AlfalfaGEDB | Msa0705160

M. sativa cultivar zhongmu-4 / Msa0705160

KEGG: K09422
GO: GO:0000228 GO:0000723 GO:0000781 GO:0000784 GO:0000785 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003691 GO:0003697 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008134 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010468 GO:0010556 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031627 GO:0031974 GO:0031981 GO:0032200 GO:0033613 GO:0033993 GO:0034641 GO:0042162 GO:0042221 GO:0042493 GO:0042592 GO:0042802 GO:0042803 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046677 GO:0046686 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0070491 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097305 GO:0098687 GO:0098847 GO:0140110 GO:1901360 GO:1901363 GO:1901700 GO:1903506 GO:1990841 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705160	XP_024629648.1	88.933	253	15	1	1	240	1	253	1.03e-158	454

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705160	sp\|Q8VWK4\|TRB1_ARATH	46.063	254	106	8	1	237	1	240	2.65e-64	207

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705160	G7LCB1	88.933	253	15	1	1	240	1	253	6.04e-157	455

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TFs/TRs:

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Gene ID	Type	Classification
Msa0705160	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0659170	Msa0705160	0.969299	6.411184e-130	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705160	MtrunA17_Chr2g0293381	61.694	248	73	2	1	235	1	239	1.53e-100	296
Msa0705160	MtrunA17_Chr8g0384671	62.602	246	72	4	1	235	1	237	3.39e-99	293
Msa0705160	MtrunA17_Chr8g0383821	92.857	112	8	0	1	112	1	112	1.84e-73	221
Msa0705160	MtrunA17_Chr4g0054131	43.131	313	140	7	1	286	1	302	1.47e-48	163
Msa0705160	MtrunA17_Chr5g0401061	44.000	300	147	4	1	289	1	290	4.18e-48	162
Msa0705160	MtrunA17_Chr8g0383841	89.706	68	7	0	173	240	1	68	5.45e-35	122
Msa0705160	MtrunA17_Chr1g0195251	51.969	127	49	3	112	238	295	409	1.03e-31	122
Msa0705160	MtrunA17_Chr2g0329921	33.482	224	81	6	1	174	1	206	3.05e-27	107
Msa0705160	MtrunA17_Chr8g0342871	31.250	304	156	9	1	283	1	272	4.98e-27	106
Msa0705160	MtrunA17_Chr1g0166681	67.188	64	21	0	1	64	1	64	2.91e-25	99.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705160	AT1G49950.3	46.063	254	106	8	1	237	1	240	2.69e-65	207
Msa0705160	AT1G49950.1	46.063	254	106	8	1	237	1	240	2.69e-65	207
Msa0705160	AT1G49950.2	46.063	254	106	8	1	237	1	240	2.69e-65	207
Msa0705160	AT5G67580.2	41.722	302	149	6	1	286	1	291	1.67e-42	148
Msa0705160	AT5G67580.1	41.722	302	149	6	1	286	1	291	1.67e-42	148
Msa0705160	AT3G49850.1	36.239	218	126	2	1	206	1	217	2.17e-40	142
Msa0705160	AT3G49850.2	36.239	218	126	2	1	206	1	217	2.17e-40	142
Msa0705160	AT1G72740.2	34.673	199	118	3	1	188	1	198	2.06e-29	113
Msa0705160	AT1G72740.1	36.313	179	103	2	1	168	1	179	7.33e-28	109
Msa0705160	AT1G17520.1	36.316	190	99	3	1	170	1	188	6.81e-25	101

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Find 73 sgRNAs with CRISPR-Local

Find 378 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCATACCTGGCTGGC+TGG	0.506502	4_4:+77719413	None:intergenic
AAACCGACCCATGTTATTAT+TGG	0.212298	4_4:-77714739	Msa0705160:CDS
AAAGCTGGAGTTGTGAAGCA+TGG	0.620365	4_4:-77718550	Msa0705160:CDS
AAAGTCCATGTCTTTACAAG+AGG	0.539777	4_4:-77714601	Msa0705160:CDS
AAATAAGCTGTCAGCAAAAC+TGG	0.353881	4_4:-77715751	Msa0705160:CDS
AACTATTGCTTCTTTCATTA+AGG	0.194532	4_4:-77717195	Msa0705160:intron
AAGAAAGGCTGAGATAGACT+TGG	0.566957	4_4:-77714637	Msa0705160:CDS
AAGAATGATTTCTCTCCAAT+TGG	0.402800	4_4:-77719454	Msa0705160:five_prime_UTR
AAGCCTCTATTAAATCTGAC+AGG	0.463302	4_4:-77714704	Msa0705160:CDS
AAGGCAAATGGATCATCCTC+CGG	0.503120	4_4:-77718299	Msa0705160:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAATGATTTCTCTCCAAT+TGG	-	chr4_4:77714479-77714498	Msa0705160:CDS	30.0%
TGTGATGAAAAATTCATACC+TGG	+	chr4_4:77714531-77714550	None:intergenic	30.0%
CAAACTAAAACTTTCCTACA+TGG	+	chr4_4:77715157-77715176	None:intergenic	30.0%
ACTTGTGATTATATGTTTGG+AGG	-	chr4_4:77715245-77715264	Msa0705160:intron	30.0%
TAAATGGAGTAAGATACTCA+AGG	-	chr4_4:77715412-77715431	Msa0705160:intron	30.0%
ATCTCAATGTTTGGTTATGT+GGG	-	chr4_4:77715579-77715598	Msa0705160:intron	30.0%
TCAAGTTAAAACAGATATGC+TGG	-	chr4_4:77715777-77715796	Msa0705160:CDS	30.0%
AACAGATATGCTGGAAATTA+AGG	-	chr4_4:77715786-77715805	Msa0705160:intron	30.0%
ACAGATATGCTGGAAATTAA+GGG	-	chr4_4:77715787-77715806	Msa0705160:intron	30.0%
TTCGACCAAATAAACCAATA+GGG	+	chr4_4:77716133-77716152	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	77714446	77719509	77714446	ID=Msa0705160;Name=Msa0705160
chr4_4	mRNA	77714446	77719509	77714446	ID=Msa0705160-mRNA-1;Parent=Msa0705160;Name=Msa0705160-mRNA-1;_AED=0.13;_eAED=0.13;_QI=114\|0.33\|0.28\|0.85\|0.83\|0.71\|7\|0\|289
chr4_4	exon	77714446	77714814	77714446	ID=Msa0705160-mRNA-1:exon:24750;Parent=Msa0705160-mRNA-1
chr4_4	exon	77715719	77715802	77715719	ID=Msa0705160-mRNA-1:exon:24749;Parent=Msa0705160-mRNA-1
chr4_4	exon	77717196	77717280	77717196	ID=Msa0705160-mRNA-1:exon:24748;Parent=Msa0705160-mRNA-1
chr4_4	exon	77717360	77717370	77717360	ID=Msa0705160-mRNA-1:exon:24747;Parent=Msa0705160-mRNA-1
chr4_4	exon	77718175	77718345	77718175	ID=Msa0705160-mRNA-1:exon:24746;Parent=Msa0705160-mRNA-1
chr4_4	exon	77718471	77718648	77718471	ID=Msa0705160-mRNA-1:exon:24745;Parent=Msa0705160-mRNA-1
chr4_4	exon	77719424	77719509	77719424	ID=Msa0705160-mRNA-1:exon:24744;Parent=Msa0705160-mRNA-1
chr4_4	five_prime_UTR	77719424	77719509	77719424	ID=Msa0705160-mRNA-1:five_prime_utr;Parent=Msa0705160-mRNA-1
chr4_4	five_prime_UTR	77718621	77718648	77718621	ID=Msa0705160-mRNA-1:five_prime_utr;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77718471	77718620	77718471	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77718175	77718345	77718175	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77717360	77717370	77717360	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77717196	77717280	77717196	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77715719	77715802	77715719	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1
chr4_4	CDS	77714446	77714814	77714446	ID=Msa0705160-mRNA-1:cds;Parent=Msa0705160-mRNA-1

Gene Sequence

>Msa0705160

ATGGGTGCTACTAGACAGAAATGGTCATCAGAAGAGGAAGCGGCTCTCAAAGCTGGAGTTGTGAAGCATGGCGTAGGTAAATGGAGTAAGATACTCAAGGATCCTGAGTTTAACCATGTGTTGTATATCCGCTCAAATATCGATCTCAAGGATAAATGGAGAAATATGAGTCTGAAGGCAAATGGATCATCCTCCGGGGACAACTCTCAGCTAGCAATAAAAAGGGTGCGTCATCAGGCACCAGAACAGCGTGACGACTCCGTGGCTGTAAATCTTGTTACTACTATTGATGATGAAATTCTTGATGTTCAGCCTCTTCAACATCGTGTAGAATTGGACAACCTTATAGTGGAAGCAATATCTAGCTTGAATGAGGTCGATGGTTCTAATAAAACAACTATTGCTTCTTTCATTAAGAACCAATACTGCGCACCGGCAGATTTCAAAAATAAGCTGTCAGCAAAACTGGTGGATCTTGTATCTAGTGGAAAACTGATCAAGGTGAAACATAGGTACATGATTACGCCTACCCAAGCATATTCAGATAGAGGAAGAAACCGACCCATGTTATTATTGGAAGGGAGGCAGAAAGCCTCTATTAAATCTGACAGGGGTGGGGGCAATATCCCTAAAAAGTATGACATAGATTATCAAAAAAGAAAGGCTGAGATAGACTTGGAATTGGAAAAGTTAAAGTCCATGTCTTTACAAGAGGTAATCGCATGTGCTGCTCAAGCAGTTGCTGAAGCAGAAGCCGCCATGGCAGAAGCTGAAGAGGCAACAAAGGAGGCAGAGGCTGCAGAAGCAGAAGCTGATGCAGCGGAAGCTATTGCAAAAGCTGCAGAAAAATCACTAAAATCACTGCGGTAA

Protein sequence

>Msa0705160

MGATRQKWSSEEEAALKAGVVKHGVGKWSKILKDPEFNHVLYIRSNIDLKDKWRNMSLKANGSSSGDNSQLAIKRVRHQAPEQRDDSVAVNLVTTIDDEILDVQPLQHRVELDNLIVEAISSLNEVDGSNKTTIASFIKNQYCAPADFKNKLSAKLVDLVSSGKLIKVKHRYMITPTQAYSDRGRNRPMLLLEGRQKASIKSDRGGGNIPKKYDIDYQKRKAEIDLELEKLKSMSLQEVIACAAQAVAEAEAAMAEAEEATKEAEAAEAEADAAEAIAKAAEKSLKSLR*