AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0682340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682340 MtrunA17_Chr4g0028471 99.661 590 2 0 1 590 1 590 0.0 1217
Msa0682340 MtrunA17_Chr2g0327291 89.492 590 62 0 1 590 1 590 0.0 1083
Msa0682340 MtrunA17_Chr3g0119921 43.382 544 283 6 62 584 173 712 3.00e-156 464
Msa0682340 MtrunA17_Chr7g0220881 40.179 560 290 6 62 582 152 705 7.40e-144 433
Msa0682340 MtrunA17_Chr8g0371361 41.906 556 284 9 62 582 152 703 8.39e-143 430
Msa0682340 MtrunA17_Chr1g0181461 41.667 552 285 9 66 582 2 551 3.70e-141 421
Msa0682340 MtrunA17_Chr3g0129301 29.202 589 386 9 11 574 50 632 8.33e-70 238
Msa0682340 MtrunA17_Chr3g0129301 26.316 247 149 4 64 306 433 650 1.43e-19 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0682340 AT5G60790.1 84.767 558 85 0 33 590 38 595 0.0 1011
Msa0682340 AT1G64550.1 44.587 545 276 6 62 584 173 713 7.52e-159 472
Msa0682340 AT3G54540.2 42.602 561 276 9 62 583 161 714 4.40e-152 454
Msa0682340 AT3G54540.1 42.602 561 276 9 62 583 161 714 4.40e-152 454
Msa0682340 AT5G64840.1 31.079 547 354 8 60 586 94 637 1.40e-74 251
Msa0682340 AT5G09930.1 31.047 554 353 8 46 578 69 614 3.99e-73 247
Msa0682340 AT5G09930.1 25.738 237 139 5 64 294 411 616 1.69e-15 80.5
Msa0682340 AT3G28860.1 28.856 201 128 6 81 276 1030 1220 5.65e-11 66.2
Msa0682340 AT1G28010.1 30.342 234 123 8 375 581 1006 1226 8.67e-11 65.9

Find 207 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCCAGGATTTCCTTAA+TGG 0.155402 4_4:+43715077 Msa0682340:CDS
GATAGAATAAGCATCATTTA+AGG 0.186560 4_4:-43716460 None:intergenic
GGAGAAAGAAGCTGAAATTT+TGG 0.212888 4_4:+43713634 Msa0682340:CDS
TATGGTTTACTTGGATTAAA+TGG 0.214199 4_4:+43713458 Msa0682340:CDS
GAAGAAGTTTGAACGTATTT+TGG 0.216007 4_4:+43715043 Msa0682340:CDS
TCTGAATTGGAGTTAAATTA+TGG 0.236842 4_4:+43713306 Msa0682340:CDS
GTATTATTATCAATTTCTTT+TGG 0.241222 4_4:+43716527 Msa0682340:three_prime_UTR
ATCTTCTCTTCCTCATTTCC+AGG 0.257833 4_4:-43715689 None:intergenic
AATCAGGTTTGATTCGATAT+TGG 0.285059 4_4:+43716158 Msa0682340:three_prime_UTR
CAAATGGGAGGGTGACATTA+TGG 0.294052 4_4:+43716000 Msa0682340:CDS
ATCAGGTGGCGCATGAGATA+TGG 0.300121 4_4:+43715954 Msa0682340:CDS
AGGGCTGCTATTGGGAGGTT+TGG 0.309698 4_4:+43715713 Msa0682340:CDS
CGAACGAAGGGTCGAGATTA+GGG 0.320354 4_4:-43712103 None:intergenic
GAAGGCGAAGGCGGGGATAT+TGG 0.322135 4_4:+43716039 Msa0682340:CDS
AAGGAATATATTGCTCGATT+TGG 0.324399 4_4:+43715245 Msa0682340:CDS
ATCCTCTCTCTAGAGATATT+CGG 0.334592 4_4:+43712388 Msa0682340:CDS
ACAGCCTCAATTGCTACTTC+TGG 0.335559 4_4:+43715823 Msa0682340:CDS
TGACTTTAGGCTCATTAATC+AGG 0.343599 4_4:+43715937 Msa0682340:CDS
AATCAGTGATATGAAGATTT+CGG 0.349665 4_4:+43712335 Msa0682340:CDS
CACCACCCTTTGGAGACTTA+TGG 0.350545 4_4:+43716108 Msa0682340:three_prime_UTR
CCGAACGAAGGGTCGAGATT+AGG 0.352794 4_4:-43712104 None:intergenic
CCCACTTGTACTGCTTCATC+TGG 0.353903 4_4:-43715199 None:intergenic
TCAGGTTTATCTAATAAATC+AGG 0.358920 4_4:+43716142 Msa0682340:three_prime_UTR
TATTTGTGCAGACACCATTA+AGG 0.359994 4_4:-43715091 None:intergenic
AGCAACCTTATCAGCAGCTT+TGG 0.362042 4_4:-43712307 None:intergenic
CATCATTTAAGGTTCAGTTT+AGG 0.369029 4_4:-43716449 None:intergenic
AAAGTTCTTAGCTAGTATTA+TGG 0.369516 4_4:-43716187 None:intergenic
GAGAAGATGAGGGCTGCTAT+TGG 0.370014 4_4:+43715704 Msa0682340:CDS
TGAACGTATTTATGAACGTT+TGG 0.370645 4_4:+43713776 Msa0682340:CDS
GTGCTTATCATTTAATTGCT+TGG 0.375910 4_4:+43716375 Msa0682340:three_prime_UTR
ATTGCAGATAGAGTCTTTAT+CGG 0.376053 4_4:+43713251 Msa0682340:intron
GGATGAGCCAACTAATCATT+TGG 0.377600 4_4:+43715844 Msa0682340:CDS
TCCTCTCTCTAGAGATATTC+GGG 0.379128 4_4:+43712389 Msa0682340:CDS
TTTGGTGTGGAATATATATA+TGG 0.379278 4_4:-43712040 None:intergenic
TGCTTGTTAGCCATGACTTT+AGG 0.381908 4_4:+43715924 Msa0682340:CDS
CCATCAGACAAATTCTTCAT+AGG 0.387311 4_4:-43715761 None:intergenic
TTCTTCCAAAGCTGCTGATA+AGG 0.392375 4_4:+43712302 Msa0682340:CDS
TCTCATTGTTGATTCTGAAT+TGG 0.394499 4_4:+43713293 Msa0682340:CDS
TTGACCAGCAAGATGGATAA+TGG 0.395924 4_4:+43716401 Msa0682340:three_prime_UTR
GTACTCTTTCCAGCACCATT+TGG 0.396145 4_4:-43715518 None:intergenic
GCTGAAATCTTAGATGGTCT+TGG 0.397956 4_4:+43713834 Msa0682340:CDS
TCGACCCTGCTTACTGCAAT+AGG 0.398736 4_4:+43713491 Msa0682340:CDS
TTTCTGCGGTTGATGCATCT+AGG 0.401162 4_4:-43713803 None:intergenic
CTTATGGCCTTGCTTACATC+AGG 0.402681 4_4:+43716124 Msa0682340:three_prime_UTR
AGCTGTTAGTGATGAGATAT+AGG 0.402720 4_4:+43716067 Msa0682340:three_prime_UTR
GTCTCCAAAGGGTGGTGGTT+TGG 0.403169 4_4:-43716102 None:intergenic
TGGATATTCATCACCTTAGC+AGG 0.407935 4_4:+43713543 Msa0682340:CDS
TCCAGACGTTATGGTTTACT+TGG 0.408460 4_4:+43713449 Msa0682340:intron
GGAGGCACCCACCAAGATAT+TGG 0.419291 4_4:-43716217 None:intergenic
TCTTTATCGGTTACTTTCCA+TGG 0.426012 4_4:+43713264 Msa0682340:CDS
AGAAGATGAGGGCTGCTATT+GGG 0.429033 4_4:+43715705 Msa0682340:CDS
TCAATATCCAAATGATTAGT+TGG 0.429150 4_4:-43715851 None:intergenic
CATAATGTCACCCTCCCATT+TGG 0.429695 4_4:-43715999 None:intergenic
TAAATTTGGTTCCAGGATGA+TGG 0.430173 4_4:+43713738 Msa0682340:intron
CTTAGACTCTAGGATTGCTC+TGG 0.432478 4_4:+43715490 Msa0682340:CDS
CTTTAGTACAACCAATATCT+TGG 0.432663 4_4:+43716206 Msa0682340:three_prime_UTR
ACTTCAGAAAACTGAAGAAC+AGG 0.434793 4_4:-43715410 None:intergenic
TGTCCCATTATCCATCTTGC+TGG 0.435720 4_4:-43716405 None:intergenic
CCTAATCTCGACCCTTCGTT+CGG 0.437292 4_4:+43712104 Msa0682340:five_prime_UTR
TTACTTGGATTAAATGGTTG+TGG 0.447320 4_4:+43713464 Msa0682340:CDS
TATCATAAATATAGTATTGT+CGG 0.449438 4_4:-43716494 None:intergenic
AAGGGTCGAGATTAGGGTTG+TGG 0.450903 4_4:-43712097 None:intergenic
GACTCTAGGATTGCTCTGGT+TGG 0.451696 4_4:+43715494 Msa0682340:CDS
AGCTCGCCAGGCACAGAGTA+AGG 0.453694 4_4:+43715286 Msa0682340:CDS
TGACCAGCAAGATGGATAAT+GGG 0.454508 4_4:+43716402 Msa0682340:three_prime_UTR
ATTTCGGATCGGACTTGTAC+TGG 0.457445 4_4:+43712351 Msa0682340:CDS
GGAGTATGAGTGGTGAAGTT+TGG 0.457775 4_4:-43712058 None:intergenic
AAGTTACCAAGATGATTAGT+TGG 0.459793 4_4:-43713972 None:intergenic
ATATATATATGGAGAGAGAA+TGG 0.462864 4_4:-43712029 None:intergenic
GGTTGTGATATCACATTCCC+AGG 0.464036 4_4:+43715064 Msa0682340:CDS
ACCACGTTTAGCGGCGGCGG+CGG 0.465477 4_4:-43712259 None:intergenic
AACGTTCATAAATACGTTCA+AGG 0.466725 4_4:-43713773 None:intergenic
GCATTCCTATTGCAGTAAGC+AGG 0.467187 4_4:-43713496 None:intergenic
TTAGGGTTGTGGTTTGAAAG+AGG 0.473496 4_4:-43712086 None:intergenic
TCAGGTGGCGCATGAGATAT+GGG 0.475280 4_4:+43715955 Msa0682340:CDS
AGCCTTACCACCACGTTTAG+CGG 0.476687 4_4:-43712268 None:intergenic
TGATGAAGAAAGGTTGCAAT+TGG 0.476798 4_4:+43713613 Msa0682340:CDS
TATTGAGACAATTGACTCCT+TGG 0.477061 4_4:+43715868 Msa0682340:CDS
AAGCATCTGAAGGCGAAGGC+GGG 0.478821 4_4:+43716031 Msa0682340:CDS
TAGGCCAAACCACCACCCTT+TGG 0.479157 4_4:+43716098 Msa0682340:three_prime_UTR
AGACACCATTAAGGAAATCC+TGG 0.479174 4_4:-43715082 None:intergenic
CTGGTTGGACCAAATGGTGC+TGG 0.479286 4_4:+43715509 Msa0682340:CDS
CGCCGCTAAACGTGGTGGTA+AGG 0.479944 4_4:+43712266 Msa0682340:CDS
TTGCAACCTTTCTTCATCAC+AGG 0.480984 4_4:-43713609 None:intergenic
TTCTGCGGTTGATGCATCTA+GGG 0.481859 4_4:-43713802 None:intergenic
TTGTGTTTGAATTTCGAAGA+TGG 0.481860 4_4:+43712197 Msa0682340:exon
GAAGCAGTACAAGTGGGAAC+AGG 0.482565 4_4:+43715205 Msa0682340:CDS
ATCCCACTCATTCAATGCCT+CGG 0.483892 4_4:-43715891 None:intergenic
ATTGGGAGGTTTGGACTGAC+AGG 0.484846 4_4:+43715722 Msa0682340:CDS
AGTATTCCGCTTCACTGATG+TGG 0.487881 4_4:+43715373 Msa0682340:CDS
TCTTCTCTTCCTCATTTCCA+GGG 0.489690 4_4:-43715688 None:intergenic
CGTGCTGCTGAAATCTTAGA+TGG 0.490419 4_4:+43713828 Msa0682340:CDS
ACCACCACGTTTAGCGGCGG+CGG 0.490492 4_4:-43712262 None:intergenic
GATATTGAGCAATTCGAAGA+TGG 0.490587 4_4:-43715601 None:intergenic
CTGATCAGAAAGTGACCAAA+TGG 0.491350 4_4:+43715984 Msa0682340:CDS
GGTGATTTGTTTCCTAGTGA+TGG 0.498265 4_4:+43715560 Msa0682340:CDS
AGATACATCTTTCACCTTGG+TGG 0.500738 4_4:+43716311 Msa0682340:three_prime_UTR
TATATATATGGAGAGAGAAT+GGG 0.501634 4_4:-43712028 None:intergenic
TTGGGGTTGACTTAGACTCT+AGG 0.501919 4_4:+43715480 Msa0682340:CDS
GATGAACCAACTAATCATCT+TGG 0.503837 4_4:+43713966 Msa0682340:CDS
ATTGCTCTGGTTGGACCAAA+TGG 0.504021 4_4:+43715503 Msa0682340:CDS
AGGCTTCAATTTCCCTGCTA+AGG 0.505225 4_4:-43713556 None:intergenic
ATATCCATGTGCTCAGGAAT+CGG 0.505823 4_4:-43713527 None:intergenic
TTCAGCAAAACTAGCTCGCC+AGG 0.509474 4_4:+43715274 Msa0682340:CDS
AGTGAGAGAGAAGATAGATC+TGG 0.513413 4_4:-43712156 None:intergenic
TGTTCAGACTCGTGGTGACT+TGG 0.515232 4_4:+43715169 Msa0682340:CDS
AGCCTCTGACATGTCTGCAT+TGG 0.520639 4_4:+43713574 Msa0682340:CDS
TGTGGTTTGAAAGAGGAAGA+TGG 0.522076 4_4:-43712079 None:intergenic
AGCTTTGGAAGAAGCAGCGG+CGG 0.524523 4_4:-43712292 None:intergenic
CTCATCATAAACTGAAGGGC+AGG 0.530872 4_4:-43715659 None:intergenic
GCTGTTAGTGATGAGATATA+GGG 0.531494 4_4:+43716068 Msa0682340:three_prime_UTR
CAACAATGAGATCATGTCCA+TGG 0.531598 4_4:-43713281 None:intergenic
TATATTGCTCGATTTGGTCA+TGG 0.531607 4_4:+43715251 Msa0682340:CDS
GACAATTGACTCCTTGGCCG+AGG 0.534952 4_4:+43715874 Msa0682340:CDS
GTATTCCGCTTCACTGATGT+GGG 0.537969 4_4:+43715374 Msa0682340:CDS
AGTCGGTTCCGATGAAAAGA+TGG 0.538929 4_4:-43712129 None:intergenic
AGAAAACTGAAGAACAGGAG+GGG 0.540542 4_4:-43715405 None:intergenic
AAAAGATGGAGCCGAACGAA+GGG 0.542159 4_4:-43712115 None:intergenic
AGCTTTCTTCTTGCTAGCGT+CGG 0.542244 4_4:-43712223 None:intergenic
TGATGAATATCCATGTGCTC+AGG 0.545545 4_4:-43713533 None:intergenic
AATTCTTCATAGGCATCACC+TGG 0.549166 4_4:-43715751 None:intergenic
GCTTACTGCAATAGGAATGC+GGG 0.552380 4_4:+43713499 Msa0682340:CDS
TCAGCTTCTTACTTTGCATG+TGG 0.552733 4_4:-43715118 None:intergenic
CGCTTGACCATGCCATCACT+AGG 0.553023 4_4:-43715572 None:intergenic
GTTGAGTTCAATGGCAGAGT+CGG 0.555106 4_4:+43716262 Msa0682340:three_prime_UTR
TAGTACAACCAATATCTTGG+TGG 0.557114 4_4:+43716209 Msa0682340:three_prime_UTR
ATTCACTCATCATAAACTGA+AGG 0.557664 4_4:-43715664 None:intergenic
TTTGTTTCCTAGTGATGGCA+TGG 0.558297 4_4:+43715565 Msa0682340:CDS
TTGGTTCTTGACCAGCAAGA+TGG 0.561244 4_4:+43716394 Msa0682340:three_prime_UTR
AGCAATATATTCCTTCATGT+TGG 0.561912 4_4:-43715237 None:intergenic
TTGAGATACATCTTTCACCT+TGG 0.563550 4_4:+43716308 Msa0682340:three_prime_UTR
AATTGGAGTTAAATTATGGA+AGG 0.564752 4_4:+43713310 Msa0682340:CDS
AAATTCAAACACAAACCCTA+AGG 0.565291 4_4:-43712188 None:intergenic
TCATAACCTGTGATGAAGAA+AGG 0.568129 4_4:+43713603 Msa0682340:CDS
AAGATAGATCTGGCCTAAGT+CGG 0.568371 4_4:-43712146 None:intergenic
TCATCCAGAAGTAGCAATTG+AGG 0.569015 4_4:-43715827 None:intergenic
ATAAGCACAACAAATAATAA+CGG 0.569143 4_4:-43716360 None:intergenic
ACGTTCATAAATACGTTCAA+GGG 0.570004 4_4:-43713772 None:intergenic
TGGTTCATCGAGTAGAAGAA+TGG 0.570541 4_4:-43713952 None:intergenic
TCATCGAGTAGAAGAATGGT+GGG 0.571360 4_4:-43713948 None:intergenic
AGCATCTGAAGGCGAAGGCG+GGG 0.573321 4_4:+43716032 Msa0682340:CDS
GAAAAGATGGAGCCGAACGA+AGG 0.573743 4_4:-43712116 None:intergenic
CTCATTCAATGCCTCGGCCA+AGG 0.573903 4_4:-43715885 None:intergenic
TGCTCAATATCATCAGCACT+TGG 0.575155 4_4:+43715613 Msa0682340:CDS
CTATGAAGAATTTGTCTGAT+GGG 0.576561 4_4:+43715762 Msa0682340:CDS
CAAGGCCATAAGTCTCCAAA+GGG 0.577796 4_4:-43716113 None:intergenic
GAAGCATCTGAAGGCGAAGG+CGG 0.578889 4_4:+43716030 Msa0682340:CDS
GTGGTGATTGTTGAGTTCAA+TGG 0.585033 4_4:+43716253 Msa0682340:three_prime_UTR
TGCTTACTGCAATAGGAATG+CGG 0.585830 4_4:+43713498 Msa0682340:CDS
AGTACAACCAATATCTTGGT+GGG 0.587519 4_4:+43716210 Msa0682340:three_prime_UTR
TGGCCGAGGCATTGAATGAG+TGG 0.593068 4_4:+43715888 Msa0682340:CDS
GCAAGGCCATAAGTCTCCAA+AGG 0.593440 4_4:-43716114 None:intergenic
AGATAAACCTGATGTAAGCA+AGG 0.594800 4_4:-43716131 None:intergenic
GAAATGAGGAAGAGAAGATG+AGG 0.595660 4_4:+43715693 Msa0682340:CDS
GCCGCCGCCGCCGCTAAACG+TGG 0.595826 4_4:+43712258 Msa0682340:CDS
TGATCAGAAAGTGACCAAAT+GGG 0.596562 4_4:+43715985 Msa0682340:CDS
GAAAACTGAAGAACAGGAGG+GGG 0.597589 4_4:-43715404 None:intergenic
GACACCATTAAGGAAATCCT+GGG 0.599092 4_4:-43715081 None:intergenic
ACACTGCTGAAGCTAATGAC+AGG 0.601098 4_4:+43715539 Msa0682340:CDS
CATAAGTCTCCAAAGGGTGG+TGG 0.603246 4_4:-43716107 None:intergenic
CAAGAAGCATCTGAAGGCGA+AGG 0.605026 4_4:+43716027 Msa0682340:CDS
CAGATTTGGAAGCTTGTGTG+TGG 0.605348 4_4:+43715006 Msa0682340:intron
GATTAATGAGCCTAAAGTCA+TGG 0.606985 4_4:-43715934 None:intergenic
ACTTCCGATTCCTGAGCACA+TGG 0.608657 4_4:+43713523 Msa0682340:CDS
GAGTGAATACCCTGGAAATG+AGG 0.608906 4_4:+43715679 Msa0682340:CDS
CATTCCTATTGCAGTAAGCA+GGG 0.611699 4_4:-43713495 None:intergenic
GGAGCAGATTGCCAACATGA+AGG 0.612931 4_4:+43715226 Msa0682340:CDS
GGATTTCAAGAAGCATCTGA+AGG 0.613484 4_4:+43716021 Msa0682340:CDS
ACCAGATGAAGCAGTACAAG+TGG 0.613873 4_4:+43715198 Msa0682340:CDS
AGCAGCTTTGGAAGAAGCAG+CGG 0.615495 4_4:-43712295 None:intergenic
AGAGGAAGATGGAGTATGAG+TGG 0.621019 4_4:-43712068 None:intergenic
AGAGTCGAGTAATCTTTGCA+TGG 0.621080 4_4:+43715789 Msa0682340:CDS
CCTATGAAGAATTTGTCTGA+TGG 0.621484 4_4:+43715761 Msa0682340:CDS
AATTCAAACACAAACCCTAA+GGG 0.621698 4_4:-43712187 None:intergenic
CTTTAGGCTCATTAATCAGG+TGG 0.621832 4_4:+43715940 Msa0682340:CDS
AGATGAGGGCTGCTATTGGG+AGG 0.621874 4_4:+43715708 Msa0682340:CDS
CAGAAAACTGAAGAACAGGA+GGG 0.622600 4_4:-43715406 None:intergenic
TGAGATATAGGGATAGAACT+AGG 0.625271 4_4:+43716079 Msa0682340:three_prime_UTR
TGGACTGACAGGTAAAGCCC+AGG 0.625338 4_4:+43715733 Msa0682340:CDS
GAATGAGTGGGATGGCGGCA+TGG 0.625736 4_4:+43715901 Msa0682340:CDS
CAGAAAGTGACCAAATGGGA+GGG 0.626666 4_4:+43715989 Msa0682340:CDS
GAGGCATTGAATGAGTGGGA+TGG 0.626891 4_4:+43715893 Msa0682340:CDS
TCAGAAAACTGAAGAACAGG+AGG 0.629077 4_4:-43715407 None:intergenic
TTCACTCATCATAAACTGAA+GGG 0.629235 4_4:-43715663 None:intergenic
ATTTGGTTCCAGGATGATGG+AGG 0.632914 4_4:+43713741 Msa0682340:intron
ATGAGTGGTGAAGTTTGGTG+TGG 0.634659 4_4:-43712053 None:intergenic
TGGTTCCAGGATGATGGAGG+TGG 0.635014 4_4:+43713744 Msa0682340:intron
TTCATCGAGTAGAAGAATGG+TGG 0.635101 4_4:-43713949 None:intergenic
AAGAAGCTGAAGATTTACAC+TGG 0.638565 4_4:+43715131 Msa0682340:CDS
GCCGCCGCCGCTAAACGTGG+TGG 0.638651 4_4:+43712261 Msa0682340:CDS
AAATGAGGAAGAGAAGATGA+GGG 0.639167 4_4:+43715694 Msa0682340:CDS
ACGAGGTGGACTCACAGAGA+AGG 0.639258 4_4:+43715331 Msa0682340:CDS
GTGATATGAAGATTTCGGAT+CGG 0.641156 4_4:+43712340 Msa0682340:CDS
GGCCGAGGCATTGAATGAGT+GGG 0.643694 4_4:+43715889 Msa0682340:CDS
GCATTGAATGAGTGGGATGG+CGG 0.643695 4_4:+43715896 Msa0682340:CDS
GATCAATATGTTCAGACTCG+TGG 0.643969 4_4:+43715161 Msa0682340:CDS
GGATATTCATCACCTTAGCA+GGG 0.652225 4_4:+43713544 Msa0682340:CDS
TCAGAAAGTGACCAAATGGG+AGG 0.653363 4_4:+43715988 Msa0682340:CDS
GGCCATAAGTCTCCAAAGGG+TGG 0.655589 4_4:-43716110 None:intergenic
TTCCAATGCAGACATGTCAG+AGG 0.661870 4_4:-43713576 None:intergenic
ACCCGAATATCTCTAGAGAG+AGG 0.665050 4_4:-43712390 None:intergenic
CTTACCACCACGTTTAGCGG+CGG 0.666853 4_4:-43712265 None:intergenic
TCAAACACAAACCCTAAGGG+AGG 0.672946 4_4:-43712184 None:intergenic
CCAGATGAAGCAGTACAAGT+GGG 0.690738 4_4:+43715199 Msa0682340:CDS
ATTCTTCATAGGCATCACCT+GGG 0.706152 4_4:-43715750 None:intergenic
CATCGAGTAGAAGAATGGTG+GGG 0.710127 4_4:-43713947 None:intergenic
TTTATGATGAGTGAATACCC+TGG 0.737237 4_4:+43715671 Msa0682340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAGTACATAATAAAATT+TGG - chr4_4:43713381-43713400 None:intergenic 10.0%
!! ATAAACAAAGAAAAAAAAAT+AGG - chr4_4:43714208-43714227 None:intergenic 10.0%
!!! TATTATTATCAATTTCTTTT+GGG + chr4_4:43716528-43716547 Msa0682340:three_prime_UTR 10.0%
!! TAAAAAAAAAGAGTAACAAA+TGG - chr4_4:43714264-43714283 None:intergenic 15.0%
!!! TTTTTTAATGATTTGAGTTA+TGG + chr4_4:43714374-43714393 Msa0682340:intron 15.0%
!! TTTGTAACAGATTATAAAAA+AGG - chr4_4:43714462-43714481 None:intergenic 15.0%
!!! TTTTTATAATCTGTTACAAA+CGG + chr4_4:43714462-43714481 Msa0682340:intron 15.0%
!!! ATTTTGTTCAATAGTATTTA+GGG - chr4_4:43714488-43714507 None:intergenic 15.0%
!!! TATTTTGTTCAATAGTATTT+AGG - chr4_4:43714489-43714508 None:intergenic 15.0%
!! TATCATAAATATAGTATTGT+CGG - chr4_4:43716497-43716516 None:intergenic 15.0%
!!! GTATTATTATCAATTTCTTT+TGG + chr4_4:43716527-43716546 Msa0682340:three_prime_UTR 15.0%
!! CAGATTTATTGGTATAAATT+TGG + chr4_4:43712669-43712688 Msa0682340:intron 20.0%
!! TATTTGACAACTTATTTAAC+CGG - chr4_4:43712817-43712836 None:intergenic 20.0%
!!! TATGTACTTTTATTTAAACC+TGG + chr4_4:43713389-43713408 Msa0682340:intron 20.0%
!! TTGCTAACTTTGAATAAATT+TGG + chr4_4:43713724-43713743 Msa0682340:intron 20.0%
!! TGCATAATATAGACATAAAA+GGG - chr4_4:43714779-43714798 None:intergenic 20.0%
!!! CACTATCATTTTATTTTATG+CGG + chr4_4:43714797-43714816 Msa0682340:intron 20.0%
!!! ACTATCATTTTATTTTATGC+GGG + chr4_4:43714798-43714817 Msa0682340:intron 20.0%
!!! TAGTTAGTTGATATATGTAT+AGG - chr4_4:43714975-43714994 None:intergenic 20.0%
!! ATAAGCACAACAAATAATAA+CGG - chr4_4:43716363-43716382 None:intergenic 20.0%
!! TTTGGTGTGGAATATATATA+TGG - chr4_4:43712043-43712062 None:intergenic 25.0%
! AATCAGTGATATGAAGATTT+CGG + chr4_4:43712335-43712354 Msa0682340:CDS 25.0%
!! TCTGAAGTTATTTGTGATTA+TGG + chr4_4:43712522-43712541 Msa0682340:intron 25.0%
! AGTTATTTGTGATTATGGAT+TGG + chr4_4:43712527-43712546 Msa0682340:intron 25.0%
! TGATCAATATGCAGATTTAT+TGG + chr4_4:43712658-43712677 Msa0682340:intron 25.0%
! ATATAAGTTATATGAATCCG+CGG + chr4_4:43712737-43712756 Msa0682340:intron 25.0%
! AAGGTAGAAATTCTCTTAAT+TGG - chr4_4:43712874-43712893 None:intergenic 25.0%
! AATTAAGAGAATTTCTACCT+TGG + chr4_4:43712873-43712892 Msa0682340:intron 25.0%
!! TTAGTTTATTGTTGTTAGCA+TGG + chr4_4:43713024-43713043 Msa0682340:intron 25.0%
!! TAGTTTATTGTTGTTAGCAT+GGG + chr4_4:43713025-43713044 Msa0682340:intron 25.0%
!! TGATTTTGTATTAATGCGTT+TGG + chr4_4:43713220-43713239 Msa0682340:intron 25.0%
! TCTGAATTGGAGTTAAATTA+TGG + chr4_4:43713306-43713325 Msa0682340:CDS 25.0%
! AATTGGAGTTAAATTATGGA+AGG + chr4_4:43713310-43713329 Msa0682340:CDS 25.0%
! TAATAAAATTTGGCTGTACT+CGG - chr4_4:43713371-43713390 None:intergenic 25.0%
! TATGGTTTACTTGGATTAAA+TGG + chr4_4:43713458-43713477 Msa0682340:CDS 25.0%
! AATAATTTCAGACAAACAAG+AGG - chr4_4:43714017-43714036 None:intergenic 25.0%
! CACTACATTATTCAAAGTAA+TGG - chr4_4:43714517-43714536 None:intergenic 25.0%
!!! TGCTTTAACTATGTCTTTTT+TGG + chr4_4:43714654-43714673 Msa0682340:intron 25.0%
!!! ATGCTTTACTATGTCTTTTT+TGG + chr4_4:43714699-43714718 Msa0682340:intron 25.0%
! GCATAATATAGACATAAAAG+GGG - chr4_4:43714778-43714797 None:intergenic 25.0%
! GTGCATAATATAGACATAAA+AGG - chr4_4:43714780-43714799 None:intergenic 25.0%
!!! CTATCATTTTATTTTATGCG+GGG + chr4_4:43714799-43714818 Msa0682340:intron 25.0%
!!! TATCATTTTATTTTATGCGG+GGG + chr4_4:43714800-43714819 Msa0682340:intron 25.0%
! CTATTGTTTGTTTACAGATT+TGG + chr4_4:43714992-43715011 Msa0682340:intron 25.0%
! TCAATATCCAAATGATTAGT+TGG - chr4_4:43715854-43715873 None:intergenic 25.0%
! TCAGGTTTATCTAATAAATC+AGG + chr4_4:43716142-43716161 Msa0682340:three_prime_UTR 25.0%
! AAAGTTCTTAGCTAGTATTA+TGG - chr4_4:43716190-43716209 None:intergenic 25.0%
! GATAGAATAAGCATCATTTA+AGG - chr4_4:43716463-43716482 None:intergenic 25.0%
AATTCAAACACAAACCCTAA+GGG - chr4_4:43712190-43712209 None:intergenic 30.0%
AAATTCAAACACAAACCCTA+AGG - chr4_4:43712191-43712210 None:intergenic 30.0%
TTGTGTTTGAATTTCGAAGA+TGG + chr4_4:43712197-43712216 Msa0682340:exon 30.0%
AATAATTTCGTCATCATCGA+AGG - chr4_4:43712763-43712782 None:intergenic 30.0%
! TCGATGATGACGAAATTATT+AGG + chr4_4:43712763-43712782 Msa0682340:intron 30.0%
! GGCAAAAATCTTTAAGCTTT+TGG - chr4_4:43712793-43712812 None:intergenic 30.0%
!! AACTTAATGATTTGCCCTTT+TGG + chr4_4:43712848-43712867 Msa0682340:intron 30.0%
ATTCTCTTAATTGGCCAAAA+GGG - chr4_4:43712865-43712884 None:intergenic 30.0%
AATTCTCTTAATTGGCCAAA+AGG - chr4_4:43712866-43712885 None:intergenic 30.0%
TAATGGTTACATTCACAACA+TGG - chr4_4:43713105-43713124 None:intergenic 30.0%
TACCTCCAAAAAATTGGAAT+AGG - chr4_4:43713132-43713151 None:intergenic 30.0%
!!! TATTCCAATTTTTTGGAGGT+AGG + chr4_4:43713131-43713150 Msa0682340:intron 30.0%
GACTCTATCTGCAATAAAAA+CGG - chr4_4:43713246-43713265 None:intergenic 30.0%
ATTGCAGATAGAGTCTTTAT+CGG + chr4_4:43713251-43713270 Msa0682340:intron 30.0%
!! TCTCATTGTTGATTCTGAAT+TGG + chr4_4:43713293-43713312 Msa0682340:CDS 30.0%
TTACTTGGATTAAATGGTTG+TGG + chr4_4:43713464-43713483 Msa0682340:CDS 30.0%
! CTTTGAATAAATTTGGTTCC+AGG + chr4_4:43713731-43713750 Msa0682340:intron 30.0%
ACGTTCATAAATACGTTCAA+GGG - chr4_4:43713775-43713794 None:intergenic 30.0%
AACGTTCATAAATACGTTCA+AGG - chr4_4:43713776-43713795 None:intergenic 30.0%
TGAACGTATTTATGAACGTT+TGG + chr4_4:43713776-43713795 Msa0682340:CDS 30.0%
AAGTTACCAAGATGATTAGT+TGG - chr4_4:43713975-43713994 None:intergenic 30.0%
! TTTTGGGCACACATTAATTT+TGG + chr4_4:43714054-43714073 Msa0682340:intron 30.0%
!!! TATGTCTTTTTTGGTGAAGA+TGG + chr4_4:43714663-43714682 Msa0682340:intron 30.0%
CAATATTTCCATGACTGATT+GGG + chr4_4:43714847-43714866 Msa0682340:intron 30.0%
AATATTTCCATGACTGATTG+GGG + chr4_4:43714848-43714867 Msa0682340:intron 30.0%
! GAAGAAGTTTGAACGTATTT+TGG + chr4_4:43715043-43715062 Msa0682340:CDS 30.0%
AGCAATATATTCCTTCATGT+TGG - chr4_4:43715240-43715259 None:intergenic 30.0%
AAGGAATATATTGCTCGATT+TGG + chr4_4:43715245-43715264 Msa0682340:CDS 30.0%
GGAAAAAACACTAGCAAAAA+TGG + chr4_4:43715307-43715326 Msa0682340:CDS 30.0%
!!! CAGTTTTCTGAAGTAAGTTT+TGG + chr4_4:43715419-43715438 Msa0682340:CDS 30.0%
! TTTTTGTAGATGAGATTCTC+AGG - chr4_4:43715452-43715471 None:intergenic 30.0%
!! ATCTACAAAAACATCGACTT+TGG + chr4_4:43715461-43715480 Msa0682340:CDS 30.0%
!! TCTACAAAAACATCGACTTT+GGG + chr4_4:43715462-43715481 Msa0682340:CDS 30.0%
TTCACTCATCATAAACTGAA+GGG - chr4_4:43715666-43715685 None:intergenic 30.0%
ATTCACTCATCATAAACTGA+AGG - chr4_4:43715667-43715686 None:intergenic 30.0%
CTATGAAGAATTTGTCTGAT+GGG + chr4_4:43715762-43715781 Msa0682340:CDS 30.0%
! AATCAGGTTTGATTCGATAT+TGG + chr4_4:43716158-43716177 Msa0682340:three_prime_UTR 30.0%
CTTTAGTACAACCAATATCT+TGG + chr4_4:43716206-43716225 Msa0682340:three_prime_UTR 30.0%
ATCACCACCATTGAAAAAAA+TGG - chr4_4:43716241-43716260 None:intergenic 30.0%
!! GAAGTGCTATCATTTTTGTT+TGG + chr4_4:43716284-43716303 Msa0682340:three_prime_UTR 30.0%
!! TATATGTTTTAAGTCCACCA+AGG - chr4_4:43716328-43716347 None:intergenic 30.0%
GTGCTTATCATTTAATTGCT+TGG + chr4_4:43716375-43716394 Msa0682340:three_prime_UTR 30.0%
! CATCATTTAAGGTTCAGTTT+AGG - chr4_4:43716452-43716471 None:intergenic 30.0%
GTGATATGAAGATTTCGGAT+CGG + chr4_4:43712340-43712359 Msa0682340:CDS 35.0%
! ATCCTCTCTCTAGAGATATT+CGG + chr4_4:43712388-43712407 Msa0682340:CDS 35.0%
! AGCTTAAAGATTTTTGCCAC+CGG + chr4_4:43712795-43712814 Msa0682340:intron 35.0%
TTGACAACTTATTTAACCGG+TGG - chr4_4:43712814-43712833 None:intergenic 35.0%
AAATTGGAATAGGCGAGTAA+TGG - chr4_4:43713122-43713141 None:intergenic 35.0%
!!! ACTCGCCTATTCCAATTTTT+TGG + chr4_4:43713124-43713143 Msa0682340:intron 35.0%
ACAACCTACCTCCAAAAAAT+TGG - chr4_4:43713138-43713157 None:intergenic 35.0%
! TCTTTATCGGTTACTTTCCA+TGG + chr4_4:43713264-43713283 Msa0682340:CDS 35.0%
TCAAAGCAAATCGCAATCAT+TGG - chr4_4:43713423-43713442 None:intergenic 35.0%
! TGATTTTCTTCCAGACGTTA+TGG + chr4_4:43713440-43713459 Msa0682340:intron 35.0%
TCATAACCTGTGATGAAGAA+AGG + chr4_4:43713603-43713622 Msa0682340:CDS 35.0%
TGATGAAGAAAGGTTGCAAT+TGG + chr4_4:43713613-43713632 Msa0682340:CDS 35.0%
! GGAGAAAGAAGCTGAAATTT+TGG + chr4_4:43713634-43713653 Msa0682340:CDS 35.0%
! TAAATTTGGTTCCAGGATGA+TGG + chr4_4:43713738-43713757 Msa0682340:intron 35.0%
! AAGAAGACTCGTGATTTTTC+TGG + chr4_4:43713879-43713898 Msa0682340:CDS 35.0%
GATGAACCAACTAATCATCT+TGG + chr4_4:43713966-43713985 Msa0682340:CDS 35.0%
AACAAGAGGAGTTCTTTCTA+GGG - chr4_4:43714003-43714022 None:intergenic 35.0%
AAACAAGAGGAGTTCTTTCT+AGG - chr4_4:43714004-43714023 None:intergenic 35.0%
!!! TATCACCTTGTCCTGTTTTT+TGG + chr4_4:43714037-43714056 Msa0682340:intron 35.0%
!!! ATCACCTTGTCCTGTTTTTT+GGG + chr4_4:43714038-43714057 Msa0682340:intron 35.0%
TTAATGTGTGCCCAAAAAAC+AGG - chr4_4:43714051-43714070 None:intergenic 35.0%
! GCTTGTCATGTTTATGCTTT+TGG + chr4_4:43714166-43714185 Msa0682340:intron 35.0%
TACATGCAAATGATTGTGTC+TGG - chr4_4:43714578-43714597 None:intergenic 35.0%
GCAATATTTCCATGACTGAT+TGG + chr4_4:43714846-43714865 Msa0682340:intron 35.0%
TATTTGTGCAGACACCATTA+AGG - chr4_4:43715094-43715113 None:intergenic 35.0%
AAGAAGCTGAAGATTTACAC+TGG + chr4_4:43715131-43715150 Msa0682340:CDS 35.0%
!! TATATTGCTCGATTTGGTCA+TGG + chr4_4:43715251-43715270 Msa0682340:CDS 35.0%
ACTTCAGAAAACTGAAGAAC+AGG - chr4_4:43715413-43715432 None:intergenic 35.0%
!! CTACAAAAACATCGACTTTG+GGG + chr4_4:43715463-43715482 Msa0682340:CDS 35.0%
GATATTGAGCAATTCGAAGA+TGG - chr4_4:43715604-43715623 None:intergenic 35.0%
TTTATGATGAGTGAATACCC+TGG + chr4_4:43715671-43715690 Msa0682340:CDS 35.0%
AAATGAGGAAGAGAAGATGA+GGG + chr4_4:43715694-43715713 Msa0682340:CDS 35.0%
CCATCAGACAAATTCTTCAT+AGG - chr4_4:43715764-43715783 None:intergenic 35.0%
CCTATGAAGAATTTGTCTGA+TGG + chr4_4:43715761-43715780 Msa0682340:CDS 35.0%
! TATTGAGACAATTGACTCCT+TGG + chr4_4:43715868-43715887 Msa0682340:CDS 35.0%
GATTAATGAGCCTAAAGTCA+TGG - chr4_4:43715937-43715956 None:intergenic 35.0%
TGACTTTAGGCTCATTAATC+AGG + chr4_4:43715937-43715956 Msa0682340:CDS 35.0%
TGATCAGAAAGTGACCAAAT+GGG + chr4_4:43715985-43716004 Msa0682340:CDS 35.0%
AGCTGTTAGTGATGAGATAT+AGG + chr4_4:43716067-43716086 Msa0682340:three_prime_UTR 35.0%
GCTGTTAGTGATGAGATATA+GGG + chr4_4:43716068-43716087 Msa0682340:three_prime_UTR 35.0%
TGAGATATAGGGATAGAACT+AGG + chr4_4:43716079-43716098 Msa0682340:three_prime_UTR 35.0%
AGATAAACCTGATGTAAGCA+AGG - chr4_4:43716134-43716153 None:intergenic 35.0%
TAGTACAACCAATATCTTGG+TGG + chr4_4:43716209-43716228 Msa0682340:three_prime_UTR 35.0%
AGTACAACCAATATCTTGGT+GGG + chr4_4:43716210-43716229 Msa0682340:three_prime_UTR 35.0%
ACCACCATTGAAAAAAATGG+AGG - chr4_4:43716238-43716257 None:intergenic 35.0%
TTGAGATACATCTTTCACCT+TGG + chr4_4:43716308-43716327 Msa0682340:three_prime_UTR 35.0%
TGTGGTTTGAAAGAGGAAGA+TGG - chr4_4:43712082-43712101 None:intergenic 40.0%
TTAGGGTTGTGGTTTGAAAG+AGG - chr4_4:43712089-43712108 None:intergenic 40.0%
AAGATAGATCTGGCCTAAGT+CGG - chr4_4:43712149-43712168 None:intergenic 40.0%
AGTGAGAGAGAAGATAGATC+TGG - chr4_4:43712159-43712178 None:intergenic 40.0%
TTCTTCCAAAGCTGCTGATA+AGG + chr4_4:43712302-43712321 Msa0682340:CDS 40.0%
! TCCTCTCTCTAGAGATATTC+GGG + chr4_4:43712389-43712408 Msa0682340:CDS 40.0%
ATTTCTACCTTGGTTTCGTG+CGG + chr4_4:43712883-43712902 Msa0682340:intron 40.0%
!!! CGCCTATTCCAATTTTTTGG+AGG + chr4_4:43713127-43713146 Msa0682340:intron 40.0%
CAACAATGAGATCATGTCCA+TGG - chr4_4:43713284-43713303 None:intergenic 40.0%
CAATCATTGGTAAGAGTTCC+AGG - chr4_4:43713410-43713429 None:intergenic 40.0%
TCCAGACGTTATGGTTTACT+TGG + chr4_4:43713449-43713468 Msa0682340:intron 40.0%
TCCAAGTAAACCATAACGTC+TGG - chr4_4:43713453-43713472 None:intergenic 40.0%
CATTCCTATTGCAGTAAGCA+GGG - chr4_4:43713498-43713517 None:intergenic 40.0%
TGCTTACTGCAATAGGAATG+CGG + chr4_4:43713498-43713517 Msa0682340:CDS 40.0%
ATATCCATGTGCTCAGGAAT+CGG - chr4_4:43713530-43713549 None:intergenic 40.0%
TGATGAATATCCATGTGCTC+AGG - chr4_4:43713536-43713555 None:intergenic 40.0%
TGGATATTCATCACCTTAGC+AGG + chr4_4:43713543-43713562 Msa0682340:CDS 40.0%
GGATATTCATCACCTTAGCA+GGG + chr4_4:43713544-43713563 Msa0682340:CDS 40.0%
TTGCAACCTTTCTTCATCAC+AGG - chr4_4:43713612-43713631 None:intergenic 40.0%
GCTGAAATCTTAGATGGTCT+TGG + chr4_4:43713834-43713853 Msa0682340:CDS 40.0%
! AAGACTCGTGATTTTTCTGG+AGG + chr4_4:43713882-43713901 Msa0682340:CDS 40.0%
TCATCGAGTAGAAGAATGGT+GGG - chr4_4:43713951-43713970 None:intergenic 40.0%
TTCATCGAGTAGAAGAATGG+TGG - chr4_4:43713952-43713971 None:intergenic 40.0%
TGGTTCATCGAGTAGAAGAA+TGG - chr4_4:43713955-43713974 None:intergenic 40.0%
CATTCCCAGGATTTCCTTAA+TGG + chr4_4:43715077-43715096 Msa0682340:CDS 40.0%
GACACCATTAAGGAAATCCT+GGG - chr4_4:43715084-43715103 None:intergenic 40.0%
AGACACCATTAAGGAAATCC+TGG - chr4_4:43715085-43715104 None:intergenic 40.0%
TCAGCTTCTTACTTTGCATG+TGG - chr4_4:43715121-43715140 None:intergenic 40.0%
GATCAATATGTTCAGACTCG+TGG + chr4_4:43715161-43715180 Msa0682340:CDS 40.0%
!!! TTTTTTCCTTACTCTGTGCC+TGG - chr4_4:43715295-43715314 None:intergenic 40.0%
ACACTAGCAAAAATGGAACG+AGG + chr4_4:43715314-43715333 Msa0682340:CDS 40.0%
AGAAAACTGAAGAACAGGAG+GGG - chr4_4:43715408-43715427 None:intergenic 40.0%
CAGAAAACTGAAGAACAGGA+GGG - chr4_4:43715409-43715428 None:intergenic 40.0%
TCAGAAAACTGAAGAACAGG+AGG - chr4_4:43715410-43715429 None:intergenic 40.0%
!! GGTGATTTGTTTCCTAGTGA+TGG + chr4_4:43715560-43715579 Msa0682340:CDS 40.0%
TTTGTTTCCTAGTGATGGCA+TGG + chr4_4:43715565-43715584 Msa0682340:CDS 40.0%
TGCTCAATATCATCAGCACT+TGG + chr4_4:43715613-43715632 Msa0682340:CDS 40.0%
TCTTCTCTTCCTCATTTCCA+GGG - chr4_4:43715691-43715710 None:intergenic 40.0%
ATCTTCTCTTCCTCATTTCC+AGG - chr4_4:43715692-43715711 None:intergenic 40.0%
GAAATGAGGAAGAGAAGATG+AGG + chr4_4:43715693-43715712 Msa0682340:CDS 40.0%
ATTCTTCATAGGCATCACCT+GGG - chr4_4:43715753-43715772 None:intergenic 40.0%
AATTCTTCATAGGCATCACC+TGG - chr4_4:43715754-43715773 None:intergenic 40.0%
AGAGTCGAGTAATCTTTGCA+TGG + chr4_4:43715789-43715808 Msa0682340:CDS 40.0%
TCATCCAGAAGTAGCAATTG+AGG - chr4_4:43715830-43715849 None:intergenic 40.0%
GGATGAGCCAACTAATCATT+TGG + chr4_4:43715844-43715863 Msa0682340:CDS 40.0%
! TGCTTGTTAGCCATGACTTT+AGG + chr4_4:43715924-43715943 Msa0682340:CDS 40.0%
CTTTAGGCTCATTAATCAGG+TGG + chr4_4:43715940-43715959 Msa0682340:CDS 40.0%
CTGATCAGAAAGTGACCAAA+TGG + chr4_4:43715984-43716003 Msa0682340:CDS 40.0%
!! GGATTTCAAGAAGCATCTGA+AGG + chr4_4:43716021-43716040 Msa0682340:CDS 40.0%
!!! GGTGCCTCCATTTTTTTCAA+TGG + chr4_4:43716231-43716250 Msa0682340:three_prime_UTR 40.0%
!!! GCCTCCATTTTTTTCAATGG+TGG + chr4_4:43716234-43716253 Msa0682340:three_prime_UTR 40.0%
GTGGTGATTGTTGAGTTCAA+TGG + chr4_4:43716253-43716272 Msa0682340:three_prime_UTR 40.0%
AGATACATCTTTCACCTTGG+TGG + chr4_4:43716311-43716330 Msa0682340:three_prime_UTR 40.0%
TTGACCAGCAAGATGGATAA+TGG + chr4_4:43716401-43716420 Msa0682340:three_prime_UTR 40.0%
TGACCAGCAAGATGGATAAT+GGG + chr4_4:43716402-43716421 Msa0682340:three_prime_UTR 40.0%
! ATGAGTGGTGAAGTTTGGTG+TGG - chr4_4:43712056-43712075 None:intergenic 45.0%
GGAGTATGAGTGGTGAAGTT+TGG - chr4_4:43712061-43712080 None:intergenic 45.0%
AGAGGAAGATGGAGTATGAG+TGG - chr4_4:43712071-43712090 None:intergenic 45.0%
AAAAGATGGAGCCGAACGAA+GGG - chr4_4:43712118-43712137 None:intergenic 45.0%
! GTTCGGCTCCATCTTTTCAT+CGG + chr4_4:43712121-43712140 Msa0682340:five_prime_UTR 45.0%
! AGTCGGTTCCGATGAAAAGA+TGG - chr4_4:43712132-43712151 None:intergenic 45.0%
!! CTTTTCATCGGAACCGACTT+AGG + chr4_4:43712133-43712152 Msa0682340:five_prime_UTR 45.0%
! TCTCACTCTTTTCCTCCCTT+AGG + chr4_4:43712172-43712191 Msa0682340:five_prime_UTR 45.0%
! CTCACTCTTTTCCTCCCTTA+GGG + chr4_4:43712173-43712192 Msa0682340:five_prime_UTR 45.0%
TCAAACACAAACCCTAAGGG+AGG - chr4_4:43712187-43712206 None:intergenic 45.0%
AGCTTTCTTCTTGCTAGCGT+CGG - chr4_4:43712226-43712245 None:intergenic 45.0%
AGCAACCTTATCAGCAGCTT+TGG - chr4_4:43712310-43712329 None:intergenic 45.0%
ATTTCGGATCGGACTTGTAC+TGG + chr4_4:43712351-43712370 Msa0682340:CDS 45.0%
! ACCCGAATATCTCTAGAGAG+AGG - chr4_4:43712393-43712412 None:intergenic 45.0%
AGTTTAGCGAGTCTTGCTTC+TGG + chr4_4:43712970-43712989 Msa0682340:intron 45.0%
!! TGCTTCTGGCTTTATGTTGC+TGG + chr4_4:43712984-43713003 Msa0682340:intron 45.0%
!! CTGGCTTTATGTTGCTGGTT+TGG + chr4_4:43712989-43713008 Msa0682340:intron 45.0%
GCATTCCTATTGCAGTAAGC+AGG - chr4_4:43713499-43713518 None:intergenic 45.0%
GCTTACTGCAATAGGAATGC+GGG + chr4_4:43713499-43713518 Msa0682340:CDS 45.0%
AGGCTTCAATTTCCCTGCTA+AGG - chr4_4:43713559-43713578 None:intergenic 45.0%
TTCCAATGCAGACATGTCAG+AGG - chr4_4:43713579-43713598 None:intergenic 45.0%
!!! AGCTGAAATTTTGGCTGCTC+AGG + chr4_4:43713643-43713662 Msa0682340:CDS 45.0%
! ATTTGGTTCCAGGATGATGG+AGG + chr4_4:43713741-43713760 Msa0682340:intron 45.0%
! TTCTGCGGTTGATGCATCTA+GGG - chr4_4:43713805-43713824 None:intergenic 45.0%
! TTTCTGCGGTTGATGCATCT+AGG - chr4_4:43713806-43713825 None:intergenic 45.0%
! CGTGCTGCTGAAATCTTAGA+TGG + chr4_4:43713828-43713847 Msa0682340:CDS 45.0%
!! CTCGTGATTTTTCTGGAGGA+TGG + chr4_4:43713886-43713905 Msa0682340:CDS 45.0%
CATCGAGTAGAAGAATGGTG+GGG - chr4_4:43713950-43713969 None:intergenic 45.0%
TGTGCCCAAAAAACAGGACA+AGG - chr4_4:43714045-43714064 None:intergenic 45.0%
GTAACATCCCCAATCAGTCA+TGG - chr4_4:43714858-43714877 None:intergenic 45.0%
CAGATTTGGAAGCTTGTGTG+TGG + chr4_4:43715006-43715025 Msa0682340:intron 45.0%
GGTTGTGATATCACATTCCC+AGG + chr4_4:43715064-43715083 Msa0682340:CDS 45.0%
ACCAGATGAAGCAGTACAAG+TGG + chr4_4:43715198-43715217 Msa0682340:CDS 45.0%
CCAGATGAAGCAGTACAAGT+GGG + chr4_4:43715199-43715218 Msa0682340:CDS 45.0%
CTAGCAAAAATGGAACGAGG+TGG + chr4_4:43715317-43715336 Msa0682340:CDS 45.0%
AGTATTCCGCTTCACTGATG+TGG + chr4_4:43715373-43715392 Msa0682340:CDS 45.0%
GTATTCCGCTTCACTGATGT+GGG + chr4_4:43715374-43715393 Msa0682340:CDS 45.0%
! GTTTTCCCACATCAGTGAAG+CGG - chr4_4:43715382-43715401 None:intergenic 45.0%
GAAAACTGAAGAACAGGAGG+GGG - chr4_4:43715407-43715426 None:intergenic 45.0%
! TTGGGGTTGACTTAGACTCT+AGG + chr4_4:43715480-43715499 Msa0682340:CDS 45.0%
!! CTTAGACTCTAGGATTGCTC+TGG + chr4_4:43715490-43715509 Msa0682340:CDS 45.0%
ATTGCTCTGGTTGGACCAAA+TGG + chr4_4:43715503-43715522 Msa0682340:CDS 45.0%
GTACTCTTTCCAGCACCATT+TGG - chr4_4:43715521-43715540 None:intergenic 45.0%
ACACTGCTGAAGCTAATGAC+AGG + chr4_4:43715539-43715558 Msa0682340:CDS 45.0%
CTCATCATAAACTGAAGGGC+AGG - chr4_4:43715662-43715681 None:intergenic 45.0%
GAGTGAATACCCTGGAAATG+AGG + chr4_4:43715679-43715698 Msa0682340:CDS 45.0%
AGAAGATGAGGGCTGCTATT+GGG + chr4_4:43715705-43715724 Msa0682340:CDS 45.0%
! ACAGCCTCAATTGCTACTTC+TGG + chr4_4:43715823-43715842 Msa0682340:CDS 45.0%
ATCCCACTCATTCAATGCCT+CGG - chr4_4:43715894-43715913 None:intergenic 45.0%
TCAGAAAGTGACCAAATGGG+AGG + chr4_4:43715988-43716007 Msa0682340:CDS 45.0%
CAGAAAGTGACCAAATGGGA+GGG + chr4_4:43715989-43716008 Msa0682340:CDS 45.0%
CATAATGTCACCCTCCCATT+TGG - chr4_4:43716002-43716021 None:intergenic 45.0%
CAAATGGGAGGGTGACATTA+TGG + chr4_4:43716000-43716019 Msa0682340:CDS 45.0%
CAAGGCCATAAGTCTCCAAA+GGG - chr4_4:43716116-43716135 None:intergenic 45.0%
! CTTATGGCCTTGCTTACATC+AGG + chr4_4:43716124-43716143 Msa0682340:three_prime_UTR 45.0%
GTTGAGTTCAATGGCAGAGT+CGG + chr4_4:43716262-43716281 Msa0682340:three_prime_UTR 45.0%
! TTGGTTCTTGACCAGCAAGA+TGG + chr4_4:43716394-43716413 Msa0682340:three_prime_UTR 45.0%
TGTCCCATTATCCATCTTGC+TGG - chr4_4:43716408-43716427 None:intergenic 45.0%
!!! GTTTTTTTTATTTATTTTTA+TGG + chr4_4:43713700-43713719 Msa0682340:intron 5.0%
AAGGGTCGAGATTAGGGTTG+TGG - chr4_4:43712100-43712119 None:intergenic 50.0%
CGAACGAAGGGTCGAGATTA+GGG - chr4_4:43712106-43712125 None:intergenic 50.0%
CCTAATCTCGACCCTTCGTT+CGG + chr4_4:43712104-43712123 Msa0682340:five_prime_UTR 50.0%
GAAAAGATGGAGCCGAACGA+AGG - chr4_4:43712119-43712138 None:intergenic 50.0%
AGCCTTACCACCACGTTTAG+CGG - chr4_4:43712271-43712290 None:intergenic 50.0%
!! AGCAGCTTTGGAAGAAGCAG+CGG - chr4_4:43712298-43712317 None:intergenic 50.0%
GTTTGAACCGCACGAAACCA+AGG - chr4_4:43712893-43712912 None:intergenic 50.0%
ATGCTACGTACCCATCAGCT+CGG - chr4_4:43713072-43713091 None:intergenic 50.0%
! AGCTGATGGGTACGTAGCAT+GGG + chr4_4:43713072-43713091 Msa0682340:intron 50.0%
TCGACCCTGCTTACTGCAAT+AGG + chr4_4:43713491-43713510 Msa0682340:CDS 50.0%
ACTTCCGATTCCTGAGCACA+TGG + chr4_4:43713523-43713542 Msa0682340:CDS 50.0%
AGCCTCTGACATGTCTGCAT+TGG + chr4_4:43713574-43713593 Msa0682340:CDS 50.0%
! TTCAGCAGCACGCTTTTCTG+CGG - chr4_4:43713820-43713839 None:intergenic 50.0%
TGTTCAGACTCGTGGTGACT+TGG + chr4_4:43715169-43715188 Msa0682340:CDS 50.0%
CCCACTTGTACTGCTTCATC+TGG - chr4_4:43715202-43715221 None:intergenic 50.0%
GAAGCAGTACAAGTGGGAAC+AGG + chr4_4:43715205-43715224 Msa0682340:CDS 50.0%
GGAGCAGATTGCCAACATGA+AGG + chr4_4:43715226-43715245 Msa0682340:CDS 50.0%
TTCAGCAAAACTAGCTCGCC+AGG + chr4_4:43715274-43715293 Msa0682340:CDS 50.0%
!! GACTCTAGGATTGCTCTGGT+TGG + chr4_4:43715494-43715513 Msa0682340:CDS 50.0%
GAGAAGATGAGGGCTGCTAT+TGG + chr4_4:43715704-43715723 Msa0682340:CDS 50.0%
ATTGGGAGGTTTGGACTGAC+AGG + chr4_4:43715722-43715741 Msa0682340:CDS 50.0%
!! GAGGCATTGAATGAGTGGGA+TGG + chr4_4:43715893-43715912 Msa0682340:CDS 50.0%
GCATTGAATGAGTGGGATGG+CGG + chr4_4:43715896-43715915 Msa0682340:CDS 50.0%
ATCAGGTGGCGCATGAGATA+TGG + chr4_4:43715954-43715973 Msa0682340:CDS 50.0%
TCAGGTGGCGCATGAGATAT+GGG + chr4_4:43715955-43715974 Msa0682340:CDS 50.0%
!! CAAGAAGCATCTGAAGGCGA+AGG + chr4_4:43716027-43716046 Msa0682340:CDS 50.0%
CATAAGTCTCCAAAGGGTGG+TGG - chr4_4:43716110-43716129 None:intergenic 50.0%
! CACCACCCTTTGGAGACTTA+TGG + chr4_4:43716108-43716127 Msa0682340:three_prime_UTR 50.0%
GCAAGGCCATAAGTCTCCAA+AGG - chr4_4:43716117-43716136 None:intergenic 50.0%
CCGAACGAAGGGTCGAGATT+AGG - chr4_4:43712107-43712126 None:intergenic 55.0%
CTTACCACCACGTTTAGCGG+CGG - chr4_4:43712268-43712287 None:intergenic 55.0%
!! AGCTTTGGAAGAAGCAGCGG+CGG - chr4_4:43712295-43712314 None:intergenic 55.0%
TTCGTCATCATCGAAGGCCG+CGG - chr4_4:43712757-43712776 None:intergenic 55.0%
! GAGCTGATGGGTACGTAGCA+TGG + chr4_4:43713071-43713090 Msa0682340:intron 55.0%
!! TGGTTCCAGGATGATGGAGG+TGG + chr4_4:43713744-43713763 Msa0682340:intron 55.0%
CTTCACCACCTCCATCATCC+TGG - chr4_4:43713752-43713771 None:intergenic 55.0%
! ACGAGGTGGACTCACAGAGA+AGG + chr4_4:43715331-43715350 Msa0682340:CDS 55.0%
CTGGTTGGACCAAATGGTGC+TGG + chr4_4:43715509-43715528 Msa0682340:CDS 55.0%
CGCTTGACCATGCCATCACT+AGG - chr4_4:43715575-43715594 None:intergenic 55.0%
AGATGAGGGCTGCTATTGGG+AGG + chr4_4:43715708-43715727 Msa0682340:CDS 55.0%
AGGGCTGCTATTGGGAGGTT+TGG + chr4_4:43715713-43715732 Msa0682340:CDS 55.0%
TGGACTGACAGGTAAAGCCC+AGG + chr4_4:43715733-43715752 Msa0682340:CDS 55.0%
! GACAATTGACTCCTTGGCCG+AGG + chr4_4:43715874-43715893 Msa0682340:CDS 55.0%
CTCATTCAATGCCTCGGCCA+AGG - chr4_4:43715888-43715907 None:intergenic 55.0%
!! TGGCCGAGGCATTGAATGAG+TGG + chr4_4:43715888-43715907 Msa0682340:CDS 55.0%
!! GGCCGAGGCATTGAATGAGT+GGG + chr4_4:43715889-43715908 Msa0682340:CDS 55.0%
! GAAGCATCTGAAGGCGAAGG+CGG + chr4_4:43716030-43716049 Msa0682340:CDS 55.0%
AAGCATCTGAAGGCGAAGGC+GGG + chr4_4:43716031-43716050 Msa0682340:CDS 55.0%
TAGGCCAAACCACCACCCTT+TGG + chr4_4:43716098-43716117 Msa0682340:three_prime_UTR 55.0%
! GTCTCCAAAGGGTGGTGGTT+TGG - chr4_4:43716105-43716124 None:intergenic 55.0%
GGCCATAAGTCTCCAAAGGG+TGG - chr4_4:43716113-43716132 None:intergenic 55.0%
!! GGAGGCACCCACCAAGATAT+TGG - chr4_4:43716220-43716239 None:intergenic 55.0%
CGCCGCTAAACGTGGTGGTA+AGG + chr4_4:43712266-43712285 Msa0682340:CDS 60.0%
TGAGCGTCTAGCCGAGCTGA+TGG + chr4_4:43713058-43713077 Msa0682340:intron 60.0%
GAGCGTCTAGCCGAGCTGAT+GGG + chr4_4:43713059-43713078 Msa0682340:intron 60.0%
AGCTCGCCAGGCACAGAGTA+AGG + chr4_4:43715286-43715305 Msa0682340:CDS 60.0%
! GAATGAGTGGGATGGCGGCA+TGG + chr4_4:43715901-43715920 Msa0682340:CDS 60.0%
AGCATCTGAAGGCGAAGGCG+GGG + chr4_4:43716032-43716051 Msa0682340:CDS 60.0%
GAAGGCGAAGGCGGGGATAT+TGG + chr4_4:43716039-43716058 Msa0682340:CDS 60.0%
ACCACCACGTTTAGCGGCGG+CGG - chr4_4:43712265-43712284 None:intergenic 65.0%
ACCACGTTTAGCGGCGGCGG+CGG - chr4_4:43712262-43712281 None:intergenic 70.0%
GCCGCCGCCGCTAAACGTGG+TGG + chr4_4:43712261-43712280 Msa0682340:CDS 75.0%
! GCCGCCGCCGCCGCTAAACG+TGG + chr4_4:43712258-43712277 Msa0682340:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 43712030 43716580 43712030 ID=Msa0682340;Name=Msa0682340
chr4_4 mRNA 43712030 43716580 43712030 ID=Msa0682340-mRNA-1;Parent=Msa0682340;Name=Msa0682340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=186|1|1|1|0.8|0.66|6|494|590
chr4_4 exon 43712030 43712410 43712030 ID=Msa0682340-mRNA-1:exon:10598;Parent=Msa0682340-mRNA-1
chr4_4 exon 43713258 43713331 43713258 ID=Msa0682340-mRNA-1:exon:10599;Parent=Msa0682340-mRNA-1
chr4_4 exon 43713454 43713664 43713454 ID=Msa0682340-mRNA-1:exon:10600;Parent=Msa0682340-mRNA-1
chr4_4 exon 43713753 43713987 43713753 ID=Msa0682340-mRNA-1:exon:10601;Parent=Msa0682340-mRNA-1
chr4_4 exon 43715009 43716423 43715009 ID=Msa0682340-mRNA-1:exon:10602;Parent=Msa0682340-mRNA-1
chr4_4 exon 43716444 43716580 43716444 ID=Msa0682340-mRNA-1:exon:10603;Parent=Msa0682340-mRNA-1
chr4_4 five_prime_UTR 43712030 43712215 43712030 ID=Msa0682340-mRNA-1:five_prime_utr;Parent=Msa0682340-mRNA-1
chr4_4 CDS 43712216 43712410 43712216 ID=Msa0682340-mRNA-1:cds;Parent=Msa0682340-mRNA-1
chr4_4 CDS 43713258 43713331 43713258 ID=Msa0682340-mRNA-1:cds;Parent=Msa0682340-mRNA-1
chr4_4 CDS 43713454 43713664 43713454 ID=Msa0682340-mRNA-1:cds;Parent=Msa0682340-mRNA-1
chr4_4 CDS 43713753 43713987 43713753 ID=Msa0682340-mRNA-1:cds;Parent=Msa0682340-mRNA-1
chr4_4 CDS 43715009 43716066 43715009 ID=Msa0682340-mRNA-1:cds;Parent=Msa0682340-mRNA-1
chr4_4 three_prime_UTR 43716067 43716423 43716067 ID=Msa0682340-mRNA-1:three_prime_utr;Parent=Msa0682340-mRNA-1
chr4_4 three_prime_UTR 43716444 43716580 43716444 ID=Msa0682340-mRNA-1:three_prime_utr;Parent=Msa0682340-mRNA-1
Gene Sequence

>Msa0682340

ATGGTTTCCGACGCTAGCAAGAAGAAAGCTGCACAGAAGAAAGCCGCCGCCGCCGCTAAACGTGGTGGTAAGGCTGCCGCCGCTGCTTCTTCCAAAGCTGCTGATAAGGTTGCTGATAAAATCAGTGATATGAAGATTTCGGATCGGACTTGTACTGGTGTTCTTTGTTCTCATCCTCTCTCTAGAGATATTCGGATAGAGTCTTTATCGGTTACTTTCCATGGACATGATCTCATTGTTGATTCTGAATTGGAGTTAAATTATGGAAGACGTTATGGTTTACTTGGATTAAATGGTTGTGGAAAGTCGACCCTGCTTACTGCAATAGGAATGCGGGAACTTCCGATTCCTGAGCACATGGATATTCATCACCTTAGCAGGGAAATTGAAGCCTCTGACATGTCTGCATTGGAAGCTGTCATAACCTGTGATGAAGAAAGGTTGCAATTGGAGAAAGAAGCTGAAATTTTGGCTGCTCAGGATGATGGAGGTGGTGAAGCCCTTGAACGTATTTATGAACGTTTGGACGCCCTAGATGCATCAACCGCAGAAAAGCGTGCTGCTGAAATCTTAGATGGTCTTGGTTTTGACAAGCAGATGCAAGCAAAGAAGACTCGTGATTTTTCTGGAGGATGGAGAATGAGAATTTCATTAGCACGAGCTTTATTTATGAACCCCACCATTCTTCTACTCGATGAACCAACTAATCATCTTGATTTGGAAGCTTGTGTGTGGCTCGAAGAGAGTTTGAAGAAGTTTGAACGTATTTTGGTTGTGATATCACATTCCCAGGATTTCCTTAATGGTGTCTGCACAAATATTATCCACATGCAAAGTAAGAAGCTGAAGATTTACACTGGCAACTATGATCAATATGTTCAGACTCGTGGTGACTTGGAAGAGAACCAGATGAAGCAGTACAAGTGGGAACAGGAGCAGATTGCCAACATGAAGGAATATATTGCTCGATTTGGTCATGGTTCAGCAAAACTAGCTCGCCAGGCACAGAGTAAGGAAAAAACACTAGCAAAAATGGAACGAGGTGGACTCACAGAGAAGGTAGTCAGAGATCAAGTTTTAGTATTCCGCTTCACTGATGTGGGAAAACTTCCCCCTCCTGTTCTTCAGTTTTCTGAAGTAAGTTTTGGTTACACTCCTGAGAATCTCATCTACAAAAACATCGACTTTGGGGTTGACTTAGACTCTAGGATTGCTCTGGTTGGACCAAATGGTGCTGGAAAGAGTACACTGCTGAAGCTAATGACAGGTGATTTGTTTCCTAGTGATGGCATGGTCAAGCGTCATAACCATCTTCGAATTGCTCAATATCATCAGCACTTGGCTGAAAAGTTAGACTTAGATGTGCCTGCCCTTCAGTTTATGATGAGTGAATACCCTGGAAATGAGGAAGAGAAGATGAGGGCTGCTATTGGGAGGTTTGGACTGACAGGTAAAGCCCAGGTGATGCCTATGAAGAATTTGTCTGATGGGCAAAAGAGTCGAGTAATCTTTGCATGGTTAGCTTATAGACAGCCTCAATTGCTACTTCTGGATGAGCCAACTAATCATTTGGATATTGAGACAATTGACTCCTTGGCCGAGGCATTGAATGAGTGGGATGGCGGCATGGTGCTTGTTAGCCATGACTTTAGGCTCATTAATCAGGTGGCGCATGAGATATGGGTGTGTGCTGATCAGAAAGTGACCAAATGGGAGGGTGACATTATGGATTTCAAGAAGCATCTGAAGGCGAAGGCGGGGATATTGGACTGA
Protein sequence

>Msa0682340

MVSDASKKKAAQKKAAAAAKRGGKAAAAASSKAADKVADKISDMKISDRTCTGVLCSHPLSRDIRIESLSVTFHGHDLIVDSELELNYGRRYGLLGLNGCGKSTLLTAIGMRELPIPEHMDIHHLSREIEASDMSALEAVITCDEERLQLEKEAEILAAQDDGGGEALERIYERLDALDASTAEKRAAEILDGLGFDKQMQAKKTRDFSGGWRMRISLARALFMNPTILLLDEPTNHLDLEACVWLEESLKKFERILVVISHSQDFLNGVCTNIIHMQSKKLKIYTGNYDQYVQTRGDLEENQMKQYKWEQEQIANMKEYIARFGHGSAKLARQAQSKEKTLAKMERGGLTEKVVRDQVLVFRFTDVGKLPPPVLQFSEVSFGYTPENLIYKNIDFGVDLDSRIALVGPNGAGKSTLLKLMTGDLFPSDGMVKRHNHLRIAQYHQHLAEKLDLDVPALQFMMSEYPGNEEEKMRAAIGRFGLTGKAQVMPMKNLSDGQKSRVIFAWLAYRQPQLLLLDEPTNHLDIETIDSLAEALNEWDGGMVLVSHDFRLINQVAHEIWVCADQKVTKWEGDIMDFKKHLKAKAGILD*