AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708030 MtrunA17_Chr5g0432621 87.333 150 19 0 3 152 25 174 2.96e-101 287
Msa0708030 MtrunA17_Chr8g0390661 98.246 114 2 0 39 152 1 114 1.81e-83 240
Msa0708030 MtrunA17_Chr8g0385351 28.873 142 84 3 18 143 28 168 8.06e-15 67.8
Msa0708030 MtrunA17_Chr4g0024741 29.787 141 84 3 18 143 26 166 1.16e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708030 AT5G02280.1 87.943 141 17 0 12 152 1 141 4.22e-94 268
Msa0708030 AT1G51160.1 29.078 141 85 3 18 143 26 166 6.03e-14 65.9
Msa0708030 AT1G51160.3 29.078 141 85 3 18 143 26 166 6.03e-14 65.9
Msa0708030 AT1G51160.2 29.078 141 85 3 18 143 26 166 6.03e-14 65.9

Find 46 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCAGTTGTCACCTGTTTC+TGG 0.050534 4_4:-81484744 Msa0708030:CDS
TTTCCTGATATTTAGGATTA+TGG 0.113471 4_4:-81484840 Msa0708030:intron
TTCAGGTCAATTTATCAATT+TGG 0.143355 4_4:+81483788 None:intergenic
ATTTAGGATTATGGGTCTTC+TGG 0.243224 4_4:-81484831 Msa0708030:intron
CTTTCCATTTGTTGAGTTCC+AGG 0.279445 4_4:+81484142 None:intergenic
TTCCTGATATTTAGGATTAT+GGG 0.283975 4_4:-81484839 Msa0708030:intron
CAGATTCTTGCAAAGGCTTT+GGG 0.307507 4_4:-81485526 Msa0708030:CDS
TCAGATTCTTGCAAAGGCTT+TGG 0.323137 4_4:-81485527 Msa0708030:CDS
CTAGCAGGTAACATGTAATT+TGG 0.325761 4_4:-81483830 Msa0708030:three_prime_UTR
GAAGGACCGTGTTGCATTGC+TGG 0.349606 4_4:-81484002 Msa0708030:CDS
ATGAAAATATAAGCAACATT+TGG 0.375141 4_4:-81483757 Msa0708030:three_prime_UTR
AAATGACGAAAAGCATATAT+TGG 0.396478 4_4:+81483881 None:intergenic
CGGTGGATTGATCTACTATA+AGG 0.397918 4_4:-81485464 Msa0708030:intron
AGGCATCTCCATCTCATAGA+AGG 0.407862 4_4:+81484069 None:intergenic
TCGATAAACTGCCATGGATC+TGG 0.421423 4_4:-81483978 Msa0708030:exon
GCTTACGGGTGGCTAGCTTA+TGG 0.427608 4_4:-81484788 Msa0708030:CDS
AAATAATACCTGTCAGTGAC+TGG 0.443726 4_4:+81484670 None:intergenic
ATTCCTGCGCAACCAGAAAC+AGG 0.450165 4_4:+81484732 None:intergenic
GTAACATGTAATTTGGTACT+TGG 0.454701 4_4:-81483823 Msa0708030:three_prime_UTR
TGAACCTGGAACTCAACAAA+TGG 0.475337 4_4:-81484146 Msa0708030:CDS
TCAAAGAGCTCACATCGTAT+AGG 0.480843 4_4:+81484049 None:intergenic
GGACACCAATGATAGCTTAC+GGG 0.481121 4_4:-81484802 Msa0708030:CDS
CATTGCTTCCAGTCACTGAC+AGG 0.492920 4_4:-81484678 Msa0708030:intron
GGCATCTCCATCTCATAGAA+GGG 0.506472 4_4:+81484070 None:intergenic
GGCATTTCAGATTCTTGCAA+AGG 0.507176 4_4:-81485533 Msa0708030:CDS
AGCCACCCGTAAGCTATCAT+TGG 0.510288 4_4:+81484797 None:intergenic
TGGACACCAATGATAGCTTA+CGG 0.512682 4_4:-81484803 Msa0708030:CDS
TCACCTGTTTCTGGTTGCGC+AGG 0.516213 4_4:-81484735 Msa0708030:CDS
CTTTGATATCAACTTAGCTC+AGG 0.519060 4_4:-81484032 Msa0708030:CDS
AAGGACCGTGTTGCATTGCT+GGG 0.527169 4_4:-81484001 Msa0708030:CDS
GAAGATCAAATGTGTCAGCT+TGG 0.530412 4_4:+81484700 None:intergenic
CTTTACATCATCAATAAGTC+CGG 0.534062 4_4:-81485484 Msa0708030:CDS
CTTAGCTCAGGCAGTGCAGA+AGG 0.554879 4_4:-81484020 Msa0708030:CDS
TTATGGGTCTTCTGGACGAA+TGG 0.565210 4_4:-81484823 Msa0708030:CDS
AAATTGATAAATTGACCTGA+AGG 0.566402 4_4:-81483786 Msa0708030:three_prime_UTR
GAAGAACCCCTTCTATGAGA+TGG 0.572651 4_4:-81484077 Msa0708030:CDS
TAAGTTAAATTGCTTAAACA+TGG 0.573495 4_4:-81485554 None:intergenic
AACAACACTTGCCAGATCCA+TGG 0.576950 4_4:+81483967 None:intergenic
TACATCATCAATAAGTCCGG+TGG 0.589559 4_4:-81485481 Msa0708030:CDS
AAATTAAACATACATCTAGC+AGG 0.595746 4_4:-81483845 Msa0708030:three_prime_UTR
AGGTGACAACTGCTGAGAGA+TGG 0.604194 4_4:+81484752 None:intergenic
GCTGGGTCGATAAACTGCCA+TGG 0.619651 4_4:-81483984 Msa0708030:exon
GCATCTCCATCTCATAGAAG+GGG 0.643808 4_4:+81484071 None:intergenic
CACCAATGATAGCTTACGGG+TGG 0.665422 4_4:-81484799 Msa0708030:CDS
CTTATAGTAGATCAATCCAC+CGG 0.673585 4_4:+81485465 None:intergenic
ATCGACCCAGCAATGCAACA+CGG 0.698434 4_4:+81483996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAACAATACTAAAAAATTT+AGG - chr4_4:81484087-81484106 Msa0708030:CDS 10.0%
!!! TTTTTAAAAAATTGTTATAG+TGG - chr4_4:81485356-81485375 Msa0708030:intron 10.0%
!! TAAACAATACTAAAAAATTT+AGG - chr4_4:81484087-81484106 Msa0708030:CDS 10.0%
!!! TTTTTAAAAAATTGTTATAG+TGG - chr4_4:81485356-81485375 Msa0708030:intron 10.0%
!!! TTATTTTTATTGTAAGCTTA+AGG + chr4_4:81484198-81484217 None:intergenic 15.0%
!! GAAAGTTTAGTAATAATTAT+TGG - chr4_4:81484853-81484872 Msa0708030:intron 15.0%
!!! TGCTTATAATACAAAATTTT+AGG - chr4_4:81485395-81485414 Msa0708030:intron 15.0%
!!! TTATTTTTATTGTAAGCTTA+AGG + chr4_4:81484198-81484217 None:intergenic 15.0%
!! GAAAGTTTAGTAATAATTAT+TGG - chr4_4:81484853-81484872 Msa0708030:intron 15.0%
!!! TGCTTATAATACAAAATTTT+AGG - chr4_4:81485395-81485414 Msa0708030:intron 15.0%
!!! TTCAGTAAAACATTTTTCAT+AGG + chr4_4:81483935-81483954 None:intergenic 20.0%
!!! TGTTTAATTTCCTGATATTT+AGG - chr4_4:81484455-81484474 Msa0708030:intron 20.0%
!! CTTAACATAATAATGAGTTA+TGG + chr4_4:81484659-81484678 None:intergenic 20.0%
!!! TGTTTAATTTTGTAGAGATT+AGG + chr4_4:81485447-81485466 None:intergenic 20.0%
!! ATGAAAATATAAGCAACATT+TGG - chr4_4:81485545-81485564 Msa0708030:exon 20.0%
!!! TTCAGTAAAACATTTTTCAT+AGG + chr4_4:81483935-81483954 None:intergenic 20.0%
!!! TGTTTAATTTCCTGATATTT+AGG - chr4_4:81484455-81484474 Msa0708030:intron 20.0%
!! CTTAACATAATAATGAGTTA+TGG + chr4_4:81484659-81484678 None:intergenic 20.0%
!!! TGTTTAATTTTGTAGAGATT+AGG + chr4_4:81485447-81485466 None:intergenic 20.0%
!!! TGATTTTTGGTTTTAGGATA+TGG - chr4_4:81484130-81484149 Msa0708030:CDS 25.0%
! AAAAACTTTCTGAGTTAGAT+GGG + chr4_4:81484362-81484381 None:intergenic 25.0%
! TAAAAACTTTCTGAGTTAGA+TGG + chr4_4:81484363-81484382 None:intergenic 25.0%
!! AAAGTTTTTACTACTGTTGT+TGG - chr4_4:81484373-81484392 Msa0708030:intron 25.0%
!! TTTCCTGATATTTAGGATTA+TGG - chr4_4:81484462-81484481 Msa0708030:intron 25.0%
!! TTCCTGATATTTAGGATTAT+GGG - chr4_4:81484463-81484482 Msa0708030:intron 25.0%
! TGTGAATAAAAAGCAAACAT+TGG + chr4_4:81484760-81484779 None:intergenic 25.0%
!!! TGTTTGCTTTTTATTCACAT+TGG - chr4_4:81484761-81484780 Msa0708030:CDS 25.0%
! GGTAAGAATTCAAACATAAT+TGG + chr4_4:81484960-81484979 None:intergenic 25.0%
!! ATTTAAAATCTACTAGTGCT+AGG + chr4_4:81485083-81485102 None:intergenic 25.0%
!! AAATGACGAAAAGCATATAT+TGG + chr4_4:81485424-81485443 None:intergenic 25.0%
! AAATTAAACATACATCTAGC+AGG - chr4_4:81485457-81485476 Msa0708030:intron 25.0%
! TTCAGGTCAATTTATCAATT+TGG + chr4_4:81485517-81485536 None:intergenic 25.0%
! AAATTGATAAATTGACCTGA+AGG - chr4_4:81485516-81485535 Msa0708030:CDS 25.0%
!!! TGATTTTTGGTTTTAGGATA+TGG - chr4_4:81484130-81484149 Msa0708030:CDS 25.0%
! AAAAACTTTCTGAGTTAGAT+GGG + chr4_4:81484362-81484381 None:intergenic 25.0%
! TAAAAACTTTCTGAGTTAGA+TGG + chr4_4:81484363-81484382 None:intergenic 25.0%
!! AAAGTTTTTACTACTGTTGT+TGG - chr4_4:81484373-81484392 Msa0708030:intron 25.0%
!! TTTCCTGATATTTAGGATTA+TGG - chr4_4:81484462-81484481 Msa0708030:intron 25.0%
!! TTCCTGATATTTAGGATTAT+GGG - chr4_4:81484463-81484482 Msa0708030:intron 25.0%
! TGTGAATAAAAAGCAAACAT+TGG + chr4_4:81484760-81484779 None:intergenic 25.0%
!!! TGTTTGCTTTTTATTCACAT+TGG - chr4_4:81484761-81484780 Msa0708030:CDS 25.0%
! GGTAAGAATTCAAACATAAT+TGG + chr4_4:81484960-81484979 None:intergenic 25.0%
!! ATTTAAAATCTACTAGTGCT+AGG + chr4_4:81485083-81485102 None:intergenic 25.0%
!! AAATGACGAAAAGCATATAT+TGG + chr4_4:81485424-81485443 None:intergenic 25.0%
! AAATTAAACATACATCTAGC+AGG - chr4_4:81485457-81485476 Msa0708030:intron 25.0%
! TTCAGGTCAATTTATCAATT+TGG + chr4_4:81485517-81485536 None:intergenic 25.0%
! AAATTGATAAATTGACCTGA+AGG - chr4_4:81485516-81485535 Msa0708030:CDS 25.0%
CTTTACATCATCAATAAGTC+CGG - chr4_4:81483818-81483837 Msa0708030:three_prime_UTR 30.0%
! AATAAGTTGGGTTGTTAATC+AGG - chr4_4:81483976-81483995 Msa0708030:exon 30.0%
!!! CTATTCACGTCATTGATTTT+TGG - chr4_4:81484117-81484136 Msa0708030:CDS 30.0%
!!! CGTCATTGATTTTTGGTTTT+AGG - chr4_4:81484124-81484143 Msa0708030:CDS 30.0%
AAGAATGAATGCCAAAAATC+TGG - chr4_4:81484706-81484725 Msa0708030:CDS 30.0%
! AGTGATATGTACCAGATTTT+TGG + chr4_4:81484720-81484739 None:intergenic 30.0%
! CTGGGTTTTACTAACATTTA+AGG - chr4_4:81484827-81484846 Msa0708030:CDS 30.0%
TACATACAGGTAAAATTCCA+AGG + chr4_4:81484896-81484915 None:intergenic 30.0%
! CAAACATAATTGGTAACAAC+TGG + chr4_4:81484950-81484969 None:intergenic 30.0%
CAATTAAGGTGACAAATCTA+CGG + chr4_4:81484981-81485000 None:intergenic 30.0%
GTAACATGTAATTTGGTACT+TGG - chr4_4:81485479-81485498 Msa0708030:CDS 30.0%
! TATTTTCATTAAGCACCTTC+AGG + chr4_4:81485534-81485553 None:intergenic 30.0%
CTTTACATCATCAATAAGTC+CGG - chr4_4:81483818-81483837 Msa0708030:three_prime_UTR 30.0%
! AATAAGTTGGGTTGTTAATC+AGG - chr4_4:81483976-81483995 Msa0708030:exon 30.0%
!!! CTATTCACGTCATTGATTTT+TGG - chr4_4:81484117-81484136 Msa0708030:CDS 30.0%
!!! CGTCATTGATTTTTGGTTTT+AGG - chr4_4:81484124-81484143 Msa0708030:CDS 30.0%
AAGAATGAATGCCAAAAATC+TGG - chr4_4:81484706-81484725 Msa0708030:CDS 30.0%
! AGTGATATGTACCAGATTTT+TGG + chr4_4:81484720-81484739 None:intergenic 30.0%
! CTGGGTTTTACTAACATTTA+AGG - chr4_4:81484827-81484846 Msa0708030:CDS 30.0%
TACATACAGGTAAAATTCCA+AGG + chr4_4:81484896-81484915 None:intergenic 30.0%
! CAAACATAATTGGTAACAAC+TGG + chr4_4:81484950-81484969 None:intergenic 30.0%
CAATTAAGGTGACAAATCTA+CGG + chr4_4:81484981-81485000 None:intergenic 30.0%
GTAACATGTAATTTGGTACT+TGG - chr4_4:81485479-81485498 Msa0708030:CDS 30.0%
! TATTTTCATTAAGCACCTTC+AGG + chr4_4:81485534-81485553 None:intergenic 30.0%
CTTATAGTAGATCAATCCAC+CGG + chr4_4:81483840-81483859 None:intergenic 35.0%
CCCAACTTATTCATCAAAGA+GGG + chr4_4:81483967-81483986 None:intergenic 35.0%
CCCTCTTTGATGAATAAGTT+GGG - chr4_4:81483964-81483983 Msa0708030:three_prime_UTR 35.0%
ACCCAACTTATTCATCAAAG+AGG + chr4_4:81483968-81483987 None:intergenic 35.0%
TTAATCAGGTCACTTGAACT+TGG - chr4_4:81483990-81484009 Msa0708030:exon 35.0%
TCACGTCGTAGATGAAAATA+AGG - chr4_4:81484155-81484174 Msa0708030:CDS 35.0%
TTTGTCAAGGCTGTTAATCA+AGG + chr4_4:81484301-81484320 None:intergenic 35.0%
GACCCATAATCCTAAATATC+AGG + chr4_4:81484468-81484487 None:intergenic 35.0%
! ATTTAGGATTATGGGTCTTC+TGG - chr4_4:81484471-81484490 Msa0708030:intron 35.0%
AAATAATACCTGTCAGTGAC+TGG + chr4_4:81484635-81484654 None:intergenic 35.0%
ATCTGGTACATATCACTGAT+TGG - chr4_4:81484723-81484742 Msa0708030:CDS 35.0%
TTCACATTGGTAAGTAACCT+TGG - chr4_4:81484774-81484793 Msa0708030:CDS 35.0%
!! TAACATAGTCTTATGTGCCT+TGG - chr4_4:81484876-81484895 Msa0708030:intron 35.0%
TTTACCTGTATGTAATGCCT+TGG - chr4_4:81484902-81484921 Msa0708030:intron 35.0%
TGTAATGCCTTGGTCATTTA+AGG - chr4_4:81484912-81484931 Msa0708030:intron 35.0%
CGTAGATTTGTCACCTTAAT+TGG - chr4_4:81484979-81484998 Msa0708030:intron 35.0%
TACTAGTGCTAGGTTATAGA+TGG + chr4_4:81485073-81485092 None:intergenic 35.0%
AATTAATTCTTCTCTGCCAC+AGG - chr4_4:81485112-81485131 Msa0708030:intron 35.0%
ATTAATTCTTCTCTGCCACA+GGG - chr4_4:81485113-81485132 Msa0708030:intron 35.0%
CTTTGATATCAACTTAGCTC+AGG - chr4_4:81485270-81485289 Msa0708030:intron 35.0%
CTAGCAGGTAACATGTAATT+TGG - chr4_4:81485472-81485491 Msa0708030:CDS 35.0%
CTTATAGTAGATCAATCCAC+CGG + chr4_4:81483840-81483859 None:intergenic 35.0%
CCCAACTTATTCATCAAAGA+GGG + chr4_4:81483967-81483986 None:intergenic 35.0%
CCCTCTTTGATGAATAAGTT+GGG - chr4_4:81483964-81483983 Msa0708030:three_prime_UTR 35.0%
ACCCAACTTATTCATCAAAG+AGG + chr4_4:81483968-81483987 None:intergenic 35.0%
TTAATCAGGTCACTTGAACT+TGG - chr4_4:81483990-81484009 Msa0708030:exon 35.0%
TCACGTCGTAGATGAAAATA+AGG - chr4_4:81484155-81484174 Msa0708030:CDS 35.0%
TTTGTCAAGGCTGTTAATCA+AGG + chr4_4:81484301-81484320 None:intergenic 35.0%
GACCCATAATCCTAAATATC+AGG + chr4_4:81484468-81484487 None:intergenic 35.0%
! ATTTAGGATTATGGGTCTTC+TGG - chr4_4:81484471-81484490 Msa0708030:intron 35.0%
AAATAATACCTGTCAGTGAC+TGG + chr4_4:81484635-81484654 None:intergenic 35.0%
ATCTGGTACATATCACTGAT+TGG - chr4_4:81484723-81484742 Msa0708030:CDS 35.0%
TTCACATTGGTAAGTAACCT+TGG - chr4_4:81484774-81484793 Msa0708030:CDS 35.0%
!! TAACATAGTCTTATGTGCCT+TGG - chr4_4:81484876-81484895 Msa0708030:intron 35.0%
TTTACCTGTATGTAATGCCT+TGG - chr4_4:81484902-81484921 Msa0708030:intron 35.0%
TGTAATGCCTTGGTCATTTA+AGG - chr4_4:81484912-81484931 Msa0708030:intron 35.0%
CGTAGATTTGTCACCTTAAT+TGG - chr4_4:81484979-81484998 Msa0708030:intron 35.0%
TACTAGTGCTAGGTTATAGA+TGG + chr4_4:81485073-81485092 None:intergenic 35.0%
AATTAATTCTTCTCTGCCAC+AGG - chr4_4:81485112-81485131 Msa0708030:intron 35.0%
ATTAATTCTTCTCTGCCACA+GGG - chr4_4:81485113-81485132 Msa0708030:intron 35.0%
CTTTGATATCAACTTAGCTC+AGG - chr4_4:81485270-81485289 Msa0708030:intron 35.0%
CTAGCAGGTAACATGTAATT+TGG - chr4_4:81485472-81485491 Msa0708030:CDS 35.0%
GGCATTTCAGATTCTTGCAA+AGG - chr4_4:81483769-81483788 Msa0708030:three_prime_UTR 40.0%
TCAGATTCTTGCAAAGGCTT+TGG - chr4_4:81483775-81483794 Msa0708030:three_prime_UTR 40.0%
CAGATTCTTGCAAAGGCTTT+GGG - chr4_4:81483776-81483795 Msa0708030:three_prime_UTR 40.0%
TACATCATCAATAAGTCCGG+TGG - chr4_4:81483821-81483840 Msa0708030:three_prime_UTR 40.0%
!! CGGTGGATTGATCTACTATA+AGG - chr4_4:81483838-81483857 Msa0708030:three_prime_UTR 40.0%
AGTAGAGACTAGAGAGCAAT+GGG + chr4_4:81483879-81483898 None:intergenic 40.0%
TAGTCTCTACTCTCTACTGT+GGG - chr4_4:81483888-81483907 Msa0708030:three_prime_UTR 40.0%
GCCCTCTTTGATGAATAAGT+TGG - chr4_4:81483963-81483982 Msa0708030:three_prime_UTR 40.0%
CAGCCTTGACAAATTCACAA+CGG - chr4_4:81484308-81484327 Msa0708030:intron 40.0%
! CTTTCTGAGTTAGATGGGTT+TGG + chr4_4:81484357-81484376 None:intergenic 40.0%
TGGACACCAATGATAGCTTA+CGG - chr4_4:81484499-81484518 Msa0708030:intron 40.0%
GAAGATCAAATGTGTCAGCT+TGG + chr4_4:81484605-81484624 None:intergenic 40.0%
ACATTGGTAAGTAACCTTGG+AGG - chr4_4:81484777-81484796 Msa0708030:CDS 40.0%
! ATGACCAAGGCATTACATAC+AGG + chr4_4:81484909-81484928 None:intergenic 40.0%
TGAACCTGGAACTCAACAAA+TGG - chr4_4:81485156-81485175 Msa0708030:intron 40.0%
! CTTTCCATTTGTTGAGTTCC+AGG + chr4_4:81485163-81485182 None:intergenic 40.0%
TCAAAGAGCTCACATCGTAT+AGG + chr4_4:81485256-81485275 None:intergenic 40.0%
GGCATTTCAGATTCTTGCAA+AGG - chr4_4:81483769-81483788 Msa0708030:three_prime_UTR 40.0%
TCAGATTCTTGCAAAGGCTT+TGG - chr4_4:81483775-81483794 Msa0708030:three_prime_UTR 40.0%
CAGATTCTTGCAAAGGCTTT+GGG - chr4_4:81483776-81483795 Msa0708030:three_prime_UTR 40.0%
TACATCATCAATAAGTCCGG+TGG - chr4_4:81483821-81483840 Msa0708030:three_prime_UTR 40.0%
!! CGGTGGATTGATCTACTATA+AGG - chr4_4:81483838-81483857 Msa0708030:three_prime_UTR 40.0%
AGTAGAGACTAGAGAGCAAT+GGG + chr4_4:81483879-81483898 None:intergenic 40.0%
TAGTCTCTACTCTCTACTGT+GGG - chr4_4:81483888-81483907 Msa0708030:three_prime_UTR 40.0%
GCCCTCTTTGATGAATAAGT+TGG - chr4_4:81483963-81483982 Msa0708030:three_prime_UTR 40.0%
CAGCCTTGACAAATTCACAA+CGG - chr4_4:81484308-81484327 Msa0708030:intron 40.0%
! CTTTCTGAGTTAGATGGGTT+TGG + chr4_4:81484357-81484376 None:intergenic 40.0%
TGGACACCAATGATAGCTTA+CGG - chr4_4:81484499-81484518 Msa0708030:intron 40.0%
GAAGATCAAATGTGTCAGCT+TGG + chr4_4:81484605-81484624 None:intergenic 40.0%
ACATTGGTAAGTAACCTTGG+AGG - chr4_4:81484777-81484796 Msa0708030:CDS 40.0%
! ATGACCAAGGCATTACATAC+AGG + chr4_4:81484909-81484928 None:intergenic 40.0%
TGAACCTGGAACTCAACAAA+TGG - chr4_4:81485156-81485175 Msa0708030:intron 40.0%
! CTTTCCATTTGTTGAGTTCC+AGG + chr4_4:81485163-81485182 None:intergenic 40.0%
TCAAAGAGCTCACATCGTAT+AGG + chr4_4:81485256-81485275 None:intergenic 40.0%
GAGTAGAGACTAGAGAGCAA+TGG + chr4_4:81483880-81483899 None:intergenic 45.0%
CTAGTCTCTACTCTCTACTG+TGG - chr4_4:81483887-81483906 Msa0708030:three_prime_UTR 45.0%
ACGCCGTTGTGAATTTGTCA+AGG + chr4_4:81484314-81484333 None:intergenic 45.0%
TTATGGGTCTTCTGGACGAA+TGG - chr4_4:81484479-81484498 Msa0708030:intron 45.0%
GGACACCAATGATAGCTTAC+GGG - chr4_4:81484500-81484519 Msa0708030:intron 45.0%
! CAAGGCACCTTAAATGACCA+AGG + chr4_4:81484922-81484941 None:intergenic 45.0%
GTGAACGCAAAGGCCAATTA+AGG + chr4_4:81484995-81485014 None:intergenic 45.0%
! TTTTTCGTGGTCTGTGAACC+TGG - chr4_4:81485142-81485161 Msa0708030:intron 45.0%
GAAGAACCCCTTCTATGAGA+TGG - chr4_4:81485225-81485244 Msa0708030:intron 45.0%
GCATCTCCATCTCATAGAAG+GGG + chr4_4:81485234-81485253 None:intergenic 45.0%
GGCATCTCCATCTCATAGAA+GGG + chr4_4:81485235-81485254 None:intergenic 45.0%
AGGCATCTCCATCTCATAGA+AGG + chr4_4:81485236-81485255 None:intergenic 45.0%
TCGATAAACTGCCATGGATC+TGG - chr4_4:81485324-81485343 Msa0708030:intron 45.0%
AACAACACTTGCCAGATCCA+TGG + chr4_4:81485338-81485357 None:intergenic 45.0%
GAGTAGAGACTAGAGAGCAA+TGG + chr4_4:81483880-81483899 None:intergenic 45.0%
CTAGTCTCTACTCTCTACTG+TGG - chr4_4:81483887-81483906 Msa0708030:three_prime_UTR 45.0%
ACGCCGTTGTGAATTTGTCA+AGG + chr4_4:81484314-81484333 None:intergenic 45.0%
TTATGGGTCTTCTGGACGAA+TGG - chr4_4:81484479-81484498 Msa0708030:intron 45.0%
GGACACCAATGATAGCTTAC+GGG - chr4_4:81484500-81484519 Msa0708030:intron 45.0%
! CAAGGCACCTTAAATGACCA+AGG + chr4_4:81484922-81484941 None:intergenic 45.0%
GTGAACGCAAAGGCCAATTA+AGG + chr4_4:81484995-81485014 None:intergenic 45.0%
! TTTTTCGTGGTCTGTGAACC+TGG - chr4_4:81485142-81485161 Msa0708030:intron 45.0%
GAAGAACCCCTTCTATGAGA+TGG - chr4_4:81485225-81485244 Msa0708030:intron 45.0%
GCATCTCCATCTCATAGAAG+GGG + chr4_4:81485234-81485253 None:intergenic 45.0%
GGCATCTCCATCTCATAGAA+GGG + chr4_4:81485235-81485254 None:intergenic 45.0%
AGGCATCTCCATCTCATAGA+AGG + chr4_4:81485236-81485255 None:intergenic 45.0%
TCGATAAACTGCCATGGATC+TGG - chr4_4:81485324-81485343 Msa0708030:intron 45.0%
AACAACACTTGCCAGATCCA+TGG + chr4_4:81485338-81485357 None:intergenic 45.0%
CACCAATGATAGCTTACGGG+TGG - chr4_4:81484503-81484522 Msa0708030:intron 50.0%
AGCCACCCGTAAGCTATCAT+TGG + chr4_4:81484508-81484527 None:intergenic 50.0%
AGGTGACAACTGCTGAGAGA+TGG + chr4_4:81484553-81484572 None:intergenic 50.0%
CAGCAGTTGTCACCTGTTTC+TGG - chr4_4:81484558-81484577 Msa0708030:intron 50.0%
ATTCCTGCGCAACCAGAAAC+AGG + chr4_4:81484573-81484592 None:intergenic 50.0%
CATTGCTTCCAGTCACTGAC+AGG - chr4_4:81484624-81484643 Msa0708030:intron 50.0%
TGGTAACAACTGGTGCAGCA+AGG + chr4_4:81484940-81484959 None:intergenic 50.0%
CAGCATCACAGTGAACGCAA+AGG + chr4_4:81485005-81485024 None:intergenic 50.0%
CACGAAAAACTTCGTCCCTG+TGG + chr4_4:81485131-81485150 None:intergenic 50.0%
! CACAGGGACGAAGTTTTTCG+TGG - chr4_4:81485129-81485148 Msa0708030:intron 50.0%
AAGGACCGTGTTGCATTGCT+GGG - chr4_4:81485301-81485320 Msa0708030:intron 50.0%
ATCGACCCAGCAATGCAACA+CGG + chr4_4:81485309-81485328 None:intergenic 50.0%
CACCAATGATAGCTTACGGG+TGG - chr4_4:81484503-81484522 Msa0708030:intron 50.0%
AGCCACCCGTAAGCTATCAT+TGG + chr4_4:81484508-81484527 None:intergenic 50.0%
AGGTGACAACTGCTGAGAGA+TGG + chr4_4:81484553-81484572 None:intergenic 50.0%
CAGCAGTTGTCACCTGTTTC+TGG - chr4_4:81484558-81484577 Msa0708030:intron 50.0%
ATTCCTGCGCAACCAGAAAC+AGG + chr4_4:81484573-81484592 None:intergenic 50.0%
CATTGCTTCCAGTCACTGAC+AGG - chr4_4:81484624-81484643 Msa0708030:intron 50.0%
TGGTAACAACTGGTGCAGCA+AGG + chr4_4:81484940-81484959 None:intergenic 50.0%
CAGCATCACAGTGAACGCAA+AGG + chr4_4:81485005-81485024 None:intergenic 50.0%
CACGAAAAACTTCGTCCCTG+TGG + chr4_4:81485131-81485150 None:intergenic 50.0%
! CACAGGGACGAAGTTTTTCG+TGG - chr4_4:81485129-81485148 Msa0708030:intron 50.0%
AAGGACCGTGTTGCATTGCT+GGG - chr4_4:81485301-81485320 Msa0708030:intron 50.0%
ATCGACCCAGCAATGCAACA+CGG + chr4_4:81485309-81485328 None:intergenic 50.0%
!! GCTTACGGGTGGCTAGCTTA+TGG - chr4_4:81484514-81484533 Msa0708030:intron 55.0%
!! TCACCTGTTTCTGGTTGCGC+AGG - chr4_4:81484567-81484586 Msa0708030:intron 55.0%
TGCACAGTACAGTGCCTCCA+AGG + chr4_4:81484794-81484813 None:intergenic 55.0%
GTACTGTGCAGTCTGTGCTG+TGG - chr4_4:81484804-81484823 Msa0708030:CDS 55.0%
CTTAGCTCAGGCAGTGCAGA+AGG - chr4_4:81485282-81485301 Msa0708030:intron 55.0%
GAAGGACCGTGTTGCATTGC+TGG - chr4_4:81485300-81485319 Msa0708030:intron 55.0%
GCTGGGTCGATAAACTGCCA+TGG - chr4_4:81485318-81485337 Msa0708030:intron 55.0%
!! GCTTACGGGTGGCTAGCTTA+TGG - chr4_4:81484514-81484533 Msa0708030:intron 55.0%
!! TCACCTGTTTCTGGTTGCGC+AGG - chr4_4:81484567-81484586 Msa0708030:intron 55.0%
TGCACAGTACAGTGCCTCCA+AGG + chr4_4:81484794-81484813 None:intergenic 55.0%
GTACTGTGCAGTCTGTGCTG+TGG - chr4_4:81484804-81484823 Msa0708030:CDS 55.0%
CTTAGCTCAGGCAGTGCAGA+AGG - chr4_4:81485282-81485301 Msa0708030:intron 55.0%
GAAGGACCGTGTTGCATTGC+TGG - chr4_4:81485300-81485319 Msa0708030:intron 55.0%
GCTGGGTCGATAAACTGCCA+TGG - chr4_4:81485318-81485337 Msa0708030:intron 55.0%
TGTGCAGTCTGTGCTGTGGC+TGG - chr4_4:81484808-81484827 Msa0708030:CDS 60.0%
GTGCAGTCTGTGCTGTGGCT+GGG - chr4_4:81484809-81484828 Msa0708030:CDS 60.0%
TGTGCAGTCTGTGCTGTGGC+TGG - chr4_4:81484808-81484827 Msa0708030:CDS 60.0%
GTGCAGTCTGTGCTGTGGCT+GGG - chr4_4:81484809-81484828 Msa0708030:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81483757 81485567 81483757 ID=Msa0708030;Name=Msa0708030
chr4_4 mRNA 81483757 81485567 81483757 ID=Msa0708030-mRNA-1;Parent=Msa0708030;Name=Msa0708030-mRNA-1;_AED=0.06;_eAED=0.06;_QI=10|1|1|1|1|1|3|237|152
chr4_4 exon 81483757 81484190 81483757 ID=Msa0708030-mRNA-1:exon:26652;Parent=Msa0708030-mRNA-1
chr4_4 exon 81484679 81484847 81484679 ID=Msa0708030-mRNA-1:exon:26651;Parent=Msa0708030-mRNA-1
chr4_4 exon 81485465 81485567 81485465 ID=Msa0708030-mRNA-1:exon:26650;Parent=Msa0708030-mRNA-1
chr4_4 five_prime_UTR 81485558 81485567 81485558 ID=Msa0708030-mRNA-1:five_prime_utr;Parent=Msa0708030-mRNA-1
chr4_4 CDS 81485465 81485557 81485465 ID=Msa0708030-mRNA-1:cds;Parent=Msa0708030-mRNA-1
chr4_4 CDS 81484679 81484847 81484679 ID=Msa0708030-mRNA-1:cds;Parent=Msa0708030-mRNA-1
chr4_4 CDS 81483994 81484190 81483994 ID=Msa0708030-mRNA-1:cds;Parent=Msa0708030-mRNA-1
chr4_4 three_prime_UTR 81483757 81483993 81483757 ID=Msa0708030-mRNA-1:three_prime_utr;Parent=Msa0708030-mRNA-1
Gene Sequence

>Msa0708030

ATGGCATTTCAGATTCTTGCAAAGGCTTTGGGAATGACAGCAGTTTACAGTCTTTACATCATCAATAAGTCCGGTGGATTGATCTACTATAAGGATTATGGGTCTTCTGGACGAATGGACACCAATGATAGCTTACGGGTGGCTAGCTTATGGCATTCAATGCATGCCATCTCTCAGCAGTTGTCACCTGTTTCTGGTTGCGCAGGAATTGAATTACTCCAAGCTGACACATTTGATCTTCATTGCTTCCAGTCACTGACAGGGACGAAGTTTTTCGTGGTCTGTGAACCTGGAACTCAACAAATGGAAAGTTTACTGAAATTCATATATGAACTGTACACAGATTATGTCTTGAAGAACCCCTTCTATGAGATGGAGATGCCTATACGATGTGAGCTCTTTGATATCAACTTAGCTCAGGCAGTGCAGAAGGACCGTGTTGCATTGCTGGGTCGATAA
Protein sequence

>Msa0708030

MAFQILAKALGMTAVYSLYIINKSGGLIYYKDYGSSGRMDTNDSLRVASLWHSMHAISQQLSPVSGCAGIELLQADTFDLHCFQSLTGTKFFVVCEPGTQQMESLLKFIYELYTDYVLKNPFYEMEMPIRCELFDINLAQAVQKDRVALLGR*