Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0698630 | KEH31381.1 | 92.308 | 78 | 4 | 1 | 16 | 93 | 15 | 90 | 3.37e-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0698630 | A0A072UPW7 | 92.308 | 78 | 4 | 1 | 16 | 93 | 15 | 90 | 1.61e-40 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0615670 | Msa0698630 | 0.895778 | 6.934414e-76 | -8.615850e-47 |
| Msa0641340 | Msa0698630 | 0.803908 | 2.784553e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0698630 | MtrunA17_Chr4g0052071 | 93.333 | 75 | 3 | 1 | 19 | 93 | 1 | 73 | 4.66e-43 | 134 |
| Msa0698630 | MtrunA17_Chr4g0052051 | 90.667 | 75 | 6 | 1 | 19 | 93 | 1 | 74 | 7.21e-42 | 131 |
| Msa0698630 | MtrunA17_Chr5g0443071 | 46.739 | 92 | 37 | 4 | 5 | 93 | 4 | 86 | 3.44e-18 | 72.0 |
| Msa0698630 | MtrunA17_Chr1g0152631 | 47.826 | 69 | 32 | 1 | 25 | 93 | 5 | 69 | 7.84e-14 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTTTGCCTGTTTCGTTCA+TGG | 0.301987 | 4_4:-68970778 | Msa0698630:CDS |
| TAAGGTTGTCTCTGCCTTTG+AGG | 0.398295 | 4_4:-68970742 | Msa0698630:CDS |
| AAAACGGCATGTCAATTGTT+GGG | 0.435149 | 4_4:-68970896 | Msa0698630:CDS |
| ATATCTTCCATGAACGAAAC+AGG | 0.438842 | 4_4:+68970771 | None:intergenic |
| CCATGCTCTTTGTTGCTACA+AGG | 0.439882 | 4_4:-68970944 | Msa0698630:CDS |
| CCTTGTAGCAACAAAGAGCA+TGG | 0.508487 | 4_4:+68970944 | None:intergenic |
| CTTGTAGCAACAAAGAGCAT+GGG | 0.548774 | 4_4:+68970945 | None:intergenic |
| AACAAGAACAACAGCCTCAA+AGG | 0.548810 | 4_4:+68970728 | None:intergenic |
| CATCATCATCAGTATTCAAG+TGG | 0.550675 | 4_4:-68970809 | Msa0698630:CDS |
| AGAAAAGATCGTTGTTGAAG+AGG | 0.565815 | 4_4:-68970872 | Msa0698630:CDS |
| GTTGTTGAAGAGGCTGAAGA+AGG | 0.571156 | 4_4:-68970862 | Msa0698630:CDS |
| AACATAGATAAAAGAAGCAG+AGG | 0.576392 | 4_4:+68970700 | None:intergenic |
| ACATAGATAAAAGAAGCAGA+GGG | 0.638009 | 4_4:+68970701 | None:intergenic |
| GGCTGAAGAAGGAAACATTG+AGG | 0.704429 | 4_4:-68970851 | Msa0698630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGCAAAGTTGATTAAAAA+CGG | - | chr4_4:68970713-68970732 | Msa0698630:CDS | 20.0% |
| !!! | CATGGAAGATATTGTTTTTA+AGG | - | chr4_4:68970865-68970884 | Msa0698630:CDS | 25.0% |
| ACATAGATAAAAGAAGCAGA+GGG | + | chr4_4:68970927-68970946 | None:intergenic | 30.0% | |
| AACATAGATAAAAGAAGCAG+AGG | + | chr4_4:68970928-68970947 | None:intergenic | 30.0% | |
| ! | TGCTTCTTTTATCTATGTTG+TGG | - | chr4_4:68970929-68970948 | Msa0698630:CDS | 30.0% |
| AAAAACGGCATGTCAATTGT+TGG | - | chr4_4:68970728-68970747 | Msa0698630:CDS | 35.0% | |
| AAAACGGCATGTCAATTGTT+GGG | - | chr4_4:68970729-68970748 | Msa0698630:CDS | 35.0% | |
| AGAAAAGATCGTTGTTGAAG+AGG | - | chr4_4:68970753-68970772 | Msa0698630:CDS | 35.0% | |
| ! | CATTGAGGTTGAAGTTTTTG+TGG | - | chr4_4:68970789-68970808 | Msa0698630:CDS | 35.0% |
| CATCATCATCAGTATTCAAG+TGG | - | chr4_4:68970816-68970835 | Msa0698630:CDS | 35.0% | |
| ATATCTTCCATGAACGAAAC+AGG | + | chr4_4:68970857-68970876 | None:intergenic | 35.0% | |
| TACTTTGCCTGTTTCGTTCA+TGG | - | chr4_4:68970847-68970866 | Msa0698630:CDS | 40.0% | |
| AACAAGAACAACAGCCTCAA+AGG | + | chr4_4:68970900-68970919 | None:intergenic | 40.0% | |
| GTTGTTGAAGAGGCTGAAGA+AGG | - | chr4_4:68970763-68970782 | Msa0698630:CDS | 45.0% | |
| GGCTGAAGAAGGAAACATTG+AGG | - | chr4_4:68970774-68970793 | Msa0698630:CDS | 45.0% | |
| TAAGGTTGTCTCTGCCTTTG+AGG | - | chr4_4:68970883-68970902 | Msa0698630:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 68970683 | 68970964 | 68970683 | ID=Msa0698630;Name=Msa0698630 |
| chr4_4 | mRNA | 68970683 | 68970964 | 68970683 | ID=Msa0698630-mRNA-1;Parent=Msa0698630;Name=Msa0698630-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|93 |
| chr4_4 | exon | 68970683 | 68970964 | 68970683 | ID=Msa0698630-mRNA-1:exon:20587;Parent=Msa0698630-mRNA-1 |
| chr4_4 | CDS | 68970683 | 68970964 | 68970683 | ID=Msa0698630-mRNA-1:cds;Parent=Msa0698630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
