AlfalfaGEDB | Msa1211770

M. sativa cultivar zhongmu-4 / Msa1211770

KEGG: K02997
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005911 GO:0006417 GO:0006450 GO:0008150 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0015935 GO:0016020 GO:0019222 GO:0019843 GO:0022626 GO:0022627 GO:0030054 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0032991 GO:0034248 GO:0034250 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045727 GO:0045903 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112
PFAM: PF00163.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211770	sp\|Q9FLF0\|RS92_ARATH	90.909	187	17	0	1	187	1	187	2.10e-123	349

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211770	B7FMD4	100.000	197	0	0	1	197	1	197	2.47e-142	404

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0032810	Msa1211770	0.842902	1.947404e-58	-8.615850e-47
Msa0118710	Msa1211770	0.810874	9.279405e-51	-8.615850e-47
Msa0142110	Msa1211770	0.825284	5.096833e-54	-8.615850e-47
Msa0167480	Msa1211770	0.815755	7.856390e-52	-8.615850e-47
Msa0188690	Msa1211770	0.804875	1.751110e-49	-8.615850e-47
Msa0219120	Msa1211770	0.843944	1.025679e-58	-8.615850e-47
Msa0221030	Msa1211770	0.800537	1.375609e-48	-8.615850e-47
Msa0231100	Msa1211770	0.809525	1.812724e-50	-8.615850e-47
Msa0258850	Msa1211770	0.831932	1.261207e-55	-8.615850e-47
Msa0262480	Msa1211770	0.832917	7.190804e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211770	MtrunA17_Chr3g0077371	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1211770	MtrunA17_Chr3g0077391	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1211770	MtrunA17_Chr4g0009481	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1211770	MtrunA17_Chr7g0241891	98.985	197	2	1	197	1	197	2.61e-144	399
Msa1211770	MtrunA17_Chr1g0182531	98.477	197	3	1	197	28	224	3.76e-143	397
Msa1211770	MtrunA17_Chr4g0060551	98.261	115	2	1	115	1	115	5.75e-79	231
Msa1211770	MtrunA17_Chr4g0009501	100.000	60	0	138	197	13	72	4.70e-38	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1211770	AT5G39850.1	90.909	187	17	0	1	187	1	187	2.13e-124	349
Msa1211770	AT5G15200.1	84.343	198	30	1	1	197	1	198	9.63e-118	332
Msa1211770	AT5G15200.2	89.051	137	15	0	1	137	1	137	4.16e-86	251

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Find 79 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCGTTCTAGCATTGTTA+CGG	0.481953	8_1:+71504490	None:intergenic
AAAGTTTCGGTTGGATACAT+GGG	0.591867	8_1:+71502532	None:intergenic
AACCTAGTAGGCAACACCGG+CGG	0.720584	8_1:-71504709	Msa1211770:five_prime_UTR
AACTATGCTTCTTATGGTGT+AGG	0.569156	8_1:-71502504	Msa1211770:three_prime_UTR
AACTTGTATAAAGATCAAGC+AGG	0.637230	8_1:+71502432	None:intergenic
AAGAACCCTAGCATGGTGAA+TGG	0.539163	8_1:+71504284	None:intergenic
AAGAAGGCTTCCGGTGGTGA+TGG	0.531981	8_1:-71502858	Msa1211770:CDS
AATCCTCGTCGGATCTTCGA+GGG	0.591722	8_1:-71504450	Msa1211770:CDS
ACAAAGATCATCAAATGTAT+TGG	0.418707	8_1:+71502634	None:intergenic
ACATCCCATCCTTCATGGTT+AGG	0.353382	8_1:-71502977	Msa1211770:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTGCGAAAGCAATTATAAT+AGG	+	chr8_1:71503010-71503029	None:intergenic	30.0%
AAAGAGTTACAAGCAGTTAT+GGG	+	chr8_1:71503711-71503730	None:intergenic	30.0%
AAGTAGAGATCATCAAGTAA+TGG	-	chr8_1:71503803-71503822	Msa1211770:intron	30.0%
ACACTAACAAGTATAGTGTT+AGG	+	chr8_1:71503878-71503897	None:intergenic	30.0%
TCTATTTACTTACCTCTGAA+TGG	-	chr8_1:71503928-71503947	Msa1211770:intron	30.0%
CATTTATTGTTTCATAGGGT+TGG	-	chr8_1:71504062-71504081	Msa1211770:intron	30.0%
ATTTCCTCACACAATAAACA+AGG	+	chr8_1:71504366-71504385	None:intergenic	30.0%
TACACACAACATCAAGTAAA+AGG	+	chr8_1:71504478-71504497	None:intergenic	30.0%
AACTTGTATAAAGATCAAGC+AGG	+	chr8_1:71504647-71504666	None:intergenic	30.0%
TTTGTGATTAAAATGTCAGG+TGG	+	chr8_1:71504701-71504720	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	71502351	71504747	71502351	ID=Msa1211770;Name=Msa1211770
chr8_1	mRNA	71502351	71504747	71502351	ID=Msa1211770-mRNA-1;Parent=Msa1211770;Name=Msa1211770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=74\|1\|1\|1\|1\|1\|2\|485\|197
chr8_1	exon	71502351	71503019	71502351	ID=Msa1211770-mRNA-1:exon:24089;Parent=Msa1211770-mRNA-1
chr8_1	exon	71504264	71504747	71504264	ID=Msa1211770-mRNA-1:exon:24088;Parent=Msa1211770-mRNA-1
chr8_1	five_prime_UTR	71504674	71504747	71504674	ID=Msa1211770-mRNA-1:five_prime_utr;Parent=Msa1211770-mRNA-1
chr8_1	CDS	71504264	71504673	71504264	ID=Msa1211770-mRNA-1:cds;Parent=Msa1211770-mRNA-1
chr8_1	CDS	71502836	71503019	71502836	ID=Msa1211770-mRNA-1:cds;Parent=Msa1211770-mRNA-1
chr8_1	three_prime_UTR	71502351	71502835	71502351	ID=Msa1211770-mRNA-1:three_prime_utr;Parent=Msa1211770-mRNA-1

Gene Sequence

>Msa1211770

ATGGTGCACGTTGCGTTTTATCGAAACTACGGCAAAACATTCAAGAAGCCTCGTCGTCCATATGAGAAGGAGCGTTTGGATGCTGAGTTGAAGCTCGTCGGAGAGTATGGTCTTCGCTGCAAGCGCGAGCTTTGGAGGGTTCAATACGCTCTTAGCCGTATCCGTAACAATGCTAGAACGCTTTTGACACTGGATGAGAAGAATCCTCGTCGGATCTTCGAGGGTGAAGCTCTTTTGAGGAGGATGTTTAAGCATGGCCTTCTAGATGAGACACAGAACAAGCTCGATTATGTGTTGGCTCTTACTGTTGAGAATTTTCTCGAGCGTCGTCTTCAGACACTCGTGTTCAAATCTGGCATGGCTAAGTCCATTCACCATGCTAGGGTTCTTATCAGGCAGAGGCACATCAGGGTTGGGAGGCAGGTGGTGAACATCCCATCCTTCATGGTTAGGGTTGATTCACAGAAGCACATTGACTTTTCACTCACAAGTCCTTTCGGTGGTGGTCTTCCTGGTCGTGTGAAGCGAAAGAACCTTAAGGCTGCAGCCAAGAAGGCTTCCGGTGGTGATGGTGATGAGGAGGATGAAGATTAA

Protein sequence

>Msa1211770

MVHVAFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYALSRIRNNARTLLTLDEKNPRRIFEGEALLRRMFKHGLLDETQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQVVNIPSFMVRVDSQKHIDFSLTSPFGGGLPGRVKRKNLKAAAKKASGGDGDEEDED*