Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0675520 | KEH21965.1 | 92.000 | 75 | 6 | 0 | 1 | 75 | 1 | 75 | 2.62e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0675520 | A0A072U7Z9 | 92.000 | 75 | 6 | 0 | 1 | 75 | 1 | 75 | 1.25e-41 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0675520 | Msa0675590 | 0.805366 | 1.381809e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0675520 | MtrunA17_Chr4g0018971 | 92.000 | 75 | 6 | 0 | 1 | 75 | 1 | 75 | 2.41e-45 | 139 |
| Msa0675520 | MtrunA17_Chr4g0018981 | 60.256 | 78 | 29 | 1 | 1 | 76 | 1 | 78 | 2.08e-27 | 94.7 |
| Msa0675520 | MtrunA17_Chr4g0019351 | 45.833 | 96 | 32 | 1 | 1 | 76 | 1 | 96 | 1.82e-19 | 75.1 |
| Msa0675520 | MtrunA17_Chr4g0018961 | 52.564 | 78 | 32 | 2 | 1 | 76 | 1 | 75 | 6.82e-18 | 70.5 |
| Msa0675520 | MtrunA17_Chr4g0019341 | 46.875 | 96 | 31 | 2 | 1 | 76 | 1 | 96 | 1.52e-17 | 70.1 |
| Msa0675520 | MtrunA17_Chr4g0019361 | 53.750 | 80 | 27 | 4 | 1 | 76 | 1 | 74 | 4.47e-17 | 68.2 |
| Msa0675520 | MtrunA17_Chr8g0368881 | 45.238 | 84 | 38 | 2 | 1 | 76 | 1 | 84 | 2.00e-16 | 67.0 |
| Msa0675520 | MtrunA17_Chr4g0018931 | 44.872 | 78 | 41 | 1 | 1 | 76 | 1 | 78 | 3.14e-16 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 7 sgRNAs with CRISPR-Local
Find 7 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTATTCATAGATAATTA+TGG | 0.237089 | 4_4:+24638349 | None:intergenic |
| CATAGATAATTATGGCAAAT+AGG | 0.317712 | 4_4:+24638357 | None:intergenic |
| TTATGGCAAATAGGAATATC+TGG | 0.326800 | 4_4:+24638366 | None:intergenic |
| AGATTTCTTCTACTCTTGCA+TGG | 0.462922 | 4_4:+24638396 | None:intergenic |
| TTTCATCAAATATATTAGCA+AGG | 0.543593 | 4_4:-24638651 | Msa0675520:CDS |
| CAAGAGTAGAAGAAATCTAG+AGG | 0.609795 | 4_4:-24638391 | Msa0675520:CDS |
| TTTCTTCTACTCTTGCATGG+AGG | 0.724829 | 4_4:+24638399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATCAAATATATTAGCA+AGG | - | chr4_4:24638414-24638433 | Msa0675520:CDS | 20.0% |
| ! | CATAGATAATTATGGCAAAT+AGG | + | chr4_4:24638711-24638730 | None:intergenic | 25.0% |
| TTATGGCAAATAGGAATATC+TGG | + | chr4_4:24638702-24638721 | None:intergenic | 30.0% | |
| !!! | CTCGCTTATTTTTGTTGTTC+TGG | - | chr4_4:24638373-24638392 | Msa0675520:CDS | 35.0% |
| AGATTTCTTCTACTCTTGCA+TGG | + | chr4_4:24638672-24638691 | None:intergenic | 35.0% | |
| CAAGAGTAGAAGAAATCTAG+AGG | - | chr4_4:24638674-24638693 | Msa0675520:CDS | 35.0% | |
| TTTCTTCTACTCTTGCATGG+AGG | + | chr4_4:24638669-24638688 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 24638356 | 24638731 | 24638356 | ID=Msa0675520;Name=Msa0675520 |
| chr4_4 | mRNA | 24638356 | 24638731 | 24638356 | ID=Msa0675520-mRNA-1;Parent=Msa0675520;Name=Msa0675520-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|76 |
| chr4_4 | exon | 24638620 | 24638731 | 24638620 | ID=Msa0675520-mRNA-1:exon:6752;Parent=Msa0675520-mRNA-1 |
| chr4_4 | exon | 24638356 | 24638474 | 24638356 | ID=Msa0675520-mRNA-1:exon:6751;Parent=Msa0675520-mRNA-1 |
| chr4_4 | CDS | 24638620 | 24638731 | 24638620 | ID=Msa0675520-mRNA-1:cds;Parent=Msa0675520-mRNA-1 |
| chr4_4 | CDS | 24638356 | 24638474 | 24638356 | ID=Msa0675520-mRNA-1:cds;Parent=Msa0675520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
