AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0694980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694980 MtrunA17_Chr4g0046121 93.600 250 16 0 1 250 1 250 6.35e-179 491
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0694980 AT2G34050.1 73.206 209 56 0 42 250 40 248 3.08e-118 338

Find 87 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCGGTTGATGGAATTTC+TGG 0.191016 4_4:-64386305 None:intergenic
TATCTTCAACCTCCAGAATT+TGG 0.194257 4_4:+64386190 Msa0694980:CDS
ATTGAAAATGGTTACAAAAT+AGG 0.236464 4_4:-64388906 None:intergenic
AGGAAAAGGAGGAGACAAAA+AGG 0.258067 4_4:+64389007 Msa0694980:three_prime_UTR
GGTTTGAGCTAAGCGGAGTT+GGG 0.296979 4_4:-64386077 None:intergenic
TGCTATGGTTCTCCAAATTC+TGG 0.304753 4_4:-64386202 None:intergenic
GCGAGGATAAGTTGGGAATA+AGG 0.305810 4_4:+64388987 Msa0694980:three_prime_UTR
AGAAGCTGTCCAATTGAAAA+TGG 0.323271 4_4:-64388918 None:intergenic
TGTTTAGAGCGAGGATAAGT+TGG 0.323761 4_4:+64388979 Msa0694980:three_prime_UTR
CGTGGCGTGGCCGGTTAGAC+GGG 0.343054 4_4:-64386099 None:intergenic
CTGAAAATATCACTTCCATT+TGG 0.350306 4_4:+64386460 Msa0694980:CDS
TGCAGGGGAGTTGCGGTTGA+TGG 0.354027 4_4:-64386314 None:intergenic
GCGTGGCGTGGCCGGTTAGA+CGG 0.357308 4_4:-64386100 None:intergenic
TGAAAATATCACTTCCATTT+GGG 0.358159 4_4:+64386461 Msa0694980:CDS
GTTTGAGCTAAGCGGAGTTG+GGG 0.374585 4_4:-64386076 None:intergenic
ACCCCATTTCAGAAAATTGC+AGG 0.375367 4_4:-64386331 None:intergenic
TCCAAGTGAATTTGAGTTCA+AGG 0.383385 4_4:+64388860 Msa0694980:CDS
GTAAAATGATACAGTATAGT+AGG 0.387097 4_4:-64388679 None:intergenic
AATTTAATGAATTTGAGAAT+TGG 0.387189 4_4:-64386135 None:intergenic
CTCCGAGTCAGTCAGCTTGC+TGG 0.395915 4_4:-64388757 None:intergenic
AGCAAGTCTTTATCACTTGC+TGG 0.405466 4_4:+64386834 Msa0694980:CDS
TCCTTTGCAACTCAAGCCAT+TGG 0.422997 4_4:+64386398 Msa0694980:CDS
GGGTTTGAGCTAAGCGGAGT+TGG 0.428148 4_4:-64386078 None:intergenic
CAGAAACTGTTGTGAAAGAA+TGG 0.433681 4_4:-64386269 None:intergenic
AGGGAATGTCATAGAATGTT+GGG 0.435979 4_4:+64389027 Msa0694980:three_prime_UTR
ACAAAGGAATGACAAAATAT+CGG 0.436244 4_4:-64386958 None:intergenic
CACATAGGTAGAGGTCATAT+TGG 0.436318 4_4:+64386799 Msa0694980:CDS
GTTTAGAGCGAGGATAAGTT+GGG 0.441731 4_4:+64388980 Msa0694980:three_prime_UTR
TTTGAGCTAAGCGGAGTTGG+GGG 0.449517 4_4:-64386075 None:intergenic
GTGGCGTGGCGTGGCGTGGC+CGG 0.450469 4_4:-64386108 None:intergenic
ATTCCTTTGTGGAGGGGAAG+TGG 0.451596 4_4:+64386971 Msa0694980:CDS
TTTCTGAAATGGGGTTCGCT+TGG 0.455854 4_4:+64386339 Msa0694980:CDS
TATATAACAATGTTTGTTCA+AGG 0.456491 4_4:+64386995 Msa0694980:CDS
TGACTGACTCGGAGGCGAAA+TGG 0.460162 4_4:+64388766 Msa0694980:CDS
TAGCTCAAACCCCGTCTAAC+CGG 0.460822 4_4:+64386089 Msa0694980:five_prime_UTR
TATTGAGAGGCTCAAGGATC+GGG 0.463811 4_4:+64386434 Msa0694980:CDS
TACTGGTCTTGAAGATTACA+AGG 0.470857 4_4:+64388635 Msa0694980:CDS
ATTTGCAGAGAGCAAGGACT+TGG 0.474512 4_4:+64388707 Msa0694980:CDS
CTAGAAAATGAATGTTCAGC+AGG 0.484815 4_4:-64389168 None:intergenic
AAGGGAATGTCATAGAATGT+TGG 0.484972 4_4:+64389026 Msa0694980:three_prime_UTR
AGACATTACATCATCCCAAA+TGG 0.489838 4_4:-64386475 None:intergenic
TCCTTGAACTCAAATTCACT+TGG 0.494816 4_4:-64388861 None:intergenic
TGAAAGAGTTGTTTAGAGCG+AGG 0.497712 4_4:+64388970 Msa0694980:three_prime_UTR
GGGGTTCGCTTGGATTCTGC+AGG 0.497969 4_4:+64386349 Msa0694980:CDS
ATAAGTTGGGAATAAGGAAA+AGG 0.502932 4_4:+64388993 Msa0694980:three_prime_UTR
GGCCACGCCACGCCACGCCA+CGG 0.505213 4_4:+64386110 Msa0694980:five_prime_UTR
TTGCGGTTGATGGAATTTCT+GGG 0.509138 4_4:-64386304 None:intergenic
TTTCAGTATCACATAGGTAG+AGG 0.509550 4_4:+64386790 Msa0694980:intron
CACCGTGGCGTGGCGTGGCG+TGG 0.512124 4_4:-64386112 None:intergenic
CACAGAATTTGCAGAGAGCA+AGG 0.512541 4_4:+64388701 Msa0694980:CDS
AGAAAATTGCAGGGGAGTTG+CGG 0.514104 4_4:-64386321 None:intergenic
TAAAATGATACAGTATAGTA+GGG 0.515987 4_4:-64388678 None:intergenic
ATTGGCACCGTGGCGTGGCG+TGG 0.523016 4_4:-64386117 None:intergenic
TCCAATGGCTTGAGTTGCAA+AGG 0.530974 4_4:-64386399 None:intergenic
AGAAACTGTTGTGAAAGAAT+GGG 0.537123 4_4:-64386268 None:intergenic
TTCAGCAGGAAAACAAAGAC+TGG 0.543320 4_4:-64389154 None:intergenic
TGTCATAGAATGTTGGGAGA+GGG 0.543799 4_4:+64389033 Msa0694980:three_prime_UTR
CTCGGAGGCGAAATGGCTTG+TGG 0.552401 4_4:+64388773 Msa0694980:CDS
AGTTGGGAATAAGGAAAAGG+AGG 0.554864 4_4:+64388996 Msa0694980:three_prime_UTR
CTCTCCTGATCGTACCATGA+AGG 0.560417 4_4:-64389098 None:intergenic
AGGACTTGGTACTGATCCGT+GGG 0.562824 4_4:+64388721 Msa0694980:CDS
TTTGTCATTCCTTTGTGGAG+GGG 0.566411 4_4:+64386965 Msa0694980:CDS
ATATTGAGAGGCTCAAGGAT+CGG 0.568445 4_4:+64386433 Msa0694980:CDS
CCCCATTTCAGAAAATTGCA+GGG 0.572888 4_4:-64386330 None:intergenic
AGGACGTCAAGATTCGCTAC+TGG 0.579599 4_4:+64386369 Msa0694980:CDS
TACCAGCAAGCTGACTGACT+CGG 0.584954 4_4:+64388755 Msa0694980:CDS
ATGTCATAGAATGTTGGGAG+AGG 0.587642 4_4:+64389032 Msa0694980:three_prime_UTR
CGTAGATATTGAGAGGCTCA+AGG 0.587957 4_4:+64386428 Msa0694980:CDS
TGAGAATTGGCACCGTGGCG+TGG 0.588939 4_4:-64386122 None:intergenic
TGGAGCATAGAGCTAAAGAA+TGG 0.597620 4_4:+64386854 Msa0694980:CDS
GTGGCGTGGCCGGTTAGACG+GGG 0.603940 4_4:-64386098 None:intergenic
ATTCTATCGTAGATATTGAG+AGG 0.607771 4_4:+64386421 Msa0694980:CDS
AAGGACTTGGTACTGATCCG+TGG 0.611446 4_4:+64388720 Msa0694980:CDS
TAGGCATGTTCAATGCTCGC+AGG 0.614925 4_4:-64388887 None:intergenic
CTTGAAGATTACAAGGCTAG+AGG 0.618380 4_4:+64388642 Msa0694980:CDS
ATATCTACGATAGAATCCAA+TGG 0.618401 4_4:-64386414 None:intergenic
GAATTTCTGGGAAGTTGCTG+CGG 0.624275 4_4:-64386292 None:intergenic
TATGGTTCTCCAAATTCTGG+AGG 0.630186 4_4:-64386199 None:intergenic
CCCATTTCAGAAAATTGCAG+GGG 0.636308 4_4:-64386329 None:intergenic
TTGAGCTAAGCGGAGTTGGG+GGG 0.653773 4_4:-64386074 None:intergenic
TAACCACTTCCCCTCCACAA+AGG 0.668270 4_4:-64386974 None:intergenic
CAGCAAGCTGACTGACTCGG+AGG 0.679284 4_4:+64388758 Msa0694980:CDS
TAGACGGGGTTTGAGCTAAG+CGG 0.686875 4_4:-64386084 None:intergenic
GTTGTGAAAGAATGGGCATG+AGG 0.724603 4_4:-64386261 None:intergenic
GAATTTGAGAATTGGCACCG+TGG 0.731294 4_4:-64386127 None:intergenic
TGGTAAATACAACATCCCCA+CGG 0.737738 4_4:-64388737 None:intergenic
GGACTTGGTACTGATCCGTG+GGG 0.769538 4_4:+64388722 Msa0694980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATGAATTTGAGAAT+TGG - chr4_4:64386138-64386157 None:intergenic 15.0%
!! AAAAAGGAAAGTAAAAAAAA+AGG + chr4_4:64387263-64387282 Msa0694980:intron 15.0%
!!! TAAATTTGAAGTAAAAATGT+TGG + chr4_4:64387849-64387868 Msa0694980:intron 15.0%
!! ACAACTTAAAACAAAAAAAT+TGG - chr4_4:64388207-64388226 None:intergenic 15.0%
!!! CTTAGTTTTTAACATTTTTA+TGG + chr4_4:64388323-64388342 Msa0694980:intron 15.0%
!! TAATTATATCTTGAAACAAT+TGG + chr4_4:64388378-64388397 Msa0694980:intron 15.0%
!!! GTTGTATTCAAATTATTTTT+TGG + chr4_4:64389067-64389086 Msa0694980:three_prime_UTR 15.0%
!! TATTGTTTACAAATGTTGAT+AGG + chr4_4:64386552-64386571 Msa0694980:intron 20.0%
!!! ATATTTTACATAACCTATCA+TGG - chr4_4:64386612-64386631 None:intergenic 20.0%
!!! TTGTTGTTAACTATATAACT+TGG + chr4_4:64386759-64386778 Msa0694980:intron 20.0%
!! TATATAACAATGTTTGTTCA+AGG + chr4_4:64386995-64387014 Msa0694980:CDS 20.0%
!! TTGTATCTGTAAGAATATTA+GGG - chr4_4:64387536-64387555 None:intergenic 20.0%
!! ATATTCCAAAATGATCTTAT+GGG + chr4_4:64387641-64387660 Msa0694980:intron 20.0%
!!! TCATTTAACTTTAACAAAGA+AGG + chr4_4:64387767-64387786 Msa0694980:intron 20.0%
!!! CATTTAACTTTAACAAAGAA+GGG + chr4_4:64387768-64387787 Msa0694980:intron 20.0%
!!! ATTTAACTTTAACAAAGAAG+GGG + chr4_4:64387769-64387788 Msa0694980:intron 20.0%
!! TGGATTAAAATATTTCAATC+AGG + chr4_4:64387869-64387888 Msa0694980:intron 20.0%
!!! GTTTTAAAATTCTCATTTCT+GGG - chr4_4:64388024-64388043 None:intergenic 20.0%
!!! TGTTTTAAAATTCTCATTTC+TGG - chr4_4:64388025-64388044 None:intergenic 20.0%
!!! AATTTTTATTGGCAACTATA+GGG + chr4_4:64388168-64388187 Msa0694980:intron 20.0%
!! TAAAACAAAAAAATTGGTAC+TGG - chr4_4:64388201-64388220 None:intergenic 20.0%
!! AAAATGTTAAAAACTAAGCA+TGG - chr4_4:64388321-64388340 None:intergenic 20.0%
!! TAAAATGATACAGTATAGTA+GGG - chr4_4:64388681-64388700 None:intergenic 20.0%
!! ATTGAAAATGGTTACAAAAT+AGG - chr4_4:64388909-64388928 None:intergenic 20.0%
!!! ATTTTGTAACCATTTTCAAT+TGG + chr4_4:64388909-64388928 Msa0694980:exon 20.0%
! TGAAAATATCACTTCCATTT+GGG + chr4_4:64386461-64386480 Msa0694980:CDS 25.0%
!! TTCTTTATTCCTTTTCTCTT+TGG + chr4_4:64386523-64386542 Msa0694980:intron 25.0%
! ATACAATTACCAAAGAGAAA+AGG - chr4_4:64386535-64386554 None:intergenic 25.0%
! AATTTATTTGAGTCCATGAT+AGG + chr4_4:64386596-64386615 Msa0694980:intron 25.0%
!!! GTTTTTTTTCAGTATCACAT+AGG + chr4_4:64386784-64386803 Msa0694980:intron 25.0%
! ACAAAGGAATGACAAAATAT+CGG - chr4_4:64386961-64386980 None:intergenic 25.0%
! TTTGAATAAATGACAAGAGA+GGG - chr4_4:64387035-64387054 None:intergenic 25.0%
! ATTTGAATAAATGACAAGAG+AGG - chr4_4:64387036-64387055 None:intergenic 25.0%
!! ACCCTTTTTCCATTTAAATA+TGG + chr4_4:64387127-64387146 Msa0694980:intron 25.0%
! CCCATATTTAAATGGAAAAA+GGG - chr4_4:64387131-64387150 None:intergenic 25.0%
!! CCCTTTTTCCATTTAAATAT+GGG + chr4_4:64387128-64387147 Msa0694980:intron 25.0%
! ACCCATATTTAAATGGAAAA+AGG - chr4_4:64387132-64387151 None:intergenic 25.0%
! ATACTAACAATACTAACATC+TGG - chr4_4:64387159-64387178 None:intergenic 25.0%
! AGTATTGTTAGTATTATGCT+AGG + chr4_4:64387165-64387184 Msa0694980:intron 25.0%
! GTATTGTTAGTATTATGCTA+GGG + chr4_4:64387166-64387185 Msa0694980:intron 25.0%
! TATTGTTAGTATTATGCTAG+GGG + chr4_4:64387167-64387186 Msa0694980:intron 25.0%
! AGAAATTGAAAAGTTGAAGT+GGG + chr4_4:64387235-64387254 Msa0694980:intron 25.0%
! ACATAAACAATTTCCTTGAA+TGG - chr4_4:64387289-64387308 None:intergenic 25.0%
! AAGGAAATTGTTTATGTTGT+AGG + chr4_4:64387292-64387311 Msa0694980:intron 25.0%
! AGGAAATTGTTTATGTTGTA+GGG + chr4_4:64387293-64387312 Msa0694980:intron 25.0%
! AAGTACTTCACAAAGTTTAT+TGG - chr4_4:64387430-64387449 None:intergenic 25.0%
! CTTGTATCTGTAAGAATATT+AGG - chr4_4:64387537-64387556 None:intergenic 25.0%
!!! TCAAATTTTAGTTTTCCTTC+AGG + chr4_4:64387567-64387586 Msa0694980:intron 25.0%
! CATATTCCAAAATGATCTTA+TGG + chr4_4:64387640-64387659 Msa0694980:intron 25.0%
! TATTCCAAAATGATCTTATG+GGG + chr4_4:64387642-64387661 Msa0694980:intron 25.0%
! TTATATCATATTGAGATCCA+CGG + chr4_4:64387683-64387702 Msa0694980:intron 25.0%
!! CTACAAGTTATTTAACTTCA+AGG - chr4_4:64387926-64387945 None:intergenic 25.0%
! AAACTTTAACTGATAATAGC+CGG - chr4_4:64387952-64387971 None:intergenic 25.0%
! AAAATAAAAATCACTGTAGC+AGG + chr4_4:64388095-64388114 Msa0694980:intron 25.0%
! CTATAGTTGCCAATAAAAAT+TGG - chr4_4:64388169-64388188 None:intergenic 25.0%
!! CAATTTTTATTGGCAACTAT+AGG + chr4_4:64388167-64388186 Msa0694980:intron 25.0%
!! AGTTGTTTCATATTTCCTAA+TGG + chr4_4:64388221-64388240 Msa0694980:intron 25.0%
!!! TCCTAATGGTACATATTTTT+TGG + chr4_4:64388235-64388254 Msa0694980:intron 25.0%
! TCCAAAAAATATGTACCATT+AGG - chr4_4:64388239-64388258 None:intergenic 25.0%
!! TTTTAAACCTGCTATCATAT+TGG - chr4_4:64388354-64388373 None:intergenic 25.0%
! TATCTTGAAACAATTGGAAA+TGG + chr4_4:64388384-64388403 Msa0694980:intron 25.0%
! GTAAAATGATACAGTATAGT+AGG - chr4_4:64388682-64388701 None:intergenic 25.0%
!!! CTTTTTATTTGAATGATGTG+AGG + chr4_4:64388808-64388827 Msa0694980:CDS 25.0%
!!! GAAGATTGAGATGTTTTTTC+TGG - chr4_4:64386235-64386254 None:intergenic 30.0%
AGAAACTGTTGTGAAAGAAT+GGG - chr4_4:64386271-64386290 None:intergenic 30.0%
ATATCTACGATAGAATCCAA+TGG - chr4_4:64386417-64386436 None:intergenic 30.0%
ATTCTATCGTAGATATTGAG+AGG + chr4_4:64386421-64386440 Msa0694980:CDS 30.0%
! CAAATGGAAGTGATATTTTC+AGG - chr4_4:64386462-64386481 None:intergenic 30.0%
CTGAAAATATCACTTCCATT+TGG + chr4_4:64386460-64386479 Msa0694980:CDS 30.0%
! GATATTTTGTCATTCCTTTG+TGG + chr4_4:64386960-64386979 Msa0694980:CDS 30.0%
GAGAAATTGAAAAGTTGAAG+TGG + chr4_4:64387234-64387253 Msa0694980:intron 30.0%
GTAAAAAAAAAGGCCATTCA+AGG + chr4_4:64387273-64387292 Msa0694980:intron 30.0%
ACAAAGTTTATTGGTAGACA+CGG - chr4_4:64387421-64387440 None:intergenic 30.0%
TTCTATATATCTGATCCTGA+AGG - chr4_4:64387585-64387604 None:intergenic 30.0%
ATATTTCAATCAGGAACACA+AGG + chr4_4:64387878-64387897 Msa0694980:intron 30.0%
! GTTAAATAACTTGTAGAGTC+CGG + chr4_4:64387930-64387949 Msa0694980:intron 30.0%
AAACTGTTCAAACTTCAAAG+TGG + chr4_4:64387978-64387997 Msa0694980:intron 30.0%
TAAAACATCAACAAGCCTAA+AGG + chr4_4:64388038-64388057 Msa0694980:intron 30.0%
GTTATCTTCAATGTGACTAT+GGG + chr4_4:64388136-64388155 Msa0694980:intron 30.0%
! TGATAGGTAAAAAAGGCTTT+TGG - chr4_4:64388453-64388472 None:intergenic 30.0%
!! CGAGTTTTGATAGGTAAAAA+AGG - chr4_4:64388460-64388479 None:intergenic 30.0%
! ATAAGTTGGGAATAAGGAAA+AGG + chr4_4:64388993-64389012 Msa0694980:three_prime_UTR 30.0%
!!! TTTTGGTTGTTTTTCCTTCA+TGG + chr4_4:64389084-64389103 Msa0694980:three_prime_UTR 30.0%
TATCTTCAACCTCCAGAATT+TGG + chr4_4:64386190-64386209 Msa0694980:CDS 35.0%
!!! TGAGATGTTTTTTCTGGCAT+TGG - chr4_4:64386229-64386248 None:intergenic 35.0%
CAGAAACTGTTGTGAAAGAA+TGG - chr4_4:64386272-64386291 None:intergenic 35.0%
AGACATTACATCATCCCAAA+TGG - chr4_4:64386478-64386497 None:intergenic 35.0%
!! ATAGGAGTCTATGACTTGTT+TGG + chr4_4:64386570-64386589 Msa0694980:intron 35.0%
! GTTGATTGTCTATGTTTTGC+TGG + chr4_4:64386708-64386727 Msa0694980:intron 35.0%
TTTCAGTATCACATAGGTAG+AGG + chr4_4:64386790-64386809 Msa0694980:intron 35.0%
! ATTTTGTCATTCCTTTGTGG+AGG + chr4_4:64386963-64386982 Msa0694980:CDS 35.0%
! TTTTGTCATTCCTTTGTGGA+GGG + chr4_4:64386964-64386983 Msa0694980:CDS 35.0%
TGGCGTTACCCATATTTAAA+TGG - chr4_4:64387139-64387158 None:intergenic 35.0%
TATACTACCCATATCCAAGA+AGG - chr4_4:64387211-64387230 None:intergenic 35.0%
AATTGAAAAGTTGAAGTGGG+TGG + chr4_4:64387238-64387257 Msa0694980:intron 35.0%
ATTGAAAAGTTGAAGTGGGT+GGG + chr4_4:64387239-64387258 Msa0694980:intron 35.0%
GTTTATGTTGTAGGGTTACA+TGG + chr4_4:64387301-64387320 Msa0694980:intron 35.0%
GTTACATGGAAAAGAGAAAG+CGG + chr4_4:64387315-64387334 Msa0694980:intron 35.0%
TTACATGGAAAAGAGAAAGC+GGG + chr4_4:64387316-64387335 Msa0694980:intron 35.0%
CAAAGTTTATTGGTAGACAC+GGG - chr4_4:64387420-64387439 None:intergenic 35.0%
! GGTGTTGTATGCTAAATAGA+TGG - chr4_4:64387515-64387534 None:intergenic 35.0%
CAGGATCAGATATATAGAAC+TGG + chr4_4:64387586-64387605 Msa0694980:intron 35.0%
AAAAATCTCAAGACATTCCG+TGG - chr4_4:64387703-64387722 None:intergenic 35.0%
! TTTAACTTCAAGGATAGTGC+AGG - chr4_4:64387916-64387935 None:intergenic 35.0%
AAAGTGGCCAATGTCAAAAA+TGG + chr4_4:64387994-64388013 Msa0694980:intron 35.0%
AGTCACATTGAAGATAACCA+AGG - chr4_4:64388134-64388153 None:intergenic 35.0%
GGTTATCTTCAATGTGACTA+TGG + chr4_4:64388135-64388154 Msa0694980:intron 35.0%
! GGTTTCCTGCCAATTTTTAT+TGG + chr4_4:64388157-64388176 Msa0694980:intron 35.0%
AGTTGCCAATAAAAATTGGC+AGG - chr4_4:64388165-64388184 None:intergenic 35.0%
GGTTTACATCTCAACTATCA+AGG - chr4_4:64388432-64388451 None:intergenic 35.0%
!! TGCTTTGTTCGAGTTTTGAT+AGG - chr4_4:64388469-64388488 None:intergenic 35.0%
!! TACTGGTCTTGAAGATTACA+AGG + chr4_4:64388635-64388654 Msa0694980:CDS 35.0%
! TCCAAGTGAATTTGAGTTCA+AGG + chr4_4:64388860-64388879 Msa0694980:CDS 35.0%
TCCTTGAACTCAAATTCACT+TGG - chr4_4:64388864-64388883 None:intergenic 35.0%
AGAAGCTGTCCAATTGAAAA+TGG - chr4_4:64388921-64388940 None:intergenic 35.0%
AAGGGAATGTCATAGAATGT+TGG + chr4_4:64389026-64389045 Msa0694980:three_prime_UTR 35.0%
AGGGAATGTCATAGAATGTT+GGG + chr4_4:64389027-64389046 Msa0694980:three_prime_UTR 35.0%
TATGGTTCTCCAAATTCTGG+AGG - chr4_4:64386202-64386221 None:intergenic 40.0%
TGCTATGGTTCTCCAAATTC+TGG - chr4_4:64386205-64386224 None:intergenic 40.0%
!! TTTTCTGGCATTGGATGCTA+TGG - chr4_4:64386220-64386239 None:intergenic 40.0%
!! TTGCGGTTGATGGAATTTCT+GGG - chr4_4:64386307-64386326 None:intergenic 40.0%
!! TCCCCTGCAATTTTCTGAAA+TGG + chr4_4:64386328-64386347 Msa0694980:CDS 40.0%
CCCATTTCAGAAAATTGCAG+GGG - chr4_4:64386332-64386351 None:intergenic 40.0%
!!! CCCCTGCAATTTTCTGAAAT+GGG + chr4_4:64386329-64386348 Msa0694980:CDS 40.0%
CCCCATTTCAGAAAATTGCA+GGG - chr4_4:64386333-64386352 None:intergenic 40.0%
!!! CCCTGCAATTTTCTGAAATG+GGG + chr4_4:64386330-64386349 Msa0694980:CDS 40.0%
ACCCCATTTCAGAAAATTGC+AGG - chr4_4:64386334-64386353 None:intergenic 40.0%
ATATTGAGAGGCTCAAGGAT+CGG + chr4_4:64386433-64386452 Msa0694980:CDS 40.0%
CACATAGGTAGAGGTCATAT+TGG + chr4_4:64386799-64386818 Msa0694980:CDS 40.0%
AGCAAGTCTTTATCACTTGC+TGG + chr4_4:64386834-64386853 Msa0694980:CDS 40.0%
TGGAGCATAGAGCTAAAGAA+TGG + chr4_4:64386854-64386873 Msa0694980:CDS 40.0%
GGCTGCAAAAGAATAAGCAA+AGG - chr4_4:64386940-64386959 None:intergenic 40.0%
TTTGTCATTCCTTTGTGGAG+GGG + chr4_4:64386965-64386984 Msa0694980:CDS 40.0%
ACAAATACAGACCAGCAATC+AGG - chr4_4:64387098-64387117 None:intergenic 40.0%
TGTTAGTATTATGCTAGGGG+TGG + chr4_4:64387170-64387189 Msa0694980:intron 40.0%
GTTAGTATTATGCTAGGGGT+GGG + chr4_4:64387171-64387190 Msa0694980:intron 40.0%
TTAGTATTATGCTAGGGGTG+GGG + chr4_4:64387172-64387191 Msa0694980:intron 40.0%
GATCAAACACTCAACCTTCT+TGG + chr4_4:64387194-64387213 Msa0694980:intron 40.0%
ACACTCAACCTTCTTGGATA+TGG + chr4_4:64387200-64387219 Msa0694980:intron 40.0%
CACTCAACCTTCTTGGATAT+GGG + chr4_4:64387201-64387220 Msa0694980:intron 40.0%
TTGGTAGACACGGGAAAATA+AGG - chr4_4:64387411-64387430 None:intergenic 40.0%
TGTTGCGAGTGTGATAAGAT+TGG - chr4_4:64387469-64387488 None:intergenic 40.0%
! CGATCCCCATAAGATCATTT+TGG - chr4_4:64387649-64387668 None:intergenic 40.0%
TCATGATCTGTTCGATTGCA+AGG - chr4_4:64387809-64387828 None:intergenic 40.0%
ATCGAACAGATCATGACTGA+TGG + chr4_4:64387813-64387832 Msa0694980:intron 40.0%
GGATAGTGCAGGATTTCTAT+TGG - chr4_4:64387905-64387924 None:intergenic 40.0%
!! GGGCAAACCATTTTTGACAT+TGG - chr4_4:64388004-64388023 None:intergenic 40.0%
GACTTCTATCGAACACCTTT+AGG - chr4_4:64388056-64388075 None:intergenic 40.0%
CAGGAAAAAAGTTGCTTCCT+TGG + chr4_4:64388114-64388133 Msa0694980:intron 40.0%
GGAACTTCCAATATGATAGC+AGG + chr4_4:64388344-64388363 Msa0694980:intron 40.0%
! AGACCAGTAAAAAGCATGTG+CGG - chr4_4:64388624-64388643 None:intergenic 40.0%
CTTGAAGATTACAAGGCTAG+AGG + chr4_4:64388642-64388661 Msa0694980:CDS 40.0%
TGGTAAATACAACATCCCCA+CGG - chr4_4:64388740-64388759 None:intergenic 40.0%
! TGAAAGAGTTGTTTAGAGCG+AGG + chr4_4:64388970-64388989 Msa0694980:three_prime_UTR 40.0%
! TGTTTAGAGCGAGGATAAGT+TGG + chr4_4:64388979-64388998 Msa0694980:three_prime_UTR 40.0%
! GTTTAGAGCGAGGATAAGTT+GGG + chr4_4:64388980-64388999 Msa0694980:three_prime_UTR 40.0%
AGTTGGGAATAAGGAAAAGG+AGG + chr4_4:64388996-64389015 Msa0694980:three_prime_UTR 40.0%
AGGAAAAGGAGGAGACAAAA+AGG + chr4_4:64389007-64389026 Msa0694980:three_prime_UTR 40.0%
GGAAAAGGAGGAGACAAAAA+GGG + chr4_4:64389008-64389027 Msa0694980:three_prime_UTR 40.0%
ATGTCATAGAATGTTGGGAG+AGG + chr4_4:64389032-64389051 Msa0694980:three_prime_UTR 40.0%
TGTCATAGAATGTTGGGAGA+GGG + chr4_4:64389033-64389052 Msa0694980:three_prime_UTR 40.0%
! TTTTCCTTCATGGTACGATC+AGG + chr4_4:64389094-64389113 Msa0694980:three_prime_UTR 40.0%
TTCAGCAGGAAAACAAAGAC+TGG - chr4_4:64389157-64389176 None:intergenic 40.0%
GAATTTGAGAATTGGCACCG+TGG - chr4_4:64386130-64386149 None:intergenic 45.0%
GTTGTGAAAGAATGGGCATG+AGG - chr4_4:64386264-64386283 None:intergenic 45.0%
! GAATTTCTGGGAAGTTGCTG+CGG - chr4_4:64386295-64386314 None:intergenic 45.0%
!! GTTGCGGTTGATGGAATTTC+TGG - chr4_4:64386308-64386327 None:intergenic 45.0%
AGAAAATTGCAGGGGAGTTG+CGG - chr4_4:64386324-64386343 None:intergenic 45.0%
!! TTTCTGAAATGGGGTTCGCT+TGG + chr4_4:64386339-64386358 Msa0694980:CDS 45.0%
TCCTTTGCAACTCAAGCCAT+TGG + chr4_4:64386398-64386417 Msa0694980:CDS 45.0%
! TCCAATGGCTTGAGTTGCAA+AGG - chr4_4:64386402-64386421 None:intergenic 45.0%
CGTAGATATTGAGAGGCTCA+AGG + chr4_4:64386428-64386447 Msa0694980:CDS 45.0%
TATTGAGAGGCTCAAGGATC+GGG + chr4_4:64386434-64386453 Msa0694980:CDS 45.0%
AACTGTTTGCACCTGATTGC+TGG + chr4_4:64387084-64387103 Msa0694980:intron 45.0%
GTTGAAGTGGGTGGGAAAAA+AGG + chr4_4:64387247-64387266 Msa0694980:intron 45.0%
CTTTAACAAAGAAGGGGTCG+TGG + chr4_4:64387775-64387794 Msa0694980:intron 45.0%
CACAGAATTTGCAGAGAGCA+AGG + chr4_4:64388701-64388720 Msa0694980:CDS 45.0%
ATTTGCAGAGAGCAAGGACT+TGG + chr4_4:64388707-64388726 Msa0694980:CDS 45.0%
! GCGAGGATAAGTTGGGAATA+AGG + chr4_4:64388987-64389006 Msa0694980:three_prime_UTR 45.0%
!! AATATATATTAATTAACAAA+TGG + chr4_4:64388504-64388523 Msa0694980:intron 5.0%
! TGGGGGGTGTGTTAGAGAAA+CGG - chr4_4:64386061-64386080 None:intergenic 50.0%
TTTGAGCTAAGCGGAGTTGG+GGG - chr4_4:64386078-64386097 None:intergenic 50.0%
GTTTGAGCTAAGCGGAGTTG+GGG - chr4_4:64386079-64386098 None:intergenic 50.0%
GGTTTGAGCTAAGCGGAGTT+GGG - chr4_4:64386080-64386099 None:intergenic 50.0%
TAGACGGGGTTTGAGCTAAG+CGG - chr4_4:64386087-64386106 None:intergenic 50.0%
TAGCTCAAACCCCGTCTAAC+CGG + chr4_4:64386089-64386108 Msa0694980:five_prime_UTR 50.0%
AGGACGTCAAGATTCGCTAC+TGG + chr4_4:64386369-64386388 Msa0694980:CDS 50.0%
ATTCCTTTGTGGAGGGGAAG+TGG + chr4_4:64386971-64386990 Msa0694980:CDS 50.0%
TAACCACTTCCCCTCCACAA+AGG - chr4_4:64386977-64386996 None:intergenic 50.0%
TAACAAAGAAGGGGTCGTGG+TGG + chr4_4:64387778-64387797 Msa0694980:intron 50.0%
GATCTGTTCGATTGCAAGGC+AGG - chr4_4:64387805-64387824 None:intergenic 50.0%
!! ACGCCGCACATGCTTTTTAC+TGG + chr4_4:64388618-64388637 Msa0694980:CDS 50.0%
!! AAGGACTTGGTACTGATCCG+TGG + chr4_4:64388720-64388739 Msa0694980:CDS 50.0%
!! AGGACTTGGTACTGATCCGT+GGG + chr4_4:64388721-64388740 Msa0694980:CDS 50.0%
TACCAGCAAGCTGACTGACT+CGG + chr4_4:64388755-64388774 Msa0694980:CDS 50.0%
TAGGCATGTTCAATGCTCGC+AGG - chr4_4:64388890-64388909 None:intergenic 50.0%
CTCTCCTGATCGTACCATGA+AGG - chr4_4:64389101-64389120 None:intergenic 50.0%
! GGGGGGTGTGTTAGAGAAAC+GGG - chr4_4:64386060-64386079 None:intergenic 55.0%
TTGAGCTAAGCGGAGTTGGG+GGG - chr4_4:64386077-64386096 None:intergenic 55.0%
GGGTTTGAGCTAAGCGGAGT+TGG - chr4_4:64386081-64386100 None:intergenic 55.0%
!! GGACTTGGTACTGATCCGTG+GGG + chr4_4:64388722-64388741 Msa0694980:CDS 55.0%
!! TGACTGACTCGGAGGCGAAA+TGG + chr4_4:64388766-64388785 Msa0694980:CDS 55.0%
TGAGAATTGGCACCGTGGCG+TGG - chr4_4:64386125-64386144 None:intergenic 60.0%
TGCAGGGGAGTTGCGGTTGA+TGG - chr4_4:64386317-64386336 None:intergenic 60.0%
GGGGTTCGCTTGGATTCTGC+AGG + chr4_4:64386349-64386368 Msa0694980:CDS 60.0%
CTCCGAGTCAGTCAGCTTGC+TGG - chr4_4:64388760-64388779 None:intergenic 60.0%
!! CAGCAAGCTGACTGACTCGG+AGG + chr4_4:64388758-64388777 Msa0694980:CDS 60.0%
CTCGGAGGCGAAATGGCTTG+TGG + chr4_4:64388773-64388792 Msa0694980:CDS 60.0%
GTGGCGTGGCCGGTTAGACG+GGG - chr4_4:64386101-64386120 None:intergenic 70.0%
CGTGGCGTGGCCGGTTAGAC+GGG - chr4_4:64386102-64386121 None:intergenic 70.0%
GCGTGGCGTGGCCGGTTAGA+CGG - chr4_4:64386103-64386122 None:intergenic 70.0%
ATTGGCACCGTGGCGTGGCG+TGG - chr4_4:64386120-64386139 None:intergenic 70.0%
! GTGGCGTGGCGTGGCGTGGC+CGG - chr4_4:64386111-64386130 None:intergenic 80.0%
! GGCCACGCCACGCCACGCCA+CGG + chr4_4:64386110-64386129 Msa0694980:five_prime_UTR 80.0%
! CACCGTGGCGTGGCGTGGCG+TGG - chr4_4:64386115-64386134 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64386050 64389187 64386050 ID=Msa0694980;Name=Msa0694980
chr4_4 mRNA 64386050 64389187 64386050 ID=Msa0694980-mRNA-1;Parent=Msa0694980;Name=Msa0694980-mRNA-1;_AED=0.02;_eAED=0.02;_QI=130|1|1|1|1|1|4|270|250
chr4_4 exon 64386050 64386488 64386050 ID=Msa0694980-mRNA-1:exon:18377;Parent=Msa0694980-mRNA-1
chr4_4 exon 64386796 64386875 64386796 ID=Msa0694980-mRNA-1:exon:18378;Parent=Msa0694980-mRNA-1
chr4_4 exon 64386958 64387016 64386958 ID=Msa0694980-mRNA-1:exon:18379;Parent=Msa0694980-mRNA-1
chr4_4 exon 64388613 64389187 64388613 ID=Msa0694980-mRNA-1:exon:18380;Parent=Msa0694980-mRNA-1
chr4_4 five_prime_UTR 64386050 64386179 64386050 ID=Msa0694980-mRNA-1:five_prime_utr;Parent=Msa0694980-mRNA-1
chr4_4 CDS 64386180 64386488 64386180 ID=Msa0694980-mRNA-1:cds;Parent=Msa0694980-mRNA-1
chr4_4 CDS 64386796 64386875 64386796 ID=Msa0694980-mRNA-1:cds;Parent=Msa0694980-mRNA-1
chr4_4 CDS 64386958 64387016 64386958 ID=Msa0694980-mRNA-1:cds;Parent=Msa0694980-mRNA-1
chr4_4 CDS 64388613 64388917 64388613 ID=Msa0694980-mRNA-1:cds;Parent=Msa0694980-mRNA-1
chr4_4 three_prime_UTR 64388918 64389187 64388918 ID=Msa0694980-mRNA-1:three_prime_utr;Parent=Msa0694980-mRNA-1
Gene Sequence

>Msa0694980

ATGCAAAGAGTATCTTCAACCTCCAGAATTTGGAGAACCATAGCATCCAATGCCAGAAAAAACATCTCAATCTTCAACGAACCTCATGCCCATTCTTTCACAACAGTTTCTGCCGCAGCAACTTCCCAGAAATTCCATCAACCGCAACTCCCCTGCAATTTTCTGAAATGGGGTTCGCTTGGATTCTGCAGGACGTCAAGATTCGCTACTGGCTTCAATCCTTTGCAACTCAAGCCATTGGATTCTATCGTAGATATTGAGAGGCTCAAGGATCGGGATCCTGAAAATATCACTTCCATTTGGGATGATTATCACATAGGTAGAGGTCATATTGGAGCAACTATGAAAGCAAGTCTTTATCACTTGCTGGAGCATAGAGCTAAAGAATGCCGATATTTTGTCATTCCTTTGTGGAGGGGAAGTGGTTATATAACAATGTTTGTTCAAGTTCAGACGCCGCACATGCTTTTTACTGGTCTTGAAGATTACAAGGCTAGAGGAACACAAGCATCTCCCTACTATACTGTATCATTTTACACAGAATTTGCAGAGAGCAAGGACTTGGTACTGATCCGTGGGGATGTTGTATTTACCAGCAAGCTGACTGACTCGGAGGCGAAATGGCTTGTGGAAACTGCTCAGTCTTTTTATTTGAATGATGTGAGGTACAAACTAGTCGAGAGATTCAATAAAGATCCAAGTGAATTTGAGTTCAAGGATGTCCTGCGAGCATTGAACATGCCTATTTTGTAA
Protein sequence

>Msa0694980

MQRVSSTSRIWRTIASNARKNISIFNEPHAHSFTTVSAAATSQKFHQPQLPCNFLKWGSLGFCRTSRFATGFNPLQLKPLDSIVDIERLKDRDPENITSIWDDYHIGRGHIGATMKASLYHLLEHRAKECRYFVIPLWRGSGYITMFVQVQTPHMLFTGLEDYKARGTQASPYYTVSFYTEFAESKDLVLIRGDVVFTSKLTDSEAKWLVETAQSFYLNDVRYKLVERFNKDPSEFEFKDVLRALNMPIL*