AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0667290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0667290 MtrunA17_Chr3g0128271 58.115 191 61 4 1 174 1 189 1.54e-65 206
Msa0667290 MtrunA17_Chr3g0128221 36.681 229 89 4 1 174 1 228 1.55e-35 124
Msa0667290 MtrunA17_Chr3g0128211 45.714 140 68 2 40 174 103 239 6.03e-32 115
Msa0667290 MtrunA17_Chr3g0128251 39.865 148 69 3 1 129 1 147 3.64e-24 93.6
Msa0667290 MtrunA17_Chr2g0312431 43.421 76 42 1 46 121 64 138 1.53e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0667290 AT4G27585.1 53.205 156 69 3 21 174 33 186 2.18e-49 164
Msa0667290 AT5G54100.1 54.478 134 59 2 41 174 98 229 8.34e-44 149

Find 65 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCAAACCTTCAACGTATA+TGG 0.196415 4_4:+7511977 None:intergenic
TTATTCTCCACAACTACTTT+TGG 0.242500 4_4:-7511756 Msa0667290:CDS
AACGCTCGATGCAAAGATTT+TGG 0.248278 4_4:-7511725 Msa0667290:CDS
CCTAATGATTATACCTTTCA+TGG 0.250289 4_4:-7511667 Msa0667290:three_prime_UTR
CTATGGTGTCGCCTGCTTAA+TGG 0.264755 4_4:+7511564 None:intergenic
CTAGTTGACATTAATATATC+TGG 0.286605 4_4:+7511512 None:intergenic
ACGCCGCCGCCGCCAAGGAT+TGG 0.329474 4_4:+7512343 None:intergenic
AAATTACTCCTCCGTTCAAT+TGG 0.344181 4_4:-7512187 Msa0667290:CDS
GTTGAATGACATCATCATAA+AGG 0.353425 4_4:+7511815 None:intergenic
CAACAAGAGGGATCAAATAA+TGG 0.362347 4_4:+7512084 None:intergenic
CGCCGCCGCCGCCAAGGATT+GGG 0.367198 4_4:+7512344 None:intergenic
ATTTATGATATGATATTGTT+TGG 0.371323 4_4:+7512442 None:intergenic
AGGGATCAAATAATGGATTC+CGG 0.377265 4_4:+7512091 None:intergenic
AAGTGCGATTCTCGCTGAAT+TGG 0.379009 4_4:+7512377 None:intergenic
CACCCAATCCTTGGCGGCGG+CGG 0.381758 4_4:-7512346 Msa0667290:CDS
AGCAAACCTTCAACGTATAT+GGG 0.387015 4_4:+7511978 None:intergenic
TCCTGTACTTGCTTCTTATG+AGG 0.398231 4_4:-7511848 Msa0667290:CDS
AATTACTCCTCCGTTCAATT+GGG 0.398483 4_4:-7512186 Msa0667290:CDS
CCATGAAAGGTATAATCATT+AGG 0.406342 4_4:+7511667 None:intergenic
TTATGATGATGTCATTCAAC+TGG 0.408703 4_4:-7511812 Msa0667290:CDS
TTCTTTACAGCTGTCGAGCC+AGG 0.427991 4_4:-7512308 Msa0667290:intron
GGGATCAAATAATGGATTCC+GGG 0.438866 4_4:+7512092 None:intergenic
TGAATTGGATTTCAAAATGT+TGG 0.446168 4_4:+7512392 None:intergenic
TGTCGCCTGCTTAATGGACT+CGG 0.450508 4_4:+7511570 None:intergenic
TTGAATGACATCATCATAAA+GGG 0.456161 4_4:+7511816 None:intergenic
AGAGATGCGCTTCATGAGGC+TGG 0.469216 4_4:+7512568 None:intergenic
TCCTTTCCCAAAAGTAGTTG+TGG 0.482236 4_4:+7511749 None:intergenic
TTCCATCACCCAATCCTTGG+CGG 0.493689 4_4:-7512352 Msa0667290:CDS
TGGTTCCATCACCCAATCCT+TGG 0.497489 4_4:-7512355 Msa0667290:CDS
TATGGGGACATTATCCTTAG+TGG 0.504839 4_4:+7511995 None:intergenic
TGAAGAATCAAAATAACAAA+AGG 0.517017 4_4:-7512511 Msa0667290:five_prime_UTR
TAACTAAATGCTACCATGAA+AGG 0.521492 4_4:+7511654 None:intergenic
AATGTCCCCATATACGTTGA+AGG 0.523280 4_4:-7511984 Msa0667290:CDS
GGAGGACGCCGCCGCCGCCA+AGG 0.524578 4_4:+7512338 None:intergenic
ATTACTCCTCCGTTCAATTG+GGG 0.531901 4_4:-7512185 Msa0667290:CDS
TGCTCACCTTGCTACCACTA+AGG 0.546637 4_4:-7512009 Msa0667290:CDS
ACCTCATAAGAAGCAAGTAC+AGG 0.549466 4_4:+7511847 None:intergenic
TTAGTGGTAGCAAGGTGAGC+AGG 0.552114 4_4:+7512011 None:intergenic
CCGACCCGAGTCCATTAAGC+AGG 0.562815 4_4:-7511575 Msa0667290:three_prime_UTR
TACTCCTCCGTTCAATTGGG+GGG 0.568985 4_4:-7512183 Msa0667290:CDS
GTCGCCTGCTTAATGGACTC+GGG 0.574205 4_4:+7511571 None:intergenic
CGCCGCCAAGGATTGGGTGA+TGG 0.582924 4_4:+7512350 None:intergenic
CATCACCCAATCCTTGGCGG+CGG 0.586028 4_4:-7512349 Msa0667290:CDS
GTGTACATTATTGAGCGATA+CGG 0.586615 4_4:-7512137 Msa0667290:CDS
CATTATCCTTAGTGGTAGCA+AGG 0.589535 4_4:+7512003 None:intergenic
AATGCGTAGTCAAGTCGGTA+AGG 0.593531 4_4:-7511779 Msa0667290:CDS
TTCACCACAGGAGGAATCCA+TGG 0.594068 4_4:+7512530 None:intergenic
TTACTCCTCCGTTCAATTGG+GGG 0.600597 4_4:-7512184 Msa0667290:CDS
ACCGACTTGACTACGCATTG+TGG 0.604609 4_4:+7511783 None:intergenic
TAAGCAATTCTATCAACAAG+AGG 0.607348 4_4:+7512071 None:intergenic
AAGCAAAATCTAGCTCCGCT+CGG 0.609823 4_4:-7511600 Msa0667290:three_prime_UTR
AGGAATCCATGGTACTGCAG+TGG 0.618978 4_4:+7512541 None:intergenic
CCTGCTTAATGGACTCGGGT+CGG 0.619339 4_4:+7511575 None:intergenic
CTGACTCCACTGCAGTACCA+TGG 0.623366 4_4:-7512547 Msa0667290:five_prime_UTR
AATTCAGCGAGAATCGCACT+TGG 0.625061 4_4:-7512375 Msa0667290:CDS
AGTCAGAGATGCGCTTCATG+AGG 0.629511 4_4:+7512564 None:intergenic
ACCACAATGCGTAGTCAAGT+CGG 0.632581 4_4:-7511784 Msa0667290:CDS
AGTACCATGGATTCCTCCTG+TGG 0.640120 4_4:-7512534 Msa0667290:five_prime_UTR
AAGCAATTCTATCAACAAGA+GGG 0.648716 4_4:+7512072 None:intergenic
GCAAACCTTCAACGTATATG+GGG 0.650332 4_4:+7511979 None:intergenic
ATCAAATAATGGATTCCGGG+TGG 0.660823 4_4:+7512095 None:intergenic
ACAGCTGTAAAGAAATCGAA+CGG 0.677083 4_4:+7512317 None:intergenic
TATGTCAAGACTCTTCCACC+CGG 0.699326 4_4:-7512110 Msa0667290:CDS
GGACTCGGGTCGGATCCGAG+CGG 0.755684 4_4:+7511585 None:intergenic
GCTGTAAAGAAATCGAACGG+AGG 0.769648 4_4:+7512320 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTGAATTTTTTTTTATTA+GGG - chr4_4:7511809-7511828 Msa0667290:CDS 10.0%
!!! GATTGAATTTTTTTTTATTA+GGG - chr4_4:7511809-7511828 Msa0667290:CDS 10.0%
!! ATTTATGATATGATATTGTT+TGG + chr4_4:7511586-7511605 None:intergenic 15.0%
!!! GGATTGAATTTTTTTTTATT+AGG - chr4_4:7511808-7511827 Msa0667290:CDS 15.0%
!! CACTTCAAAAAAATAAAAAA+AGG + chr4_4:7512089-7512108 None:intergenic 15.0%
!!! CTTTTTTTATTTTTTTGAAG+TGG - chr4_4:7512087-7512106 Msa0667290:CDS 15.0%
!! ATTTATGATATGATATTGTT+TGG + chr4_4:7511586-7511605 None:intergenic 15.0%
!!! GGATTGAATTTTTTTTTATT+AGG - chr4_4:7511808-7511827 Msa0667290:CDS 15.0%
!! CACTTCAAAAAAATAAAAAA+AGG + chr4_4:7512089-7512108 None:intergenic 15.0%
!!! CTTTTTTTATTTTTTTGAAG+TGG - chr4_4:7512087-7512106 Msa0667290:CDS 15.0%
!! TGAAGAATCAAAATAACAAA+AGG - chr4_4:7511514-7511533 Msa0667290:three_prime_UTR 20.0%
!!! ATTTTTTTGAAGTGGAAAAT+TGG - chr4_4:7512095-7512114 Msa0667290:CDS 20.0%
!! TGAAGAATCAAAATAACAAA+AGG - chr4_4:7511514-7511533 Msa0667290:three_prime_UTR 20.0%
!!! ATTTTTTTGAAGTGGAAAAT+TGG - chr4_4:7512095-7512114 Msa0667290:CDS 20.0%
! TGAATTGGATTTCAAAATGT+TGG + chr4_4:7511636-7511655 None:intergenic 25.0%
! CAATTTCATTCTCACTAATA+AGG - chr4_4:7511741-7511760 Msa0667290:CDS 25.0%
! TTGAATGACATCATCATAAA+GGG + chr4_4:7512212-7512231 None:intergenic 25.0%
!!! ACTTCAGTTTTAAGTTGTTT+CGG - chr4_4:7512393-7512412 Msa0667290:CDS 25.0%
!!! TTTTCGAGTAAAAAGAACTA+TGG + chr4_4:7512481-7512500 None:intergenic 25.0%
! CTAGTTGACATTAATATATC+TGG + chr4_4:7512516-7512535 None:intergenic 25.0%
! TGAATTGGATTTCAAAATGT+TGG + chr4_4:7511636-7511655 None:intergenic 25.0%
! CAATTTCATTCTCACTAATA+AGG - chr4_4:7511741-7511760 Msa0667290:CDS 25.0%
! TTGAATGACATCATCATAAA+GGG + chr4_4:7512212-7512231 None:intergenic 25.0%
!!! ACTTCAGTTTTAAGTTGTTT+CGG - chr4_4:7512393-7512412 Msa0667290:CDS 25.0%
!!! TTTTCGAGTAAAAAGAACTA+TGG + chr4_4:7512481-7512500 None:intergenic 25.0%
! CTAGTTGACATTAATATATC+TGG + chr4_4:7512516-7512535 None:intergenic 25.0%
!!! TTATTTTGATTCTTCACCAC+AGG + chr4_4:7511510-7511529 None:intergenic 30.0%
AAGCAATTCTATCAACAAGA+GGG + chr4_4:7511956-7511975 None:intergenic 30.0%
TAAGCAATTCTATCAACAAG+AGG + chr4_4:7511957-7511976 None:intergenic 30.0%
!!! TGAAGGTTTGCTTTTTTTTG+AGG - chr4_4:7512058-7512077 Msa0667290:CDS 30.0%
GTTGAATGACATCATCATAA+AGG + chr4_4:7512213-7512232 None:intergenic 30.0%
! TTATGATGATGTCATTCAAC+TGG - chr4_4:7512213-7512232 Msa0667290:intron 30.0%
! TTATTCTCCACAACTACTTT+TGG - chr4_4:7512269-7512288 Msa0667290:intron 30.0%
!! TATTCTCCACAACTACTTTT+GGG - chr4_4:7512270-7512289 Msa0667290:intron 30.0%
! CCATGAAAGGTATAATCATT+AGG + chr4_4:7512361-7512380 None:intergenic 30.0%
CCTAATGATTATACCTTTCA+TGG - chr4_4:7512358-7512377 Msa0667290:CDS 30.0%
TAACTAAATGCTACCATGAA+AGG + chr4_4:7512374-7512393 None:intergenic 30.0%
!!! TTATTTTGATTCTTCACCAC+AGG + chr4_4:7511510-7511529 None:intergenic 30.0%
AAGCAATTCTATCAACAAGA+GGG + chr4_4:7511956-7511975 None:intergenic 30.0%
TAAGCAATTCTATCAACAAG+AGG + chr4_4:7511957-7511976 None:intergenic 30.0%
!!! TGAAGGTTTGCTTTTTTTTG+AGG - chr4_4:7512058-7512077 Msa0667290:CDS 30.0%
GTTGAATGACATCATCATAA+AGG + chr4_4:7512213-7512232 None:intergenic 30.0%
! TTATGATGATGTCATTCAAC+TGG - chr4_4:7512213-7512232 Msa0667290:intron 30.0%
! TTATTCTCCACAACTACTTT+TGG - chr4_4:7512269-7512288 Msa0667290:intron 30.0%
!! TATTCTCCACAACTACTTTT+GGG - chr4_4:7512270-7512289 Msa0667290:intron 30.0%
! CCATGAAAGGTATAATCATT+AGG + chr4_4:7512361-7512380 None:intergenic 30.0%
CCTAATGATTATACCTTTCA+TGG - chr4_4:7512358-7512377 Msa0667290:CDS 30.0%
TAACTAAATGCTACCATGAA+AGG + chr4_4:7512374-7512393 None:intergenic 30.0%
ACAGCTGTAAAGAAATCGAA+CGG + chr4_4:7511711-7511730 None:intergenic 35.0%
TAGTGAGAATGAAATTGACC+TGG + chr4_4:7511738-7511757 None:intergenic 35.0%
AAATTACTCCTCCGTTCAAT+TGG - chr4_4:7511838-7511857 Msa0667290:CDS 35.0%
AATTACTCCTCCGTTCAATT+GGG - chr4_4:7511839-7511858 Msa0667290:CDS 35.0%
GTGTACATTATTGAGCGATA+CGG - chr4_4:7511888-7511907 Msa0667290:intron 35.0%
!! AGGGATCAAATAATGGATTC+CGG + chr4_4:7511937-7511956 None:intergenic 35.0%
CAACAAGAGGGATCAAATAA+TGG + chr4_4:7511944-7511963 None:intergenic 35.0%
AGCAAACCTTCAACGTATAT+GGG + chr4_4:7512050-7512069 None:intergenic 35.0%
AAGCAAACCTTCAACGTATA+TGG + chr4_4:7512051-7512070 None:intergenic 35.0%
!!! TGTGTTTTTAGTGCAGATTG+TGG - chr4_4:7512153-7512172 Msa0667290:CDS 35.0%
!!! AGTTTTAAGTTGTTTCGGAC+TGG - chr4_4:7512398-7512417 Msa0667290:CDS 35.0%
ACAGCTGTAAAGAAATCGAA+CGG + chr4_4:7511711-7511730 None:intergenic 35.0%
TAGTGAGAATGAAATTGACC+TGG + chr4_4:7511738-7511757 None:intergenic 35.0%
AAATTACTCCTCCGTTCAAT+TGG - chr4_4:7511838-7511857 Msa0667290:CDS 35.0%
AATTACTCCTCCGTTCAATT+GGG - chr4_4:7511839-7511858 Msa0667290:CDS 35.0%
GTGTACATTATTGAGCGATA+CGG - chr4_4:7511888-7511907 Msa0667290:intron 35.0%
!! AGGGATCAAATAATGGATTC+CGG + chr4_4:7511937-7511956 None:intergenic 35.0%
CAACAAGAGGGATCAAATAA+TGG + chr4_4:7511944-7511963 None:intergenic 35.0%
AGCAAACCTTCAACGTATAT+GGG + chr4_4:7512050-7512069 None:intergenic 35.0%
AAGCAAACCTTCAACGTATA+TGG + chr4_4:7512051-7512070 None:intergenic 35.0%
!!! TGTGTTTTTAGTGCAGATTG+TGG - chr4_4:7512153-7512172 Msa0667290:CDS 35.0%
!!! AGTTTTAAGTTGTTTCGGAC+TGG - chr4_4:7512398-7512417 Msa0667290:CDS 35.0%
!! TTTTGATTCTTCACCACAGG+AGG + chr4_4:7511507-7511526 None:intergenic 40.0%
! TCTCTTCCTTTTCGCGAAAA+TGG - chr4_4:7511610-7511629 Msa0667290:three_prime_UTR 40.0%
ATTACTCCTCCGTTCAATTG+GGG - chr4_4:7511840-7511859 Msa0667290:CDS 40.0%
!! ATCAAATAATGGATTCCGGG+TGG + chr4_4:7511933-7511952 None:intergenic 40.0%
!! GGGATCAAATAATGGATTCC+GGG + chr4_4:7511936-7511955 None:intergenic 40.0%
CATTATCCTTAGTGGTAGCA+AGG + chr4_4:7512025-7512044 None:intergenic 40.0%
TATGGGGACATTATCCTTAG+TGG + chr4_4:7512033-7512052 None:intergenic 40.0%
AATGTCCCCATATACGTTGA+AGG - chr4_4:7512041-7512060 Msa0667290:CDS 40.0%
GCAAACCTTCAACGTATATG+GGG + chr4_4:7512049-7512068 None:intergenic 40.0%
TCCTGTACTTGCTTCTTATG+AGG - chr4_4:7512177-7512196 Msa0667290:CDS 40.0%
ACCTCATAAGAAGCAAGTAC+AGG + chr4_4:7512181-7512200 None:intergenic 40.0%
!! TCCACAACTACTTTTGGGAA+AGG - chr4_4:7512275-7512294 Msa0667290:intron 40.0%
TCCTTTCCCAAAAGTAGTTG+TGG + chr4_4:7512279-7512298 None:intergenic 40.0%
! AACGCTCGATGCAAAGATTT+TGG - chr4_4:7512300-7512319 Msa0667290:intron 40.0%
!! TTTTGATTCTTCACCACAGG+AGG + chr4_4:7511507-7511526 None:intergenic 40.0%
! TCTCTTCCTTTTCGCGAAAA+TGG - chr4_4:7511610-7511629 Msa0667290:three_prime_UTR 40.0%
ATTACTCCTCCGTTCAATTG+GGG - chr4_4:7511840-7511859 Msa0667290:CDS 40.0%
!! ATCAAATAATGGATTCCGGG+TGG + chr4_4:7511933-7511952 None:intergenic 40.0%
!! GGGATCAAATAATGGATTCC+GGG + chr4_4:7511936-7511955 None:intergenic 40.0%
CATTATCCTTAGTGGTAGCA+AGG + chr4_4:7512025-7512044 None:intergenic 40.0%
TATGGGGACATTATCCTTAG+TGG + chr4_4:7512033-7512052 None:intergenic 40.0%
AATGTCCCCATATACGTTGA+AGG - chr4_4:7512041-7512060 Msa0667290:CDS 40.0%
GCAAACCTTCAACGTATATG+GGG + chr4_4:7512049-7512068 None:intergenic 40.0%
TCCTGTACTTGCTTCTTATG+AGG - chr4_4:7512177-7512196 Msa0667290:CDS 40.0%
ACCTCATAAGAAGCAAGTAC+AGG + chr4_4:7512181-7512200 None:intergenic 40.0%
!! TCCACAACTACTTTTGGGAA+AGG - chr4_4:7512275-7512294 Msa0667290:intron 40.0%
TCCTTTCCCAAAAGTAGTTG+TGG + chr4_4:7512279-7512298 None:intergenic 40.0%
! AACGCTCGATGCAAAGATTT+TGG - chr4_4:7512300-7512319 Msa0667290:intron 40.0%
!! TGTTGGCCATTTTCGCGAAA+AGG + chr4_4:7511619-7511638 None:intergenic 45.0%
AAGTGCGATTCTCGCTGAAT+TGG + chr4_4:7511651-7511670 None:intergenic 45.0%
AATTCAGCGAGAATCGCACT+TGG - chr4_4:7511650-7511669 Msa0667290:three_prime_UTR 45.0%
GCTGTAAAGAAATCGAACGG+AGG + chr4_4:7511708-7511727 None:intergenic 45.0%
TTACTCCTCCGTTCAATTGG+GGG - chr4_4:7511841-7511860 Msa0667290:CDS 45.0%
AACACAACCCCCCAATTGAA+CGG + chr4_4:7511852-7511871 None:intergenic 45.0%
TATGTCAAGACTCTTCCACC+CGG - chr4_4:7511915-7511934 Msa0667290:intron 45.0%
ACCACAATGCGTAGTCAAGT+CGG - chr4_4:7512241-7512260 Msa0667290:intron 45.0%
AATGCGTAGTCAAGTCGGTA+AGG - chr4_4:7512246-7512265 Msa0667290:intron 45.0%
! GCGGAGCTAGATTTTGCTTT+AGG + chr4_4:7512424-7512443 None:intergenic 45.0%
! AAGCAAAATCTAGCTCCGCT+CGG - chr4_4:7512425-7512444 Msa0667290:five_prime_UTR 45.0%
!! TGTTGGCCATTTTCGCGAAA+AGG + chr4_4:7511619-7511638 None:intergenic 45.0%
AAGTGCGATTCTCGCTGAAT+TGG + chr4_4:7511651-7511670 None:intergenic 45.0%
AATTCAGCGAGAATCGCACT+TGG - chr4_4:7511650-7511669 Msa0667290:three_prime_UTR 45.0%
GCTGTAAAGAAATCGAACGG+AGG + chr4_4:7511708-7511727 None:intergenic 45.0%
TTACTCCTCCGTTCAATTGG+GGG - chr4_4:7511841-7511860 Msa0667290:CDS 45.0%
AACACAACCCCCCAATTGAA+CGG + chr4_4:7511852-7511871 None:intergenic 45.0%
TATGTCAAGACTCTTCCACC+CGG - chr4_4:7511915-7511934 Msa0667290:intron 45.0%
ACCACAATGCGTAGTCAAGT+CGG - chr4_4:7512241-7512260 Msa0667290:intron 45.0%
AATGCGTAGTCAAGTCGGTA+AGG - chr4_4:7512246-7512265 Msa0667290:intron 45.0%
! GCGGAGCTAGATTTTGCTTT+AGG + chr4_4:7512424-7512443 None:intergenic 45.0%
! AAGCAAAATCTAGCTCCGCT+CGG - chr4_4:7512425-7512444 Msa0667290:five_prime_UTR 45.0%
!!! TTTGTTTTTATTAATTTAAT+TGG - chr4_4:7511787-7511806 Msa0667290:CDS 5.0%
!!! TTTGTTTTTATTAATTTAAT+TGG - chr4_4:7511787-7511806 Msa0667290:CDS 5.0%
AGTCAGAGATGCGCTTCATG+AGG + chr4_4:7511464-7511483 None:intergenic 50.0%
AGGAATCCATGGTACTGCAG+TGG + chr4_4:7511487-7511506 None:intergenic 50.0%
AGTACCATGGATTCCTCCTG+TGG - chr4_4:7511491-7511510 Msa0667290:three_prime_UTR 50.0%
TTCACCACAGGAGGAATCCA+TGG + chr4_4:7511498-7511517 None:intergenic 50.0%
TGGTTCCATCACCCAATCCT+TGG - chr4_4:7511670-7511689 Msa0667290:three_prime_UTR 50.0%
TTCCATCACCCAATCCTTGG+CGG - chr4_4:7511673-7511692 Msa0667290:three_prime_UTR 50.0%
TTCTTTACAGCTGTCGAGCC+AGG - chr4_4:7511717-7511736 Msa0667290:CDS 50.0%
TACTCCTCCGTTCAATTGGG+GGG - chr4_4:7511842-7511861 Msa0667290:CDS 50.0%
TTAGTGGTAGCAAGGTGAGC+AGG + chr4_4:7512017-7512036 None:intergenic 50.0%
TGCTCACCTTGCTACCACTA+AGG - chr4_4:7512016-7512035 Msa0667290:CDS 50.0%
!! ACCGACTTGACTACGCATTG+TGG + chr4_4:7512245-7512264 None:intergenic 50.0%
!! TGTCGCCTGCTTAATGGACT+CGG + chr4_4:7512458-7512477 None:intergenic 50.0%
!! CTATGGTGTCGCCTGCTTAA+TGG + chr4_4:7512464-7512483 None:intergenic 50.0%
AGTCAGAGATGCGCTTCATG+AGG + chr4_4:7511464-7511483 None:intergenic 50.0%
AGGAATCCATGGTACTGCAG+TGG + chr4_4:7511487-7511506 None:intergenic 50.0%
AGTACCATGGATTCCTCCTG+TGG - chr4_4:7511491-7511510 Msa0667290:three_prime_UTR 50.0%
TTCACCACAGGAGGAATCCA+TGG + chr4_4:7511498-7511517 None:intergenic 50.0%
TGGTTCCATCACCCAATCCT+TGG - chr4_4:7511670-7511689 Msa0667290:three_prime_UTR 50.0%
TTCCATCACCCAATCCTTGG+CGG - chr4_4:7511673-7511692 Msa0667290:three_prime_UTR 50.0%
TTCTTTACAGCTGTCGAGCC+AGG - chr4_4:7511717-7511736 Msa0667290:CDS 50.0%
TACTCCTCCGTTCAATTGGG+GGG - chr4_4:7511842-7511861 Msa0667290:CDS 50.0%
TTAGTGGTAGCAAGGTGAGC+AGG + chr4_4:7512017-7512036 None:intergenic 50.0%
TGCTCACCTTGCTACCACTA+AGG - chr4_4:7512016-7512035 Msa0667290:CDS 50.0%
!! ACCGACTTGACTACGCATTG+TGG + chr4_4:7512245-7512264 None:intergenic 50.0%
!! TGTCGCCTGCTTAATGGACT+CGG + chr4_4:7512458-7512477 None:intergenic 50.0%
!! CTATGGTGTCGCCTGCTTAA+TGG + chr4_4:7512464-7512483 None:intergenic 50.0%
AGAGATGCGCTTCATGAGGC+TGG + chr4_4:7511460-7511479 None:intergenic 55.0%
CTGACTCCACTGCAGTACCA+TGG - chr4_4:7511478-7511497 Msa0667290:three_prime_UTR 55.0%
ACAACCCCCCAATTGAACGG+AGG + chr4_4:7511849-7511868 None:intergenic 55.0%
!! CCTGCTTAATGGACTCGGGT+CGG + chr4_4:7512453-7512472 None:intergenic 55.0%
!! GTCGCCTGCTTAATGGACTC+GGG + chr4_4:7512457-7512476 None:intergenic 55.0%
AGAGATGCGCTTCATGAGGC+TGG + chr4_4:7511460-7511479 None:intergenic 55.0%
CTGACTCCACTGCAGTACCA+TGG - chr4_4:7511478-7511497 Msa0667290:three_prime_UTR 55.0%
ACAACCCCCCAATTGAACGG+AGG + chr4_4:7511849-7511868 None:intergenic 55.0%
!! CCTGCTTAATGGACTCGGGT+CGG + chr4_4:7512453-7512472 None:intergenic 55.0%
!! GTCGCCTGCTTAATGGACTC+GGG + chr4_4:7512457-7512476 None:intergenic 55.0%
CATCACCCAATCCTTGGCGG+CGG - chr4_4:7511676-7511695 Msa0667290:three_prime_UTR 60.0%
CCGACCCGAGTCCATTAAGC+AGG - chr4_4:7512450-7512469 Msa0667290:five_prime_UTR 60.0%
CATCACCCAATCCTTGGCGG+CGG - chr4_4:7511676-7511695 Msa0667290:three_prime_UTR 60.0%
CCGACCCGAGTCCATTAAGC+AGG - chr4_4:7512450-7512469 Msa0667290:five_prime_UTR 60.0%
CGCCGCCAAGGATTGGGTGA+TGG + chr4_4:7511678-7511697 None:intergenic 65.0%
CGCCGCCAAGGATTGGGTGA+TGG + chr4_4:7511678-7511697 None:intergenic 65.0%
CACCCAATCCTTGGCGGCGG+CGG - chr4_4:7511679-7511698 Msa0667290:three_prime_UTR 70.0%
GGACTCGGGTCGGATCCGAG+CGG + chr4_4:7512443-7512462 None:intergenic 70.0%
CACCCAATCCTTGGCGGCGG+CGG - chr4_4:7511679-7511698 Msa0667290:three_prime_UTR 70.0%
GGACTCGGGTCGGATCCGAG+CGG + chr4_4:7512443-7512462 None:intergenic 70.0%
CGCCGCCGCCGCCAAGGATT+GGG + chr4_4:7511684-7511703 None:intergenic 75.0%
ACGCCGCCGCCGCCAAGGAT+TGG + chr4_4:7511685-7511704 None:intergenic 75.0%
CGCCGCCGCCGCCAAGGATT+GGG + chr4_4:7511684-7511703 None:intergenic 75.0%
ACGCCGCCGCCGCCAAGGAT+TGG + chr4_4:7511685-7511704 None:intergenic 75.0%
! GGAGGACGCCGCCGCCGCCA+AGG + chr4_4:7511690-7511709 None:intergenic 85.0%
! GGAGGACGCCGCCGCCGCCA+AGG + chr4_4:7511690-7511709 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 7511443 7512604 7511443 ID=Msa0667290;Name=Msa0667290
chr4_4 mRNA 7511443 7512604 7511443 ID=Msa0667290-mRNA-1;Parent=Msa0667290;Name=Msa0667290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=186|1|1|1|1|1|3|268|175
chr4_4 exon 7511443 7511878 7511443 ID=Msa0667290-mRNA-1:exon:2498;Parent=Msa0667290-mRNA-1
chr4_4 exon 7511968 7512217 7511968 ID=Msa0667290-mRNA-1:exon:2497;Parent=Msa0667290-mRNA-1
chr4_4 exon 7512309 7512604 7512309 ID=Msa0667290-mRNA-1:exon:2496;Parent=Msa0667290-mRNA-1
chr4_4 five_prime_UTR 7512419 7512604 7512419 ID=Msa0667290-mRNA-1:five_prime_utr;Parent=Msa0667290-mRNA-1
chr4_4 CDS 7512309 7512418 7512309 ID=Msa0667290-mRNA-1:cds;Parent=Msa0667290-mRNA-1
chr4_4 CDS 7511968 7512217 7511968 ID=Msa0667290-mRNA-1:cds;Parent=Msa0667290-mRNA-1
chr4_4 CDS 7511711 7511878 7511711 ID=Msa0667290-mRNA-1:cds;Parent=Msa0667290-mRNA-1
chr4_4 three_prime_UTR 7511443 7511710 7511443 ID=Msa0667290-mRNA-1:three_prime_utr;Parent=Msa0667290-mRNA-1
Gene Sequence

>Msa0667290

ATGGCCAACATTTTGAAATCCAATTCAGCGAGAATCGCACTTGGTTCCATCACCCAATCCTTGGCGGCGGCGGCGTCCTCCGTTCGATTTCTTTACAGCTGTCGAGCCAGGGATCGTGAAATTACTCCTCCGTTCAATTGGGGGGTTGTGTTTGTAGATTTTTCGAAAGTGTACATTATTGAGCGATACGGAAAGTATGTCAAGACTCTTCCACCCGGAATCCATTATTTGATCCCTCTTGTTGATAGAATTGCTTACGTTCATTCGATAATAAAAAATCAAAGTATCGAGATTCCTGCTCACCTTGCTACCACTAAGGATAATGTCCCCATATACGTTGAAGGTTTGCTTTTTTTTGAGATTGTGGATCCTGTACTTGCTTCTTATGAGGTTGAGACTACCCTTTATGATGATGTCATTCAACTGGCTCAAACCACAATGCGTAGTCAAGTCGGTAAGGTTATTCTCCACAACTACTTTTGGGAAAGGAGAACGCTCGATGCAAAGATTTTGGTAAGTTTAGTTTAA
Protein sequence

>Msa0667290

MANILKSNSARIALGSITQSLAAAASSVRFLYSCRARDREITPPFNWGVVFVDFSKVYIIERYGKYVKTLPPGIHYLIPLVDRIAYVHSIIKNQSIEIPAHLATTKDNVPIYVEGLLFFEIVDPVLASYEVETTLYDDVIQLAQTTMRSQVGKVILHNYFWERRTLDAKILVSLV*