Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0698270 | Msa0698280 | 0.838339 | 3.049685e-57 | -8.615850e-47 |
| Msa0698270 | Msa1056890 | 0.806489 | 8.025525e-50 | -8.615850e-47 |
| Msa0698270 | Msa1108560 | 0.803306 | 3.711727e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTATGGTTGTTTGAATA+TGG | 0.151100 | 4_4:-68513326 | None:intergenic |
| TATTGATGAGTAAGAGGAAT+TGG | 0.362717 | 4_4:-68513220 | None:intergenic |
| GTGATAATGACAAGGGGTTT+TGG | 0.366819 | 4_4:-68513288 | None:intergenic |
| TTTGAGTATTGATGAGTAAG+AGG | 0.374221 | 4_4:-68513226 | None:intergenic |
| GATTATGGTTGTTTGAATAT+GGG | 0.376348 | 4_4:-68513325 | None:intergenic |
| TGAGTAAGAGGAATTGGTTT+TGG | 0.465050 | 4_4:-68513214 | None:intergenic |
| TGAATATGGGAAATTTGTGT+AGG | 0.551742 | 4_4:-68513312 | None:intergenic |
| TGTAGGTGTGATAATGACAA+GGG | 0.641156 | 4_4:-68513295 | None:intergenic |
| GTGTAGGTGTGATAATGACA+AGG | 0.686955 | 4_4:-68513296 | None:intergenic |
| GTAGGTGTGATAATGACAAG+GGG | 0.735299 | 4_4:-68513294 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAACTATAGAGACATTGA+AGG | - | chr4_4:68513158-68513177 | None:intergenic | 25.0% |
| TGAAGGAAATTATCTTCATG+AGG | - | chr4_4:68513141-68513160 | None:intergenic | 30.0% | |
| TATTGATGAGTAAGAGGAAT+TGG | - | chr4_4:68513223-68513242 | None:intergenic | 30.0% | |
| !! | TTTGAGTATTGATGAGTAAG+AGG | - | chr4_4:68513229-68513248 | None:intergenic | 30.0% |
| TGAATATGGGAAATTTGTGT+AGG | - | chr4_4:68513315-68513334 | None:intergenic | 30.0% | |
| !! | CTCATTTTTCACAGTTGTAC+TGG | + | chr4_4:68513071-68513090 | Msa0698270:CDS | 35.0% |
| GAAATTATCTTCATGAGGAC+TGG | - | chr4_4:68513136-68513155 | None:intergenic | 35.0% | |
| !! | TGAGTAAGAGGAATTGGTTT+TGG | - | chr4_4:68513217-68513236 | None:intergenic | 35.0% |
| ! | TGTAGGTGTGATAATGACAA+GGG | - | chr4_4:68513298-68513317 | None:intergenic | 35.0% |
| ACTGTGAAAAATGAGCGTAG+AGG | - | chr4_4:68513066-68513085 | None:intergenic | 40.0% | |
| !! | GTGATAATGACAAGGGGTTT+TGG | - | chr4_4:68513291-68513310 | None:intergenic | 40.0% |
| ! | GTAGGTGTGATAATGACAAG+GGG | - | chr4_4:68513297-68513316 | None:intergenic | 40.0% |
| ! | GTGTAGGTGTGATAATGACA+AGG | - | chr4_4:68513299-68513318 | None:intergenic | 40.0% |
| !!! | AAGGGGTTTTGGTGTTTCGT+TGG | - | chr4_4:68513280-68513299 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 68513057 | 68513345 | 68513057 | ID=Msa0698270;Name=Msa0698270 |
| chr4_4 | mRNA | 68513057 | 68513345 | 68513057 | ID=Msa0698270-mRNA-1;Parent=Msa0698270;Name=Msa0698270-mRNA-1;_AED=0.49;_eAED=0.84;_QI=0|0|0|1|0|0|2|0|83 |
| chr4_4 | exon | 68513057 | 68513135 | 68513057 | ID=Msa0698270-mRNA-1:exon:20408;Parent=Msa0698270-mRNA-1 |
| chr4_4 | exon | 68513173 | 68513345 | 68513173 | ID=Msa0698270-mRNA-1:exon:20409;Parent=Msa0698270-mRNA-1 |
| chr4_4 | CDS | 68513057 | 68513135 | 68513057 | ID=Msa0698270-mRNA-1:cds;Parent=Msa0698270-mRNA-1 |
| chr4_4 | CDS | 68513173 | 68513345 | 68513173 | ID=Msa0698270-mRNA-1:cds;Parent=Msa0698270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
