AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0711520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0711520 MtrunA17_Chr5g0394371 98.193 166 3 0 1 166 163 328 2.33e-120 342
Msa0711520 MtrunA17_Chr3g0136181 63.804 163 59 0 1 163 61 223 3.22e-77 229
Msa0711520 MtrunA17_Chr1g0150851 63.804 163 59 0 1 163 180 342 1.51e-74 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0711520 AT5G19530.1 61.350 163 63 0 1 163 171 333 3.57e-74 226
Msa0711520 AT5G19530.2 61.350 163 63 0 1 163 164 326 3.62e-74 225

Find 56 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCATCTTATGCTGATATT+TGG 0.118417 5_1:-3400961 Msa0711520:CDS
TCCTATCTTGTGCTTCTATT+TGG 0.195443 5_1:-3400331 Msa0711520:three_prime_UTR
CCCATCTTATGCTGATATTT+GGG 0.239153 5_1:-3400960 Msa0711520:CDS
ATTCCACCTTTCTTCAGTTT+TGG 0.286598 5_1:+3401317 None:intergenic
ACCTATGATAACAAAAGTTT+AGG 0.330445 5_1:+3400170 None:intergenic
TAGCAAGGACCTCCTTCTAT+TGG 0.333191 5_1:+3401380 None:intergenic
TTTAATATCGGTCAAATACT+TGG 0.356177 5_1:+3400207 None:intergenic
CCTATGATAACAAAAGTTTA+GGG 0.357870 5_1:+3400171 None:intergenic
CTTATGCAGGCAGGGCCTGC+TGG 0.371312 5_1:-3401180 Msa0711520:intron
TGATGTAATAGTTGGTGATT+TGG 0.391967 5_1:-3401408 Msa0711520:CDS
GATGGGATATGAGCTGAATA+AGG 0.405736 5_1:+3400977 None:intergenic
TAGGTAGCCAATGTTTGGTT+TGG 0.410891 5_1:-3400152 Msa0711520:three_prime_UTR
TGATGTTGCGAGTTTAATAT+CGG 0.418972 5_1:+3400195 None:intergenic
ACACTTGAAAACTTGTCTTA+AGG 0.423360 5_1:+3401116 None:intergenic
ATATATTAGAGTAGTGGTAT+AGG 0.425436 5_1:+3400296 None:intergenic
AGTGAGCTCATAAAAGTCTT+TGG 0.426867 5_1:+3401346 None:intergenic
GAAGAGAATAGATATCTTGA+TGG 0.427990 5_1:-3400762 Msa0711520:CDS
TCCAAATAGAAGCACAAGAT+AGG 0.429219 5_1:+3400330 None:intergenic
GGAGCAGCTAGGTTCATATA+TGG 0.430922 5_1:-3400568 Msa0711520:CDS
TATCATAGGTAGCCAATGTT+TGG 0.456758 5_1:-3400157 Msa0711520:three_prime_UTR
GAGAGGTATGATGTAATAGT+TGG 0.464597 5_1:-3401416 Msa0711520:CDS
CCCCAAATATCAGCATAAGA+TGG 0.466906 5_1:+3400959 None:intergenic
TGATATTTGGGGATGGGTCC+TGG 0.469083 5_1:-3400948 Msa0711520:intron
TTATGCTGATATTTGGGGAT+GGG 0.480221 5_1:-3400954 Msa0711520:CDS
AAGCACAAGATAGGAATCAT+TGG 0.491928 5_1:+3400339 None:intergenic
GATTTGGCTGATCCAATAGA+AGG 0.493154 5_1:-3401392 Msa0711520:CDS
CTTATGCTGATATTTGGGGA+TGG 0.502652 5_1:-3400955 Msa0711520:CDS
TGTTACAGAGCTGAACTAGA+AGG 0.509621 5_1:-3401446 None:intergenic
CCCAAATATCAGCATAAGAT+GGG 0.522916 5_1:+3400960 None:intergenic
CTCAAGCCAAAACTGAAGAA+AGG 0.529230 5_1:-3401323 Msa0711520:CDS
TATCTAATTCTTCAGCACTA+AGG 0.537029 5_1:+3400808 None:intergenic
AAAGAAAGTGAAGAGCAGAA+AGG 0.542919 5_1:+3400428 None:intergenic
ACAGGCTTCAGATTCTCCAT+TGG 0.548267 5_1:-3400832 Msa0711520:intron
TTAATATCGGTCAAATACTT+GGG 0.554417 5_1:+3400208 None:intergenic
GTGTGGCTGAATATTCCAGC+AGG 0.556371 5_1:+3401165 None:intergenic
CCATCTTATGCTGATATTTG+GGG 0.559813 5_1:-3400959 Msa0711520:CDS
TTGTCAAACACGAGTAAAAG+TGG 0.564885 5_1:+3400122 None:intergenic
AATTAAATATATTAGAGTAG+TGG 0.569401 5_1:+3400290 None:intergenic
TCTTCAGCACTAAGGTCCAA+TGG 0.578062 5_1:+3400816 None:intergenic
TTGGTGTAAAGTTTATAGCA+AGG 0.578661 5_1:+3401365 None:intergenic
GCTAGGTTCATATATGGTCA+TGG 0.584533 5_1:-3400562 Msa0711520:CDS
AGGTGGAATATTTGTGACAC+AGG 0.588611 5_1:-3401303 Msa0711520:intron
AGTGGTGAAAGTACAAGCCA+AGG 0.593535 5_1:+3400398 None:intergenic
AACTAGAAGGCAAAGAAGAG+AGG 0.603737 5_1:-3401433 Msa0711520:CDS
GTGGTGAAAGTACAAGCCAA+GGG 0.607505 5_1:+3400399 None:intergenic
AAGCCAAAACTGAAGAAAGG+TGG 0.618874 5_1:-3401320 Msa0711520:CDS
CAAACACGAGTAAAAGTGGT+TGG 0.631173 5_1:+3400126 None:intergenic
CACACAATGCAAGTTAACAA+AGG 0.637771 5_1:-3400487 Msa0711520:three_prime_UTR
GAGACTCATGTATACACAGA+GGG 0.651343 5_1:-3400589 Msa0711520:CDS
TTGGTCGCCAAACCAAACAT+TGG 0.652868 5_1:+3400145 None:intergenic
TATACACAGAGGGAGCAGCT+AGG 0.656967 5_1:-3400579 Msa0711520:CDS
TAGATATCAGAATGAGACAG+AGG 0.660960 5_1:-3400791 Msa0711520:CDS
TTGGCTGATCCAATAGAAGG+AGG 0.689346 5_1:-3401389 Msa0711520:CDS
TACATGAGAAAACTTCTGTG+TGG 0.723090 5_1:+3401148 None:intergenic
TGGTGAAAGTACAAGCCAAG+GGG 0.731603 5_1:+3400400 None:intergenic
TGAGACTCATGTATACACAG+AGG 0.767768 5_1:-3400590 Msa0711520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAATTTAAGAAAAAATG+TGG - chr5_1:3401297-3401316 Msa0711520:intron 10.0%
!!! ATTTAATTTAAGAAAAAATG+TGG - chr5_1:3401297-3401316 Msa0711520:intron 10.0%
!! ATGTATTAAAAAACTTGATA+AGG - chr5_1:3400327-3400346 Msa0711520:three_prime_UTR 15.0%
!! CAATAATATATAAATTTACC+AGG + chr5_1:3400645-3400664 None:intergenic 15.0%
!! AATTAAATATATTAGAGTAG+TGG + chr5_1:3401285-3401304 None:intergenic 15.0%
!!! TGATTTTGTTTTGTTTATTA+TGG - chr5_1:3401324-3401343 Msa0711520:CDS 15.0%
!! ATGTATTAAAAAACTTGATA+AGG - chr5_1:3400327-3400346 Msa0711520:three_prime_UTR 15.0%
!! CAATAATATATAAATTTACC+AGG + chr5_1:3400645-3400664 None:intergenic 15.0%
!! AATTAAATATATTAGAGTAG+TGG + chr5_1:3401285-3401304 None:intergenic 15.0%
!!! TGATTTTGTTTTGTTTATTA+TGG - chr5_1:3401324-3401343 Msa0711520:CDS 15.0%
!! AAACATAGAATACTTAGATT+TGG + chr5_1:3400704-3400723 None:intergenic 20.0%
!! AAACATAGAATACTTAGATT+TGG + chr5_1:3400704-3400723 None:intergenic 20.0%
!! TGTTGTTATTATTCTTATGC+AGG - chr5_1:3400379-3400398 Msa0711520:three_prime_UTR 25.0%
!!! TTTTGTTTTGATTTGAAGCT+TGG + chr5_1:3401049-3401068 None:intergenic 25.0%
! ATATATTAGAGTAGTGGTAT+AGG + chr5_1:3401279-3401298 None:intergenic 25.0%
! TTAATATCGGTCAAATACTT+GGG + chr5_1:3401367-3401386 None:intergenic 25.0%
! TTTAATATCGGTCAAATACT+TGG + chr5_1:3401368-3401387 None:intergenic 25.0%
! CCTATGATAACAAAAGTTTA+GGG + chr5_1:3401404-3401423 None:intergenic 25.0%
! ACCTATGATAACAAAAGTTT+AGG + chr5_1:3401405-3401424 None:intergenic 25.0%
!! TGTTGTTATTATTCTTATGC+AGG - chr5_1:3400379-3400398 Msa0711520:three_prime_UTR 25.0%
!!! TTTTGTTTTGATTTGAAGCT+TGG + chr5_1:3401049-3401068 None:intergenic 25.0%
! ATATATTAGAGTAGTGGTAT+AGG + chr5_1:3401279-3401298 None:intergenic 25.0%
! TTAATATCGGTCAAATACTT+GGG + chr5_1:3401367-3401386 None:intergenic 25.0%
! TTTAATATCGGTCAAATACT+TGG + chr5_1:3401368-3401387 None:intergenic 25.0%
! CCTATGATAACAAAAGTTTA+GGG + chr5_1:3401404-3401423 None:intergenic 25.0%
! ACCTATGATAACAAAAGTTT+AGG + chr5_1:3401405-3401424 None:intergenic 25.0%
!! TGATGTAATAGTTGGTGATT+TGG - chr5_1:3400164-3400183 Msa0711520:three_prime_UTR 30.0%
TTGGTGTAAAGTTTATAGCA+AGG + chr5_1:3400210-3400229 None:intergenic 30.0%
TTATTATTCTTATGCAGGCA+GGG - chr5_1:3400384-3400403 Msa0711520:three_prime_UTR 30.0%
ACACTTGAAAACTTGTCTTA+AGG + chr5_1:3400459-3400478 None:intergenic 30.0%
!!! GTATTCTATGTTTTTTCCCT+TGG - chr5_1:3400711-3400730 Msa0711520:intron 30.0%
TATCTAATTCTTCAGCACTA+AGG + chr5_1:3400767-3400786 None:intergenic 30.0%
GAAGAGAATAGATATCTTGA+TGG - chr5_1:3400810-3400829 Msa0711520:CDS 30.0%
CATTGAATGATACTATTGAC+AGG - chr5_1:3400951-3400970 Msa0711520:CDS 30.0%
TACTTTCACCACTCAAAAAA+AGG - chr5_1:3401184-3401203 Msa0711520:intron 30.0%
TGATGTTGCGAGTTTAATAT+CGG + chr5_1:3401380-3401399 None:intergenic 30.0%
!! CCCTAAACTTTTGTTATCAT+AGG - chr5_1:3401401-3401420 Msa0711520:CDS 30.0%
!! TGATGTAATAGTTGGTGATT+TGG - chr5_1:3400164-3400183 Msa0711520:three_prime_UTR 30.0%
TTGGTGTAAAGTTTATAGCA+AGG + chr5_1:3400210-3400229 None:intergenic 30.0%
TTATTATTCTTATGCAGGCA+GGG - chr5_1:3400384-3400403 Msa0711520:three_prime_UTR 30.0%
ACACTTGAAAACTTGTCTTA+AGG + chr5_1:3400459-3400478 None:intergenic 30.0%
!!! GTATTCTATGTTTTTTCCCT+TGG - chr5_1:3400711-3400730 Msa0711520:intron 30.0%
TATCTAATTCTTCAGCACTA+AGG + chr5_1:3400767-3400786 None:intergenic 30.0%
GAAGAGAATAGATATCTTGA+TGG - chr5_1:3400810-3400829 Msa0711520:CDS 30.0%
CATTGAATGATACTATTGAC+AGG - chr5_1:3400951-3400970 Msa0711520:CDS 30.0%
TACTTTCACCACTCAAAAAA+AGG - chr5_1:3401184-3401203 Msa0711520:intron 30.0%
TGATGTTGCGAGTTTAATAT+CGG + chr5_1:3401380-3401399 None:intergenic 30.0%
!! CCCTAAACTTTTGTTATCAT+AGG - chr5_1:3401401-3401420 Msa0711520:CDS 30.0%
! GAGAGGTATGATGTAATAGT+TGG - chr5_1:3400156-3400175 Msa0711520:three_prime_UTR 35.0%
AGTGAGCTCATAAAAGTCTT+TGG + chr5_1:3400229-3400248 None:intergenic 35.0%
! ATTCCACCTTTCTTCAGTTT+TGG + chr5_1:3400258-3400277 None:intergenic 35.0%
GTTATTATTCTTATGCAGGC+AGG - chr5_1:3400383-3400402 Msa0711520:three_prime_UTR 35.0%
TACATGAGAAAACTTCTGTG+TGG + chr5_1:3400427-3400446 None:intergenic 35.0%
TCTGCAAACATGAAGAAAGT+AGG + chr5_1:3400568-3400587 None:intergenic 35.0%
TTTCTTCATGTTTGCAGATG+TGG - chr5_1:3400570-3400589 Msa0711520:CDS 35.0%
TCCCATCTTATGCTGATATT+TGG - chr5_1:3400611-3400630 Msa0711520:intron 35.0%
CCCAAATATCAGCATAAGAT+GGG + chr5_1:3400615-3400634 None:intergenic 35.0%
CCCATCTTATGCTGATATTT+GGG - chr5_1:3400612-3400631 Msa0711520:intron 35.0%
CCATCTTATGCTGATATTTG+GGG - chr5_1:3400613-3400632 Msa0711520:intron 35.0%
TTATGCTGATATTTGGGGAT+GGG - chr5_1:3400618-3400637 Msa0711520:intron 35.0%
TAGATATCAGAATGAGACAG+AGG - chr5_1:3400781-3400800 Msa0711520:CDS 35.0%
CACACAATGCAAGTTAACAA+AGG - chr5_1:3401085-3401104 Msa0711520:intron 35.0%
AAAGAAAGTGAAGAGCAGAA+AGG + chr5_1:3401147-3401166 None:intergenic 35.0%
!!! CACTTTCTTTTTTTACCCCT+TGG - chr5_1:3401157-3401176 Msa0711520:CDS 35.0%
!!! CTTACAAGCCTTTTTTTGAG+TGG + chr5_1:3401195-3401214 None:intergenic 35.0%
AAGCACAAGATAGGAATCAT+TGG + chr5_1:3401236-3401255 None:intergenic 35.0%
TCCTATCTTGTGCTTCTATT+TGG - chr5_1:3401241-3401260 Msa0711520:intron 35.0%
! TCCAAATAGAAGCACAAGAT+AGG + chr5_1:3401245-3401264 None:intergenic 35.0%
TATCATAGGTAGCCAATGTT+TGG - chr5_1:3401415-3401434 Msa0711520:CDS 35.0%
! GAGAGGTATGATGTAATAGT+TGG - chr5_1:3400156-3400175 Msa0711520:three_prime_UTR 35.0%
AGTGAGCTCATAAAAGTCTT+TGG + chr5_1:3400229-3400248 None:intergenic 35.0%
! ATTCCACCTTTCTTCAGTTT+TGG + chr5_1:3400258-3400277 None:intergenic 35.0%
GTTATTATTCTTATGCAGGC+AGG - chr5_1:3400383-3400402 Msa0711520:three_prime_UTR 35.0%
TACATGAGAAAACTTCTGTG+TGG + chr5_1:3400427-3400446 None:intergenic 35.0%
TCTGCAAACATGAAGAAAGT+AGG + chr5_1:3400568-3400587 None:intergenic 35.0%
TTTCTTCATGTTTGCAGATG+TGG - chr5_1:3400570-3400589 Msa0711520:CDS 35.0%
TCCCATCTTATGCTGATATT+TGG - chr5_1:3400611-3400630 Msa0711520:intron 35.0%
CCCAAATATCAGCATAAGAT+GGG + chr5_1:3400615-3400634 None:intergenic 35.0%
CCCATCTTATGCTGATATTT+GGG - chr5_1:3400612-3400631 Msa0711520:intron 35.0%
CCATCTTATGCTGATATTTG+GGG - chr5_1:3400613-3400632 Msa0711520:intron 35.0%
TTATGCTGATATTTGGGGAT+GGG - chr5_1:3400618-3400637 Msa0711520:intron 35.0%
TAGATATCAGAATGAGACAG+AGG - chr5_1:3400781-3400800 Msa0711520:CDS 35.0%
CACACAATGCAAGTTAACAA+AGG - chr5_1:3401085-3401104 Msa0711520:intron 35.0%
AAAGAAAGTGAAGAGCAGAA+AGG + chr5_1:3401147-3401166 None:intergenic 35.0%
!!! CACTTTCTTTTTTTACCCCT+TGG - chr5_1:3401157-3401176 Msa0711520:CDS 35.0%
!!! CTTACAAGCCTTTTTTTGAG+TGG + chr5_1:3401195-3401214 None:intergenic 35.0%
AAGCACAAGATAGGAATCAT+TGG + chr5_1:3401236-3401255 None:intergenic 35.0%
TCCTATCTTGTGCTTCTATT+TGG - chr5_1:3401241-3401260 Msa0711520:intron 35.0%
! TCCAAATAGAAGCACAAGAT+AGG + chr5_1:3401245-3401264 None:intergenic 35.0%
TATCATAGGTAGCCAATGTT+TGG - chr5_1:3401415-3401434 Msa0711520:CDS 35.0%
AACTAGAAGGCAAAGAAGAG+AGG - chr5_1:3400139-3400158 Msa0711520:three_prime_UTR 40.0%
GATTTGGCTGATCCAATAGA+AGG - chr5_1:3400180-3400199 Msa0711520:three_prime_UTR 40.0%
CTCAAGCCAAAACTGAAGAA+AGG - chr5_1:3400249-3400268 Msa0711520:three_prime_UTR 40.0%
AAGCCAAAACTGAAGAAAGG+TGG - chr5_1:3400252-3400271 Msa0711520:three_prime_UTR 40.0%
AGGTGGAATATTTGTGACAC+AGG - chr5_1:3400269-3400288 Msa0711520:three_prime_UTR 40.0%
GGAATATTTGTGACACAGGT+AGG - chr5_1:3400273-3400292 Msa0711520:three_prime_UTR 40.0%
ATATTTGTGACACAGGTAGG+TGG - chr5_1:3400276-3400295 Msa0711520:three_prime_UTR 40.0%
GATGGGATATGAGCTGAATA+AGG + chr5_1:3400598-3400617 None:intergenic 40.0%
CCCCAAATATCAGCATAAGA+TGG + chr5_1:3400616-3400635 None:intergenic 40.0%
CTTATGCTGATATTTGGGGA+TGG - chr5_1:3400617-3400636 Msa0711520:intron 40.0%
! TTTTTCCCTTGGCCTATAAC+AGG - chr5_1:3400722-3400741 Msa0711520:CDS 40.0%
! GAGAATCTGAAGCCTGTTAT+AGG + chr5_1:3400737-3400756 None:intergenic 40.0%
GTTCGTAACTCGTAAGCAAA+TGG - chr5_1:3400870-3400889 Msa0711520:intron 40.0%
TGAGACTCATGTATACACAG+AGG - chr5_1:3400982-3401001 Msa0711520:CDS 40.0%
GAGACTCATGTATACACAGA+GGG - chr5_1:3400983-3401002 Msa0711520:CDS 40.0%
GCTAGGTTCATATATGGTCA+TGG - chr5_1:3401010-3401029 Msa0711520:intron 40.0%
TAGGTAGCCAATGTTTGGTT+TGG - chr5_1:3401420-3401439 Msa0711520:CDS 40.0%
AACTAGAAGGCAAAGAAGAG+AGG - chr5_1:3400139-3400158 Msa0711520:three_prime_UTR 40.0%
GATTTGGCTGATCCAATAGA+AGG - chr5_1:3400180-3400199 Msa0711520:three_prime_UTR 40.0%
CTCAAGCCAAAACTGAAGAA+AGG - chr5_1:3400249-3400268 Msa0711520:three_prime_UTR 40.0%
AAGCCAAAACTGAAGAAAGG+TGG - chr5_1:3400252-3400271 Msa0711520:three_prime_UTR 40.0%
AGGTGGAATATTTGTGACAC+AGG - chr5_1:3400269-3400288 Msa0711520:three_prime_UTR 40.0%
GGAATATTTGTGACACAGGT+AGG - chr5_1:3400273-3400292 Msa0711520:three_prime_UTR 40.0%
ATATTTGTGACACAGGTAGG+TGG - chr5_1:3400276-3400295 Msa0711520:three_prime_UTR 40.0%
GATGGGATATGAGCTGAATA+AGG + chr5_1:3400598-3400617 None:intergenic 40.0%
CCCCAAATATCAGCATAAGA+TGG + chr5_1:3400616-3400635 None:intergenic 40.0%
CTTATGCTGATATTTGGGGA+TGG - chr5_1:3400617-3400636 Msa0711520:intron 40.0%
! TTTTTCCCTTGGCCTATAAC+AGG - chr5_1:3400722-3400741 Msa0711520:CDS 40.0%
! GAGAATCTGAAGCCTGTTAT+AGG + chr5_1:3400737-3400756 None:intergenic 40.0%
GTTCGTAACTCGTAAGCAAA+TGG - chr5_1:3400870-3400889 Msa0711520:intron 40.0%
TGAGACTCATGTATACACAG+AGG - chr5_1:3400982-3401001 Msa0711520:CDS 40.0%
GAGACTCATGTATACACAGA+GGG - chr5_1:3400983-3401002 Msa0711520:CDS 40.0%
GCTAGGTTCATATATGGTCA+TGG - chr5_1:3401010-3401029 Msa0711520:intron 40.0%
TAGGTAGCCAATGTTTGGTT+TGG - chr5_1:3401420-3401439 Msa0711520:CDS 40.0%
TTGGCTGATCCAATAGAAGG+AGG - chr5_1:3400183-3400202 Msa0711520:three_prime_UTR 45.0%
TAGCAAGGACCTCCTTCTAT+TGG + chr5_1:3400195-3400214 None:intergenic 45.0%
TGAAGCCTGTTATAGGCCAA+GGG + chr5_1:3400730-3400749 None:intergenic 45.0%
ACAGGCTTCAGATTCTCCAT+TGG - chr5_1:3400740-3400759 Msa0711520:CDS 45.0%
TCTTCAGCACTAAGGTCCAA+TGG + chr5_1:3400759-3400778 None:intergenic 45.0%
GGAGCAGCTAGGTTCATATA+TGG - chr5_1:3401004-3401023 Msa0711520:intron 45.0%
! TGGTGAAAGTACAAGCCAAG+GGG + chr5_1:3401175-3401194 None:intergenic 45.0%
! GTGGTGAAAGTACAAGCCAA+GGG + chr5_1:3401176-3401195 None:intergenic 45.0%
! AGTGGTGAAAGTACAAGCCA+AGG + chr5_1:3401177-3401196 None:intergenic 45.0%
TTGGTCGCCAAACCAAACAT+TGG + chr5_1:3401430-3401449 None:intergenic 45.0%
TTGGCTGATCCAATAGAAGG+AGG - chr5_1:3400183-3400202 Msa0711520:three_prime_UTR 45.0%
TAGCAAGGACCTCCTTCTAT+TGG + chr5_1:3400195-3400214 None:intergenic 45.0%
TGAAGCCTGTTATAGGCCAA+GGG + chr5_1:3400730-3400749 None:intergenic 45.0%
ACAGGCTTCAGATTCTCCAT+TGG - chr5_1:3400740-3400759 Msa0711520:CDS 45.0%
TCTTCAGCACTAAGGTCCAA+TGG + chr5_1:3400759-3400778 None:intergenic 45.0%
GGAGCAGCTAGGTTCATATA+TGG - chr5_1:3401004-3401023 Msa0711520:intron 45.0%
! TGGTGAAAGTACAAGCCAAG+GGG + chr5_1:3401175-3401194 None:intergenic 45.0%
! GTGGTGAAAGTACAAGCCAA+GGG + chr5_1:3401176-3401195 None:intergenic 45.0%
! AGTGGTGAAAGTACAAGCCA+AGG + chr5_1:3401177-3401196 None:intergenic 45.0%
TTGGTCGCCAAACCAAACAT+TGG + chr5_1:3401430-3401449 None:intergenic 45.0%
TTTGTGACACAGGTAGGTGG+CGG - chr5_1:3400279-3400298 Msa0711520:three_prime_UTR 50.0%
GTGTGGCTGAATATTCCAGC+AGG + chr5_1:3400410-3400429 None:intergenic 50.0%
TGATATTTGGGGATGGGTCC+TGG - chr5_1:3400624-3400643 Msa0711520:intron 50.0%
CTGAAGCCTGTTATAGGCCA+AGG + chr5_1:3400731-3400750 None:intergenic 50.0%
TATACACAGAGGGAGCAGCT+AGG - chr5_1:3400993-3401012 Msa0711520:intron 50.0%
TTTGTGACACAGGTAGGTGG+CGG - chr5_1:3400279-3400298 Msa0711520:three_prime_UTR 50.0%
GTGTGGCTGAATATTCCAGC+AGG + chr5_1:3400410-3400429 None:intergenic 50.0%
TGATATTTGGGGATGGGTCC+TGG - chr5_1:3400624-3400643 Msa0711520:intron 50.0%
CTGAAGCCTGTTATAGGCCA+AGG + chr5_1:3400731-3400750 None:intergenic 50.0%
TATACACAGAGGGAGCAGCT+AGG - chr5_1:3400993-3401012 Msa0711520:intron 50.0%
! GTGACACAGGTAGGTGGCGG+AGG - chr5_1:3400282-3400301 Msa0711520:three_prime_UTR 65.0%
!! ACACAGGTAGGTGGCGGAGG+AGG - chr5_1:3400285-3400304 Msa0711520:three_prime_UTR 65.0%
CTTATGCAGGCAGGGCCTGC+TGG - chr5_1:3400392-3400411 Msa0711520:three_prime_UTR 65.0%
! GTGACACAGGTAGGTGGCGG+AGG - chr5_1:3400282-3400301 Msa0711520:three_prime_UTR 65.0%
!! ACACAGGTAGGTGGCGGAGG+AGG - chr5_1:3400285-3400304 Msa0711520:three_prime_UTR 65.0%
CTTATGCAGGCAGGGCCTGC+TGG - chr5_1:3400392-3400411 Msa0711520:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 3400135 3401459 3400135 ID=Msa0711520;Name=Msa0711520
chr5_1 mRNA 3400135 3401459 3400135 ID=Msa0711520-mRNA-1;Parent=Msa0711520;Name=Msa0711520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|1|1|1|1|0.8|5|414|166
chr5_1 exon 3400135 3400621 3400135 ID=Msa0711520-mRNA-1:exon:1291;Parent=Msa0711520-mRNA-1
chr5_1 exon 3400714 3400850 3400714 ID=Msa0711520-mRNA-1:exon:1290;Parent=Msa0711520-mRNA-1
chr5_1 exon 3400949 3401007 3400949 ID=Msa0711520-mRNA-1:exon:1289;Parent=Msa0711520-mRNA-1
chr5_1 exon 3401118 3401193 3401118 ID=Msa0711520-mRNA-1:exon:1288;Parent=Msa0711520-mRNA-1
chr5_1 exon 3401304 3401459 3401304 ID=Msa0711520-mRNA-1:exon:1287;Parent=Msa0711520-mRNA-1
chr5_1 CDS 3401304 3401459 3401304 ID=Msa0711520-mRNA-1:cds;Parent=Msa0711520-mRNA-1
chr5_1 CDS 3401118 3401193 3401118 ID=Msa0711520-mRNA-1:cds;Parent=Msa0711520-mRNA-1
chr5_1 CDS 3400949 3401007 3400949 ID=Msa0711520-mRNA-1:cds;Parent=Msa0711520-mRNA-1
chr5_1 CDS 3400714 3400850 3400714 ID=Msa0711520-mRNA-1:cds;Parent=Msa0711520-mRNA-1
chr5_1 CDS 3400549 3400621 3400549 ID=Msa0711520-mRNA-1:cds;Parent=Msa0711520-mRNA-1
chr5_1 three_prime_UTR 3400135 3400548 3400135 ID=Msa0711520-mRNA-1:three_prime_utr;Parent=Msa0711520-mRNA-1
Gene Sequence

>Msa0711520

GCTGAACTAGAAGGCAAAGAAGAGAGGTATGATGTAATAGTTGGTGATTTGGCTGATCCAATAGAAGGAGGTCCTTGCTATAAACTTTACACCAAAGACTTTTATGAGCTCACTCTCAAGCCAAAACTGAAGAAAGGTGGAATATTTGTGACACAGGCAGGGCCTGCTGGAATATTCAGCCACACAGAAGTTTTCTCATGTATTTACAACACCTTAAGACAAGTTTTCAAGTATGTGGTGCCTTATTCAGCTCATATCCCATCTTATGCTGATATTTGGGGATGGGTCCTGGCTTCAGATTCTCCATTGGACCTTAGTGCTGAAGAATTAGATATCAGAATGAGACAGAGGATTGTTGAAGAGAATAGATATCTTGATGGAAAAACATTTGTGTCATCATCAACTTTGAGCAAAGTTGTTCGTAACTCGCTAAACAATGAGACTCATGTATACACAGAGGGAGCAGCTAGGTTCATATATGGTCATGGAAAGAATGCATAA
Protein sequence

>Msa0711520

AELEGKEERYDVIVGDLADPIEGGPCYKLYTKDFYELTLKPKLKKGGIFVTQAGPAGIFSHTEVFSCIYNTLRQVFKYVVPYSAHIPSYADIWGWVLASDSPLDLSAEELDIRMRQRIVEENRYLDGKTFVSSSTLSKVVRNSLNNETHVYTEGAARFIYGHGKNA*