Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699740 | XP_013467927.1 | 97.241 | 145 | 3 | 1 | 1 | 145 | 1 | 144 | 5.23e-96 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699740 | sp|P49215|RS17_SOLLC | 87.023 | 131 | 15 | 1 | 1 | 129 | 1 | 131 | 2.24e-77 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699740 | I3SPL5 | 97.241 | 145 | 3 | 1 | 1 | 145 | 1 | 144 | 2.50e-96 | 283 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0616410 | Msa0699740 | 0.934360 | 4.559025e-96 | -8.615850e-47 |
| Msa0699740 | Msa1211770 | 0.827558 | 1.464363e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699740 | MtrunA17_Chr1g0178041 | 97.241 | 145 | 3 | 1 | 1 | 145 | 1 | 144 | 4.81e-100 | 283 |
| Msa0699740 | MtrunA17_Chr8g0375961 | 96.552 | 145 | 4 | 1 | 1 | 145 | 1 | 144 | 3.32e-98 | 278 |
| Msa0699740 | MtrunA17_Chr8g0375841 | 96.552 | 145 | 4 | 1 | 1 | 145 | 1 | 144 | 3.32e-98 | 278 |
| Msa0699740 | MtrunA17_Chr8g0375941 | 88.966 | 145 | 11 | 1 | 1 | 145 | 1 | 140 | 1.18e-86 | 249 |
| Msa0699740 | MtrunA17_Chr8g0375831 | 91.270 | 126 | 11 | 0 | 1 | 126 | 1 | 126 | 9.38e-79 | 228 |
| Msa0699740 | MtrunA17_Chr8g0375931 | 89.256 | 121 | 8 | 1 | 24 | 144 | 1 | 116 | 1.11e-71 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0699740 | AT5G04800.2 | 77.931 | 145 | 28 | 1 | 1 | 145 | 1 | 141 | 2.77e-76 | 223 |
| Msa0699740 | AT5G04800.4 | 77.931 | 145 | 28 | 1 | 1 | 145 | 1 | 141 | 2.77e-76 | 223 |
| Msa0699740 | AT5G04800.3 | 77.931 | 145 | 28 | 1 | 1 | 145 | 1 | 141 | 2.77e-76 | 223 |
| Msa0699740 | AT5G04800.1 | 77.931 | 145 | 28 | 1 | 1 | 145 | 1 | 141 | 2.77e-76 | 223 |
| Msa0699740 | AT2G04390.1 | 78.231 | 147 | 24 | 3 | 1 | 145 | 1 | 141 | 1.22e-74 | 219 |
| Msa0699740 | AT2G05220.2 | 75.168 | 149 | 24 | 2 | 1 | 145 | 1 | 140 | 1.23e-74 | 219 |
| Msa0699740 | AT2G05220.1 | 75.168 | 149 | 24 | 2 | 1 | 145 | 1 | 140 | 1.23e-74 | 219 |
| Msa0699740 | AT3G10610.1 | 80.576 | 139 | 20 | 2 | 1 | 135 | 1 | 136 | 1.33e-74 | 219 |
Find 44 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGATTTCTTCACGGTTT+TGG | 0.256241 | 4_4:+70508171 | None:intergenic |
| CGAGATTCCCGGTGTTGTTC+AGG | 0.275016 | 4_4:-70507852 | Msa0699740:CDS |
| CGTCTTCGCAACAAGATTGC+TGG | 0.339095 | 4_4:-70508055 | Msa0699740:CDS |
| TTCCTGATGTTTCTGCTATT+AGG | 0.341443 | 4_4:-70507934 | Msa0699740:CDS |
| TTCTTCAAGGATCTTCTTGT+TGG | 0.343538 | 4_4:+70508099 | None:intergenic |
| TTGTTGCGAAGACGCTTTGA+TGG | 0.348895 | 4_4:+70508064 | None:intergenic |
| ATCCCATTCGGTCGTGGTGC+TGG | 0.359143 | 4_4:-70507806 | Msa0699740:CDS |
| CCGAATGGGATCTGCTGAAC+AGG | 0.361093 | 4_4:+70507818 | None:intergenic |
| GGTGCTGCTGGTGGTGGTGT+TGG | 0.368534 | 4_4:-70507785 | Msa0699740:CDS |
| GGGATCTGCTGAACAGGAAC+AGG | 0.372363 | 4_4:+70507824 | None:intergenic |
| GGTCGTGGTGCTGGTGCTGC+TGG | 0.373909 | 4_4:-70507797 | Msa0699740:CDS |
| CTGCTGGTGGTGGTGTTGGA+AGG | 0.382557 | 4_4:-70507781 | Msa0699740:CDS |
| CTTGGTATGTCCGAGATTCC+CGG | 0.385581 | 4_4:-70507863 | Msa0699740:CDS |
| CCTTCTGAATACGTTTCATA+AGG | 0.423532 | 4_4:+70508020 | None:intergenic |
| CTAGACATGCTTGCTGCTCT+TGG | 0.432500 | 4_4:-70507881 | Msa0699740:CDS |
| TTCAATGAAATTCCACGAAC+AGG | 0.443778 | 4_4:+70507995 | None:intergenic |
| GGTGCTGGTGCTGCTGGTGG+TGG | 0.444507 | 4_4:-70507791 | Msa0699740:CDS |
| CTGAACAACACCGGGAATCT+CGG | 0.461504 | 4_4:+70507853 | None:intergenic |
| GAATCAAAGCAACTTCTTCA+AGG | 0.471503 | 4_4:+70508086 | None:intergenic |
| CCTGTTCAGCAGATCCCATT+CGG | 0.473555 | 4_4:-70507818 | Msa0699740:CDS |
| CAAGAGCAGCAAGCATGTCT+AGG | 0.477650 | 4_4:+70507882 | None:intergenic |
| TGATCATATTGAGGTTGATA+AGG | 0.481558 | 4_4:-70507909 | Msa0699740:CDS |
| CAAGGATCTTCTTGTTGGTG+TGG | 0.483634 | 4_4:+70508104 | None:intergenic |
| TTGACGAGATGATTTCTTCA+CGG | 0.484691 | 4_4:+70508165 | None:intergenic |
| CACCAGCACCACGACCGAAT+GGG | 0.505188 | 4_4:+70507804 | None:intergenic |
| GTCCTAATAGCAGAAACATC+AGG | 0.505221 | 4_4:+70507932 | None:intergenic |
| CTGGTGGTGGTGTTGGAAGG+AGG | 0.512573 | 4_4:-70507778 | Msa0699740:CDS |
| GGAGGAGCGTGAGCGTCGTA+TGG | 0.521163 | 4_4:-70507963 | Msa0699740:CDS |
| TTTGGTTCGGACTCGACCCA+TGG | 0.522820 | 4_4:+70508189 | None:intergenic |
| ATTCAGAAGGGACCTGTTCG+TGG | 0.526647 | 4_4:-70508007 | Msa0699740:CDS |
| CGTGGTGCTGGTGCTGCTGG+TGG | 0.530900 | 4_4:-70507794 | Msa0699740:CDS |
| CAGCAGATCCCATTCGGTCG+TGG | 0.540344 | 4_4:-70507812 | Msa0699740:CDS |
| GTGGAAATCGAGTGTCATCT+TGG | 0.542107 | 4_4:+70508123 | None:intergenic |
| TATTAGGACTGATCATATTG+AGG | 0.542204 | 4_4:-70507918 | Msa0699740:CDS |
| GCACCAGCACCACGACCGAA+TGG | 0.558262 | 4_4:+70507803 | None:intergenic |
| AAGAGCAGCAAGCATGTCTA+GGG | 0.560169 | 4_4:+70507883 | None:intergenic |
| TTCATTGAAGCTGCAAGAGG+AGG | 0.567846 | 4_4:-70507981 | Msa0699740:CDS |
| CCTTATGAAACGTATTCAGA+AGG | 0.588661 | 4_4:-70508020 | Msa0699740:CDS |
| AGGGTCAACCTGAACAACAC+CGG | 0.593915 | 4_4:+70507844 | None:intergenic |
| GGGTCAACCTGAACAACACC+GGG | 0.608103 | 4_4:+70507845 | None:intergenic |
| AATACGTTTCATAAGGTGAG+TGG | 0.609703 | 4_4:+70508027 | None:intergenic |
| AATTTCATTGAAGCTGCAAG+AGG | 0.634258 | 4_4:-70507984 | Msa0699740:CDS |
| CTTATGAAACGTATTCAGAA+GGG | 0.635445 | 4_4:-70508019 | Msa0699740:CDS |
| GGATCTGCTGAACAGGAACA+GGG | 0.673645 | 4_4:+70507825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTATGAAACGTATTCAGAA+GGG | - | chr4_4:70507940-70507959 | Msa0699740:CDS | 30.0% | |
| TATTAGGACTGATCATATTG+AGG | - | chr4_4:70508041-70508060 | Msa0699740:CDS | 30.0% | |
| !! | TGATCATATTGAGGTTGATA+AGG | - | chr4_4:70508050-70508069 | Msa0699740:CDS | 30.0% |
| !!! | ATTTCTTCACGGTTTTGGTT+CGG | + | chr4_4:70507786-70507805 | None:intergenic | 35.0% |
| ! | AGATGATTTCTTCACGGTTT+TGG | + | chr4_4:70507791-70507810 | None:intergenic | 35.0% |
| TTGACGAGATGATTTCTTCA+CGG | + | chr4_4:70507797-70507816 | None:intergenic | 35.0% | |
| TTCTTCAAGGATCTTCTTGT+TGG | + | chr4_4:70507863-70507882 | None:intergenic | 35.0% | |
| GAATCAAAGCAACTTCTTCA+AGG | + | chr4_4:70507876-70507895 | None:intergenic | 35.0% | |
| AATACGTTTCATAAGGTGAG+TGG | + | chr4_4:70507935-70507954 | None:intergenic | 35.0% | |
| CCTTCTGAATACGTTTCATA+AGG | + | chr4_4:70507942-70507961 | None:intergenic | 35.0% | |
| CCTTATGAAACGTATTCAGA+AGG | - | chr4_4:70507939-70507958 | Msa0699740:CDS | 35.0% | |
| TTCAATGAAATTCCACGAAC+AGG | + | chr4_4:70507967-70507986 | None:intergenic | 35.0% | |
| AATTTCATTGAAGCTGCAAG+AGG | - | chr4_4:70507975-70507994 | Msa0699740:CDS | 35.0% | |
| ! | TTCCTGATGTTTCTGCTATT+AGG | - | chr4_4:70508025-70508044 | Msa0699740:CDS | 35.0% |
| GTCCTAATAGCAGAAACATC+AGG | + | chr4_4:70508030-70508049 | None:intergenic | 40.0% | |
| GTGGAAATCGAGTGTCATCT+TGG | + | chr4_4:70507839-70507858 | None:intergenic | 45.0% | |
| CAAGGATCTTCTTGTTGGTG+TGG | + | chr4_4:70507858-70507877 | None:intergenic | 45.0% | |
| TTGTTGCGAAGACGCTTTGA+TGG | + | chr4_4:70507898-70507917 | None:intergenic | 45.0% | |
| TTCATTGAAGCTGCAAGAGG+AGG | - | chr4_4:70507978-70507997 | Msa0699740:CDS | 45.0% | |
| AAGAGCAGCAAGCATGTCTA+GGG | + | chr4_4:70508079-70508098 | None:intergenic | 45.0% | |
| CGTCTTCGCAACAAGATTGC+TGG | - | chr4_4:70507904-70507923 | Msa0699740:CDS | 50.0% | |
| ATTCAGAAGGGACCTGTTCG+TGG | - | chr4_4:70507952-70507971 | Msa0699740:CDS | 50.0% | |
| CAAGAGCAGCAAGCATGTCT+AGG | + | chr4_4:70508080-70508099 | None:intergenic | 50.0% | |
| ! | CTAGACATGCTTGCTGCTCT+TGG | - | chr4_4:70508078-70508097 | Msa0699740:CDS | 50.0% |
| CTTGGTATGTCCGAGATTCC+CGG | - | chr4_4:70508096-70508115 | Msa0699740:CDS | 50.0% | |
| CTGAACAACACCGGGAATCT+CGG | + | chr4_4:70508109-70508128 | None:intergenic | 50.0% | |
| AGGGTCAACCTGAACAACAC+CGG | + | chr4_4:70508118-70508137 | None:intergenic | 50.0% | |
| GGATCTGCTGAACAGGAACA+GGG | + | chr4_4:70508137-70508156 | None:intergenic | 50.0% | |
| CCTGTTCAGCAGATCCCATT+CGG | - | chr4_4:70508141-70508160 | Msa0699740:CDS | 50.0% | |
| !! | CGAGATTCCCGGTGTTGTTC+AGG | - | chr4_4:70508107-70508126 | Msa0699740:CDS | 55.0% |
| GGGTCAACCTGAACAACACC+GGG | + | chr4_4:70508117-70508136 | None:intergenic | 55.0% | |
| GGGATCTGCTGAACAGGAAC+AGG | + | chr4_4:70508138-70508157 | None:intergenic | 55.0% | |
| CCGAATGGGATCTGCTGAAC+AGG | + | chr4_4:70508144-70508163 | None:intergenic | 55.0% | |
| CAGCAGATCCCATTCGGTCG+TGG | - | chr4_4:70508147-70508166 | Msa0699740:CDS | 60.0% | |
| ATCCCATTCGGTCGTGGTGC+TGG | - | chr4_4:70508153-70508172 | Msa0699740:CDS | 60.0% | |
| CACCAGCACCACGACCGAAT+GGG | + | chr4_4:70508158-70508177 | None:intergenic | 60.0% | |
| !! | CTGCTGGTGGTGGTGTTGGA+AGG | - | chr4_4:70508178-70508197 | Msa0699740:CDS | 60.0% |
| !! | CTGGTGGTGGTGTTGGAAGG+AGG | - | chr4_4:70508181-70508200 | Msa0699740:CDS | 60.0% |
| ! | GGAGGAGCGTGAGCGTCGTA+TGG | - | chr4_4:70507996-70508015 | Msa0699740:CDS | 65.0% |
| GCACCAGCACCACGACCGAA+TGG | + | chr4_4:70508159-70508178 | None:intergenic | 65.0% | |
| !! | GGTGCTGCTGGTGGTGGTGT+TGG | - | chr4_4:70508174-70508193 | Msa0699740:CDS | 65.0% |
| !! | GGTCGTGGTGCTGGTGCTGC+TGG | - | chr4_4:70508162-70508181 | Msa0699740:CDS | 70.0% |
| !! | CGTGGTGCTGGTGCTGCTGG+TGG | - | chr4_4:70508165-70508184 | Msa0699740:CDS | 70.0% |
| !! | GGTGCTGGTGCTGCTGGTGG+TGG | - | chr4_4:70508168-70508187 | Msa0699740:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 70507772 | 70508209 | 70507772 | ID=Msa0699740;Name=Msa0699740 |
| chr4_4 | mRNA | 70507772 | 70508209 | 70507772 | ID=Msa0699740-mRNA-1;Parent=Msa0699740;Name=Msa0699740-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|145 |
| chr4_4 | exon | 70507772 | 70508209 | 70507772 | ID=Msa0699740-mRNA-1:exon:21361;Parent=Msa0699740-mRNA-1 |
| chr4_4 | CDS | 70507772 | 70508209 | 70507772 | ID=Msa0699740-mRNA-1:cds;Parent=Msa0699740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
