGAAGAC+AGG | + | chr4_2:8902125-8902144 | Msa0587410:CDS | 40.0% | |
| TAGCTTGGAAGACAGGAAAA+TGG | + | chr4_2:8902132-8902151 | Msa0587410:CDS | 40.0% | |
| GAAGACAGGAAAATGGTGAT+AGG | + | chr4_2:8902139-8902158 | Msa0587410:CDS | 40.0% | |
| ! | TAGGCCTTGTATAGCAACTT+TGG | + | chr4_2:8902201-8902220 | Msa0587410:CDS | 40.0% |
| CTAGCATGGAAATGTAGATG+TGG | + | chr4_2:8902292-8902311 | Msa0587410:CDS | 40.0% | |
| TGTAGATGATAGGATCTAGC+AGG | - | chr4_2:8901207-8901226 | None:intergenic | 40.0% | |
| GCTAGATCCTATCATCTACA+TGG | + | chr4_2:8901207-8901226 | Msa0587410:CDS | 40.0% | |
| !! | TGCACGCTATTTTCCAAATC+AGG | + | chr4_2:8901276-8901295 | Msa0587410:CDS | 40.0% |
| TTGAATAGGTATGAGGTTGG+TGG | - | chr4_2:8901316-8901335 | None:intergenic | 40.0% | |
| AGCTTGAATAGGTATGAGGT+TGG | - | chr4_2:8901319-8901338 | None:intergenic | 40.0% | |
| TTATTGACCTAGGACCTGTT+TGG | - | chr4_2:8901863-8901882 | None:intergenic | 40.0% | |
| !! | CATGTGTCACTTATTGACCT+AGG | - | chr4_2:8901873-8901892 | None:intergenic | 40.0% |
| GAATGTGTATATGCTTGAGC+AGG | + | chr4_2:8902082-8902101 | Msa0587410:intron | 40.0% | |
| GTTGGAGAGAAATGATAGCT+TGG | + | chr4_2:8902117-8902136 | Msa0587410:CDS | 40.0% | |
| GAAATGATAGCTTGGAAGAC+AGG | + | chr4_2:8902125-8902144 | Msa0587410:CDS | 40.0% | |
| TAGCTTGGAAGACAGGAAAA+TGG | + | chr4_2:8902132-8902151 | Msa0587410:CDS | 40.0% | |
| GAAGACAGGAAAATGGTGAT+AGG | + | chr4_2:8902139-8902158 | Msa0587410:CDS | 40.0% | |
| ! | TAGGCCTTGTATAGCAACTT+TGG | + | chr4_2:8902201-8902220 | Msa0587410:CDS | 40.0% |
| CTAGCATGGAAATGTAGATG+TGG | + | chr4_2:8902292-8902311 | Msa0587410:CDS | 40.0% | |
| AGATGATAGGATCTAGCAGG+TGG | - | chr4_2:8901204-8901223 | None:intergenic | 45.0% | |
| AGTCAATCCAAACAGGTCCT+AGG | + | chr4_2:8901853-8901872 | Msa0587410:intron | 45.0% | |
| TGTGTATATGCTTGAGCAGG+TGG | + | chr4_2:8902085-8902104 | Msa0587410:intron | 45.0% | |
| AGATGATAGGATCTAGCAGG+TGG | - | chr4_2:8901204-8901223 | None:intergenic | 45.0% | |
| AGTCAATCCAAACAGGTCCT+AGG | + | chr4_2:8901853-8901872 | Msa0587410:intron | 45.0% | |
| TGTGTATATGCTTGAGCAGG+TGG | + | chr4_2:8902085-8902104 | Msa0587410:intron | 45.0% | |
| TGCTTGAGCAGGTGGATCAT+TGG | + | chr4_2:8902093-8902112 | Msa0587410:intron | 50.0% | |
| AGCAGGTGGATCATTGGTGT+TGG | + | chr4_2:8902099-8902118 | Msa0587410:intron | 50.0% | |
| TGCTTGAGCAGGTGGATCAT+TGG | + | chr4_2:8902093-8902112 | Msa0587410:intron | 50.0% | |
| AGCAGGTGGATCATTGGTGT+TGG | + | chr4_2:8902099-8902118 | Msa0587410:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 8901195 | 8902336 | 8901195 | ID=Msa0587410;Name=Msa0587410 |
| chr4_2 | mRNA | 8901195 | 8902336 | 8901195 | ID=Msa0587410-mRNA-1;Parent=Msa0587410;Name=Msa0587410-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|111 |
| chr4_2 | exon | 8901195 | 8901297 | 8901195 | ID=Msa0587410-mRNA-1:exon:2461;Parent=Msa0587410-mRNA-1 |
| chr4_2 | exon | 8902104 | 8902336 | 8902104 | ID=Msa0587410-mRNA-1:exon:2462;Parent=Msa0587410-mRNA-1 |
| chr4_2 | CDS | 8901195 | 8901297 | 8901195 | ID=Msa0587410-mRNA-1:cds;Parent=Msa0587410-mRNA-1 |
| chr4_2 | CDS | 8902104 | 8902336 | 8902104 | ID=Msa0587410-mRNA-1:cds;Parent=Msa0587410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
