AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0675590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0675590 MtrunA17_Chr4g0018971 97.333 75 2 0 1 75 1 75 1.59e-49 150
Msa0675590 MtrunA17_Chr4g0018981 64.935 77 25 1 1 75 1 77 8.27e-31 103
Msa0675590 MtrunA17_Chr4g0019351 47.368 95 30 1 1 75 1 95 4.04e-21 79.0
Msa0675590 MtrunA17_Chr4g0018961 54.545 77 30 2 1 75 1 74 6.23e-19 73.2
Msa0675590 MtrunA17_Chr4g0019341 45.263 95 32 1 1 75 1 95 1.63e-18 72.4
Msa0675590 MtrunA17_Chr4g0019361 53.247 77 30 3 1 75 1 73 2.23e-17 68.9
Msa0675590 MtrunA17_Chr4g0018931 45.455 77 40 1 1 75 1 77 2.50e-17 68.9
Msa0675590 MtrunA17_Chr8g0368881 44.578 83 38 2 1 75 1 83 2.43e-16 66.6
Msa0675590 MtrunA17_Chr4g0019021 50.000 54 26 1 1 54 1 53 3.08e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 5 sgRNAs with CRISPR-Local

Find 4 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTATTCATTAATAATTA+TGG 0.181675 4_4:+24718292 None:intergenic
CATTAATAATTATGGCAAAT+AGG 0.239991 4_4:+24718300 None:intergenic
TGATTTCTTCTACTCTTGCA+TGG 0.446427 4_4:+24718339 None:intergenic
CAAGAGTAGAAGAAATCAAG+AGG 0.567381 4_4:-24718334 Msa0675590:CDS
TTTCTTCTACTCTTGCATGG+AGG 0.692105 4_4:+24718342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTCGCTTATTTTTGTTGTTC+TGG - chr4_4:24718319-24718338 Msa0675590:CDS 35.0%
TGATTTCTTCTACTCTTGCA+TGG + chr4_4:24718566-24718585 None:intergenic 35.0%
CAAGAGTAGAAGAAATCAAG+AGG - chr4_4:24718568-24718587 Msa0675590:CDS 35.0%
TTTCTTCTACTCTTGCATGG+AGG + chr4_4:24718563-24718582 None:intergenic 40.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 24718302 24718622 24718302 ID=Msa0675590;Name=Msa0675590
chr4_4 mRNA 24718302 24718622 24718302 ID=Msa0675590-mRNA-1;Parent=Msa0675590;Name=Msa0675590-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|75
chr4_4 exon 24718511 24718622 24718511 ID=Msa0675590-mRNA-1:exon:6768;Parent=Msa0675590-mRNA-1
chr4_4 exon 24718302 24718417 24718302 ID=Msa0675590-mRNA-1:exon:6767;Parent=Msa0675590-mRNA-1
chr4_4 CDS 24718511 24718622 24718511 ID=Msa0675590-mRNA-1:cds;Parent=Msa0675590-mRNA-1
chr4_4 CDS 24718302 24718417 24718302 ID=Msa0675590-mRNA-1:cds;Parent=Msa0675590-mRNA-1
Gene Sequence

>Msa0675590

ATGGCTTCAAAGAAATGCTCGCTTATTTTTGTTGTTCTGGTTTTTTGTATGTTGAGTTTTTCATCAAATATATTAGCAAGAAACATTGCTGATGCTTCTGAGCTTTATGCATTTTCTGATGAAAAGAGTATGAACAAGAGACTTCTTGTGCAACAAAAACGACTACCTCCATGCAAGAGTAGAAGAAATCAAGAGGAGAGAGATAATCCTATTTGCCATAATTATTAA

Protein sequence

>Msa0675590

MASKKCSLIFVVLVFCMLSFSSNILARNIADASELYAFSDEKSMNKRLLVQQKRLPPCKSRRNQEERDNPICHNY*