AlfalfaGEDB | Msa0670320

M. sativa cultivar zhongmu-4 / Msa0670320

KEGG: -
GO: -
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670320	KEH29033.1	69.985	653	133	7	791	1396	890	1526	0.0	845
Msa0670320	KEH29033.1	75.626	759	134	4	1	726	805	1545	0.0	1109

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670320	sp\|V9M398\|RUN1_VITRO	31.949	313	169	5	77	354	538	841	1.62e-33	145
Msa0670320	sp\|V9M398\|RUN1_VITRO	28.528	326	175	6	810	1100	539	841	5.99e-25	117

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670320	A0A072UIW1	69.985	653	133	7	791	1396	890	1526	0.0	845
Msa0670320	A0A072UIW1	75.626	759	134	4	1	726	805	1545	0.0	1109

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0266670	Msa0670320	0.814549	1.455653e-51	-8.615850e-47
Msa0670320	Msa1170460	0.818995	1.465285e-52	-8.615850e-47
Msa0670320	Msa1448560	0.828128	1.067600e-54	-8.615850e-47
Msa0670320	Msa1463900	0.801245	9.861259e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670320	MtrunA17_Chr4g0009041	78.234	657	138	2	74	726	1027	1682	0.0	983
Msa0670320	MtrunA17_Chr4g0009041	67.951	649	130	5	810	1396	1031	1663	0.0	784
Msa0670320	MtrunA17_Chr4g0009041	85.714	77	11	0	1	77	850	926	1.05e-33	142
Msa0670320	MtrunA17_Chr4g0009041	82.222	45	8	0	768	812	658	702	1.95e-13	75.9
Msa0670320	MtrunA17_Chr4g0009041	47.500	80	39	2	1	77	346	425	2.93e-11	68.6
Msa0670320	MtrunA17_Chr4g0009361	76.935	659	127	3	75	726	32	672	0.0	978
Msa0670320	MtrunA17_Chr4g0009361	69.985	653	133	7	791	1396	17	653	0.0	845
Msa0670320	MtrunA17_Chr4g0009171	75.988	658	148	5	72	723	1244	1897	0.0	952
Msa0670320	MtrunA17_Chr4g0009171	64.209	651	150	8	810	1396	1250	1881	0.0	745
Msa0670320	MtrunA17_Chr4g0009171	83.258	221	35	2	77	297	522	740	4.35e-107	375

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0670320	AT5G36930.3	41.414	297	155	5	72	360	517	802	5.78e-50	194
Msa0670320	AT5G36930.3	40.532	301	153	6	790	1080	504	788	1.72e-47	186
Msa0670320	AT5G36930.3	48.052	77	40	0	1	77	344	420	1.06e-15	83.6
Msa0670320	AT5G36930.4	41.414	297	155	5	72	360	517	802	5.96e-50	194
Msa0670320	AT5G36930.4	40.532	301	153	6	790	1080	504	788	1.64e-47	187
Msa0670320	AT5G36930.4	48.052	77	40	0	1	77	344	420	9.40e-16	83.6
Msa0670320	AT5G36930.1	41.414	297	155	5	72	360	517	802	5.96e-50	194
Msa0670320	AT5G36930.1	40.532	301	153	6	790	1080	504	788	1.64e-47	187
Msa0670320	AT5G36930.1	48.052	77	40	0	1	77	344	420	9.40e-16	83.6
Msa0670320	AT5G36930.2	41.414	297	155	5	72	360	520	805	6.06e-50	194

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Find 302 sgRNAs with CRISPR-Local

Find 611 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTCGAATTCTTTGTG+TGG	0.572096	4_4:+12768362	Msa0670320:CDS
AAACTGTCCAAGTTTGTCTA+CGG	0.582572	4_4:+12762492	Msa0670320:CDS
AAATCTCTCTTAATTCAAAT+GGG	0.444215	4_4:+12763120	Msa0670320:CDS
AAATTTCTCTTAATTAAAAT+GGG	0.398389	4_4:+12768573	Msa0670320:CDS
AACACAAGCTGTCAAGGGAC+TGG	0.558389	4_4:+12761283	Msa0670320:CDS
AACACCTGAAACCATTGGGC+TGG	0.539914	4_4:+12764199	Msa0670320:CDS
AACACTGTCTACAGCAATAT+TGG	0.338391	4_4:-12768613	None:intergenic
AACACTGTCTACAGCAATAT+TGG	0.347449	4_4:-12763160	None:intergenic
AACAGAAGCTGTCAAGGGAC+TGG	0.529860	4_4:+12767304	Msa0670320:CDS
AACAGAGTGGCATAAAGTTT+TGG	0.304010	4_4:+12760704	Msa0670320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTCATGTACTTGATCAT+CGG	-	chr4_4:12760748-12760767	None:intergenic	30.0%
CTTTATTGGAATGGACAAAA+AGG	+	chr4_4:12760836-12760855	Msa0670320:intron	30.0%
ATACAAATATTGAATGGGTG+TGG	+	chr4_4:12760864-12760883	Msa0670320:intron	30.0%
TACAAATATTGAATGGGTGT+GGG	+	chr4_4:12760865-12760884	Msa0670320:intron	30.0%
TATTGTTCCTCTACACAAAA+TGG	-	chr4_4:12761791-12761810	None:intergenic	30.0%
TAGAGTAAACACAAGAAAGA+AGG	+	chr4_4:12761809-12761828	Msa0670320:intron	30.0%
ATCACTAACAACAGATAAGT+TGG	+	chr4_4:12762093-12762112	Msa0670320:intron	30.0%
TCACTAACAACAGATAAGTT+GGG	+	chr4_4:12762094-12762113	Msa0670320:intron	30.0%
TAGATTGGAAATTGGAAGAA+TGG	-	chr4_4:12762309-12762328	None:intergenic	30.0%
GAATAATAGTAAGAGAAGCT+TGG	-	chr4_4:12762347-12762366	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	12760543	12769784	12760543	ID=Msa0670320;Name=Msa0670320
chr4_4	mRNA	12760543	12769784	12760543	ID=Msa0670320-mRNA-1;Parent=Msa0670320;Name=Msa0670320-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0\|0.33\|0.3\|1\|1\|1\|10\|0\|1397
chr4_4	exon	12760543	12760773	12760543	ID=Msa0670320-mRNA-1:exon:3827;Parent=Msa0670320-mRNA-1
chr4_4	exon	12761281	12761550	12761281	ID=Msa0670320-mRNA-1:exon:3828;Parent=Msa0670320-mRNA-1
chr4_4	exon	12762391	12763188	12762391	ID=Msa0670320-mRNA-1:exon:3829;Parent=Msa0670320-mRNA-1
chr4_4	exon	12763504	12764393	12763504	ID=Msa0670320-mRNA-1:exon:3830;Parent=Msa0670320-mRNA-1
chr4_4	exon	12765961	12766198	12765961	ID=Msa0670320-mRNA-1:exon:3831;Parent=Msa0670320-mRNA-1
chr4_4	exon	12767302	12767613	12767302	ID=Msa0670320-mRNA-1:exon:3832;Parent=Msa0670320-mRNA-1
chr4_4	exon	12767844	12768641	12767844	ID=Msa0670320-mRNA-1:exon:3833;Parent=Msa0670320-mRNA-1
chr4_4	exon	12768944	12769019	12768944	ID=Msa0670320-mRNA-1:exon:3834;Parent=Msa0670320-mRNA-1
chr4_4	exon	12769122	12769244	12769122	ID=Msa0670320-mRNA-1:exon:3835;Parent=Msa0670320-mRNA-1
chr4_4	exon	12769327	12769784	12769327	ID=Msa0670320-mRNA-1:exon:3836;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12760543	12760773	12760543	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12761281	12761550	12761281	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12762391	12763188	12762391	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12763504	12764393	12763504	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12765961	12766198	12765961	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12767302	12767613	12767302	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12767844	12768641	12767844	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12768944	12769019	12768944	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12769122	12769244	12769122	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1
chr4_4	CDS	12769327	12769784	12769327	ID=Msa0670320-mRNA-1:cds;Parent=Msa0670320-mRNA-1

Gene Sequence

>Msa0670320

ATGGATGAAAGTGAATCTCTCAAGCTTTTCAGTTGGCATGCATTCAAGCAACCAAGTCCTATAGAAGATTTTGCTAAACATTCAACAGATGTGATTGCTTATTCTGGGGGATTGCCTCTAGCACTTGAAGTCATTGGGTCATATTTGTCTGGCTGTGAAATAACAGAGTGGCATAAAGTTTTGGAGAAACTCAAATGTATTCCCGATGATCAAGTACATGAGAAACTAAAAGGAACACAAGCTGTCAAGGGACTGGCTTTGGAGTTTCCGACAAGAGATTGTTTGGAAACAAAAGCATTTAAGAAAATGAATAAACTCAGATTACTTCGCCTTGCTGGGGTGAAGCTTAAAGGAGATTTTAAATATCTTTCAGGAGATCTGAAGTGGCTGTACTGGCATGGATTTCCAGAAACCTACATTCCTGCAGAATTTCAGCTAGGAAGTCTAGTTGTTATGGAATTAAAATATAGTAAGCTTATACAAATATGGAATAAGAGCCAGATGCTAGAAAATCTAAAAATTCTTAATCTTAGTCATTCTCTGGATTTGACTGAAACCCCAGACTTTTCATACTTACCTAATCTTGAAAGGCTCGTGCTCAAAAACTGTCCAAGTTTGTCTACGGTCTCCCATAGCATTGGATCTCTACATAAAATTCTTCTGATAAATTTGAGAGACTGCACAGGTCTTCGGAAACTTCCAAGAAACATCTACAAGTTAAAATCTCTAGAAACTTTGATTCTTTCAGGATGTTCCATGATTGACAAATTGGAGGAGGATGTGGAACAGATGGAATCATTGACAACCTTGATTGCGGATAAGACTGATATAACAAAAGTGCCATTTTCAATAGTAAGATTGAAAAGCATTGGATATATTTCTTTAAGTGGCTTTGAAGGATTTTCACGTGATGTATTCCCTTCTCTTATTCGGTCTTGGATGTCGCCAACAAATAATGTGATATCTCTAGTTCAGACATCTGTGTCCATGTCATCACTTGCTTCTTCCAGGGACCTTCAAAATCTTCGAATTCTTTTTGTGGAGTATGGTTCAGATCTACAACTAACTCAAGATATAGCAATATTTTTGGATGTATTAAAAGCCACAAGTGTTCAGAAATTAGAATCTGGTGCTAGTTCAATTACATCAGAGATCTCTGATATGTATGCTTCTCCGTTAATTGATGATTTCCGTGGTCAAGTTCTCACTTCAGGGTCAAAGAATCACTTGAAATCTCTCTTAATTCAAATGGGAACAAAATGTCATGTCTCCAATATTGCTGTAGACAGTGTTATACAGACTGCAGATGCATTTTGGGATTCGTTTGTGCTCCCCTGTGACAATAATCCTGAGTGGCTAACCTTCCATTGCAAAGGTTGTTCCATAATATTTGATCTTCCAACAATCAAGGGGCGAAACCTGAAGAGTATGATATTGTTAGTTGCTTATTACTCTTCCTCAGGAAGCATAACATCAGAAGGTTGCCAAGGTGTGTTGATCATAAATCATACAAAGACAACCATTCAGGCCTATAAAAAAGATACACTAACTTCTTTTGAGGATGAGGATTGGAAGAGCATAACATCAAATCTAGAACCTGGTAACAAAGTGGAGGTTATGGTTGTTTTTGGGGAGGGATTCATTGTAGAGAAGACAACAGTATCTCTCTTATATGATCAGCCAACCAACAATGAAATGGAATGTTGTCATGCAGTAGACCAGAAGGATGTCGTAGTTTCTGGAAAAGACGGTAATCATGTTTGTGTTTCTGGTGGTGATTGTGAAATCAGCAATCAGTTTAGAGAGGGACCGGCGAACCATTTGCAGATCACTGGGCAGTATGCCGATGTCGTGGGACTGGTACAGCTAGTACCGCATGACCTTGACCAGCCTGCGGAGGAAGTAGCTGAGGAGATTTGTGCAGCTGAAGTAGAGAATGGTCAGAAGCAACAGGTTCCTCTACCTGAGGGTCAGTCTGAGCTGCATGCAGCGGCAACACCTGAAACCATTGGGCTGGTTCTAGGAGCTCAGATTTTGGATGCCCTCAAAGAGCTCGGGGCTGATTTCGTCCGACTAGAGCAGACTGTTACAGCTAGGTTAAATGCAGTAGAGGACAAATTAGAGGAGCTCGAGCATGTCATTACCCAGATACCGCGTGGGTCGAGTTCCAAGTCCACTTCCATCCTTAGAAAAGCTTCAGAAAATGACAACATATACTGGCATATCATTCTTCCTATTATTTTGATAGATGCATATTGGAGAATGCTTACACATACAATTGAATGTGAGCCTTATCATTGGTTAATATTTTATAGCAACGAAAGTGACGATGTCAAGAATATTGTTGAACATGTCACCCGTTTGTTAGATAGGACAGAATTATTCGTTGCTGAACATCCAGTGGGTGTAGAATCTCGCGTGGAAGCTGGAACAGAAGCTGTCAAGGGACTGGCTTTGGAGTTTCCAAGAAAAGATTGTCTGGAAACTAAAGCATTTAAGAAGATGAACAAACTCAGATTGCTTCGCCTCGCTGGGGTGAAGCTTAAGGGAGATTTTAAATACCTTTCAGGAGATCTTAGGTGGCTGTACTGGCATGGATTTCCAAAAATCTACGCTCCCGCAGAATTTCAGCGAGAAAGTCTAGTGGCCGTTGAATTAAAATATAGTAAGCTAGCTCAAACGATTATGGAACAAGAGCCAGGTAAAATTAGTGATTTTAGTGGTATGAAGCACTACTTTATGCTAGAGAATCTAAAAATTCTTAATCTTAGTCATTCTCATAGTTTGACTGAAACCCTAGACTTTTCATACTTGCCAAATCTTGAAAAGCTAGTACTCAAAGACTGTCCAAGTTTGTCTACGGTCTCCCATAGCATTGGAACTCTGCATAAAATTCTTCTCATAAATTTGAGAGACTGCATAGGTCTTCGGAAGCTTCCAAGAAGCATCTATATATTAAAATCTCTAGAAACTTTGATTCTTTCAGGATGTTCCATGATTGACAAATTGGAGGATGACTTGGAACAAATGGACTCTTTGATTACGTTGATTGCAGATAAGACTGCTATAACAAAAGTGCCATTTTCAATAGTAAGATCGAAAAGCATTGGATGCATTTCTTTAAATGGCTTTGAAGGATTTACACGTGATGTATTCCCTTCTCTCATTCGCTCTTGGATGTCGCCATCTAATAATGTGATATCTCTTGTTCAAACATCGATGTCCGTGTCATCACTTGGTAATTATAAGGACCTTGAAAAACTTCGAATTCTTTGTGTGGAGTATGGTTCAGATCTACAACTAACTCAAGATACAGCAATATTTTTGGATGTATTAAAAGCCACAAGTTATCAGAAATTGGAAGCAAGTGCAAGTTCAACTAGATCAGAGACCTCTGATTTGCATGCTTCTCCCTCAATTGATGATTTCCGTGGTCAAGTTCGCACTTCAAGGTCAAAGAATCACTTGAAATTTCTCTTAATTAAAATGGGAACAAAATGCCATGTCTCCAATATTGCTGTAGACAGTGTTTTACAGACTGCAGATGCATCTTGGGATTCGCTTGTGCTCCCCTGCGACAATAATTCTGATTGGCTAACCTTCCATTGCGAAGAAGGTTGCCAAGGTGTGTTAATCATAAATTACACAAAGACAACCATTCAAGCATATAAAAGAGATACACTAACCTCATTTGAGGATGAGGATTGGCAGAGCATAACATCTAATCTAGAACCTGTCAACAAAGAAATGGAATGTTGCCATGTAGTAGACGAGGAGGATGTCGTTGTTTTTGGCAATGACGATAATCATGTTGGTGTTTCTGGTGGTGACAATAAAGTCATCAATCAGTTTAGAGAGGGAACAGTGAACCATTTGCAGATCACTTGGCCCACAGATGGGTGTTATGCTGCTGATGTCGTGGGACTGGTAGAGCTAGTACCGCGTGACCTTGACCAGCCTGCGGAGGCAGTAGCTGAGGAGATTTGTGCAGCTGAAGTAGAGAATGGTCAGAAGCAACAGGGTCCTCTACCCGAGGGTCAGTCTGAGCTGCAAGCAGCAGCCACACCTGAGACCATTGGACTGGTTCTAGGAGCTCAGATTTTGAATGCCCTCAAAGACCTCAGGACTGATTTCGTTCAACTAGAGCAGACTGTTACTGCTAGGTTAAACGCAGTAGAGGTCAAATTAGGGTAA

Protein sequence

>Msa0670320

MDESESLKLFSWHAFKQPSPIEDFAKHSTDVIAYSGGLPLALEVIGSYLSGCEITEWHKVLEKLKCIPDDQVHEKLKGTQAVKGLALEFPTRDCLETKAFKKMNKLRLLRLAGVKLKGDFKYLSGDLKWLYWHGFPETYIPAEFQLGSLVVMELKYSKLIQIWNKSQMLENLKILNLSHSLDLTETPDFSYLPNLERLVLKNCPSLSTVSHSIGSLHKILLINLRDCTGLRKLPRNIYKLKSLETLILSGCSMIDKLEEDVEQMESLTTLIADKTDITKVPFSIVRLKSIGYISLSGFEGFSRDVFPSLIRSWMSPTNNVISLVQTSVSMSSLASSRDLQNLRILFVEYGSDLQLTQDIAIFLDVLKATSVQKLESGASSITSEISDMYASPLIDDFRGQVLTSGSKNHLKSLLIQMGTKCHVSNIAVDSVIQTADAFWDSFVLPCDNNPEWLTFHCKGCSIIFDLPTIKGRNLKSMILLVAYYSSSGSITSEGCQGVLIINHTKTTIQAYKKDTLTSFEDEDWKSITSNLEPGNKVEVMVVFGEGFIVEKTTVSLLYDQPTNNEMECCHAVDQKDVVVSGKDGNHVCVSGGDCEISNQFREGPANHLQITGQYADVVGLVQLVPHDLDQPAEEVAEEICAAEVENGQKQQVPLPEGQSELHAAATPETIGLVLGAQILDALKELGADFVRLEQTVTARLNAVEDKLEELEHVITQIPRGSSSKSTSILRKASENDNIYWHIILPIILIDAYWRMLTHTIECEPYHWLIFYSNESDDVKNIVEHVTRLLDRTELFVAEHPVGVESRVEAGTEAVKGLALEFPRKDCLETKAFKKMNKLRLLRLAGVKLKGDFKYLSGDLRWLYWHGFPKIYAPAEFQRESLVAVELKYSKLAQTIMEQEPGKISDFSGMKHYFMLENLKILNLSHSHSLTETLDFSYLPNLEKLVLKDCPSLSTVSHSIGTLHKILLINLRDCIGLRKLPRSIYILKSLETLILSGCSMIDKLEDDLEQMDSLITLIADKTAITKVPFSIVRSKSIGCISLNGFEGFTRDVFPSLIRSWMSPSNNVISLVQTSMSVSSLGNYKDLEKLRILCVEYGSDLQLTQDTAIFLDVLKATSYQKLEASASSTRSETSDLHASPSIDDFRGQVRTSRSKNHLKFLLIKMGTKCHVSNIAVDSVLQTADASWDSLVLPCDNNSDWLTFHCEEGCQGVLIINYTKTTIQAYKRDTLTSFEDEDWQSITSNLEPVNKEMECCHVVDEEDVVVFGNDDNHVGVSGGDNKVINQFREGTVNHLQITWPTDGCYAADVVGLVELVPRDLDQPAEAVAEEICAAEVENGQKQQGPLPEGQSELQAAATPETIGLVLGAQILNALKDLRTDFVQLEQTVTARLNAVEVKLG*