AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0670670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0670670 MtrunA17_Chr4g0009811 94.667 75 4 0 1 75 1 75 2.75e-46 142
Msa0670670 MtrunA17_Chr4g0009821 90.667 75 7 0 1 75 1 75 4.97e-43 134
Msa0670670 MtrunA17_Chr7g0238571 50.725 69 31 2 8 75 12 78 5.49e-11 52.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCACAGAAACCGTGATTC+TGG 0.293571 4_4:+13262656 Msa0670670:CDS
CCATTTCAGCACCCTCAAAA+TGG 0.321140 4_4:-13262698 None:intergenic
TGCTATGGTTGCTTTGTGTT+TGG 0.374144 4_4:+13262968 Msa0670670:three_prime_UTR
CGTGATTCTGGGCGTTGGTC+CGG 0.396505 4_4:+13262668 Msa0670670:CDS
CCCTCAAAATGGATCTTTGC+CGG 0.397774 4_4:-13262687 None:intergenic
AGCAAAGAATAATATTTGAT+TGG 0.399667 4_4:-13261188 None:intergenic
AACAGCAGGTCTAGTGGGTT+GGG 0.444520 4_4:+13262618 Msa0670670:CDS
CACTTCACTTACAGTTGCAT+GGG 0.476971 4_4:-13262773 None:intergenic
CAACAGCAGGTCTAGTGGGT+TGG 0.483236 4_4:+13262617 Msa0670670:CDS
TTAATTAGAATAATATAAAG+TGG 0.488865 4_4:+13262894 Msa0670670:three_prime_UTR
ACCACAGAAACCGTGATTCT+GGG 0.490783 4_4:+13262657 Msa0670670:CDS
GCACTTCACTTACAGTTGCA+TGG 0.509517 4_4:-13262774 None:intergenic
TGCCTTGCAGATGCAACAGC+AGG 0.516088 4_4:+13262604 Msa0670670:intron
AGAATAATATAAAGTGGCCA+TGG 0.536779 4_4:+13262900 Msa0670670:three_prime_UTR
TTGTGAATATCAAATATGTC+TGG 0.542018 4_4:+13261223 Msa0670670:exon
TGTGGAAGTAGTTGCAATTG+TGG 0.548819 4_4:+13261253 Msa0670670:CDS
AATATGTCTGGCTGTAATTG+TGG 0.549280 4_4:+13261235 Msa0670670:exon
GCCCAGAATCACGGTTTCTG+TGG 0.562120 4_4:-13262658 None:intergenic
GAGGATGGTGGCTGCAAGTG+TGG 0.564753 4_4:+13262732 Msa0670670:CDS
GATGCAACAGCAGGTCTAGT+GGG 0.579454 4_4:+13262613 Msa0670670:intron
TGAAATGGGTGTTGCAGCTG+AGG 0.589074 4_4:+13262713 Msa0670670:CDS
GAAACCGTGATTCTGGGCGT+TGG 0.589097 4_4:+13262663 Msa0670670:CDS
CGGACCAACGCCCAGAATCA+CGG 0.590019 4_4:-13262667 None:intergenic
ATGGGTGTTGCAGCTGAGGA+TGG 0.598628 4_4:+13262717 Msa0670670:CDS
AGTGGGTTGGGCTATCTCGA+AGG 0.606024 4_4:+13262630 Msa0670670:CDS
AGATGCAACAGCAGGTCTAG+TGG 0.608709 4_4:+13262612 Msa0670670:intron
GGTGTTGCAGCTGAGGATGG+TGG 0.628303 4_4:+13262720 Msa0670670:CDS
ATACACACAAATACACACAT+TGG 0.647547 4_4:-13262830 None:intergenic
AGTGAAGTGCAACTTATGAA+AGG 0.648960 4_4:+13262787 Msa0670670:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAACAAAAAAAATATATA+TGG - chr4_4:13261433-13261452 None:intergenic 10.0%
!! AACAAAATATATATTATTAC+TGG - chr4_4:13262258-13262277 None:intergenic 10.0%
!! TTAATTAGAATAATATAAAG+TGG + chr4_4:13262894-13262913 Msa0670670:three_prime_UTR 10.0%
!! AGAAACAAAAAAAATATATA+TGG - chr4_4:13261433-13261452 None:intergenic 10.0%
!! AACAAAATATATATTATTAC+TGG - chr4_4:13262258-13262277 None:intergenic 10.0%
!! TTAATTAGAATAATATAAAG+TGG + chr4_4:13262894-13262913 Msa0670670:three_prime_UTR 10.0%
!! GAGAAATTATGAAAAATTAA+GGG - chr4_4:13261620-13261639 None:intergenic 15.0%
!! AGAGAAATTATGAAAAATTA+AGG - chr4_4:13261621-13261640 None:intergenic 15.0%
!!! TAATTTTTCATAATTTCTCT+AGG + chr4_4:13261621-13261640 Msa0670670:intron 15.0%
!!! GATTGAATTTCTTTTAAAAA+AGG + chr4_4:13261839-13261858 Msa0670670:intron 15.0%
!! AAGTTGTCAATATAAAAAAT+AGG + chr4_4:13261936-13261955 Msa0670670:intron 15.0%
!! AGTTGTCAATATAAAAAATA+GGG + chr4_4:13261937-13261956 Msa0670670:intron 15.0%
!!! AAAGAATCCTATTTAAAATA+TGG - chr4_4:13261988-13262007 None:intergenic 15.0%
!! TAGAATAAATAAGTAACTTT+AGG - chr4_4:13262054-13262073 None:intergenic 15.0%
!! CTAATTAAAATATTGATAAG+AGG - chr4_4:13262194-13262213 None:intergenic 15.0%
!! TCATATAAGAAAATTAGATA+AGG + chr4_4:13262217-13262236 Msa0670670:intron 15.0%
!! AAATATATATTATTACTGGA+CGG - chr4_4:13262254-13262273 None:intergenic 15.0%
!! GAGAAATTATGAAAAATTAA+GGG - chr4_4:13261620-13261639 None:intergenic 15.0%
!! AGAGAAATTATGAAAAATTA+AGG - chr4_4:13261621-13261640 None:intergenic 15.0%
!!! TAATTTTTCATAATTTCTCT+AGG + chr4_4:13261621-13261640 Msa0670670:intron 15.0%
!!! GATTGAATTTCTTTTAAAAA+AGG + chr4_4:13261839-13261858 Msa0670670:intron 15.0%
!! AAGTTGTCAATATAAAAAAT+AGG + chr4_4:13261936-13261955 Msa0670670:intron 15.0%
!! AGTTGTCAATATAAAAAATA+GGG + chr4_4:13261937-13261956 Msa0670670:intron 15.0%
!!! AAAGAATCCTATTTAAAATA+TGG - chr4_4:13261988-13262007 None:intergenic 15.0%
!! TAGAATAAATAAGTAACTTT+AGG - chr4_4:13262054-13262073 None:intergenic 15.0%
!! CTAATTAAAATATTGATAAG+AGG - chr4_4:13262194-13262213 None:intergenic 15.0%
!! TCATATAAGAAAATTAGATA+AGG + chr4_4:13262217-13262236 Msa0670670:intron 15.0%
!! AAATATATATTATTACTGGA+CGG - chr4_4:13262254-13262273 None:intergenic 15.0%
!! AGCAAAGAATAATATTTGAT+TGG - chr4_4:13261191-13261210 None:intergenic 20.0%
!! TAAAAAGCAACAAAGAATTA+AGG - chr4_4:13261305-13261324 None:intergenic 20.0%
!!! TTGTTTTTTTCATTACTCTT+TGG + chr4_4:13261381-13261400 Msa0670670:intron 20.0%
!!! TGTTTTTTTCATTACTCTTT+GGG + chr4_4:13261382-13261401 Msa0670670:intron 20.0%
!!! ATATTTTTTTTGTTTCTGCA+TGG + chr4_4:13261436-13261455 Msa0670670:intron 20.0%
!!! TATTTTTTTTGTTTCTGCAT+GGG + chr4_4:13261437-13261456 Msa0670670:intron 20.0%
!! AATAGCTCTTAAATAAGAAA+CGG - chr4_4:13261493-13261512 None:intergenic 20.0%
!! TGTATATATATATACATGCA+AGG + chr4_4:13261728-13261747 Msa0670670:intron 20.0%
!! GTATATATATATACATGCAA+GGG + chr4_4:13261729-13261748 Msa0670670:intron 20.0%
!!! AAAATCAGCTTTTTTTTTTG+TGG - chr4_4:13261773-13261792 None:intergenic 20.0%
!!! AACTCATCCATATTTTAAAT+AGG + chr4_4:13261978-13261997 Msa0670670:intron 20.0%
!! AATATGCAAAACATAATCTA+AGG - chr4_4:13262078-13262097 None:intergenic 20.0%
!! AGCAAAGAATAATATTTGAT+TGG - chr4_4:13261191-13261210 None:intergenic 20.0%
!! TAAAAAGCAACAAAGAATTA+AGG - chr4_4:13261305-13261324 None:intergenic 20.0%
!!! TTGTTTTTTTCATTACTCTT+TGG + chr4_4:13261381-13261400 Msa0670670:intron 20.0%
!!! TGTTTTTTTCATTACTCTTT+GGG + chr4_4:13261382-13261401 Msa0670670:intron 20.0%
!!! ATATTTTTTTTGTTTCTGCA+TGG + chr4_4:13261436-13261455 Msa0670670:intron 20.0%
!!! TATTTTTTTTGTTTCTGCAT+GGG + chr4_4:13261437-13261456 Msa0670670:intron 20.0%
!! AATAGCTCTTAAATAAGAAA+CGG - chr4_4:13261493-13261512 None:intergenic 20.0%
!! TGTATATATATATACATGCA+AGG + chr4_4:13261728-13261747 Msa0670670:intron 20.0%
!! GTATATATATATACATGCAA+GGG + chr4_4:13261729-13261748 Msa0670670:intron 20.0%
!!! AAAATCAGCTTTTTTTTTTG+TGG - chr4_4:13261773-13261792 None:intergenic 20.0%
!!! AACTCATCCATATTTTAAAT+AGG + chr4_4:13261978-13261997 Msa0670670:intron 20.0%
!! AATATGCAAAACATAATCTA+AGG - chr4_4:13262078-13262097 None:intergenic 20.0%
! TTGTGAATATCAAATATGTC+TGG + chr4_4:13261223-13261242 Msa0670670:exon 25.0%
! TGTATCTTATGAATGAATCT+TGG + chr4_4:13261338-13261357 Msa0670670:intron 25.0%
!!! ATTTTTTTTGTTTCTGCATG+GGG + chr4_4:13261438-13261457 Msa0670670:intron 25.0%
! GAGAAAAAATAGTGTTTGTT+CGG - chr4_4:13261562-13261581 None:intergenic 25.0%
!!! ATTTTGACTAAACAGTGTTT+TGG + chr4_4:13261788-13261807 Msa0670670:intron 25.0%
!!! CTTTTTTTAAAGTAACGATG+AGG + chr4_4:13262348-13262367 Msa0670670:intron 25.0%
! TTCACAATTCATGTGTATAA+TGG + chr4_4:13262574-13262593 Msa0670670:intron 25.0%
!! ATGTTCTTAGATTTTGTTGA+AGG + chr4_4:13262945-13262964 Msa0670670:three_prime_UTR 25.0%
! TTGTGAATATCAAATATGTC+TGG + chr4_4:13261223-13261242 Msa0670670:exon 25.0%
! TGTATCTTATGAATGAATCT+TGG + chr4_4:13261338-13261357 Msa0670670:intron 25.0%
!!! ATTTTTTTTGTTTCTGCATG+GGG + chr4_4:13261438-13261457 Msa0670670:intron 25.0%
! GAGAAAAAATAGTGTTTGTT+CGG - chr4_4:13261562-13261581 None:intergenic 25.0%
!!! ATTTTGACTAAACAGTGTTT+TGG + chr4_4:13261788-13261807 Msa0670670:intron 25.0%
!!! CTTTTTTTAAAGTAACGATG+AGG + chr4_4:13262348-13262367 Msa0670670:intron 25.0%
! TTCACAATTCATGTGTATAA+TGG + chr4_4:13262574-13262593 Msa0670670:intron 25.0%
!! ATGTTCTTAGATTTTGTTGA+AGG + chr4_4:13262945-13262964 Msa0670670:three_prime_UTR 25.0%
! TATTGATAACCACAAGTCTT+TGG + chr4_4:13261521-13261540 Msa0670670:intron 30.0%
TTGATAAATCCAAAGACTTG+TGG - chr4_4:13261533-13261552 None:intergenic 30.0%
! TCATACATGAATGAGCTTTT+TGG + chr4_4:13261653-13261672 Msa0670670:intron 30.0%
! CATACATGAATGAGCTTTTT+GGG + chr4_4:13261654-13261673 Msa0670670:intron 30.0%
!!! TCAGCTTTTTTTTTTGTGGT+CGG - chr4_4:13261769-13261788 None:intergenic 30.0%
!! AGGATTCTTTTAACTGAGAA+TGG + chr4_4:13261998-13262017 Msa0670670:intron 30.0%
ATACACACAAATACACACAT+TGG - chr4_4:13262833-13262852 None:intergenic 30.0%
AGAATAATATAAAGTGGCCA+TGG + chr4_4:13262900-13262919 Msa0670670:three_prime_UTR 30.0%
! TATTGATAACCACAAGTCTT+TGG + chr4_4:13261521-13261540 Msa0670670:intron 30.0%
TTGATAAATCCAAAGACTTG+TGG - chr4_4:13261533-13261552 None:intergenic 30.0%
! TCATACATGAATGAGCTTTT+TGG + chr4_4:13261653-13261672 Msa0670670:intron 30.0%
! CATACATGAATGAGCTTTTT+GGG + chr4_4:13261654-13261673 Msa0670670:intron 30.0%
!!! TCAGCTTTTTTTTTTGTGGT+CGG - chr4_4:13261769-13261788 None:intergenic 30.0%
!! AGGATTCTTTTAACTGAGAA+TGG + chr4_4:13261998-13262017 Msa0670670:intron 30.0%
ATACACACAAATACACACAT+TGG - chr4_4:13262833-13262852 None:intergenic 30.0%
AGAATAATATAAAGTGGCCA+TGG + chr4_4:13262900-13262919 Msa0670670:three_prime_UTR 30.0%
AATATGTCTGGCTGTAATTG+TGG + chr4_4:13261235-13261254 Msa0670670:exon 35.0%
!!! ACACTATTTTTTCTCGCATC+CGG + chr4_4:13261567-13261586 Msa0670670:intron 35.0%
TCTCGTGAGTTTAGATTAGT+TGG + chr4_4:13261699-13261718 Msa0670670:intron 35.0%
TATATATACATGCAAGGGTC+TGG + chr4_4:13261734-13261753 Msa0670670:intron 35.0%
ATATATACATGCAAGGGTCT+GGG + chr4_4:13261735-13261754 Msa0670670:intron 35.0%
!!! CAGCTTTTTTTTTTGTGGTC+GGG - chr4_4:13261768-13261787 None:intergenic 35.0%
ATATTATTACTGGACGGCTA+AGG - chr4_4:13262248-13262267 None:intergenic 35.0%
ACTGTAACAACGAGGAAAAA+AGG - chr4_4:13262299-13262318 None:intergenic 35.0%
!! GCTATTTCTTGTAGAGTCTA+GGG + chr4_4:13262370-13262389 Msa0670670:intron 35.0%
AGTGAAGTGCAACTTATGAA+AGG + chr4_4:13262787-13262806 Msa0670670:exon 35.0%
!! TTTTCTGAAGATGAGATCCA+TGG - chr4_4:13262920-13262939 None:intergenic 35.0%
!!! AGATTTTGTTGAAGGTGCTA+TGG + chr4_4:13262953-13262972 Msa0670670:three_prime_UTR 35.0%
AATATGTCTGGCTGTAATTG+TGG + chr4_4:13261235-13261254 Msa0670670:exon 35.0%
!!! ACACTATTTTTTCTCGCATC+CGG + chr4_4:13261567-13261586 Msa0670670:intron 35.0%
TCTCGTGAGTTTAGATTAGT+TGG + chr4_4:13261699-13261718 Msa0670670:intron 35.0%
TATATATACATGCAAGGGTC+TGG + chr4_4:13261734-13261753 Msa0670670:intron 35.0%
ATATATACATGCAAGGGTCT+GGG + chr4_4:13261735-13261754 Msa0670670:intron 35.0%
!!! CAGCTTTTTTTTTTGTGGTC+GGG - chr4_4:13261768-13261787 None:intergenic 35.0%
ATATTATTACTGGACGGCTA+AGG - chr4_4:13262248-13262267 None:intergenic 35.0%
ACTGTAACAACGAGGAAAAA+AGG - chr4_4:13262299-13262318 None:intergenic 35.0%
!! GCTATTTCTTGTAGAGTCTA+GGG + chr4_4:13262370-13262389 Msa0670670:intron 35.0%
AGTGAAGTGCAACTTATGAA+AGG + chr4_4:13262787-13262806 Msa0670670:exon 35.0%
!! TTTTCTGAAGATGAGATCCA+TGG - chr4_4:13262920-13262939 None:intergenic 35.0%
!!! AGATTTTGTTGAAGGTGCTA+TGG + chr4_4:13262953-13262972 Msa0670670:three_prime_UTR 35.0%
TGTGGAAGTAGTTGCAATTG+TGG + chr4_4:13261253-13261272 Msa0670670:CDS 40.0%
TGTGGTGATAACTGCAAGTA+AGG + chr4_4:13261271-13261290 Msa0670670:intron 40.0%
!!! CACTATTTTTTCTCGCATCC+GGG + chr4_4:13261568-13261587 Msa0670670:intron 40.0%
TATATACATGCAAGGGTCTG+GGG + chr4_4:13261736-13261755 Msa0670670:intron 40.0%
TCCTCGTTGTTACAGTCAAA+AGG + chr4_4:13262303-13262322 Msa0670670:intron 40.0%
!! ACCTTTTGACTGTAACAACG+AGG - chr4_4:13262307-13262326 None:intergenic 40.0%
!! GGCTATTTCTTGTAGAGTCT+AGG + chr4_4:13262369-13262388 Msa0670670:intron 40.0%
!!! CATTTTGAGGGTGCTGAAAT+GGG + chr4_4:13262699-13262718 Msa0670670:CDS 40.0%
CACTTCACTTACAGTTGCAT+GGG - chr4_4:13262776-13262795 None:intergenic 40.0%
! TGCTATGGTTGCTTTGTGTT+TGG + chr4_4:13262968-13262987 Msa0670670:three_prime_UTR 40.0%
TGTGGAAGTAGTTGCAATTG+TGG + chr4_4:13261253-13261272 Msa0670670:CDS 40.0%
TGTGGTGATAACTGCAAGTA+AGG + chr4_4:13261271-13261290 Msa0670670:intron 40.0%
!!! CACTATTTTTTCTCGCATCC+GGG + chr4_4:13261568-13261587 Msa0670670:intron 40.0%
TATATACATGCAAGGGTCTG+GGG + chr4_4:13261736-13261755 Msa0670670:intron 40.0%
TCCTCGTTGTTACAGTCAAA+AGG + chr4_4:13262303-13262322 Msa0670670:intron 40.0%
!! ACCTTTTGACTGTAACAACG+AGG - chr4_4:13262307-13262326 None:intergenic 40.0%
!! GGCTATTTCTTGTAGAGTCT+AGG + chr4_4:13262369-13262388 Msa0670670:intron 40.0%
!!! CATTTTGAGGGTGCTGAAAT+GGG + chr4_4:13262699-13262718 Msa0670670:CDS 40.0%
CACTTCACTTACAGTTGCAT+GGG - chr4_4:13262776-13262795 None:intergenic 40.0%
! TGCTATGGTTGCTTTGTGTT+TGG + chr4_4:13262968-13262987 Msa0670670:three_prime_UTR 40.0%
CTCTCCAAAATTCTCTCACC+CGG - chr4_4:13261589-13261608 None:intergenic 45.0%
CATGCCGGAATAAACGCAAA+GGG - chr4_4:13262423-13262442 None:intergenic 45.0%
ACATGCCGGAATAAACGCAA+AGG - chr4_4:13262424-13262443 None:intergenic 45.0%
ACCTAATGATCCACACATGC+CGG - chr4_4:13262438-13262457 None:intergenic 45.0%
AACCACAGAAACCGTGATTC+TGG + chr4_4:13262656-13262675 Msa0670670:CDS 45.0%
ACCACAGAAACCGTGATTCT+GGG + chr4_4:13262657-13262676 Msa0670670:CDS 45.0%
! TCCGGCAAAGATCCATTTTG+AGG + chr4_4:13262686-13262705 Msa0670670:CDS 45.0%
CCCTCAAAATGGATCTTTGC+CGG - chr4_4:13262690-13262709 None:intergenic 45.0%
! CCGGCAAAGATCCATTTTGA+GGG + chr4_4:13262687-13262706 Msa0670670:CDS 45.0%
CCATTTCAGCACCCTCAAAA+TGG - chr4_4:13262701-13262720 None:intergenic 45.0%
!!! CCATTTTGAGGGTGCTGAAA+TGG + chr4_4:13262698-13262717 Msa0670670:CDS 45.0%
GCACTTCACTTACAGTTGCA+TGG - chr4_4:13262777-13262796 None:intergenic 45.0%
CTCTCCAAAATTCTCTCACC+CGG - chr4_4:13261589-13261608 None:intergenic 45.0%
CATGCCGGAATAAACGCAAA+GGG - chr4_4:13262423-13262442 None:intergenic 45.0%
ACATGCCGGAATAAACGCAA+AGG - chr4_4:13262424-13262443 None:intergenic 45.0%
ACCTAATGATCCACACATGC+CGG - chr4_4:13262438-13262457 None:intergenic 45.0%
AACCACAGAAACCGTGATTC+TGG + chr4_4:13262656-13262675 Msa0670670:CDS 45.0%
ACCACAGAAACCGTGATTCT+GGG + chr4_4:13262657-13262676 Msa0670670:CDS 45.0%
! TCCGGCAAAGATCCATTTTG+AGG + chr4_4:13262686-13262705 Msa0670670:CDS 45.0%
CCCTCAAAATGGATCTTTGC+CGG - chr4_4:13262690-13262709 None:intergenic 45.0%
! CCGGCAAAGATCCATTTTGA+GGG + chr4_4:13262687-13262706 Msa0670670:CDS 45.0%
CCATTTCAGCACCCTCAAAA+TGG - chr4_4:13262701-13262720 None:intergenic 45.0%
!!! CCATTTTGAGGGTGCTGAAA+TGG + chr4_4:13262698-13262717 Msa0670670:CDS 45.0%
GCACTTCACTTACAGTTGCA+TGG - chr4_4:13262777-13262796 None:intergenic 45.0%
!!! TTTTTAAAAAACAATATATA+AGG - chr4_4:13262542-13262561 None:intergenic 5.0%
!!! TTTTTAAAAAACAATATATA+AGG - chr4_4:13262542-13262561 None:intergenic 5.0%
! GCATCCGGGTGAGAGAATTT+TGG + chr4_4:13261582-13261601 Msa0670670:intron 50.0%
! TAGAGTCTAGGGTTCGAACC+AGG + chr4_4:13262381-13262400 Msa0670670:intron 50.0%
! AGAGTCTAGGGTTCGAACCA+GGG + chr4_4:13262382-13262401 Msa0670670:intron 50.0%
CGGAATAAACGCAAAGGGAG+GGG - chr4_4:13262418-13262437 None:intergenic 50.0%
CCGGAATAAACGCAAAGGGA+GGG - chr4_4:13262419-13262438 None:intergenic 50.0%
CCCTCCCTTTGCGTTTATTC+CGG + chr4_4:13262416-13262435 Msa0670670:intron 50.0%
TGCGTTTATTCCGGCATGTG+TGG + chr4_4:13262425-13262444 Msa0670670:intron 50.0%
TCCGGCATGTGTGGATCATT+AGG + chr4_4:13262434-13262453 Msa0670670:intron 50.0%
AGATGCAACAGCAGGTCTAG+TGG + chr4_4:13262612-13262631 Msa0670670:intron 50.0%
GATGCAACAGCAGGTCTAGT+GGG + chr4_4:13262613-13262632 Msa0670670:intron 50.0%
!! AACAGCAGGTCTAGTGGGTT+GGG + chr4_4:13262618-13262637 Msa0670670:CDS 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG + chr4_4:13262713-13262732 Msa0670670:CDS 50.0%
! GCATCCGGGTGAGAGAATTT+TGG + chr4_4:13261582-13261601 Msa0670670:intron 50.0%
! TAGAGTCTAGGGTTCGAACC+AGG + chr4_4:13262381-13262400 Msa0670670:intron 50.0%
! AGAGTCTAGGGTTCGAACCA+GGG + chr4_4:13262382-13262401 Msa0670670:intron 50.0%
CGGAATAAACGCAAAGGGAG+GGG - chr4_4:13262418-13262437 None:intergenic 50.0%
CCGGAATAAACGCAAAGGGA+GGG - chr4_4:13262419-13262438 None:intergenic 50.0%
CCCTCCCTTTGCGTTTATTC+CGG + chr4_4:13262416-13262435 Msa0670670:intron 50.0%
TGCGTTTATTCCGGCATGTG+TGG + chr4_4:13262425-13262444 Msa0670670:intron 50.0%
TCCGGCATGTGTGGATCATT+AGG + chr4_4:13262434-13262453 Msa0670670:intron 50.0%
AGATGCAACAGCAGGTCTAG+TGG + chr4_4:13262612-13262631 Msa0670670:intron 50.0%
GATGCAACAGCAGGTCTAGT+GGG + chr4_4:13262613-13262632 Msa0670670:intron 50.0%
!! AACAGCAGGTCTAGTGGGTT+GGG + chr4_4:13262618-13262637 Msa0670670:CDS 50.0%
! TGAAATGGGTGTTGCAGCTG+AGG + chr4_4:13262713-13262732 Msa0670670:CDS 50.0%
AAATAGGGTCGCTGCGTGTG+AGG + chr4_4:13261952-13261971 Msa0670670:intron 55.0%
GCCGGAATAAACGCAAAGGG+AGG - chr4_4:13262420-13262439 None:intergenic 55.0%
TGCCTTGCAGATGCAACAGC+AGG + chr4_4:13262604-13262623 Msa0670670:intron 55.0%
GACCTGCTGTTGCATCTGCA+AGG - chr4_4:13262609-13262628 None:intergenic 55.0%
! CAACAGCAGGTCTAGTGGGT+TGG + chr4_4:13262617-13262636 Msa0670670:CDS 55.0%
!! AGTGGGTTGGGCTATCTCGA+AGG + chr4_4:13262630-13262649 Msa0670670:CDS 55.0%
GCCCAGAATCACGGTTTCTG+TGG - chr4_4:13262661-13262680 None:intergenic 55.0%
GAAACCGTGATTCTGGGCGT+TGG + chr4_4:13262663-13262682 Msa0670670:CDS 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG + chr4_4:13262717-13262736 Msa0670670:CDS 55.0%
AAATAGGGTCGCTGCGTGTG+AGG + chr4_4:13261952-13261971 Msa0670670:intron 55.0%
GCCGGAATAAACGCAAAGGG+AGG - chr4_4:13262420-13262439 None:intergenic 55.0%
TGCCTTGCAGATGCAACAGC+AGG + chr4_4:13262604-13262623 Msa0670670:intron 55.0%
GACCTGCTGTTGCATCTGCA+AGG - chr4_4:13262609-13262628 None:intergenic 55.0%
! CAACAGCAGGTCTAGTGGGT+TGG + chr4_4:13262617-13262636 Msa0670670:CDS 55.0%
!! AGTGGGTTGGGCTATCTCGA+AGG + chr4_4:13262630-13262649 Msa0670670:CDS 55.0%
GCCCAGAATCACGGTTTCTG+TGG - chr4_4:13262661-13262680 None:intergenic 55.0%
GAAACCGTGATTCTGGGCGT+TGG + chr4_4:13262663-13262682 Msa0670670:CDS 55.0%
! ATGGGTGTTGCAGCTGAGGA+TGG + chr4_4:13262717-13262736 Msa0670670:CDS 55.0%
CGGACCAACGCCCAGAATCA+CGG - chr4_4:13262670-13262689 None:intergenic 60.0%
!! CGTGATTCTGGGCGTTGGTC+CGG + chr4_4:13262668-13262687 Msa0670670:CDS 60.0%
! GGTGTTGCAGCTGAGGATGG+TGG + chr4_4:13262720-13262739 Msa0670670:CDS 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG + chr4_4:13262732-13262751 Msa0670670:CDS 60.0%
CGGACCAACGCCCAGAATCA+CGG - chr4_4:13262670-13262689 None:intergenic 60.0%
!! CGTGATTCTGGGCGTTGGTC+CGG + chr4_4:13262668-13262687 Msa0670670:CDS 60.0%
! GGTGTTGCAGCTGAGGATGG+TGG + chr4_4:13262720-13262739 Msa0670670:CDS 60.0%
! GAGGATGGTGGCTGCAAGTG+TGG + chr4_4:13262732-13262751 Msa0670670:CDS 60.0%
! GGAGGGGAATGTGCTGACCC+TGG - chr4_4:13262402-13262421 None:intergenic 65.0%
! GGAGGGGAATGTGCTGACCC+TGG - chr4_4:13262402-13262421 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 13261162 13263048 13261162 ID=Msa0670670;Name=Msa0670670
chr4_4 mRNA 13261162 13263048 13261162 ID=Msa0670670-mRNA-1;Parent=Msa0670670;Name=Msa0670670-mRNA-1;_AED=0.27;_eAED=0.27;_QI=76|1|1|1|1|1|2|257|75
chr4_4 exon 13261162 13261287 13261162 ID=Msa0670670-mRNA-1:exon:4036;Parent=Msa0670670-mRNA-1
chr4_4 exon 13262614 13263048 13262614 ID=Msa0670670-mRNA-1:exon:4037;Parent=Msa0670670-mRNA-1
chr4_4 five_prime_UTR 13261162 13261237 13261162 ID=Msa0670670-mRNA-1:five_prime_utr;Parent=Msa0670670-mRNA-1
chr4_4 CDS 13261238 13261287 13261238 ID=Msa0670670-mRNA-1:cds;Parent=Msa0670670-mRNA-1
chr4_4 CDS 13262614 13262791 13262614 ID=Msa0670670-mRNA-1:cds;Parent=Msa0670670-mRNA-1
chr4_4 three_prime_UTR 13262792 13263048 13262792 ID=Msa0670670-mRNA-1:three_prime_utr;Parent=Msa0670670-mRNA-1
Gene Sequence

>Msa0670670

ATGTCTGGCTGTAATTGTGGAAGTAGTTGCAATTGTGGTGATAACTGCAAATGCAACAGCAGGTCTAGTGGGTTGGGCTATCTCGAAGGCGAAACCACAGAAACCGTGATTCTGGGCGTTGGTCCGGCAAAGATCCATTTTGAGGGTGCTGAAATGGGTGTTGCAGCTGAGGATGGTGGCTGCAAGTGTGGAGATAGCTGCACATGTGACCCATGCAACTGTAAGTGA
Protein sequence

>Msa0670670

MSGCNCGSSCNCGDNCKCNSRSSGLGYLEGETTETVILGVGPAKIHFEGAEMGVAAEDGGCKCGDSCTCDPCNCK*