AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTCCATCCTTCGATAAT+TGG 0.175788 4_4:+78841697 Msa0705980:five_prime_UTR
GAGAGACTGAGAGAGTTTAT+TGG 0.213373 4_4:-78841524 None:intergenic
CTCGAAACTGAATGCGAAAT+TGG 0.231395 4_4:+78841734 Msa0705980:five_prime_UTR
GGTATCTTCCACAACATAAT+TGG 0.257131 4_4:-78843654 None:intergenic
TGAACTAACGACTTAATCTT+TGG 0.288762 4_4:-78843623 None:intergenic
CATCAGACTTTCTTCAACTT+TGG 0.295523 4_4:-78843728 None:intergenic
ACACATATTATATAATTAGA+TGG 0.310349 4_4:-78844795 None:intergenic
GGCAATGGAGGGAGAGTTAC+GGG 0.314560 4_4:-78841572 None:intergenic
AAGAGCAGTTGAATCTGAAT+TGG 0.315541 4_4:+78842805 Msa0705980:three_prime_UTR
TCAAGGACAGTTTAAATCTA+TGG 0.328807 4_4:-78843687 None:intergenic
ATGTCAAGCAGCTTGCTCTT+AGG 0.333923 4_4:-78843993 None:intergenic
TGTGGAAGATACCCGCTTAC+TGG 0.355520 4_4:+78843664 Msa0705980:three_prime_UTR
TCTTCTGAACATGGCGGAGG+TGG 0.356351 4_4:+78841770 Msa0705980:exon
CCTTGATTGATCGAACCATT+TGG 0.358921 4_4:+78843704 Msa0705980:three_prime_UTR
CACCTTAGTTTATCAATTTG+AGG 0.364536 4_4:-78842851 None:intergenic
TTCTTCAACTTTGGTCCAAA+TGG 0.374330 4_4:-78843719 None:intergenic
GGGCGGTAGTGGAAGAATAT+TGG 0.378469 4_4:-78841550 None:intergenic
AGAAGATCGGTGGAGTGTAT+TGG 0.380519 4_4:-78841491 None:intergenic
TCTTTATTTCGTGACTTTCA+AGG 0.387750 4_4:+78843762 Msa0705980:three_prime_UTR
TTCAGTTTCGAGGTTTGATT+CGG 0.392644 4_4:-78841723 None:intergenic
TTCAGTGTCATCTTCGTTGA+AGG 0.397009 4_4:-78841664 None:intergenic
ACGAAATAAAGATGGTTAGA+AGG 0.400421 4_4:-78843751 None:intergenic
CTTCTGAACATGGCGGAGGT+GGG 0.421413 4_4:+78841771 Msa0705980:exon
CGTGACTTTCAAGGATGTCC+TGG 0.424498 4_4:+78843771 Msa0705980:three_prime_UTR
TGAGAGAGTTTATTGGAAAG+TGG 0.427868 4_4:-78841517 None:intergenic
AGAGCAGTTGAATCTGAATT+GGG 0.432385 4_4:+78842806 Msa0705980:three_prime_UTR
CTCTTCCCCTCAACCTTCCC+TGG 0.437208 4_4:+78841856 Msa0705980:CDS
AGGCAATGGAGGGAGAGTTA+CGG 0.446903 4_4:-78841573 None:intergenic
CAATTTCGCATTCAGTTTCG+AGG 0.454261 4_4:-78841733 None:intergenic
TCTGAACATGGCGGAGGTGG+GGG 0.454703 4_4:+78841773 Msa0705980:exon
TCTTCCTTCTTCTTCGTCTC+CGG 0.458668 4_4:-78841809 None:intergenic
AACCTCAAATTGATAAACTA+AGG 0.465404 4_4:+78842849 Msa0705980:three_prime_UTR
TTCTGAACATGGCGGAGGTG+GGG 0.472424 4_4:+78841772 Msa0705980:exon
ATTAAGTCGTTAGTTCAAGA+AGG 0.483930 4_4:+78843629 Msa0705980:three_prime_UTR
GAAAGGTTGTTGCAAGGCCA+TGG 0.491501 4_4:+78844696 Msa0705980:three_prime_UTR
AAGAGAGAGAATAAGTACTT+AGG 0.497511 4_4:+78843828 Msa0705980:three_prime_UTR
TAAATCTATGGCCAGTAAGC+GGG 0.503326 4_4:-78843675 None:intergenic
GAAGAAGAAGGAAGAAGCCT+TGG 0.503538 4_4:+78841817 Msa0705980:CDS
CTCCGCCATGTTCAGAAGAT+CGG 0.507404 4_4:-78841766 None:intergenic
GAAGAAGGAAGAAGCCTTGG+AGG 0.507888 4_4:+78841820 Msa0705980:CDS
ACGACGGAGGGAGGCAATGG+AGG 0.514741 4_4:-78841584 None:intergenic
ATACATTGGCTGAAATTGAA+AGG 0.519500 4_4:+78844679 Msa0705980:three_prime_UTR
CCAAATGGTTCGATCAATCA+AGG 0.522065 4_4:-78843704 None:intergenic
ATCTTCCACGCCGTCAGTTG+CGG 0.522220 4_4:+78841610 Msa0705980:five_prime_UTR
ACGTTACCAGGGAAGGTTGA+GGG 0.525451 4_4:-78841862 None:intergenic
CGATCTTCTGAACATGGCGG+AGG 0.525577 4_4:+78841767 Msa0705980:exon
AGATGTTATTGGACATGTTG+TGG 0.526670 4_4:+78843935 Msa0705980:intron
TATTGGACATGTTGTGGAAA+GGG 0.530768 4_4:+78843941 Msa0705980:three_prime_UTR
TATTCTCTCTGTCCGAGTCC+AGG 0.532716 4_4:-78843789 None:intergenic
GATACGACGGAGGGAGGCAA+TGG 0.532828 4_4:-78841587 None:intergenic
TGAAAGTCACGAAATAAAGA+TGG 0.540847 4_4:-78843759 None:intergenic
CAATGGAGGGAGAGTTACGG+GGG 0.541480 4_4:-78841570 None:intergenic
CGACGGAGGGAGGCAATGGA+GGG 0.544268 4_4:-78841583 None:intergenic
CGTTGAAGGTGCTACGGCAG+CGG 0.546021 4_4:-78841650 None:intergenic
GCAATGGAGGGAGAGTTACG+GGG 0.546475 4_4:-78841571 None:intergenic
AGGGCCGGAGACGAAGAAGA+AGG 0.547231 4_4:+78841805 Msa0705980:CDS
TGGAAAGTGGAAGAGAAGAT+CGG 0.547485 4_4:-78841504 None:intergenic
TTAAATCTATGGCCAGTAAG+CGG 0.549553 4_4:-78843676 None:intergenic
CAAGCTGCTTGACATTACAT+TGG 0.561890 4_4:+78844001 Msa0705980:three_prime_UTR
AGAAGGTTCCAATTATGTTG+TGG 0.564107 4_4:+78843646 Msa0705980:three_prime_UTR
GGTTGCGACGTTACCAGGGA+AGG 0.568053 4_4:-78841869 None:intergenic
CACATATTATATAATTAGAT+GGG 0.568820 4_4:-78844794 None:intergenic
ATTCGGTTGCCAATTATCGA+AGG 0.569220 4_4:-78841706 None:intergenic
GAACGCTTTGAGTTCCTCCA+AGG 0.571802 4_4:-78841834 None:intergenic
CATCTTCGTTGAAGGTGCTA+CGG 0.573301 4_4:-78841656 None:intergenic
ATTATATAATTAGATGGGCA+TGG 0.580723 4_4:-78844789 None:intergenic
AACGTGATCACCGCAACTGA+CGG 0.581597 4_4:-78841620 None:intergenic
AAATATGAAAGTTTGTTGCA+AGG 0.582741 4_4:+78844722 Msa0705980:three_prime_UTR
GACGTTACCAGGGAAGGTTG+AGG 0.583197 4_4:-78841863 None:intergenic
GCTTTCCGATCTTCTGAACA+TGG 0.583486 4_4:+78841761 Msa0705980:exon
GGTTGCCAATTATCGAAGGA+TGG 0.588969 4_4:-78841702 None:intergenic
GAAATTGAAAGGTTGTTGCA+AGG 0.593760 4_4:+78844690 Msa0705980:three_prime_UTR
TTTCAAGGATGTCCTGGACT+CGG 0.595039 4_4:+78843777 Msa0705980:three_prime_UTR
AAAGTGGAAGAGAAGATCGG+TGG 0.599278 4_4:-78841501 None:intergenic
TTATTGGACATGTTGTGGAA+AGG 0.603102 4_4:+78843940 Msa0705980:three_prime_UTR
CGTTACCAGGGAAGGTTGAG+GGG 0.605910 4_4:-78841861 None:intergenic
GAAGATGATACGACGGAGGG+AGG 0.615514 4_4:-78841593 None:intergenic
GATCACCGCAACTGACGGCG+TGG 0.645915 4_4:-78841615 None:intergenic
GTGGAAGATGATACGACGGA+GGG 0.672586 4_4:-78841596 None:intergenic
TTCCGATCTTCTGAACATGG+CGG 0.698764 4_4:+78841764 Msa0705980:exon
CGGCGTGGAAGATGATACGA+CGG 0.703425 4_4:-78841600 None:intergenic
CGTGGAAGATGATACGACGG+AGG 0.705469 4_4:-78841597 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGAAAAAAATAGTATTT+GGG - chr4_4:78841906-78841925 None:intergenic 10.0%
!!! TTTATGTTATTAACATTTTA+AGG + chr4_4:78842705-78842724 Msa0705980:intron 10.0%
!!! TTTTCGAAATATATATTATT+TGG + chr4_4:78844042-78844061 Msa0705980:intron 10.0%
!! CAATTGAAAAAAATAGTATT+TGG - chr4_4:78841907-78841926 None:intergenic 15.0%
!!! TTAGTTTCTTTAATTTCAAT+TGG + chr4_4:78841968-78841987 Msa0705980:intron 15.0%
!!! TAGTTTCTTTAATTTCAATT+GGG + chr4_4:78841969-78841988 Msa0705980:intron 15.0%
!!! ATTTTTACAAATAAAAAGTC+AGG - chr4_4:78842551-78842570 None:intergenic 15.0%
!!! TTTTATTTGTAAAAATGACT+AGG + chr4_4:78842555-78842574 Msa0705980:intron 15.0%
!! AAATTTCATTCAAATTTAGT+CGG + chr4_4:78842874-78842893 Msa0705980:intron 15.0%
!!! ATAGATTTTGTTCAAATTAT+TGG + chr4_4:78842977-78842996 Msa0705980:intron 15.0%
!!! CAAAAATAAGATTGATTTTA+TGG - chr4_4:78843448-78843467 None:intergenic 15.0%
!! ATAAAAAACAAAGAAATCAA+TGG + chr4_4:78843969-78843988 Msa0705980:three_prime_UTR 15.0%
!! AAAGTGATAAAATAATATGT+TGG + chr4_4:78844202-78844221 Msa0705980:intron 15.0%
!! ATATGACATTGTTATATTTA+TGG + chr4_4:78844337-78844356 Msa0705980:intron 15.0%
!! TATGACATTGTTATATTTAT+GGG + chr4_4:78844338-78844357 Msa0705980:intron 15.0%
!! CACATATTATATAATTAGAT+GGG - chr4_4:78844797-78844816 None:intergenic 15.0%
!! ACACATATTATATAATTAGA+TGG - chr4_4:78844798-78844817 None:intergenic 15.0%
!! AATACACAAAAACAAATCAA+GGG - chr4_4:78842053-78842072 None:intergenic 20.0%
!! AAAAAAACATAACTTCAGAT+CGG - chr4_4:78842076-78842095 None:intergenic 20.0%
!! AGATTTATCTTTATTCTTGA+TGG + chr4_4:78842246-78842265 Msa0705980:intron 20.0%
!! AGTAATAAAAAACATTGATG+AGG - chr4_4:78842396-78842415 None:intergenic 20.0%
!! GTTATATTAGTTGAAGTTAT+TGG + chr4_4:78842524-78842543 Msa0705980:intron 20.0%
!! ACAAATAAGAATTAGAATAG+AGG - chr4_4:78843141-78843160 None:intergenic 20.0%
!!! ATTTCTATTGATTGCAAATT+TGG + chr4_4:78843362-78843381 Msa0705980:intron 20.0%
!!! TGATTTTATGGAAAGAAAAA+TGG - chr4_4:78843436-78843455 None:intergenic 20.0%
!!! TTTTCATATAATTACAGCTA+TGG + chr4_4:78843567-78843586 Msa0705980:intron 20.0%
!!! ATATACTTTTCAGATGTTAT+TGG + chr4_4:78843924-78843943 Msa0705980:intron 20.0%
!! ATGCTATGATTTAATATTGA+AGG + chr4_4:78844094-78844113 Msa0705980:intron 20.0%
!!! TTTTGACTCATTGTTTAATT+CGG - chr4_4:78844424-78844443 None:intergenic 20.0%
!! ACAATGAGTCAAAAATAATA+TGG + chr4_4:78844430-78844449 Msa0705980:intron 20.0%
!! ATGCAACAAACATTAAAAAA+TGG - chr4_4:78844491-78844510 None:intergenic 20.0%
!!! TAAATAGCTAAAATTTTGCA+AGG - chr4_4:78844564-78844583 None:intergenic 20.0%
!! ACAGTTAAAAAGTTATACAT+TGG + chr4_4:78844665-78844684 Msa0705980:three_prime_UTR 20.0%
!!! AAACTTTCATATTTTTTCCA+TGG - chr4_4:78844716-78844735 None:intergenic 20.0%
!!! TGTGTGTGATAATTTTTTTT+TGG + chr4_4:78844813-78844832 Msa0705980:three_prime_UTR 20.0%
!!! GTGTGTGATAATTTTTTTTT+GGG + chr4_4:78844814-78844833 Msa0705980:three_prime_UTR 20.0%
!!! AATGTGTTGTCTATTTTTAT+TGG + chr4_4:78844886-78844905 Msa0705980:three_prime_UTR 20.0%
!!! ATGTGTTGTCTATTTTTATT+GGG + chr4_4:78844887-78844906 Msa0705980:three_prime_UTR 20.0%
! GAATACACAAAAACAAATCA+AGG - chr4_4:78842054-78842073 None:intergenic 25.0%
!!! GATCTGAAGTTATGTTTTTT+TGG + chr4_4:78842075-78842094 Msa0705980:intron 25.0%
! ATGGTAATTATGTATGTCAT+TGG + chr4_4:78842164-78842183 Msa0705980:intron 25.0%
!!! GGAGATTTTGTTTTTTTTGT+TGG + chr4_4:78842198-78842217 Msa0705980:intron 25.0%
! GGTTTATACTTATTTGATCT+TGG + chr4_4:78842219-78842238 Msa0705980:intron 25.0%
! CAGACTAAAATGTCTTAATT+GGG + chr4_4:78842286-78842305 Msa0705980:intron 25.0%
! TTTCACAATGGATAAAATAG+AGG - chr4_4:78842340-78842359 None:intergenic 25.0%
! TACACTATCAAATTTCACAA+TGG - chr4_4:78842352-78842371 None:intergenic 25.0%
!!! ACCTTTTAGTGTAGTTTTAT+CGG + chr4_4:78842484-78842503 Msa0705980:intron 25.0%
! CTTCAACTAATATAACATGA+AGG - chr4_4:78842520-78842539 None:intergenic 25.0%
! CATGCTTAAAAAGTTAAAGA+GGG - chr4_4:78842605-78842624 None:intergenic 25.0%
! ACATGCTTAAAAAGTTAAAG+AGG - chr4_4:78842606-78842625 None:intergenic 25.0%
!!! TTTTGTTGTGTTTGTTTTGT+AGG + chr4_4:78842732-78842751 Msa0705980:intron 25.0%
!!! TTTGTTTTGTAGGTTTCTTT+TGG + chr4_4:78842742-78842761 Msa0705980:intron 25.0%
! AACCTCAAATTGATAAACTA+AGG + chr4_4:78842849-78842868 Msa0705980:three_prime_UTR 25.0%
! AACAAAATCTATAACTTGAG+CGG - chr4_4:78842969-78842988 None:intergenic 25.0%
! AGAGATAGATATAAATAGAG+AGG - chr4_4:78843034-78843053 None:intergenic 25.0%
!!! ACAGAGTGGTTTTTTAATTA+AGG - chr4_4:78843178-78843197 None:intergenic 25.0%
! ACAAGCAGTTCTTTATTATT+TGG + chr4_4:78843474-78843493 Msa0705980:intron 25.0%
! GAAATCCAATATACTTATCA+AGG - chr4_4:78844228-78844247 None:intergenic 25.0%
! AGAAACTCATTGTTTATCTT+TGG - chr4_4:78844305-78844324 None:intergenic 25.0%
!!! AAATATGAAAGTTTGTTGCA+AGG + chr4_4:78844722-78844741 Msa0705980:three_prime_UTR 25.0%
! ATTATATAATTAGATGGGCA+TGG - chr4_4:78844792-78844811 None:intergenic 25.0%
!! GGACAAGTAAAACTTTTATT+TGG + chr4_4:78844908-78844927 Msa0705980:three_prime_UTR 25.0%
AGTATTTGGGAGAGAAATAA+GGG - chr4_4:78841893-78841912 None:intergenic 30.0%
TAGTATTTGGGAGAGAAATA+AGG - chr4_4:78841894-78841913 None:intergenic 30.0%
! GATTTTTATTCTCAGCACAT+AGG + chr4_4:78841945-78841964 Msa0705980:intron 30.0%
AAACACCAACAGAAAAAAGA+GGG - chr4_4:78842134-78842153 None:intergenic 30.0%
AAAACACCAACAGAAAAAAG+AGG - chr4_4:78842135-78842154 None:intergenic 30.0%
!!! TTGGTGTTTTGTTTTCTTCA+TGG + chr4_4:78842145-78842164 Msa0705980:intron 30.0%
!! ATGTCATTGGTGATTTTGTT+TGG + chr4_4:78842177-78842196 Msa0705980:intron 30.0%
GCAGACTAAAATGTCTTAAT+TGG + chr4_4:78842285-78842304 Msa0705980:intron 30.0%
AAGTTGGAGAGAAATATAGA+AGG - chr4_4:78842671-78842690 None:intergenic 30.0%
AAGCAAAATTGCGTTAAAGT+TGG - chr4_4:78842687-78842706 None:intergenic 30.0%
CACCTTAGTTTATCAATTTG+AGG - chr4_4:78842854-78842873 None:intergenic 30.0%
!! GTTCAAATTATTGGTTCTCT+TGG + chr4_4:78842986-78843005 Msa0705980:intron 30.0%
TCTCTATATAGAGATGTGTT+TGG + chr4_4:78843049-78843068 Msa0705980:intron 30.0%
TAAGAATTAGAATAGAGGCA+TGG - chr4_4:78843136-78843155 None:intergenic 30.0%
!!! ATGAACACCTCAACTTTTTT+CGG + chr4_4:78843212-78843231 Msa0705980:intron 30.0%
! ATGCATTGTTTTCATATGAG+TGG + chr4_4:78843269-78843288 Msa0705980:intron 30.0%
! ATTGTTTTCATATGAGTGGT+TGG + chr4_4:78843273-78843292 Msa0705980:intron 30.0%
GCTACACAGATATTTCATAT+TGG - chr4_4:78843316-78843335 None:intergenic 30.0%
GCAATCAATAGAAATTGAAG+AGG - chr4_4:78843357-78843376 None:intergenic 30.0%
AGAAGGGAAAAAATGAATGA+AGG - chr4_4:78843411-78843430 None:intergenic 30.0%
GGAAAGAAAAATGGTTAGAA+GGG - chr4_4:78843427-78843446 None:intergenic 30.0%
TGGAAAGAAAAATGGTTAGA+AGG - chr4_4:78843428-78843447 None:intergenic 30.0%
AATAAAGAACTGCTTGTACA+TGG - chr4_4:78843471-78843490 None:intergenic 30.0%
TGAACTAACGACTTAATCTT+TGG - chr4_4:78843626-78843645 None:intergenic 30.0%
ATTAAGTCGTTAGTTCAAGA+AGG + chr4_4:78843629-78843648 Msa0705980:three_prime_UTR 30.0%
TCAAGGACAGTTTAAATCTA+TGG - chr4_4:78843690-78843709 None:intergenic 30.0%
ACGAAATAAAGATGGTTAGA+AGG - chr4_4:78843754-78843773 None:intergenic 30.0%
TGAAAGTCACGAAATAAAGA+TGG - chr4_4:78843762-78843781 None:intergenic 30.0%
! TCTTTATTTCGTGACTTTCA+AGG + chr4_4:78843762-78843781 Msa0705980:three_prime_UTR 30.0%
AAGAGAGAGAATAAGTACTT+AGG + chr4_4:78843828-78843847 Msa0705980:three_prime_UTR 30.0%
AGAGAATAAGTACTTAGGTA+AGG + chr4_4:78843833-78843852 Msa0705980:intron 30.0%
AATATTAAACCACAGTATCC+TGG - chr4_4:78844135-78844154 None:intergenic 30.0%
!! TTACCTTAGTTCTAATGATG+TGG + chr4_4:78844393-78844412 Msa0705980:intron 30.0%
! ATAGCTAAAATTTTGCAAGG+AGG - chr4_4:78844561-78844580 None:intergenic 30.0%
! ATACATTGGCTGAAATTGAA+AGG + chr4_4:78844679-78844698 Msa0705980:three_prime_UTR 30.0%
TGAGAGAGTTTATTGGAAAG+TGG - chr4_4:78841520-78841539 None:intergenic 35.0%
TTCAGTTTCGAGGTTTGATT+CGG - chr4_4:78841726-78841745 None:intergenic 35.0%
!!! TGAATCCCTCTTTTTTCTGT+TGG + chr4_4:78842126-78842145 Msa0705980:intron 35.0%
GCCGATAAAACTACACTAAA+AGG - chr4_4:78842488-78842507 None:intergenic 35.0%
AAGAGCAGTTGAATCTGAAT+TGG + chr4_4:78842805-78842824 Msa0705980:three_prime_UTR 35.0%
AGAGCAGTTGAATCTGAATT+GGG + chr4_4:78842806-78842825 Msa0705980:three_prime_UTR 35.0%
GAAATCTTGATGAGATAGCT+AGG + chr4_4:78842918-78842937 Msa0705980:intron 35.0%
TGTTGAACACATTGTGATGA+GGG - chr4_4:78843240-78843259 None:intergenic 35.0%
TTGTTGAACACATTGTGATG+AGG - chr4_4:78843241-78843260 None:intergenic 35.0%
GAGGCATGAACACAAATAAT+TGG - chr4_4:78843338-78843357 None:intergenic 35.0%
!! TTAGTCCAGTCGTTATTTTG+TGG + chr4_4:78843516-78843535 Msa0705980:intron 35.0%
!! TAGTCCAGTCGTTATTTTGT+GGG + chr4_4:78843517-78843536 Msa0705980:intron 35.0%
!! TCGTTATTTTGTGGGGTAAA+AGG + chr4_4:78843525-78843544 Msa0705980:intron 35.0%
AGAAGGTTCCAATTATGTTG+TGG + chr4_4:78843646-78843665 Msa0705980:three_prime_UTR 35.0%
GGTATCTTCCACAACATAAT+TGG - chr4_4:78843657-78843676 None:intergenic 35.0%
TTAAATCTATGGCCAGTAAG+CGG - chr4_4:78843679-78843698 None:intergenic 35.0%
TTCTTCAACTTTGGTCCAAA+TGG - chr4_4:78843722-78843741 None:intergenic 35.0%
CATCAGACTTTCTTCAACTT+TGG - chr4_4:78843731-78843750 None:intergenic 35.0%
AGATGTTATTGGACATGTTG+TGG + chr4_4:78843935-78843954 Msa0705980:intron 35.0%
TTATTGGACATGTTGTGGAA+AGG + chr4_4:78843940-78843959 Msa0705980:three_prime_UTR 35.0%
TATTGGACATGTTGTGGAAA+GGG + chr4_4:78843941-78843960 Msa0705980:three_prime_UTR 35.0%
!! GTTGGCCTTGATAAGTATAT+TGG + chr4_4:78844220-78844239 Msa0705980:intron 35.0%
ACTCATTGTTTAATTCGGCT+CGG - chr4_4:78844419-78844438 None:intergenic 35.0%
GAAATTGAAAGGTTGTTGCA+AGG + chr4_4:78844690-78844709 Msa0705980:three_prime_UTR 35.0%
TGGAAAGTGGAAGAGAAGAT+CGG - chr4_4:78841507-78841526 None:intergenic 40.0%
GAGAGACTGAGAGAGTTTAT+TGG - chr4_4:78841527-78841546 None:intergenic 40.0%
TTCAGTGTCATCTTCGTTGA+AGG - chr4_4:78841667-78841686 None:intergenic 40.0%
CATCTCCATCCTTCGATAAT+TGG + chr4_4:78841697-78841716 Msa0705980:five_prime_UTR 40.0%
ATTCGGTTGCCAATTATCGA+AGG - chr4_4:78841709-78841728 None:intergenic 40.0%
CAATTTCGCATTCAGTTTCG+AGG - chr4_4:78841736-78841755 None:intergenic 40.0%
CTCGAAACTGAATGCGAAAT+TGG + chr4_4:78841734-78841753 Msa0705980:five_prime_UTR 40.0%
ACATTGATGAGGTAGCAGTA+GGG - chr4_4:78842385-78842404 None:intergenic 40.0%
AACATTGATGAGGTAGCAGT+AGG - chr4_4:78842386-78842405 None:intergenic 40.0%
! TGATAGTGTAACCTCACTGA+AGG + chr4_4:78842426-78842445 Msa0705980:intron 40.0%
TGATCAAGTGTCATCTGAGT+CGG - chr4_4:78842460-78842479 None:intergenic 40.0%
!!! TTGAGCGGCATTTTTTTTCG+TGG - chr4_4:78842954-78842973 None:intergenic 40.0%
!! AGTCCAGTCGTTATTTTGTG+GGG + chr4_4:78843518-78843537 Msa0705980:intron 40.0%
TTACCCCACAAAATAACGAC+TGG - chr4_4:78843524-78843543 None:intergenic 40.0%
TAAATCTATGGCCAGTAAGC+GGG - chr4_4:78843678-78843697 None:intergenic 40.0%
CCAAATGGTTCGATCAATCA+AGG - chr4_4:78843707-78843726 None:intergenic 40.0%
CCTTGATTGATCGAACCATT+TGG + chr4_4:78843704-78843723 Msa0705980:three_prime_UTR 40.0%
AGTACTTAGGTAAGGCAGAT+TGG + chr4_4:78843841-78843860 Msa0705980:intron 40.0%
CAAGCTGCTTGACATTACAT+TGG + chr4_4:78844001-78844020 Msa0705980:three_prime_UTR 40.0%
ACAGTATCCTGGTATACACA+TGG - chr4_4:78844124-78844143 None:intergenic 40.0%
ATGTGTATACCAGGATACTG+TGG + chr4_4:78844123-78844142 Msa0705980:intron 40.0%
! TCTTTGGTGTAAGTCTGAGT+GGG - chr4_4:78844289-78844308 None:intergenic 40.0%
! ATCTTTGGTGTAAGTCTGAG+TGG - chr4_4:78844290-78844309 None:intergenic 40.0%
GAGATGTTGAAACATCGTTC+TGG - chr4_4:78844518-78844537 None:intergenic 40.0%
AGAAGATCGGTGGAGTGTAT+TGG - chr4_4:78841494-78841513 None:intergenic 45.0%
AAAGTGGAAGAGAAGATCGG+TGG - chr4_4:78841504-78841523 None:intergenic 45.0%
!! CATCTTCGTTGAAGGTGCTA+CGG - chr4_4:78841659-78841678 None:intergenic 45.0%
GGTTGCCAATTATCGAAGGA+TGG - chr4_4:78841705-78841724 None:intergenic 45.0%
GCTTTCCGATCTTCTGAACA+TGG + chr4_4:78841761-78841780 Msa0705980:exon 45.0%
TTCCGATCTTCTGAACATGG+CGG + chr4_4:78841764-78841783 Msa0705980:exon 45.0%
TCTTCCTTCTTCTTCGTCTC+CGG - chr4_4:78841812-78841831 None:intergenic 45.0%
GAAGAAGAAGGAAGAAGCCT+TGG + chr4_4:78841817-78841836 Msa0705980:CDS 45.0%
TAGAGGAATGCAGTCACACA+TGG - chr4_4:78842323-78842342 None:intergenic 45.0%
CGGTCAAATTTCCTTCAGTG+AGG - chr4_4:78842440-78842459 None:intergenic 45.0%
!! GGTTTCTTTTGGTTGCTGCT+TGG + chr4_4:78842753-78842772 Msa0705980:intron 45.0%
AGAGTGTGCATCTTACAGAG+TGG - chr4_4:78843192-78843211 None:intergenic 45.0%
GAGGGAACCGAAAAAAGTTG+AGG - chr4_4:78843222-78843241 None:intergenic 45.0%
TTTCAAGGATGTCCTGGACT+CGG + chr4_4:78843777-78843796 Msa0705980:three_prime_UTR 45.0%
! ATGTCAAGCAGCTTGCTCTT+AGG - chr4_4:78843996-78844015 None:intergenic 45.0%
CGGCCACATCATTAGAACTA+AGG - chr4_4:78844399-78844418 None:intergenic 45.0%
!! GGGCGGTAGTGGAAGAATAT+TGG - chr4_4:78841553-78841572 None:intergenic 50.0%
AGGCAATGGAGGGAGAGTTA+CGG - chr4_4:78841576-78841595 None:intergenic 50.0%
GTGGAAGATGATACGACGGA+GGG - chr4_4:78841599-78841618 None:intergenic 50.0%
AACGTGATCACCGCAACTGA+CGG - chr4_4:78841623-78841642 None:intergenic 50.0%
CTCCGCCATGTTCAGAAGAT+CGG - chr4_4:78841769-78841788 None:intergenic 50.0%
GAAGAAGGAAGAAGCCTTGG+AGG + chr4_4:78841820-78841839 Msa0705980:CDS 50.0%
! GAACGCTTTGAGTTCCTCCA+AGG - chr4_4:78841837-78841856 None:intergenic 50.0%
ACGTTACCAGGGAAGGTTGA+GGG - chr4_4:78841865-78841884 None:intergenic 50.0%
TAAGGGTTGCGACGTTACCA+GGG - chr4_4:78841876-78841895 None:intergenic 50.0%
ATAAGGGTTGCGACGTTACC+AGG - chr4_4:78841877-78841896 None:intergenic 50.0%
GGTAGCAGTAGGGCGATAAA+AGG - chr4_4:78842375-78842394 None:intergenic 50.0%
!! AGCGACTGCGAAAAAGCATC+TGG + chr4_4:78843598-78843617 Msa0705980:three_prime_UTR 50.0%
TGTGGAAGATACCCGCTTAC+TGG + chr4_4:78843664-78843683 Msa0705980:three_prime_UTR 50.0%
CGTGACTTTCAAGGATGTCC+TGG + chr4_4:78843771-78843790 Msa0705980:three_prime_UTR 50.0%
TATTCTCTCTGTCCGAGTCC+AGG - chr4_4:78843792-78843811 None:intergenic 50.0%
GAAAGGTTGTTGCAAGGCCA+TGG + chr4_4:78844696-78844715 Msa0705980:three_prime_UTR 50.0%
CAATGGAGGGAGAGTTACGG+GGG - chr4_4:78841573-78841592 None:intergenic 55.0%
GCAATGGAGGGAGAGTTACG+GGG - chr4_4:78841574-78841593 None:intergenic 55.0%
GGCAATGGAGGGAGAGTTAC+GGG - chr4_4:78841575-78841594 None:intergenic 55.0%
GAAGATGATACGACGGAGGG+AGG - chr4_4:78841596-78841615 None:intergenic 55.0%
CGTGGAAGATGATACGACGG+AGG - chr4_4:78841600-78841619 None:intergenic 55.0%
CGGCGTGGAAGATGATACGA+CGG - chr4_4:78841603-78841622 None:intergenic 55.0%
ATCTTCCACGCCGTCAGTTG+CGG + chr4_4:78841610-78841629 Msa0705980:five_prime_UTR 55.0%
CGATCTTCTGAACATGGCGG+AGG + chr4_4:78841767-78841786 Msa0705980:exon 55.0%
! TCTTCTGAACATGGCGGAGG+TGG + chr4_4:78841770-78841789 Msa0705980:exon 55.0%
! CTTCTGAACATGGCGGAGGT+GGG + chr4_4:78841771-78841790 Msa0705980:exon 55.0%
! TTCTGAACATGGCGGAGGTG+GGG + chr4_4:78841772-78841791 Msa0705980:exon 55.0%
CGTTACCAGGGAAGGTTGAG+GGG - chr4_4:78841864-78841883 None:intergenic 55.0%
GACGTTACCAGGGAAGGTTG+AGG - chr4_4:78841866-78841885 None:intergenic 55.0%
AGGCGTGCCATGTGTATACC+AGG + chr4_4:78844114-78844133 Msa0705980:intron 55.0%
GATACGACGGAGGGAGGCAA+TGG - chr4_4:78841590-78841609 None:intergenic 60.0%
!! CGTTGAAGGTGCTACGGCAG+CGG - chr4_4:78841653-78841672 None:intergenic 60.0%
! TCTGAACATGGCGGAGGTGG+GGG + chr4_4:78841773-78841792 Msa0705980:exon 60.0%
AGGGCCGGAGACGAAGAAGA+AGG + chr4_4:78841805-78841824 Msa0705980:CDS 60.0%
CTCTTCCCCTCAACCTTCCC+TGG + chr4_4:78841856-78841875 Msa0705980:CDS 60.0%
GGTTGCGACGTTACCAGGGA+AGG - chr4_4:78841872-78841891 None:intergenic 60.0%
GAGAGTTACGGGGGCGGTAG+TGG - chr4_4:78841564-78841583 None:intergenic 65.0%
TGGAGGGAGAGTTACGGGGG+CGG - chr4_4:78841570-78841589 None:intergenic 65.0%
CGACGGAGGGAGGCAATGGA+GGG - chr4_4:78841586-78841605 None:intergenic 65.0%
ACGACGGAGGGAGGCAATGG+AGG - chr4_4:78841587-78841606 None:intergenic 65.0%
GATCACCGCAACTGACGGCG+TGG - chr4_4:78841618-78841637 None:intergenic 65.0%
GAGGTGGGGGCAGAAGCGGA+GGG + chr4_4:78841786-78841805 Msa0705980:CDS 70.0%
GGCGGAGGTGGGGGCAGAAG+CGG + chr4_4:78841782-78841801 Msa0705980:CDS 75.0%
GGAGGTGGGGGCAGAAGCGG+AGG + chr4_4:78841785-78841804 Msa0705980:CDS 75.0%
TGGGGGCAGAAGCGGAGGGC+CGG + chr4_4:78841790-78841809 Msa0705980:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 78841485 78844931 78841485 ID=Msa0705980;Name=Msa0705980
chr4_4 mRNA 78841485 78844931 78841485 ID=Msa0705980-mRNA-1;Parent=Msa0705980;Name=Msa0705980-mRNA-1;_AED=0.08;_eAED=0.08;_QI=295|1|1|1|0.25|0.4|5|739|41
chr4_4 exon 78841485 78841877 78841485 ID=Msa0705980-mRNA-1:exon:25239;Parent=Msa0705980-mRNA-1
chr4_4 exon 78842754 78842870 78842754 ID=Msa0705980-mRNA-1:exon:25240;Parent=Msa0705980-mRNA-1
chr4_4 exon 78843584 78843849 78843584 ID=Msa0705980-mRNA-1:exon:25241;Parent=Msa0705980-mRNA-1
chr4_4 exon 78843937 78844033 78843937 ID=Msa0705980-mRNA-1:exon:25242;Parent=Msa0705980-mRNA-1
chr4_4 exon 78844645 78844931 78844645 ID=Msa0705980-mRNA-1:exon:25243;Parent=Msa0705980-mRNA-1
chr4_4 five_prime_UTR 78841485 78841779 78841485 ID=Msa0705980-mRNA-1:five_prime_utr;Parent=Msa0705980-mRNA-1
chr4_4 CDS 78841780 78841877 78841780 ID=Msa0705980-mRNA-1:cds;Parent=Msa0705980-mRNA-1
chr4_4 CDS 78842754 78842781 78842754 ID=Msa0705980-mRNA-1:cds;Parent=Msa0705980-mRNA-1
chr4_4 three_prime_UTR 78842782 78842870 78842782 ID=Msa0705980-mRNA-1:three_prime_utr;Parent=Msa0705980-mRNA-1
chr4_4 three_prime_UTR 78843584 78843849 78843584 ID=Msa0705980-mRNA-1:three_prime_utr;Parent=Msa0705980-mRNA-1
chr4_4 three_prime_UTR 78843937 78844033 78843937 ID=Msa0705980-mRNA-1:three_prime_utr;Parent=Msa0705980-mRNA-1
chr4_4 three_prime_UTR 78844645 78844931 78844645 ID=Msa0705980-mRNA-1:three_prime_utr;Parent=Msa0705980-mRNA-1
Gene Sequence

>Msa0705980

ATGGCGGAGGTGGGGGCAGAAGCGGAGGGCCGGAGACGAAGAAGAAGGAAGAAGCCTTGGAGGAACTCAAAGCGTTCTCTTCCCCTCAACCTTCCCTGGTTTCTTTTGGTTGCTGCTTGGTGTTGA
Protein sequence

>Msa0705980

MAEVGAEAEGRRRRRRKKPWRNSKRSLPLNLPWFLLVAAWC*